FREM2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM2
Entrez Gene ID	486002
Full Name	FRAS1 related extracellular matrix protein 2
General protein information	Preferred Names FRAS1 related extracellular matrix protein 2 Names FRAS1-related extracellular matrix protein 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_543127.5	XP_543127.3	FRAS1-related extracellular matrix protein 2
XM_543127.6	XP_543127.3	FRAS1-related extracellular matrix protein 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	FREM2	NP_997244.3
Danio rerio (zebrafish)	frem2b	NP_001131133.1
Pan troglodytes (chimpanzee)	FREM2	XP_522664.2
	FREM2	XP_001086486.1
Bos taurus (cattle)	FREM2	XP_002691845.1
Canis lupus familiaris (dog)	FREM2	XP_005635476.1
Rattus norvegicus (Norway rat)	Frem2	NP_001232907.1
Mus musculus (house mouse)	Frem2	NP_766450.2
Gallus gallus (chicken)	FREM2	XP_417087.3
Danio rerio (zebrafish)	frem2a	NP_001131129.1
Xenopus tropicalis (tropical clawed frog)	LOC100496696	XP_004912033.1

Gene Ontology

The following FREM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe25340	XM_543127.6 Latest version!	Canis lupus familiaris FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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OCe25340	XM_543127.5	Canis lupus familiaris FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM2 ( XM_543127.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_543127.3 Canis lupus familiaris FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

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CloneID	OCe25340
Clone ID Related Accession (Same CDS sequence)	XM_543127.5 , XM_543127.6
Accession Version	XM_543127.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	FRAS1-related extracellular matrix protein 2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_543127.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCGG CCGGGGCCCC CGGGATCGCG TCCCGCGCCG CCACCGGTGC TCAGCCGGTG 
CCCCGGCTGC CGCCTCCCCT GCTGTTGCTA CTGCCGCTTC TCCTGTCCCC GGTAAGCCGC 
GCCCGGGCAC AGCCCGCTGC CCCGGGCCGG GAGCTGCTGT CCCCGGATCC CGCGGGCGCT 
GCGGGGGTCC TCGCCGAGGA GGCCGTGGTG CTGGCGAACC GGGGGCTCCG GGTGCCCTTC 
GGCCGCGAGG TCTGGCTGGA TCCGCGGCGC GACCTGGTGC TGCAGGTGCA GCCGGGGGAC 
CGCTGCGCGG TGACCGTGCT GGACAGTGAC GCGCTGGCGC AGAGGCCGGG CCGCCTGAGC 
CCCAAGCGCT TCCCGTGCGA CTTCGGCCCC GGGGAGGTGC GCTACTCGCA CCTGGGCGCT 
CGCAGCCCGT CGCGGGACCG CGTGCGGCTG CAGCTGCGCT ACGACGCGCC GGGAGGGGCC 
GCAGTGCTAC CGTTGGCGCT GGAGGTGGAG GTGGTCTTCA CCCAGCTGGA GGTCGTGACT 
CGGAACCTGC CCCTCGTCGT GGAGGAGCTG CTGGGGACCA GCAACGCCCT GGACGCGCGG 
AGCCTGGAGT TCGCCTACCA GCCCGAGACC GAGGAGTGTC GCGTGGGCAT CCTGTCGGGC 
TTGAGCGCGC TGCCCCGCTA CGGGGAACTG CTGCGCTACC CGCAGGTTCC CGGTGGAGCC 
GGCGACGGGG GCGCCCAGGA GCCTCTCCTG ATGGACTGCA AGGCCTTCCA GGAGCTGGGC 
GTGCGCTACC GCCACACGGC CCCCGGCCGG TCGCCCAACA GGGACTGGGT GCCCATGGTG 
GTGGAGCTGC GCGCTCGAGG CTCGCCAGTG GGCAGGCCTG CTTTGAAACG GGAGCACTTC 
CAGGTGCTGG TGAGAATCCG AGGGGGGGCG GAGAACACTG CGCCCAAGCC CAGTTTTGTG 
GCCATGATGA TGGTGGAGGT GGATCAGTTT GTCCTGACCG CCCTGACCCC CGACATGCTG 
GCGGCGGAGG ATGCAGAGTC GCCCCCTGAC CTGCTGGTCT TCAACCTCAC CGCTCCCTTC 
CAGCTGGGCC AGGGCTACCT GGTAAGTACC GATGACCGCA GCCTGCCCCT CTCCTCCTTC 
ACTCAGAGGG ATCTGCGGCT CCTGAAGATC GCCTACCAGC CGCCCTCTGA GGACTCTGAC 
CGAGAGCGGC TCTTTGAACT GGAGTTGGAG GTAGTGGATC CAGAAGGAGC AGCCTCAGAC 
CCTTTTGCTT TCATGGTTGT GGTGAAGCCC ATGAACACGC TGGCTCCAGT GGTCACCCGG 
AACACAGGCC TCCTTCTCTA CGAGGGCCAG TCCCGGCCCC TCACAGGCCC CGCAGGCAGT 
GGACCACAAA ACTTGGTCAT CAGCGATGAG GATGACCTGG AGGCAGTGCA GCTGGAGGTG 
GTGGCTGGGC TCCGGCATGG TCACCTTGTC CTCCTGGGTG CCACCCACGG CAACTCGGCC 
CCTAAGACCT TTTCGGTGGC CGAGCTAGCG GCCGGCCAGG TGGTCTACCA TCATGATGAC 
AAAGATGGCT CACTGAGTGA CAACCTTGTA CTTCGAATGG TGGACGGAGG ACGCAGGCAC 
CAGGTGCAGT TCCTGTTTCC CATCACCTTA GTGCCTGTGG ATGACCAGCC ACCAGTCCTC 
AATGCTAACA CGGGGCTGAC ACTGGCGGAA GGTGAAACGG TGCCCATCCT GCCCCTTGCT 
CTAAGTGCAG CTGACATTGA CTCAGAGGAC TCTCTGCTAC TTTTTGTGCT GGAATCGCCC 
TTCTCAGCGA TAGGGCATCT GCTTCTCCGC CAAACTCATC CTCCGCACGA GGAGGAGGAG 
TTTACTGGGA GCCCTTGGAG GAAACAGGGA GAGTTCTATG AGAAAGCAGT GACAGAATGG 
CGGCAGCAGG ACATAAGAGA GGGGAGGCTG TTCTACAGGC ACTCGGGGCC CCATAGTCCT 
GGGCCAGTGA TTGACCAGTT CACGTTTCGC GTCCAAGATA ACCATGACCC CCCTAATCAG 
TCAGGACCGC ACAGGTTTGT GGTGCGCATT CATCCGGTGG ATCGCCTCCC TCCAGAGTTG 
GGCAGTGGCT GTCCCCTGCG GATGGTGGTG CAGGAGTCCC AGCTCACCCC GCTGAGGAAG 
AAGTGGCTGC GCTACACCGA CCTGGACACA GATGACCGGG AACTCCATTA CACGGTGACC 
CAGCCCCCCA TGGACACAGA TGAAAACCAC TCGCCAGCCC CCCTGGGCTC CCTGGTCCTA 
ACAGACCAGC CCTCGGTCGT GGTGGCCCAT TTCACCCAAG CCCAGATCAA CCACCATAAA 
ATTGCTTACA AACCTCCAGA TCAAGAACTA GGAGTGGCTG CCCGAGTGGC CCAGTTCCAG 
TTCCAGGTGG AAGACCAAGC TGGGAATGTG GCCCCAGGCA CCTTCACCCT TTACCTGCAG 
CCAGTGGACA ACCAACCACC TGAGATCCTC AACACTGGTT TCACTGTTCA GGAGAAGGGC 
CACCATGTCC TGAGCGAGAC CGAGTTGCGT GTGGATGACG TAGACACAGA CGTGGCACAC 
ATCTCTTTCA CTCTCACTCA GGCACCCAAA CACGGCCACG TGAGAGTGTC TGGACAGATT 
TTGCACGTAG GGGAGGTCTT CCACTTGGAA GATATAAAAC AGGGTCGGAT TTCCTATGCA 
CATAATGGAG ATGAGTCCCT CACTGACAGC TGCTCCTTGG AGGTCAGCGA CACGCACCAC 
GTGGTGCCAA TCACTCTCAG AGTGAACATC CAGCCAGTGG ATGATGAGGT GCCCATGCTG 
AGCCTTTCTG CTGGCACTCT GGGGTCATAT CTAGATGTTT TAGAAAATGG AGCTACCGAA 
ATCACTGCCA GTGTTATCAA GGGGACTGAT GAGGACACTG ATGACTTGAT GTTGACTTTC 
CTCTTGGAAG ATCCACCCTT ATATGGTGAA ATCCTGGTCA ACGGAGTTCC AGCAGAGCAG 
TTTACCCAAA GAGACATTTT AGAGGGCTCT GTCGTCTATG CCCACACTAG TGGTGAGATA 
GGCCTGTTGC CCAAAGGGGA CTCTTTTAAC CTGAGCCTGT CGGATATGTC TCAAGAATGG 
GTAATAGGTG GCAGTACTAT CCAAGGAGTC ACCGTGTGGG TGACCGTCCT CCCTGTCGAT 
AGCCAGGCTC CAGAAATTTC TATAGGTGAA CAGTTTATAG TTGTGGAAGG TGATAAGAGT 
GTTGTAACAT CAGCACATAT AAGTGCTGAA GATACCGATT CACTAAATGA TGACATCTTA 
TGCACTATAG TTATTCAGCC TACGTCCGGT TATGTTGAAA ACATTTCTCC AGCCCCAGGC 
TCTGAGAAGT CAAGAGCAGG AGTTGCCATA AGTGCCTTTA CCGTGAAAGA CCTCAGGCAG 
AGTCATATTA ACTATGTCCA GAGTGTCCAT AAAGGGGTAG AACCTGTGGA AGATCGATTT 
ATATTTCGTT GCTCTGATGG CATTAACTTT TCAGAGAGAC ATTTATTCCC CATTGTCATC 
ATTCCCACCA ATGATGAACA GCCAGAAATA TTTATGAGAG AATTTATGGT GATGGAAGGC 
ATGAGTCTGG TGATCGACAC GCCCATCCTC AATGCTGCTG ATGCTGACAT TCCCCCCGAT 
GACTTAACTT TTACTGTTAC CCAATTTCCT ACTCGCGGTC ACATTGTGAA CCAGCTGATA 
AATGGCACAG TTTTGGTTGA AAGCTTTACC TTGGACCAGA TCATAGAGAG TTCTAGCATT 
ATTTATGAGC ATGATGACTC TGAGACCCAG GAAGACCGTT TTGTGATTAG ACTAACAGAT 
GGTAGGCATT CAGTGGAAAG GACGGTCCCC ATTATTGTTA TCCCAGTTGA TGATGAGACC 
CCCAGAATGA CCATCAATAA TGGACTAGAA ATAGAAATTG GGGAAACCAA AATTATCAAC 
AACAAAATAT TAATGGCAAC TGATTTAGAT TCGGAAGACA AATCTTTGGT TTATGTTATT 
CGTTATGGGC CAGGACATGG CTTATTGCAG AGGCAAAAAC CTACAGGTGC CTTTGAAAAT 
ATCACACTCG GCATGAATTT TACCCAGGAT GAAGTGGACA GAAACTTAAT TCAGTATGTC 
