chd4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chd4a
Entrez Gene ID	558344
Full Name	chromodomain helicase DNA binding protein 4a
Synonyms	im:7143343,si:ch211-51m24.3
General protein information	Preferred Names chromodomain helicase DNA binding protein 4a Names chromodomain helicase DNA binding protein 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021468085.1	XP_021323760.1	chromodomain helicase DNA binding protein 4 isoform X1
XM_005159352.3	XP_005159409.1	chromodomain helicase DNA binding protein 4 isoform X2
NM_001044858.1	NP_001038323.1	chromodomain helicase DNA binding protein 4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chd4	NP_666091.1
Caenorhabditis elegans (roundworm)	chd-3	NP_510140.1
Danio rerio (zebrafish)	chd4a	NP_001038323.1
Canis lupus familiaris (dog)	CHD4	XP_867754.2
Danio rerio (zebrafish)	chd4b	XP_685699.4
Arabidopsis thaliana (thale cress)	PKL	NP_565587.1
Arabidopsis thaliana (thale cress)	PKR2	NP_194918.2
Homo sapiens (human)	CHD4	NP_001264.2
Bos taurus (cattle)	CHD4	NP_001193430.1
Gallus gallus (chicken)	CHD4	XP_003640489.2
Caenorhabditis elegans (roundworm)	let-418	NP_504523.1
Xenopus tropicalis (tropical clawed frog)	chd4	NP_001096166.1
	CHD4	XP_001107252.2
Rattus norvegicus (Norway rat)	Chd4	XP_006237457.1

Gene Ontology
Bibliography

Related articles in PubMed

Rescue of neural crest-derived phenotypes in a zebrafish CHARGE model by Sox10 downregulation.
Asad Z, Pandey A, Babu A, Sun Y, Shevade K, Kapoor S, Ullah I, Ranjan S, Scaria V, Bajpai R, Sachidanandan C
Human molecular genetics25(16)3539-3554(2016 08)

The chromatin remodeler chd5 is necessary for proper head development during embryogenesis of Danio rerio.
Bishop B, Ho KK, Tyler K, Smith A, Bonilla S, Leung YF, Ogas J
Biochimica et biophysica acta1849(8)1040-50(2015 Aug)

Specific NuRD components are required for fin regeneration in zebrafish.
Pfefferli C, M?ller F, Ja?wi?ska A, Wicky C
BMC biology1230(2014 Apr)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

Expansion of transducin subunit gene families in early vertebrate tetraploidizations.
Lagman D, Sundstr?m G, Ocampo Daza D, Abalo XM, Larhammar D
Genomics100(4)203-11(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chd4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chd4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23813	XM_021468085.1 Latest version!	Danio rerio chromodomain helicase DNA binding protein 4a (chd4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa34497	NM_001044858.1 Latest version!	Danio rerio chromodomain helicase DNA binding protein 4a (chd4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa23814	XM_005159352.3 Latest version!	Danio rerio chromodomain helicase DNA binding protein 4a (chd4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chd4a ( XM_021468085.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323760.1 Danio rerio chromodomain helicase DNA binding protein 4a (chd4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23813
Clone ID Related Accession (Same CDS sequence)	XM_021468085.1
Accession Version	XM_021468085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain helicase DNA binding protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA GTGAGGACGA TAGAGACGAT TTTGAACATC GGTCTGTGAT GCAAGAAGAG 
GACGACCTCG AAGAGGAGTT GTCAGAGAAT GATGCCCCAA AGGTGAAAAA GAAGAAGAAA 
GCCAAAAAAA GTAGCCGCGA GAGCAAAGGC AGCAAGAGAA ATCGCTCCCG CAGAGAGGAA 
ATTGGCATCA GTTCTCCAGA ACCAGGTGAA GGCCCAGATC TGGACGATGG TGAGGAGGAC 
CGCTCGGAAA GTGAAGGTAG TGACTACACA CCAGGCAGAA AAAAGAAGAA GAGAGCCAGC 
ACAGCGAAGG ATAAGAAGCG AAACAGTTCT GCCGCAGAGA GAAACTCTGC TGCCTCCAAA 
CGGAGGGAAC CGGAGGAAGA GGAAGACGAG GATGATGACG ATTCTGAACC AAAAAGTTCC 
TCTCAGCTTT TGGATACCTG GGGAATGGAG GACATCGACC ATATTTTCAC CGAGGAAGAT 
TATAGGACTC TGACCAACTA TAAAGCCTTC AGCCAGTTTG TCAGGCCTCT GATCGCAGCC 
AAGAACCCCA AAATTGCGGT TTCCAAGATG ATGATGGTTC TGGGAGCGAA GTGGCGGGAG 
TTCAGCACTA ACAATCCTCT GCGGGGCTCT GCTACATCTC TGGTGGCCGC TGCCTCTGTC 
GAAACGCCAC CGGAAACTCC AACCTCTGCA TCCGCAGCTC AGATCAGCCC CGAGTCCACG 
CCAGCAGCTC CCGTTCGCAA GGCCAAGACA AAAGAGGGCA AGGGTCCCAA TGCACGCAAG 
AAGTCCAAAC CAGCTCCTAA ACCCCAGGAG AAGAAGGTCA AAACCAAAAA GGTGGCTCCC 
TTGAAGATCA AACTAGGAGG CTTCAACAGC AAGAGGAAAC GCTCATCTAG TGAAGAGGAT 
GATGTTGATG TGGACAGTGA CTTTGACGAC GGCAGTATTA ACAGCGTGTC TGTTTCAAAT 
GGATCGAATA GTCGTAGCAG TCGCAGCAGC GCCAAAAAGA AGCCCAAAAA GAAGACGAAA 
AAGGCAGATG AAGATGGTGA TGGCTATGAG ACAGATCATC AGGATTACTG TGAGGTCTGC 
CAGCAGGGAG GTGAGATCAT TCTCTGTGAC ACCTGTCCAC GGGCCTATCA CATGGTCTGC 
TTGGACCCCG ACATGGAGAA GGCCCCTGAG GGTACCTGGA GCTGCCCACA CTGCGAGAAG 
ATGGGCATCC AGTGGGAGGC TCGTGAAGAC GCGTCTGAGG GCGAGGAGGA TAATGAGGCA 
GGAGGAGAGG CCGAAGAAGA CGACCATCAC ATGGAGTTCT GCAGAGTCTG TAAGGATGGC 
GGAGAGCTGC TGTGCTGCGA CTCATGCCCT TCGTCATACC ACATCCACTG CCTGAACCCA 
CCGCTGCCCG AGATCCCCAA CGGAGAGTGG ATCTGCCCTC GCTGCACTTG TCCCTCTATG 
AAAGGTAAAG TGCAGAAGAT TCTGACGTGG CGCTGGACTG AACCTCCACC GCCGACCCCT 
GTCCCACGTC CCTCTGACCT GCCAACTGAT GCTCCTGACC CCACACCTCT GGCTGGACGT 
CCTGAGAGAG AGTTCTTCGT TAAATGGCAG AATATGTCCT ACTGGCACTG CTCCTGGGTT 
ACTGAGCTGC AGCTGGAGAT TCACTGTCAG GTGATGTTCA GAAACTACCA GCGCAAGAAC 
GACATGGACG AACCTCCCCC CATAGACTTC GGAGGTGAAG GAGAAGAGGA GAAGAGCGAC 
AAGAGAAAGA AAAAAGACCC CACATACGCA CGGATGGAGG AGAAGTACTA CCGCTTCGGC 
ATCAAGATGG AGTGGATGGT CATTCATCGA ATCCTGAACC ACAGTGTGGA TAAGAAGAAC 
AACTGTCACT ACCTCATCAA ATGGAGGGAC TTAACATATG ATCAAGCCAC ATGGGAGTTG 
GAGGATATGG ATTTACCAGA CTATGACACG TATAAACTGC AGTACTGGAA CCACAGGGAG 
CTGATGATGG GTGATGAAGG TAAACCAGGA AAGAAGATCA AGATCAAAGG GAAGATGCGC 
AAACTGGACC GACCTCCAGA GAATCCTGTC GTGGATCCCA CCATTAAGTT TGAGCGTCAG 
CCGGATTATC TGGACACCAC AGGCGGAACG CTGCACCCAT ATCAGCTTGA AGGGCTCAAC 
TGGCTGCGCT TCTCTTGGGC TCAGGGAACC GACACCATCC TAGCAGACGA GATGGGGCTG 
GGAAAGACTG TGCAGACTGC TGTGTTCCTC TATTCACTGT ATAAAGAGGG TCATTCTAAA 
GGCCCGTTCC TGGTCAGTGC ACCGCTGTCC ACCATCATCA ACTGGGAGAG AGAGTTTGAG 
ATGTGGGCTC CGGACATGTA CGTAGTCACA TATGTGGGCG ACAAAGACAG CAGGGCCGTC 
