frem2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	frem2a
Entrez Gene ID	555681
Full Name	Fras1 related extracellular matrix protein 2a
Synonyms	bla
General protein information	Preferred Names Fras1 related extracellular matrix protein 2a Names FRAS1-related extracellular matrix protein 2 Fras-related extracellular matrix protein 2a blasen
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001137657.1	NP_001131129.1	FRAS1-related extracellular matrix protein 2 precursor
XM_005155380.3	XP_005155437.1	FRAS1-related extracellular matrix protein 2 isoform X1
XM_005155380.4	XP_005155437.1	FRAS1-related extracellular matrix protein 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	FREM2	NP_997244.3
Danio rerio (zebrafish)	frem2b	NP_001131133.1
Pan troglodytes (chimpanzee)	FREM2	XP_522664.2
	FREM2	XP_001086486.1
Bos taurus (cattle)	FREM2	XP_002691845.1
Canis lupus familiaris (dog)	FREM2	XP_005635476.1
Rattus norvegicus (Norway rat)	Frem2	NP_001232907.1
Mus musculus (house mouse)	Frem2	NP_766450.2
Gallus gallus (chicken)	FREM2	XP_417087.3
Danio rerio (zebrafish)	frem2a	NP_001131129.1
Xenopus tropicalis (tropical clawed frog)	LOC100496696	XP_004912033.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages.
Nagendran M, Arora P, Gori P, Mulay A, Ray S, Jacob T, Sonawane M
Development (Cambridge, England)142(2)320-30(2015 Jan)

AMACO is a component of the basement membrane-associated Fraser complex.
Richardson RJ, Gebauer JM, Zhang JL, Kobbe B, Keene DR, Karlsen KR, Richetti S, Wohl AP, Sengle G, Neiss WF, Paulsson M, Hammerschmidt M, Wagener R
The Journal of investigative dermatology134(5)1313-1322(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The fish gene complement includes fras1, frem1a, frem1b, frem2a, frem2b, and frem3, which display complex overlapping and complementary expression patterns in developing tissues including the pharyngeal arches, hypochord, musculature, and otic vesicle.
Title: Expression of the fras1/frem gene family during zebrafish development and fin morphogenesis.

The following frem2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the frem2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33784	NM_001137657.1 Latest version!	Danio rerio FRAS1 related extracellular matrix 2a (frem2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa16167	XM_005155380.4 Latest version!	Danio rerio Fras1 related extracellular matrix protein 2a (frem2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
ODa16167	XM_005155380.3	Danio rerio Fras1 related extracellular matrix protein 2a (frem2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

frem2a ( NM_001137657.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001131129.1 Danio rerio FRAS1 related extracellular matrix 2a (frem2a), mRNA.

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CloneID	ODa33784
Clone ID Related Accession (Same CDS sequence)	NM_001137657.1
Accession Version	NM_001137657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Danio rerio(zebrafish)
Product	FRAS1-related extracellular matrix protein 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BK006471.1. On Nov 14, 2008 this sequence version replaced XM_678271.2. ##Evidence-Data-START## Transcript exon combination :: BK006471.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA4476757, SAMEA4476810 [ECO:0000350] ##Evidence-Data-END##

ATGGAACTTC ACAGATTTCT TCTCCTCCTG CTATCACTGA CAGGCAGTTC CATGAGCGCA 
CTTGAAACGC ACGGACTGTC GTACGCGTCC AAGCCTGAAC TCTCCGAGGA CGCTATACTG 
GTGGCAAACA ACGGCATACA TGTGCCTTTC GGGAGATCTG TTTACATAGA TCCGATCAAC 
GACTTGGTTA TTGAACTTCA GCCGGGAGAC AGGTGCACTA TAACAGTTCT GGATAATGAT 
CCGCTGTCTC AGAGACCGGG ACACGTGTCT CCTAAAAAGT TCCCGTGCGA ATTCGGTCCT 
AACGATGTGA GATACTCTCA TTTCGGTTCT AGGAGCCCTG CAGGGGACAG AGTGAAGCTG 
CAACTCAGGT ATGACACACA GACTGAAACT ATCATCATCC CCTTTATGCT GGAAGTGGAG 
GTTGTTTTCA CACAATTGGA GGTTGTTACT AAGAACATGC CTCTGGTTGT TGATAAACTA 
CATGGAACTA GTAATCCTAT TAATAAAAAG ATTTTAGATT TCACTTATGA AAAAGACTCT 
GAACAATGTG AGGTAACGTC ACTGGCTGCC AGCAGTGTTC TTCCTAAATA CGGTAAAGTT 
GTTGATGATA AAAAGCTGGA TAAAATGATG AGCTGTGAGT TGTTCCTCAA ATCAAATATC 
CGTTATGAGC ACACGTCTAG CCACAAGTCC CCTAATAAGG ATTACATCCC CATGGTTGTG 
GAGCTACGAA ACAAAGAAAA TAATTTGCTG AAACAGGAGT TTTTCCATGT GATGATCCGG 
ATCAAAGAGG GTGAAGAAAA CACTCCCCCT AGACCCAGTT TTGTGGCTAT GATGATGATG 
GAAGTTGATC AGTTTGTAAT GACGGCTTTA ACTCCTGATA TGTTGGCTGC TGAGGACACT 
GAATCCAATT CTGATGATCT CATTTTCAAC ATCACTTCTC CTCTGTCCAT TGAGGAGGGT 
TATATCGTGA GTACAGATGA CAGGAATTTA CCGATTACAT CCTTTTATCA AAAGGATCTG 
AAAGATCTGA AAATAGCATA CAAACCCCCC TCTTTGGACT CAGACACTGA GCGTATCTTT 
CAAATAGAGT TTGAGGTCAT CGACCCAGAT GGTGGCATTT CAGATCCTTT TGCTTTCATG 
ATCGTTGTGA AGCCCATGAA TACATTAGCG CCGATAGTCA CTAAAAATTC TGGCCAGCTT 
TTATATGAAG GCCAGTCTAG GTCTTTGTTT ACTCAAAACA ATTTAGAAAT TAGCGACGAA 
GACAACCTGG ACAGTGTGAG AATAACAGTG GTTGATGGGC TGCGTCATGG TGTCCTAACT 
GTTTTGGGCT CCAACAGAAA GTATTTTTCT CCGGCTGACT TGGAGGTTGG CATTGTGGTT 
TACCAGCATG ATGGCAGTGA CACATACAGT GATAATATAA TATTCAGAAT GACAGACGGG 
AAGAATGAAG TGGAGTTTTT GTTCCCCATC ACAATAGTAC CCACAGACGA TGAACCACCC 
ATCATAAATG CAAACACTGG ACTGATATTA TTCAAAAATC AGATGGTGCC CATCTCGCAG 
CTCATGCTCA GTGCTGCAGA TATTGACTCT GAAGATTCCA TTATTAAATT TACAATCGAG 
CCTCCCTTTT CAGCTATTGG CGAGGTCGTG TTGAGGCAAT CTGAAGCACC AGAGGATCCG 
TCATCCTGGA AATTTAATGC AGACGACGGT GTGTATGAGA AAGTAGTGAC AGAGTGGCTC 
CAGAAAGATA TTACAAATGA AAAGCTCTTC TATAGGCATA CGGGTCCACA TATCACAGAG 
ACTGTTACAG ATCAGTTTGT GTTTAAAGTG AAAGATGACG CTGATCCTCC CAACCAGTCC 
AGTGAGAATA ATTTTATTAT TAAAATTTTG CCTATTGATG ATATTCCACC CGTGCTGTTC 
CCAGGCACTA CTTTACAGAT GACAGTACAT GAGTACCAGC TGACTCATTT AAGCAAGAAT 
GTTCTTCGTT ACACCGACTT GGACTCAGAG GACCGGGACC TGAAATACAC AATAACTCAA 
GCACCAACTG ATATGGATGA AAACAATCCT GTCAAATTTG GCTCTATTGT TTTAACTGAC 
AGCCCCGATA CTGATGTAAC CGAATTTACC CAAGCGCAAA TTAACCATCA TAAGATTTCC 
TACAACCCGC CAGACCTTGA ACTCGGCATC ATACCTCATG TGGTGCAGTT CCATTTCACA 
GTAGAGGACA CTGCTGGAAA TGGTGCTGAT GGAGTTTTTA CAATCTTTCT GCAGCCTGTA 
AACAACAAAC CCCCACAGAT AACAAACTCT GGATTCACAG TCCTTGAGAG AGGGGTGCAT 
GTTATTACGA GCGCCGAACT TGACGCCAAT GACCTTGATA CTGACATTAA GCTAATATCA 
TTCACGCTAA GTCAAACTCC ACTGCACGGT CATCTTCAAC ATACGTTTAC TGACTTAAAA 
AAAGGGAGTC ATTTCAACTT GATGGATATT TCTGATGGTC AGGTGTCATA TGTCCACAAT 
GGGGATGAAA CGACAAGTGA CTCTTTTAAA CTAGATGTTA GTGACGGTGT TCATGTTGTA 
ACGATCACTG TGAAGGTAAC TGTAAAGCTT ATTGATGATG AAACACCAAC ACTTAGCCTC 
ACAAAGGGCA CCTTTGGCTC TAGATTGGAT GTGCTGGAAA ATGGGGCTAC AGAAATTACT 
CCCAATGTCA TTCAGGGGAA AGATGAGGAC ACTGATGACC TGCAGTTGAC ATTCATTGTG 
GAAGAAGCAC CTAAGTTTGG AGAAATTCTG GTGAATGGAG TTTCCTCGAG CAGATTCACC 
CAAGCTGACA TCATCAGTGG TCTTGTTGTG TATGCGCACA CCAGTGGTGA GATTGGTCTT 
ACTTCAATTG AAGATTTTTT CAGTTTGACC CTCTCTGACA TGTCTGATGA GTGGAATGTA 
GGGGGTAACC GAGTACACGG AGTCCGTGTT CATGTCACCA TTCTCCCTGT AGATAACCAG 
ACTCCATTGG TCACAGTTGG CCCTACTTTT AATGTCTTTG AAGCAGATAA AAACATTATC 
GGGAGCCATC AGATTAAAGT CGAGGATGCC GACACACCAA ACGAGGACAT CCTGTGCACC 
ATTATCGTTC AGCCCACATC AGGATATGTG GAGAACATTT CTCCAGCTCC AGGCTCTGAA 
AAGTCAAGAG CAGGAACGGC AGTGAGTGCT TTCACCATCA GTGATGTGAA TGAAGGTCAT 
ATTTATTATG TCCAAAGCAT ACACAAAGGA GTTGAGCCAG TGGAGGACAG GTTCACGTTC 
AGATGTTCTG ATGGTGTGAA TTTCTCAGAG AGACAGTTCT TTCCCATTGT CATTATCCCC 
ACAAATGATG AAAAACCAGA AATCTTCATG CGAGAGGTTG TTGTAATGGA GGGTATGAAT 
GTTATTATTG ACACGCCACT CTTGAATGGA GTCGATGCTG ATGTTCCTGC TCAAGAGTTG 
ATGTTTATTA TCACCAAACT TCCCAATCAT GGGTTCATTC TCAATCAGCT CGCCAGTGGC 
TCTGTCCCAG TCACAAACTT CACTCTGGAA CAAATCAAAG AAGCCTCTAG CATAATTTAC 
GAACATGATG ATTCTGAAAC CACCGAAGAC AAGTTTGATG TTCTTCTTAC AGATGGGAAA 
CATAAAGCTG AAGGCACAGT CATGATCATG ATTATTCCAG TGGATGATGA AACACCAAGG 
CTTGCCATAA ATGACGGGCT TGAAATTGAG ATTGGTGAAA CAAAAGAGAT AAATGCTAAA 
GTGCTGAAAG CTACTGATTT GGACTCAGAC GACGAGTCCC TTACTTTCAT TATTCGCTAC 
GGCCCAGCTC AAGGCTTACT GTATAGAAAA TCAGCAGGAT CTGTGGAAAA GAGATCTGCT 
GGATCTACAG AGAACATAAC AGTTGGCATG AACTTTACAC AAAATGAGGT GAACAGAGGG 
CTCATTTTGT ACACACACAA TGGACAGGAA GGCATGCGTG ATCTTGTAAA GTTTGATGTA 
ACTGACGGCA TCAATCCCCT AATTGACAGA TACTTCTATA TCACTGTGGG CAGCATTGAC 
ATGGTGTTTC CTGATGTCAT CAGTAAGGGG GTGTCTCTTA AAGAAGGTGG CAAAGTGACA 
CTCACTACGA ACCTCCTCAG CACTAGTGAT CTCAACAGCC CCGATGAGAA TTTAGTCTTT 
ACTATCACAA GAGCCCCAGT GAGAGGACAT TTGGAAAGCA CGGACACACC AGGCATGCCT 
ATAGTATCCT TTACCCAATT ACAACTGGCC GGGAGCAAGA TATACTACAT CCATACTGCT 
GATGATGAAG TCAAGATGGA CAGCTTTGAG TTTGAGGTGA CCGATGGTTA CAACCCTGTG 
TTTCGCACAT TTCGCGTTTC AATCGTTGAT GTTGACAATA AAAAGCCAGT ATTGACCATT 
CATGACCTTC TAGTAACGGA GGGACAAACC AAACTCATTA CACCCTTTGA GTTGACCGTT 
GAGGACCAAG ACACTGCCGA GCACCTTCTG AAGTTTAAAG TCTCCCAATT GCCCATTCAT 
GGGAAACTAC TTTTCAACAA CAGCCAGCCA TTGACTTCTT TCACAAAGCA AGACCTAAAT 