CATGTTGGAC AAGAGGGCAT TCGGGACCTT ATTAAATTTG ATGTGACTGA CGGAATAAAC 
GCCCTGATAG ATCGCTACTT TTACATTTCC GTCGGGAGTA TTGACACTGT CTTCCCAGAT 
GTGATAAGCA AGGGGGTATC CTTGAAAGAA GGAGGCAAAG TCACCCTCAC AACAGACCTC 
CTAAGCACAA GTGACCTGAA CAGTCCTGAT GAAAACTTAG TTTTCACCAT CACCAGGGCT 
CCCACGAGAG GTCACTTAGA GTGCACAGAT CAACGTGGAG CTTCCATCAT GTCTTTCACT 
CAGCTCCAAC TGGCTGGCAA CAAAATCTAC TACATCCACA CAGCTGATGA TGAGGTCAAG 
ATGGACAGTT TTGAGTTTCA AGTCACGGAT GGACGTAACC CTGTCTTCCG GACATTCCGT 
ATCTCCATTA GTGATGTGGA TAACAAAAAG CCAGTAGTCA CCATCCATAA CCTGGTTGTC 
AGCGAGGGTG AAAACAAGCT GATCACTCCC TTTGAACTCA CTGCCGAAGA CCCAGATACT 
CCTGACAAAC TCCTGAAGTT CATCGTCACC CAGGTGCCTG TTCATGGTCA ACTCTTATTC 
AACAATACCA GACCTGCCAT GGTTTTTACC AAGCAAGACT TGAATGAAAA CTTAATCAGC 
TACAGACATG ATGGCACCGA GTCAAGCGAA GACAGCTTCT CCTTCACGGT GACTGACAGT 
TCTCATACAG AGTTCTATGT CTTCCCTGAT ACAATATTTG AAACGAGGAG ACCCCAAGTG 
ATGAAAATCC AGGTCTTAGC TGTTGACGAT AGCATCCCCC AGATTGCAGT GAACAAAGGG 
GCTTCCACGC TTCGCCCTCT GGCTACCGGC CACTTGGGGT TCATGATCAC AAGCAAAGTA 
TTGAAAGCAG AGGACAGAGA CAGCTTACGT TTTTCTCTCA GGTTTATTGT GACAGGAGCT 
CCTCAACATG GGTATCTCCT CAATCTGGAC AGAGGCAACC ACAGTGTCAC TCAGTTTACA 
CAAGGTGACA TTGATGACAT GAAAATATGC TACATTTTAA AAGAGGGGGC CAATGCCACA 
AGTGATGTGT TCCATTTTAC AGTTGAAGAT GGTGGAGGAA ACAAATTAAC CAAGCAACAT 
TTTCGTCTGA ATTGGGCATG GATCTCCTTT GAAAAGGAAT ATTACCTGAT CAATGAGGAC 
TCCAAATTTC TAGATGTCGT TCTTAAACGT AGAGGGTTTT TGGGAGAGAC TTCATTTATA 
AGTATTGGCA CAAGAGATGG GACTGCAGAA AAAGACAAAG ACTTCAAGGG CAAAGCACAG 
AAACAAGTAC AGTTCAACCC AGGCCAGACC AGGGCAACCT GGAAAGTGCG GATCATGAGC 
GATGGGGAGC ATGAGCAGTC GGAGACCTTC CAGGTGGTTC TCTCAGAGCC GGTGCTGGCT 
GCTTTGGAGT TTCCCACAGT GACCACAGTG GAGATCATTG ACCCAGGAGA TGAGTCAACT 
GTATTTATTC CCCAGTCTGA ATACTCTGTT GAAGAAGACG TCGGTGAGCT GTTCATTCCC 
ATTAGGAGGA GTGGAGACGT GAGCCAGGAG CTGATGGTGA TCTGTTACAC ACAACAAGGA 
ACAGCAGGCG GAACTGTACC AACATCAGTG CTGTCCTATT CAGACTACAT ATCCAGGCCG 
GAGGACCACA CCAGTGTCAT CCGCTTTGAC AAAGATGAAC ACGAGAAAAT GTGTCGCATC 
GTTGTTATTG ATGACTCCCT GTATGAAGAG GAGGAAACAT TCCATGTCCT TCTGAGCATG 
CCCATGGGAG GGAGGATTGG ATCAGAGTTC CCAGGGACTC AGGTTACAAT CATCCCTGAC 
AAAGATGATG AACCTGTCTT CTACTTTGGT GATGTCGAGT ACCTGGTGGA CGAGAGCGCC 
GGCTATGTGG AATTGCGGGT GTGGAGAACA GGCACCGACC TGTCCAGGTC TTCCAGTGTC 
ACCGTGAGGT CTCGGAAAAC AGATCCTCCC TCTGCTGATG CCGGAACAGA TTATGTGGGA 
ATCAGTCGGA ATTTAGATTT TGCACCTGGA GTCAACATGC AGACTGTTCG TGTTATCATC 
CTGGATGATC TTGGGCAACC AGTGCTGGAG GGAATTGAGA AATTTGAACT GGTGCTTCGT 
ATGCCTATGA ACGCTGCCCT CGGCGAGCCC AGCAAAGCCA CGGTTTCCAT AAATGATTCT 
GTCTCCGACT TGCCTAAGAT GCAGTTCAAA GAACGAGTAT ATACTGGCAA TGAAAACGAT 
GGGCAGATAG TTGCAATGAT CCACAGGAGT GGAGACATTC ACTATAGGTC TTCAGTGAGA 
TGCTACACAC GACAGGGGTC TGCACAGGTG ATGACGGACT TTGAGGAACG TCCAAACATT 
GATAGCTCTG TCATCCTGTT CCTCCCTGGT GAGACTGAAA AGCCTTGTGT ACTTGAGCTG 
ATGGATGACA AACTCTATGA AGAGGTAGAG GAACTCCGCC TGGTTCTTGG AACCCCAAGT 
AGCGACTCCC CCTTTGGGGC TGCAGTTGGG GAACAAAATG AAACTCTGAT AAGGATCCAA 
GATGATGCTG ATAAGACTGT TATTAAATTT GGAGAAACCA GATTTAGCGT GAGTGAACCC 
AAGGAACCAG GACAGTCGGT GGTTGTAAAA ATTCCAGTGA TTCGCCAAGG AGATACTTCA 
AAGGTTTCAA TTGTGAGAGT CCACACCAAG GATGGCTCAG CCACTTCTGG AGAAGACTAT 
CACCCTGTGT CAGAAGAAAT TGAATTTAAG GAGGGAGAAA CCCAGCATAT TGTTAACATT 
GAAGTAATCT TCGATGGGAT CAGAGAGATG AGAGAGGCCT TCACTGTCCA CCTAAAACCC 
GATGAGAACA TGGTGGCAGA GACGCAGCCC ATTGTGTATA TAGAAGAAAT GAACAGCATG 
GCGGAAGTCA CTTTCCCATC TGTCCCTCAA ATTGTGTCAT TACTGATGTA TGATGACACT 
TCCAGAGCTA AAGATAGTGC AGGACCTGTG TCTGGCTATC CTGTTGTCTG TATTACAGCT 
TGCAACCCTA AATACTCAGA CTATGACAAA ACAGGTTCTA TTTGTGCAAG TGAAAATATC 
AATGACACCT TGACCCGGTA CCGATGGCTA ATTAGTGCAC CCGCTGGCCC TGATGGTGTG 
ACCAGCCCCA TGAGAGAAGT GGACTTTGAC ACCTTTTTTA CATCGTCCAA GATGATCACC 
TTGGATTCCA TATACTTCCA GCCCGGCTCC AGAGTGCAGT GTGCGGCCCG TGCTGTGAAT 
GCCAATGGCA ATGAGGGCCT GGAGCTGATG AGCCCTATTG TAACCATCGG CAGAGAAGAA 
GGTCTTTGTC AGCCCAGGGT GCCAGGGACA GTGGGAGCAG AGCCATTCTC AGCTAAGCTG 
CGCTACACAG GCCCCGAGGA CCCAGAGTAC ACGAACTTTA TCAAGCTCAC CGTCACGATG 
CCACACATAG ATGGTATGCT CCCTGTGATC TCCACTAAAG AGCTTTCCAA CTTTGAGCTC 
ACCCTCAGTC CTGATGGCTC AAGAGTTGGA AACCACCAGT GCTCCAATCT TCTAGATTAC 
ACTGAAGTGA AGACTCACCA TGGCTTCTTA ACTGATGCTA CGAAAAATCC AGAAGTAATT 
GGAGAGACTC ATCCTTACCA GTACAGCTCA TCTATCAGAG GTTCCAGTAC CTTACGCTTC 
TATCGAAACT TGAACCTGGA AGCCTGTTTA TGGGAGTTTG TTAGCTACTA TGACATGTCA 
GAACTTCTCA CCAACTGTGG AGGAACCATC GGGACAGACG GACAGGTCCT GAATCTGGTG 
CAGTCATATG TGACCCTTCG GGTGCCTCTG TATGTTTCGT ATGTGTTCCA TTCCCCCGTT 
GGCGTAGGGG GCTGGCAGCA TTTTGACTTG AAGTCGGAGC TCCGCCTGAC TTTCGTGTAT 
GACACTGCCA TCTTGTGGAA TGAGGGCATC GGCAGCCCAC CAGAAGCCGA GCTCCAAGGT 
TCTCTCTACC CGACTAGCAT GCGCATTGGT GAAGAGGGGC GCTTGGTTGT GAACTTCAAG 
ACAGAGGCTC AGTTCCATGG ATTGTTTGTG CTGTCTCATC CCGCATCCTT TACAAGCTCA 
ATGATCATGT CAGCTGATCA CCCGGGCCTG ACCTTCTCCT TGCGCCTCAT CAGGAGTGAA 
CCAACCTATA ACCAGCCAGT GCAGCAGTGG AGCTTTGTGT CTGACTTTGC AGTACGTGAC 
TATTCGGGGA CCTACACCGT GAAACTGCTA CCTTGCACTG CTCCATCGCA TCAGGAGTAC 
CACCTGCCAG TCACCTGCAG CCCCAGAGAG CCCATCACCT TTGACCTCGA CATACGATTC 
CAGCAGGTCA GTGATCCAGT GGCGGCTGAG TTTAGCTTGA ACACCCAAAT GTACCTCCTG 
TCCAAGAAGA GTCTCTGGTT GTCTGACGGA TCCATGGGAT TTGGGCAAGA GAGTGATGTT 
GCCTTCACAG AAGGTGATAT AATCTATGGT CGTGTCATGG TAGATCCTGT CCAGAATCTG 
GGTGACTCCT TTTACTGCAG CATTGAGAAG GTGTTCTTGT GCACTGGAGC TGATGGCTAT 
GTTCCCAAAT ATAATCCCAC AAATGCAGAA TATGGCTGCT TAGCCGACTC TCCTTCACTC 
TTATATAGAT TTAAAATTGT GGACAAAGCT CAACCAGAGA CACAAGCCAT CAGTTTTGGA 
AATGTCCTGT TTAATGCCAA ACTAGCAATG GATGATCCTG AAGCTATTCT CTTAGTGAAT 
CAGCCTGGAT CTGATGGATT TAAAGTCGAC TCCATGCCAC TCTTTCAGGT CGCTCTGGGC 
CGAGAATGGT ACATACACAC GGTCTACACA GTAAGATCAA AAGACAACGC CAATCGAGGC 
ATTGGCAAAA GAAGCACTGA GCACCAGCAC CACTCCTTAG TGAGACCAGG GAAGCCCCAG 
GCAACTAGCA AGGGCCGGAA GAAGAGGGAG ATCAGGAGCC CACCCCCACT GGCCTGGCAA 
ATAGGTGCTG AGAACAACCG GGGAACAAAC ATCCAGCACA TTGCCCTGGA CCGCAGCAGC 
CGGAAGCTGA TCCCCCAAGG CCGAGTTCCT CCTGACGGCG TCCTCCCCCG GGAGCTCAAC 
CGTCCCAGCT CTGAGGTCAG CCTGGTCACA GTCGTTGGCG GCATCGCGGT GGGATTGCTA 
ACCCTCTGCC TTGCTGCCAT CACAGTGATG ATGTGCAAGA GCAAGAAGGG CTCCAGGAGA 
AAGGATGCTC TAAAGTGCTC AGGCAGCAGC AAGCCCATGA TGCCTCCGCA GAGCCACCAC 
TGTGACAGCT CAGAGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_543127.3
CDS	186..9686
