CSAD cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Gene Symbol	CSAD
Entrez Gene ID	492476
Full Name	cysteine sulfinic acid decarboxylase
Synonyms	zgc:103478
General protein information	Preferred Names cysteine sulfinic acid decarboxylase Names cysteine sulfinic acid decarboxylase
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007348.1	NP_001007349.1	cysteine sulfinic acid decarboxylase
XM_009297043.3	XP_009295318.1	cysteine sulfinic acid decarboxylase isoform X1
XM_009297043.2	XP_009295318.1	cysteine sulfinic acid decarboxylase isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	csad	XP_002936687.2
Pan troglodytes (chimpanzee)	CSAD	XP_003313720.1
Gallus gallus (chicken)	CSAD	XP_423847.3
	CSAD	XP_001102752.2
Mus musculus (house mouse)	Csad	NP_659191.1
Bos taurus (cattle)	CSAD	XP_005206296.1
Homo sapiens (human)	CSAD	NP_057073.4
Canis lupus familiaris (dog)	CSAD	XP_005636842.1
Rattus norvegicus (Norway rat)	Csad	NP_068518.1
Danio rerio (zebrafish)	csad	NP_001007349.1

Gene Ontology
Bibliography

Related articles in PubMed

Running the Stop Sign: Readthrough of a Premature UAG Termination Signal in the Translation of a Zebrafish (Danio rerio) Taurine Biosynthetic Enzyme.
Larkin MEM, Place AR
Marine drugs15(6)(2017 Jun)

Taurine Biosynthesis in a Fish Liver Cell Line (ZFL) Adapted to a Serum-Free Medium.
Liu CL, Watson AM, Place AR, Jagus R
Marine drugs15(6)(2017 May)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Taurine homeostasis requires de novo synthesis via cysteine sulfinic acid decarboxylase during zebrafish early embryogenesis.
Chang YC, Ding ST, Lee YH, Wang YC, Huang MF, Liu IH
Amino acids44(2)615-29(2013 Feb)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of csad significantly reduced the taurine level and increased embryo mortality and cardiac anomalies.
Title: Taurine homeostasis requires de novo synthesis via cysteine sulfinic acid decarboxylase during zebrafish early embryogenesis.

The following CSAD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CSAD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31285	NM_001007348.1 Latest version!	Danio rerio cysteine sulfinic acid decarboxylase (csad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa27981	XM_009297043.3 Latest version!	Danio rerio cysteine sulfinic acid decarboxylase (csad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa27981	XM_009297043.2	Danio rerio cysteine sulfinic acid decarboxylase (csad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CSAD ( NM_001007348.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001007349.1 Danio rerio cysteine sulfinic acid decarboxylase (csad), mRNA.