ATCAGAGAAA ACGAGTTCTC CTTCGAAAAC AACGCCATCC GTGGTGGCAA GAAACCCTCC 
AAGATGAAGA AAGAAGCCTC CGTCAAGTTT CACGTGCTGT TGACCTCCTA TGAGTTGATC 
ACTATTGACA CAGCCGTTCT GGGCTCCATC GACTGGGCCT GTCTGGTTGT TGATGAGGCC 
CACAGACTGA AGAATAACCA GTCGAAGTTT TTCAGGATAC TGAACAACTA CCCGCTGCAG 
CATAAGCTGT TGTTGACAGG AACACCACTG CAAAACAACC TAGAAGAGCT TTTCCATCTG 
CTCAACTTCC TCACGCCGGA GAGATTCAGT AATCTTGAGG GTTTCTTGGA GGAGTTTGCG 
GACATCGCCA AGGAAGACCA GATCAAAAAG CTTCATGACA TGCTGGGACC ACACATGCTC 
AGGAGACTCA AAGCCGACGT GTTCAAGCAC ATGCCTTCAA AAACAGAGCT GATTGTGCGC 
GTGGAGCTCA GCCCCATGCA GAAGAAATAT TACAAGTTCA TCCTAACCCG AAACTTCGAG 
GCTCTGAACA CTCGTGGTGG AGGAAATCAG GTTTCTCTGC TGAATGTGGT CATGGACTTG 
AAGAAGTGCT GTAACCATCC TTATCTATTC CCTGCTGCTG CTATGGAAGC TGCCAAAATG 
CCCAATGGGA TGTATGAGGG TGGTGGCCTG ACTAAATCTT CTGGAAAACT GCTGCTACTG 
CAGAAGATGC TGCGCAAGCT GAAGGAGGGT GGACACAGGG TGCTCATCTT CTCTCAGATG 
ACCAAAATGT TGGACCTGCT GGAGGATTTC CTGGAGAATG AGGGCTACAA ATATGAACGT 
ATTGATGGAG GAATCACAGG AGGCATGAGG CAGGAAGCCA TTGACCGCTT TAACGCTCCT 
GGTGCTCCTC AGTTTGTTTT CCTGCTCTCG ACCAGAGCTG GTGGTTTGGG TATCAATCTG 
GCAACCGCAG ACACCGTCGT CATCTACGAT TCAGACTGGA ACCCTCACAA TGACATTCAG 
GCCTTCAGCA GAGCTCACAG GATTGGTCAA AACAAGAAGG TGATGATCTA CCGCTTTGTA 
ACCAAAGCTT CTGTAGAAGA GCGAATTACT CAGGTGGCCA AGAAGAAGAT GATGTTGACT 
CACTTGGTTG TGCGTCCTGG ATTGGGCTCA AAGACTGGTT CGATGTCTAA ACAGGAGCTC 
GATGACATTC TTAAATTCGG TACAGAGGAG CTATTTAAGG ACGAGGGAGA AGGAGAGAAC 
AAAGAAGGAC AAGAGGACAG CAGTGTGATT CACTATGATG ACAAAGCCAT CGATCGCCTG 
CTGGATCGTA ATCAGGATGC GACCGACGAC ACGGAGCTCC AGAGCATGAA CGAGTATCTC 
AGCTCCTTTA AAGTGGCGCA GTATGTGGTC AAGGATGAGG AAGAGGCGGA GGAGGAAGTT 
CAGAGGGAAA TCATCAAGCA GGAGGAAAGT GTGGATCCAG ATTACTGGGA GAAACTCCTC 
CGACACCATT ATGAGCAGCA GCAGGAGGAT TTGGCTCGTA ATCTGGGCAA GGGCAAGCGA 
ATCCGCAAAC AGGTCAACTA CAACGACGGC TCTCAGGAGG ACAGAGATTG GCAGGACGAT 
CAATCTGACG GCCAATCAGA CTATTCGGTT GCCTCAGAGG AAGGAGACGA GGACTTTGAC 
GAGCGATCAG AAGCCAATTC ACGGCGACCC AACAGGAAAG GCCTCAGAAA CGACAAAGAC 
AAACCACTGC CCCCCCTGCT GGCCAGAGTG AGCGGCAACA TAGAGGTGTT GGGTTTCAAT 
GCTCGTCAAA GGAAGGCTTT CCTGAATGCT GTGATGCGTT ATGGCATGCC ACCTCAGGAC 
GCCTTCACGA CACAGTGGCT GGTCAGAGAC CTGCGAGGCA AATCAGAGAG AGAGTTTAAG 
GCTTATGTCT CTCTCTTTAT GCGGCACTTG TGTGAACCTG GCGCAGATGG TGCTGAAAGC 
TTTGCAGATG GTGTTCCTCG AGAGGGACTT TCACGGCAGC ACGTCCTCAC GCGCATCGGT 
GTGATGTCGC TCATCCGAAA GAAGGTGCAA GAGTTTGAGC ATGTGAACGG TCAGTGGTCG 
ATGCCCTGGA TGATGGAGCT GAGCGAGAAT AAGACCACGT CCACCGCCGG CAGCCATCCA 
GACTCTCCCA GCAAAACCCC CACTACTGGC ACTCCATCAG ACACCCAGCC TAACACACCT 
GCTCCAGACG GTGTTCCCAA AGCAGAAGAT GCTGGTAAAG ATGCTGATAA AGAAAAGGTG 
AAAAATGGAG ACGGAGAGAA GGAGGCCAAT GAAAACAAAA ACAGTGAAGT CATTGCTGTT 
CCGGATGAAG ATGGCCGAGC TGCTTCTGCG TCTGAGGAGA AAGAGAAAGG CAAAGAGGAG 
AAGGAGAAGA CGCCTGAATC CTCCTCGGAG GCAGAAAAGG CTGATGGGAA GCAGGAAGAG 
AAGGAAGAAG AAAACGCCAA GTCGGGAGAC TCTGAGGAGA AAACTGATGA TACAGACAAA 
CCCAAAGGAT CTGAGGCCGA CAAGGAGACT TCAGACGCCA AAGGAGAAAA GAAGGATGAT 
GAGCCTGAGA AGATGGACAC AACTCCTGTT ACAGATGAGA AGAAAGGTCA GAAGGATGAG 
AAAGATTCTG GTAAAACAGA AGAAGCGGGA AAACTGCCTA ATGGAGAAAG TGCCAAAGAC 
AGCGCAGCGG GAGCAGGAAT TGAGGAGAGG AAGAAAGCCA AAACACGGTT CATGTTCAAC 
ATTGCAGATG GAGGATTCAC CGAATTGCAC TCCCTGTGGC AGAATGAGGA ACGAGCTGCT 
ACAGTCACTA AGAAAACCAA TGAGATCTGG CATCGTCGTC ATGACTACTG GCTCCTCGCA 
GGCATCATCC AACACGGGTA CGCTCGCTGG CAAGACATTC AGAATGACGT CCGGTTCGCC 
ATTCTTAACG AACCCTTCAA AGGAGAGATC AACAGAGGAA ACTTCCTGGA GATCAAAAAC 
AAGTTTCTTG CCCGGAGGTT CAAGTTGCTC GAGCAGGCTC TGGTGATTGA AGAACAGCTG 
CGCAGAGCTG CGTATCTGAA CATGACTGAA GACCCGTCTC ACCCCTCCAT GGCTCTCAAC 
ACACGCTTCA GTGAAGTGGA GTGTCTGGCC GAGTCCCACC AGCACCTGAG CAAAGAGTCC 
ATGTCTGGAA ACAAACCTGC CAACGCCGTC CTACATAAAG TACTGAAGCA GCTGGAGGAG 
TTGCTAAGTG ACATGAAAGC CGATGTCACT CGTCTCCCGG CAACCATCGC CAGGATACCG 
CCTGTTGCAG TGAGGCTGCA GATGTCTGAA AGAAACATTC TCAGCCGTTT GGCCAGCAGA 
GGACCAGACA CACAGAGTCA GCAGGTGCAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323760.1
CDS	347..6142
Translation	MSGSEDDRDDFEHRSVMQEEDDLEEELSENDAPKVKKKKKAKKSSRESKGSKRNRSRREEIGISSPEPGEGPDLDDGEEDRSESEGSDYTPGRKKKKRASTAKDKKRNSSAAERNSAASKRREPEEEEDEDDDDSEPKSSSQLLDTWGMEDIDHIFTEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPLRGSATSLVAAASVETPPETPTSASAAQISPESTPAAPVRKAKTKEGKGPNARKKSKPAPKPQEKKVKTKKVAPLKIKLGGFNSKRKRSSSEEDDVDVDSDFDDGSINSVSVSNGSNSRSSRSSAKKKPKKKTKKADEDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGTWSCPHCEKMGIQWEAREDASEGEEDNEAGGEAEEDDHHMEFCRVCKDGGELLCCDSCPSSYHIHCLNPPLPEIPNGEWICPRCTCPSMKGKVQKILTWRWTEPPPPTPVPRPSDLPTDAPDPTPLAGRPEREFFVKWQNMSYWHCSWVTELQLEIHCQVMFRNYQRKNDMDEPPPIDFGGEGEEEKSDKRKKKDPTYARMEEKYYRFGIKMEWMVIHRILNHSVDKKNNCHYLIKWRDLTYDQATWELEDMDLPDYDTYKLQYWNHRELMMGDEGKPGKKIKIKGKMRKLDRPPENPVVDPTIKFERQPDYLDTTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAVIRENEFSFENNAIRGGKKPSKMKKEASVKFHVLLTSYELITIDTAVLGSIDWACLVVDEAHRLKNNQSKFFRILNNYPLQHKLLLTGTPLQNNLEELFHLLNFLTPERFSNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKHMPSKTELIVRVELSPMQKKYYKFILTRNFEALNTRGGGNQVSLLNVVMDLKKCCNHPYLFPAAAMEAAKMPNGMYEGGGLTKSSGKLLLLQKMLRKLKEGGHRVLIFSQMTKMLDLLEDFLENEGYKYERIDGGITGGMRQEAIDRFNAPGAPQFVFLLSTRAGGLGINLATADTVVIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTKASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEGEGENKEGQEDSSVIHYDDKAIDRLLDRNQDATDDTELQSMNEYLSSFKVAQYVVKDEEEAEEEVQREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDGQSDYSVASEEGDEDFDERSEANSRRPNRKGLRNDKDKPLPPLLARVSGNIEVLGFNARQRKAFLNAVMRYGMPPQDAFTTQWLVRDLRGKSEREFKAYVSLFMRHLCEPGADGAESFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGQWSMPWMMELSENKTTSTAGSHPDSPSKTPTTGTPSDTQPNTPAPDGVPKAEDAGKDADKEKVKNGDGEKEANENKNSEVIAVPDEDGRAASASEEKEKGKEEKEKTPESSSEAEKADGKQEEKEEENAKSGDSEEKTDDTDKPKGSEADKETSDAKGEKKDDEPEKMDTTPVTDEKKGQKDEKDSGKTEEAGKLPNGESAKDSAAGAGIEERKKAKTRFMFNIADGGFTELHSLWQNEERAATVTKKTNEIWHRRHDYWLLAGIIQHGYARWQDIQNDVRFAILNEPFKGEINRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMTEDPSHPSMALNTRFSEVECLAESHQHLSKESMSGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLASRGPDTQSQQVQQ

Target ORF information:

RefSeq Version	XM_021468085.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 4a (chd4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468085.1

ORF Insert Sequence:

ATGTCCGGCA GTGAGGACGA TAGAGACGAT TTTGAACATC GGTCTGTGAT GCAAGAAGAG 
GACGACCTCG AAGAGGAGTT GTCAGAGAAT GATGCCCCAA AGGTGAAAAA GAAGAAGAAA 
GCCAAAAAAA GTAGCCGCGA GAGCAAAGGC AGCAAGAGAA ATCGCTCCCG CAGAGAGGAA 
ATTGGCATCA GTTCTCCAGA ACCAGGTGAA GGCCCAGATC TGGACGATGG TGAGGAGGAC 
CGCTCGGAAA GTGAAGGTAG TGACTACACA CCAGGCAGAA AAAAGAAGAA GAGAGCCAGC 
ACAGCGAAGG ATAAGAAGCG AAACAGTTCT GCCGCAGAGA GAAACTCTGC TGCCTCCAAA 
CGGAGGGAAC CGGAGGAAGA GGAAGACGAG GATGATGACG ATTCTGAACC AAAAAGTTCC 
TCTCAGCTTT TGGATACCTG GGGAATGGAG GACATCGACC ATATTTTCAC CGAGGAAGAT 
TATAGGACTC TGACCAACTA TAAAGCCTTC AGCCAGTTTG TCAGGCCTCT GATCGCAGCC 
AAGAACCCCA AAATTGCGGT TTCCAAGATG ATGATGGTTC TGGGAGCGAA GTGGCGGGAG 
TTCAGCACTA ACAATCCTCT GCGGGGCTCT GCTACATCTC TGGTGGCCGC TGCCTCTGTC 
GAAACGCCAC CGGAAACTCC AACCTCTGCA TCCGCAGCTC AGATCAGCCC CGAGTCCACG 
CCAGCAGCTC CCGTTCGCAA GGCCAAGACA AAAGAGGGCA AGGGTCCCAA TGCACGCAAG 
AAGTCCAAAC CAGCTCCTAA ACCCCAGGAG AAGAAGGTCA AAACCAAAAA GGTGGCTCCC 
TTGAAGATCA AACTAGGAGG CTTCAACAGC AAGAGGAAAC GCTCATCTAG TGAAGAGGAT 
GATGTTGATG TGGACAGTGA CTTTGACGAC GGCAGTATTA ACAGCGTGTC TGTTTCAAAT 
GGATCGAATA GTCGTAGCAG TCGCAGCAGC GCCAAAAAGA AGCCCAAAAA GAAGACGAAA 
AAGGCAGATG AAGATGGTGA TGGCTATGAG ACAGATCATC AGGATTACTG TGAGGTCTGC 
CAGCAGGGAG GTGAGATCAT TCTCTGTGAC ACCTGTCCAC GGGCCTATCA CATGGTCTGC 
TTGGACCCCG ACATGGAGAA GGCCCCTGAG GGTACCTGGA GCTGCCCACA CTGCGAGAAG 
ATGGGCATCC AGTGGGAGGC TCGTGAAGAC GCGTCTGAGG GCGAGGAGGA TAATGAGGCA 
GGAGGAGAGG CCGAAGAAGA CGACCATCAC ATGGAGTTCT GCAGAGTCTG TAAGGATGGC 
GGAGAGCTGC TGTGCTGCGA CTCATGCCCT TCGTCATACC ACATCCACTG CCTGAACCCA 
CCGCTGCCCG AGATCCCCAA CGGAGAGTGG ATCTGCCCTC GCTGCACTTG TCCCTCTATG 
AAAGGTAAAG TGCAGAAGAT TCTGACGTGG CGCTGGACTG AACCTCCACC GCCGACCCCT 
GTCCCACGTC CCTCTGACCT GCCAACTGAT GCTCCTGACC CCACACCTCT GGCTGGACGT 
CCTGAGAGAG AGTTCTTCGT TAAATGGCAG AATATGTCCT ACTGGCACTG CTCCTGGGTT 
ACTGAGCTGC AGCTGGAGAT TCACTGTCAG GTGATGTTCA GAAACTACCA GCGCAAGAAC 
GACATGGACG AACCTCCCCC CATAGACTTC GGAGGTGAAG GAGAAGAGGA GAAGAGCGAC 
AAGAGAAAGA AAAAAGACCC CACATACGCA CGGATGGAGG AGAAGTACTA CCGCTTCGGC 
ATCAAGATGG AGTGGATGGT CATTCATCGA ATCCTGAACC ACAGTGTGGA TAAGAAGAAC 
AACTGTCACT ACCTCATCAA ATGGAGGGAC TTAACATATG ATCAAGCCAC ATGGGAGTTG 
GAGGATATGG ATTTACCAGA CTATGACACG TATAAACTGC AGTACTGGAA CCACAGGGAG 
CTGATGATGG GTGATGAAGG TAAACCAGGA AAGAAGATCA AGATCAAAGG GAAGATGCGC 
AAACTGGACC GACCTCCAGA GAATCCTGTC GTGGATCCCA CCATTAAGTT TGAGCGTCAG 
CCGGATTATC TGGACACCAC AGGCGGAACG CTGCACCCAT ATCAGCTTGA AGGGCTCAAC 
TGGCTGCGCT TCTCTTGGGC TCAGGGAACC GACACCATCC TAGCAGACGA GATGGGGCTG 
GGAAAGACTG TGCAGACTGC TGTGTTCCTC TATTCACTGT ATAAAGAGGG TCATTCTAAA 
GGCCCGTTCC TGGTCAGTGC ACCGCTGTCC ACCATCATCA ACTGGGAGAG AGAGTTTGAG 
ATGTGGGCTC CGGACATGTA CGTAGTCACA TATGTGGGCG ACAAAGACAG CAGGGCCGTC 
ATCAGAGAAA ACGAGTTCTC CTTCGAAAAC AACGCCATCC GTGGTGGCAA GAAACCCTCC 
AAGATGAAGA AAGAAGCCTC CGTCAAGTTT CACGTGCTGT TGACCTCCTA TGAGTTGATC 
ACTATTGACA CAGCCGTTCT GGGCTCCATC GACTGGGCCT GTCTGGTTGT TGATGAGGCC 
CACAGACTGA AGAATAACCA GTCGAAGTTT TTCAGGATAC TGAACAACTA CCCGCTGCAG 
CATAAGCTGT TGTTGACAGG AACACCACTG CAAAACAACC TAGAAGAGCT TTTCCATCTG 
CTCAACTTCC TCACGCCGGA GAGATTCAGT AATCTTGAGG GTTTCTTGGA GGAGTTTGCG 
GACATCGCCA AGGAAGACCA GATCAAAAAG CTTCATGACA TGCTGGGACC ACACATGCTC 
AGGAGACTCA AAGCCGACGT GTTCAAGCAC ATGCCTTCAA AAACAGAGCT GATTGTGCGC 
GTGGAGCTCA GCCCCATGCA GAAGAAATAT TACAAGTTCA TCCTAACCCG AAACTTCGAG 
GCTCTGAACA CTCGTGGTGG AGGAAATCAG GTTTCTCTGC TGAATGTGGT CATGGACTTG 
AAGAAGTGCT GTAACCATCC TTATCTATTC CCTGCTGCTG CTATGGAAGC TGCCAAAATG 
CCCAATGGGA TGTATGAGGG TGGTGGCCTG ACTAAATCTT CTGGAAAACT GCTGCTACTG 
CAGAAGATGC TGCGCAAGCT GAAGGAGGGT GGACACAGGG TGCTCATCTT CTCTCAGATG 
ACCAAAATGT TGGACCTGCT GGAGGATTTC CTGGAGAATG AGGGCTACAA ATATGAACGT 
ATTGATGGAG GAATCACAGG AGGCATGAGG CAGGAAGCCA TTGACCGCTT TAACGCTCCT 
GGTGCTCCTC AGTTTGTTTT CCTGCTCTCG ACCAGAGCTG GTGGTTTGGG TATCAATCTG 
GCAACCGCAG ACACCGTCGT CATCTACGAT TCAGACTGGA ACCCTCACAA TGACATTCAG 
GCCTTCAGCA GAGCTCACAG GATTGGTCAA AACAAGAAGG TGATGATCTA CCGCTTTGTA 
ACCAAAGCTT CTGTAGAAGA GCGAATTACT CAGGTGGCCA AGAAGAAGAT GATGTTGACT 
CACTTGGTTG TGCGTCCTGG ATTGGGCTCA AAGACTGGTT CGATGTCTAA ACAGGAGCTC 
GATGACATTC TTAAATTCGG TACAGAGGAG CTATTTAAGG ACGAGGGAGA AGGAGAGAAC 
AAAGAAGGAC AAGAGGACAG CAGTGTGATT CACTATGATG ACAAAGCCAT CGATCGCCTG 
CTGGATCGTA ATCAGGATGC GACCGACGAC ACGGAGCTCC AGAGCATGAA CGAGTATCTC 
AGCTCCTTTA AAGTGGCGCA GTATGTGGTC AAGGATGAGG AAGAGGCGGA GGAGGAAGTT 
CAGAGGGAAA TCATCAAGCA GGAGGAAAGT GTGGATCCAG ATTACTGGGA GAAACTCCTC 
CGACACCATT ATGAGCAGCA GCAGGAGGAT TTGGCTCGTA ATCTGGGCAA GGGCAAGCGA 
ATCCGCAAAC AGGTCAACTA CAACGACGGC TCTCAGGAGG ACAGAGATTG GCAGGACGAT 
CAATCTGACG GCCAATCAGA CTATTCGGTT GCCTCAGAGG AAGGAGACGA GGACTTTGAC 
GAGCGATCAG AAGCCAATTC ACGGCGACCC AACAGGAAAG GCCTCAGAAA CGACAAAGAC 
AAACCACTGC CCCCCCTGCT GGCCAGAGTG AGCGGCAACA TAGAGGTGTT GGGTTTCAAT 
GCTCGTCAAA GGAAGGCTTT CCTGAATGCT GTGATGCGTT ATGGCATGCC ACCTCAGGAC 
GCCTTCACGA CACAGTGGCT GGTCAGAGAC CTGCGAGGCA AATCAGAGAG AGAGTTTAAG 
GCTTATGTCT CTCTCTTTAT GCGGCACTTG TGTGAACCTG GCGCAGATGG TGCTGAAAGC 
TTTGCAGATG GTGTTCCTCG AGAGGGACTT TCACGGCAGC ACGTCCTCAC GCGCATCGGT 
GTGATGTCGC TCATCCGAAA GAAGGTGCAA GAGTTTGAGC ATGTGAACGG TCAGTGGTCG 
ATGCCCTGGA TGATGGAGCT GAGCGAGAAT AAGACCACGT CCACCGCCGG CAGCCATCCA 
GACTCTCCCA GCAAAACCCC CACTACTGGC ACTCCATCAG ACACCCAGCC TAACACACCT 
GCTCCAGACG GTGTTCCCAA AGCAGAAGAT GCTGGTAAAG ATGCTGATAA AGAAAAGGTG 
AAAAATGGAG ACGGAGAGAA GGAGGCCAAT GAAAACAAAA ACAGTGAAGT CATTGCTGTT 
CCGGATGAAG ATGGCCGAGC TGCTTCTGCG TCTGAGGAGA AAGAGAAAGG CAAAGAGGAG 
AAGGAGAAGA CGCCTGAATC CTCCTCGGAG GCAGAAAAGG CTGATGGGAA GCAGGAAGAG 
AAGGAAGAAG AAAACGCCAA GTCGGGAGAC TCTGAGGAGA AAACTGATGA TACAGACAAA 
CCCAAAGGAT CTGAGGCCGA CAAGGAGACT TCAGACGCCA AAGGAGAAAA GAAGGATGAT 
GAGCCTGAGA AGATGGACAC AACTCCTGTT ACAGATGAGA AGAAAGGTCA GAAGGATGAG 
AAAGATTCTG GTAAAACAGA AGAAGCGGGA AAACTGCCTA ATGGAGAAAG TGCCAAAGAC 
AGCGCAGCGG GAGCAGGAAT TGAGGAGAGG AAGAAAGCCA AAACACGGTT CATGTTCAAC 
ATTGCAGATG GAGGATTCAC CGAATTGCAC TCCCTGTGGC AGAATGAGGA ACGAGCTGCT 
ACAGTCACTA AGAAAACCAA TGAGATCTGG CATCGTCGTC ATGACTACTG GCTCCTCGCA 
GGCATCATCC AACACGGGTA CGCTCGCTGG CAAGACATTC AGAATGACGT CCGGTTCGCC 
ATTCTTAACG AACCCTTCAA AGGAGAGATC AACAGAGGAA ACTTCCTGGA GATCAAAAAC 
AAGTTTCTTG CCCGGAGGTT CAAGTTGCTC GAGCAGGCTC TGGTGATTGA AGAACAGCTG 
CGCAGAGCTG CGTATCTGAA CATGACTGAA GACCCGTCTC ACCCCTCCAT GGCTCTCAAC 
ACACGCTTCA GTGAAGTGGA GTGTCTGGCC GAGTCCCACC AGCACCTGAG CAAAGAGTCC 
ATGTCTGGAA ACAAACCTGC CAACGCCGTC CTACATAAAG TACTGAAGCA GCTGGAGGAG 
TTGCTAAGTG ACATGAAAGC CGATGTCACT CGTCTCCCGG CAACCATCGC CAGGATACCG 
CCTGTTGCAG TGAGGCTGCA GATGTCTGAA AGAAACATTC TCAGCCGTTT GGCCAGCAGA 
GGACCAGACA CACAGAGTCA GCAGGTGCAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd4a ( NM_001044858.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038323.1 Danio rerio chromodomain helicase DNA binding protein 4a (chd4a), mRNA.