Translation	MRAAGAPGIASRAATGAQPVPRLPPPLLLLLPLLLSPVSRARAQPAAPGRELLSPDPAGAAGVLAEEAVVLANRGLRVPFGREVWLDPRRDLVLQVQPGDRCAVTVLDSDALAQRPGRLSPKRFPCDFGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAAVLPLALEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAYQPETEECRVGILSGLSALPRYGELLRYPQVPGGAGDGGAQEPLLMDCKAFQELGVRYRHTAPGRSPNRDWVPMVVELRARGSPVGRPALKREHFQVLVRIRGGAENTAPKPSFVAMMMVEVDQFVLTALTPDMLAAEDAESPPDLLVFNLTAPFQLGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDRERLFELELEVVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLLLYEGQSRPLTGPAGSGPQNLVISDEDDLEAVQLEVVAGLRHGHLVLLGATHGNSAPKTFSVAELAAGQVVYHHDDKDGSLSDNLVLRMVDGGRRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETVPILPLALSAADIDSEDSLLLFVLESPFSAIGHLLLRQTHPPHEEEEFTGSPWRKQGEFYEKAVTEWRQQDIREGRLFYRHSGPHSPGPVIDQFTFRVQDNHDPPNQSGPHRFVVRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELHYTVTQPPMDTDENHSPAPLGSLVLTDQPSVVVAHFTQAQINHHKIAYKPPDQELGVAARVAQFQFQVEDQAGNVAPGTFTLYLQPVDNQPPEILNTGFTVQEKGHHVLSETELRVDDVDTDVAHISFTLTQAPKHGHVRVSGQILHVGEVFHLEDIKQGRISYAHNGDESLTDSCSLEVSDTHHVVPITLRVNIQPVDDEVPMLSLSAGTLGSYLDVLENGATEITASVIKGTDEDTDDLMLTFLLEDPPLYGEILVNGVPAEQFTQRDILEGSVVYAHTSGEIGLLPKGDSFNLSLSDMSQEWVIGGSTIQGVTVWVTVLPVDSQAPEISIGEQFIVVEGDKSVVTSAHISAEDTDSLNDDILCTIVIQPTSGYVENISPAPGSEKSRAGVAISAFTVKDLRQSHINYVQSVHKGVEPVEDRFIFRCSDGINFSERHLFPIVIIPTNDEQPEIFMREFMVMEGMSLVIDTPILNAADADIPPDDLTFTVTQFPTRGHIVNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDRFVIRLTDGRHSVERTVPIIVIPVDDETPRMTINNGLEIEIGETKIINNKILMATDLDSEDKSLVYVIRYGPGHGLLQRQKPTGAFENITLGMNFTQDEVDRNLIQYVHVGQEGIRDLIKFDVTDGINALIDRYFYISVGSIDTVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPTRGHLECTDQRGASIMSFTQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSEGENKLITPFELTAEDPDTPDKLLKFIVTQVPVHGQLLFNNTRPAMVFTKQDLNENLISYRHDGTESSEDSFSFTVTDSSHTEFYVFPDTIFETRRPQVMKIQVLAVDDSIPQIAVNKGASTLRPLATGHLGFMITSKVLKAEDRDSLRFSLRFIVTGAPQHGYLLNLDRGNHSVTQFTQGDIDDMKICYILKEGANATSDVFHFTVEDGGGNKLTKQHFRLNWAWISFEKEYYLINEDSKFLDVVLKRRGFLGETSFISIGTRDGTAEKDKDFKGKAQKQVQFNPGQTRATWKVRIMSDGEHEQSETFQVVLSEPVLAALEFPTVTTVEIIDPGDESTVFIPQSEYSVEEDVGELFIPIRRSGDVSQELMVICYTQQGTAGGTVPTSVLSYSDYISRPEDHTSVIRFDKDEHEKMCRIVVIDDSLYEEEETFHVLLSMPMGGRIGSEFPGTQVTIIPDKDDEPVFYFGDVEYLVDESAGYVELRVWRTGTDLSRSSSVTVRSRKTDPPSADAGTDYVGISRNLDFAPGVNMQTVRVIILDDLGQPVLEGIEKFELVLRMPMNAALGEPSKATVSINDSVSDLPKMQFKERVYTGNENDGQIVAMIHRSGDIHYRSSVRCYTRQGSAQVMTDFEERPNIDSSVILFLPGETEKPCVLELMDDKLYEEVEELRLVLGTPSSDSPFGAAVGEQNETLIRIQDDADKTVIKFGETRFSVSEPKEPGQSVVVKIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHIVNIEVIFDGIREMREAFTVHLKPDENMVAETQPIVYIEEMNSMAEVTFPSVPQIVSLLMYDDTSRAKDSAGPVSGYPVVCITACNPKYSDYDKTGSICASENINDTLTRYRWLISAPAGPDGVTSPMREVDFDTFFTSSKMITLDSIYFQPGSRVQCAARAVNANGNEGLELMSPIVTIGREEGLCQPRVPGTVGAEPFSAKLRYTGPEDPEYTNFIKLTVTMPHIDGMLPVISTKELSNFELTLSPDGSRVGNHQCSNLLDYTEVKTHHGFLTDATKNPEVIGETHPYQYSSSIRGSSTLRFYRNLNLEACLWEFVSYYDMSELLTNCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWNEGIGSPPEAELQGSLYPTSMRIGEEGRLVVNFKTEAQFHGLFVLSHPASFTSSMIMSADHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLLPCTAPSHQEYHLPVTCSPREPITFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFTEGDIIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYNPTNAEYGCLADSPSLLYRFKIVDKAQPETQAISFGNVLFNAKLAMDDPEAILLVNQPGSDGFKVDSMPLFQVALGREWYIHTVYTVRSKDNANRGIGKRSTEHQHHSLVRPGKPQATSKGRKKREIRSPPPLAWQIGAENNRGTNIQHIALDRSSRKLIPQGRVPPDGVLPRELNRPSSEVSLVTVVGGIAVGLLTLCLAAITVMMCKSKKGSRRKDALKCSGSSKPMMPPQSHHCDSSEV

Target ORF information:

RefSeq Version	XM_543127.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_543127.5

ORF Insert Sequence:

ATGCGCGCGG CCGGGGCCCC CGGGATCGCG TCCCGCGCCG CCACCGGTGC TCAGCCGGTG 
CCCCGGCTGC CGCCTCCCCT GCTGTTGCTA CTGCCGCTTC TCCTGTCCCC GGTAAGCCGC 
GCCCGGGCAC AGCCCGCTGC CCCGGGCCGG GAGCTGCTGT CCCCGGATCC CGCGGGCGCT 
GCGGGGGTCC TCGCCGAGGA GGCCGTGGTG CTGGCGAACC GGGGGCTCCG GGTGCCCTTC 
GGCCGCGAGG TCTGGCTGGA TCCGCGGCGC GACCTGGTGC TGCAGGTGCA GCCGGGGGAC 
CGCTGCGCGG TGACCGTGCT GGACAGTGAC GCGCTGGCGC AGAGGCCGGG CCGCCTGAGC 
CCCAAGCGCT TCCCGTGCGA CTTCGGCCCC GGGGAGGTGC GCTACTCGCA CCTGGGCGCT 
CGCAGCCCGT CGCGGGACCG CGTGCGGCTG CAGCTGCGCT ACGACGCGCC GGGAGGGGCC 
GCAGTGCTAC CGTTGGCGCT GGAGGTGGAG GTGGTCTTCA CCCAGCTGGA GGTCGTGACT 
CGGAACCTGC CCCTCGTCGT GGAGGAGCTG CTGGGGACCA GCAACGCCCT GGACGCGCGG 
AGCCTGGAGT TCGCCTACCA GCCCGAGACC GAGGAGTGTC GCGTGGGCAT CCTGTCGGGC 
TTGAGCGCGC TGCCCCGCTA CGGGGAACTG CTGCGCTACC CGCAGGTTCC CGGTGGAGCC 
GGCGACGGGG GCGCCCAGGA GCCTCTCCTG ATGGACTGCA AGGCCTTCCA GGAGCTGGGC 
GTGCGCTACC GCCACACGGC CCCCGGCCGG TCGCCCAACA GGGACTGGGT GCCCATGGTG 
GTGGAGCTGC GCGCTCGAGG CTCGCCAGTG GGCAGGCCTG CTTTGAAACG GGAGCACTTC 
CAGGTGCTGG TGAGAATCCG AGGGGGGGCG GAGAACACTG CGCCCAAGCC CAGTTTTGTG 
GCCATGATGA TGGTGGAGGT GGATCAGTTT GTCCTGACCG CCCTGACCCC CGACATGCTG 
GCGGCGGAGG ATGCAGAGTC GCCCCCTGAC CTGCTGGTCT TCAACCTCAC CGCTCCCTTC 
CAGCTGGGCC AGGGCTACCT GGTAAGTACC GATGACCGCA GCCTGCCCCT CTCCTCCTTC 
ACTCAGAGGG ATCTGCGGCT CCTGAAGATC GCCTACCAGC CGCCCTCTGA GGACTCTGAC 
CGAGAGCGGC TCTTTGAACT GGAGTTGGAG GTAGTGGATC CAGAAGGAGC AGCCTCAGAC 
CCTTTTGCTT TCATGGTTGT GGTGAAGCCC ATGAACACGC TGGCTCCAGT GGTCACCCGG 
AACACAGGCC TCCTTCTCTA CGAGGGCCAG TCCCGGCCCC TCACAGGCCC CGCAGGCAGT 
GGACCACAAA ACTTGGTCAT CAGCGATGAG GATGACCTGG AGGCAGTGCA GCTGGAGGTG 
GTGGCTGGGC TCCGGCATGG TCACCTTGTC CTCCTGGGTG CCACCCACGG CAACTCGGCC 
CCTAAGACCT TTTCGGTGGC CGAGCTAGCG GCCGGCCAGG TGGTCTACCA TCATGATGAC 
AAAGATGGCT CACTGAGTGA CAACCTTGTA CTTCGAATGG TGGACGGAGG ACGCAGGCAC 
CAGGTGCAGT TCCTGTTTCC CATCACCTTA GTGCCTGTGG ATGACCAGCC ACCAGTCCTC 
AATGCTAACA CGGGGCTGAC ACTGGCGGAA GGTGAAACGG TGCCCATCCT GCCCCTTGCT 
CTAAGTGCAG CTGACATTGA CTCAGAGGAC TCTCTGCTAC TTTTTGTGCT GGAATCGCCC 
TTCTCAGCGA TAGGGCATCT GCTTCTCCGC CAAACTCATC CTCCGCACGA GGAGGAGGAG 
TTTACTGGGA GCCCTTGGAG GAAACAGGGA GAGTTCTATG AGAAAGCAGT GACAGAATGG 
CGGCAGCAGG ACATAAGAGA GGGGAGGCTG TTCTACAGGC ACTCGGGGCC CCATAGTCCT 
GGGCCAGTGA TTGACCAGTT CACGTTTCGC GTCCAAGATA ACCATGACCC CCCTAATCAG 
TCAGGACCGC ACAGGTTTGT GGTGCGCATT CATCCGGTGG ATCGCCTCCC TCCAGAGTTG 
GGCAGTGGCT GTCCCCTGCG GATGGTGGTG CAGGAGTCCC AGCTCACCCC GCTGAGGAAG 
AAGTGGCTGC GCTACACCGA CCTGGACACA GATGACCGGG AACTCCATTA CACGGTGACC 
CAGCCCCCCA TGGACACAGA TGAAAACCAC TCGCCAGCCC CCCTGGGCTC CCTGGTCCTA 
ACAGACCAGC CCTCGGTCGT GGTGGCCCAT TTCACCCAAG CCCAGATCAA CCACCATAAA 
ATTGCTTACA AACCTCCAGA TCAAGAACTA GGAGTGGCTG CCCGAGTGGC CCAGTTCCAG 
TTCCAGGTGG AAGACCAAGC TGGGAATGTG GCCCCAGGCA CCTTCACCCT TTACCTGCAG 
CCAGTGGACA ACCAACCACC TGAGATCCTC AACACTGGTT TCACTGTTCA GGAGAAGGGC 
CACCATGTCC TGAGCGAGAC CGAGTTGCGT GTGGATGACG TAGACACAGA CGTGGCACAC 
ATCTCTTTCA CTCTCACTCA GGCACCCAAA CACGGCCACG TGAGAGTGTC TGGACAGATT 
TTGCACGTAG GGGAGGTCTT CCACTTGGAA GATATAAAAC AGGGTCGGAT TTCCTATGCA 
CATAATGGAG ATGAGTCCCT CACTGACAGC TGCTCCTTGG AGGTCAGCGA CACGCACCAC 
GTGGTGCCAA TCACTCTCAG AGTGAACATC CAGCCAGTGG ATGATGAGGT GCCCATGCTG 
AGCCTTTCTG CTGGCACTCT GGGGTCATAT CTAGATGTTT TAGAAAATGG AGCTACCGAA 
ATCACTGCCA GTGTTATCAA GGGGACTGAT GAGGACACTG ATGACTTGAT GTTGACTTTC 
CTCTTGGAAG ATCCACCCTT ATATGGTGAA ATCCTGGTCA ACGGAGTTCC AGCAGAGCAG 
TTTACCCAAA GAGACATTTT AGAGGGCTCT GTCGTCTATG CCCACACTAG TGGTGAGATA 
GGCCTGTTGC CCAAAGGGGA CTCTTTTAAC CTGAGCCTGT CGGATATGTC TCAAGAATGG 
GTAATAGGTG GCAGTACTAT CCAAGGAGTC ACCGTGTGGG TGACCGTCCT CCCTGTCGAT 