Price & Availability↑

CloneID	ODa31285
Clone ID Related Accession (Same CDS sequence)	NM_001007348.1
Accession Version	NM_001007348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	cysteine sulfinic acid decarboxylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085529.1. ##Evidence-Data-START## Transcript exon combination :: BC085529.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGACGAGT CTGATGGGAA GCTGTTCCTT ACTGAGGCTT TCAACATAAT CATGGAAGAA 
ATTCTTAACA AAGGAAGGGA CTTGAAGGAG AAGGTTTGTG AGTGGAAAGA TCCAGATCAG 
CTGAGATCTC TCCTGGACCT CGAACTTCGG GATCATGGAG AATGTCATGA GAAGCTGCTG 
CAGAGGGTTC GAGATGTGGC CAAATACAGC GTAAAAACTT GTCATCCTCG GTTCTTCAAT 
CAGCTGTTTG CTGGCGTGGA CTATCATGCA CTGACAGGAC GGCTCATCAC TGAAACCCTC 
AATACCAGCC AATACACCTA TGAAGTGGCT CCAGTGTTTG TCCTGATGGA GGAGGAAGTG 
ATCAGTAAGC TTCGCTCTCT GGTTGGCTGG TCAGAAGGAG ATGGGATCTT TTGTCCTGGA 
GGATCCATGT CTAACATGTA TGCCATTAAC GTCGCTCGGT ACTGGGCTTT TCCTCAAGTG 
AAGACAAAAG GCTTGTGGGC CGCACCACGG ATGGCTATAT TTACATCACA ACAGAGTCAT 
TACTCCGTGA AAAAAGGAGC TGCGTTTCTT GGTATTGGAA CAGAAAATGT TTTCATTGTG 
CAAGTGGATG AGAGCGGCAG CATGATACCA GAAGACCTGG AGGCAAAAAT TGTGCAGGCA 
AAATCCCAAG ACGCTGTTCC GTTTTTCGTA AACGCCACAG CCGGAACCAC AGTGCAGGGA 
GCCTTTGACC CTCTGAAGCG CATAGCTGAC ATATGTGAAA GAAACGGCAT GTGGATGCAT 
GTTGACGCCG CATGGGGAGG AAGCGTGCTG TTTTCCAAAA AGCACAGACA TCTGGTTGCA 
GGAATAGAAA GAGCAAACTC GGTGACTTGG AATCCTCACA AAATGCTTCT GACGGGACTG 
CAGTGCTCTG TGATTTTGTT CAGAGATACT ACGAATTTGC TCATGCACTG TCACAGTGCC 
AAAGCCACAT ACTTGTTCCA GCAAGACAAG TTCTACGACA CAAGTCTGGA CACGGGCGAC 
AAATCCATCC AGTGTGGCCG GAAGGTGGAT TGCCTCAAGC TCTGGCTCAT GTGGAAGGCA 
ATCGGAGCTA GTGGTCTTTC ACAGCGTGTC GATAAGGCCT TTGCCCTCAC TAGGTATTTA 
GTTGAAGAAA TGGAGAAACG GGAGAATTTC CAGCTGGTCT GTAAGGGGCC GTTTGTGAAC 
GTTTGCTTCT GGTTTATTCC ACCCAGTCTG AAAGGAAAGG AGAACAGCCC AGATTACCAG 
GAAAGACTAT CCAAGGTGGC GCCAGTCATT AAAGAGAGGA TGATGAAGCG AGGAACGATG 
ATGGTGGGAT ATCAGCCAAT GGATGAACAC GTCAACTTCT TCCGCATGGT GGTTGTTTCT 
CCACAGCTCA CAACCAAAGA CATGGATTTC TTCCTTGATG AGATGGAGAA ACTCGGGAAG 
GATCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007349.1
CDS	107..1555
Translation	MDESDGKLFLTEAFNIIMEEILNKGRDLKEKVCEWKDPDQLRSLLDLELRDHGECHEKLLQRVRDVAKYSVKTCHPRFFNQLFAGVDYHALTGRLITETLNTSQYTYEVAPVFVLMEEEVISKLRSLVGWSEGDGIFCPGGSMSNMYAINVARYWAFPQVKTKGLWAAPRMAIFTSQQSHYSVKKGAAFLGIGTENVFIVQVDESGSMIPEDLEAKIVQAKSQDAVPFFVNATAGTTVQGAFDPLKRIADICERNGMWMHVDAAWGGSVLFSKKHRHLVAGIERANSVTWNPHKMLLTGLQCSVILFRDTTNLLMHCHSAKATYLFQQDKFYDTSLDTGDKSIQCGRKVDCLKLWLMWKAIGASGLSQRVDKAFALTRYLVEEMEKRENFQLVCKGPFVNVCFWFIPPSLKGKENSPDYQERLSKVAPVIKERMMKRGTMMVGYQPMDEHVNFFRMVVVSPQLTTKDMDFFLDEMEKLGKDL

Target ORF information:

RefSeq Version	NM_001007348.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cysteine sulfinic acid decarboxylase (csad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007348.1

ORF Insert Sequence:

ATGGACGAGT CTGATGGGAA GCTGTTCCTT ACTGAGGCTT TCAACATAAT CATGGAAGAA 
ATTCTTAACA AAGGAAGGGA CTTGAAGGAG AAGGTTTGTG AGTGGAAAGA TCCAGATCAG 
CTGAGATCTC TCCTGGACCT CGAACTTCGG GATCATGGAG AATGTCATGA GAAGCTGCTG 
CAGAGGGTTC GAGATGTGGC CAAATACAGC GTAAAAACTT GTCATCCTCG GTTCTTCAAT 
CAGCTGTTTG CTGGCGTGGA CTATCATGCA CTGACAGGAC GGCTCATCAC TGAAACCCTC 
AATACCAGCC AATACACCTA TGAAGTGGCT CCAGTGTTTG TCCTGATGGA GGAGGAAGTG 
ATCAGTAAGC TTCGCTCTCT GGTTGGCTGG TCAGAAGGAG ATGGGATCTT TTGTCCTGGA 
GGATCCATGT CTAACATGTA TGCCATTAAC GTCGCTCGGT ACTGGGCTTT TCCTCAAGTG 
AAGACAAAAG GCTTGTGGGC CGCACCACGG ATGGCTATAT TTACATCACA ACAGAGTCAT 
TACTCCGTGA AAAAAGGAGC TGCGTTTCTT GGTATTGGAA CAGAAAATGT TTTCATTGTG 
CAAGTGGATG AGAGCGGCAG CATGATACCA GAAGACCTGG AGGCAAAAAT TGTGCAGGCA 
AAATCCCAAG ACGCTGTTCC GTTTTTCGTA AACGCCACAG CCGGAACCAC AGTGCAGGGA 
GCCTTTGACC CTCTGAAGCG CATAGCTGAC ATATGTGAAA GAAACGGCAT GTGGATGCAT 
GTTGACGCCG CATGGGGAGG AAGCGTGCTG TTTTCCAAAA AGCACAGACA TCTGGTTGCA 
GGAATAGAAA GAGCAAACTC GGTGACTTGG AATCCTCACA AAATGCTTCT GACGGGACTG 
CAGTGCTCTG TGATTTTGTT CAGAGATACT ACGAATTTGC TCATGCACTG TCACAGTGCC 
AAAGCCACAT ACTTGTTCCA GCAAGACAAG TTCTACGACA CAAGTCTGGA CACGGGCGAC 
AAATCCATCC AGTGTGGCCG GAAGGTGGAT TGCCTCAAGC TCTGGCTCAT GTGGAAGGCA 
ATCGGAGCTA GTGGTCTTTC ACAGCGTGTC GATAAGGCCT TTGCCCTCAC TAGGTATTTA 
GTTGAAGAAA TGGAGAAACG GGAGAATTTC CAGCTGGTCT GTAAGGGGCC GTTTGTGAAC 
GTTTGCTTCT GGTTTATTCC ACCCAGTCTG AAAGGAAAGG AGAACAGCCC AGATTACCAG 
GAAAGACTAT CCAAGGTGGC GCCAGTCATT AAAGAGAGGA TGATGAAGCG AGGAACGATG 
ATGGTGGGAT ATCAGCCAAT GGATGAACAC GTCAACTTCT TCCGCATGGT GGTTGTTTCT 
CCACAGCTCA CAACCAAAGA CATGGATTTC TTCCTTGATG AGATGGAGAA ACTCGGGAAG 
GATCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSAD ( XM_009297043.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295318.1 Danio rerio cysteine sulfinic acid decarboxylase (csad), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27981
Clone ID Related Accession (Same CDS sequence)	XM_009297043.3 , XM_009297043.2
Accession Version	XM_009297043.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cysteine sulfinic acid decarboxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAATC GGGCGTGTGC ATCCACGTAT ATGAAGGCTG GACCCTGCAT TAAAGCCCCG 
AGGGTCCCGA GGCATCCTTT ACTTATAGTT GAGTTCCATG TGCTGAGTAC AGAAACGACA 
TTAAACATCA CCATGAGTTC ATCTGGAGAG TATATGAATG GCCACGTCCA CCTGGAAGAA 