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CloneID	ODa34497
Clone ID Related Accession (Same CDS sequence)	NM_001044858.1
Accession Version	NM_001044858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-25
Organism	Danio rerio(zebrafish)
Product	chromodomain helicase DNA binding protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX004850.11. On or before Sep 1, 2006 this sequence version replaced XM_702225.1, XM_702226.1, XM_678924.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGGCA GTGAGGACGA TAGAGACGAT TTTGAACATC GGTCTGTGAT GCAAGAAGAG 
GACGACCTCG AAGAGGAGTT GTCAGAGAAT GATGCCCCAA AGGTGAAAAA GAAGAAGAAA 
GCCAAAAAAA GTAGCCGCGA GAGCAAAGGC AGCAAGAGAA ATCGCTCCCG CAGAGAGGAA 
ATTGGCATCA GTTCTCCAGA ACCAGGTGAA GGCCCAGATC TGGACGATGG TGAGGAGGAC 
CGCTCGGAAA GTGAAGGTAG TGACTACACA CCAGGCAGAA AAAAGAAGAA GAGAGCCAGC 
ACAGCGAAGG ATAAGAAGCG AAACAGTTCT GCCGCAGAGA GAAACTCTGC TGCCTCCAAA 
CGGAGGGAAC CGGAGGAAGA GGAAGACGAG GATGATGACG ATTCTGAACC AAAAAGTTCC 
TCTCAGCTTT TGGATACCTG GGGAATGGAG GACATCGACC ATATTTTCAC CGAGGAAGAT 
TATAGGACTC TGACCAACTA TAAAGCCTTC AGCCAGTTTG TCAGGCCTCT GATCGCAGCC 
AAGAACCCCA AAATTGCGGT TTCCAAGATG ATGATGGTTC TGGGAGCGAA GTGGCGGGAG 
TTCAGCACTA ACAATCCTCT GCGGGGCTCT GCTACATCTC TGGTGGCCGC TGCCTCTGTC 
GAAACGCCAC CGGAAACTCC AACCTCTGCA TCCGCAGCTC AGATCAGCCC CGAGTCCACG 
CCAGCAGCTC CCGTTCGCAA GGCCAAGACA AAAGAGGGCA AGGGTCCCAA TGCACGCAAG 
AAGTCCAAAC CAGCTCCTAA ACCCCAGGAG AAGAAGGTCA AAACCAAAAA GGTGGCTCCC 
TTGAAGATCA AACTAGGAGG CTTCAACAGC AAGAGGAAAC GCTCATCTAG TGAAGAGGAT 
GATGTTGATG TGGACAGTGA CTTTGACGAC GGCAGTATTA ACAGCGTGTC TGTTTCAAAT 
GGATCGAATA GTCGTAGCAG TCGCAGCAGC GCCAAAAAGA AGCCCAAAAA GAAGACGAAA 
AAGGCAGATG AAGATGGTGA TGGCTATGAG ACAGATCATC AGGATTACTG TGAGGTCTGC 
CAGCAGGGAG GTGAGATCAT TCTCTGTGAC ACCTGTCCAC GGGCCTATCA CATGGTCTGC 
TTGGACCCCG ACATGGAGAA GGCCCCTGAG GGTACCTGGA GCTGCCCACA CTGCGAGAAG 
ATGGGCATCC AGTGGGAGGC TCGTGAAGAC GCGTCTGAGG GCGAGGAGGA TAATGAGGCA 
GGAGGAGAGG CCGAAGAAGA CGACCATCAC ATGGAGTTCT GCAGAGTCTG TAAGGATGGC 
GGAGAGCTGC TGTGCTGCGA CTCATGCCCT TCGTCATACC ACATCCACTG CCTGAACCCA 
CCGCTGCCCG AGATCCCCAA CGGAGAGTGG ATCTGCCCTC GCTGCACTTG TCCCTCTATG 
AAAGGTAAAG TGCAGAAGAT TCTGACGTGG CGCTGGACTG AACCTCCACC GCCGACCCCT 
GTCCCACGTC CCTCTGACCT GCCAACTGAT GCTCCTGACC CCACACCTCT GGCTGGACGT 
CCTGAGAGAG AGTTCTTCGT TAAATGGCAG AATATGTCCT ACTGGCACTG CTCCTGGGTT 
ACTGAGCTGC AGCTGGAGAT TCACTGTCAG GTGATGTTCA GAAACTACCA GCGCAAGAAC 
GACATGGACG AACCTCCCCC CATAGACTTC GGAGGTGAAG GAGAAGAGGA GAAGAGCGAC 
AAGAGAAAGA AAAAAGACCC CACATACGCA CGGATGGAGG AGAAGTACTA CCGCTTCGGC 
ATCAAGATGG AGTGGATGGT CATTCATCGA ATCCTGAACC ACAGTGTGGA TAAGAAGAAC 
AACTGTCACT ACCTCATCAA ATGGAGGGAC TTAACATATG ATCAAGCCAC ATGGGAGTTG 
GAGGATATGG ATTTACCAGA CTATGACACG TATAAACTGC AGTACTGGAA CCACAGGGAG 
CTGATGATGG GTGATGAAGG TAAACCAGGA AAGAAGATCA AGATCAAAGG GAAGATGCGC 
AAACTGGACC GACCTCCAGA GAATCCTGTC GTGGATCCCA CCATTAAGTT TGAGCGTCAG 
CCGGATTATC TGGACACCAC AGGCGGAACG CTGCACCCAT ATCAGCTTGA AGGGCTCAAC 
TGGCTGCGCT TCTCTTGGGC TCAGGGAACC GACACCATCC TAGCAGACGA GATGGGGCTG 
GGAAAGACTG TGCAGACTGC TGTGTTCCTC TATTCACTGT ATAAAGAGGG TCATTCTAAA 
GGCCCGTTCC TGGTCAGTGC ACCGCTGTCC ACCATCATCA ACTGGGAGAG AGAGTTTGAG 
ATGTGGGCTC CGGACATGTA CGTAGTCACA TATGTGGGCG ACAAAGACAG CAGGGCCGTC 
ATCAGAGAAA ACGAGTTCTC CTTCGAAAAC AACGCCATCC GTGGTGGCAA GAAACCCTCC 
AAGATGAAGA AAGAAGCCTC CGTCAAGTTT CACGTGCTGT TGACCTCCTA TGAGTTGATC 
ACTATTGACA CAGCCGTTCT GGGCTCCATC GACTGGGCCT GTCTGGTTGT TGATGAGGCC 
CACAGACTGA AGAATAACCA GTCGAAGTTT TTCAGGATAC TGAACAACTA CCCGCTGCAG 
CATAAGCTGT TGTTGACAGG AACACCACTG CAAAACAACC TAGAAGAGCT TTTCCATCTG 
CTCAACTTCC TCACGCCGGA GAGATTCAGT AATCTTGAGG GTTTCTTGGA GGAGTTTGCG 
GACATCGCCA AGGAAGACCA GATCAAAAAG CTTCATGACA TGCTGGGACC ACACATGCTC 
AGGAGACTCA AAGCCGACGT GTTCAAGCAC ATGCCTTCAA AAACAGAGCT GATTGTGCGC 
GTGGAGCTCA GCCCCATGCA GAAGAAATAT TACAAGTTCA TCCTAACCCG AAACTTCGAG 
GCTCTGAACA CTCGTGGTGG AGGAAATCAG GTTTCTCTGC TGAATGTGGT CATGGACTTG 
AAGAAGTGCT GTAACCATCC TTATCTATTC CCTGCTGCTG CTATGGAAGC TGCCAAAATG 
CCCAATGGGA TGTATGAGGG TGGTGGCCTG ACTAAATCTT CTGGAAAACT GCTGCTACTG 
CAGAAGATGC TGCGCAAGCT GAAGGAGGGT GGACACAGGG TGCTCATCTT CTCTCAGATG 
ACCAAAATGT TGGACCTGCT GGAGGATTTC CTGGAGAATG AGGGCTACAA ATATGAACGT 
ATTGATGGAG GAATCACAGG AGGCATGAGG CAGGAAGCCA TTGACCGCTT TAACGCTCCT 
GGTGCTCCTC AGTTTGTTTT CCTGCTCTCG ACCAGAGCTG GTGGTTTGGG TATCAATCTG 
GCAACCGCAG ACACCGTCGT CATCTACGAT TCAGACTGGA ACCCTCACAA TGACATTCAG 
GCCTTCAGCA GAGCTCACAG GATTGGTCAA AACAAGAAGG TGATGATCTA CCGCTTTGTA 
ACCAAAGCTT CTGTAGAAGA GCGAATTACT CAGGTGGCCA AGAAGAAGAT GATGTTGACT 
CACTTGGTTG TGCGTCCTGG ATTGGGCTCA AAGACTGGTT CGATGTCTAA ACAGGAGCTC 