GAAAACATGA TCAGTTACAA GCATGATGGA ACGGAGTCGA AAAAAGATAG CTTTTCATTT 
ACAGTCACCG ATGGTACCCA TTCAGACTTT TTTGTATTCC CTGATACAAT TTTTGAGACC 
CAAAGGCCTC AGACCATGAA GATCAACATT GTGTCTGTGG ACAATGGCGT GCCTCAGATT 
GAGGTTAACA AAGGTGCACC AACTTTAAAA GTATTGTTCG CTGGACACTT GGGTTATCAG 
ATGACAAGCA AAGTCCTGAA GGCAGTGGAC AGAGACAGCA ACCCAGAGGC TTTAATTTTT 
CACATTACCA CACAGCCAGA GCATGGTTAT CTCATTAGCC TGGACAGAGG GACGGAGAAT 
GACTCTATCA ACACCTTCAC ACAAGCTGAC ATAAATGATC TTAAGATCTG CTATATCCTG 
AAGGACGAGA ACAATGCCAC CAGTGACGTC TTTAGTTTTA CCGTGGAGGA TAATGGAGGG 
AACAAACTGA AGAACCAACC GTTTCATCTG AACTGGTCTT GGATCTCTCT GGAGAAGCCA 
AACTATGTGG TGGATGAGCA CGACAAAGTT CTGGAGGTGA CCCTCAGACG GAGAGGTTTC 
CTGGGTGAAA CGTCTTTTGT TGGGATTGGT ACCTTGGATG GCAGTGCAGA GAAGGAGAAG 
GATTTCCTGG GAAAATCGCA GAAGCAGGTG CAGTTTAACC CTGGCCAGAC CACAGCCTCC 
TGGAAAGTCA GAGTTCTCTC GGATGGGAAA TACGAGCAGT CCGAAACGTT TCAAATTGTG 
CTGACTGAGC CGGTGATGGG CGTTTTGGAG GCGCCAGCTG TAGCCACAGT TGAGATTGTG 
GATCCAGGTG ATGAGTCCAT GGTGTTCTTT CCTCAGGCCG GCTTTGCTGT GGAAGAAGAT 
ATTGGAGAGC TGCTTGTCCC AGTGCGCAGG ACTGGAGACG TGAGCCAGGA GCTCATGGTG 
GTCTGTCACA CACAGCAAGA CTCTGCCACA GGAACTGTCC CCACATCGGT TCTGTCCTAC 
TCAGACTACA TCTCGCGTCC AGAGGAGCAG GTCAGCATCC TGCGCTTTGA TAAGGACGAG 
ACGGAAAAAC ACTGTCGTGT GGTGATCATC GATGACTCTC TGTATGAGAG CGAGGAGAGC 
TTCAACGTGA CCCTCAGCAT GCCTATAGGA GGCCGTCTGG GGCCCGAATA CCCCACTGTG 
AATATCCGCA TTCTGCCCGA CCAGGATGAT GCTCCGGTGT TCTACTTTGG CAGTCAGGAG 
TATCATGTGG ATGAGAGTGA CGGTCATGTT GAAGTGCGTG TGTGGAGAAC GGGCACAGAT 
CTCTCTAAAA CGGCAACGGT CACTGTGAGA TCCCGCAAAA CAGACCCTGT TTCTGCTGAA 
GCTGGAGTGG ACTATGTGGG AATCAGCAGA AACTTAGACT TCGCTCCGGG TGTAAGCATG 
CAGACGGTGC GGGTCACGGT GCTGGATGAC CTCGGACAGC CTGTTCTGGA AGGGACAGAA 
AGATTTGAGC TTGTTCTCCG GATGCCCTCC AATGGAATTC TTGGAGAGCC GGCCAAAGTC 
ACAGTCTTTA TCAACGACTC CATTTCAGAC CTGCCAAAGG TGCAGTTTAA ACAGGCTGTC 
GTGACTGTAG AGGAGAGTGA AGGTCATGTC ACTGTAATGG TGCACCGCAG TGGAGACATT 
AAACACAAGT CCAGTGTCCG CTGCTACACG CGGCAGGGCT CCGCACAGGT GGTATACGAC 
TTTGAGGAGA GACCAAACAC TGACGCCTCC ATCGTAACTT TCTTGCCTGG TGAAGTTGAT 
AAACCTTGTG TCTTGAAACT GGTGGATGAT TCAATTCATG AGGAGGAGGA AGAACTGAGG 
CTCGTTTTGG GAAATGCAAA GAGTGATTCG CCATACGGAG CGTCCATTGG AAACCAGAAT 
GAAACACTCA TAAAGATCAA GGACAATGCT GACAAATCCA TCATAAAGTT TGCCGAGACG 
AAGTTCAGTG TTAGCGAACC GAAAGAGTCT GGGCAGGTGA CATTAGTAAA GATCCCCATC 
CGCAGAACAG GAGACTCTTC CAAAGTGTCC CTAGTGAGGG TGCACACTAA AGACGGCTCT 
GCCATATCCG GAGAAGACTA TCATCCTATC TCCCTCGATG TGGAGTTTAA AGCAGAAGAG 
ATCGAGCATG TAGTTGAAGT GGAGGTTTTG TTTGATGGAA TAAGAGAGAT GAGAGAGGCC 
TTCACTGTCC ATTTAAAACC AGATGAGAAC ATGATTGCTG AAATACAGAT GACAAAAGCA 
ATAGTGTACA TCGAGGAGAC CAACAGCATG GCTGATGTCA CATTCCCTTC ATTGCCACAT 
ATTGTGTCAC TGCTGCACTA TGACAATATC AGAACAAGCA AACAAAGTGC TCATCCACCC 
GCTGGATATC CTGTCGTCTG TGTCACGGCC TGCAACCCCA AATATCCAGA CTACGACAAA 
ACAGGCTCCA TCTGCATTAG CGAACGCATC AACAACACGC TGACTCGTTA CCGATGGCTC 
ATTAGCGCCC CCAGCGGTCC TGATGGCGTC ACCAGCCCCA TGAGGGAGGC TGACTTCGAC 
ACCTTCTTCA CATCCTCCAA AATGATCACC CTAGACTCCA TTTACTTCCA AGCGGGCTCC 
AGAGTGCAGT GTGCAGCCCG TGCCGTGAAT TCAAATGGAG ACGAGGGTTT GGAACTGAGC 
AGCCCAATAG TGTCTATTAG CATGGAGGAA GGGATGTGCC AACCACGAAA ATTGGGCACA 
GTGGGTGCTG AGCCCTTTTC TGCCAAACTA CGTTATACGG GCGCTGAAGA CCTTGAATAT 
GCCAATCTCA TCAAGCTTAC TGTCACCATG CCCCACATAG ACGGCATGCT CCCCGTGGTC 
TCCACTCGTG CTCTGTCCAA TTTTGACCTG ACTCTGAGCC CCGATGGCAC TCGCGTGGGA 
AACCACAGGT GCTCCAATCT GCTGGACTTT AATGAGGTGT CCACACGGCA CGGCTTCCTG 
ACCAAAGCCA CCCGCAATAT CCAGAGCGTT GGAGAGACCC TACCTTATCA GTACAGCCCT 
GTCCTCCGAG GCCAGGGCAC TTTACGCTTC TACAGGAACC TGAACCTGGA GGCCTGTCTG 
TGGGAGTTTG TGAGTTATTA TGACATGTCA GAGCTGCTCA CAGACTGCGG GGGAACCATC 
GGGACAGATG GCCAGGTGCT GAACCTGGTG CAGTCATACG TGACCCTGCG TGTTCCTCTT 
CACGTCTCAT ACGTGTTTCA TTCCCCTGTG GGTGCTGGAG GCTGGCAGCA TTTCGACCTG 
CACTCCGAGC TCCGGCTCAC CTTTGTTTAT GACACTGCCA TCTTGTGGAA GGACGGAGTC 
GGCAGCCAAA CACAGGCTGA TCTACAAGGC AACCTTGGAG CTTTATATCC CACCAGCATG 
AGGATTAACG AGCAGGGGAG ACTCGTGGTC AACTTCCGCA CAGAGGCCCG CTTCAGGGGT 
TTATTTGTAG TGGGGCATCC TGCGTCCACA GTGTCTACAG TGATGAGTGC AGATCACCCA 
GGCTTGACCT TCAACCTAAC TCTGGTCAGG ACGGAGCCGA CCTACAGCCA ACCCGTCCAG 
CAGTGGATGT TTATCTCAGA CTTCGCTGTT CGTGATTATT CTGGCACATA CACAGTGAGC 
TTGATCCCCT GTACAGCTGC TCTGAATGTG GAGTTCACAG TTCCTCCAGT CTGCAACCCT 
AGAGAAGCCA TCACGTTTGA TCTCGATATC CGCTTCCAGC AGGTGAGTGA CCCTGTGGCT 
GTGGAGTTCA GTCTGAACAC ACAGATGTTC CTGCTGTCTA AGAAAGTGCT GTGGCTGTCA 
GATGGATCAA TGGGCTTTGG TCAGGAAAGT GACGCCGCTT TCTCTGAAGG TGACGTGATC 
TATGGACGTG TGATGGTGGA CCCGGTACAG AATTTGGGCG ACTCTTTCTA CTGTAACATT 
GAGAAGGTGT ATCTGTGCAC TGGAGCTGAT GGATATGTTC CTAAATACAA CCCCAGCAAG 
TCTGAGTTTG GATGCCTTGC TGACTCTCCG TCTCTGCTCT ACCAATTCAA GATTATTGAC 
AAAGCTCAGC CAGAAACTCA GGCCAGAAGT TTTGGGAATG TGGACTTCAA TGCTCTGCTG 
GCTGTAGATG ACCCATCGGC CCTCTCTCTG GTCAAACAGC CTGGATCTGA TGGCTTCAGA 
CTCCATTCTA CACCCCTCTT CCAGGTGGCA GTAGGGAGAG AGTGGTTCAT TCACACCATC 
TACACTGTGC GTTCAAAAGA CAACGCCAAA CGTGGCATCG GCAAACGAAG TGTCGAACAG 
CACTTGATAA CATCAGGAGA CCTTAAAGAG TCAAGAAGAC GCTCCAGACG CTCCAACAGA 
GACGTTCCCA CACTGGCTGA GCAGATTGGC CCCGAAAACA ATCGAGGAAC CAACATCATG 
CACATCGCTC TAGACCGCAG CCGCCGCACT GCATCTGAAG AAGAGCTGTT TGCGGAGGGA 
CTTTCTCCAC GAGGACTCAG TGGACGACAG GCAGAAGAAG AAGCGGTGAC GGTGGCAGGA 
GCTCTTGTGG GTTTGCTGCT CACCGTTCTT GTGTTCGTAG CATTAGCATT GGTGGTTCGC 
TCAAGGCGGA CGCCAGCTAA AAAGGCCCCA AGAGTATCAC CCAGCCTGCA GCCGGTCATT 
GTGCGGAGCA GTCTTGATAA TGGGGACAGT TCAGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001131129.1
CDS	1..9342
Translation	MELHRFLLLLLSLTGSSMSALETHGLSYASKPELSEDAILVANNGIHVPFGRSVYIDPINDLVIELQPGDRCTITVLDNDPLSQRPGHVSPKKFPCEFGPNDVRYSHFGSRSPAGDRVKLQLRYDTQTETIIIPFMLEVEVVFTQLEVVTKNMPLVVDKLHGTSNPINKKILDFTYEKDSEQCEVTSLAASSVLPKYGKVVDDKKLDKMMSCELFLKSNIRYEHTSSHKSPNKDYIPMVVELRNKENNLLKQEFFHVMIRIKEGEENTPPRPSFVAMMMMEVDQFVMTALTPDMLAAEDTESNSDDLIFNITSPLSIEEGYIVSTDDRNLPITSFYQKDLKDLKIAYKPPSLDSDTERIFQIEFEVIDPDGGISDPFAFMIVVKPMNTLAPIVTKNSGQLLYEGQSRSLFTQNNLEISDEDNLDSVRITVVDGLRHGVLTVLGSNRKYFSPADLEVGIVVYQHDGSDTYSDNIIFRMTDGKNEVEFLFPITIVPTDDEPPIINANTGLILFKNQMVPISQLMLSAADIDSEDSIIKFTIEPPFSAIGEVVLRQSEAPEDPSSWKFNADDGVYEKVVTEWLQKDITNEKLFYRHTGPHITETVTDQFVFKVKDDADPPNQSSENNFIIKILPIDDIPPVLFPGTTLQMTVHEYQLTHLSKNVLRYTDLDSEDRDLKYTITQAPTDMDENNPVKFGSIVLTDSPDTDVTEFTQAQINHHKISYNPPDLELGIIPHVVQFHFTVEDTAGNGADGVFTIFLQPVNNKPPQITNSGFTVLERGVHVITSAELDANDLDTDIKLISFTLSQTPLHGHLQHTFTDLKKGSHFNLMDISDGQVSYVHNGDETTSDSFKLDVSDGVHVVTITVKVTVKLIDDETPTLSLTKGTFGSRLDVLENGATEITPNVIQGKDEDTDDLQLTFIVEEAPKFGEILVNGVSSSRFTQADIISGLVVYAHTSGEIGLTSIEDFFSLTLSDMSDEWNVGGNRVHGVRVHVTILPVDNQTPLVTVGPTFNVFEADKNIIGSHQIKVEDADTPNEDILCTIIVQPTSGYVENISPAPGSEKSRAGTAVSAFTISDVNEGHIYYVQSIHKGVEPVEDRFTFRCSDGVNFSERQFFPIVIIPTNDEKPEIFMREVVVMEGMNVIIDTPLLNGVDADVPAQELMFIITKLPNHGFILNQLASGSVPVTNFTLEQIKEASSIIYEHDDSETTEDKFDVLLTDGKHKAEGTVMIMIIPVDDETPRLAINDGLEIEIGETKEINAKVLKATDLDSDDESLTFIIRYGPAQGLLYRKSAGSVEKRSAGSTENITVGMNFTQNEVNRGLILYTHNGQEGMRDLVKFDVTDGINPLIDRYFYITVGSIDMVFPDVISKGVSLKEGGKVTLTTNLLSTSDLNSPDENLVFTITRAPVRGHLESTDTPGMPIVSFTQLQLAGSKIYYIHTADDEVKMDSFEFEVTDGYNPVFRTFRVSIVDVDNKKPVLTIHDLLVTEGQTKLITPFELTVEDQDTAEHLLKFKVSQLPIHGKLLFNNSQPLTSFTKQDLNENMISYKHDGTESKKDSFSFTVTDGTHSDFFVFPDTIFETQRPQTMKINIVSVDNGVPQIEVNKGAPTLKVLFAGHLGYQMTSKVLKAVDRDSNPEALIFHITTQPEHGYLISLDRGTENDSINTFTQADINDLKICYILKDENNATSDVFSFTVEDNGGNKLKNQPFHLNWSWISLEKPNYVVDEHDKVLEVTLRRRGFLGETSFVGIGTLDGSAEKEKDFLGKSQKQVQFNPGQTTASWKVRVLSDGKYEQSETFQIVLTEPVMGVLEAPAVATVEIVDPGDESMVFFPQAGFAVEEDIGELLVPVRRTGDVSQELMVVCHTQQDSATGTVPTSVLSYSDYISRPEEQVSILRFDKDETEKHCRVVIIDDSLYESEESFNVTLSMPIGGRLGPEYPTVNIRILPDQDDAPVFYFGSQEYHVDESDGHVEVRVWRTGTDLSKTATVTVRSRKTDPVSAEAGVDYVGISRNLDFAPGVSMQTVRVTVLDDLGQPVLEGTERFELVLRMPSNGILGEPAKVTVFINDSISDLPKVQFKQAVVTVEESEGHVTVMVHRSGDIKHKSSVRCYTRQGSAQVVYDFEERPNTDASIVTFLPGEVDKPCVLKLVDDSIHEEEEELRLVLGNAKSDSPYGASIGNQNETLIKIKDNADKSIIKFAETKFSVSEPKESGQVTLVKIPIRRTGDSSKVSLVRVHTKDGSAISGEDYHPISLDVEFKAEEIEHVVEVEVLFDGIREMREAFTVHLKPDENMIAEIQMTKAIVYIEETNSMADVTFPSLPHIVSLLHYDNIRTSKQSAHPPAGYPVVCVTACNPKYPDYDKTGSICISERINNTLTRYRWLISAPSGPDGVTSPMREADFDTFFTSSKMITLDSIYFQAGSRVQCAARAVNSNGDEGLELSSPIVSISMEEGMCQPRKLGTVGAEPFSAKLRYTGAEDLEYANLIKLTVTMPHIDGMLPVVSTRALSNFDLTLSPDGTRVGNHRCSNLLDFNEVSTRHGFLTKATRNIQSVGETLPYQYSPVLRGQGTLRFYRNLNLEACLWEFVSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLHVSYVFHSPVGAGGWQHFDLHSELRLTFVYDTAILWKDGVGSQTQADLQGNLGALYPTSMRINEQGRLVVNFRTEARFRGLFVVGHPASTVSTVMSADHPGLTFNLTLVRTEPTYSQPVQQWMFISDFAVRDYSGTYTVSLIPCTAALNVEFTVPPVCNPREAITFDLDIRFQQVSDPVAVEFSLNTQMFLLSKKVLWLSDGSMGFGQESDAAFSEGDVIYGRVMVDPVQNLGDSFYCNIEKVYLCTGADGYVPKYNPSKSEFGCLADSPSLLYQFKIIDKAQPETQARSFGNVDFNALLAVDDPSALSLVKQPGSDGFRLHSTPLFQVAVGREWFIHTIYTVRSKDNAKRGIGKRSVEQHLITSGDLKESRRRSRRSNRDVPTLAEQIGPENNRGTNIMHIALDRSRRTASEEELFAEGLSPRGLSGRQAEEEAVTVAGALVGLLLTVLVFVALALVVRSRRTPAKKAPRVSPSLQPVIVRSSLDNGDSSEV