AGCCAGGCTC CAGAAATTTC TATAGGTGAA CAGTTTATAG TTGTGGAAGG TGATAAGAGT 
GTTGTAACAT CAGCACATAT AAGTGCTGAA GATACCGATT CACTAAATGA TGACATCTTA 
TGCACTATAG TTATTCAGCC TACGTCCGGT TATGTTGAAA ACATTTCTCC AGCCCCAGGC 
TCTGAGAAGT CAAGAGCAGG AGTTGCCATA AGTGCCTTTA CCGTGAAAGA CCTCAGGCAG 
AGTCATATTA ACTATGTCCA GAGTGTCCAT AAAGGGGTAG AACCTGTGGA AGATCGATTT 
ATATTTCGTT GCTCTGATGG CATTAACTTT TCAGAGAGAC ATTTATTCCC CATTGTCATC 
ATTCCCACCA ATGATGAACA GCCAGAAATA TTTATGAGAG AATTTATGGT GATGGAAGGC 
ATGAGTCTGG TGATCGACAC GCCCATCCTC AATGCTGCTG ATGCTGACAT TCCCCCCGAT 
GACTTAACTT TTACTGTTAC CCAATTTCCT ACTCGCGGTC ACATTGTGAA CCAGCTGATA 
AATGGCACAG TTTTGGTTGA AAGCTTTACC TTGGACCAGA TCATAGAGAG TTCTAGCATT 
ATTTATGAGC ATGATGACTC TGAGACCCAG GAAGACCGTT TTGTGATTAG ACTAACAGAT 
GGTAGGCATT CAGTGGAAAG GACGGTCCCC ATTATTGTTA TCCCAGTTGA TGATGAGACC 
CCCAGAATGA CCATCAATAA TGGACTAGAA ATAGAAATTG GGGAAACCAA AATTATCAAC 
AACAAAATAT TAATGGCAAC TGATTTAGAT TCGGAAGACA AATCTTTGGT TTATGTTATT 
CGTTATGGGC CAGGACATGG CTTATTGCAG AGGCAAAAAC CTACAGGTGC CTTTGAAAAT 
ATCACACTCG GCATGAATTT TACCCAGGAT GAAGTGGACA GAAACTTAAT TCAGTATGTC 
CATGTTGGAC AAGAGGGCAT TCGGGACCTT ATTAAATTTG ATGTGACTGA CGGAATAAAC 
GCCCTGATAG ATCGCTACTT TTACATTTCC GTCGGGAGTA TTGACACTGT CTTCCCAGAT 
GTGATAAGCA AGGGGGTATC CTTGAAAGAA GGAGGCAAAG TCACCCTCAC AACAGACCTC 
CTAAGCACAA GTGACCTGAA CAGTCCTGAT GAAAACTTAG TTTTCACCAT CACCAGGGCT 
CCCACGAGAG GTCACTTAGA GTGCACAGAT CAACGTGGAG CTTCCATCAT GTCTTTCACT 
CAGCTCCAAC TGGCTGGCAA CAAAATCTAC TACATCCACA CAGCTGATGA TGAGGTCAAG 
ATGGACAGTT TTGAGTTTCA AGTCACGGAT GGACGTAACC CTGTCTTCCG GACATTCCGT 
ATCTCCATTA GTGATGTGGA TAACAAAAAG CCAGTAGTCA CCATCCATAA CCTGGTTGTC 
AGCGAGGGTG AAAACAAGCT GATCACTCCC TTTGAACTCA CTGCCGAAGA CCCAGATACT 
CCTGACAAAC TCCTGAAGTT CATCGTCACC CAGGTGCCTG TTCATGGTCA ACTCTTATTC 
AACAATACCA GACCTGCCAT GGTTTTTACC AAGCAAGACT TGAATGAAAA CTTAATCAGC 
TACAGACATG ATGGCACCGA GTCAAGCGAA GACAGCTTCT CCTTCACGGT GACTGACAGT 
TCTCATACAG AGTTCTATGT CTTCCCTGAT ACAATATTTG AAACGAGGAG ACCCCAAGTG 
ATGAAAATCC AGGTCTTAGC TGTTGACGAT AGCATCCCCC AGATTGCAGT GAACAAAGGG 
GCTTCCACGC TTCGCCCTCT GGCTACCGGC CACTTGGGGT TCATGATCAC AAGCAAAGTA 
TTGAAAGCAG AGGACAGAGA CAGCTTACGT TTTTCTCTCA GGTTTATTGT GACAGGAGCT 
CCTCAACATG GGTATCTCCT CAATCTGGAC AGAGGCAACC ACAGTGTCAC TCAGTTTACA 
CAAGGTGACA TTGATGACAT GAAAATATGC TACATTTTAA AAGAGGGGGC CAATGCCACA 
AGTGATGTGT TCCATTTTAC AGTTGAAGAT GGTGGAGGAA ACAAATTAAC CAAGCAACAT 
TTTCGTCTGA ATTGGGCATG GATCTCCTTT GAAAAGGAAT ATTACCTGAT CAATGAGGAC 
TCCAAATTTC TAGATGTCGT TCTTAAACGT AGAGGGTTTT TGGGAGAGAC TTCATTTATA 
AGTATTGGCA CAAGAGATGG GACTGCAGAA AAAGACAAAG ACTTCAAGGG CAAAGCACAG 
AAACAAGTAC AGTTCAACCC AGGCCAGACC AGGGCAACCT GGAAAGTGCG GATCATGAGC 
GATGGGGAGC ATGAGCAGTC GGAGACCTTC CAGGTGGTTC TCTCAGAGCC GGTGCTGGCT 
GCTTTGGAGT TTCCCACAGT GACCACAGTG GAGATCATTG ACCCAGGAGA TGAGTCAACT 
GTATTTATTC CCCAGTCTGA ATACTCTGTT GAAGAAGACG TCGGTGAGCT GTTCATTCCC 
ATTAGGAGGA GTGGAGACGT GAGCCAGGAG CTGATGGTGA TCTGTTACAC ACAACAAGGA 
ACAGCAGGCG GAACTGTACC AACATCAGTG CTGTCCTATT CAGACTACAT ATCCAGGCCG 
GAGGACCACA CCAGTGTCAT CCGCTTTGAC AAAGATGAAC ACGAGAAAAT GTGTCGCATC 
GTTGTTATTG ATGACTCCCT GTATGAAGAG GAGGAAACAT TCCATGTCCT TCTGAGCATG 
CCCATGGGAG GGAGGATTGG ATCAGAGTTC CCAGGGACTC AGGTTACAAT CATCCCTGAC 
AAAGATGATG AACCTGTCTT CTACTTTGGT GATGTCGAGT ACCTGGTGGA CGAGAGCGCC 
GGCTATGTGG AATTGCGGGT GTGGAGAACA GGCACCGACC TGTCCAGGTC TTCCAGTGTC 
ACCGTGAGGT CTCGGAAAAC AGATCCTCCC TCTGCTGATG CCGGAACAGA TTATGTGGGA 
ATCAGTCGGA ATTTAGATTT TGCACCTGGA GTCAACATGC AGACTGTTCG TGTTATCATC 
CTGGATGATC TTGGGCAACC AGTGCTGGAG GGAATTGAGA AATTTGAACT GGTGCTTCGT 
ATGCCTATGA ACGCTGCCCT CGGCGAGCCC AGCAAAGCCA CGGTTTCCAT AAATGATTCT 
GTCTCCGACT TGCCTAAGAT GCAGTTCAAA GAACGAGTAT ATACTGGCAA TGAAAACGAT 
GGGCAGATAG TTGCAATGAT CCACAGGAGT GGAGACATTC ACTATAGGTC TTCAGTGAGA 
TGCTACACAC GACAGGGGTC TGCACAGGTG ATGACGGACT TTGAGGAACG TCCAAACATT 
GATAGCTCTG TCATCCTGTT CCTCCCTGGT GAGACTGAAA AGCCTTGTGT ACTTGAGCTG 
ATGGATGACA AACTCTATGA AGAGGTAGAG GAACTCCGCC TGGTTCTTGG AACCCCAAGT 
AGCGACTCCC CCTTTGGGGC TGCAGTTGGG GAACAAAATG AAACTCTGAT AAGGATCCAA 
GATGATGCTG ATAAGACTGT TATTAAATTT GGAGAAACCA GATTTAGCGT GAGTGAACCC 
AAGGAACCAG GACAGTCGGT GGTTGTAAAA ATTCCAGTGA TTCGCCAAGG AGATACTTCA 
AAGGTTTCAA TTGTGAGAGT CCACACCAAG GATGGCTCAG CCACTTCTGG AGAAGACTAT 
CACCCTGTGT CAGAAGAAAT TGAATTTAAG GAGGGAGAAA CCCAGCATAT TGTTAACATT 
GAAGTAATCT TCGATGGGAT CAGAGAGATG AGAGAGGCCT TCACTGTCCA CCTAAAACCC 
GATGAGAACA TGGTGGCAGA GACGCAGCCC ATTGTGTATA TAGAAGAAAT GAACAGCATG 
GCGGAAGTCA CTTTCCCATC TGTCCCTCAA ATTGTGTCAT TACTGATGTA TGATGACACT 
TCCAGAGCTA AAGATAGTGC AGGACCTGTG TCTGGCTATC CTGTTGTCTG TATTACAGCT 
TGCAACCCTA AATACTCAGA CTATGACAAA ACAGGTTCTA TTTGTGCAAG TGAAAATATC 
AATGACACCT TGACCCGGTA CCGATGGCTA ATTAGTGCAC CCGCTGGCCC TGATGGTGTG 
ACCAGCCCCA TGAGAGAAGT GGACTTTGAC ACCTTTTTTA CATCGTCCAA GATGATCACC 
TTGGATTCCA TATACTTCCA GCCCGGCTCC AGAGTGCAGT GTGCGGCCCG TGCTGTGAAT 
GCCAATGGCA ATGAGGGCCT GGAGCTGATG AGCCCTATTG TAACCATCGG CAGAGAAGAA 
GGTCTTTGTC AGCCCAGGGT GCCAGGGACA GTGGGAGCAG AGCCATTCTC AGCTAAGCTG 
CGCTACACAG GCCCCGAGGA CCCAGAGTAC ACGAACTTTA TCAAGCTCAC CGTCACGATG 
CCACACATAG ATGGTATGCT CCCTGTGATC TCCACTAAAG AGCTTTCCAA CTTTGAGCTC 
ACCCTCAGTC CTGATGGCTC AAGAGTTGGA AACCACCAGT GCTCCAATCT TCTAGATTAC 
ACTGAAGTGA AGACTCACCA TGGCTTCTTA ACTGATGCTA CGAAAAATCC AGAAGTAATT 
GGAGAGACTC ATCCTTACCA GTACAGCTCA TCTATCAGAG GTTCCAGTAC CTTACGCTTC 
TATCGAAACT TGAACCTGGA AGCCTGTTTA TGGGAGTTTG TTAGCTACTA TGACATGTCA 
GAACTTCTCA CCAACTGTGG AGGAACCATC GGGACAGACG GACAGGTCCT GAATCTGGTG 
CAGTCATATG TGACCCTTCG GGTGCCTCTG TATGTTTCGT ATGTGTTCCA TTCCCCCGTT 
GGCGTAGGGG GCTGGCAGCA TTTTGACTTG AAGTCGGAGC TCCGCCTGAC TTTCGTGTAT 
GACACTGCCA TCTTGTGGAA TGAGGGCATC GGCAGCCCAC CAGAAGCCGA GCTCCAAGGT 
TCTCTCTACC CGACTAGCAT GCGCATTGGT GAAGAGGGGC GCTTGGTTGT GAACTTCAAG 
ACAGAGGCTC AGTTCCATGG ATTGTTTGTG CTGTCTCATC CCGCATCCTT TACAAGCTCA 
ATGATCATGT CAGCTGATCA CCCGGGCCTG ACCTTCTCCT TGCGCCTCAT CAGGAGTGAA 
CCAACCTATA ACCAGCCAGT GCAGCAGTGG AGCTTTGTGT CTGACTTTGC AGTACGTGAC 
TATTCGGGGA CCTACACCGT GAAACTGCTA CCTTGCACTG CTCCATCGCA TCAGGAGTAC 
CACCTGCCAG TCACCTGCAG CCCCAGAGAG CCCATCACCT TTGACCTCGA CATACGATTC 
CAGCAGGTCA GTGATCCAGT GGCGGCTGAG TTTAGCTTGA ACACCCAAAT GTACCTCCTG 
TCCAAGAAGA GTCTCTGGTT GTCTGACGGA TCCATGGGAT TTGGGCAAGA GAGTGATGTT 
GCCTTCACAG AAGGTGATAT AATCTATGGT CGTGTCATGG TAGATCCTGT CCAGAATCTG 
GGTGACTCCT TTTACTGCAG CATTGAGAAG GTGTTCTTGT GCACTGGAGC TGATGGCTAT 
GTTCCCAAAT ATAATCCCAC AAATGCAGAA TATGGCTGCT TAGCCGACTC TCCTTCACTC 
TTATATAGAT TTAAAATTGT GGACAAAGCT CAACCAGAGA CACAAGCCAT CAGTTTTGGA 
AATGTCCTGT TTAATGCCAA ACTAGCAATG GATGATCCTG AAGCTATTCT CTTAGTGAAT 
CAGCCTGGAT CTGATGGATT TAAAGTCGAC TCCATGCCAC TCTTTCAGGT CGCTCTGGGC 
