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mlc1 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol mlc1
Entrez Gene ID 559990
Full Name megalencephalic leukoencephalopathy with subcortical cysts 1
Synonyms si:ch211-192n14.1
General protein information
Preferred Names

megalencephalic leukoencephalopathy with subcortical cysts 1

Names

membrane protein MLC1
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_005169941.3 XP_005169998.1 membrane protein MLC1 isoform X1
XM_005169943.3 XP_005170000.1 membrane protein MLC1 isoform X2
XM_017351934.2 XP_017207423.1 membrane protein MLC1 isoform X1
XM_005169941.4 XP_005169998.1 membrane protein MLC1 isoform X1
XM_021467665.1 XP_021323340.1 membrane protein MLC1 isoform X1
XM_005169943.4 XP_005170000.1 membrane protein MLC1 isoform X2
XM_017351934.1 XP_017207423.1 membrane protein MLC1 isoform X1
NM_001104939.1 NP_001098409.1 membrane protein MLC1

Pathways from BioSystems


Homologies


MLC1 XP_001111622.1
Mus musculus (house mouse) Mlc1 NP_573504.1
Rattus norvegicus (Norway rat) Mlc1 NP_001101575.1
Danio rerio (zebrafish) mlc1 NP_001098409.1
Homo sapiens (human) MLC1 NP_631941.1
Gallus gallus (chicken) MLC1 XP_004937559.1
Xenopus tropicalis (tropical clawed frog) mlc1 NP_001072921.1
Canis lupus familiaris (dog) MLC1 XP_848790.2
Pan troglodytes (chimpanzee) MLC1 XP_515212.3
Bos taurus (cattle) MLC1 NP_001070420.1

Gene Ontology


Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Megalencephalic leukoencephalopathy with subcortical cysts protein 1 regulates glial surface localization of GLIALCAM from fish to humans.
Sirisi S, Folgueira M, L?pez-Hern?ndez T, Minieri L, P?rez-Rius C, Gait?n-Pe?as H, Zang J, Mart?nez A, Capdevila-Nortes X, De La Villa P, Roy U, Alia A, Neuhauss S, Ferroni S, Nunes V, Est?vez R, Barrallo-Gimeno A
Human molecular genetics23(19)5069-86(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mlc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mlc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa31747 NM_001104939.1
Latest version!		 Danio rerio modulator of VRAC current 1 (mlc1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
ODa23165 XM_021467665.1
Latest version!		 Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
ODa23165 XM_005169941.4
Latest version!		 Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
ODa23165 XM_017351934.2
Latest version!		 Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
ODa23167 XM_005169943.4
Latest version!		 Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
View Old Accession Versions >>
ODa23165 XM_005169941.3 Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
ODa23167 XM_005169943.3 Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
ODa23165 XM_017351934.1 Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa31747
Clone ID Related Accession (Same CDS sequence) NM_001104939.1
Accession Version NM_001104939.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1176bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-30
Organism Danio rerio(zebrafish)
Product membrane protein MLC1
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151967.1. On Sep 16, 2007 this sequence version replaced XM_683381.1. ##Evidence-Data-START## Transcript exon combination :: BC151967.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