TCAGACATGG ACGAGTCTGA TGGGAAGCTG TTCCTTACTG AGGCTTTCAA CATAATCATG 
GAAGAAATTC TTAACAAAGG AAGGGACTTG AAGGAGAAGG TTTGTGAGTG GAAAGATCCA 
GATCAGCTGA GATCTCTCCT GGACCTCGAA CTTCGGGATC ATGGAGAATG TCATGAGAAG 
CTGCTGCAGA GGGTTCGAGA TGTGGCCAAA TACAGCGTAA AAACTTGTCA TCCTCGGTTC 
TTCAATCAGC TGTTTGCTGG CGTGGACTAT CATGCACTGA CAGGACGGCT CATCACTGAA 
ACCCTCAATA CCAGCCAATA CACCTATGAA GTGGCTCCAG TGTTTGTCCT GATGGAGGAG 
GAAGTGATCA GTAAGCTTCG CTCTCTGGTT GGCTGGTCAG AAGGAGATGG GATCTTTTGT 
CCTGGAGGAT CCATGTCTAA CATGTATGCC ATTAACGTCG CTCGGTACTG GGCTTTTCCT 
CAAGTGAAGA CAAAAGGCTT GTGGGCCGCA CCACGGATGG CTATATTTAC ATCACAACAG 
AGTCATTACT CCGTGAAAAA AGGAGCTGCG TTTCTTGGTA TTGGAACAGA AAATGTTTTC 
ATTGTGCAAG TGGATGAGAG CGGCAGCATG ATACCAGAAG ACCTGGAGGC AAAAATTGTG 
CAGGCAAAAT CCCAAGACGC TGTTCCGTTT TTCGTAAACG CCACAGCCGG AACCACAGTG 
CAGGGAGCCT TTGACCCTCT GAAGCGCATA GCTGACATAT GTGAAAGAAA CGGCATGTGG 
ATGCATGTTG ACGCCGCATG GGGAGGAAGC GTGCTGTTTT CCAAAAAGCA CAGACATCTG 
GTTGCAGGAA TAGAAAGAGC AAACTCGGTG ACTTGGAATC CTCACAAAAT GCTTCTGACG 
GGACTGCAGT GCTCTGTGAT TTTGTTCAGA GATACTACGA ATTTGCTCAT GCACTGTCAC 
AGTGCCAAAG CCACATACTT GTTCCAGCAA GACAAGTTCT ACGACACAAG TCTGGACACG 
GGCGACAAAT CCATCCAGTG TGGCCGGAAG GTGGATTGCC TCAAGCTCTG GCTCATGTGG 
AAGGCAATCG GAGCTAGTGG TCTTTCACAG CGTGTCGATA AGGCCTTTGC CCTCACTAGG 
TATTTAGTTG AAGAAATGGA GAAACGGGAG AATTTCCAGC TGGTCTGTAA GGGGCCGTTT 
GTGAACGTTT GCTTCTGGTT TATTCCACCC AGTCTGAAAG GAAAGGAGAA CAGCCCAGAT 
TACCAGGAAA GACTATCCAA GGTGGCGCCA GTCATTAAAG AGAGGATGAT GAAGCGAGGA 
ACGATGATGG TGGGATATCA GCCAATGGAT GAACACGTCA ACTTCTTCCG CATGGTGGTT 
GTTTCTCCAC AGCTCACAAC CAAAGACATG GATTTCTTCC TTGATGAGAT GGAGAAACTC 
GGGAAGGATC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295318.1
CDS	592..2226
Translation	MTNRACASTYMKAGPCIKAPRVPRHPLLIVEFHVLSTETTLNITMSSSGEYMNGHVHLEESDMDESDGKLFLTEAFNIIMEEILNKGRDLKEKVCEWKDPDQLRSLLDLELRDHGECHEKLLQRVRDVAKYSVKTCHPRFFNQLFAGVDYHALTGRLITETLNTSQYTYEVAPVFVLMEEEVISKLRSLVGWSEGDGIFCPGGSMSNMYAINVARYWAFPQVKTKGLWAAPRMAIFTSQQSHYSVKKGAAFLGIGTENVFIVQVDESGSMIPEDLEAKIVQAKSQDAVPFFVNATAGTTVQGAFDPLKRIADICERNGMWMHVDAAWGGSVLFSKKHRHLVAGIERANSVTWNPHKMLLTGLQCSVILFRDTTNLLMHCHSAKATYLFQQDKFYDTSLDTGDKSIQCGRKVDCLKLWLMWKAIGASGLSQRVDKAFALTRYLVEEMEKRENFQLVCKGPFVNVCFWFIPPSLKGKENSPDYQERLSKVAPVIKERMMKRGTMMVGYQPMDEHVNFFRMVVVSPQLTTKDMDFFLDEMEKLGKDL