CGAGAATGGT ACATACACAC GGTCTACACA GTAAGATCAA AAGACAACGC CAATCGAGGC 
ATTGGCAAAA GAAGCACTGA GCACCAGCAC CACTCCTTAG TGAGACCAGG GAAGCCCCAG 
GCAACTAGCA AGGGCCGGAA GAAGAGGGAG ATCAGGAGCC CACCCCCACT GGCCTGGCAA 
ATAGGTGCTG AGAACAACCG GGGAACAAAC ATCCAGCACA TTGCCCTGGA CCGCAGCAGC 
CGGAAGCTGA TCCCCCAAGG CCGAGTTCCT CCTGACGGCG TCCTCCCCCG GGAGCTCAAC 
CGTCCCAGCT CTGAGGTCAG CCTGGTCACA GTCGTTGGCG GCATCGCGGT GGGATTGCTA 
ACCCTCTGCC TTGCTGCCAT CACAGTGATG ATGTGCAAGA GCAAGAAGGG CTCCAGGAGA 
AAGGATGCTC TAAAGTGCTC AGGCAGCAGC AAGCCCATGA TGCCTCCGCA GAGCCACCAC 
TGTGACAGCT CAGAGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM2 ( XM_543127.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_543127.3 Canis lupus familiaris FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

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CloneID	OCe25340
Clone ID Related Accession (Same CDS sequence)	XM_543127.5 , XM_543127.6
Accession Version	XM_543127.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	FRAS1-related extracellular matrix protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_543127.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCGG CCGGGGCCCC CGGGATCGCG TCCCGCGCCG CCACCGGTGC TCAGCCGGTG 
CCCCGGCTGC CGCCTCCCCT GCTGTTGCTA CTGCCGCTTC TCCTGTCCCC GGTAAGCCGC 
GCCCGGGCAC AGCCCGCTGC CCCGGGCCGG GAGCTGCTGT CCCCGGATCC CGCGGGCGCT 
GCGGGGGTCC TCGCCGAGGA GGCCGTGGTG CTGGCGAACC GGGGGCTCCG GGTGCCCTTC 
GGCCGCGAGG TCTGGCTGGA TCCGCGGCGC GACCTGGTGC TGCAGGTGCA GCCGGGGGAC 
CGCTGCGCGG TGACCGTGCT GGACAGTGAC GCGCTGGCGC AGAGGCCGGG CCGCCTGAGC 
CCCAAGCGCT TCCCGTGCGA CTTCGGCCCC GGGGAGGTGC GCTACTCGCA CCTGGGCGCT 
CGCAGCCCGT CGCGGGACCG CGTGCGGCTG CAGCTGCGCT ACGACGCGCC GGGAGGGGCC 
GCAGTGCTAC CGTTGGCGCT GGAGGTGGAG GTGGTCTTCA CCCAGCTGGA GGTCGTGACT 
CGGAACCTGC CCCTCGTCGT GGAGGAGCTG CTGGGGACCA GCAACGCCCT GGACGCGCGG 
AGCCTGGAGT TCGCCTACCA GCCCGAGACC GAGGAGTGTC GCGTGGGCAT CCTGTCGGGC 
TTGAGCGCGC TGCCCCGCTA CGGGGAACTG CTGCGCTACC CGCAGGTTCC CGGTGGAGCC 
GGCGACGGGG GCGCCCAGGA GCCTCTCCTG ATGGACTGCA AGGCCTTCCA GGAGCTGGGC 
GTGCGCTACC GCCACACGGC CCCCGGCCGG TCGCCCAACA GGGACTGGGT GCCCATGGTG 
GTGGAGCTGC GCGCTCGAGG CTCGCCAGTG GGCAGGCCTG CTTTGAAACG GGAGCACTTC 
CAGGTGCTGG TGAGAATCCG AGGGGGGGCG GAGAACACTG CGCCCAAGCC CAGTTTTGTG 
GCCATGATGA TGGTGGAGGT GGATCAGTTT GTCCTGACCG CCCTGACCCC CGACATGCTG 
GCGGCGGAGG ATGCAGAGTC GCCCCCTGAC CTGCTGGTCT TCAACCTCAC CGCTCCCTTC 
CAGCTGGGCC AGGGCTACCT GGTAAGTACC GATGACCGCA GCCTGCCCCT CTCCTCCTTC 
ACTCAGAGGG ATCTGCGGCT CCTGAAGATC GCCTACCAGC CGCCCTCTGA GGACTCTGAC 
CGAGAGCGGC TCTTTGAACT GGAGTTGGAG GTAGTGGATC CAGAAGGAGC AGCCTCAGAC 
CCTTTTGCTT TCATGGTTGT GGTGAAGCCC ATGAACACGC TGGCTCCAGT GGTCACCCGG 
AACACAGGCC TCCTTCTCTA CGAGGGCCAG TCCCGGCCCC TCACAGGCCC CGCAGGCAGT 
GGACCACAAA ACTTGGTCAT CAGCGATGAG GATGACCTGG AGGCAGTGCA GCTGGAGGTG 
GTGGCTGGGC TCCGGCATGG TCACCTTGTC CTCCTGGGTG CCACCCACGG CAACTCGGCC 
CCTAAGACCT TTTCGGTGGC CGAGCTAGCG GCCGGCCAGG TGGTCTACCA TCATGATGAC 
AAAGATGGCT CACTGAGTGA CAACCTTGTA CTTCGAATGG TGGACGGAGG ACGCAGGCAC 
CAGGTGCAGT TCCTGTTTCC CATCACCTTA GTGCCTGTGG ATGACCAGCC ACCAGTCCTC 
AATGCTAACA CGGGGCTGAC ACTGGCGGAA GGTGAAACGG TGCCCATCCT GCCCCTTGCT 
CTAAGTGCAG CTGACATTGA CTCAGAGGAC TCTCTGCTAC TTTTTGTGCT GGAATCGCCC 
TTCTCAGCGA TAGGGCATCT GCTTCTCCGC CAAACTCATC CTCCGCACGA GGAGGAGGAG 
TTTACTGGGA GCCCTTGGAG GAAACAGGGA GAGTTCTATG AGAAAGCAGT GACAGAATGG 
CGGCAGCAGG ACATAAGAGA GGGGAGGCTG TTCTACAGGC ACTCGGGGCC CCATAGTCCT 
GGGCCAGTGA TTGACCAGTT CACGTTTCGC GTCCAAGATA ACCATGACCC CCCTAATCAG 
TCAGGACCGC ACAGGTTTGT GGTGCGCATT CATCCGGTGG ATCGCCTCCC TCCAGAGTTG 
GGCAGTGGCT GTCCCCTGCG GATGGTGGTG CAGGAGTCCC AGCTCACCCC GCTGAGGAAG 
AAGTGGCTGC GCTACACCGA CCTGGACACA GATGACCGGG AACTCCATTA CACGGTGACC 
CAGCCCCCCA TGGACACAGA TGAAAACCAC TCGCCAGCCC CCCTGGGCTC CCTGGTCCTA 
ACAGACCAGC CCTCGGTCGT GGTGGCCCAT TTCACCCAAG CCCAGATCAA CCACCATAAA 
ATTGCTTACA AACCTCCAGA TCAAGAACTA GGAGTGGCTG CCCGAGTGGC CCAGTTCCAG 
TTCCAGGTGG AAGACCAAGC TGGGAATGTG GCCCCAGGCA CCTTCACCCT TTACCTGCAG 
CCAGTGGACA ACCAACCACC TGAGATCCTC AACACTGGTT TCACTGTTCA GGAGAAGGGC 
CACCATGTCC TGAGCGAGAC CGAGTTGCGT GTGGATGACG TAGACACAGA CGTGGCACAC 
ATCTCTTTCA CTCTCACTCA GGCACCCAAA CACGGCCACG TGAGAGTGTC TGGACAGATT 
TTGCACGTAG GGGAGGTCTT CCACTTGGAA GATATAAAAC AGGGTCGGAT TTCCTATGCA 
CATAATGGAG ATGAGTCCCT CACTGACAGC TGCTCCTTGG AGGTCAGCGA CACGCACCAC 
GTGGTGCCAA TCACTCTCAG AGTGAACATC CAGCCAGTGG ATGATGAGGT GCCCATGCTG 
AGCCTTTCTG CTGGCACTCT GGGGTCATAT CTAGATGTTT TAGAAAATGG AGCTACCGAA 
ATCACTGCCA GTGTTATCAA GGGGACTGAT GAGGACACTG ATGACTTGAT GTTGACTTTC 
CTCTTGGAAG ATCCACCCTT ATATGGTGAA ATCCTGGTCA ACGGAGTTCC AGCAGAGCAG 
TTTACCCAAA GAGACATTTT AGAGGGCTCT GTCGTCTATG CCCACACTAG TGGTGAGATA 
GGCCTGTTGC CCAAAGGGGA CTCTTTTAAC CTGAGCCTGT CGGATATGTC TCAAGAATGG 
GTAATAGGTG GCAGTACTAT CCAAGGAGTC ACCGTGTGGG TGACCGTCCT CCCTGTCGAT 
AGCCAGGCTC CAGAAATTTC TATAGGTGAA CAGTTTATAG TTGTGGAAGG TGATAAGAGT 
GTTGTAACAT CAGCACATAT AAGTGCTGAA GATACCGATT CACTAAATGA TGACATCTTA 
TGCACTATAG TTATTCAGCC TACGTCCGGT TATGTTGAAA ACATTTCTCC AGCCCCAGGC 
TCTGAGAAGT CAAGAGCAGG AGTTGCCATA AGTGCCTTTA CCGTGAAAGA CCTCAGGCAG 
AGTCATATTA ACTATGTCCA GAGTGTCCAT AAAGGGGTAG AACCTGTGGA AGATCGATTT 
ATATTTCGTT GCTCTGATGG CATTAACTTT TCAGAGAGAC ATTTATTCCC CATTGTCATC 
ATTCCCACCA ATGATGAACA GCCAGAAATA TTTATGAGAG AATTTATGGT GATGGAAGGC 
ATGAGTCTGG TGATCGACAC GCCCATCCTC AATGCTGCTG ATGCTGACAT TCCCCCCGAT 
GACTTAACTT TTACTGTTAC CCAATTTCCT ACTCGCGGTC ACATTGTGAA CCAGCTGATA 
AATGGCACAG TTTTGGTTGA AAGCTTTACC TTGGACCAGA TCATAGAGAG TTCTAGCATT 
ATTTATGAGC ATGATGACTC TGAGACCCAG GAAGACCGTT TTGTGATTAG ACTAACAGAT 
GGTAGGCATT CAGTGGAAAG GACGGTCCCC ATTATTGTTA TCCCAGTTGA TGATGAGACC 
CCCAGAATGA CCATCAATAA TGGACTAGAA ATAGAAATTG GGGAAACCAA AATTATCAAC 
AACAAAATAT TAATGGCAAC TGATTTAGAT TCGGAAGACA AATCTTTGGT TTATGTTATT 
CGTTATGGGC CAGGACATGG CTTATTGCAG AGGCAAAAAC CTACAGGTGC CTTTGAAAAT 
ATCACACTCG GCATGAATTT TACCCAGGAT GAAGTGGACA GAAACTTAAT TCAGTATGTC 
CATGTTGGAC AAGAGGGCAT TCGGGACCTT ATTAAATTTG ATGTGACTGA CGGAATAAAC 
GCCCTGATAG ATCGCTACTT TTACATTTCC GTCGGGAGTA TTGACACTGT CTTCCCAGAT 
GTGATAAGCA AGGGGGTATC CTTGAAAGAA GGAGGCAAAG TCACCCTCAC AACAGACCTC 
CTAAGCACAA GTGACCTGAA CAGTCCTGAT GAAAACTTAG TTTTCACCAT CACCAGGGCT 
CCCACGAGAG GTCACTTAGA GTGCACAGAT CAACGTGGAG CTTCCATCAT GTCTTTCACT 
CAGCTCCAAC TGGCTGGCAA CAAAATCTAC TACATCCACA CAGCTGATGA TGAGGTCAAG 
ATGGACAGTT TTGAGTTTCA AGTCACGGAT GGACGTAACC CTGTCTTCCG GACATTCCGT 
ATCTCCATTA GTGATGTGGA TAACAAAAAG CCAGTAGTCA CCATCCATAA CCTGGTTGTC 
AGCGAGGGTG AAAACAAGCT GATCACTCCC TTTGAACTCA CTGCCGAAGA CCCAGATACT 
CCTGACAAAC TCCTGAAGTT CATCGTCACC CAGGTGCCTG TTCATGGTCA ACTCTTATTC 
AACAATACCA GACCTGCCAT GGTTTTTACC AAGCAAGACT TGAATGAAAA CTTAATCAGC 
TACAGACATG ATGGCACCGA GTCAAGCGAA GACAGCTTCT CCTTCACGGT GACTGACAGT 