Target ORF information:

RefSeq Version	NM_001137657.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio FRAS1 related extracellular matrix 2a (frem2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001137657.1

ORF Insert Sequence:

ATGGAACTTC ACAGATTTCT TCTCCTCCTG CTATCACTGA CAGGCAGTTC CATGAGCGCA 
CTTGAAACGC ACGGACTGTC GTACGCGTCC AAGCCTGAAC TCTCCGAGGA CGCTATACTG 
GTGGCAAACA ACGGCATACA TGTGCCTTTC GGGAGATCTG TTTACATAGA TCCGATCAAC 
GACTTGGTTA TTGAACTTCA GCCGGGAGAC AGGTGCACTA TAACAGTTCT GGATAATGAT 
CCGCTGTCTC AGAGACCGGG ACACGTGTCT CCTAAAAAGT TCCCGTGCGA ATTCGGTCCT 
AACGATGTGA GATACTCTCA TTTCGGTTCT AGGAGCCCTG CAGGGGACAG AGTGAAGCTG 
CAACTCAGGT ATGACACACA GACTGAAACT ATCATCATCC CCTTTATGCT GGAAGTGGAG 
GTTGTTTTCA CACAATTGGA GGTTGTTACT AAGAACATGC CTCTGGTTGT TGATAAACTA 
CATGGAACTA GTAATCCTAT TAATAAAAAG ATTTTAGATT TCACTTATGA AAAAGACTCT 
GAACAATGTG AGGTAACGTC ACTGGCTGCC AGCAGTGTTC TTCCTAAATA CGGTAAAGTT 
GTTGATGATA AAAAGCTGGA TAAAATGATG AGCTGTGAGT TGTTCCTCAA ATCAAATATC 
CGTTATGAGC ACACGTCTAG CCACAAGTCC CCTAATAAGG ATTACATCCC CATGGTTGTG 
GAGCTACGAA ACAAAGAAAA TAATTTGCTG AAACAGGAGT TTTTCCATGT GATGATCCGG 
ATCAAAGAGG GTGAAGAAAA CACTCCCCCT AGACCCAGTT TTGTGGCTAT GATGATGATG 
GAAGTTGATC AGTTTGTAAT GACGGCTTTA ACTCCTGATA TGTTGGCTGC TGAGGACACT 
GAATCCAATT CTGATGATCT CATTTTCAAC ATCACTTCTC CTCTGTCCAT TGAGGAGGGT 
TATATCGTGA GTACAGATGA CAGGAATTTA CCGATTACAT CCTTTTATCA AAAGGATCTG 
AAAGATCTGA AAATAGCATA CAAACCCCCC TCTTTGGACT CAGACACTGA GCGTATCTTT 
CAAATAGAGT TTGAGGTCAT CGACCCAGAT GGTGGCATTT CAGATCCTTT TGCTTTCATG 
ATCGTTGTGA AGCCCATGAA TACATTAGCG CCGATAGTCA CTAAAAATTC TGGCCAGCTT 
TTATATGAAG GCCAGTCTAG GTCTTTGTTT ACTCAAAACA ATTTAGAAAT TAGCGACGAA 
GACAACCTGG ACAGTGTGAG AATAACAGTG GTTGATGGGC TGCGTCATGG TGTCCTAACT 
GTTTTGGGCT CCAACAGAAA GTATTTTTCT CCGGCTGACT TGGAGGTTGG CATTGTGGTT 
TACCAGCATG ATGGCAGTGA CACATACAGT GATAATATAA TATTCAGAAT GACAGACGGG 
AAGAATGAAG TGGAGTTTTT GTTCCCCATC ACAATAGTAC CCACAGACGA TGAACCACCC 
ATCATAAATG CAAACACTGG ACTGATATTA TTCAAAAATC AGATGGTGCC CATCTCGCAG 
CTCATGCTCA GTGCTGCAGA TATTGACTCT GAAGATTCCA TTATTAAATT TACAATCGAG 
CCTCCCTTTT CAGCTATTGG CGAGGTCGTG TTGAGGCAAT CTGAAGCACC AGAGGATCCG 
TCATCCTGGA AATTTAATGC AGACGACGGT GTGTATGAGA AAGTAGTGAC AGAGTGGCTC 
CAGAAAGATA TTACAAATGA AAAGCTCTTC TATAGGCATA CGGGTCCACA TATCACAGAG 
ACTGTTACAG ATCAGTTTGT GTTTAAAGTG AAAGATGACG CTGATCCTCC CAACCAGTCC 
AGTGAGAATA ATTTTATTAT TAAAATTTTG CCTATTGATG ATATTCCACC CGTGCTGTTC 
CCAGGCACTA CTTTACAGAT GACAGTACAT GAGTACCAGC TGACTCATTT AAGCAAGAAT 
GTTCTTCGTT ACACCGACTT GGACTCAGAG GACCGGGACC TGAAATACAC AATAACTCAA 
GCACCAACTG ATATGGATGA AAACAATCCT GTCAAATTTG GCTCTATTGT TTTAACTGAC 
AGCCCCGATA CTGATGTAAC CGAATTTACC CAAGCGCAAA TTAACCATCA TAAGATTTCC 
TACAACCCGC CAGACCTTGA ACTCGGCATC ATACCTCATG TGGTGCAGTT CCATTTCACA 
GTAGAGGACA CTGCTGGAAA TGGTGCTGAT GGAGTTTTTA CAATCTTTCT GCAGCCTGTA 
AACAACAAAC CCCCACAGAT AACAAACTCT GGATTCACAG TCCTTGAGAG AGGGGTGCAT 
GTTATTACGA GCGCCGAACT TGACGCCAAT GACCTTGATA CTGACATTAA GCTAATATCA 
TTCACGCTAA GTCAAACTCC ACTGCACGGT CATCTTCAAC ATACGTTTAC TGACTTAAAA 
AAAGGGAGTC ATTTCAACTT GATGGATATT TCTGATGGTC AGGTGTCATA TGTCCACAAT 
GGGGATGAAA CGACAAGTGA CTCTTTTAAA CTAGATGTTA GTGACGGTGT TCATGTTGTA 
ACGATCACTG TGAAGGTAAC TGTAAAGCTT ATTGATGATG AAACACCAAC ACTTAGCCTC 
ACAAAGGGCA CCTTTGGCTC TAGATTGGAT GTGCTGGAAA ATGGGGCTAC AGAAATTACT 
CCCAATGTCA TTCAGGGGAA AGATGAGGAC ACTGATGACC TGCAGTTGAC ATTCATTGTG 
GAAGAAGCAC CTAAGTTTGG AGAAATTCTG GTGAATGGAG TTTCCTCGAG CAGATTCACC 
CAAGCTGACA TCATCAGTGG TCTTGTTGTG TATGCGCACA CCAGTGGTGA GATTGGTCTT 
ACTTCAATTG AAGATTTTTT CAGTTTGACC CTCTCTGACA TGTCTGATGA GTGGAATGTA 
GGGGGTAACC GAGTACACGG AGTCCGTGTT CATGTCACCA TTCTCCCTGT AGATAACCAG 
ACTCCATTGG TCACAGTTGG CCCTACTTTT AATGTCTTTG AAGCAGATAA AAACATTATC 
GGGAGCCATC AGATTAAAGT CGAGGATGCC GACACACCAA ACGAGGACAT CCTGTGCACC 
ATTATCGTTC AGCCCACATC AGGATATGTG GAGAACATTT CTCCAGCTCC AGGCTCTGAA 
AAGTCAAGAG CAGGAACGGC AGTGAGTGCT TTCACCATCA GTGATGTGAA TGAAGGTCAT 
ATTTATTATG TCCAAAGCAT ACACAAAGGA GTTGAGCCAG TGGAGGACAG GTTCACGTTC 
AGATGTTCTG ATGGTGTGAA TTTCTCAGAG AGACAGTTCT TTCCCATTGT CATTATCCCC 
ACAAATGATG AAAAACCAGA AATCTTCATG CGAGAGGTTG TTGTAATGGA GGGTATGAAT 
GTTATTATTG ACACGCCACT CTTGAATGGA GTCGATGCTG ATGTTCCTGC TCAAGAGTTG 
ATGTTTATTA TCACCAAACT TCCCAATCAT GGGTTCATTC TCAATCAGCT CGCCAGTGGC 
TCTGTCCCAG TCACAAACTT CACTCTGGAA CAAATCAAAG AAGCCTCTAG CATAATTTAC 
GAACATGATG ATTCTGAAAC CACCGAAGAC AAGTTTGATG TTCTTCTTAC AGATGGGAAA 
CATAAAGCTG AAGGCACAGT CATGATCATG ATTATTCCAG TGGATGATGA AACACCAAGG 
CTTGCCATAA ATGACGGGCT TGAAATTGAG ATTGGTGAAA CAAAAGAGAT AAATGCTAAA 
GTGCTGAAAG CTACTGATTT GGACTCAGAC GACGAGTCCC TTACTTTCAT TATTCGCTAC 
GGCCCAGCTC AAGGCTTACT GTATAGAAAA TCAGCAGGAT CTGTGGAAAA GAGATCTGCT 
GGATCTACAG AGAACATAAC AGTTGGCATG AACTTTACAC AAAATGAGGT GAACAGAGGG 
CTCATTTTGT ACACACACAA TGGACAGGAA GGCATGCGTG ATCTTGTAAA GTTTGATGTA 
ACTGACGGCA TCAATCCCCT AATTGACAGA TACTTCTATA TCACTGTGGG CAGCATTGAC 
ATGGTGTTTC CTGATGTCAT CAGTAAGGGG GTGTCTCTTA AAGAAGGTGG CAAAGTGACA 
CTCACTACGA ACCTCCTCAG CACTAGTGAT CTCAACAGCC CCGATGAGAA TTTAGTCTTT 
ACTATCACAA GAGCCCCAGT GAGAGGACAT TTGGAAAGCA CGGACACACC AGGCATGCCT 
ATAGTATCCT TTACCCAATT ACAACTGGCC GGGAGCAAGA TATACTACAT CCATACTGCT 
GATGATGAAG TCAAGATGGA CAGCTTTGAG TTTGAGGTGA CCGATGGTTA CAACCCTGTG 
TTTCGCACAT TTCGCGTTTC AATCGTTGAT GTTGACAATA AAAAGCCAGT ATTGACCATT 
CATGACCTTC TAGTAACGGA GGGACAAACC AAACTCATTA CACCCTTTGA GTTGACCGTT 
GAGGACCAAG ACACTGCCGA GCACCTTCTG AAGTTTAAAG TCTCCCAATT GCCCATTCAT 
GGGAAACTAC TTTTCAACAA CAGCCAGCCA TTGACTTCTT TCACAAAGCA AGACCTAAAT 
GAAAACATGA TCAGTTACAA GCATGATGGA ACGGAGTCGA AAAAAGATAG CTTTTCATTT 
ACAGTCACCG ATGGTACCCA TTCAGACTTT TTTGTATTCC CTGATACAAT TTTTGAGACC 
CAAAGGCCTC AGACCATGAA GATCAACATT GTGTCTGTGG ACAATGGCGT GCCTCAGATT 
GAGGTTAACA AAGGTGCACC AACTTTAAAA GTATTGTTCG CTGGACACTT GGGTTATCAG 
ATGACAAGCA AAGTCCTGAA GGCAGTGGAC AGAGACAGCA ACCCAGAGGC TTTAATTTTT 
CACATTACCA CACAGCCAGA GCATGGTTAT CTCATTAGCC TGGACAGAGG GACGGAGAAT 
GACTCTATCA ACACCTTCAC ACAAGCTGAC ATAAATGATC TTAAGATCTG CTATATCCTG 
AAGGACGAGA ACAATGCCAC CAGTGACGTC TTTAGTTTTA CCGTGGAGGA TAATGGAGGG 
AACAAACTGA AGAACCAACC GTTTCATCTG AACTGGTCTT GGATCTCTCT GGAGAAGCCA 
AACTATGTGG TGGATGAGCA CGACAAAGTT CTGGAGGTGA CCCTCAGACG GAGAGGTTTC 
CTGGGTGAAA CGTCTTTTGT TGGGATTGGT ACCTTGGATG GCAGTGCAGA GAAGGAGAAG 
GATTTCCTGG GAAAATCGCA GAAGCAGGTG CAGTTTAACC CTGGCCAGAC CACAGCCTCC 
TGGAAAGTCA GAGTTCTCTC GGATGGGAAA TACGAGCAGT CCGAAACGTT TCAAATTGTG 
CTGACTGAGC CGGTGATGGG CGTTTTGGAG GCGCCAGCTG TAGCCACAGT TGAGATTGTG 
GATCCAGGTG ATGAGTCCAT GGTGTTCTTT CCTCAGGCCG GCTTTGCTGT GGAAGAAGAT 
ATTGGAGAGC TGCTTGTCCC AGTGCGCAGG ACTGGAGACG TGAGCCAGGA GCTCATGGTG 
GTCTGTCACA CACAGCAAGA CTCTGCCACA GGAACTGTCC CCACATCGGT TCTGTCCTAC 
TCAGACTACA TCTCGCGTCC AGAGGAGCAG GTCAGCATCC TGCGCTTTGA TAAGGACGAG 
ACGGAAAAAC ACTGTCGTGT GGTGATCATC GATGACTCTC TGTATGAGAG CGAGGAGAGC 
TTCAACGTGA CCCTCAGCAT GCCTATAGGA GGCCGTCTGG GGCCCGAATA CCCCACTGTG 
AATATCCGCA TTCTGCCCGA CCAGGATGAT GCTCCGGTGT TCTACTTTGG CAGTCAGGAG 
TATCATGTGG ATGAGAGTGA CGGTCATGTT GAAGTGCGTG TGTGGAGAAC GGGCACAGAT 
CTCTCTAAAA CGGCAACGGT CACTGTGAGA TCCCGCAAAA CAGACCCTGT TTCTGCTGAA 
GCTGGAGTGG ACTATGTGGG AATCAGCAGA AACTTAGACT TCGCTCCGGG TGTAAGCATG 
CAGACGGTGC GGGTCACGGT GCTGGATGAC CTCGGACAGC CTGTTCTGGA AGGGACAGAA 
AGATTTGAGC TTGTTCTCCG GATGCCCTCC AATGGAATTC TTGGAGAGCC GGCCAAAGTC 
ACAGTCTTTA TCAACGACTC CATTTCAGAC CTGCCAAAGG TGCAGTTTAA ACAGGCTGTC 
GTGACTGTAG AGGAGAGTGA AGGTCATGTC ACTGTAATGG TGCACCGCAG TGGAGACATT 
AAACACAAGT CCAGTGTCCG CTGCTACACG CGGCAGGGCT CCGCACAGGT GGTATACGAC 
TTTGAGGAGA GACCAAACAC TGACGCCTCC ATCGTAACTT TCTTGCCTGG TGAAGTTGAT 
AAACCTTGTG TCTTGAAACT GGTGGATGAT TCAATTCATG AGGAGGAGGA AGAACTGAGG 
CTCGTTTTGG GAAATGCAAA GAGTGATTCG CCATACGGAG CGTCCATTGG AAACCAGAAT 
GAAACACTCA TAAAGATCAA GGACAATGCT GACAAATCCA TCATAAAGTT TGCCGAGACG 
AAGTTCAGTG TTAGCGAACC GAAAGAGTCT GGGCAGGTGA CATTAGTAAA GATCCCCATC 
CGCAGAACAG GAGACTCTTC CAAAGTGTCC CTAGTGAGGG TGCACACTAA AGACGGCTCT 
GCCATATCCG GAGAAGACTA TCATCCTATC TCCCTCGATG TGGAGTTTAA AGCAGAAGAG 
ATCGAGCATG TAGTTGAAGT GGAGGTTTTG TTTGATGGAA TAAGAGAGAT GAGAGAGGCC 
TTCACTGTCC ATTTAAAACC AGATGAGAAC ATGATTGCTG AAATACAGAT GACAAAAGCA 
ATAGTGTACA TCGAGGAGAC CAACAGCATG GCTGATGTCA CATTCCCTTC ATTGCCACAT 
ATTGTGTCAC TGCTGCACTA TGACAATATC AGAACAAGCA AACAAAGTGC TCATCCACCC 
GCTGGATATC CTGTCGTCTG TGTCACGGCC TGCAACCCCA AATATCCAGA CTACGACAAA 
ACAGGCTCCA TCTGCATTAG CGAACGCATC AACAACACGC TGACTCGTTA CCGATGGCTC 
ATTAGCGCCC CCAGCGGTCC TGATGGCGTC ACCAGCCCCA TGAGGGAGGC TGACTTCGAC 
ACCTTCTTCA CATCCTCCAA AATGATCACC CTAGACTCCA TTTACTTCCA AGCGGGCTCC 
AGAGTGCAGT GTGCAGCCCG TGCCGTGAAT TCAAATGGAG ACGAGGGTTT GGAACTGAGC 
AGCCCAATAG TGTCTATTAG CATGGAGGAA GGGATGTGCC AACCACGAAA ATTGGGCACA 
GTGGGTGCTG AGCCCTTTTC TGCCAAACTA CGTTATACGG GCGCTGAAGA CCTTGAATAT 
GCCAATCTCA TCAAGCTTAC TGTCACCATG CCCCACATAG ACGGCATGCT CCCCGTGGTC 
TCCACTCGTG CTCTGTCCAA TTTTGACCTG ACTCTGAGCC CCGATGGCAC TCGCGTGGGA 
AACCACAGGT GCTCCAATCT GCTGGACTTT AATGAGGTGT CCACACGGCA CGGCTTCCTG 
ACCAAAGCCA CCCGCAATAT CCAGAGCGTT GGAGAGACCC TACCTTATCA GTACAGCCCT 
GTCCTCCGAG GCCAGGGCAC TTTACGCTTC TACAGGAACC TGAACCTGGA GGCCTGTCTG 
TGGGAGTTTG TGAGTTATTA TGACATGTCA GAGCTGCTCA CAGACTGCGG GGGAACCATC 
GGGACAGATG GCCAGGTGCT GAACCTGGTG CAGTCATACG TGACCCTGCG TGTTCCTCTT 
CACGTCTCAT ACGTGTTTCA TTCCCCTGTG GGTGCTGGAG GCTGGCAGCA TTTCGACCTG 
CACTCCGAGC TCCGGCTCAC CTTTGTTTAT GACACTGCCA TCTTGTGGAA GGACGGAGTC 
GGCAGCCAAA CACAGGCTGA TCTACAAGGC AACCTTGGAG CTTTATATCC CACCAGCATG 
AGGATTAACG AGCAGGGGAG ACTCGTGGTC AACTTCCGCA CAGAGGCCCG CTTCAGGGGT 
TTATTTGTAG TGGGGCATCC TGCGTCCACA GTGTCTACAG TGATGAGTGC AGATCACCCA 
GGCTTGACCT TCAACCTAAC TCTGGTCAGG ACGGAGCCGA CCTACAGCCA ACCCGTCCAG 
CAGTGGATGT TTATCTCAGA CTTCGCTGTT CGTGATTATT CTGGCACATA CACAGTGAGC 
TTGATCCCCT GTACAGCTGC TCTGAATGTG GAGTTCACAG TTCCTCCAGT CTGCAACCCT 
AGAGAAGCCA TCACGTTTGA TCTCGATATC CGCTTCCAGC AGGTGAGTGA CCCTGTGGCT 
GTGGAGTTCA GTCTGAACAC ACAGATGTTC CTGCTGTCTA AGAAAGTGCT GTGGCTGTCA 
GATGGATCAA TGGGCTTTGG TCAGGAAAGT GACGCCGCTT TCTCTGAAGG TGACGTGATC 
TATGGACGTG TGATGGTGGA CCCGGTACAG AATTTGGGCG ACTCTTTCTA CTGTAACATT 
GAGAAGGTGT ATCTGTGCAC TGGAGCTGAT GGATATGTTC CTAAATACAA CCCCAGCAAG 
TCTGAGTTTG GATGCCTTGC TGACTCTCCG TCTCTGCTCT ACCAATTCAA GATTATTGAC 
AAAGCTCAGC CAGAAACTCA GGCCAGAAGT TTTGGGAATG TGGACTTCAA TGCTCTGCTG 
GCTGTAGATG ACCCATCGGC CCTCTCTCTG GTCAAACAGC CTGGATCTGA TGGCTTCAGA 
CTCCATTCTA CACCCCTCTT CCAGGTGGCA GTAGGGAGAG AGTGGTTCAT TCACACCATC 
TACACTGTGC GTTCAAAAGA CAACGCCAAA CGTGGCATCG GCAAACGAAG TGTCGAACAG 
CACTTGATAA CATCAGGAGA CCTTAAAGAG TCAAGAAGAC GCTCCAGACG CTCCAACAGA 
GACGTTCCCA CACTGGCTGA GCAGATTGGC CCCGAAAACA ATCGAGGAAC CAACATCATG 
CACATCGCTC TAGACCGCAG CCGCCGCACT GCATCTGAAG AAGAGCTGTT TGCGGAGGGA 
CTTTCTCCAC GAGGACTCAG TGGACGACAG GCAGAAGAAG AAGCGGTGAC GGTGGCAGGA 
GCTCTTGTGG GTTTGCTGCT CACCGTTCTT GTGTTCGTAG CATTAGCATT GGTGGTTCGC 
TCAAGGCGGA CGCCAGCTAA AAAGGCCCCA AGAGTATCAC CCAGCCTGCA GCCGGTCATT 
GTGCGGAGCA GTCTTGATAA TGGGGACAGT TCAGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem2a ( XM_005155380.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155437.1 Danio rerio Fras1 related extracellular matrix protein 2a (frem2a), transcript variant X1, mRNA.