Target ORF information:

RefSeq Version	XM_009297043.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cysteine sulfinic acid decarboxylase (csad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297043.3

ORF Insert Sequence:

ATGACGAATC GGGCGTGTGC ATCCACGTAT ATGAAGGCTG GACCCTGCAT TAAAGCCCCG 
AGGGTCCCGA GGCATCCTTT ACTTATAGTT GAGTTCCATG TGCTGAGTAC AGAAACGACA 
TTAAACATCA CCATGAGTTC ATCTGGAGAG TATATGAATG GCCACGTCCA CCTGGAAGAA 
TCAGACATGG ACGAGTCTGA TGGGAAGCTG TTCCTTACTG AGGCTTTCAA CATAATCATG 
GAAGAAATTC TTAACAAAGG AAGGGACTTG AAGGAGAAGG TTTGTGAGTG GAAAGATCCA 
GATCAGCTGA GATCTCTCCT GGACCTCGAA CTTCGGGATC ATGGAGAATG TCATGAGAAG 
CTGCTGCAGA GGGTTCGAGA TGTGGCCAAA TACAGCGTAA AAACTTGTCA TCCTCGGTTC 
TTCAATCAGC TGTTTGCTGG CGTGGACTAT CATGCACTGA CAGGACGGCT CATCACTGAA 
ACCCTCAATA CCAGCCAATA CACCTATGAA GTGGCTCCAG TGTTTGTCCT GATGGAGGAG 
GAAGTGATCA GTAAGCTTCG CTCTCTGGTT GGCTGGTCAG AAGGAGATGG GATCTTTTGT 
CCTGGAGGAT CCATGTCTAA CATGTATGCC ATTAACGTCG CTCGGTACTG GGCTTTTCCT 
CAAGTGAAGA CAAAAGGCTT GTGGGCCGCA CCACGGATGG CTATATTTAC ATCACAACAG 
AGTCATTACT CCGTGAAAAA AGGAGCTGCG TTTCTTGGTA TTGGAACAGA AAATGTTTTC 
ATTGTGCAAG TGGATGAGAG CGGCAGCATG ATACCAGAAG ACCTGGAGGC AAAAATTGTG 
CAGGCAAAAT CCCAAGACGC TGTTCCGTTT TTCGTAAACG CCACAGCCGG AACCACAGTG 
CAGGGAGCCT TTGACCCTCT GAAGCGCATA GCTGACATAT GTGAAAGAAA CGGCATGTGG 
ATGCATGTTG ACGCCGCATG GGGAGGAAGC GTGCTGTTTT CCAAAAAGCA CAGACATCTG 
GTTGCAGGAA TAGAAAGAGC AAACTCGGTG ACTTGGAATC CTCACAAAAT GCTTCTGACG 
GGACTGCAGT GCTCTGTGAT TTTGTTCAGA GATACTACGA ATTTGCTCAT GCACTGTCAC 
AGTGCCAAAG CCACATACTT GTTCCAGCAA GACAAGTTCT ACGACACAAG TCTGGACACG 
GGCGACAAAT CCATCCAGTG TGGCCGGAAG GTGGATTGCC TCAAGCTCTG GCTCATGTGG 
