rlf cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rlf
Entrez Gene ID	562452
Full Name	rearranged L-myc fusion
Synonyms	im:7146976,si:ch211-154e15.8
General protein information	Preferred Names rearranged L-myc fusion Names zinc finger protein Rlf
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_685832.8	XP_690924.6	zinc finger protein Rlf
XM_685832.9	XP_690924.7	zinc finger protein Rlf
XM_021467415.1	XP_021323090.1	zinc finger protein Rlf

Pathways from BioSystems

Homologies

Homo sapiens (human)	RLF	NP_036553.2
Pan troglodytes (chimpanzee)	RLF	XP_524678.1
Rattus norvegicus (Norway rat)	Rlf	XP_233485.6
Danio rerio (zebrafish)	rlf	XP_690924.6
	LOC693503	XP_002808256.1
Bos taurus (cattle)	RLF	NP_001192868.1
Xenopus tropicalis (tropical clawed frog)	rlf	NP_001123386.2
Canis lupus familiaris (dog)	RLF	XP_848535.1
Mus musculus (house mouse)	Rlf	NP_001074482.1
Gallus gallus (chicken)	RLF	XP_417721.4

Gene Ontology

The following rlf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rlf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145913	XM_685832.9 Latest version!	Danio rerio rearranged L-myc fusion (rlf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa22526	XM_021467415.1 Latest version!	Danio rerio rearranged L-myc fusion (rlf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa22526	XM_685832.8 Latest version!	Danio rerio rearranged L-myc fusion (rlf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rlf ( XM_685832.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_690924.7 Danio rerio rearranged L-myc fusion (rlf), mRNA.