GATGACATTC TTAAATTCGG TACAGAGGAG CTATTTAAGG ACGAGGGAGA AGGAGAGAAC 
AAAGAAGGAC AAGAGGACAG CAGTGTGATT CACTATGATG ACAAAGCCAT CGATCGCCTG 
CTGGATCGTA ATCAGGATGC GACCGACGAC ACGGAGCTCC AGAGCATGAA CGAGTATCTC 
AGCTCCTTTA AAGTGGCGCA GTATGTGGAG GAGGAAGTTC AGAGGGAAAT CATCAAGCAG 
GAGGAAAGTG TGGATCCAGA TTACTGGGAG AAACTCCTCC GACACCATTA TGAGCAGCAG 
CAGGAGGATT TGGCTCGTAA TCTGGGCAAG GGCAAGCGAA TCCGCAAACA GGTCAACTAC 
AACGACGGCT CTCAGGAGGA CAGAGATTGG CAGGACGATC AATCTGACGG CCAATCAGAC 
TATTCGGTTG CCTCAGAGGA AGGAGACGAG GACTTTGACG AGCGATCAGA AGCCAATTCA 
CGGCGACCCA ACAGGAAAGG CCTCAGAAAC GACAAAGACA AACCACTGCC CCCCCTGCTG 
GCCAGAGTGA GCGGCAACAT AGAGGTGTTG GGTTTCAATG CTCGTCAAAG GAAGGCTTTC 
CTGAATGCTG TGATGCGTTA TGGCATGCCA CCTCAGGACG CCTTCACGAC ACAGTGGCTG 
GTCAGAGACC TGCGAGGCAA ATCAGAGAGA GAGTTTAAGG CTTATGTCTC TCTCTTTATG 
CGGCACTTGT GTGAACCTGG CGCAGATGGT GCTGAAAGCT TTGCAGATGG TGTTCCTCGA 
GAGGGACTTT CACGGCAGCA CGTCCTCACG CGCATCGGTG TGATGTCGCT CATCCGAAAG 
AAGGTGCAAG AGTTTGAGCA TGTGAACGGT CAGTGGTCGA TGCCCTGGAT GATGGAGCTG 
AGCGAGAATA AGACCACGTC CACCGCCGGC AGCCATCCAG ACTCTCCCAG CAAAACCCCC 
ACTACTGGCA CTCCATCAGA CACCCAGCCT AACACACCTG CTCCAGACGG TGTTCCCAAA 
GCAGAAGATG CTGGTAAAGA TGCTGATAAA GAAAAGGTGA AAAATGGAGA CGGAGAGAAG 
GAGGCCAATG AAAACAAAAA CAGTGAAGTC ATTGCTGTTC CGGATGAAGA TGGCCGAGCT 
GCTTCTGCGT CTGAGGAGAA AGAGAAAGGC AAAGAGGAGA AGGAGAAGAC GCCTGAATCC 
TCCTCGGAGG CAGAAAAGGC TGATGGGAAG CAGGAAGAGA AGGAAGAAGA AAACGCCAAG 
TCGGGAGACT CTGAGGAGAA AACTGATGAT ACAGACAAAC CCAAAGGATC TGAGGCCGAC 
AAGGAGACTT CAGACGCCAA AGGAGAAAAG AAGGATGATG AGCCTGAGAA GATGGACACA 
ACTCCTGTTA CAGATGAGAA GAAAGGTCAG AAGGATGAGA AAGATTCTGG TAAAACAGAA 
GAAGCGGGAA AACTGCCTAA TGGAGAAAGT GCCAAAGACA GCGCAGCGGG AGCAGGAATT 
GAGGAGAGGA AGAAAGCCAA AACACGGTTC ATGTTCAACA TTGCAGATGG AGGATTCACC 
GAATTGCACT CCCTGTGGCA GAATGAGGAA CGAGCTGCTA CAGTCACTAA GAAAACCAAT 
GAGATCTGGC ATCGTCGTCA TGACTACTGG CTCCTCGCAG GCATCATCCA GTATCTTTTA 
ATGCAACACG GGTACGCTCG CTGGCAAGAC ATTCAGAATG ACGTCCGGTT CGCCATTCTT 
AACGAACCCT TCAAAGGAGA GATCAACAGA GGAAACTTCC TGGAGATCAA AAACAAGTTT 
CTTGCCCGGA GGTTCAAGTT GCTCGAGCAG GCTCTGGTGA TTGAAGAACA GCTGCGCAGA 
GCTGCGTATC TGAACATGAC TGAAGACCCG TCTCACCCCT CCATGGCTCT CAACACACGC 
TTCAGTGAAG TGGAGTGTCT GGCCGAGTCC CACCAGCACC TGAGCAAAGA GTCCATGTCT 
GGAAACAAAC CTGCCAACGC CGTCCTACAT AAAGTACTGA AGCAGCTGGA GGAGTTGCTA 
AGTGACATGA AAGCCGATGT CACTCGTCTC CCGGCAACCA TCGCCAGGAT ACCGCCTGTT 
GCAGTGAGGC TGCAGATGTC TGAAAGAAAC ATTCTCAGCC GTTTGGCCAG CAGAGGACCA 
GACACACAGA GTCAGCAGGT GCAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038323.1
CDS	347..6136
Translation	MSGSEDDRDDFEHRSVMQEEDDLEEELSENDAPKVKKKKKAKKSSRESKGSKRNRSRREEIGISSPEPGEGPDLDDGEEDRSESEGSDYTPGRKKKKRASTAKDKKRNSSAAERNSAASKRREPEEEEDEDDDDSEPKSSSQLLDTWGMEDIDHIFTEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPLRGSATSLVAAASVETPPETPTSASAAQISPESTPAAPVRKAKTKEGKGPNARKKSKPAPKPQEKKVKTKKVAPLKIKLGGFNSKRKRSSSEEDDVDVDSDFDDGSINSVSVSNGSNSRSSRSSAKKKPKKKTKKADEDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGTWSCPHCEKMGIQWEAREDASEGEEDNEAGGEAEEDDHHMEFCRVCKDGGELLCCDSCPSSYHIHCLNPPLPEIPNGEWICPRCTCPSMKGKVQKILTWRWTEPPPPTPVPRPSDLPTDAPDPTPLAGRPEREFFVKWQNMSYWHCSWVTELQLEIHCQVMFRNYQRKNDMDEPPPIDFGGEGEEEKSDKRKKKDPTYARMEEKYYRFGIKMEWMVIHRILNHSVDKKNNCHYLIKWRDLTYDQATWELEDMDLPDYDTYKLQYWNHRELMMGDEGKPGKKIKIKGKMRKLDRPPENPVVDPTIKFERQPDYLDTTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAVIRENEFSFENNAIRGGKKPSKMKKEASVKFHVLLTSYELITIDTAVLGSIDWACLVVDEAHRLKNNQSKFFRILNNYPLQHKLLLTGTPLQNNLEELFHLLNFLTPERFSNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKHMPSKTELIVRVELSPMQKKYYKFILTRNFEALNTRGGGNQVSLLNVVMDLKKCCNHPYLFPAAAMEAAKMPNGMYEGGGLTKSSGKLLLLQKMLRKLKEGGHRVLIFSQMTKMLDLLEDFLENEGYKYERIDGGITGGMRQEAIDRFNAPGAPQFVFLLSTRAGGLGINLATADTVVIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTKASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEGEGENKEGQEDSSVIHYDDKAIDRLLDRNQDATDDTELQSMNEYLSSFKVAQYVEEEVQREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDGQSDYSVASEEGDEDFDERSEANSRRPNRKGLRNDKDKPLPPLLARVSGNIEVLGFNARQRKAFLNAVMRYGMPPQDAFTTQWLVRDLRGKSEREFKAYVSLFMRHLCEPGADGAESFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGQWSMPWMMELSENKTTSTAGSHPDSPSKTPTTGTPSDTQPNTPAPDGVPKAEDAGKDADKEKVKNGDGEKEANENKNSEVIAVPDEDGRAASASEEKEKGKEEKEKTPESSSEAEKADGKQEEKEEENAKSGDSEEKTDDTDKPKGSEADKETSDAKGEKKDDEPEKMDTTPVTDEKKGQKDEKDSGKTEEAGKLPNGESAKDSAAGAGIEERKKAKTRFMFNIADGGFTELHSLWQNEERAATVTKKTNEIWHRRHDYWLLAGIIQYLLMQHGYARWQDIQNDVRFAILNEPFKGEINRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMTEDPSHPSMALNTRFSEVECLAESHQHLSKESMSGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLASRGPDTQSQQVQQ

Target ORF information:

RefSeq Version	NM_001044858.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 4a (chd4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044858.1

ORF Insert Sequence:

ATGTCCGGCA GTGAGGACGA TAGAGACGAT TTTGAACATC GGTCTGTGAT GCAAGAAGAG 
GACGACCTCG AAGAGGAGTT GTCAGAGAAT GATGCCCCAA AGGTGAAAAA GAAGAAGAAA 
GCCAAAAAAA GTAGCCGCGA GAGCAAAGGC AGCAAGAGAA ATCGCTCCCG CAGAGAGGAA 
ATTGGCATCA GTTCTCCAGA ACCAGGTGAA GGCCCAGATC TGGACGATGG TGAGGAGGAC 
CGCTCGGAAA GTGAAGGTAG TGACTACACA CCAGGCAGAA AAAAGAAGAA GAGAGCCAGC 
ACAGCGAAGG ATAAGAAGCG AAACAGTTCT GCCGCAGAGA GAAACTCTGC TGCCTCCAAA 
CGGAGGGAAC CGGAGGAAGA GGAAGACGAG GATGATGACG ATTCTGAACC AAAAAGTTCC 
TCTCAGCTTT TGGATACCTG GGGAATGGAG GACATCGACC ATATTTTCAC CGAGGAAGAT 
TATAGGACTC TGACCAACTA TAAAGCCTTC AGCCAGTTTG TCAGGCCTCT GATCGCAGCC 
AAGAACCCCA AAATTGCGGT TTCCAAGATG ATGATGGTTC TGGGAGCGAA GTGGCGGGAG 
TTCAGCACTA ACAATCCTCT GCGGGGCTCT GCTACATCTC TGGTGGCCGC TGCCTCTGTC 
GAAACGCCAC CGGAAACTCC AACCTCTGCA TCCGCAGCTC AGATCAGCCC CGAGTCCACG 
CCAGCAGCTC CCGTTCGCAA GGCCAAGACA AAAGAGGGCA AGGGTCCCAA TGCACGCAAG 
AAGTCCAAAC CAGCTCCTAA ACCCCAGGAG AAGAAGGTCA AAACCAAAAA GGTGGCTCCC 
TTGAAGATCA AACTAGGAGG CTTCAACAGC AAGAGGAAAC GCTCATCTAG TGAAGAGGAT 
GATGTTGATG TGGACAGTGA CTTTGACGAC GGCAGTATTA ACAGCGTGTC TGTTTCAAAT 
GGATCGAATA GTCGTAGCAG TCGCAGCAGC GCCAAAAAGA AGCCCAAAAA GAAGACGAAA 
AAGGCAGATG AAGATGGTGA TGGCTATGAG ACAGATCATC AGGATTACTG TGAGGTCTGC 
CAGCAGGGAG GTGAGATCAT TCTCTGTGAC ACCTGTCCAC GGGCCTATCA CATGGTCTGC 
TTGGACCCCG ACATGGAGAA GGCCCCTGAG GGTACCTGGA GCTGCCCACA CTGCGAGAAG 
ATGGGCATCC AGTGGGAGGC TCGTGAAGAC GCGTCTGAGG GCGAGGAGGA TAATGAGGCA 
GGAGGAGAGG CCGAAGAAGA CGACCATCAC ATGGAGTTCT GCAGAGTCTG TAAGGATGGC 
GGAGAGCTGC TGTGCTGCGA CTCATGCCCT TCGTCATACC ACATCCACTG CCTGAACCCA 
CCGCTGCCCG AGATCCCCAA CGGAGAGTGG ATCTGCCCTC GCTGCACTTG TCCCTCTATG 
AAAGGTAAAG TGCAGAAGAT TCTGACGTGG CGCTGGACTG AACCTCCACC GCCGACCCCT 
GTCCCACGTC CCTCTGACCT GCCAACTGAT GCTCCTGACC CCACACCTCT GGCTGGACGT 
CCTGAGAGAG AGTTCTTCGT TAAATGGCAG AATATGTCCT ACTGGCACTG CTCCTGGGTT 
ACTGAGCTGC AGCTGGAGAT TCACTGTCAG GTGATGTTCA GAAACTACCA GCGCAAGAAC 
GACATGGACG AACCTCCCCC CATAGACTTC GGAGGTGAAG GAGAAGAGGA GAAGAGCGAC 
AAGAGAAAGA AAAAAGACCC CACATACGCA CGGATGGAGG AGAAGTACTA CCGCTTCGGC 
ATCAAGATGG AGTGGATGGT CATTCATCGA ATCCTGAACC ACAGTGTGGA TAAGAAGAAC 
AACTGTCACT ACCTCATCAA ATGGAGGGAC TTAACATATG ATCAAGCCAC ATGGGAGTTG 
GAGGATATGG ATTTACCAGA CTATGACACG TATAAACTGC AGTACTGGAA CCACAGGGAG 
CTGATGATGG GTGATGAAGG TAAACCAGGA AAGAAGATCA AGATCAAAGG GAAGATGCGC 
AAACTGGACC GACCTCCAGA GAATCCTGTC GTGGATCCCA CCATTAAGTT TGAGCGTCAG 
CCGGATTATC TGGACACCAC AGGCGGAACG CTGCACCCAT ATCAGCTTGA AGGGCTCAAC 
TGGCTGCGCT TCTCTTGGGC TCAGGGAACC GACACCATCC TAGCAGACGA GATGGGGCTG 
GGAAAGACTG TGCAGACTGC TGTGTTCCTC TATTCACTGT ATAAAGAGGG TCATTCTAAA 
GGCCCGTTCC TGGTCAGTGC ACCGCTGTCC ACCATCATCA ACTGGGAGAG AGAGTTTGAG 
ATGTGGGCTC CGGACATGTA CGTAGTCACA TATGTGGGCG ACAAAGACAG CAGGGCCGTC 
ATCAGAGAAA ACGAGTTCTC CTTCGAAAAC AACGCCATCC GTGGTGGCAA GAAACCCTCC 
AAGATGAAGA AAGAAGCCTC CGTCAAGTTT CACGTGCTGT TGACCTCCTA TGAGTTGATC 
ACTATTGACA CAGCCGTTCT GGGCTCCATC GACTGGGCCT GTCTGGTTGT TGATGAGGCC 
CACAGACTGA AGAATAACCA GTCGAAGTTT TTCAGGATAC TGAACAACTA CCCGCTGCAG 
CATAAGCTGT TGTTGACAGG AACACCACTG CAAAACAACC TAGAAGAGCT TTTCCATCTG 
CTCAACTTCC TCACGCCGGA GAGATTCAGT AATCTTGAGG GTTTCTTGGA GGAGTTTGCG 
GACATCGCCA AGGAAGACCA GATCAAAAAG CTTCATGACA TGCTGGGACC ACACATGCTC 
AGGAGACTCA AAGCCGACGT GTTCAAGCAC ATGCCTTCAA AAACAGAGCT GATTGTGCGC 
GTGGAGCTCA GCCCCATGCA GAAGAAATAT TACAAGTTCA TCCTAACCCG AAACTTCGAG 
GCTCTGAACA CTCGTGGTGG AGGAAATCAG GTTTCTCTGC TGAATGTGGT CATGGACTTG 
AAGAAGTGCT GTAACCATCC TTATCTATTC CCTGCTGCTG CTATGGAAGC TGCCAAAATG 
CCCAATGGGA TGTATGAGGG TGGTGGCCTG ACTAAATCTT CTGGAAAACT GCTGCTACTG 
CAGAAGATGC TGCGCAAGCT GAAGGAGGGT GGACACAGGG TGCTCATCTT CTCTCAGATG 
ACCAAAATGT TGGACCTGCT GGAGGATTTC CTGGAGAATG AGGGCTACAA ATATGAACGT 
ATTGATGGAG GAATCACAGG AGGCATGAGG CAGGAAGCCA TTGACCGCTT TAACGCTCCT 
GGTGCTCCTC AGTTTGTTTT CCTGCTCTCG ACCAGAGCTG GTGGTTTGGG TATCAATCTG 
GCAACCGCAG ACACCGTCGT CATCTACGAT TCAGACTGGA ACCCTCACAA TGACATTCAG 
GCCTTCAGCA GAGCTCACAG GATTGGTCAA AACAAGAAGG TGATGATCTA CCGCTTTGTA 
ACCAAAGCTT CTGTAGAAGA GCGAATTACT CAGGTGGCCA AGAAGAAGAT GATGTTGACT 
CACTTGGTTG TGCGTCCTGG ATTGGGCTCA AAGACTGGTT CGATGTCTAA ACAGGAGCTC 
GATGACATTC TTAAATTCGG TACAGAGGAG CTATTTAAGG ACGAGGGAGA AGGAGAGAAC 
AAAGAAGGAC AAGAGGACAG CAGTGTGATT CACTATGATG ACAAAGCCAT CGATCGCCTG 
CTGGATCGTA ATCAGGATGC GACCGACGAC ACGGAGCTCC AGAGCATGAA CGAGTATCTC 
AGCTCCTTTA AAGTGGCGCA GTATGTGGAG GAGGAAGTTC AGAGGGAAAT CATCAAGCAG 
GAGGAAAGTG TGGATCCAGA TTACTGGGAG AAACTCCTCC GACACCATTA TGAGCAGCAG 
CAGGAGGATT TGGCTCGTAA TCTGGGCAAG GGCAAGCGAA TCCGCAAACA GGTCAACTAC 
AACGACGGCT CTCAGGAGGA CAGAGATTGG CAGGACGATC AATCTGACGG CCAATCAGAC 
TATTCGGTTG CCTCAGAGGA AGGAGACGAG GACTTTGACG AGCGATCAGA AGCCAATTCA 
CGGCGACCCA ACAGGAAAGG CCTCAGAAAC GACAAAGACA AACCACTGCC CCCCCTGCTG 
GCCAGAGTGA GCGGCAACAT AGAGGTGTTG GGTTTCAATG CTCGTCAAAG GAAGGCTTTC 
CTGAATGCTG TGATGCGTTA TGGCATGCCA CCTCAGGACG CCTTCACGAC ACAGTGGCTG 
GTCAGAGACC TGCGAGGCAA ATCAGAGAGA GAGTTTAAGG CTTATGTCTC TCTCTTTATG 
CGGCACTTGT GTGAACCTGG CGCAGATGGT GCTGAAAGCT TTGCAGATGG TGTTCCTCGA 
GAGGGACTTT CACGGCAGCA CGTCCTCACG CGCATCGGTG TGATGTCGCT CATCCGAAAG 
AAGGTGCAAG AGTTTGAGCA TGTGAACGGT CAGTGGTCGA TGCCCTGGAT GATGGAGCTG 
AGCGAGAATA AGACCACGTC CACCGCCGGC AGCCATCCAG ACTCTCCCAG CAAAACCCCC 
ACTACTGGCA CTCCATCAGA CACCCAGCCT AACACACCTG CTCCAGACGG TGTTCCCAAA 
GCAGAAGATG CTGGTAAAGA TGCTGATAAA GAAAAGGTGA AAAATGGAGA CGGAGAGAAG 
GAGGCCAATG AAAACAAAAA CAGTGAAGTC ATTGCTGTTC CGGATGAAGA TGGCCGAGCT 
GCTTCTGCGT CTGAGGAGAA AGAGAAAGGC AAAGAGGAGA AGGAGAAGAC GCCTGAATCC 
TCCTCGGAGG CAGAAAAGGC TGATGGGAAG CAGGAAGAGA AGGAAGAAGA AAACGCCAAG 
TCGGGAGACT CTGAGGAGAA AACTGATGAT ACAGACAAAC CCAAAGGATC TGAGGCCGAC 
AAGGAGACTT CAGACGCCAA AGGAGAAAAG AAGGATGATG AGCCTGAGAA GATGGACACA 
ACTCCTGTTA CAGATGAGAA GAAAGGTCAG AAGGATGAGA AAGATTCTGG TAAAACAGAA 
GAAGCGGGAA AACTGCCTAA TGGAGAAAGT GCCAAAGACA GCGCAGCGGG AGCAGGAATT 
GAGGAGAGGA AGAAAGCCAA AACACGGTTC ATGTTCAACA TTGCAGATGG AGGATTCACC 
GAATTGCACT CCCTGTGGCA GAATGAGGAA CGAGCTGCTA CAGTCACTAA GAAAACCAAT 
GAGATCTGGC ATCGTCGTCA TGACTACTGG CTCCTCGCAG GCATCATCCA GTATCTTTTA 
ATGCAACACG GGTACGCTCG CTGGCAAGAC ATTCAGAATG ACGTCCGGTT CGCCATTCTT 
AACGAACCCT TCAAAGGAGA GATCAACAGA GGAAACTTCC TGGAGATCAA AAACAAGTTT 
CTTGCCCGGA GGTTCAAGTT GCTCGAGCAG GCTCTGGTGA TTGAAGAACA GCTGCGCAGA 
GCTGCGTATC TGAACATGAC TGAAGACCCG TCTCACCCCT CCATGGCTCT CAACACACGC 
TTCAGTGAAG TGGAGTGTCT GGCCGAGTCC CACCAGCACC TGAGCAAAGA GTCCATGTCT 
GGAAACAAAC CTGCCAACGC CGTCCTACAT AAAGTACTGA AGCAGCTGGA GGAGTTGCTA 
AGTGACATGA AAGCCGATGT CACTCGTCTC CCGGCAACCA TCGCCAGGAT ACCGCCTGTT 
GCAGTGAGGC TGCAGATGTC TGAAAGAAAC ATTCTCAGCC GTTTGGCCAG CAGAGGACCA 
GACACACAGA GTCAGCAGGT GCAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd4a ( XM_005159352.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159409.1 Danio rerio chromodomain helicase DNA binding protein 4a (chd4a), transcript variant X2, mRNA.