AAGGCAATCG GAGCTAGTGG TCTTTCACAG CGTGTCGATA AGGCCTTTGC CCTCACTAGG 
TATTTAGTTG AAGAAATGGA GAAACGGGAG AATTTCCAGC TGGTCTGTAA GGGGCCGTTT 
GTGAACGTTT GCTTCTGGTT TATTCCACCC AGTCTGAAAG GAAAGGAGAA CAGCCCAGAT 
TACCAGGAAA GACTATCCAA GGTGGCGCCA GTCATTAAAG AGAGGATGAT GAAGCGAGGA 
ACGATGATGG TGGGATATCA GCCAATGGAT GAACACGTCA ACTTCTTCCG CATGGTGGTT 
GTTTCTCCAC AGCTCACAAC CAAAGACATG GATTTCTTCC TTGATGAGAT GGAGAAACTC 
GGGAAGGATC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSAD ( XM_009297043.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295318.1 Danio rerio cysteine sulfinic acid decarboxylase (csad), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27981
Clone ID Related Accession (Same CDS sequence)	XM_009297043.3 , XM_009297043.2
Accession Version	XM_009297043.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cysteine sulfinic acid decarboxylase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAATC GGGCGTGTGC ATCCACGTAT ATGAAGGCTG GACCCTGCAT TAAAGCCCCG 
AGGGTCCCGA GGCATCCTTT ACTTATAGTT GAGTTCCATG TGCTGAGTAC AGAAACGACA 
TTAAACATCA CCATGAGTTC ATCTGGAGAG TATATGAATG GCCACGTCCA CCTGGAAGAA 
TCAGACATGG ACGAGTCTGA TGGGAAGCTG TTCCTTACTG AGGCTTTCAA CATAATCATG 
GAAGAAATTC TTAACAAAGG AAGGGACTTG AAGGAGAAGG TTTGTGAGTG GAAAGATCCA 
GATCAGCTGA GATCTCTCCT GGACCTCGAA CTTCGGGATC ATGGAGAATG TCATGAGAAG 
CTGCTGCAGA GGGTTCGAGA TGTGGCCAAA TACAGCGTAA AAACTTGTCA TCCTCGGTTC 
TTCAATCAGC TGTTTGCTGG CGTGGACTAT CATGCACTGA CAGGACGGCT CATCACTGAA 
ACCCTCAATA CCAGCCAATA CACCTATGAA GTGGCTCCAG TGTTTGTCCT GATGGAGGAG 
GAAGTGATCA GTAAGCTTCG CTCTCTGGTT GGCTGGTCAG AAGGAGATGG GATCTTTTGT 
CCTGGAGGAT CCATGTCTAA CATGTATGCC ATTAACGTCG CTCGGTACTG GGCTTTTCCT 
CAAGTGAAGA CAAAAGGCTT GTGGGCCGCA CCACGGATGG CTATATTTAC ATCACAACAG 