Price & Availability↑

CloneID	ODa145913
Clone ID Related Accession (Same CDS sequence)	XM_685832.9
Accession Version	XM_685832.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein Rlf
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394990.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_685832.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCT GTGCGTACGC GCCGTGGCTG CCAGTGAACA TGGCGGATGA GAATGCTGAA 
GCGGAGCTCG ACTGGGGCGG CCGACACAGC CTGCTGGTCG ACGAGGACAC ATTTTTCGCC 
ATGGAGGGTT TGCAAGCAAC CCTTCAGCAA CACGAAGCCG AGCTTCGACA GCAAGACGTT 
TCTGAGGAAT CGTCCACGGG TTATTGCAAC AACTTCTGCG AGGCTCTAAT GCATTATGCT 
GGGAGCAGGA ATTCGTTGGA ACATGGATTG CCTCTATTGG AAGTTTACTG TCTTTCAATC 
AACTGTTTTG CTGCTGCCAG GCCACATCTC ACAGGCGACT CCCCCAGTGT TGCCCTTGTG 
CTTAAAAGAC TTGCATTGAG TTGCCTTGAA TTGTTGTTGT CAGTGCCTCA AAATAATATA 
CCTTTGGAAG CCTGGCTGCA GTTCCATGGC TCAGTTCAGG CGGCCCATGA AGCCATGCTG 
CAGTATGGAA GTACAGATTT GCATGCCCTG CGGAACATCA CTGGTGAAGG TGGAGCTTGG 
AACAATTCTG TCATGGTCTC GCTTCTCACC GGTCAGCCCA CAGACCCGGA TGAGGTTAAT 
GCCTATCTAG CTCTGGAGGG AGATGATTTC ATGGAAATGC GAATCAAACA TCTGGAGAAA 
GTAGGCGAGC TAGAGAAAGC ACTGATCCTC ATCAAAGCCT GTGCTAACTG TAGCCTTCTG 
TCTAACCAGT CAACATTTCG CCAAACCTTT GTAACCCAAC TTTGCCAGCT GCTGCCCAGT 
GAAGAAGCTA TTTTAGAGAT TTCTAGAATA GATGCAAAAG ATGTCCTGGA TATTATTTGC 
AACCTAGATG CTGAGGGCGA TGAGAGAACA GCCTTCATCT TATGCACAAC GTATTTTACA 
CAGCAGCTAC AGCAAGACAG CACATACTGT TCCTGGGAGT TAACCCTCCT TTGGAGTAAA 
CTGCAGAGAA GAATTGATGT GTCCCTTGAG TCATTTATCG AACGCTGTCT CCAGTTTGCA 
GCCATCGCCA GGACTATCTA CCATCTTCTT TTCCTTATTC GTGTAATTCA AACTGAGGCA 
ATGCAGCTTG GCCTTGCAGT ATCAGTTGAG CTGTGTGTGA AGGCTCTACG CCTTCCAAGG 
CAAGATGACA TTGAAGCAAA AACAATGGTC TGCAAAGCTG TCGTCTGTCT TCTCCCTGAT 
GATCTTGAGG TGATACGGGC TTGCCAGCTT ACAGAATTCC TCTTGAGCCC ATCCCAGGAA 
GCATTTGATA TCCTTGAGGA GCTCTATCTG CGTCCTGATG AAAAATATGA TGAGGAAAAC 
GCCATAATTC CAAATTTCCT GCGCTGTGAG CTGCTACTTT CTCTGAAAGC ACATTGGCCC 
ATTGACCCTG AATTCTGGGA CTGGAAAACT TTAAAACGCT ACTGCATTCA GTTGCTGGGG 
CTGGAACCTG TGGATGAAAT TGAAGATGAA GCAGCACCTG GGGAGCTATG TAATCTTGGA 
GTGGTTTTGA ATGAAGAGGC TGGGAAGGAG GAGGAAGACG AATGTGAGCT TGATGCGCTC 
AGCAGCGCAG GGGAGCAGCA AACTGTGGAA GAATCAGAAA AGGGACAAAA AGATTCAGAA 
GATGTCTCTA AGTTAGCTGA TAAGCATCAG AGGGATAAAT TTCTCTGCCA GATATGCCAT 
AAAGAAGTGG CAGAGACCCG GTTCTGTCAC CACGCCAGAA AACATATGGA GGATGGCATG 
TGGACATGCC CTGTGTGTCT ACAAAAGTTT AAAACTAAAA AGGAGTTTGA GCCACACTCC 
AAAAAACACA TTCAGATTTC TGCAAAAGAT CACTTTAAAA AGAAAAAAGT GAAGAAGAAG 
GTGGACCAGA CTAAAGATTT CTTCAAGGAG GATAGTTCTG ATGAGCTAGA GCCTGGTCAA 
ATTCCTTTAG ACCCTTCACT TGCCATGTAC TACCAGTCCA CCCATGATCC TGTAGTATTG 
GAACATATAT TAGAGAAAGC TGCCAGTGTG CCTGAAAAGC AAGTAGAAAG TGACTATGTT 