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CloneID	ODa16167
Clone ID Related Accession (Same CDS sequence)	XM_005155380.3 , XM_005155380.4
Accession Version	XM_005155380.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	FRAS1-related extracellular matrix protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155380.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTTC ACAGATTTCT TCTCCTCCTG CTATCACTGA CAGGCAGTTC CATGAGCGCA 
CTTGAAACGC ACGGACTGTC GTACGCGTCC AAGCCTGAAC TCTCCGAGGA CGCTATACTG 
GTGGCAAACA ACGGCATACA TGTGCCTTTC GGGAGATCTG TTTACATAGA TCCGATCAAC 
GACTTGGTTA TTGAACTTCA GCCGGGAGAC AGGTGCACTA TAACAGTTCT GGATAATGAT 
CCGCTGTCTC AGAGACCGGG ACACGTGTCT CCTAAAAAGT TCCCGTGCGA ATTCGGTCCT 
AACGATGTGA GATACTCTCA TTTCGGTTCT AGGAGCCCTG CAGGGGACAG AGTGAAGCTG 
CAACTCAGGT ATGACACACA GACTGAAACT ATCATCATCC CCTTTATGCT GGAAGTGGAG 
GTTGTTTTCA CACAATTGGA GGTTGTTACT AAGAACATGC CTCTGGTTGT TGATAAACTA 
CATGGAACTA GTAATCCTAT TAATAAAAAG ATTTTAGATT TCACTTATGA AAAAGACTCT 
GAACAATGTG AGGTAACGTC ACTGGCTGCC AGCAGTGTTC TTCCTAAATA CGGTAAAGTT 
GTTGATGATA AAAAGCTGGA TAAAATGATG AGCTGTGAGT TGTTCCTCAA ATCAAATATC 
CGTTATGAGC ACACGTCTAG CCACAAGTCC CCTAATAAGG ATTACATCCC CATGGTTGTG 
GAGCTACGAA ACAAAGAAAA TAATTTGCTG AAACAGGAGT TTTTCCATGT GATGATCCGG 
ATCAAAGAGG GTGAAGAAAA CACTCCCCCT AGACCCAGTT TTGTGGCTAT GATGATGATG 
GAAGTTGATC AGTTTGTAAT GACGGCTTTA ACTCCTGATA TGTTGGCTGC TGAGGACACT 
GAATCCAATT CTGATGATCT CATTTTCAAC ATCACTTCTC CTCTGTCCAT TGAGGAGGGT 
TATATCGTGA GTACAGATGA CAGGAATTTA CCGATTACAT CCTTTTATCA AAAGGATCTG 
AAAGATCTGA AAATAGCATA CAAACCCCCC TCTTTGGACT CAGACACTGA GCGTATCTTT 
CAAATAGAGT TTGAGGTCAT CGACCCAGAT GGTGGCATTT CAGATCCTTT TGCTTTCATG 
ATCGTTGTGA AGCCCATGAA TACATTAGCG CCGATAGTCA CTAAAAATTC TGGCCAGCTT 
TTATATGAAG GCCAGTCTAG GTCTTTGTTT ACTCAAAACA ATTTAGAAAT TAGCGACGAA 
GACAACCTGG ACAGTGTGAG AATAACAGTG GTTGATGGGC TGCGTCATGG TGTCCTAACT 
GTTTTGGGCT CCAACAGAAA GTATTTTTCT CCGGCTGACT TGGAGGTTGG CATTGTGGTT 
TACCAGCATG ATGGCAGTGA CACATACAGT GATAATATAA TATTCAGAAT GACAGACGGG 
AAGAATGAAG TGGAGTTTTT GTTCCCCATC ACAATAGTAC CCACAGACGA TGAACCACCC 
ATCATAAATG CAAACACTGG ACTGATATTA TTCAAAAATC AGATGGTGCC CATCTCGCAG 
CTCATGCTCA GTGCTGCAGA TATTGACTCT GAAGATTCCA TTATTAAATT TACAATCGAG 
CCTCCCTTTT CAGCTATTGG CGAGGTCGTG TTGAGGCAAT CTGAAGCACC AGAGGATCCG 
TCATCCTGGA AATTTAATGC AGACGACGGT GTGTATGAGA AAGTAGTGAC AGAGTGGCTC 
CAGAAAGATA TTACAAATGA AAAGCTCTTC TATAGGCATA CGGGTCCACA TATCACAGAG 
ACTGTTACAG ATCAGTTTGT GTTTAAAGTG AAAGATGACG CTGATCCTCC CAACCAGTCC 
AGTGAGAATA ATTTTATTAT TAAAATTTTG CCTATTGATG ATATTCCACC CGTGCTGTTC 
CCAGGCACTA CTTTACAGAT GACAGTACAT GAGTACCAGC TGACTCATTT AAGCAAGAAT 
GTTCTTCGTT ACACCGACTT GGACTCAGAG GACCGGGACC TGAAATACAC AATAACTCAA 
GCACCAACTG ATATGGATGA AAACAATCCT GTCAAATTTG GCTCTATTGT TTTAACTGAC 
AGCCCCGATA CTGATGTAAC CGAATTTACC CAAGCGCAAA TTAACCATCA TAAGATTTCC 
TACAACCCGC CAGACCTTGA ACTCGGCATC ATACCTCATG TGGTGCAGTT CCATTTCACA 
GTAGAGGACA CTGCTGGAAA TGGTGCTGAT GGAGTTTTTA CAATCTTTCT GCAGCCTGTA 
AACAACAAAC CCCCACAGAT AACAAACTCT GGATTCACAG TCCTTGAGAG AGGGGTGCAT 
GTTATTACGA GCGCCGAACT TGACGCCAAT GACCTTGATA CTGACATTAA GCTAATATCA 
TTCACGCTAA GTCAAACTCC ACTGCACGGT CATCTTCAAC ATACGTTTAC TGACTTAAAA 
AAAGGGAGTC ATTTCAACTT GATGGATATT TCTGATGGTC AGGTGTCATA TGTCCACAAT 
GGGGATGAAA CGACAAGTGA CTCTTTTAAA CTAGATGTTA GTGACGGTGT TCATGTTGTA 
ACGATCACTG TGAAGGTAAC TGTAAAGCTT ATTGATGATG AAACACCAAC ACTTAGCCTC 
ACAAAGGGCA CCTTTGGCTC TAGATTGGAT GTGCTGGAAA ATGGGGCTAC AGAAATTACT 
CCCAATGTCA TTCAGGGGAA AGATGAGGAC ACTGATGACC TGCAGTTGAC ATTCATTGTG 
GAAGAAGCAC CTAAGTTTGG AGAAATTCTG GTGAATGGAG TTTCCTCGAG CAGATTCACC 
CAAGCTGACA TCATCAGTGG TCTTGTTGTG TATGCGCACA CCAGTGGTGA GATTGGTCTT 
ACTTCAATTG AAGATTTTTT CAGTTTGACC CTCTCTGACA TGTCTGATGA GTGGAATGTA 
GGGGGTAACC GAGTACACGG AGTCCGTGTT CATGTCACCA TTCTCCCTGT AGATAACCAG 
ACTCCATTGG TCACAGTTGG CCCTACTTTT AATGTCTTTG AAGCAGATAA AAACATTATC 
GGGAGCCATC AGATTAAAGT CGAGGATGCC GACACACCAA ACGAGGACAT CCTGTGCACC 
ATTATCGTTC AGCCCACATC AGGATATGTG GAGAACATTT CTCCAGCTCC AGGCTCTGAA 
AAGTCAAGAG CAGGAACGGC AGTGAGTGCT TTCACCATCA GTGATGTGAA TGAAGGTCAT 
ATTTATTATG TCCAAAGCAT ACACAAAGGA GTTGAGCCAG TGGAGGACAG GTTCACGTTC 
AGATGTTCTG ATGGTGTGAA TTTCTCAGAG AGACAGTTCT TTCCCATTGT CATTATCCCC 
ACAAATGATG AAAAACCAGA AATCTTCATG CGAGAGGTTG TTGTAATGGA GGGTATGAAT 
GTTATTATTG ACACGCCACT CTTGAATGGA GTCGATGCTG ATGTTCCTGC TCAAGAGTTG 
ATGTTTATTA TCACCAAACT TCCCAATCAT GGGTTCATTC TCAATCAGCT CGCCAGTGGC 
TCTGTCCCAG TCACAAACTT CACTCTGGAA CAAATCAAAG AAGCCTCTAG CATAATTTAC 
GAACATGATG ATTCTGAAAC CACCGAAGAC AAGTTTGATG TTCTTCTTAC AGATGGGAAA 
CATAAAGCTG AAGGCACAGT CATGATCATG ATTATTCCAG TGGATGATGA AACACCAAGG 
CTTGCCATAA ATGACGGGCT TGAAATTGAG ATTGGTGAAA CAAAAGAGAT AAATGCTAAA 
GTGCTGAAAG CTACTGATTT GGACTCAGAC GACGAGTCCC TTACTTTCAT TATTCGCTAC 
GGCCCAGCTC AAGGCTTACT GTATAGAAAA TCAGCAGGAT CTGTGGAAAA GAGATCTGCT 
GGATCTACAG AGAACATAAC AGTTGGCATG AACTTTACAC AAAATGAGGT GAACAGAGGG 
CTCATTTTGT ACACACACAA TGGACAGGAA GGCATGCGTG ATCTTGTAAA GTTTGATGTA 
ACTGACGGCA TCAATCCCCT AATTGACAGA TACTTCTATA TCACTGTGGG CAGCATTGAC 
ATGGTGTTTC CTGATGTCAT CAGTAAGGGG GTGTCTCTTA AAGAAGGTGG CAAAGTGACA 
CTCACTACGA ACCTCCTCAG CACTAGTGAT CTCAACAGCC CCGATGAGAA TTTAGTCTTT 
ACTATCACAA GAGCCCCAGT GAGAGGACAT TTGGAAAGCA CGGACACACC AGGCATGCCT 
ATAGTATCCT TTACCCAATT ACAACTGGCC GGGAGCAAGA TATACTACAT CCATACTGCT 
GATGATGAAG TCAAGATGGA CAGCTTTGAG TTTGAGGTGA CCGATGGTTA CAACCCTGTG 
TTTCGCACAT TTCGCGTTTC AATCGTTGAT GTTGACAATA AAAAGCCAGT ATTGACCATT 
CATGACCTTC TAGTAACGGA GGGACAAACC AAACTCATTA CACCCTTTGA GTTGACCGTT 
GAGGACCAAG ACACTGCCGA GCACCTTCTG AAGTTTAAAG TCTCCCAATT GCCCATTCAT 
GGGAAACTAC TTTTCAACAA CAGCCAGCCA TTGACTTCTT TCACAAAGCA AGACCTAAAT 
GAAAACATGA TCAGTTACAA GCATGATGGA ACGGAGTCGA AAAAAGATAG CTTTTCATTT 
ACAGTCACCG ATGGTACCCA TTCAGACTTT TTTGTATTCC CTGATACAAT TTTTGAGACC 
CAAAGGCCTC AGACCATGAA GATCAACATT GTGTCTGTGG ACAATGGCGT GCCTCAGATT 
GAGGTTAACA AAGGTGCACC AACTTTAAAA GTATTGTTCG CTGGACACTT GGGTTATCAG 
ATGACAAGCA AAGTCCTGAA GGCAGTGGAC AGAGACAGCA ACCCAGAGGC TTTAATTTTT 
CACATTACCA CACAGCCAGA GCATGGTTAT CTCATTAGCC TGGACAGAGG GACGGAGAAT 
GACTCTATCA ACACCTTCAC ACAAGCTGAC ATAAATGATC TTAAGATCTG CTATATCCTG 
AAGGACGAGA ACAATGCCAC CAGTGACGTC TTTAGTTTTA CCGTGGAGGA TAATGGAGGG 
AACAAACTGA AGAACCAACC GTTTCATCTG AACTGGTCTT GGATCTCTCT GGAGAAGCCA 
AACTATGTGG TGGATGAGCA CGACAAAGTT CTGGAGGTGA CCCTCAGACG GAGAGGTTTC 
CTGGGTGAAA CGTCTTTTGT TGGGATTGGT ACCTTGGATG GCAGTGCAGA GAAGGAGAAG 
GATTTCCTGG GAAAATCGCA GAAGCAGGTG CAGTTTAACC CTGGCCAGAC CACAGCCTCC 
TGGAAAGTCA GAGTTCTCTC GGATGGGAAA TACGAGCAGT CCGAAACGTT TCAAATTGTG 
CTGACTGAGC CGGTGATGGG CGTTTTGGAG GCGCCAGCTG TAGCCACAGT TGAGATTGTG 
GATCCAGGTG ATGAGTCCAT GGTGTTCTTT CCTCAGGCCG GCTTTGCTGT GGAAGAAGAT 
ATTGGAGAGC TGCTTGTCCC AGTGCGCAGG ACTGGAGACG TGAGCCAGGA GCTCATGGTG 
GTCTGTCACA CACAGCAAGA CTCTGCCACA GGAACTGTCC CCACATCGGT TCTGTCCTAC 
TCAGACTACA TCTCGCGTCC AGAGGAGCAG GTCAGCATCC TGCGCTTTGA TAAGGACGAG 
ACGGAAAAAC ACTGTCGTGT GGTGATCATC GATGACTCTC TGTATGAGAG CGAGGAGAGC 
TTCAACGTGA CCCTCAGCAT GCCTATAGGA GGCCGTCTGG GGCCCGAATA CCCCACTGTG 
AATATCCGCA TTCTGCCCGA CCAGGATGAT GCTCCGGTGT TCTACTTTGG CAGTCAGGAG 
TATCATGTGG ATGAGAGTGA CGGTCATGTT GAAGTGCGTG TGTGGAGAAC GGGCACAGAT 