TCTCATACAG AGTTCTATGT CTTCCCTGAT ACAATATTTG AAACGAGGAG ACCCCAAGTG 
ATGAAAATCC AGGTCTTAGC TGTTGACGAT AGCATCCCCC AGATTGCAGT GAACAAAGGG 
GCTTCCACGC TTCGCCCTCT GGCTACCGGC CACTTGGGGT TCATGATCAC AAGCAAAGTA 
TTGAAAGCAG AGGACAGAGA CAGCTTACGT TTTTCTCTCA GGTTTATTGT GACAGGAGCT 
CCTCAACATG GGTATCTCCT CAATCTGGAC AGAGGCAACC ACAGTGTCAC TCAGTTTACA 
CAAGGTGACA TTGATGACAT GAAAATATGC TACATTTTAA AAGAGGGGGC CAATGCCACA 
AGTGATGTGT TCCATTTTAC AGTTGAAGAT GGTGGAGGAA ACAAATTAAC CAAGCAACAT 
TTTCGTCTGA ATTGGGCATG GATCTCCTTT GAAAAGGAAT ATTACCTGAT CAATGAGGAC 
TCCAAATTTC TAGATGTCGT TCTTAAACGT AGAGGGTTTT TGGGAGAGAC TTCATTTATA 
AGTATTGGCA CAAGAGATGG GACTGCAGAA AAAGACAAAG ACTTCAAGGG CAAAGCACAG 
AAACAAGTAC AGTTCAACCC AGGCCAGACC AGGGCAACCT GGAAAGTGCG GATCATGAGC 
GATGGGGAGC ATGAGCAGTC GGAGACCTTC CAGGTGGTTC TCTCAGAGCC GGTGCTGGCT 
GCTTTGGAGT TTCCCACAGT GACCACAGTG GAGATCATTG ACCCAGGAGA TGAGTCAACT 
GTATTTATTC CCCAGTCTGA ATACTCTGTT GAAGAAGACG TCGGTGAGCT GTTCATTCCC 
ATTAGGAGGA GTGGAGACGT GAGCCAGGAG CTGATGGTGA TCTGTTACAC ACAACAAGGA 
ACAGCAGGCG GAACTGTACC AACATCAGTG CTGTCCTATT CAGACTACAT ATCCAGGCCG 
GAGGACCACA CCAGTGTCAT CCGCTTTGAC AAAGATGAAC ACGAGAAAAT GTGTCGCATC 
GTTGTTATTG ATGACTCCCT GTATGAAGAG GAGGAAACAT TCCATGTCCT TCTGAGCATG 
CCCATGGGAG GGAGGATTGG ATCAGAGTTC CCAGGGACTC AGGTTACAAT CATCCCTGAC 
AAAGATGATG AACCTGTCTT CTACTTTGGT GATGTCGAGT ACCTGGTGGA CGAGAGCGCC 
GGCTATGTGG AATTGCGGGT GTGGAGAACA GGCACCGACC TGTCCAGGTC TTCCAGTGTC 
ACCGTGAGGT CTCGGAAAAC AGATCCTCCC TCTGCTGATG CCGGAACAGA TTATGTGGGA 
ATCAGTCGGA ATTTAGATTT TGCACCTGGA GTCAACATGC AGACTGTTCG TGTTATCATC 
CTGGATGATC TTGGGCAACC AGTGCTGGAG GGAATTGAGA AATTTGAACT GGTGCTTCGT 
ATGCCTATGA ACGCTGCCCT CGGCGAGCCC AGCAAAGCCA CGGTTTCCAT AAATGATTCT 
GTCTCCGACT TGCCTAAGAT GCAGTTCAAA GAACGAGTAT ATACTGGCAA TGAAAACGAT 
GGGCAGATAG TTGCAATGAT CCACAGGAGT GGAGACATTC ACTATAGGTC TTCAGTGAGA 
TGCTACACAC GACAGGGGTC TGCACAGGTG ATGACGGACT TTGAGGAACG TCCAAACATT 
GATAGCTCTG TCATCCTGTT CCTCCCTGGT GAGACTGAAA AGCCTTGTGT ACTTGAGCTG 
ATGGATGACA AACTCTATGA AGAGGTAGAG GAACTCCGCC TGGTTCTTGG AACCCCAAGT 
AGCGACTCCC CCTTTGGGGC TGCAGTTGGG GAACAAAATG AAACTCTGAT AAGGATCCAA 
GATGATGCTG ATAAGACTGT TATTAAATTT GGAGAAACCA GATTTAGCGT GAGTGAACCC 
AAGGAACCAG GACAGTCGGT GGTTGTAAAA ATTCCAGTGA TTCGCCAAGG AGATACTTCA 
AAGGTTTCAA TTGTGAGAGT CCACACCAAG GATGGCTCAG CCACTTCTGG AGAAGACTAT 
CACCCTGTGT CAGAAGAAAT TGAATTTAAG GAGGGAGAAA CCCAGCATAT TGTTAACATT 
GAAGTAATCT TCGATGGGAT CAGAGAGATG AGAGAGGCCT TCACTGTCCA CCTAAAACCC 
GATGAGAACA TGGTGGCAGA GACGCAGCCC ATTGTGTATA TAGAAGAAAT GAACAGCATG 
GCGGAAGTCA CTTTCCCATC TGTCCCTCAA ATTGTGTCAT TACTGATGTA TGATGACACT 
TCCAGAGCTA AAGATAGTGC AGGACCTGTG TCTGGCTATC CTGTTGTCTG TATTACAGCT 
TGCAACCCTA AATACTCAGA CTATGACAAA ACAGGTTCTA TTTGTGCAAG TGAAAATATC 
AATGACACCT TGACCCGGTA CCGATGGCTA ATTAGTGCAC CCGCTGGCCC TGATGGTGTG 
ACCAGCCCCA TGAGAGAAGT GGACTTTGAC ACCTTTTTTA CATCGTCCAA GATGATCACC 
TTGGATTCCA TATACTTCCA GCCCGGCTCC AGAGTGCAGT GTGCGGCCCG TGCTGTGAAT 
GCCAATGGCA ATGAGGGCCT GGAGCTGATG AGCCCTATTG TAACCATCGG CAGAGAAGAA 
GGTCTTTGTC AGCCCAGGGT GCCAGGGACA GTGGGAGCAG AGCCATTCTC AGCTAAGCTG 
CGCTACACAG GCCCCGAGGA CCCAGAGTAC ACGAACTTTA TCAAGCTCAC CGTCACGATG 
CCACACATAG ATGGTATGCT CCCTGTGATC TCCACTAAAG AGCTTTCCAA CTTTGAGCTC 
ACCCTCAGTC CTGATGGCTC AAGAGTTGGA AACCACCAGT GCTCCAATCT TCTAGATTAC 
ACTGAAGTGA AGACTCACCA TGGCTTCTTA ACTGATGCTA CGAAAAATCC AGAAGTAATT 
GGAGAGACTC ATCCTTACCA GTACAGCTCA TCTATCAGAG GTTCCAGTAC CTTACGCTTC 
TATCGAAACT TGAACCTGGA AGCCTGTTTA TGGGAGTTTG TTAGCTACTA TGACATGTCA 
GAACTTCTCA CCAACTGTGG AGGAACCATC GGGACAGACG GACAGGTCCT GAATCTGGTG 
CAGTCATATG TGACCCTTCG GGTGCCTCTG TATGTTTCGT ATGTGTTCCA TTCCCCCGTT 
GGCGTAGGGG GCTGGCAGCA TTTTGACTTG AAGTCGGAGC TCCGCCTGAC TTTCGTGTAT 
GACACTGCCA TCTTGTGGAA TGAGGGCATC GGCAGCCCAC CAGAAGCCGA GCTCCAAGGT 
TCTCTCTACC CGACTAGCAT GCGCATTGGT GAAGAGGGGC GCTTGGTTGT GAACTTCAAG 
ACAGAGGCTC AGTTCCATGG ATTGTTTGTG CTGTCTCATC CCGCATCCTT TACAAGCTCA 
ATGATCATGT CAGCTGATCA CCCGGGCCTG ACCTTCTCCT TGCGCCTCAT CAGGAGTGAA 
CCAACCTATA ACCAGCCAGT GCAGCAGTGG AGCTTTGTGT CTGACTTTGC AGTACGTGAC 
TATTCGGGGA CCTACACCGT GAAACTGCTA CCTTGCACTG CTCCATCGCA TCAGGAGTAC 
CACCTGCCAG TCACCTGCAG CCCCAGAGAG CCCATCACCT TTGACCTCGA CATACGATTC 
CAGCAGGTCA GTGATCCAGT GGCGGCTGAG TTTAGCTTGA ACACCCAAAT GTACCTCCTG 
TCCAAGAAGA GTCTCTGGTT GTCTGACGGA TCCATGGGAT TTGGGCAAGA GAGTGATGTT 
GCCTTCACAG AAGGTGATAT AATCTATGGT CGTGTCATGG TAGATCCTGT CCAGAATCTG 
GGTGACTCCT TTTACTGCAG CATTGAGAAG GTGTTCTTGT GCACTGGAGC TGATGGCTAT 
GTTCCCAAAT ATAATCCCAC AAATGCAGAA TATGGCTGCT TAGCCGACTC TCCTTCACTC 
TTATATAGAT TTAAAATTGT GGACAAAGCT CAACCAGAGA CACAAGCCAT CAGTTTTGGA 
AATGTCCTGT TTAATGCCAA ACTAGCAATG GATGATCCTG AAGCTATTCT CTTAGTGAAT 
CAGCCTGGAT CTGATGGATT TAAAGTCGAC TCCATGCCAC TCTTTCAGGT CGCTCTGGGC 
CGAGAATGGT ACATACACAC GGTCTACACA GTAAGATCAA AAGACAACGC CAATCGAGGC 
ATTGGCAAAA GAAGCACTGA GCACCAGCAC CACTCCTTAG TGAGACCAGG GAAGCCCCAG 
GCAACTAGCA AGGGCCGGAA GAAGAGGGAG ATCAGGAGCC CACCCCCACT GGCCTGGCAA 
ATAGGTGCTG AGAACAACCG GGGAACAAAC ATCCAGCACA TTGCCCTGGA CCGCAGCAGC 
CGGAAGCTGA TCCCCCAAGG CCGAGTTCCT CCTGACGGCG TCCTCCCCCG GGAGCTCAAC 
CGTCCCAGCT CTGAGGTCAG CCTGGTCACA GTCGTTGGCG GCATCGCGGT GGGATTGCTA 
ACCCTCTGCC TTGCTGCCAT CACAGTGATG ATGTGCAAGA GCAAGAAGGG CTCCAGGAGA 
AAGGATGCTC TAAAGTGCTC AGGCAGCAGC AAGCCCATGA TGCCTCCGCA GAGCCACCAC 
TGTGACAGCT CAGAGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_543127.3
CDS	102..9602
Translation	MRAAGAPGIASRAATGAQPVPRLPPPLLLLLPLLLSPVSRARAQPAAPGRELLSPDPAGAAGVLAEEAVVLANRGLRVPFGREVWLDPRRDLVLQVQPGDRCAVTVLDSDALAQRPGRLSPKRFPCDFGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAAVLPLALEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAYQPETEECRVGILSGLSALPRYGELLRYPQVPGGAGDGGAQEPLLMDCKAFQELGVRYRHTAPGRSPNRDWVPMVVELRARGSPVGRPALKREHFQVLVRIRGGAENTAPKPSFVAMMMVEVDQFVLTALTPDMLAAEDAESPPDLLVFNLTAPFQLGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDRERLFELELEVVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLLLYEGQSRPLTGPAGSGPQNLVISDEDDLEAVQLEVVAGLRHGHLVLLGATHGNSAPKTFSVAELAAGQVVYHHDDKDGSLSDNLVLRMVDGGRRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETVPILPLALSAADIDSEDSLLLFVLESPFSAIGHLLLRQTHPPHEEEEFTGSPWRKQGEFYEKAVTEWRQQDIREGRLFYRHSGPHSPGPVIDQFTFRVQDNHDPPNQSGPHRFVVRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELHYTVTQPPMDTDENHSPAPLGSLVLTDQPSVVVAHFTQAQINHHKIAYKPPDQELGVAARVAQFQFQVEDQAGNVAPGTFTLYLQPVDNQPPEILNTGFTVQEKGHHVLSETELRVDDVDTDVAHISFTLTQAPKHGHVRVSGQILHVGEVFHLEDIKQGRISYAHNGDESLTDSCSLEVSDTHHVVPITLRVNIQPVDDEVPMLSLSAGTLGSYLDVLENGATEITASVIKGTDEDTDDLMLTFLLEDPPLYGEILVNGVPAEQFTQRDILEGSVVYAHTSGEIGLLPKGDSFNLSLSDMSQEWVIGGSTIQGVTVWVTVLPVDSQAPEISIGEQFIVVEGDKSVVTSAHISAEDTDSLNDDILCTIVIQPTSGYVENISPAPGSEKSRAGVAISAFTVKDLRQSHINYVQSVHKGVEPVEDRFIFRCSDGINFSERHLFPIVIIPTNDEQPEIFMREFMVMEGMSLVIDTPILNAADADIPPDDLTFTVTQFPTRGHIVNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDRFVIRLTDGRHSVERTVPIIVIPVDDETPRMTINNGLEIEIGETKIINNKILMATDLDSEDKSLVYVIRYGPGHGLLQRQKPTGAFENITLGMNFTQDEVDRNLIQYVHVGQEGIRDLIKFDVTDGINALIDRYFYISVGSIDTVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPTRGHLECTDQRGASIMSFTQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSEGENKLITPFELTAEDPDTPDKLLKFIVTQVPVHGQLLFNNTRPAMVFTKQDLNENLISYRHDGTESSEDSFSFTVTDSSHTEFYVFPDTIFETRRPQVMKIQVLAVDDSIPQIAVNKGASTLRPLATGHLGFMITSKVLKAEDRDSLRFSLRFIVTGAPQHGYLLNLDRGNHSVTQFTQGDIDDMKICYILKEGANATSDVFHFTVEDGGGNKLTKQHFRLNWAWISFEKEYYLINEDSKFLDVVLKRRGFLGETSFISIGTRDGTAEKDKDFKGKAQKQVQFNPGQTRATWKVRIMSDGEHEQSETFQVVLSEPVLAALEFPTVTTVEIIDPGDESTVFIPQSEYSVEEDVGELFIPIRRSGDVSQELMVICYTQQGTAGGTVPTSVLSYSDYISRPEDHTSVIRFDKDEHEKMCRIVVIDDSLYEEEETFHVLLSMPMGGRIGSEFPGTQVTIIPDKDDEPVFYFGDVEYLVDESAGYVELRVWRTGTDLSRSSSVTVRSRKTDPPSADAGTDYVGISRNLDFAPGVNMQTVRVIILDDLGQPVLEGIEKFELVLRMPMNAALGEPSKATVSINDSVSDLPKMQFKERVYTGNENDGQIVAMIHRSGDIHYRSSVRCYTRQGSAQVMTDFEERPNIDSSVILFLPGETEKPCVLELMDDKLYEEVEELRLVLGTPSSDSPFGAAVGEQNETLIRIQDDADKTVIKFGETRFSVSEPKEPGQSVVVKIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHIVNIEVIFDGIREMREAFTVHLKPDENMVAETQPIVYIEEMNSMAEVTFPSVPQIVSLLMYDDTSRAKDSAGPVSGYPVVCITACNPKYSDYDKTGSICASENINDTLTRYRWLISAPAGPDGVTSPMREVDFDTFFTSSKMITLDSIYFQPGSRVQCAARAVNANGNEGLELMSPIVTIGREEGLCQPRVPGTVGAEPFSAKLRYTGPEDPEYTNFIKLTVTMPHIDGMLPVISTKELSNFELTLSPDGSRVGNHQCSNLLDYTEVKTHHGFLTDATKNPEVIGETHPYQYSSSIRGSSTLRFYRNLNLEACLWEFVSYYDMSELLTNCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWNEGIGSPPEAELQGSLYPTSMRIGEEGRLVVNFKTEAQFHGLFVLSHPASFTSSMIMSADHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLLPCTAPSHQEYHLPVTCSPREPITFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFTEGDIIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYNPTNAEYGCLADSPSLLYRFKIVDKAQPETQAISFGNVLFNAKLAMDDPEAILLVNQPGSDGFKVDSMPLFQVALGREWYIHTVYTVRSKDNANRGIGKRSTEHQHHSLVRPGKPQATSKGRKKREIRSPPPLAWQIGAENNRGTNIQHIALDRSSRKLIPQGRVPPDGVLPRELNRPSSEVSLVTVVGGIAVGLLTLCLAAITVMMCKSKKGSRRKDALKCSGSSKPMMPPQSHHCDSSEV

Target ORF information:

RefSeq Version	XM_543127.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_543127.6

ORF Insert Sequence:

ATGCGCGCGG CCGGGGCCCC CGGGATCGCG TCCCGCGCCG CCACCGGTGC TCAGCCGGTG 
CCCCGGCTGC CGCCTCCCCT GCTGTTGCTA CTGCCGCTTC TCCTGTCCCC GGTAAGCCGC 
GCCCGGGCAC AGCCCGCTGC CCCGGGCCGG GAGCTGCTGT CCCCGGATCC CGCGGGCGCT 
GCGGGGGTCC TCGCCGAGGA GGCCGTGGTG CTGGCGAACC GGGGGCTCCG GGTGCCCTTC 
GGCCGCGAGG TCTGGCTGGA TCCGCGGCGC GACCTGGTGC TGCAGGTGCA GCCGGGGGAC 
CGCTGCGCGG TGACCGTGCT GGACAGTGAC GCGCTGGCGC AGAGGCCGGG CCGCCTGAGC 
CCCAAGCGCT TCCCGTGCGA CTTCGGCCCC GGGGAGGTGC GCTACTCGCA CCTGGGCGCT 
CGCAGCCCGT CGCGGGACCG CGTGCGGCTG CAGCTGCGCT ACGACGCGCC GGGAGGGGCC 
GCAGTGCTAC CGTTGGCGCT GGAGGTGGAG GTGGTCTTCA CCCAGCTGGA GGTCGTGACT 
CGGAACCTGC CCCTCGTCGT GGAGGAGCTG CTGGGGACCA GCAACGCCCT GGACGCGCGG 
AGCCTGGAGT TCGCCTACCA GCCCGAGACC GAGGAGTGTC GCGTGGGCAT CCTGTCGGGC 
TTGAGCGCGC TGCCCCGCTA CGGGGAACTG CTGCGCTACC CGCAGGTTCC CGGTGGAGCC 
GGCGACGGGG GCGCCCAGGA GCCTCTCCTG ATGGACTGCA AGGCCTTCCA GGAGCTGGGC 
GTGCGCTACC GCCACACGGC CCCCGGCCGG TCGCCCAACA GGGACTGGGT GCCCATGGTG 
GTGGAGCTGC GCGCTCGAGG CTCGCCAGTG GGCAGGCCTG CTTTGAAACG GGAGCACTTC 
CAGGTGCTGG TGAGAATCCG AGGGGGGGCG GAGAACACTG CGCCCAAGCC CAGTTTTGTG 
GCCATGATGA TGGTGGAGGT GGATCAGTTT GTCCTGACCG CCCTGACCCC CGACATGCTG 
GCGGCGGAGG ATGCAGAGTC GCCCCCTGAC CTGCTGGTCT TCAACCTCAC CGCTCCCTTC 
CAGCTGGGCC AGGGCTACCT GGTAAGTACC GATGACCGCA GCCTGCCCCT CTCCTCCTTC 
ACTCAGAGGG ATCTGCGGCT CCTGAAGATC GCCTACCAGC CGCCCTCTGA GGACTCTGAC 
CGAGAGCGGC TCTTTGAACT GGAGTTGGAG GTAGTGGATC CAGAAGGAGC AGCCTCAGAC 
CCTTTTGCTT TCATGGTTGT GGTGAAGCCC ATGAACACGC TGGCTCCAGT GGTCACCCGG 
AACACAGGCC TCCTTCTCTA CGAGGGCCAG TCCCGGCCCC TCACAGGCCC CGCAGGCAGT 
GGACCACAAA ACTTGGTCAT CAGCGATGAG GATGACCTGG AGGCAGTGCA GCTGGAGGTG 
GTGGCTGGGC TCCGGCATGG TCACCTTGTC CTCCTGGGTG CCACCCACGG CAACTCGGCC 
CCTAAGACCT TTTCGGTGGC CGAGCTAGCG GCCGGCCAGG TGGTCTACCA TCATGATGAC 
AAAGATGGCT CACTGAGTGA CAACCTTGTA CTTCGAATGG TGGACGGAGG ACGCAGGCAC 
CAGGTGCAGT TCCTGTTTCC CATCACCTTA GTGCCTGTGG ATGACCAGCC ACCAGTCCTC 
AATGCTAACA CGGGGCTGAC ACTGGCGGAA GGTGAAACGG TGCCCATCCT GCCCCTTGCT 
CTAAGTGCAG CTGACATTGA CTCAGAGGAC TCTCTGCTAC TTTTTGTGCT GGAATCGCCC 
TTCTCAGCGA TAGGGCATCT GCTTCTCCGC CAAACTCATC CTCCGCACGA GGAGGAGGAG 
TTTACTGGGA GCCCTTGGAG GAAACAGGGA GAGTTCTATG AGAAAGCAGT GACAGAATGG 
CGGCAGCAGG ACATAAGAGA GGGGAGGCTG TTCTACAGGC ACTCGGGGCC CCATAGTCCT 
GGGCCAGTGA TTGACCAGTT CACGTTTCGC GTCCAAGATA ACCATGACCC CCCTAATCAG 
TCAGGACCGC ACAGGTTTGT GGTGCGCATT CATCCGGTGG ATCGCCTCCC TCCAGAGTTG 
GGCAGTGGCT GTCCCCTGCG GATGGTGGTG CAGGAGTCCC AGCTCACCCC GCTGAGGAAG 
AAGTGGCTGC GCTACACCGA CCTGGACACA GATGACCGGG AACTCCATTA CACGGTGACC 
CAGCCCCCCA TGGACACAGA TGAAAACCAC TCGCCAGCCC CCCTGGGCTC CCTGGTCCTA 
ACAGACCAGC CCTCGGTCGT GGTGGCCCAT TTCACCCAAG CCCAGATCAA CCACCATAAA 
ATTGCTTACA AACCTCCAGA TCAAGAACTA GGAGTGGCTG CCCGAGTGGC CCAGTTCCAG 
TTCCAGGTGG AAGACCAAGC TGGGAATGTG GCCCCAGGCA CCTTCACCCT TTACCTGCAG 
CCAGTGGACA ACCAACCACC TGAGATCCTC AACACTGGTT TCACTGTTCA GGAGAAGGGC 
CACCATGTCC TGAGCGAGAC CGAGTTGCGT GTGGATGACG TAGACACAGA CGTGGCACAC 
ATCTCTTTCA CTCTCACTCA GGCACCCAAA CACGGCCACG TGAGAGTGTC TGGACAGATT 
TTGCACGTAG GGGAGGTCTT CCACTTGGAA GATATAAAAC AGGGTCGGAT TTCCTATGCA 
CATAATGGAG ATGAGTCCCT CACTGACAGC TGCTCCTTGG AGGTCAGCGA CACGCACCAC 
GTGGTGCCAA TCACTCTCAG AGTGAACATC CAGCCAGTGG ATGATGAGGT GCCCATGCTG 
AGCCTTTCTG CTGGCACTCT GGGGTCATAT CTAGATGTTT TAGAAAATGG AGCTACCGAA 
ATCACTGCCA GTGTTATCAA GGGGACTGAT GAGGACACTG ATGACTTGAT GTTGACTTTC 
CTCTTGGAAG ATCCACCCTT ATATGGTGAA ATCCTGGTCA ACGGAGTTCC AGCAGAGCAG 
TTTACCCAAA GAGACATTTT AGAGGGCTCT GTCGTCTATG CCCACACTAG TGGTGAGATA 
GGCCTGTTGC CCAAAGGGGA CTCTTTTAAC CTGAGCCTGT CGGATATGTC TCAAGAATGG 
GTAATAGGTG GCAGTACTAT CCAAGGAGTC ACCGTGTGGG TGACCGTCCT CCCTGTCGAT 
AGCCAGGCTC CAGAAATTTC TATAGGTGAA CAGTTTATAG TTGTGGAAGG TGATAAGAGT 
GTTGTAACAT CAGCACATAT AAGTGCTGAA GATACCGATT CACTAAATGA TGACATCTTA 
TGCACTATAG TTATTCAGCC TACGTCCGGT TATGTTGAAA ACATTTCTCC AGCCCCAGGC 
TCTGAGAAGT CAAGAGCAGG AGTTGCCATA AGTGCCTTTA CCGTGAAAGA CCTCAGGCAG 
AGTCATATTA ACTATGTCCA GAGTGTCCAT AAAGGGGTAG AACCTGTGGA AGATCGATTT 
ATATTTCGTT GCTCTGATGG CATTAACTTT TCAGAGAGAC ATTTATTCCC CATTGTCATC 
ATTCCCACCA ATGATGAACA GCCAGAAATA TTTATGAGAG AATTTATGGT GATGGAAGGC 
ATGAGTCTGG TGATCGACAC GCCCATCCTC AATGCTGCTG ATGCTGACAT TCCCCCCGAT 
GACTTAACTT TTACTGTTAC CCAATTTCCT ACTCGCGGTC ACATTGTGAA CCAGCTGATA 
AATGGCACAG TTTTGGTTGA AAGCTTTACC TTGGACCAGA TCATAGAGAG TTCTAGCATT 
ATTTATGAGC ATGATGACTC TGAGACCCAG GAAGACCGTT TTGTGATTAG ACTAACAGAT 