ACATTTGACT ACATCAACAC GTACTTTAAG TTACAGGATC GTGATGTTTA CCAGTGTCCA 
GCCACTGACT GCTCAAAGAA TTTTAAACTT TTCAAGTACC TGGGTGTGCA CATCAAAAAT 
GAGCATGATA GTGAAGACCC CAATTTGAAA CATTATCTTA AGATGAAAGA CTGTCGGGAG 
AAATGCACTT TCTGCCGGAA GACATTCATG ACCGCCTATC ACCATCGACT GCACCGTTGG 
ATTCATTATG GGGATGATCC TTACATGTGT GTGGTCACAG GTTGTGGTGC CCGCTTTGAC 
ACCACCAATG AACTTTTAGC ACATAAGCAT GGCCATGGGT TTCGATTGTC CTACTGTTGT 
GAGTTAAAAG GCTGCAGTTT CTCCTACTGT GACCTTGGGC AGCTTTACCA CCATGAGGCG 
CAGCACTTTC GTGATGCTGC TTACACTTGT ACCAGCCCGG GGTGCAAGAA CTTTTACTAT 
TCTCGCAAAG AATTTCTGCA ACACTTGGCC ACTCATGACA TCACTTTCAC AGAAAAAGAC 
TTTGAGGCTC AGAGAAAAGC AAAAAGGAAA CTCTTGCTCG CAGTTGTAGA AAGTGATTCG 
GCTTTTGGAT CAAAGGTTGA AACAGAGAAT TGTGTCCAGA AAAGTGGTTC AAGTTCATCT 
TCACAGACCT TGGCAAAGGG TCGATCATCA GTCTCGCAAA CATGTGTTGC TGTCTGCTTT 
GATGGTAAAA AGTTTACATG TGGCTTAAAA AAGTGTGGAA AAACATACAC TACTGCAAGA 
GAACTTCAGA AGCACTTGAG AATTGCTCAT TCAGACACAT TTAATGAGAA GGATCAGTTA 
TGCAAAAAGA AAGTACAAGT AAAGCATTCT ACGACTCGAA ATGAAGATTT ACCTAAAAGT 
GACCCGGATA ACACTGTAAA AACGTCATCT ACAGAAAAAT CTAGTCCCAA AAAGGCTCTC 
TCACCTGCGT CTGAGTCTGT AGATTGTGAG ACAACCAAAT CCAATTTGGA CCTTGATGCA 
GCACTAACCA ATATCATGCT TGGATTGGGC CAATTAAACC TTAATTCTTC CAACACCAGA 
CATTCTATGT GTAATTCACA GAGCACTTGT ACACCTACCT TGAATACAAG GTCTCCTGCC 
AAAGCAGCAC ACAGCAAAAA TGAGTCAAGA TCTCACGTCA CAGGTAGTGA ATCAAAAAAG 
CTGCCAAACA AGTCAGAATC ACCAAAAACA CATGACAATA ATAAAGATGT ATCTGATAAG 
AAGCAAAAAA AAGAATCTGT CTCAGAGGAG GAACAACCAC AAAGCAAGTC CTTAACCAAG 
CCTTATAACT GCGAAGCCAA AAGCTGCCAC TATCAGAGCA CTACCAGTTG TGCTTTAATG 
CAGCACTATA TTAAGGTTCA TGGTTACTCC GAGAAGATGG TGAAAGAGAT GGAGGTGTTT 
CAATCTGAGA CATTGGAGCC TTTCAAAACA CCTGACAGCT CTGAAAATTC GGTCTTGGAG 
GATGATCAAT CACAAAGCGA GTTTCTTGTG CAGGCTACAG TCAAGCCTTA TGCCTGTGAA 
GCGAAGAGTT GCAACTACCA GAGTGTTACT AGTCATGCTT TATTGAAGCA CTACATCAAG 
ATTCATGGGT ACTCCGAAAA GAAGGTGAAA AATATGGAGG TGTTTCAGTC TCAGATATTC 
AAGCCTTTTA AGTGCCATTT GTGCTCCAAG AGTTACAGAA ACAAAAAAGA ACTGAGGCGT 
CATTATATTC AAATGCATCA CATAAAAGAA GACATTGTTG AACAAATGAA GTGCTCTTTT 
AAAAAGAATG TAGTTGAAAA AGCACCAGTG TTGTCATCAC CTAAAAAAAA CACTGAGGTA 
AAGCCACAGT CTTCAAAGAA GGCTGATGTT AAAACCCAAA AACAAGACAA TGCCCTTTGG 
CAACAGAAAT GCCCAAAACA TGATTATCGG TTGTGGGTCA GAAAAGTAGT TACGAAAAAC 
GTGCATGTTA AAGCTGAAAA AAAAAAGAAA GCCTCTTCGG AAACTCGATC TGATGTAAAA 
AACACAACGG TTGATGTTCG GGGAAGCCGA CGCTTAATTT CAAAAGGGAA TCTGAGCTAC 
ATCCTCACTA AATACAATAA ACCCTTTCAC TGCGTGCACA AAAACTGCAA TGCAGCCTTC 
AATAACCAGA ATGCCCTTGT TGGCCACCTT CAGATGGTGC ATCATTATAA CCGATCACAG 
TTGTGCTTTC CATGTGAACA CAAAGGATGC GAGAAGCAGT ATAGCACCTT ATCTAGTCTT 
ACTAAACACT ACAGAAGAGA CCACAACTTC ACCAAAAAAG AGAAAGAAAG GAAAATTCTG 
CCGAAACTGC CCTGTACATC TAAAAGTATA CCAAAAAGAC AGATGGCCTC AACAGAGGAG 