CTCTCTAAAA CGGCAACGGT CACTGTGAGA TCCCGCAAAA CAGACCCTGT TTCTGCTGAA 
GCTGGAGTGG ACTATGTGGG AATCAGCAGA AACTTAGACT TCGCTCCGGG TGTAAGCATG 
CAGACGGTGC GGGTCACGGT GCTGGATGAC CTCGGACAGC CTGTTCTGGA AGGGACAGAA 
AGATTTGAGC TTGTTCTCCG GATGCCCTCC AATGGAATTC TTGGAGAGCC GGCCAAAGTC 
ACAGTCTTTA TCAACGACTC CATTTCAGAC CTGCCAAAGG TGCAGTTTAA ACAGGCTGTC 
GTGACTGTAG AGGAGAGTGA AGGTCATGTC ACTGTAATGG TGCACCGCAG TGGAGACATT 
AAACACAAGT CCAGTGTCCG CTGCTACACG CGGCAGGGCT CCGCACAGGT GGTATACGAC 
TTTGAGGAGA GACCAAACAC TGACGCCTCC ATCGTAACTT TCTTGCCTGG TGAAGTTGAT 
AAACCTTGTG TCTTGAAACT GGTGGATGAT TCAATTCATG AGGAGGAGGA AGAACTGAGG 
CTCGTTTTGG GAAATGCAAA GAGTGATTCG CCATACGGAG CGTCCATTGG AAACCAGAAT 
GAAACACTCA TAAAGATCAA GGACAATGCT GACAAATCCA TCATAAAGTT TGCCGAGACG 
AAGTTCAGTG TTAGCGAACC GAAAGAGTCT GGGCAGGTGA CATTAGTAAA GATCCCCATC 
CGCAGAACAG GAGACTCTTC CAAAGTGTCC CTAGTGAGGG TGCACACTAA AGACGGCTCT 
GCCATATCCG GAGAAGACTA TCATCCTATC TCCCTCGATG TGGAGTTTAA AGCAGAAGAG 
ATCGAGCATG TAGTTGAAGT GGAGGTTTTG TTTGATGGAA TAAGAGAGAT GAGAGAGGCC 
TTCACTGTCC ATTTAAAACC AGATGAGAAC ATGATTGCTG AAATACAGAT GACAAAAGCA 
ATAGTGTACA TCGAGGAGAC CAACAGCATG GCTGATGTCA CATTCCCTTC ATTGCCACAT 
ATTGTGTCAC TGCTGCACTA TGACAATATC AGAACAAGCA AACAAAGTGC TCATCCACCC 
GCTGGATATC CTGTCGTCTG TGTCACGGCC TGCAACCCCA AATATCCAGA CTACGACAAA 
ACAGGCTCCA TCTGCATTAG CGAACGCATC AACAACACGC TGACTCGTTA CCGATGGCTC 
ATTAGCGCCC CCAGCGGTCC TGATGGCGTC ACCAGCCCCA TGAGGGAGGC TGACTTCGAC 
ACCTTCTTCA CATCCTCCAA AATGATCACC CTAGACTCCA TTTACTTCCA AGCGGGCTCC 
AGAGTGCAGT GTGCAGCCCG TGCCGTGAAT TCAAATGGAG ACGAGGGTTT GGAACTGAGC 
AGCCCAATAG TGTCTATTAG CATGGAGGAA GGGATGTGCC AACCACGAAA ATTGGGCACA 
GTGGGTGCTG AGCCCTTTTC TGCCAAACTA CGTTATACGG GCGCTGAAGA CCTTGAATAT 
GCCAATCTCA TCAAGCTTAC TGTCACCATG CCCCACATAG ACGGCATGCT CCCCGTGGTC 
TCCACTCGTG CTCTGTCCAA TTTTGACCTG ACTCTGAGCC CCGATGGCAC TCGCGTGGGA 
AACCACAGGT GCTCCAATCT GCTGGACTTT AATGAGGTGT CCACACGGCA CGGCTTCCTG 
ACCAAAGCCA CCCGCAATAT CCAGAGCGTT GGAGAGACCC TACCTTATCA GTACAGCCCT 
GTCCTCCGAG GCCAGGGCAC TTTACGCTTC TACAGGAACC TGAACCTGGA GGCCTGTCTG 
TGGGAGTTTG TGAGTTATTA TGACATGTCA GAGCTGCTCA CAGACTGCGG GGGAACCATC 
GGGACAGATG GCCAGGTGCT GAACCTGGTG CAGTCATACG TGACCCTGCG TGTTCCTCTT 
CACGTCTCAT ACGTGTTTCA TTCCCCTGTG GGTGCTGGAG GCTGGCAGCA TTTCGACCTG 
CACTCCGAGC TCCGGCTCAC CTTTGTTTAT GACACTGCCA TCTTGTGGAA GGACGGAGTC 
GGCAGCCAAA CACAGGCTGA TCTACAAGGA GCTTTATATC CCACCAGCAT GAGGATTAAC 
GAGCAGGGGA GACTCGTGGT CAACTTCCGC ACAGAGGCCC GCTTCAGGGG TTTATTTGTA 
GTGGGGCATC CTGCAAATTT GAGGAGTCTG AAAACTACAG CGTCCACAGT GTCTACAGTG 
ATGAGTGCAG ATCACCCAGG CTTGACCTTC AACCTAACTC TGGTCAGGAC GGAGCCGACC 
TACAGCCAAC CCGTCCAGCA GTGGATGTTT ATCTCAGACT TCGCTGTTCG TGATTATTCT 
GGCACATACA CAGTGAGCTT GATCCCCTGT ACAGCTGCTC TGAATGTGGA GTTCACAGTT 
CCTCCAGTCT GCAACCCTAG AGAAGCCATC ACGTTTGATC TCGATATCCG CTTCCAGCAG 
GTGAGTGACC CTGTGGCTGT GGAGTTCAGT CTGAACACAC AGATGTTCCT GCTGTCTAAG 
AAAGTGCTGT GGCTGTCAGA TGGATCAATG GGCTTTGGTC AGGAAAGTGA CGCCGCTTTC 
TCTGAAGGTG ACGTGATCTA TGGACGTGTG ATGGTGGACC CGGTACAGAA TTTGGGCGAC 
TCTTTCTACT GTAACATTGA GAAGGTGTAT CTGTGCACTG GAGCTGATGG ATATGTTCCT 
AAATACAACC CCAGCAAGTC TGAGTTTGGA TGCCTTGCTG ACTCTCCGTC TCTGCTCTAC 
CAATTCAAGA TTATTGACAA AGCTCAGCCA GAAACTCAGG CCAGAAGTTT TGGGAATGTG 
GACTTCAATG CTCTGCTGGC TGTAGATGAC CCATCGGCCC TCTCTCTGGT CAAACAGCCT 
GGATCTGATG GCTTCAGACT CCATTCTACA CCCCTCTTCC AGGTGGCAGT AGGGAGAGAG 
TGGTTCATTC ACACCATCTA CACTGTGCGT TCAAAAGACA ACGCCAAACG TGGCATCGGC 
AAACGAAGTG TCGAACAGCA CTTGATAACA TCAGGAGACC TTAAAGAGTC AAGAAGACGC 
TCCAGACGCT CCAACAGAGA CGTTCCCACA CTGGCTGAGC AGATTGGCCC CGAAAACAAT 
CGAGGAACCA ACATCATGCA CATCGCTCTA GACCGCAGCC GCCGCACTGC ATCTGAAGAA 
GAGCTGTTTG CGGAGGGACT TTCTCCACGA GGACTCAGTG GACGACAGGC AGAAGAAGAA 
GCGGTGACGG TGGCAGGAGC TCTTGTGGGT TTGCTGCTCA CCGTTCTTGT GTTCGTAGCA 
TTAGCATTGG TGGTTCGCTC AAGGCGGACG CCAGCTAAAA AGGCCCCAAG AGTATCACCC 
AGCCTGCAGC CGGTCATTGT GCGGAGCAGT CTTGATAATG GGGACAGTTC AGAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155437.1
CDS	141..9500
Translation	MELHRFLLLLLSLTGSSMSALETHGLSYASKPELSEDAILVANNGIHVPFGRSVYIDPINDLVIELQPGDRCTITVLDNDPLSQRPGHVSPKKFPCEFGPNDVRYSHFGSRSPAGDRVKLQLRYDTQTETIIIPFMLEVEVVFTQLEVVTKNMPLVVDKLHGTSNPINKKILDFTYEKDSEQCEVTSLAASSVLPKYGKVVDDKKLDKMMSCELFLKSNIRYEHTSSHKSPNKDYIPMVVELRNKENNLLKQEFFHVMIRIKEGEENTPPRPSFVAMMMMEVDQFVMTALTPDMLAAEDTESNSDDLIFNITSPLSIEEGYIVSTDDRNLPITSFYQKDLKDLKIAYKPPSLDSDTERIFQIEFEVIDPDGGISDPFAFMIVVKPMNTLAPIVTKNSGQLLYEGQSRSLFTQNNLEISDEDNLDSVRITVVDGLRHGVLTVLGSNRKYFSPADLEVGIVVYQHDGSDTYSDNIIFRMTDGKNEVEFLFPITIVPTDDEPPIINANTGLILFKNQMVPISQLMLSAADIDSEDSIIKFTIEPPFSAIGEVVLRQSEAPEDPSSWKFNADDGVYEKVVTEWLQKDITNEKLFYRHTGPHITETVTDQFVFKVKDDADPPNQSSENNFIIKILPIDDIPPVLFPGTTLQMTVHEYQLTHLSKNVLRYTDLDSEDRDLKYTITQAPTDMDENNPVKFGSIVLTDSPDTDVTEFTQAQINHHKISYNPPDLELGIIPHVVQFHFTVEDTAGNGADGVFTIFLQPVNNKPPQITNSGFTVLERGVHVITSAELDANDLDTDIKLISFTLSQTPLHGHLQHTFTDLKKGSHFNLMDISDGQVSYVHNGDETTSDSFKLDVSDGVHVVTITVKVTVKLIDDETPTLSLTKGTFGSRLDVLENGATEITPNVIQGKDEDTDDLQLTFIVEEAPKFGEILVNGVSSSRFTQADIISGLVVYAHTSGEIGLTSIEDFFSLTLSDMSDEWNVGGNRVHGVRVHVTILPVDNQTPLVTVGPTFNVFEADKNIIGSHQIKVEDADTPNEDILCTIIVQPTSGYVENISPAPGSEKSRAGTAVSAFTISDVNEGHIYYVQSIHKGVEPVEDRFTFRCSDGVNFSERQFFPIVIIPTNDEKPEIFMREVVVMEGMNVIIDTPLLNGVDADVPAQELMFIITKLPNHGFILNQLASGSVPVTNFTLEQIKEASSIIYEHDDSETTEDKFDVLLTDGKHKAEGTVMIMIIPVDDETPRLAINDGLEIEIGETKEINAKVLKATDLDSDDESLTFIIRYGPAQGLLYRKSAGSVEKRSAGSTENITVGMNFTQNEVNRGLILYTHNGQEGMRDLVKFDVTDGINPLIDRYFYITVGSIDMVFPDVISKGVSLKEGGKVTLTTNLLSTSDLNSPDENLVFTITRAPVRGHLESTDTPGMPIVSFTQLQLAGSKIYYIHTADDEVKMDSFEFEVTDGYNPVFRTFRVSIVDVDNKKPVLTIHDLLVTEGQTKLITPFELTVEDQDTAEHLLKFKVSQLPIHGKLLFNNSQPLTSFTKQDLNENMISYKHDGTESKKDSFSFTVTDGTHSDFFVFPDTIFETQRPQTMKINIVSVDNGVPQIEVNKGAPTLKVLFAGHLGYQMTSKVLKAVDRDSNPEALIFHITTQPEHGYLISLDRGTENDSINTFTQADINDLKICYILKDENNATSDVFSFTVEDNGGNKLKNQPFHLNWSWISLEKPNYVVDEHDKVLEVTLRRRGFLGETSFVGIGTLDGSAEKEKDFLGKSQKQVQFNPGQTTASWKVRVLSDGKYEQSETFQIVLTEPVMGVLEAPAVATVEIVDPGDESMVFFPQAGFAVEEDIGELLVPVRRTGDVSQELMVVCHTQQDSATGTVPTSVLSYSDYISRPEEQVSILRFDKDETEKHCRVVIIDDSLYESEESFNVTLSMPIGGRLGPEYPTVNIRILPDQDDAPVFYFGSQEYHVDESDGHVEVRVWRTGTDLSKTATVTVRSRKTDPVSAEAGVDYVGISRNLDFAPGVSMQTVRVTVLDDLGQPVLEGTERFELVLRMPSNGILGEPAKVTVFINDSISDLPKVQFKQAVVTVEESEGHVTVMVHRSGDIKHKSSVRCYTRQGSAQVVYDFEERPNTDASIVTFLPGEVDKPCVLKLVDDSIHEEEEELRLVLGNAKSDSPYGASIGNQNETLIKIKDNADKSIIKFAETKFSVSEPKESGQVTLVKIPIRRTGDSSKVSLVRVHTKDGSAISGEDYHPISLDVEFKAEEIEHVVEVEVLFDGIREMREAFTVHLKPDENMIAEIQMTKAIVYIEETNSMADVTFPSLPHIVSLLHYDNIRTSKQSAHPPAGYPVVCVTACNPKYPDYDKTGSICISERINNTLTRYRWLISAPSGPDGVTSPMREADFDTFFTSSKMITLDSIYFQAGSRVQCAARAVNSNGDEGLELSSPIVSISMEEGMCQPRKLGTVGAEPFSAKLRYTGAEDLEYANLIKLTVTMPHIDGMLPVVSTRALSNFDLTLSPDGTRVGNHRCSNLLDFNEVSTRHGFLTKATRNIQSVGETLPYQYSPVLRGQGTLRFYRNLNLEACLWEFVSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLHVSYVFHSPVGAGGWQHFDLHSELRLTFVYDTAILWKDGVGSQTQADLQGALYPTSMRINEQGRLVVNFRTEARFRGLFVVGHPANLRSLKTTASTVSTVMSADHPGLTFNLTLVRTEPTYSQPVQQWMFISDFAVRDYSGTYTVSLIPCTAALNVEFTVPPVCNPREAITFDLDIRFQQVSDPVAVEFSLNTQMFLLSKKVLWLSDGSMGFGQESDAAFSEGDVIYGRVMVDPVQNLGDSFYCNIEKVYLCTGADGYVPKYNPSKSEFGCLADSPSLLYQFKIIDKAQPETQARSFGNVDFNALLAVDDPSALSLVKQPGSDGFRLHSTPLFQVAVGREWFIHTIYTVRSKDNAKRGIGKRSVEQHLITSGDLKESRRRSRRSNRDVPTLAEQIGPENNRGTNIMHIALDRSRRTASEEELFAEGLSPRGLSGRQAEEEAVTVAGALVGLLLTVLVFVALALVVRSRRTPAKKAPRVSPSLQPVIVRSSLDNGDSSEV