GGTAGGCATT CAGTGGAAAG GACGGTCCCC ATTATTGTTA TCCCAGTTGA TGATGAGACC 
CCCAGAATGA CCATCAATAA TGGACTAGAA ATAGAAATTG GGGAAACCAA AATTATCAAC 
AACAAAATAT TAATGGCAAC TGATTTAGAT TCGGAAGACA AATCTTTGGT TTATGTTATT 
CGTTATGGGC CAGGACATGG CTTATTGCAG AGGCAAAAAC CTACAGGTGC CTTTGAAAAT 
ATCACACTCG GCATGAATTT TACCCAGGAT GAAGTGGACA GAAACTTAAT TCAGTATGTC 
CATGTTGGAC AAGAGGGCAT TCGGGACCTT ATTAAATTTG ATGTGACTGA CGGAATAAAC 
GCCCTGATAG ATCGCTACTT TTACATTTCC GTCGGGAGTA TTGACACTGT CTTCCCAGAT 
GTGATAAGCA AGGGGGTATC CTTGAAAGAA GGAGGCAAAG TCACCCTCAC AACAGACCTC 
CTAAGCACAA GTGACCTGAA CAGTCCTGAT GAAAACTTAG TTTTCACCAT CACCAGGGCT 
CCCACGAGAG GTCACTTAGA GTGCACAGAT CAACGTGGAG CTTCCATCAT GTCTTTCACT 
CAGCTCCAAC TGGCTGGCAA CAAAATCTAC TACATCCACA CAGCTGATGA TGAGGTCAAG 
ATGGACAGTT TTGAGTTTCA AGTCACGGAT GGACGTAACC CTGTCTTCCG GACATTCCGT 
ATCTCCATTA GTGATGTGGA TAACAAAAAG CCAGTAGTCA CCATCCATAA CCTGGTTGTC 
AGCGAGGGTG AAAACAAGCT GATCACTCCC TTTGAACTCA CTGCCGAAGA CCCAGATACT 
CCTGACAAAC TCCTGAAGTT CATCGTCACC CAGGTGCCTG TTCATGGTCA ACTCTTATTC 
AACAATACCA GACCTGCCAT GGTTTTTACC AAGCAAGACT TGAATGAAAA CTTAATCAGC 
TACAGACATG ATGGCACCGA GTCAAGCGAA GACAGCTTCT CCTTCACGGT GACTGACAGT 
TCTCATACAG AGTTCTATGT CTTCCCTGAT ACAATATTTG AAACGAGGAG ACCCCAAGTG 
ATGAAAATCC AGGTCTTAGC TGTTGACGAT AGCATCCCCC AGATTGCAGT GAACAAAGGG 
GCTTCCACGC TTCGCCCTCT GGCTACCGGC CACTTGGGGT TCATGATCAC AAGCAAAGTA 
TTGAAAGCAG AGGACAGAGA CAGCTTACGT TTTTCTCTCA GGTTTATTGT GACAGGAGCT 
CCTCAACATG GGTATCTCCT CAATCTGGAC AGAGGCAACC ACAGTGTCAC TCAGTTTACA 
CAAGGTGACA TTGATGACAT GAAAATATGC TACATTTTAA AAGAGGGGGC CAATGCCACA 
AGTGATGTGT TCCATTTTAC AGTTGAAGAT GGTGGAGGAA ACAAATTAAC CAAGCAACAT 
TTTCGTCTGA ATTGGGCATG GATCTCCTTT GAAAAGGAAT ATTACCTGAT CAATGAGGAC 
TCCAAATTTC TAGATGTCGT TCTTAAACGT AGAGGGTTTT TGGGAGAGAC TTCATTTATA 
AGTATTGGCA CAAGAGATGG GACTGCAGAA AAAGACAAAG ACTTCAAGGG CAAAGCACAG 
AAACAAGTAC AGTTCAACCC AGGCCAGACC AGGGCAACCT GGAAAGTGCG GATCATGAGC 
GATGGGGAGC ATGAGCAGTC GGAGACCTTC CAGGTGGTTC TCTCAGAGCC GGTGCTGGCT 
GCTTTGGAGT TTCCCACAGT GACCACAGTG GAGATCATTG ACCCAGGAGA TGAGTCAACT 
GTATTTATTC CCCAGTCTGA ATACTCTGTT GAAGAAGACG TCGGTGAGCT GTTCATTCCC 
ATTAGGAGGA GTGGAGACGT GAGCCAGGAG CTGATGGTGA TCTGTTACAC ACAACAAGGA 
ACAGCAGGCG GAACTGTACC AACATCAGTG CTGTCCTATT CAGACTACAT ATCCAGGCCG 
GAGGACCACA CCAGTGTCAT CCGCTTTGAC AAAGATGAAC ACGAGAAAAT GTGTCGCATC 
GTTGTTATTG ATGACTCCCT GTATGAAGAG GAGGAAACAT TCCATGTCCT TCTGAGCATG 
CCCATGGGAG GGAGGATTGG ATCAGAGTTC CCAGGGACTC AGGTTACAAT CATCCCTGAC 
AAAGATGATG AACCTGTCTT CTACTTTGGT GATGTCGAGT ACCTGGTGGA CGAGAGCGCC 
GGCTATGTGG AATTGCGGGT GTGGAGAACA GGCACCGACC TGTCCAGGTC TTCCAGTGTC 
ACCGTGAGGT CTCGGAAAAC AGATCCTCCC TCTGCTGATG CCGGAACAGA TTATGTGGGA 
ATCAGTCGGA ATTTAGATTT TGCACCTGGA GTCAACATGC AGACTGTTCG TGTTATCATC 
CTGGATGATC TTGGGCAACC AGTGCTGGAG GGAATTGAGA AATTTGAACT GGTGCTTCGT 
ATGCCTATGA ACGCTGCCCT CGGCGAGCCC AGCAAAGCCA CGGTTTCCAT AAATGATTCT 
GTCTCCGACT TGCCTAAGAT GCAGTTCAAA GAACGAGTAT ATACTGGCAA TGAAAACGAT 
GGGCAGATAG TTGCAATGAT CCACAGGAGT GGAGACATTC ACTATAGGTC TTCAGTGAGA 
TGCTACACAC GACAGGGGTC TGCACAGGTG ATGACGGACT TTGAGGAACG TCCAAACATT 
GATAGCTCTG TCATCCTGTT CCTCCCTGGT GAGACTGAAA AGCCTTGTGT ACTTGAGCTG 
ATGGATGACA AACTCTATGA AGAGGTAGAG GAACTCCGCC TGGTTCTTGG AACCCCAAGT 
AGCGACTCCC CCTTTGGGGC TGCAGTTGGG GAACAAAATG AAACTCTGAT AAGGATCCAA 
GATGATGCTG ATAAGACTGT TATTAAATTT GGAGAAACCA GATTTAGCGT GAGTGAACCC 
AAGGAACCAG GACAGTCGGT GGTTGTAAAA ATTCCAGTGA TTCGCCAAGG AGATACTTCA 
AAGGTTTCAA TTGTGAGAGT CCACACCAAG GATGGCTCAG CCACTTCTGG AGAAGACTAT 
CACCCTGTGT CAGAAGAAAT TGAATTTAAG GAGGGAGAAA CCCAGCATAT TGTTAACATT 
GAAGTAATCT TCGATGGGAT CAGAGAGATG AGAGAGGCCT TCACTGTCCA CCTAAAACCC 
GATGAGAACA TGGTGGCAGA GACGCAGCCC ATTGTGTATA TAGAAGAAAT GAACAGCATG 
GCGGAAGTCA CTTTCCCATC TGTCCCTCAA ATTGTGTCAT TACTGATGTA TGATGACACT 
TCCAGAGCTA AAGATAGTGC AGGACCTGTG TCTGGCTATC CTGTTGTCTG TATTACAGCT 
TGCAACCCTA AATACTCAGA CTATGACAAA ACAGGTTCTA TTTGTGCAAG TGAAAATATC 
AATGACACCT TGACCCGGTA CCGATGGCTA ATTAGTGCAC CCGCTGGCCC TGATGGTGTG 
ACCAGCCCCA TGAGAGAAGT GGACTTTGAC ACCTTTTTTA CATCGTCCAA GATGATCACC 
TTGGATTCCA TATACTTCCA GCCCGGCTCC AGAGTGCAGT GTGCGGCCCG TGCTGTGAAT 
GCCAATGGCA ATGAGGGCCT GGAGCTGATG AGCCCTATTG TAACCATCGG CAGAGAAGAA 
GGTCTTTGTC AGCCCAGGGT GCCAGGGACA GTGGGAGCAG AGCCATTCTC AGCTAAGCTG 
CGCTACACAG GCCCCGAGGA CCCAGAGTAC ACGAACTTTA TCAAGCTCAC CGTCACGATG 
CCACACATAG ATGGTATGCT CCCTGTGATC TCCACTAAAG AGCTTTCCAA CTTTGAGCTC 
ACCCTCAGTC CTGATGGCTC AAGAGTTGGA AACCACCAGT GCTCCAATCT TCTAGATTAC 
ACTGAAGTGA AGACTCACCA TGGCTTCTTA ACTGATGCTA CGAAAAATCC AGAAGTAATT 
GGAGAGACTC ATCCTTACCA GTACAGCTCA TCTATCAGAG GTTCCAGTAC CTTACGCTTC 
TATCGAAACT TGAACCTGGA AGCCTGTTTA TGGGAGTTTG TTAGCTACTA TGACATGTCA 
GAACTTCTCA CCAACTGTGG AGGAACCATC GGGACAGACG GACAGGTCCT GAATCTGGTG 
CAGTCATATG TGACCCTTCG GGTGCCTCTG TATGTTTCGT ATGTGTTCCA TTCCCCCGTT 
GGCGTAGGGG GCTGGCAGCA TTTTGACTTG AAGTCGGAGC TCCGCCTGAC TTTCGTGTAT 
GACACTGCCA TCTTGTGGAA TGAGGGCATC GGCAGCCCAC CAGAAGCCGA GCTCCAAGGT 
TCTCTCTACC CGACTAGCAT GCGCATTGGT GAAGAGGGGC GCTTGGTTGT GAACTTCAAG 
ACAGAGGCTC AGTTCCATGG ATTGTTTGTG CTGTCTCATC CCGCATCCTT TACAAGCTCA 
ATGATCATGT CAGCTGATCA CCCGGGCCTG ACCTTCTCCT TGCGCCTCAT CAGGAGTGAA 
CCAACCTATA ACCAGCCAGT GCAGCAGTGG AGCTTTGTGT CTGACTTTGC AGTACGTGAC 
TATTCGGGGA CCTACACCGT GAAACTGCTA CCTTGCACTG CTCCATCGCA TCAGGAGTAC 
CACCTGCCAG TCACCTGCAG CCCCAGAGAG CCCATCACCT TTGACCTCGA CATACGATTC 
CAGCAGGTCA GTGATCCAGT GGCGGCTGAG TTTAGCTTGA ACACCCAAAT GTACCTCCTG 
TCCAAGAAGA GTCTCTGGTT GTCTGACGGA TCCATGGGAT TTGGGCAAGA GAGTGATGTT 
GCCTTCACAG AAGGTGATAT AATCTATGGT CGTGTCATGG TAGATCCTGT CCAGAATCTG 
GGTGACTCCT TTTACTGCAG CATTGAGAAG GTGTTCTTGT GCACTGGAGC TGATGGCTAT 
GTTCCCAAAT ATAATCCCAC AAATGCAGAA TATGGCTGCT TAGCCGACTC TCCTTCACTC 
TTATATAGAT TTAAAATTGT GGACAAAGCT CAACCAGAGA CACAAGCCAT CAGTTTTGGA 
AATGTCCTGT TTAATGCCAA ACTAGCAATG GATGATCCTG AAGCTATTCT CTTAGTGAAT 
CAGCCTGGAT CTGATGGATT TAAAGTCGAC TCCATGCCAC TCTTTCAGGT CGCTCTGGGC 
CGAGAATGGT ACATACACAC GGTCTACACA GTAAGATCAA AAGACAACGC CAATCGAGGC 
ATTGGCAAAA GAAGCACTGA GCACCAGCAC CACTCCTTAG TGAGACCAGG GAAGCCCCAG 
GCAACTAGCA AGGGCCGGAA GAAGAGGGAG ATCAGGAGCC CACCCCCACT GGCCTGGCAA 
ATAGGTGCTG AGAACAACCG GGGAACAAAC ATCCAGCACA TTGCCCTGGA CCGCAGCAGC 
CGGAAGCTGA TCCCCCAAGG CCGAGTTCCT CCTGACGGCG TCCTCCCCCG GGAGCTCAAC 
CGTCCCAGCT CTGAGGTCAG CCTGGTCACA GTCGTTGGCG GCATCGCGGT GGGATTGCTA 
ACCCTCTGCC TTGCTGCCAT CACAGTGATG ATGTGCAAGA GCAAGAAGGG CTCCAGGAGA 
AAGGATGCTC TAAAGTGCTC AGGCAGCAGC AAGCCCATGA TGCCTCCGCA GAGCCACCAC 
TGTGACAGCT CAGAGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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FREM2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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