1
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961
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ATGCAGCGAG AGGAGGCATC GGCTCAGGAA GTGTTCAGCT ATGCCCAGAT GTCCACCCTG 
GAGGGAAACA GCGGTGTAGG AACGCTGAGT CGGCATCTAG AGCGCGAGCG ATTGGAGAGA 
GACGGCTACA CGGTGGATGT CAGAGCCAGT GACCTGCAGC TGGATCAGCC TGGCCCGCTA 
CATCCCTGCT TCAGCTACAG AGTCTGGCTT TACAGCGTGG TTGTAGGGAG CAGCTTGCTG 
ATAGCGTCGG CTTTCTCGCT CTACATGGGT AATGTGTTTC CTGCTGCAAT GGATTACCTG 
CGCTGTACTG CTGGATCTAG TATACCAGCA GCAGTGGTGA GCTTCGCCAT TGCCAAGAAC 
AGACAAGTCG CAGTCTCAGA CTTTCAACTG GTCTATGTGT CTTCCTTTGC TGTGACAACC 
ACGTGTCTGG TGTGGTTTGG CTGTAAACTG GCCATCAGTC CTTCAGCCAT CAATATTAAC 
TTTAACCTGC TCCTGCTTAT TGTGCTGGAA GTGCTCACCG CCAGTACTGT TATTCTGTCA 
GCACGTTCAG TGGACGACTG CTGCAGTCAC AGCAAGCCGG TGTCTGAAGG CCCAATGATT 
GTGACCCAAA CATCGTTCCC CACTCGTCTT TTCAAGGCCT TCGCCGTAAT TGAGCTCATA 
GTGGGCATCG CTGCAGTTTT TGGAGGAATT ATTGCCCTCA ATTTGGGCGC TCTTCTCCCA 
GAGCCTTACC TTTCTGTTAC CTTCTTCTGG ATTCTGGCAG CGTGTTTCCC CAGTGCCATC 
GCCAGCCATG TGGTGGCAGA AAATCCCAGC AAAAGTCTGG TGGAAATGCT GATTGCTATA 
AGTAGTGTGA CTTCACCACT GCTCTTCTCG GCCTCTGCAT TCTTATCCAG CAGTGTGATT 
AGTTTTATTG AGATCTTTCT GCACGAAGTA GCATTAGCCA AGCAGTCGTA TGACATCCTG 
CTGTTGGTCC TGATGCTGCT GCTGCTGGTG CCAGCGGTGC TGGCTTTAGC CACTATTATA 
CAGTGTGCTT CCTACAAGAG CCAGGTCCGC ATGGGTGCTA ACGAATGGGA CACACTGCGC 
AAAAAACACT GTGAGCAGCT GGCATCAAAT GGCACATTAA GGGATTTCGA CAAAGAAAAG 
GCCTGGAAGG CAGTGGTCGT CCAGATGGCC CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001098409.1
CDS204..1379
Translation

Target ORF information:

RefSeq Version NM_001104939.1
Organism Danio rerio(zebrafish)
Definition Danio rerio modulator of VRAC current 1 (mlc1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001104939.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCAGCGAG AGGAGGCATC GGCTCAGGAA GTGTTCAGCT ATGCCCAGAT GTCCACCCTG 
GAGGGAAACA GCGGTGTAGG AACGCTGAGT CGGCATCTAG AGCGCGAGCG ATTGGAGAGA 
GACGGCTACA CGGTGGATGT CAGAGCCAGT GACCTGCAGC TGGATCAGCC TGGCCCGCTA 
CATCCCTGCT TCAGCTACAG AGTCTGGCTT TACAGCGTGG TTGTAGGGAG CAGCTTGCTG 
ATAGCGTCGG CTTTCTCGCT CTACATGGGT AATGTGTTTC CTGCTGCAAT GGATTACCTG 
CGCTGTACTG CTGGATCTAG TATACCAGCA GCAGTGGTGA GCTTCGCCAT TGCCAAGAAC 
AGACAAGTCG CAGTCTCAGA CTTTCAACTG GTCTATGTGT CTTCCTTTGC TGTGACAACC 
ACGTGTCTGG TGTGGTTTGG CTGTAAACTG GCCATCAGTC CTTCAGCCAT CAATATTAAC 
TTTAACCTGC TCCTGCTTAT TGTGCTGGAA GTGCTCACCG CCAGTACTGT TATTCTGTCA 
GCACGTTCAG TGGACGACTG CTGCAGTCAC AGCAAGCCGG TGTCTGAAGG CCCAATGATT 
GTGACCCAAA CATCGTTCCC CACTCGTCTT TTCAAGGCCT TCGCCGTAAT TGAGCTCATA 
GTGGGCATCG CTGCAGTTTT TGGAGGAATT ATTGCCCTCA ATTTGGGCGC TCTTCTCCCA 
GAGCCTTACC TTTCTGTTAC CTTCTTCTGG ATTCTGGCAG CGTGTTTCCC CAGTGCCATC 
GCCAGCCATG TGGTGGCAGA AAATCCCAGC AAAAGTCTGG TGGAAATGCT GATTGCTATA 
AGTAGTGTGA CTTCACCACT GCTCTTCTCG GCCTCTGCAT TCTTATCCAG CAGTGTGATT 
AGTTTTATTG AGATCTTTCT GCACGAAGTA GCATTAGCCA AGCAGTCGTA TGACATCCTG 
CTGTTGGTCC TGATGCTGCT GCTGCTGGTG CCAGCGGTGC TGGCTTTAGC CACTATTATA 
CAGTGTGCTT CCTACAAGAG CCAGGTCCGC ATGGGTGCTA ACGAATGGGA CACACTGCGC 
AAAAAACACT GTGAGCAGCT GGCATCAAAT GGCACATTAA GGGATTTCGA CAAAGAAAAG 
GCCTGGAAGG CAGTGGTCGT CCAGATGGCC CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa23165
Clone ID Related Accession (Same CDS sequence) XM_005169941.3 , XM_017351934.2 , XM_005169941.4 , XM_021467665.1 , XM_017351934.1
Accession Version XM_005169941.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product membrane protein MLC1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169941.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
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181
241
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361
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481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCAG CTGGCATCAA ATGGCACATT AAGGGATTTC 
GACAAAGAAA AGGCCTGGAA GGCAGTGGTC GTCCAGATGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005169998.1
CDS289..1476
Translation