Target ORF information:

RefSeq Version	XM_005155380.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Fras1 related extracellular matrix protein 2a (frem2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155380.3

ORF Insert Sequence:

ATGGAACTTC ACAGATTTCT TCTCCTCCTG CTATCACTGA CAGGCAGTTC CATGAGCGCA 
CTTGAAACGC ACGGACTGTC GTACGCGTCC AAGCCTGAAC TCTCCGAGGA CGCTATACTG 
GTGGCAAACA ACGGCATACA TGTGCCTTTC GGGAGATCTG TTTACATAGA TCCGATCAAC 
GACTTGGTTA TTGAACTTCA GCCGGGAGAC AGGTGCACTA TAACAGTTCT GGATAATGAT 
CCGCTGTCTC AGAGACCGGG ACACGTGTCT CCTAAAAAGT TCCCGTGCGA ATTCGGTCCT 
AACGATGTGA GATACTCTCA TTTCGGTTCT AGGAGCCCTG CAGGGGACAG AGTGAAGCTG 
CAACTCAGGT ATGACACACA GACTGAAACT ATCATCATCC CCTTTATGCT GGAAGTGGAG 
GTTGTTTTCA CACAATTGGA GGTTGTTACT AAGAACATGC CTCTGGTTGT TGATAAACTA 
CATGGAACTA GTAATCCTAT TAATAAAAAG ATTTTAGATT TCACTTATGA AAAAGACTCT 
GAACAATGTG AGGTAACGTC ACTGGCTGCC AGCAGTGTTC TTCCTAAATA CGGTAAAGTT 
GTTGATGATA AAAAGCTGGA TAAAATGATG AGCTGTGAGT TGTTCCTCAA ATCAAATATC 
CGTTATGAGC ACACGTCTAG CCACAAGTCC CCTAATAAGG ATTACATCCC CATGGTTGTG 
GAGCTACGAA ACAAAGAAAA TAATTTGCTG AAACAGGAGT TTTTCCATGT GATGATCCGG 
ATCAAAGAGG GTGAAGAAAA CACTCCCCCT AGACCCAGTT TTGTGGCTAT GATGATGATG 
GAAGTTGATC AGTTTGTAAT GACGGCTTTA ACTCCTGATA TGTTGGCTGC TGAGGACACT 
GAATCCAATT CTGATGATCT CATTTTCAAC ATCACTTCTC CTCTGTCCAT TGAGGAGGGT 
TATATCGTGA GTACAGATGA CAGGAATTTA CCGATTACAT CCTTTTATCA AAAGGATCTG 
AAAGATCTGA AAATAGCATA CAAACCCCCC TCTTTGGACT CAGACACTGA GCGTATCTTT 
CAAATAGAGT TTGAGGTCAT CGACCCAGAT GGTGGCATTT CAGATCCTTT TGCTTTCATG 
ATCGTTGTGA AGCCCATGAA TACATTAGCG CCGATAGTCA CTAAAAATTC TGGCCAGCTT 
TTATATGAAG GCCAGTCTAG GTCTTTGTTT ACTCAAAACA ATTTAGAAAT TAGCGACGAA 
GACAACCTGG ACAGTGTGAG AATAACAGTG GTTGATGGGC TGCGTCATGG TGTCCTAACT 
GTTTTGGGCT CCAACAGAAA GTATTTTTCT CCGGCTGACT TGGAGGTTGG CATTGTGGTT 
TACCAGCATG ATGGCAGTGA CACATACAGT GATAATATAA TATTCAGAAT GACAGACGGG 
AAGAATGAAG TGGAGTTTTT GTTCCCCATC ACAATAGTAC CCACAGACGA TGAACCACCC 
ATCATAAATG CAAACACTGG ACTGATATTA TTCAAAAATC AGATGGTGCC CATCTCGCAG 
CTCATGCTCA GTGCTGCAGA TATTGACTCT GAAGATTCCA TTATTAAATT TACAATCGAG 
CCTCCCTTTT CAGCTATTGG CGAGGTCGTG TTGAGGCAAT CTGAAGCACC AGAGGATCCG 
TCATCCTGGA AATTTAATGC AGACGACGGT GTGTATGAGA AAGTAGTGAC AGAGTGGCTC 
CAGAAAGATA TTACAAATGA AAAGCTCTTC TATAGGCATA CGGGTCCACA TATCACAGAG 
ACTGTTACAG ATCAGTTTGT GTTTAAAGTG AAAGATGACG CTGATCCTCC CAACCAGTCC 
AGTGAGAATA ATTTTATTAT TAAAATTTTG CCTATTGATG ATATTCCACC CGTGCTGTTC 
CCAGGCACTA CTTTACAGAT GACAGTACAT GAGTACCAGC TGACTCATTT AAGCAAGAAT 
GTTCTTCGTT ACACCGACTT GGACTCAGAG GACCGGGACC TGAAATACAC AATAACTCAA 
GCACCAACTG ATATGGATGA AAACAATCCT GTCAAATTTG GCTCTATTGT TTTAACTGAC 
AGCCCCGATA CTGATGTAAC CGAATTTACC CAAGCGCAAA TTAACCATCA TAAGATTTCC 
TACAACCCGC CAGACCTTGA ACTCGGCATC ATACCTCATG TGGTGCAGTT CCATTTCACA 
GTAGAGGACA CTGCTGGAAA TGGTGCTGAT GGAGTTTTTA CAATCTTTCT GCAGCCTGTA 
AACAACAAAC CCCCACAGAT AACAAACTCT GGATTCACAG TCCTTGAGAG AGGGGTGCAT 
GTTATTACGA GCGCCGAACT TGACGCCAAT GACCTTGATA CTGACATTAA GCTAATATCA 
TTCACGCTAA GTCAAACTCC ACTGCACGGT CATCTTCAAC ATACGTTTAC TGACTTAAAA 
AAAGGGAGTC ATTTCAACTT GATGGATATT TCTGATGGTC AGGTGTCATA TGTCCACAAT 
GGGGATGAAA CGACAAGTGA CTCTTTTAAA CTAGATGTTA GTGACGGTGT TCATGTTGTA 
ACGATCACTG TGAAGGTAAC TGTAAAGCTT ATTGATGATG AAACACCAAC ACTTAGCCTC 
ACAAAGGGCA CCTTTGGCTC TAGATTGGAT GTGCTGGAAA ATGGGGCTAC AGAAATTACT 
CCCAATGTCA TTCAGGGGAA AGATGAGGAC ACTGATGACC TGCAGTTGAC ATTCATTGTG 
GAAGAAGCAC CTAAGTTTGG AGAAATTCTG GTGAATGGAG TTTCCTCGAG CAGATTCACC 
CAAGCTGACA TCATCAGTGG TCTTGTTGTG TATGCGCACA CCAGTGGTGA GATTGGTCTT 
ACTTCAATTG AAGATTTTTT CAGTTTGACC CTCTCTGACA TGTCTGATGA GTGGAATGTA 
GGGGGTAACC GAGTACACGG AGTCCGTGTT CATGTCACCA TTCTCCCTGT AGATAACCAG 
ACTCCATTGG TCACAGTTGG CCCTACTTTT AATGTCTTTG AAGCAGATAA AAACATTATC 
GGGAGCCATC AGATTAAAGT CGAGGATGCC GACACACCAA ACGAGGACAT CCTGTGCACC 
ATTATCGTTC AGCCCACATC AGGATATGTG GAGAACATTT CTCCAGCTCC AGGCTCTGAA 
AAGTCAAGAG CAGGAACGGC AGTGAGTGCT TTCACCATCA GTGATGTGAA TGAAGGTCAT 
ATTTATTATG TCCAAAGCAT ACACAAAGGA GTTGAGCCAG TGGAGGACAG GTTCACGTTC 
AGATGTTCTG ATGGTGTGAA TTTCTCAGAG AGACAGTTCT TTCCCATTGT CATTATCCCC 
ACAAATGATG AAAAACCAGA AATCTTCATG CGAGAGGTTG TTGTAATGGA GGGTATGAAT 
GTTATTATTG ACACGCCACT CTTGAATGGA GTCGATGCTG ATGTTCCTGC TCAAGAGTTG 
ATGTTTATTA TCACCAAACT TCCCAATCAT GGGTTCATTC TCAATCAGCT CGCCAGTGGC 
TCTGTCCCAG TCACAAACTT CACTCTGGAA CAAATCAAAG AAGCCTCTAG CATAATTTAC 
GAACATGATG ATTCTGAAAC CACCGAAGAC AAGTTTGATG TTCTTCTTAC AGATGGGAAA 
CATAAAGCTG AAGGCACAGT CATGATCATG ATTATTCCAG TGGATGATGA AACACCAAGG 
CTTGCCATAA ATGACGGGCT TGAAATTGAG ATTGGTGAAA CAAAAGAGAT AAATGCTAAA 
GTGCTGAAAG CTACTGATTT GGACTCAGAC GACGAGTCCC TTACTTTCAT TATTCGCTAC 
GGCCCAGCTC AAGGCTTACT GTATAGAAAA TCAGCAGGAT CTGTGGAAAA GAGATCTGCT 
GGATCTACAG AGAACATAAC AGTTGGCATG AACTTTACAC AAAATGAGGT GAACAGAGGG 
CTCATTTTGT ACACACACAA TGGACAGGAA GGCATGCGTG ATCTTGTAAA GTTTGATGTA 
ACTGACGGCA TCAATCCCCT AATTGACAGA TACTTCTATA TCACTGTGGG CAGCATTGAC 
ATGGTGTTTC CTGATGTCAT CAGTAAGGGG GTGTCTCTTA AAGAAGGTGG CAAAGTGACA 
CTCACTACGA ACCTCCTCAG CACTAGTGAT CTCAACAGCC CCGATGAGAA TTTAGTCTTT 
ACTATCACAA GAGCCCCAGT GAGAGGACAT TTGGAAAGCA CGGACACACC AGGCATGCCT 
ATAGTATCCT TTACCCAATT ACAACTGGCC GGGAGCAAGA TATACTACAT CCATACTGCT 
GATGATGAAG TCAAGATGGA CAGCTTTGAG TTTGAGGTGA CCGATGGTTA CAACCCTGTG 
TTTCGCACAT TTCGCGTTTC AATCGTTGAT GTTGACAATA AAAAGCCAGT ATTGACCATT 
CATGACCTTC TAGTAACGGA GGGACAAACC AAACTCATTA CACCCTTTGA GTTGACCGTT 
GAGGACCAAG ACACTGCCGA GCACCTTCTG AAGTTTAAAG TCTCCCAATT GCCCATTCAT 
GGGAAACTAC TTTTCAACAA CAGCCAGCCA TTGACTTCTT TCACAAAGCA AGACCTAAAT 
GAAAACATGA TCAGTTACAA GCATGATGGA ACGGAGTCGA AAAAAGATAG CTTTTCATTT 
ACAGTCACCG ATGGTACCCA TTCAGACTTT TTTGTATTCC CTGATACAAT TTTTGAGACC 
CAAAGGCCTC AGACCATGAA GATCAACATT GTGTCTGTGG ACAATGGCGT GCCTCAGATT 
GAGGTTAACA AAGGTGCACC AACTTTAAAA GTATTGTTCG CTGGACACTT GGGTTATCAG 
ATGACAAGCA AAGTCCTGAA GGCAGTGGAC AGAGACAGCA ACCCAGAGGC TTTAATTTTT 
CACATTACCA CACAGCCAGA GCATGGTTAT CTCATTAGCC TGGACAGAGG GACGGAGAAT 
GACTCTATCA ACACCTTCAC ACAAGCTGAC ATAAATGATC TTAAGATCTG CTATATCCTG 
AAGGACGAGA ACAATGCCAC CAGTGACGTC TTTAGTTTTA CCGTGGAGGA TAATGGAGGG 
AACAAACTGA AGAACCAACC GTTTCATCTG AACTGGTCTT GGATCTCTCT GGAGAAGCCA 
AACTATGTGG TGGATGAGCA CGACAAAGTT CTGGAGGTGA CCCTCAGACG GAGAGGTTTC 
CTGGGTGAAA CGTCTTTTGT TGGGATTGGT ACCTTGGATG GCAGTGCAGA GAAGGAGAAG 
GATTTCCTGG GAAAATCGCA GAAGCAGGTG CAGTTTAACC CTGGCCAGAC CACAGCCTCC 
TGGAAAGTCA GAGTTCTCTC GGATGGGAAA TACGAGCAGT CCGAAACGTT TCAAATTGTG 
CTGACTGAGC CGGTGATGGG CGTTTTGGAG GCGCCAGCTG TAGCCACAGT TGAGATTGTG 
GATCCAGGTG ATGAGTCCAT GGTGTTCTTT CCTCAGGCCG GCTTTGCTGT GGAAGAAGAT 
ATTGGAGAGC TGCTTGTCCC AGTGCGCAGG ACTGGAGACG TGAGCCAGGA GCTCATGGTG 
GTCTGTCACA CACAGCAAGA CTCTGCCACA GGAACTGTCC CCACATCGGT TCTGTCCTAC 
TCAGACTACA TCTCGCGTCC AGAGGAGCAG GTCAGCATCC TGCGCTTTGA TAAGGACGAG 
ACGGAAAAAC ACTGTCGTGT GGTGATCATC GATGACTCTC TGTATGAGAG CGAGGAGAGC 
TTCAACGTGA CCCTCAGCAT GCCTATAGGA GGCCGTCTGG GGCCCGAATA CCCCACTGTG 
AATATCCGCA TTCTGCCCGA CCAGGATGAT GCTCCGGTGT TCTACTTTGG CAGTCAGGAG 
TATCATGTGG ATGAGAGTGA CGGTCATGTT GAAGTGCGTG TGTGGAGAAC GGGCACAGAT 
CTCTCTAAAA CGGCAACGGT CACTGTGAGA TCCCGCAAAA CAGACCCTGT TTCTGCTGAA 
GCTGGAGTGG ACTATGTGGG AATCAGCAGA AACTTAGACT TCGCTCCGGG TGTAAGCATG 
CAGACGGTGC GGGTCACGGT GCTGGATGAC CTCGGACAGC CTGTTCTGGA AGGGACAGAA 
AGATTTGAGC TTGTTCTCCG GATGCCCTCC AATGGAATTC TTGGAGAGCC GGCCAAAGTC 
ACAGTCTTTA TCAACGACTC CATTTCAGAC CTGCCAAAGG TGCAGTTTAA ACAGGCTGTC 
GTGACTGTAG AGGAGAGTGA AGGTCATGTC ACTGTAATGG TGCACCGCAG TGGAGACATT 
AAACACAAGT CCAGTGTCCG CTGCTACACG CGGCAGGGCT CCGCACAGGT GGTATACGAC 
TTTGAGGAGA GACCAAACAC TGACGCCTCC ATCGTAACTT TCTTGCCTGG TGAAGTTGAT 
AAACCTTGTG TCTTGAAACT GGTGGATGAT TCAATTCATG AGGAGGAGGA AGAACTGAGG 
CTCGTTTTGG GAAATGCAAA GAGTGATTCG CCATACGGAG CGTCCATTGG AAACCAGAAT 
GAAACACTCA TAAAGATCAA GGACAATGCT GACAAATCCA TCATAAAGTT TGCCGAGACG 
AAGTTCAGTG TTAGCGAACC GAAAGAGTCT GGGCAGGTGA CATTAGTAAA GATCCCCATC 
CGCAGAACAG GAGACTCTTC CAAAGTGTCC CTAGTGAGGG TGCACACTAA AGACGGCTCT 
GCCATATCCG GAGAAGACTA TCATCCTATC TCCCTCGATG TGGAGTTTAA AGCAGAAGAG 
ATCGAGCATG TAGTTGAAGT GGAGGTTTTG TTTGATGGAA TAAGAGAGAT GAGAGAGGCC 
TTCACTGTCC ATTTAAAACC AGATGAGAAC ATGATTGCTG AAATACAGAT GACAAAAGCA 
ATAGTGTACA TCGAGGAGAC CAACAGCATG GCTGATGTCA CATTCCCTTC ATTGCCACAT 
ATTGTGTCAC TGCTGCACTA TGACAATATC AGAACAAGCA AACAAAGTGC TCATCCACCC 
GCTGGATATC CTGTCGTCTG TGTCACGGCC TGCAACCCCA AATATCCAGA CTACGACAAA 
ACAGGCTCCA TCTGCATTAG CGAACGCATC AACAACACGC TGACTCGTTA CCGATGGCTC 
ATTAGCGCCC CCAGCGGTCC TGATGGCGTC ACCAGCCCCA TGAGGGAGGC TGACTTCGAC 
ACCTTCTTCA CATCCTCCAA AATGATCACC CTAGACTCCA TTTACTTCCA AGCGGGCTCC 
AGAGTGCAGT GTGCAGCCCG TGCCGTGAAT TCAAATGGAG ACGAGGGTTT GGAACTGAGC 
AGCCCAATAG TGTCTATTAG CATGGAGGAA GGGATGTGCC AACCACGAAA ATTGGGCACA 
GTGGGTGCTG AGCCCTTTTC TGCCAAACTA CGTTATACGG GCGCTGAAGA CCTTGAATAT 
GCCAATCTCA TCAAGCTTAC TGTCACCATG CCCCACATAG ACGGCATGCT CCCCGTGGTC 
TCCACTCGTG CTCTGTCCAA TTTTGACCTG ACTCTGAGCC CCGATGGCAC TCGCGTGGGA 
AACCACAGGT GCTCCAATCT GCTGGACTTT AATGAGGTGT CCACACGGCA CGGCTTCCTG 
ACCAAAGCCA CCCGCAATAT CCAGAGCGTT GGAGAGACCC TACCTTATCA GTACAGCCCT 
GTCCTCCGAG GCCAGGGCAC TTTACGCTTC TACAGGAACC TGAACCTGGA GGCCTGTCTG 
TGGGAGTTTG TGAGTTATTA TGACATGTCA GAGCTGCTCA CAGACTGCGG GGGAACCATC 
GGGACAGATG GCCAGGTGCT GAACCTGGTG CAGTCATACG TGACCCTGCG TGTTCCTCTT 
CACGTCTCAT ACGTGTTTCA TTCCCCTGTG GGTGCTGGAG GCTGGCAGCA TTTCGACCTG 
CACTCCGAGC TCCGGCTCAC CTTTGTTTAT GACACTGCCA TCTTGTGGAA GGACGGAGTC 
GGCAGCCAAA CACAGGCTGA TCTACAAGGA GCTTTATATC CCACCAGCAT GAGGATTAAC 
GAGCAGGGGA GACTCGTGGT CAACTTCCGC ACAGAGGCCC GCTTCAGGGG TTTATTTGTA 
GTGGGGCATC CTGCAAATTT GAGGAGTCTG AAAACTACAG CGTCCACAGT GTCTACAGTG 
ATGAGTGCAG ATCACCCAGG CTTGACCTTC AACCTAACTC TGGTCAGGAC GGAGCCGACC 
TACAGCCAAC CCGTCCAGCA GTGGATGTTT ATCTCAGACT TCGCTGTTCG TGATTATTCT 
GGCACATACA CAGTGAGCTT GATCCCCTGT ACAGCTGCTC TGAATGTGGA GTTCACAGTT 
CCTCCAGTCT GCAACCCTAG AGAAGCCATC ACGTTTGATC TCGATATCCG CTTCCAGCAG 
GTGAGTGACC CTGTGGCTGT GGAGTTCAGT CTGAACACAC AGATGTTCCT GCTGTCTAAG 
AAAGTGCTGT GGCTGTCAGA TGGATCAATG GGCTTTGGTC AGGAAAGTGA CGCCGCTTTC 
TCTGAAGGTG ACGTGATCTA TGGACGTGTG ATGGTGGACC CGGTACAGAA TTTGGGCGAC 
TCTTTCTACT GTAACATTGA GAAGGTGTAT CTGTGCACTG GAGCTGATGG ATATGTTCCT 
AAATACAACC CCAGCAAGTC TGAGTTTGGA TGCCTTGCTG ACTCTCCGTC TCTGCTCTAC 
CAATTCAAGA TTATTGACAA AGCTCAGCCA GAAACTCAGG CCAGAAGTTT TGGGAATGTG 
GACTTCAATG CTCTGCTGGC TGTAGATGAC CCATCGGCCC TCTCTCTGGT CAAACAGCCT 
GGATCTGATG GCTTCAGACT CCATTCTACA CCCCTCTTCC AGGTGGCAGT AGGGAGAGAG 
TGGTTCATTC ACACCATCTA CACTGTGCGT TCAAAAGACA ACGCCAAACG TGGCATCGGC 
AAACGAAGTG TCGAACAGCA CTTGATAACA TCAGGAGACC TTAAAGAGTC AAGAAGACGC 
TCCAGACGCT CCAACAGAGA CGTTCCCACA CTGGCTGAGC AGATTGGCCC CGAAAACAAT 
CGAGGAACCA ACATCATGCA CATCGCTCTA GACCGCAGCC GCCGCACTGC ATCTGAAGAA 
GAGCTGTTTG CGGAGGGACT TTCTCCACGA GGACTCAGTG GACGACAGGC AGAAGAAGAA 
GCGGTGACGG TGGCAGGAGC TCTTGTGGGT TTGCTGCTCA CCGTTCTTGT GTTCGTAGCA 
TTAGCATTGG TGGTTCGCTC AAGGCGGACG CCAGCTAAAA AGGCCCCAAG AGTATCACCC 
AGCCTGCAGC CGGTCATTGT GCGGAGCAGT CTTGATAATG GGGACAGTTC AGAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem2a ( XM_005155380.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155437.1 Danio rerio Fras1 related extracellular matrix protein 2a (frem2a), transcript variant X1, mRNA.