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CloneID	ODa23814
Clone ID Related Accession (Same CDS sequence)	XM_005159352.3
Accession Version	XM_005159352.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain helicase DNA binding protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCA GTGAGGACGA TAGAGACGAT TTTGAACATC GGTCTGTGAT GCAAGAAGAG 
GACGACCTCG AAGAGGAGTT GTCAGAGAAT GATGCCCCAA AGGTGAAAAA GAAGAAGAAA 
GCCAAAAAAA GTAGCCGCGA GAGCAAAGGC AGCAAGAGAA ATCGCTCCCG CAGAGAGGAA 
ATTGGCATCA GTTCTCCAGA ACCAGGTGAA GGCCCAGATC TGGACGATGG TGAGGAGGAC 
CGCTCGGAAA GTGAAGGTAG TGACTACACA CCAGGCAGAA AAAAGAAGAA GAGAGCCAGC 
ACAGCGAAGG ATAAGAAGCG AAACAGTTCT GCCGCAGAGA GAAACTCTGC TGCCTCCAAA 
CGGAGGGAAC CGGAGGAAGA GGAAGACGAG GATGATGACG ATTCTGAACC AAAAAGTTCC 
TCTCAGCTTT TGGATACCTG GGGAATGGAG GACATCGACC ATATTTTCAC CGAGGAAGAT 
TATAGGACTC TGACCAACTA TAAAGCCTTC AGCCAGTTTG TCAGGCCTCT GATCGCAGCC 
AAGAACCCCA AAATTGCGGT TTCCAAGATG ATGATGGTTC TGGGAGCGAA GTGGCGGGAG 
TTCAGCACTA ACAATCCTCT GCGGGGCTCT GCTACATCTC TGGTGGCCGC TGCCTCTGTC 
GAAACGCCAC CGGAAACTCC AACCTCTGCA TCCGCAGCTC AGATCAGCCC CGAGTCCACG 
CCAGCAGCTC CCGTTCGCAA GGCCAAGACA AAAGAGGGCA AGGGTCCCAA TGCACGCAAG 
AAGTCCAAAC CAGCTCCTAA ACCCCAGGAG AAGAAGGTCA AAACCAAAAA GGTGGCTCCC 
TTGAAGATCA AACTAGGAGG CTTCAACAGC AAGAGGAAAC GCTCATCTAG TGAAGAGGAT 
GATGTTGATG TGGACAGTGA CTTTGACGAC GGCAGTATTA ACAGCGTGTC TGTTTCAAAT 
GGATCGAATA GTCGTAGCAG TCGCAGCAGC GCCAAAAAGA AGCCCAAAAA GAAGACGAAA 
AAGGATGAAG ATGGTGATGG CTATGAGACA GATCATCAGG ATTACTGTGA GGTCTGCCAG 
CAGGGAGGTG AGATCATTCT CTGTGACACC TGTCCACGGG CCTATCACAT GGTCTGCTTG 
GACCCCGACA TGGAGAAGGC CCCTGAGGGT ACCTGGAGCT GCCCACACTG CGAGAAGATG 
GGCATCCAGT GGGAGGCTCG TGAAGACGCG TCTGAGGGCG AGGAGGATAA TGAGGCAGGA 
GGAGAGGCCG AAGAAGACGA CCATCACATG GAGTTCTGCA GAGTCTGTAA GGATGGCGGA 
GAGCTGCTGT GCTGCGACTC ATGCCCTTCG TCATACCACA TCCACTGCCT GAACCCACCG 
CTGCCCGAGA TCCCCAACGG AGAGTGGATC TGCCCTCGCT GCACTTGTCC CTCTATGAAA 
GGTAAAGTGC AGAAGATTCT GACGTGGCGC TGGACTGAAC CTCCACCGCC GACCCCTGTC 
CCACGTCCCT CTGACCTGCC AACTGATGCT CCTGACCCCA CACCTCTGGC TGGACGTCCT 
GAGAGAGAGT TCTTCGTTAA ATGGCAGAAT ATGTCCTACT GGCACTGCTC CTGGGTTACT 
GAGCTGCAGC TGGAGATTCA CTGTCAGGTG ATGTTCAGAA ACTACCAGCG CAAGAACGAC 
ATGGACGAAC CTCCCCCCAT AGACTTCGGA GGTGAAGGAG AAGAGGAGAA GAGCGACAAG 
AGAAAGAAAA AAGACCCCAC ATACGCACGG ATGGAGGAGA AGTACTACCG CTTCGGCATC 
AAGATGGAGT GGATGGTCAT TCATCGAATC CTGAACCACA GTGTGGATAA GAAGAACAAC 
TGTCACTACC TCATCAAATG GAGGGACTTA ACATATGATC AAGCCACATG GGAGTTGGAG 
GATATGGATT TACCAGACTA TGACACGTAT AAACTGCAGT ACTGGAACCA CAGGGAGCTG 
ATGATGGGTG ATGAAGGTAA ACCAGGAAAG AAGATCAAGA TCAAAGGGAA GATGCGCAAA 
CTGGACCGAC CTCCAGAGAA TCCTGTCGTG GATCCCACCA TTAAGTTTGA GCGTCAGCCG 
GATTATCTGG ACACCACAGG CGGAACGCTG CACCCATATC AGCTTGAAGG GCTCAACTGG 
CTGCGCTTCT CTTGGGCTCA GGGAACCGAC ACCATCCTAG CAGACGAGAT GGGGCTGGGA 
AAGACTGTGC AGACTGCTGT GTTCCTCTAT TCACTGTATA AAGAGGGTCA TTCTAAAGGC 
CCGTTCCTGG TCAGTGCACC GCTGTCCACC ATCATCAACT GGGAGAGAGA GTTTGAGATG 
TGGGCTCCGG ACATGTACGT AGTCACATAT GTGGGCGACA AAGACAGCAG GGCCGTCATC 
AGAGAAAACG AGTTCTCCTT CGAAAACAAC GCCATCCGTG GTGGCAAGAA ACCCTCCAAG 
ATGAAGAAAG AAGCCTCCGT CAAGTTTCAC GTGCTGTTGA CCTCCTATGA GTTGATCACT 
ATTGACACAG CCGTTCTGGG CTCCATCGAC TGGGCCTGTC TGGTTGTTGA TGAGGCCCAC 
AGACTGAAGA ATAACCAGTC GAAGTTTTTC AGGATACTGA ACAACTACCC GCTGCAGCAT 
AAGCTGTTGT TGACAGGAAC ACCACTGCAA AACAACCTAG AAGAGCTTTT CCATCTGCTC 
AACTTCCTCA CGCCGGAGAG ATTCAGTAAT CTTGAGGGTT TCTTGGAGGA GTTTGCGGAC 
ATCGCCAAGG AAGACCAGAT CAAAAAGCTT CATGACATGC TGGGACCACA CATGCTCAGG 
AGACTCAAAG CCGACGTGTT CAAGCACATG CCTTCAAAAA CAGAGCTGAT TGTGCGCGTG 
GAGCTCAGCC CCATGCAGAA GAAATATTAC AAGTTCATCC TAACCCGAAA CTTCGAGGCT 
CTGAACACTC GTGGTGGAGG AAATCAGGTT TCTCTGCTGA ATGTGGTCAT GGACTTGAAG 
AAGTGCTGTA ACCATCCTTA TCTATTCCCT GCTGCTGCTA TGGAAGCTGC CAAAATGCCC 
AATGGGATGT ATGAGGGTGG TGGCCTGACT AAATCTTCTG GAAAACTGCT GCTACTGCAG 
AAGATGCTGC GCAAGCTGAA GGAGGGTGGA CACAGGGTGC TCATCTTCTC TCAGATGACC 
AAAATGTTGG ACCTGCTGGA GGATTTCCTG GAGAATGAGG GCTACAAATA TGAACGTATT 
GATGGAGGAA TCACAGGAGG CATGAGGCAG GAAGCCATTG ACCGCTTTAA CGCTCCTGGT 
GCTCCTCAGT TTGTTTTCCT GCTCTCGACC AGAGCTGGTG GTTTGGGTAT CAATCTGGCA 
ACCGCAGACA CCGTCGTCAT CTACGATTCA GACTGGAACC CTCACAATGA CATTCAGGCC 
TTCAGCAGAG CTCACAGGAT TGGTCAAAAC AAGAAGGTGA TGATCTACCG CTTTGTAACC 
AAAGCTTCTG TAGAAGAGCG AATTACTCAG GTGGCCAAGA AGAAGATGAT GTTGACTCAC 
TTGGTTGTGC GTCCTGGATT GGGCTCAAAG ACTGGTTCGA TGTCTAAACA GGAGCTCGAT 
GACATTCTTA AATTCGGTAC AGAGGAGCTA TTTAAGGACG AGGGAGAAGG AGAGAACAAA 
GAAGGACAAG AGGACAGCAG TGTGATTCAC TATGATGACA AAGCCATCGA TCGCCTGCTG 
GATCGTAATC AGGATGCGAC CGACGACACG GAGCTCCAGA GCATGAACGA GTATCTCAGC 
TCCTTTAAAG TGGCGCAGTA TGTGGTCAAG GATGAGGAAG AGGCGGAGGA GGAAGTTCAG 
AGGGAAATCA TCAAGCAGGA GGAAAGTGTG GATCCAGATT ACTGGGAGAA ACTCCTCCGA 
CACCATTATG AGCAGCAGCA GGAGGATTTG GCTCGTAATC TGGGCAAGGG CAAGCGAATC 
CGCAAACAGG TCAACTACAA CGACGGCTCT CAGGAGGACA GAGATTGGCA GGACGATCAA 
TCTGACGGCC AATCAGACTA TTCGGTTGCC TCAGAGGAAG GAGACGAGGA CTTTGACGAG 
CGATCAGAAG CCAATTCACG GCGACCCAAC AGGAAAGGCC TCAGAAACGA CAAAGACAAA 
CCACTGCCCC CCCTGCTGGC CAGAGTGAGC GGCAACATAG AGGTGTTGGG TTTCAATGCT 
CGTCAAAGGA AGGCTTTCCT GAATGCTGTG ATGCGTTATG GCATGCCACC TCAGGACGCC 
TTCACGACAC AGTGGCTGGT CAGAGACCTG CGAGGCAAAT CAGAGAGAGA GTTTAAGGCT 
TATGTCTCTC TCTTTATGCG GCACTTGTGT GAACCTGGCG CAGATGGTGC TGAAAGCTTT 
GCAGATGGTG TTCCTCGAGA GGGACTTTCA CGGCAGCACG TCCTCACGCG CATCGGTGTG 
ATGTCGCTCA TCCGAAAGAA GGTGCAAGAG TTTGAGCATG TGAACGGTCA GTGGTCGATG 
CCCTGGATGA TGGAGCTGAG CGAGAATAAG ACCACGTCCA CCGCCGGCAG CCATCCAGAC 
TCTCCCAGCA AAACCCCCAC TACTGGCACT CCATCAGACA CCCAGCCTAA CACACCTGCT 
CCAGACGGTG TTCCCAAAGC AGAAGATGCT GGTAAAGATG CTGATAAAGA AAAGGTGAAA 
AATGGAGACG GAGAGAAGGA GGCCAATGAA AACAAAAACA GTGAAGTCAT TGCTGTTCCG 
GATGAAGATG GCCGAGCTGC TTCTGCGTCT GAGGAGAAAG AGAAAGGCAA AGAGGAGAAG 
GAGAAGACGC CTGAATCCTC CTCGGAGGCA GAAAAGGCTG ATGGGAAGCA GGAAGAGAAG 
GAAGAAGAAA ACGCCAAGTC GGGAGACTCT GAGGAGAAAA CTGATGATAC AGACAAACCC 
AAAGGATCTG AGGCCGACAA GGAGACTTCA GACGCCAAAG GAGAAAAGAA GGATGATGAG 
CCTGAGAAGA TGGACACAAC TCCTGTTACA GATGAGAAGA AAGGTCAGAA GGATGAGAAA 
GATTCTGGTA AAACAGAAGA AGCGGGAAAA CTGCCTAATG GAGAAAGTGC CAAAGACAGC 
GCAGCGGGAG CAGGAATTGA GGAGAGGAAG AAAGCCAAAA CACGGTTCAT GTTCAACATT 
GCAGATGGAG GATTCACCGA ATTGCACTCC CTGTGGCAGA ATGAGGAACG AGCTGCTACA 
GTCACTAAGA AAACCAATGA GATCTGGCAT CGTCGTCATG ACTACTGGCT CCTCGCAGGC 
ATCATCCAAC ACGGGTACGC TCGCTGGCAA GACATTCAGA ATGACGTCCG GTTCGCCATT 
CTTAACGAAC CCTTCAAAGG AGAGATCAAC AGAGGAAACT TCCTGGAGAT CAAAAACAAG 
TTTCTTGCCC GGAGGTTCAA GTTGCTCGAG CAGGCTCTGG TGATTGAAGA ACAGCTGCGC 
AGAGCTGCGT ATCTGAACAT GACTGAAGAC CCGTCTCACC CCTCCATGGC TCTCAACACA 
CGCTTCAGTG AAGTGGAGTG TCTGGCCGAG TCCCACCAGC ACCTGAGCAA AGAGTCCATG 
TCTGGAAACA AACCTGCCAA CGCCGTCCTA CATAAAGTAC TGAAGCAGCT GGAGGAGTTG 
CTAAGTGACA TGAAAGCCGA TGTCACTCGT CTCCCGGCAA CCATCGCCAG GATACCGCCT 
GTTGCAGTGA GGCTGCAGAT GTCTGAAAGA AACATTCTCA GCCGTTTGGC CAGCAGAGGA 
CCAGACACAC AGAGTCAGCA GGTGCAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159409.1
CDS	347..6139
Translation	MSGSEDDRDDFEHRSVMQEEDDLEEELSENDAPKVKKKKKAKKSSRESKGSKRNRSRREEIGISSPEPGEGPDLDDGEEDRSESEGSDYTPGRKKKKRASTAKDKKRNSSAAERNSAASKRREPEEEEDEDDDDSEPKSSSQLLDTWGMEDIDHIFTEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPLRGSATSLVAAASVETPPETPTSASAAQISPESTPAAPVRKAKTKEGKGPNARKKSKPAPKPQEKKVKTKKVAPLKIKLGGFNSKRKRSSSEEDDVDVDSDFDDGSINSVSVSNGSNSRSSRSSAKKKPKKKTKKDEDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGTWSCPHCEKMGIQWEAREDASEGEEDNEAGGEAEEDDHHMEFCRVCKDGGELLCCDSCPSSYHIHCLNPPLPEIPNGEWICPRCTCPSMKGKVQKILTWRWTEPPPPTPVPRPSDLPTDAPDPTPLAGRPEREFFVKWQNMSYWHCSWVTELQLEIHCQVMFRNYQRKNDMDEPPPIDFGGEGEEEKSDKRKKKDPTYARMEEKYYRFGIKMEWMVIHRILNHSVDKKNNCHYLIKWRDLTYDQATWELEDMDLPDYDTYKLQYWNHRELMMGDEGKPGKKIKIKGKMRKLDRPPENPVVDPTIKFERQPDYLDTTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAVIRENEFSFENNAIRGGKKPSKMKKEASVKFHVLLTSYELITIDTAVLGSIDWACLVVDEAHRLKNNQSKFFRILNNYPLQHKLLLTGTPLQNNLEELFHLLNFLTPERFSNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKHMPSKTELIVRVELSPMQKKYYKFILTRNFEALNTRGGGNQVSLLNVVMDLKKCCNHPYLFPAAAMEAAKMPNGMYEGGGLTKSSGKLLLLQKMLRKLKEGGHRVLIFSQMTKMLDLLEDFLENEGYKYERIDGGITGGMRQEAIDRFNAPGAPQFVFLLSTRAGGLGINLATADTVVIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTKASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEGEGENKEGQEDSSVIHYDDKAIDRLLDRNQDATDDTELQSMNEYLSSFKVAQYVVKDEEEAEEEVQREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDGQSDYSVASEEGDEDFDERSEANSRRPNRKGLRNDKDKPLPPLLARVSGNIEVLGFNARQRKAFLNAVMRYGMPPQDAFTTQWLVRDLRGKSEREFKAYVSLFMRHLCEPGADGAESFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGQWSMPWMMELSENKTTSTAGSHPDSPSKTPTTGTPSDTQPNTPAPDGVPKAEDAGKDADKEKVKNGDGEKEANENKNSEVIAVPDEDGRAASASEEKEKGKEEKEKTPESSSEAEKADGKQEEKEEENAKSGDSEEKTDDTDKPKGSEADKETSDAKGEKKDDEPEKMDTTPVTDEKKGQKDEKDSGKTEEAGKLPNGESAKDSAAGAGIEERKKAKTRFMFNIADGGFTELHSLWQNEERAATVTKKTNEIWHRRHDYWLLAGIIQHGYARWQDIQNDVRFAILNEPFKGEINRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMTEDPSHPSMALNTRFSEVECLAESHQHLSKESMSGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLASRGPDTQSQQVQQ

Target ORF information:

RefSeq Version	XM_005159352.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 4a (chd4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159352.3

ORF Insert Sequence:

ATGTCCGGCA GTGAGGACGA TAGAGACGAT TTTGAACATC GGTCTGTGAT GCAAGAAGAG 
GACGACCTCG AAGAGGAGTT GTCAGAGAAT GATGCCCCAA AGGTGAAAAA GAAGAAGAAA 
GCCAAAAAAA GTAGCCGCGA GAGCAAAGGC AGCAAGAGAA ATCGCTCCCG CAGAGAGGAA 
ATTGGCATCA GTTCTCCAGA ACCAGGTGAA GGCCCAGATC TGGACGATGG TGAGGAGGAC 
CGCTCGGAAA GTGAAGGTAG TGACTACACA CCAGGCAGAA AAAAGAAGAA GAGAGCCAGC 
ACAGCGAAGG ATAAGAAGCG AAACAGTTCT GCCGCAGAGA GAAACTCTGC TGCCTCCAAA 
CGGAGGGAAC CGGAGGAAGA GGAAGACGAG GATGATGACG ATTCTGAACC AAAAAGTTCC 
TCTCAGCTTT TGGATACCTG GGGAATGGAG GACATCGACC ATATTTTCAC CGAGGAAGAT 
TATAGGACTC TGACCAACTA TAAAGCCTTC AGCCAGTTTG TCAGGCCTCT GATCGCAGCC 
AAGAACCCCA AAATTGCGGT TTCCAAGATG ATGATGGTTC TGGGAGCGAA GTGGCGGGAG 
TTCAGCACTA ACAATCCTCT GCGGGGCTCT GCTACATCTC TGGTGGCCGC TGCCTCTGTC 
GAAACGCCAC CGGAAACTCC AACCTCTGCA TCCGCAGCTC AGATCAGCCC CGAGTCCACG 
CCAGCAGCTC CCGTTCGCAA GGCCAAGACA AAAGAGGGCA AGGGTCCCAA TGCACGCAAG 
AAGTCCAAAC CAGCTCCTAA ACCCCAGGAG AAGAAGGTCA AAACCAAAAA GGTGGCTCCC 
TTGAAGATCA AACTAGGAGG CTTCAACAGC AAGAGGAAAC GCTCATCTAG TGAAGAGGAT 
GATGTTGATG TGGACAGTGA CTTTGACGAC GGCAGTATTA ACAGCGTGTC TGTTTCAAAT 
GGATCGAATA GTCGTAGCAG TCGCAGCAGC GCCAAAAAGA AGCCCAAAAA GAAGACGAAA 
AAGGATGAAG ATGGTGATGG CTATGAGACA GATCATCAGG ATTACTGTGA GGTCTGCCAG 
CAGGGAGGTG AGATCATTCT CTGTGACACC TGTCCACGGG CCTATCACAT GGTCTGCTTG 
GACCCCGACA TGGAGAAGGC CCCTGAGGGT ACCTGGAGCT GCCCACACTG CGAGAAGATG 
GGCATCCAGT GGGAGGCTCG TGAAGACGCG TCTGAGGGCG AGGAGGATAA TGAGGCAGGA 
GGAGAGGCCG AAGAAGACGA CCATCACATG GAGTTCTGCA GAGTCTGTAA GGATGGCGGA 
GAGCTGCTGT GCTGCGACTC ATGCCCTTCG TCATACCACA TCCACTGCCT GAACCCACCG 
CTGCCCGAGA TCCCCAACGG AGAGTGGATC TGCCCTCGCT GCACTTGTCC CTCTATGAAA 
GGTAAAGTGC AGAAGATTCT GACGTGGCGC TGGACTGAAC CTCCACCGCC GACCCCTGTC 
CCACGTCCCT CTGACCTGCC AACTGATGCT CCTGACCCCA CACCTCTGGC TGGACGTCCT 
GAGAGAGAGT TCTTCGTTAA ATGGCAGAAT ATGTCCTACT GGCACTGCTC CTGGGTTACT 
GAGCTGCAGC TGGAGATTCA CTGTCAGGTG ATGTTCAGAA ACTACCAGCG CAAGAACGAC 
ATGGACGAAC CTCCCCCCAT AGACTTCGGA GGTGAAGGAG AAGAGGAGAA GAGCGACAAG 
AGAAAGAAAA AAGACCCCAC ATACGCACGG ATGGAGGAGA AGTACTACCG CTTCGGCATC 
AAGATGGAGT GGATGGTCAT TCATCGAATC CTGAACCACA GTGTGGATAA GAAGAACAAC 
TGTCACTACC TCATCAAATG GAGGGACTTA ACATATGATC AAGCCACATG GGAGTTGGAG 
GATATGGATT TACCAGACTA TGACACGTAT AAACTGCAGT ACTGGAACCA CAGGGAGCTG 
ATGATGGGTG ATGAAGGTAA ACCAGGAAAG AAGATCAAGA TCAAAGGGAA GATGCGCAAA 
CTGGACCGAC CTCCAGAGAA TCCTGTCGTG GATCCCACCA TTAAGTTTGA GCGTCAGCCG 
GATTATCTGG ACACCACAGG CGGAACGCTG CACCCATATC AGCTTGAAGG GCTCAACTGG 
CTGCGCTTCT CTTGGGCTCA GGGAACCGAC ACCATCCTAG CAGACGAGAT GGGGCTGGGA 
AAGACTGTGC AGACTGCTGT GTTCCTCTAT TCACTGTATA AAGAGGGTCA TTCTAAAGGC 
CCGTTCCTGG TCAGTGCACC GCTGTCCACC ATCATCAACT GGGAGAGAGA GTTTGAGATG 
TGGGCTCCGG ACATGTACGT AGTCACATAT GTGGGCGACA AAGACAGCAG GGCCGTCATC 
AGAGAAAACG AGTTCTCCTT CGAAAACAAC GCCATCCGTG GTGGCAAGAA ACCCTCCAAG 
ATGAAGAAAG AAGCCTCCGT CAAGTTTCAC GTGCTGTTGA CCTCCTATGA GTTGATCACT 
ATTGACACAG CCGTTCTGGG CTCCATCGAC TGGGCCTGTC TGGTTGTTGA TGAGGCCCAC 
AGACTGAAGA ATAACCAGTC GAAGTTTTTC AGGATACTGA ACAACTACCC GCTGCAGCAT 
AAGCTGTTGT TGACAGGAAC ACCACTGCAA AACAACCTAG AAGAGCTTTT CCATCTGCTC 
AACTTCCTCA CGCCGGAGAG ATTCAGTAAT CTTGAGGGTT TCTTGGAGGA GTTTGCGGAC 
ATCGCCAAGG AAGACCAGAT CAAAAAGCTT CATGACATGC TGGGACCACA CATGCTCAGG 
AGACTCAAAG CCGACGTGTT CAAGCACATG CCTTCAAAAA CAGAGCTGAT TGTGCGCGTG 
GAGCTCAGCC CCATGCAGAA GAAATATTAC AAGTTCATCC TAACCCGAAA CTTCGAGGCT 
CTGAACACTC GTGGTGGAGG AAATCAGGTT TCTCTGCTGA ATGTGGTCAT GGACTTGAAG 
AAGTGCTGTA ACCATCCTTA TCTATTCCCT GCTGCTGCTA TGGAAGCTGC CAAAATGCCC 
AATGGGATGT ATGAGGGTGG TGGCCTGACT AAATCTTCTG GAAAACTGCT GCTACTGCAG 
AAGATGCTGC GCAAGCTGAA GGAGGGTGGA CACAGGGTGC TCATCTTCTC TCAGATGACC 
AAAATGTTGG ACCTGCTGGA GGATTTCCTG GAGAATGAGG GCTACAAATA TGAACGTATT 
GATGGAGGAA TCACAGGAGG CATGAGGCAG GAAGCCATTG ACCGCTTTAA CGCTCCTGGT 
GCTCCTCAGT TTGTTTTCCT GCTCTCGACC AGAGCTGGTG GTTTGGGTAT CAATCTGGCA 
ACCGCAGACA CCGTCGTCAT CTACGATTCA GACTGGAACC CTCACAATGA CATTCAGGCC 
TTCAGCAGAG CTCACAGGAT TGGTCAAAAC AAGAAGGTGA TGATCTACCG CTTTGTAACC 
AAAGCTTCTG TAGAAGAGCG AATTACTCAG GTGGCCAAGA AGAAGATGAT GTTGACTCAC 
TTGGTTGTGC GTCCTGGATT GGGCTCAAAG ACTGGTTCGA TGTCTAAACA GGAGCTCGAT 
GACATTCTTA AATTCGGTAC AGAGGAGCTA TTTAAGGACG AGGGAGAAGG AGAGAACAAA 
GAAGGACAAG AGGACAGCAG TGTGATTCAC TATGATGACA AAGCCATCGA TCGCCTGCTG 
GATCGTAATC AGGATGCGAC CGACGACACG GAGCTCCAGA GCATGAACGA GTATCTCAGC 
TCCTTTAAAG TGGCGCAGTA TGTGGTCAAG GATGAGGAAG AGGCGGAGGA GGAAGTTCAG 
AGGGAAATCA TCAAGCAGGA GGAAAGTGTG GATCCAGATT ACTGGGAGAA ACTCCTCCGA 
CACCATTATG AGCAGCAGCA GGAGGATTTG GCTCGTAATC TGGGCAAGGG CAAGCGAATC 
CGCAAACAGG TCAACTACAA CGACGGCTCT CAGGAGGACA GAGATTGGCA GGACGATCAA 
TCTGACGGCC AATCAGACTA TTCGGTTGCC TCAGAGGAAG GAGACGAGGA CTTTGACGAG 
CGATCAGAAG CCAATTCACG GCGACCCAAC AGGAAAGGCC TCAGAAACGA CAAAGACAAA 
CCACTGCCCC CCCTGCTGGC CAGAGTGAGC GGCAACATAG AGGTGTTGGG TTTCAATGCT 
CGTCAAAGGA AGGCTTTCCT GAATGCTGTG ATGCGTTATG GCATGCCACC TCAGGACGCC 
TTCACGACAC AGTGGCTGGT CAGAGACCTG CGAGGCAAAT CAGAGAGAGA GTTTAAGGCT 
TATGTCTCTC TCTTTATGCG GCACTTGTGT GAACCTGGCG CAGATGGTGC TGAAAGCTTT 
GCAGATGGTG TTCCTCGAGA GGGACTTTCA CGGCAGCACG TCCTCACGCG CATCGGTGTG 
ATGTCGCTCA TCCGAAAGAA GGTGCAAGAG TTTGAGCATG TGAACGGTCA GTGGTCGATG 
CCCTGGATGA TGGAGCTGAG CGAGAATAAG ACCACGTCCA CCGCCGGCAG CCATCCAGAC 
TCTCCCAGCA AAACCCCCAC TACTGGCACT CCATCAGACA CCCAGCCTAA CACACCTGCT 
CCAGACGGTG TTCCCAAAGC AGAAGATGCT GGTAAAGATG CTGATAAAGA AAAGGTGAAA 
AATGGAGACG GAGAGAAGGA GGCCAATGAA AACAAAAACA GTGAAGTCAT TGCTGTTCCG 
GATGAAGATG GCCGAGCTGC TTCTGCGTCT GAGGAGAAAG AGAAAGGCAA AGAGGAGAAG 
GAGAAGACGC CTGAATCCTC CTCGGAGGCA GAAAAGGCTG ATGGGAAGCA GGAAGAGAAG 
GAAGAAGAAA ACGCCAAGTC GGGAGACTCT GAGGAGAAAA CTGATGATAC AGACAAACCC 
AAAGGATCTG AGGCCGACAA GGAGACTTCA GACGCCAAAG GAGAAAAGAA GGATGATGAG 
CCTGAGAAGA TGGACACAAC TCCTGTTACA GATGAGAAGA AAGGTCAGAA GGATGAGAAA 
GATTCTGGTA AAACAGAAGA AGCGGGAAAA CTGCCTAATG GAGAAAGTGC CAAAGACAGC 
GCAGCGGGAG CAGGAATTGA GGAGAGGAAG AAAGCCAAAA CACGGTTCAT GTTCAACATT 
GCAGATGGAG GATTCACCGA ATTGCACTCC CTGTGGCAGA ATGAGGAACG AGCTGCTACA 
GTCACTAAGA AAACCAATGA GATCTGGCAT CGTCGTCATG ACTACTGGCT CCTCGCAGGC 
ATCATCCAAC ACGGGTACGC TCGCTGGCAA GACATTCAGA ATGACGTCCG GTTCGCCATT 
CTTAACGAAC CCTTCAAAGG AGAGATCAAC AGAGGAAACT TCCTGGAGAT CAAAAACAAG 
TTTCTTGCCC GGAGGTTCAA GTTGCTCGAG CAGGCTCTGG TGATTGAAGA ACAGCTGCGC 
AGAGCTGCGT ATCTGAACAT GACTGAAGAC CCGTCTCACC CCTCCATGGC TCTCAACACA 
CGCTTCAGTG AAGTGGAGTG TCTGGCCGAG TCCCACCAGC ACCTGAGCAA AGAGTCCATG 
TCTGGAAACA AACCTGCCAA CGCCGTCCTA CATAAAGTAC TGAAGCAGCT GGAGGAGTTG 
CTAAGTGACA TGAAAGCCGA TGTCACTCGT CTCCCGGCAA CCATCGCCAG GATACCGCCT 
GTTGCAGTGA GGCTGCAGAT GTCTGAAAGA AACATTCTCA GCCGTTTGGC CAGCAGAGGA 
CCAGACACAC AGAGTCAGCA GGTGCAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rescue of neural crest-derived phenotypes in a zebrafish CHARGE model by Sox10 downregulation.
Human molecular genetics25(16)3539-3554(2016 08)
Asad Z,Pandey A,Babu A,Sun Y,Shevade K,Kapoor S,Ullah I,Ranjan S,Scaria V,Bajpai R,Sachidanandan C

The chromatin remodeler chd5 is necessary for proper head development during embryogenesis of Danio rerio.
Biochimica et biophysica acta1849(8)1040-50(2015 Aug)
Bishop B,Ho KK,Tyler K,Smith A,Bonilla S,Leung YF,Ogas J

Specific NuRD components are required for fin regeneration in zebrafish.
BMC biology1230(2014 Apr)
Pfefferli C,M?ller F,Ja?wi?ska A,Wicky C

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

Expansion of transducin subunit gene families in early vertebrate tetraploidizations.
Genomics100(4)203-11(2012 Oct)
Lagman D,Sundstr?m G,Ocampo Daza D,Abalo XM,Larhammar D

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chd4a cDNA ORF clone, Danio rerio(zebrafish)

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ORF Insert Sequence:

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