Target ORF information:

RefSeq Version XM_005169941.3
Organism Danio rerio(Zebrafish)
Definition Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005169941.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCAG CTGGCATCAA ATGGCACATT AAGGGATTTC 
GACAAAGAAA AGGCCTGGAA GGCAGTGGTC GTCCAGATGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa23165
Clone ID Related Accession (Same CDS sequence) XM_005169941.3 , XM_017351934.2 , XM_005169941.4 , XM_021467665.1 , XM_017351934.1
Accession Version XM_017351934.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product membrane protein MLC1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCAG CTGGCATCAA ATGGCACATT AAGGGATTTC 
GACAAAGAAA AGGCCTGGAA GGCAGTGGTC GTCCAGATGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017207423.1
CDS273..1460
Translation

Target ORF information:

RefSeq Version XM_017351934.2
Organism Danio rerio(zebrafish)
Definition Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017351934.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCAG CTGGCATCAA ATGGCACATT AAGGGATTTC 
GACAAAGAAA AGGCCTGGAA GGCAGTGGTC GTCCAGATGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa23165
Clone ID Related Accession (Same CDS sequence) XM_005169941.3 , XM_017351934.2 , XM_005169941.4 , XM_021467665.1 , XM_017351934.1
Accession Version XM_005169941.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product membrane protein MLC1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169941.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCAG CTGGCATCAA ATGGCACATT AAGGGATTTC 
GACAAAGAAA AGGCCTGGAA GGCAGTGGTC GTCCAGATGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005169998.1
CDS294..1481
Translation

Target ORF information:

RefSeq Version XM_005169941.4
Organism Danio rerio(zebrafish)
Definition Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005169941.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCAG CTGGCATCAA ATGGCACATT AAGGGATTTC 
GACAAAGAAA AGGCCTGGAA GGCAGTGGTC GTCCAGATGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa23165
Clone ID Related Accession (Same CDS sequence) XM_005169941.3 , XM_017351934.2 , XM_005169941.4 , XM_021467665.1 , XM_017351934.1
Accession Version XM_021467665.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product membrane protein MLC1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCAG CTGGCATCAA ATGGCACATT AAGGGATTTC 
GACAAAGAAA AGGCCTGGAA GGCAGTGGTC GTCCAGATGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_021323340.1
CDS118..1305
Translation

Target ORF information:

RefSeq Version XM_021467665.1
Organism Danio rerio(zebrafish)
Definition Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_021467665.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCAG CTGGCATCAA ATGGCACATT AAGGGATTTC 
GACAAAGAAA AGGCCTGGAA GGCAGTGGTC GTCCAGATGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa23165
Clone ID Related Accession (Same CDS sequence) XM_005169941.3 , XM_017351934.2 , XM_005169941.4 , XM_021467665.1 , XM_017351934.1
Accession Version XM_017351934.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product membrane protein MLC1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCAG CTGGCATCAA ATGGCACATT AAGGGATTTC 
GACAAAGAAA AGGCCTGGAA GGCAGTGGTC GTCCAGATGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017207423.1
CDS268..1455
Translation

Target ORF information:

RefSeq Version XM_017351934.1
Organism Danio rerio(Zebrafish)
Definition Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017351934.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCAG CTGGCATCAA ATGGCACATT AAGGGATTTC 
GACAAAGAAA AGGCCTGGAA GGCAGTGGTC GTCCAGATGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa23167
Clone ID Related Accession (Same CDS sequence) XM_005169943.3 , XM_005169943.4
Accession Version XM_005169943.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1185bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product membrane protein MLC1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169943.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCTG GCATCAAATG GCACATTAAG GGATTTCGAC 
AAAGAAAAGG CCTGGAAGGC AGTGGTCGTC CAGATGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005170000.1
CDS246..1430
Translation