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CloneID	ODa16167
Clone ID Related Accession (Same CDS sequence)	XM_005155380.3 , XM_005155380.4
Accession Version	XM_005155380.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	FRAS1-related extracellular matrix protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155380.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTTC ACAGATTTCT TCTCCTCCTG CTATCACTGA CAGGCAGTTC CATGAGCGCA 
CTTGAAACGC ACGGACTGTC GTACGCGTCC AAGCCTGAAC TCTCCGAGGA CGCTATACTG 
GTGGCAAACA ACGGCATACA TGTGCCTTTC GGGAGATCTG TTTACATAGA TCCGATCAAC 
GACTTGGTTA TTGAACTTCA GCCGGGAGAC AGGTGCACTA TAACAGTTCT GGATAATGAT 
CCGCTGTCTC AGAGACCGGG ACACGTGTCT CCTAAAAAGT TCCCGTGCGA ATTCGGTCCT 
AACGATGTGA GATACTCTCA TTTCGGTTCT AGGAGCCCTG CAGGGGACAG AGTGAAGCTG 
CAACTCAGGT ATGACACACA GACTGAAACT ATCATCATCC CCTTTATGCT GGAAGTGGAG 
GTTGTTTTCA CACAATTGGA GGTTGTTACT AAGAACATGC CTCTGGTTGT TGATAAACTA 
CATGGAACTA GTAATCCTAT TAATAAAAAG ATTTTAGATT TCACTTATGA AAAAGACTCT 
GAACAATGTG AGGTAACGTC ACTGGCTGCC AGCAGTGTTC TTCCTAAATA CGGTAAAGTT 
GTTGATGATA AAAAGCTGGA TAAAATGATG AGCTGTGAGT TGTTCCTCAA ATCAAATATC 
CGTTATGAGC ACACGTCTAG CCACAAGTCC CCTAATAAGG ATTACATCCC CATGGTTGTG 
GAGCTACGAA ACAAAGAAAA TAATTTGCTG AAACAGGAGT TTTTCCATGT GATGATCCGG 
ATCAAAGAGG GTGAAGAAAA CACTCCCCCT AGACCCAGTT TTGTGGCTAT GATGATGATG 
GAAGTTGATC AGTTTGTAAT GACGGCTTTA ACTCCTGATA TGTTGGCTGC TGAGGACACT 
GAATCCAATT CTGATGATCT CATTTTCAAC ATCACTTCTC CTCTGTCCAT TGAGGAGGGT 
TATATCGTGA GTACAGATGA CAGGAATTTA CCGATTACAT CCTTTTATCA AAAGGATCTG 
AAAGATCTGA AAATAGCATA CAAACCCCCC TCTTTGGACT CAGACACTGA GCGTATCTTT 
CAAATAGAGT TTGAGGTCAT CGACCCAGAT GGTGGCATTT CAGATCCTTT TGCTTTCATG 
ATCGTTGTGA AGCCCATGAA TACATTAGCG CCGATAGTCA CTAAAAATTC TGGCCAGCTT 
TTATATGAAG GCCAGTCTAG GTCTTTGTTT ACTCAAAACA ATTTAGAAAT TAGCGACGAA 
GACAACCTGG ACAGTGTGAG AATAACAGTG GTTGATGGGC TGCGTCATGG TGTCCTAACT 
GTTTTGGGCT CCAACAGAAA GTATTTTTCT CCGGCTGACT TGGAGGTTGG CATTGTGGTT 
TACCAGCATG ATGGCAGTGA CACATACAGT GATAATATAA TATTCAGAAT GACAGACGGG 
AAGAATGAAG TGGAGTTTTT GTTCCCCATC ACAATAGTAC CCACAGACGA TGAACCACCC 
ATCATAAATG CAAACACTGG ACTGATATTA TTCAAAAATC AGATGGTGCC CATCTCGCAG 
CTCATGCTCA GTGCTGCAGA TATTGACTCT GAAGATTCCA TTATTAAATT TACAATCGAG 
CCTCCCTTTT CAGCTATTGG CGAGGTCGTG TTGAGGCAAT CTGAAGCACC AGAGGATCCG 
TCATCCTGGA AATTTAATGC AGACGACGGT GTGTATGAGA AAGTAGTGAC AGAGTGGCTC 
CAGAAAGATA TTACAAATGA AAAGCTCTTC TATAGGCATA CGGGTCCACA TATCACAGAG 
ACTGTTACAG ATCAGTTTGT GTTTAAAGTG AAAGATGACG CTGATCCTCC CAACCAGTCC 
AGTGAGAATA ATTTTATTAT TAAAATTTTG CCTATTGATG ATATTCCACC CGTGCTGTTC 
CCAGGCACTA CTTTACAGAT GACAGTACAT GAGTACCAGC TGACTCATTT AAGCAAGAAT 
GTTCTTCGTT ACACCGACTT GGACTCAGAG GACCGGGACC TGAAATACAC AATAACTCAA 
GCACCAACTG ATATGGATGA AAACAATCCT GTCAAATTTG GCTCTATTGT TTTAACTGAC 
AGCCCCGATA CTGATGTAAC CGAATTTACC CAAGCGCAAA TTAACCATCA TAAGATTTCC 
TACAACCCGC CAGACCTTGA ACTCGGCATC ATACCTCATG TGGTGCAGTT CCATTTCACA 
GTAGAGGACA CTGCTGGAAA TGGTGCTGAT GGAGTTTTTA CAATCTTTCT GCAGCCTGTA 
AACAACAAAC CCCCACAGAT AACAAACTCT GGATTCACAG TCCTTGAGAG AGGGGTGCAT 
GTTATTACGA GCGCCGAACT TGACGCCAAT GACCTTGATA CTGACATTAA GCTAATATCA 
TTCACGCTAA GTCAAACTCC ACTGCACGGT CATCTTCAAC ATACGTTTAC TGACTTAAAA 
AAAGGGAGTC ATTTCAACTT GATGGATATT TCTGATGGTC AGGTGTCATA TGTCCACAAT 
GGGGATGAAA CGACAAGTGA CTCTTTTAAA CTAGATGTTA GTGACGGTGT TCATGTTGTA 
ACGATCACTG TGAAGGTAAC TGTAAAGCTT ATTGATGATG AAACACCAAC ACTTAGCCTC 
ACAAAGGGCA CCTTTGGCTC TAGATTGGAT GTGCTGGAAA ATGGGGCTAC AGAAATTACT 
CCCAATGTCA TTCAGGGGAA AGATGAGGAC ACTGATGACC TGCAGTTGAC ATTCATTGTG 
GAAGAAGCAC CTAAGTTTGG AGAAATTCTG GTGAATGGAG TTTCCTCGAG CAGATTCACC 
CAAGCTGACA TCATCAGTGG TCTTGTTGTG TATGCGCACA CCAGTGGTGA GATTGGTCTT 
ACTTCAATTG AAGATTTTTT CAGTTTGACC CTCTCTGACA TGTCTGATGA GTGGAATGTA 
GGGGGTAACC GAGTACACGG AGTCCGTGTT CATGTCACCA TTCTCCCTGT AGATAACCAG 
ACTCCATTGG TCACAGTTGG CCCTACTTTT AATGTCTTTG AAGCAGATAA AAACATTATC 
GGGAGCCATC AGATTAAAGT CGAGGATGCC GACACACCAA ACGAGGACAT CCTGTGCACC 
ATTATCGTTC AGCCCACATC AGGATATGTG GAGAACATTT CTCCAGCTCC AGGCTCTGAA 
AAGTCAAGAG CAGGAACGGC AGTGAGTGCT TTCACCATCA GTGATGTGAA TGAAGGTCAT 
ATTTATTATG TCCAAAGCAT ACACAAAGGA GTTGAGCCAG TGGAGGACAG GTTCACGTTC 
AGATGTTCTG ATGGTGTGAA TTTCTCAGAG AGACAGTTCT TTCCCATTGT CATTATCCCC 
ACAAATGATG AAAAACCAGA AATCTTCATG CGAGAGGTTG TTGTAATGGA GGGTATGAAT 
GTTATTATTG ACACGCCACT CTTGAATGGA GTCGATGCTG ATGTTCCTGC TCAAGAGTTG 
ATGTTTATTA TCACCAAACT TCCCAATCAT GGGTTCATTC TCAATCAGCT CGCCAGTGGC 
TCTGTCCCAG TCACAAACTT CACTCTGGAA CAAATCAAAG AAGCCTCTAG CATAATTTAC 
GAACATGATG ATTCTGAAAC CACCGAAGAC AAGTTTGATG TTCTTCTTAC AGATGGGAAA 
CATAAAGCTG AAGGCACAGT CATGATCATG ATTATTCCAG TGGATGATGA AACACCAAGG 
CTTGCCATAA ATGACGGGCT TGAAATTGAG ATTGGTGAAA CAAAAGAGAT AAATGCTAAA 
GTGCTGAAAG CTACTGATTT GGACTCAGAC GACGAGTCCC TTACTTTCAT TATTCGCTAC 
GGCCCAGCTC AAGGCTTACT GTATAGAAAA TCAGCAGGAT CTGTGGAAAA GAGATCTGCT 
GGATCTACAG AGAACATAAC AGTTGGCATG AACTTTACAC AAAATGAGGT GAACAGAGGG 
CTCATTTTGT ACACACACAA TGGACAGGAA GGCATGCGTG ATCTTGTAAA GTTTGATGTA 
ACTGACGGCA TCAATCCCCT AATTGACAGA TACTTCTATA TCACTGTGGG CAGCATTGAC 
ATGGTGTTTC CTGATGTCAT CAGTAAGGGG GTGTCTCTTA AAGAAGGTGG CAAAGTGACA 
CTCACTACGA ACCTCCTCAG CACTAGTGAT CTCAACAGCC CCGATGAGAA TTTAGTCTTT 
ACTATCACAA GAGCCCCAGT GAGAGGACAT TTGGAAAGCA CGGACACACC AGGCATGCCT 
ATAGTATCCT TTACCCAATT ACAACTGGCC GGGAGCAAGA TATACTACAT CCATACTGCT 
GATGATGAAG TCAAGATGGA CAGCTTTGAG TTTGAGGTGA CCGATGGTTA CAACCCTGTG 
TTTCGCACAT TTCGCGTTTC AATCGTTGAT GTTGACAATA AAAAGCCAGT ATTGACCATT 
CATGACCTTC TAGTAACGGA GGGACAAACC AAACTCATTA CACCCTTTGA GTTGACCGTT 
GAGGACCAAG ACACTGCCGA GCACCTTCTG AAGTTTAAAG TCTCCCAATT GCCCATTCAT 
GGGAAACTAC TTTTCAACAA CAGCCAGCCA TTGACTTCTT TCACAAAGCA AGACCTAAAT 
GAAAACATGA TCAGTTACAA GCATGATGGA ACGGAGTCGA AAAAAGATAG CTTTTCATTT 
ACAGTCACCG ATGGTACCCA TTCAGACTTT TTTGTATTCC CTGATACAAT TTTTGAGACC 
CAAAGGCCTC AGACCATGAA GATCAACATT GTGTCTGTGG ACAATGGCGT GCCTCAGATT 
GAGGTTAACA AAGGTGCACC AACTTTAAAA GTATTGTTCG CTGGACACTT GGGTTATCAG 
ATGACAAGCA AAGTCCTGAA GGCAGTGGAC AGAGACAGCA ACCCAGAGGC TTTAATTTTT 
CACATTACCA CACAGCCAGA GCATGGTTAT CTCATTAGCC TGGACAGAGG GACGGAGAAT 
GACTCTATCA ACACCTTCAC ACAAGCTGAC ATAAATGATC TTAAGATCTG CTATATCCTG 
AAGGACGAGA ACAATGCCAC CAGTGACGTC TTTAGTTTTA CCGTGGAGGA TAATGGAGGG 
AACAAACTGA AGAACCAACC GTTTCATCTG AACTGGTCTT GGATCTCTCT GGAGAAGCCA 
AACTATGTGG TGGATGAGCA CGACAAAGTT CTGGAGGTGA CCCTCAGACG GAGAGGTTTC 
CTGGGTGAAA CGTCTTTTGT TGGGATTGGT ACCTTGGATG GCAGTGCAGA GAAGGAGAAG 
GATTTCCTGG GAAAATCGCA GAAGCAGGTG CAGTTTAACC CTGGCCAGAC CACAGCCTCC 
TGGAAAGTCA GAGTTCTCTC GGATGGGAAA TACGAGCAGT CCGAAACGTT TCAAATTGTG 
CTGACTGAGC CGGTGATGGG CGTTTTGGAG GCGCCAGCTG TAGCCACAGT TGAGATTGTG 
GATCCAGGTG ATGAGTCCAT GGTGTTCTTT CCTCAGGCCG GCTTTGCTGT GGAAGAAGAT 
ATTGGAGAGC TGCTTGTCCC AGTGCGCAGG ACTGGAGACG TGAGCCAGGA GCTCATGGTG 
GTCTGTCACA CACAGCAAGA CTCTGCCACA GGAACTGTCC CCACATCGGT TCTGTCCTAC 
TCAGACTACA TCTCGCGTCC AGAGGAGCAG GTCAGCATCC TGCGCTTTGA TAAGGACGAG 
ACGGAAAAAC ACTGTCGTGT GGTGATCATC GATGACTCTC TGTATGAGAG CGAGGAGAGC 
TTCAACGTGA CCCTCAGCAT GCCTATAGGA GGCCGTCTGG GGCCCGAATA CCCCACTGTG 
AATATCCGCA TTCTGCCCGA CCAGGATGAT GCTCCGGTGT TCTACTTTGG CAGTCAGGAG 
TATCATGTGG ATGAGAGTGA CGGTCATGTT GAAGTGCGTG TGTGGAGAAC GGGCACAGAT 
CTCTCTAAAA CGGCAACGGT CACTGTGAGA TCCCGCAAAA CAGACCCTGT TTCTGCTGAA 
GCTGGAGTGG ACTATGTGGG AATCAGCAGA AACTTAGACT TCGCTCCGGG TGTAAGCATG 
CAGACGGTGC GGGTCACGGT GCTGGATGAC CTCGGACAGC CTGTTCTGGA AGGGACAGAA 
AGATTTGAGC TTGTTCTCCG GATGCCCTCC AATGGAATTC TTGGAGAGCC GGCCAAAGTC 
ACAGTCTTTA TCAACGACTC CATTTCAGAC CTGCCAAAGG TGCAGTTTAA ACAGGCTGTC 
GTGACTGTAG AGGAGAGTGA AGGTCATGTC ACTGTAATGG TGCACCGCAG TGGAGACATT 
AAACACAAGT CCAGTGTCCG CTGCTACACG CGGCAGGGCT CCGCACAGGT GGTATACGAC 
TTTGAGGAGA GACCAAACAC TGACGCCTCC ATCGTAACTT TCTTGCCTGG TGAAGTTGAT 
AAACCTTGTG TCTTGAAACT GGTGGATGAT TCAATTCATG AGGAGGAGGA AGAACTGAGG 
CTCGTTTTGG GAAATGCAAA GAGTGATTCG CCATACGGAG CGTCCATTGG AAACCAGAAT 
GAAACACTCA TAAAGATCAA GGACAATGCT GACAAATCCA TCATAAAGTT TGCCGAGACG 
AAGTTCAGTG TTAGCGAACC GAAAGAGTCT GGGCAGGTGA CATTAGTAAA GATCCCCATC 
CGCAGAACAG GAGACTCTTC CAAAGTGTCC CTAGTGAGGG TGCACACTAA AGACGGCTCT 
GCCATATCCG GAGAAGACTA TCATCCTATC TCCCTCGATG TGGAGTTTAA AGCAGAAGAG 
ATCGAGCATG TAGTTGAAGT GGAGGTTTTG TTTGATGGAA TAAGAGAGAT GAGAGAGGCC 
TTCACTGTCC ATTTAAAACC AGATGAGAAC ATGATTGCTG AAATACAGAT GACAAAAGCA 
ATAGTGTACA TCGAGGAGAC CAACAGCATG GCTGATGTCA CATTCCCTTC ATTGCCACAT 
ATTGTGTCAC TGCTGCACTA TGACAATATC AGAACAAGCA AACAAAGTGC TCATCCACCC 
GCTGGATATC CTGTCGTCTG TGTCACGGCC TGCAACCCCA AATATCCAGA CTACGACAAA 
ACAGGCTCCA TCTGCATTAG CGAACGCATC AACAACACGC TGACTCGTTA CCGATGGCTC 
ATTAGCGCCC CCAGCGGTCC TGATGGCGTC ACCAGCCCCA TGAGGGAGGC TGACTTCGAC 
ACCTTCTTCA CATCCTCCAA AATGATCACC CTAGACTCCA TTTACTTCCA AGCGGGCTCC 
AGAGTGCAGT GTGCAGCCCG TGCCGTGAAT TCAAATGGAG ACGAGGGTTT GGAACTGAGC 
AGCCCAATAG TGTCTATTAG CATGGAGGAA GGGATGTGCC AACCACGAAA ATTGGGCACA 
GTGGGTGCTG AGCCCTTTTC TGCCAAACTA CGTTATACGG GCGCTGAAGA CCTTGAATAT 
GCCAATCTCA TCAAGCTTAC TGTCACCATG CCCCACATAG ACGGCATGCT CCCCGTGGTC 
TCCACTCGTG CTCTGTCCAA TTTTGACCTG ACTCTGAGCC CCGATGGCAC TCGCGTGGGA 
AACCACAGGT GCTCCAATCT GCTGGACTTT AATGAGGTGT CCACACGGCA CGGCTTCCTG 
ACCAAAGCCA CCCGCAATAT CCAGAGCGTT GGAGAGACCC TACCTTATCA GTACAGCCCT 
GTCCTCCGAG GCCAGGGCAC TTTACGCTTC TACAGGAACC TGAACCTGGA GGCCTGTCTG 
TGGGAGTTTG TGAGTTATTA TGACATGTCA GAGCTGCTCA CAGACTGCGG GGGAACCATC 
GGGACAGATG GCCAGGTGCT GAACCTGGTG CAGTCATACG TGACCCTGCG TGTTCCTCTT 
CACGTCTCAT ACGTGTTTCA TTCCCCTGTG GGTGCTGGAG GCTGGCAGCA TTTCGACCTG 
CACTCCGAGC TCCGGCTCAC CTTTGTTTAT GACACTGCCA TCTTGTGGAA GGACGGAGTC 
GGCAGCCAAA CACAGGCTGA TCTACAAGGA GCTTTATATC CCACCAGCAT GAGGATTAAC 
GAGCAGGGGA GACTCGTGGT CAACTTCCGC ACAGAGGCCC GCTTCAGGGG TTTATTTGTA 
GTGGGGCATC CTGCAAATTT GAGGAGTCTG AAAACTACAG CGTCCACAGT GTCTACAGTG 
ATGAGTGCAG ATCACCCAGG CTTGACCTTC AACCTAACTC TGGTCAGGAC GGAGCCGACC 
TACAGCCAAC CCGTCCAGCA GTGGATGTTT ATCTCAGACT TCGCTGTTCG TGATTATTCT 
GGCACATACA CAGTGAGCTT GATCCCCTGT ACAGCTGCTC TGAATGTGGA GTTCACAGTT 
CCTCCAGTCT GCAACCCTAG AGAAGCCATC ACGTTTGATC TCGATATCCG CTTCCAGCAG 
GTGAGTGACC CTGTGGCTGT GGAGTTCAGT CTGAACACAC AGATGTTCCT GCTGTCTAAG 
AAAGTGCTGT GGCTGTCAGA TGGATCAATG GGCTTTGGTC AGGAAAGTGA CGCCGCTTTC 
TCTGAAGGTG ACGTGATCTA TGGACGTGTG ATGGTGGACC CGGTACAGAA TTTGGGCGAC 
TCTTTCTACT GTAACATTGA GAAGGTGTAT CTGTGCACTG GAGCTGATGG ATATGTTCCT 
AAATACAACC CCAGCAAGTC TGAGTTTGGA TGCCTTGCTG ACTCTCCGTC TCTGCTCTAC 
CAATTCAAGA TTATTGACAA AGCTCAGCCA GAAACTCAGG CCAGAAGTTT TGGGAATGTG 
GACTTCAATG CTCTGCTGGC TGTAGATGAC CCATCGGCCC TCTCTCTGGT CAAACAGCCT 
GGATCTGATG GCTTCAGACT CCATTCTACA CCCCTCTTCC AGGTGGCAGT AGGGAGAGAG 
TGGTTCATTC ACACCATCTA CACTGTGCGT TCAAAAGACA ACGCCAAACG TGGCATCGGC 
AAACGAAGTG TCGAACAGCA CTTGATAACA TCAGGAGACC TTAAAGAGTC AAGAAGACGC 
TCCAGACGCT CCAACAGAGA CGTTCCCACA CTGGCTGAGC AGATTGGCCC CGAAAACAAT 
CGAGGAACCA ACATCATGCA CATCGCTCTA GACCGCAGCC GCCGCACTGC ATCTGAAGAA 
GAGCTGTTTG CGGAGGGACT TTCTCCACGA GGACTCAGTG GACGACAGGC AGAAGAAGAA 
GCGGTGACGG TGGCAGGAGC TCTTGTGGGT TTGCTGCTCA CCGTTCTTGT GTTCGTAGCA 
TTAGCATTGG TGGTTCGCTC AAGGCGGACG CCAGCTAAAA AGGCCCCAAG AGTATCACCC 
AGCCTGCAGC CGGTCATTGT GCGGAGCAGT CTTGATAATG GGGACAGTTC AGAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155437.1
CDS	255..9614
Translation	MELHRFLLLLLSLTGSSMSALETHGLSYASKPELSEDAILVANNGIHVPFGRSVYIDPINDLVIELQPGDRCTITVLDNDPLSQRPGHVSPKKFPCEFGPNDVRYSHFGSRSPAGDRVKLQLRYDTQTETIIIPFMLEVEVVFTQLEVVTKNMPLVVDKLHGTSNPINKKILDFTYEKDSEQCEVTSLAASSVLPKYGKVVDDKKLDKMMSCELFLKSNIRYEHTSSHKSPNKDYIPMVVELRNKENNLLKQEFFHVMIRIKEGEENTPPRPSFVAMMMMEVDQFVMTALTPDMLAAEDTESNSDDLIFNITSPLSIEEGYIVSTDDRNLPITSFYQKDLKDLKIAYKPPSLDSDTERIFQIEFEVIDPDGGISDPFAFMIVVKPMNTLAPIVTKNSGQLLYEGQSRSLFTQNNLEISDEDNLDSVRITVVDGLRHGVLTVLGSNRKYFSPADLEVGIVVYQHDGSDTYSDNIIFRMTDGKNEVEFLFPITIVPTDDEPPIINANTGLILFKNQMVPISQLMLSAADIDSEDSIIKFTIEPPFSAIGEVVLRQSEAPEDPSSWKFNADDGVYEKVVTEWLQKDITNEKLFYRHTGPHITETVTDQFVFKVKDDADPPNQSSENNFIIKILPIDDIPPVLFPGTTLQMTVHEYQLTHLSKNVLRYTDLDSEDRDLKYTITQAPTDMDENNPVKFGSIVLTDSPDTDVTEFTQAQINHHKISYNPPDLELGIIPHVVQFHFTVEDTAGNGADGVFTIFLQPVNNKPPQITNSGFTVLERGVHVITSAELDANDLDTDIKLISFTLSQTPLHGHLQHTFTDLKKGSHFNLMDISDGQVSYVHNGDETTSDSFKLDVSDGVHVVTITVKVTVKLIDDETPTLSLTKGTFGSRLDVLENGATEITPNVIQGKDEDTDDLQLTFIVEEAPKFGEILVNGVSSSRFTQADIISGLVVYAHTSGEIGLTSIEDFFSLTLSDMSDEWNVGGNRVHGVRVHVTILPVDNQTPLVTVGPTFNVFEADKNIIGSHQIKVEDADTPNEDILCTIIVQPTSGYVENISPAPGSEKSRAGTAVSAFTISDVNEGHIYYVQSIHKGVEPVEDRFTFRCSDGVNFSERQFFPIVIIPTNDEKPEIFMREVVVMEGMNVIIDTPLLNGVDADVPAQELMFIITKLPNHGFILNQLASGSVPVTNFTLEQIKEASSIIYEHDDSETTEDKFDVLLTDGKHKAEGTVMIMIIPVDDETPRLAINDGLEIEIGETKEINAKVLKATDLDSDDESLTFIIRYGPAQGLLYRKSAGSVEKRSAGSTENITVGMNFTQNEVNRGLILYTHNGQEGMRDLVKFDVTDGINPLIDRYFYITVGSIDMVFPDVISKGVSLKEGGKVTLTTNLLSTSDLNSPDENLVFTITRAPVRGHLESTDTPGMPIVSFTQLQLAGSKIYYIHTADDEVKMDSFEFEVTDGYNPVFRTFRVSIVDVDNKKPVLTIHDLLVTEGQTKLITPFELTVEDQDTAEHLLKFKVSQLPIHGKLLFNNSQPLTSFTKQDLNENMISYKHDGTESKKDSFSFTVTDGTHSDFFVFPDTIFETQRPQTMKINIVSVDNGVPQIEVNKGAPTLKVLFAGHLGYQMTSKVLKAVDRDSNPEALIFHITTQPEHGYLISLDRGTENDSINTFTQADINDLKICYILKDENNATSDVFSFTVEDNGGNKLKNQPFHLNWSWISLEKPNYVVDEHDKVLEVTLRRRGFLGETSFVGIGTLDGSAEKEKDFLGKSQKQVQFNPGQTTASWKVRVLSDGKYEQSETFQIVLTEPVMGVLEAPAVATVEIVDPGDESMVFFPQAGFAVEEDIGELLVPVRRTGDVSQELMVVCHTQQDSATGTVPTSVLSYSDYISRPEEQVSILRFDKDETEKHCRVVIIDDSLYESEESFNVTLSMPIGGRLGPEYPTVNIRILPDQDDAPVFYFGSQEYHVDESDGHVEVRVWRTGTDLSKTATVTVRSRKTDPVSAEAGVDYVGISRNLDFAPGVSMQTVRVTVLDDLGQPVLEGTERFELVLRMPSNGILGEPAKVTVFINDSISDLPKVQFKQAVVTVEESEGHVTVMVHRSGDIKHKSSVRCYTRQGSAQVVYDFEERPNTDASIVTFLPGEVDKPCVLKLVDDSIHEEEEELRLVLGNAKSDSPYGASIGNQNETLIKIKDNADKSIIKFAETKFSVSEPKESGQVTLVKIPIRRTGDSSKVSLVRVHTKDGSAISGEDYHPISLDVEFKAEEIEHVVEVEVLFDGIREMREAFTVHLKPDENMIAEIQMTKAIVYIEETNSMADVTFPSLPHIVSLLHYDNIRTSKQSAHPPAGYPVVCVTACNPKYPDYDKTGSICISERINNTLTRYRWLISAPSGPDGVTSPMREADFDTFFTSSKMITLDSIYFQAGSRVQCAARAVNSNGDEGLELSSPIVSISMEEGMCQPRKLGTVGAEPFSAKLRYTGAEDLEYANLIKLTVTMPHIDGMLPVVSTRALSNFDLTLSPDGTRVGNHRCSNLLDFNEVSTRHGFLTKATRNIQSVGETLPYQYSPVLRGQGTLRFYRNLNLEACLWEFVSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLHVSYVFHSPVGAGGWQHFDLHSELRLTFVYDTAILWKDGVGSQTQADLQGALYPTSMRINEQGRLVVNFRTEARFRGLFVVGHPANLRSLKTTASTVSTVMSADHPGLTFNLTLVRTEPTYSQPVQQWMFISDFAVRDYSGTYTVSLIPCTAALNVEFTVPPVCNPREAITFDLDIRFQQVSDPVAVEFSLNTQMFLLSKKVLWLSDGSMGFGQESDAAFSEGDVIYGRVMVDPVQNLGDSFYCNIEKVYLCTGADGYVPKYNPSKSEFGCLADSPSLLYQFKIIDKAQPETQARSFGNVDFNALLAVDDPSALSLVKQPGSDGFRLHSTPLFQVAVGREWFIHTIYTVRSKDNAKRGIGKRSVEQHLITSGDLKESRRRSRRSNRDVPTLAEQIGPENNRGTNIMHIALDRSRRTASEEELFAEGLSPRGLSGRQAEEEAVTVAGALVGLLLTVLVFVALALVVRSRRTPAKKAPRVSPSLQPVIVRSSLDNGDSSEV