AGTCATTACT CCGTGAAAAA AGGAGCTGCG TTTCTTGGTA TTGGAACAGA AAATGTTTTC 
ATTGTGCAAG TGGATGAGAG CGGCAGCATG ATACCAGAAG ACCTGGAGGC AAAAATTGTG 
CAGGCAAAAT CCCAAGACGC TGTTCCGTTT TTCGTAAACG CCACAGCCGG AACCACAGTG 
CAGGGAGCCT TTGACCCTCT GAAGCGCATA GCTGACATAT GTGAAAGAAA CGGCATGTGG 
ATGCATGTTG ACGCCGCATG GGGAGGAAGC GTGCTGTTTT CCAAAAAGCA CAGACATCTG 
GTTGCAGGAA TAGAAAGAGC AAACTCGGTG ACTTGGAATC CTCACAAAAT GCTTCTGACG 
GGACTGCAGT GCTCTGTGAT TTTGTTCAGA GATACTACGA ATTTGCTCAT GCACTGTCAC 
AGTGCCAAAG CCACATACTT GTTCCAGCAA GACAAGTTCT ACGACACAAG TCTGGACACG 
GGCGACAAAT CCATCCAGTG TGGCCGGAAG GTGGATTGCC TCAAGCTCTG GCTCATGTGG 
AAGGCAATCG GAGCTAGTGG TCTTTCACAG CGTGTCGATA AGGCCTTTGC CCTCACTAGG 
TATTTAGTTG AAGAAATGGA GAAACGGGAG AATTTCCAGC TGGTCTGTAA GGGGCCGTTT 
GTGAACGTTT GCTTCTGGTT TATTCCACCC AGTCTGAAAG GAAAGGAGAA CAGCCCAGAT 
TACCAGGAAA GACTATCCAA GGTGGCGCCA GTCATTAAAG AGAGGATGAT GAAGCGAGGA 
ACGATGATGG TGGGATATCA GCCAATGGAT GAACACGTCA ACTTCTTCCG CATGGTGGTT 
GTTTCTCCAC AGCTCACAAC CAAAGACATG GATTTCTTCC TTGATGAGAT GGAGAAACTC 
GGGAAGGATC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295318.1
CDS	1852..3486
Translation	MTNRACASTYMKAGPCIKAPRVPRHPLLIVEFHVLSTETTLNITMSSSGEYMNGHVHLEESDMDESDGKLFLTEAFNIIMEEILNKGRDLKEKVCEWKDPDQLRSLLDLELRDHGECHEKLLQRVRDVAKYSVKTCHPRFFNQLFAGVDYHALTGRLITETLNTSQYTYEVAPVFVLMEEEVISKLRSLVGWSEGDGIFCPGGSMSNMYAINVARYWAFPQVKTKGLWAAPRMAIFTSQQSHYSVKKGAAFLGIGTENVFIVQVDESGSMIPEDLEAKIVQAKSQDAVPFFVNATAGTTVQGAFDPLKRIADICERNGMWMHVDAAWGGSVLFSKKHRHLVAGIERANSVTWNPHKMLLTGLQCSVILFRDTTNLLMHCHSAKATYLFQQDKFYDTSLDTGDKSIQCGRKVDCLKLWLMWKAIGASGLSQRVDKAFALTRYLVEEMEKRENFQLVCKGPFVNVCFWFIPPSLKGKENSPDYQERLSKVAPVIKERMMKRGTMMVGYQPMDEHVNFFRMVVVSPQLTTKDMDFFLDEMEKLGKDL