Target ORF information:

RefSeq Version XM_005169943.3
Organism Danio rerio(Zebrafish)
Definition Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005169943.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCTG GCATCAAATG GCACATTAAG GGATTTCGAC 
AAAGAAAAGG CCTGGAAGGC AGTGGTCGTC CAGATGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa23167
Clone ID Related Accession (Same CDS sequence) XM_005169943.3 , XM_005169943.4
Accession Version XM_005169943.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1185bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product membrane protein MLC1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169943.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCTG GCATCAAATG GCACATTAAG GGATTTCGAC 
AAAGAAAAGG CCTGGAAGGC AGTGGTCGTC CAGATGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005170000.1
CDS252..1436
Translation

Target ORF information:

RefSeq Version XM_005169943.4
Organism Danio rerio(zebrafish)
Definition Danio rerio megalencephalic leukoencephalopathy with subcortical cysts 1 (mlc1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005169943.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTGA GGATGCAGCG AGAGGAGGCA TCGGCTCAGG AAGTGTTCAG CTATGCCCAG 
ATGTCCACCC TGGAGAGAAA CAGCGGTGTA GGAACGCTGA GTCGGCATCT AGAGCGCGAG 
CGATTGGAGA GAGACGGCTA CACGGTGGAT GTCAGAGCCA GTGACCTGCA GCTGGATCAG 
CCTGGCCCGC TACATCCCTG CTTCAGCTAC AGAGTCTGGC TTTACAGTGT GGTTGTAGGG 
AGCAGCTTGC TGATAGCGTC GGCTTTCTCG CTCTACATGG GTAATGTGTT TCCTGCTGCA 
ATGGATTACC TGCGCTGTAC TGCTGGATCT AGTATACCAG CAGCAGTGGT GAGCTTCGCC 
ATTGCCAAGA ACAGACAAGT CGCAGTCTCA GACTTTCAAC TGGTCTATGT GTCTTCCTTT 
GCTGTGACAA CCACGTGTCT GGTGTGGTTT GGCTGTAAAC TGGCCATCAG TCCTTCAGCC 
ATCAATATTA ACTTTAACCT GCTCCTGCTT ATTGTGCTGG AAGTGCTCAC CGCCAGTACT 
GTTATTCTGT CAGCACGTTC AGTGGACAAC TGCTGCAGTC ACAGCAAGCC GGTGTCTGAA 
GGCCCAATGA TTGTGACCCA AACATCGTTC CCCACTCGTC TTTTCAAGGC CTTCGCCGTA 
ATTGAGCTCA TAGTGGGCAT CGCTGCAGTT TTTGGAGGAA TTATTGCCCT CAATTTGGGC 
GCTCTTCTCC CAGAGCCTTA CCTTTCTGTT ACCTTCTTCT GGATTCTGGC AGCGTGTTTC 
CCCAGTGCCA TCGCCAGCCA TGTGGTGGCA GAAAATCCCA GCAAAAGTCT GGTGGAAATG 
CTGATTGCTA TAAATAGTGT GACTTCACCA CTGCTCTTCT CGGCCTCTGC ATTCTTATCC 
AGCAGTGTGA TTAGTTTTAT TGAGATCTTT CTGCACGAAG TAGCATTAGC CAAGCAGTCG 
TATGACATCC TGCTGTTGGT CCTGATGCTG CTGCTGCTGG TGCCAGCGGT GCTGACTTTA 
GCCACTATTA TACAGTGTGC TTCCTACAAG AGCCAGGTCC GCATGGGTGC TAACGAATGG 
GACACACTGC GCAAAAAACA CTGTGAGCTG GCATCAAATG GCACATTAAG GGATTTCGAC 
AAAGAAAAGG CCTGGAAGGC AGTGGTCGTC CAGATGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG


book

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J


book

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R


book

Megalencephalic leukoencephalopathy with subcortical cysts protein 1 regulates glial surface localization of GLIALCAM from fish to humans.
Human molecular genetics23(19)5069-86(2014 Oct)
Sirisi S,Folgueira M,L?pez-Hern?ndez T,Minieri L,P?rez-Rius C,Gait?n-Pe?as H,Zang J,Mart?nez A,Capdevila-Nortes X,De La Villa P,Roy U,Alia A,Neuhauss S,Ferroni S,Nunes V,Est?vez R,Barrallo-Gimeno A