Target ORF information:

RefSeq Version	XM_005155380.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Fras1 related extracellular matrix protein 2a (frem2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155380.4

ORF Insert Sequence:

ATGGAACTTC ACAGATTTCT TCTCCTCCTG CTATCACTGA CAGGCAGTTC CATGAGCGCA 
CTTGAAACGC ACGGACTGTC GTACGCGTCC AAGCCTGAAC TCTCCGAGGA CGCTATACTG 
GTGGCAAACA ACGGCATACA TGTGCCTTTC GGGAGATCTG TTTACATAGA TCCGATCAAC 
GACTTGGTTA TTGAACTTCA GCCGGGAGAC AGGTGCACTA TAACAGTTCT GGATAATGAT 
CCGCTGTCTC AGAGACCGGG ACACGTGTCT CCTAAAAAGT TCCCGTGCGA ATTCGGTCCT 
AACGATGTGA GATACTCTCA TTTCGGTTCT AGGAGCCCTG CAGGGGACAG AGTGAAGCTG 
CAACTCAGGT ATGACACACA GACTGAAACT ATCATCATCC CCTTTATGCT GGAAGTGGAG 
GTTGTTTTCA CACAATTGGA GGTTGTTACT AAGAACATGC CTCTGGTTGT TGATAAACTA 
CATGGAACTA GTAATCCTAT TAATAAAAAG ATTTTAGATT TCACTTATGA AAAAGACTCT 
GAACAATGTG AGGTAACGTC ACTGGCTGCC AGCAGTGTTC TTCCTAAATA CGGTAAAGTT 
GTTGATGATA AAAAGCTGGA TAAAATGATG AGCTGTGAGT TGTTCCTCAA ATCAAATATC 
CGTTATGAGC ACACGTCTAG CCACAAGTCC CCTAATAAGG ATTACATCCC CATGGTTGTG 
GAGCTACGAA ACAAAGAAAA TAATTTGCTG AAACAGGAGT TTTTCCATGT GATGATCCGG 
ATCAAAGAGG GTGAAGAAAA CACTCCCCCT AGACCCAGTT TTGTGGCTAT GATGATGATG 
GAAGTTGATC AGTTTGTAAT GACGGCTTTA ACTCCTGATA TGTTGGCTGC TGAGGACACT 
GAATCCAATT CTGATGATCT CATTTTCAAC ATCACTTCTC CTCTGTCCAT TGAGGAGGGT 
TATATCGTGA GTACAGATGA CAGGAATTTA CCGATTACAT CCTTTTATCA AAAGGATCTG 
AAAGATCTGA AAATAGCATA CAAACCCCCC TCTTTGGACT CAGACACTGA GCGTATCTTT 
CAAATAGAGT TTGAGGTCAT CGACCCAGAT GGTGGCATTT CAGATCCTTT TGCTTTCATG 
ATCGTTGTGA AGCCCATGAA TACATTAGCG CCGATAGTCA CTAAAAATTC TGGCCAGCTT 
TTATATGAAG GCCAGTCTAG GTCTTTGTTT ACTCAAAACA ATTTAGAAAT TAGCGACGAA 
GACAACCTGG ACAGTGTGAG AATAACAGTG GTTGATGGGC TGCGTCATGG TGTCCTAACT 
GTTTTGGGCT CCAACAGAAA GTATTTTTCT CCGGCTGACT TGGAGGTTGG CATTGTGGTT 
TACCAGCATG ATGGCAGTGA CACATACAGT GATAATATAA TATTCAGAAT GACAGACGGG 
AAGAATGAAG TGGAGTTTTT GTTCCCCATC ACAATAGTAC CCACAGACGA TGAACCACCC 
ATCATAAATG CAAACACTGG ACTGATATTA TTCAAAAATC AGATGGTGCC CATCTCGCAG 
CTCATGCTCA GTGCTGCAGA TATTGACTCT GAAGATTCCA TTATTAAATT TACAATCGAG 
CCTCCCTTTT CAGCTATTGG CGAGGTCGTG TTGAGGCAAT CTGAAGCACC AGAGGATCCG 
TCATCCTGGA AATTTAATGC AGACGACGGT GTGTATGAGA AAGTAGTGAC AGAGTGGCTC 
CAGAAAGATA TTACAAATGA AAAGCTCTTC TATAGGCATA CGGGTCCACA TATCACAGAG 
ACTGTTACAG ATCAGTTTGT GTTTAAAGTG AAAGATGACG CTGATCCTCC CAACCAGTCC 
AGTGAGAATA ATTTTATTAT TAAAATTTTG CCTATTGATG ATATTCCACC CGTGCTGTTC 
CCAGGCACTA CTTTACAGAT GACAGTACAT GAGTACCAGC TGACTCATTT AAGCAAGAAT 
GTTCTTCGTT ACACCGACTT GGACTCAGAG GACCGGGACC TGAAATACAC AATAACTCAA 
GCACCAACTG ATATGGATGA AAACAATCCT GTCAAATTTG GCTCTATTGT TTTAACTGAC 
AGCCCCGATA CTGATGTAAC CGAATTTACC CAAGCGCAAA TTAACCATCA TAAGATTTCC 
TACAACCCGC CAGACCTTGA ACTCGGCATC ATACCTCATG TGGTGCAGTT CCATTTCACA 
GTAGAGGACA CTGCTGGAAA TGGTGCTGAT GGAGTTTTTA CAATCTTTCT GCAGCCTGTA 
AACAACAAAC CCCCACAGAT AACAAACTCT GGATTCACAG TCCTTGAGAG AGGGGTGCAT 
GTTATTACGA GCGCCGAACT TGACGCCAAT GACCTTGATA CTGACATTAA GCTAATATCA 
TTCACGCTAA GTCAAACTCC ACTGCACGGT CATCTTCAAC ATACGTTTAC TGACTTAAAA 
AAAGGGAGTC ATTTCAACTT GATGGATATT TCTGATGGTC AGGTGTCATA TGTCCACAAT 
GGGGATGAAA CGACAAGTGA CTCTTTTAAA CTAGATGTTA GTGACGGTGT TCATGTTGTA 
ACGATCACTG TGAAGGTAAC TGTAAAGCTT ATTGATGATG AAACACCAAC ACTTAGCCTC 
ACAAAGGGCA CCTTTGGCTC TAGATTGGAT GTGCTGGAAA ATGGGGCTAC AGAAATTACT 
CCCAATGTCA TTCAGGGGAA AGATGAGGAC ACTGATGACC TGCAGTTGAC ATTCATTGTG 
GAAGAAGCAC CTAAGTTTGG AGAAATTCTG GTGAATGGAG TTTCCTCGAG CAGATTCACC 
CAAGCTGACA TCATCAGTGG TCTTGTTGTG TATGCGCACA CCAGTGGTGA GATTGGTCTT 
ACTTCAATTG AAGATTTTTT CAGTTTGACC CTCTCTGACA TGTCTGATGA GTGGAATGTA 
GGGGGTAACC GAGTACACGG AGTCCGTGTT CATGTCACCA TTCTCCCTGT AGATAACCAG 
ACTCCATTGG TCACAGTTGG CCCTACTTTT AATGTCTTTG AAGCAGATAA AAACATTATC 
GGGAGCCATC AGATTAAAGT CGAGGATGCC GACACACCAA ACGAGGACAT CCTGTGCACC 
ATTATCGTTC AGCCCACATC AGGATATGTG GAGAACATTT CTCCAGCTCC AGGCTCTGAA 
AAGTCAAGAG CAGGAACGGC AGTGAGTGCT TTCACCATCA GTGATGTGAA TGAAGGTCAT 
ATTTATTATG TCCAAAGCAT ACACAAAGGA GTTGAGCCAG TGGAGGACAG GTTCACGTTC 
AGATGTTCTG ATGGTGTGAA TTTCTCAGAG AGACAGTTCT TTCCCATTGT CATTATCCCC 
ACAAATGATG AAAAACCAGA AATCTTCATG CGAGAGGTTG TTGTAATGGA GGGTATGAAT 
GTTATTATTG ACACGCCACT CTTGAATGGA GTCGATGCTG ATGTTCCTGC TCAAGAGTTG 
ATGTTTATTA TCACCAAACT TCCCAATCAT GGGTTCATTC TCAATCAGCT CGCCAGTGGC 
TCTGTCCCAG TCACAAACTT CACTCTGGAA CAAATCAAAG AAGCCTCTAG CATAATTTAC 
GAACATGATG ATTCTGAAAC CACCGAAGAC AAGTTTGATG TTCTTCTTAC AGATGGGAAA 
CATAAAGCTG AAGGCACAGT CATGATCATG ATTATTCCAG TGGATGATGA AACACCAAGG 
CTTGCCATAA ATGACGGGCT TGAAATTGAG ATTGGTGAAA CAAAAGAGAT AAATGCTAAA 
GTGCTGAAAG CTACTGATTT GGACTCAGAC GACGAGTCCC TTACTTTCAT TATTCGCTAC 
GGCCCAGCTC AAGGCTTACT GTATAGAAAA TCAGCAGGAT CTGTGGAAAA GAGATCTGCT 
GGATCTACAG AGAACATAAC AGTTGGCATG AACTTTACAC AAAATGAGGT GAACAGAGGG 
CTCATTTTGT ACACACACAA TGGACAGGAA GGCATGCGTG ATCTTGTAAA GTTTGATGTA 
ACTGACGGCA TCAATCCCCT AATTGACAGA TACTTCTATA TCACTGTGGG CAGCATTGAC 
ATGGTGTTTC CTGATGTCAT CAGTAAGGGG GTGTCTCTTA AAGAAGGTGG CAAAGTGACA 
CTCACTACGA ACCTCCTCAG CACTAGTGAT CTCAACAGCC CCGATGAGAA TTTAGTCTTT 
ACTATCACAA GAGCCCCAGT GAGAGGACAT TTGGAAAGCA CGGACACACC AGGCATGCCT 
ATAGTATCCT TTACCCAATT ACAACTGGCC GGGAGCAAGA TATACTACAT CCATACTGCT 
GATGATGAAG TCAAGATGGA CAGCTTTGAG TTTGAGGTGA CCGATGGTTA CAACCCTGTG 
TTTCGCACAT TTCGCGTTTC AATCGTTGAT GTTGACAATA AAAAGCCAGT ATTGACCATT 
CATGACCTTC TAGTAACGGA GGGACAAACC AAACTCATTA CACCCTTTGA GTTGACCGTT 
GAGGACCAAG ACACTGCCGA GCACCTTCTG AAGTTTAAAG TCTCCCAATT GCCCATTCAT 
GGGAAACTAC TTTTCAACAA CAGCCAGCCA TTGACTTCTT TCACAAAGCA AGACCTAAAT 
GAAAACATGA TCAGTTACAA GCATGATGGA ACGGAGTCGA AAAAAGATAG CTTTTCATTT 
ACAGTCACCG ATGGTACCCA TTCAGACTTT TTTGTATTCC CTGATACAAT TTTTGAGACC 
CAAAGGCCTC AGACCATGAA GATCAACATT GTGTCTGTGG ACAATGGCGT GCCTCAGATT 
GAGGTTAACA AAGGTGCACC AACTTTAAAA GTATTGTTCG CTGGACACTT GGGTTATCAG 
ATGACAAGCA AAGTCCTGAA GGCAGTGGAC AGAGACAGCA ACCCAGAGGC TTTAATTTTT 
CACATTACCA CACAGCCAGA GCATGGTTAT CTCATTAGCC TGGACAGAGG GACGGAGAAT 
GACTCTATCA ACACCTTCAC ACAAGCTGAC ATAAATGATC TTAAGATCTG CTATATCCTG 
AAGGACGAGA ACAATGCCAC CAGTGACGTC TTTAGTTTTA CCGTGGAGGA TAATGGAGGG 
AACAAACTGA AGAACCAACC GTTTCATCTG AACTGGTCTT GGATCTCTCT GGAGAAGCCA 
AACTATGTGG TGGATGAGCA CGACAAAGTT CTGGAGGTGA CCCTCAGACG GAGAGGTTTC 
CTGGGTGAAA CGTCTTTTGT TGGGATTGGT ACCTTGGATG GCAGTGCAGA GAAGGAGAAG 
GATTTCCTGG GAAAATCGCA GAAGCAGGTG CAGTTTAACC CTGGCCAGAC CACAGCCTCC 
TGGAAAGTCA GAGTTCTCTC GGATGGGAAA TACGAGCAGT CCGAAACGTT TCAAATTGTG 
CTGACTGAGC CGGTGATGGG CGTTTTGGAG GCGCCAGCTG TAGCCACAGT TGAGATTGTG 
GATCCAGGTG ATGAGTCCAT GGTGTTCTTT CCTCAGGCCG GCTTTGCTGT GGAAGAAGAT 
ATTGGAGAGC TGCTTGTCCC AGTGCGCAGG ACTGGAGACG TGAGCCAGGA GCTCATGGTG 
GTCTGTCACA CACAGCAAGA CTCTGCCACA GGAACTGTCC CCACATCGGT TCTGTCCTAC 
TCAGACTACA TCTCGCGTCC AGAGGAGCAG GTCAGCATCC TGCGCTTTGA TAAGGACGAG 
ACGGAAAAAC ACTGTCGTGT GGTGATCATC GATGACTCTC TGTATGAGAG CGAGGAGAGC 
TTCAACGTGA CCCTCAGCAT GCCTATAGGA GGCCGTCTGG GGCCCGAATA CCCCACTGTG 
AATATCCGCA TTCTGCCCGA CCAGGATGAT GCTCCGGTGT TCTACTTTGG CAGTCAGGAG 
TATCATGTGG ATGAGAGTGA CGGTCATGTT GAAGTGCGTG TGTGGAGAAC GGGCACAGAT 
CTCTCTAAAA CGGCAACGGT CACTGTGAGA TCCCGCAAAA CAGACCCTGT TTCTGCTGAA 
GCTGGAGTGG ACTATGTGGG AATCAGCAGA AACTTAGACT TCGCTCCGGG TGTAAGCATG 
CAGACGGTGC GGGTCACGGT GCTGGATGAC CTCGGACAGC CTGTTCTGGA AGGGACAGAA 
AGATTTGAGC TTGTTCTCCG GATGCCCTCC AATGGAATTC TTGGAGAGCC GGCCAAAGTC 
ACAGTCTTTA TCAACGACTC CATTTCAGAC CTGCCAAAGG TGCAGTTTAA ACAGGCTGTC 
GTGACTGTAG AGGAGAGTGA AGGTCATGTC ACTGTAATGG TGCACCGCAG TGGAGACATT 
AAACACAAGT CCAGTGTCCG CTGCTACACG CGGCAGGGCT CCGCACAGGT GGTATACGAC 
TTTGAGGAGA GACCAAACAC TGACGCCTCC ATCGTAACTT TCTTGCCTGG TGAAGTTGAT 
AAACCTTGTG TCTTGAAACT GGTGGATGAT TCAATTCATG AGGAGGAGGA AGAACTGAGG 
CTCGTTTTGG GAAATGCAAA GAGTGATTCG CCATACGGAG CGTCCATTGG AAACCAGAAT 
GAAACACTCA TAAAGATCAA GGACAATGCT GACAAATCCA TCATAAAGTT TGCCGAGACG 
AAGTTCAGTG TTAGCGAACC GAAAGAGTCT GGGCAGGTGA CATTAGTAAA GATCCCCATC 
CGCAGAACAG GAGACTCTTC CAAAGTGTCC CTAGTGAGGG TGCACACTAA AGACGGCTCT 
GCCATATCCG GAGAAGACTA TCATCCTATC TCCCTCGATG TGGAGTTTAA AGCAGAAGAG 
ATCGAGCATG TAGTTGAAGT GGAGGTTTTG TTTGATGGAA TAAGAGAGAT GAGAGAGGCC 
TTCACTGTCC ATTTAAAACC AGATGAGAAC ATGATTGCTG AAATACAGAT GACAAAAGCA 
ATAGTGTACA TCGAGGAGAC CAACAGCATG GCTGATGTCA CATTCCCTTC ATTGCCACAT 
ATTGTGTCAC TGCTGCACTA TGACAATATC AGAACAAGCA AACAAAGTGC TCATCCACCC 
GCTGGATATC CTGTCGTCTG TGTCACGGCC TGCAACCCCA AATATCCAGA CTACGACAAA 
ACAGGCTCCA TCTGCATTAG CGAACGCATC AACAACACGC TGACTCGTTA CCGATGGCTC 
ATTAGCGCCC CCAGCGGTCC TGATGGCGTC ACCAGCCCCA TGAGGGAGGC TGACTTCGAC 
ACCTTCTTCA CATCCTCCAA AATGATCACC CTAGACTCCA TTTACTTCCA AGCGGGCTCC 
AGAGTGCAGT GTGCAGCCCG TGCCGTGAAT TCAAATGGAG ACGAGGGTTT GGAACTGAGC 
AGCCCAATAG TGTCTATTAG CATGGAGGAA GGGATGTGCC AACCACGAAA ATTGGGCACA 
GTGGGTGCTG AGCCCTTTTC TGCCAAACTA CGTTATACGG GCGCTGAAGA CCTTGAATAT 
GCCAATCTCA TCAAGCTTAC TGTCACCATG CCCCACATAG ACGGCATGCT CCCCGTGGTC 
TCCACTCGTG CTCTGTCCAA TTTTGACCTG ACTCTGAGCC CCGATGGCAC TCGCGTGGGA 
AACCACAGGT GCTCCAATCT GCTGGACTTT AATGAGGTGT CCACACGGCA CGGCTTCCTG 
ACCAAAGCCA CCCGCAATAT CCAGAGCGTT GGAGAGACCC TACCTTATCA GTACAGCCCT 
GTCCTCCGAG GCCAGGGCAC TTTACGCTTC TACAGGAACC TGAACCTGGA GGCCTGTCTG 
TGGGAGTTTG TGAGTTATTA TGACATGTCA GAGCTGCTCA CAGACTGCGG GGGAACCATC 
GGGACAGATG GCCAGGTGCT GAACCTGGTG CAGTCATACG TGACCCTGCG TGTTCCTCTT 
CACGTCTCAT ACGTGTTTCA TTCCCCTGTG GGTGCTGGAG GCTGGCAGCA TTTCGACCTG 
CACTCCGAGC TCCGGCTCAC CTTTGTTTAT GACACTGCCA TCTTGTGGAA GGACGGAGTC 
GGCAGCCAAA CACAGGCTGA TCTACAAGGA GCTTTATATC CCACCAGCAT GAGGATTAAC 
GAGCAGGGGA GACTCGTGGT CAACTTCCGC ACAGAGGCCC GCTTCAGGGG TTTATTTGTA 
GTGGGGCATC CTGCAAATTT GAGGAGTCTG AAAACTACAG CGTCCACAGT GTCTACAGTG 
ATGAGTGCAG ATCACCCAGG CTTGACCTTC AACCTAACTC TGGTCAGGAC GGAGCCGACC 
TACAGCCAAC CCGTCCAGCA GTGGATGTTT ATCTCAGACT TCGCTGTTCG TGATTATTCT 
GGCACATACA CAGTGAGCTT GATCCCCTGT ACAGCTGCTC TGAATGTGGA GTTCACAGTT 
CCTCCAGTCT GCAACCCTAG AGAAGCCATC ACGTTTGATC TCGATATCCG CTTCCAGCAG 
GTGAGTGACC CTGTGGCTGT GGAGTTCAGT CTGAACACAC AGATGTTCCT GCTGTCTAAG 
AAAGTGCTGT GGCTGTCAGA TGGATCAATG GGCTTTGGTC AGGAAAGTGA CGCCGCTTTC 
TCTGAAGGTG ACGTGATCTA TGGACGTGTG ATGGTGGACC CGGTACAGAA TTTGGGCGAC 
TCTTTCTACT GTAACATTGA GAAGGTGTAT CTGTGCACTG GAGCTGATGG ATATGTTCCT 
AAATACAACC CCAGCAAGTC TGAGTTTGGA TGCCTTGCTG ACTCTCCGTC TCTGCTCTAC 
CAATTCAAGA TTATTGACAA AGCTCAGCCA GAAACTCAGG CCAGAAGTTT TGGGAATGTG 
GACTTCAATG CTCTGCTGGC TGTAGATGAC CCATCGGCCC TCTCTCTGGT CAAACAGCCT 
GGATCTGATG GCTTCAGACT CCATTCTACA CCCCTCTTCC AGGTGGCAGT AGGGAGAGAG 
TGGTTCATTC ACACCATCTA CACTGTGCGT TCAAAAGACA ACGCCAAACG TGGCATCGGC 
AAACGAAGTG TCGAACAGCA CTTGATAACA TCAGGAGACC TTAAAGAGTC AAGAAGACGC 
TCCAGACGCT CCAACAGAGA CGTTCCCACA CTGGCTGAGC AGATTGGCCC CGAAAACAAT 
CGAGGAACCA ACATCATGCA CATCGCTCTA GACCGCAGCC GCCGCACTGC ATCTGAAGAA 
GAGCTGTTTG CGGAGGGACT TTCTCCACGA GGACTCAGTG GACGACAGGC AGAAGAAGAA 
GCGGTGACGG TGGCAGGAGC TCTTGTGGGT TTGCTGCTCA CCGTTCTTGT GTTCGTAGCA 
TTAGCATTGG TGGTTCGCTC AAGGCGGACG CCAGCTAAAA AGGCCCCAAG AGTATCACCC 
AGCCTGCAGC CGGTCATTGT GCGGAGCAGT CTTGATAATG GGGACAGTTC AGAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages.
Development (Cambridge, England)142(2)320-30(2015 Jan)
Nagendran M,Arora P,Gori P,Mulay A,Ray S,Jacob T,Sonawane M

AMACO is a component of the basement membrane-associated Fraser complex.
The Journal of investigative dermatology134(5)1313-1322(2014 May)
Richardson RJ,Gebauer JM,Zhang JL,Kobbe B,Keene DR,Karlsen KR,Richetti S,Wohl AP,Sengle G,Neiss WF,Paulsson M,Hammerschmidt M,Wagener R

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frem2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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