Target ORF information:

RefSeq Version	XM_009297043.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cysteine sulfinic acid decarboxylase (csad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297043.2

ORF Insert Sequence:

ATGACGAATC GGGCGTGTGC ATCCACGTAT ATGAAGGCTG GACCCTGCAT TAAAGCCCCG 
AGGGTCCCGA GGCATCCTTT ACTTATAGTT GAGTTCCATG TGCTGAGTAC AGAAACGACA 
TTAAACATCA CCATGAGTTC ATCTGGAGAG TATATGAATG GCCACGTCCA CCTGGAAGAA 
TCAGACATGG ACGAGTCTGA TGGGAAGCTG TTCCTTACTG AGGCTTTCAA CATAATCATG 
GAAGAAATTC TTAACAAAGG AAGGGACTTG AAGGAGAAGG TTTGTGAGTG GAAAGATCCA 
GATCAGCTGA GATCTCTCCT GGACCTCGAA CTTCGGGATC ATGGAGAATG TCATGAGAAG 
CTGCTGCAGA GGGTTCGAGA TGTGGCCAAA TACAGCGTAA AAACTTGTCA TCCTCGGTTC 
TTCAATCAGC TGTTTGCTGG CGTGGACTAT CATGCACTGA CAGGACGGCT CATCACTGAA 
ACCCTCAATA CCAGCCAATA CACCTATGAA GTGGCTCCAG TGTTTGTCCT GATGGAGGAG 
GAAGTGATCA GTAAGCTTCG CTCTCTGGTT GGCTGGTCAG AAGGAGATGG GATCTTTTGT 
CCTGGAGGAT CCATGTCTAA CATGTATGCC ATTAACGTCG CTCGGTACTG GGCTTTTCCT 
CAAGTGAAGA CAAAAGGCTT GTGGGCCGCA CCACGGATGG CTATATTTAC ATCACAACAG 
AGTCATTACT CCGTGAAAAA AGGAGCTGCG TTTCTTGGTA TTGGAACAGA AAATGTTTTC 
ATTGTGCAAG TGGATGAGAG CGGCAGCATG ATACCAGAAG ACCTGGAGGC AAAAATTGTG 
CAGGCAAAAT CCCAAGACGC TGTTCCGTTT TTCGTAAACG CCACAGCCGG AACCACAGTG 
CAGGGAGCCT TTGACCCTCT GAAGCGCATA GCTGACATAT GTGAAAGAAA CGGCATGTGG 
ATGCATGTTG ACGCCGCATG GGGAGGAAGC GTGCTGTTTT CCAAAAAGCA CAGACATCTG 
GTTGCAGGAA TAGAAAGAGC AAACTCGGTG ACTTGGAATC CTCACAAAAT GCTTCTGACG 
GGACTGCAGT GCTCTGTGAT TTTGTTCAGA GATACTACGA ATTTGCTCAT GCACTGTCAC 
AGTGCCAAAG CCACATACTT GTTCCAGCAA GACAAGTTCT ACGACACAAG TCTGGACACG 
GGCGACAAAT CCATCCAGTG TGGCCGGAAG GTGGATTGCC TCAAGCTCTG GCTCATGTGG 
AAGGCAATCG GAGCTAGTGG TCTTTCACAG CGTGTCGATA AGGCCTTTGC CCTCACTAGG 
TATTTAGTTG AAGAAATGGA GAAACGGGAG AATTTCCAGC TGGTCTGTAA GGGGCCGTTT 
GTGAACGTTT GCTTCTGGTT TATTCCACCC AGTCTGAAAG GAAAGGAGAA CAGCCCAGAT 
TACCAGGAAA GACTATCCAA GGTGGCGCCA GTCATTAAAG AGAGGATGAT GAAGCGAGGA 
ACGATGATGG TGGGATATCA GCCAATGGAT GAACACGTCA ACTTCTTCCG CATGGTGGTT 
GTTTCTCCAC AGCTCACAAC CAAAGACATG GATTTCTTCC TTGATGAGAT GGAGAAACTC 
GGGAAGGATC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Running the Stop Sign: Readthrough of a Premature UAG Termination Signal in the Translation of a Zebrafish (Danio rerio) Taurine Biosynthetic Enzyme.
Marine drugs15(6)(2017 Jun)
Larkin MEM,Place AR

Taurine Biosynthesis in a Fish Liver Cell Line (ZFL) Adapted to a Serum-Free Medium.
Marine drugs15(6)(2017 May)
Liu CL,Watson AM,Place AR,Jagus R

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Taurine homeostasis requires de novo synthesis via cysteine sulfinic acid decarboxylase during zebrafish early embryogenesis.
Amino acids44(2)615-29(2013 Feb)
Chang YC,Ding ST,Lee YH,Wang YC,Huang MF,Liu IH

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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CSAD cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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