CCCATACCAA GGTTCAAGTG TACCTACGCT AATTGCAATG AGTCCTACCA TCTCAACAGC 
AGCCTCTTGC GTCACACAAG TCAGTTTCAC CAAAACCAGA CCCCCCAGAA TTCGGCTAAT 
ACTTCAGGGA ACAACGAGTT TAAAACTAGT TATAAGAAGC ACGTGTTCTA TAGGCACTGT 
GATCCGTCTG ATTCCCTTGT GGTGCGTCTC CAGAGTACAC CCAAGAAAGA AGAGTCAAAA 
AATGGATGCC AAACGAAGTT AATCATCTCA CCACCTTCCC AGTCATCAAA AGATCCCCAA 
AATATGCCTC TCAAGCAATC TTTGAGATGC TGCCGCAATG ATCAGAAAAT CAATTCCTCT 
GAGAATTTTG AGGGTGTTGA AGAAACACCA GAAGAGGAGC TGGACGAAGT ACTTAAGCCG 
CAAAGAATCA ACCTAAAAAA TAGATTTGAC CTGATTGTCT ACCGAACACA TGAGGAGGCT 
TTGCAGATGT GCCAAGACCG CTGCCTTCCC GTGGCCTTTC CTTGCATGAT ACAAAACTGT 
GACTCTGTTG TCGTAAGCAT GCATAGTTTG AATCGACATT ACATTAATGT TCACAGGTTT 
AAACGCAAAC AGCTGACGGA AAATAAGGAC AAATTATGTT TCACTGCTGA ACAGTTGGAG 
GAGATCATAC AAAAGAAGTC TGCTGTGTCT GCTATACCTG ATTTAACAAG GATTCCAAAT 
GGCGTTCTCA AGATGGAGTA TCAGTCTGAG CCTGCCACTC CAGGTGGCCC GTCTCAACCA 
ATGAGCCTCC ATTCGATCAA AACAGATTCA AAGGAGAAAG AAGGGATTGA GTTTTCAGAA 
GAGCCGCTCC CTGACAGAAG TTTGCTCATT GAAGCTGAGG ATTTGCTCTA TGGCTCCCCA 
AAGTCAAGCG GGAACCCCGA GAAGCCTGTT TCTGAAGAGA GTCTCAACCA CAAAGATGAG 
CCAGAATCTG AATTAACAGC ACCTCCCCTC ATACGCCCCC CACCACTTGA CCTCTCTCCA 
CCTGCTACAC TTAGAATCAC AGTTGATGAA AGTTCATTTG AAGCTTCTGG TAAAGACAGT 
AAATCTATTA ATATTCCTTC TAGTGTTTCC ATATCGGTCC CCACCCCAAC TCGGCAGCCC 
CTCAGACGAA AGAATGAGCT CTCTGAACCA CCACTACCAC CAGTCCCTCT TTCTCCAGTC 
CCAATGGATC CTGTAGAGCA CAGTCTATCC CCAAGGGCTT TTGACATTGC AGCATACAAA 
CCTATGGGCT TCGAGTCATC ATTCTTGAAG TTCATTAAAG ACAAGGATGA GGAGATCAAA 
CTTGAAAAAC CATGGACTGC AGTAGTTTCT AATCCTTGCC TCAAGCCTGA TCCAAATCGC 
CGGCGAGATT GTTTCAGACG AAATTGCTCA GTCAAGGAGA ACACTGAAAG TGGAGCCCCC 
ATTTCTCGAA GCCGCAGGTT TCGGTCATCC CCTTTGAGGC ATCTGATTTC TAAAGGAGAG 
TGTACCTCTA TTCAGAATCT CCGTATGATT TTAGAGAGAG CTTTAGGGGG GTGTGGCGAC 
CAAGCTATAA AACAGCTACA GTTCCTGAAG CCTGTGGTGG TTCTAGAGAG GCCAAAGTCC 
TTTGCCTCCA TTTTCGATCT CTTGCCCTCT GAAACTAAAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690924.7
CDS	14..6118
Translation	MTACAYAPWLPVNMADENAEAELDWGGRHSLLVDEDTFFAMEGLQATLQQHEAELRQQDVSEESSTGYCNNFCEALMHYAGSRNSLEHGLPLLEVYCLSINCFAAARPHLTGDSPSVALVLKRLALSCLELLLSVPQNNIPLEAWLQFHGSVQAAHEAMLQYGSTDLHALRNITGEGGAWNNSVMVSLLTGQPTDPDEVNAYLALEGDDFMEMRIKHLEKVGELEKALILIKACANCSLLSNQSTFRQTFVTQLCQLLPSEEAILEISRIDAKDVLDIICNLDAEGDERTAFILCTTYFTQQLQQDSTYCSWELTLLWSKLQRRIDVSLESFIERCLQFAAIARTIYHLLFLIRVIQTEAMQLGLAVSVELCVKALRLPRQDDIEAKTMVCKAVVCLLPDDLEVIRACQLTEFLLSPSQEAFDILEELYLRPDEKYDEENAIIPNFLRCELLLSLKAHWPIDPEFWDWKTLKRYCIQLLGLEPVDEIEDEAAPGELCNLGVVLNEEAGKEEEDECELDALSSAGEQQTVEESEKGQKDSEDVSKLADKHQRDKFLCQICHKEVAETRFCHHARKHMEDGMWTCPVCLQKFKTKKEFEPHSKKHIQISAKDHFKKKKVKKKVDQTKDFFKEDSSDELEPGQIPLDPSLAMYYQSTHDPVVLEHILEKAASVPEKQVESDYVTFDYINTYFKLQDRDVYQCPATDCSKNFKLFKYLGVHIKNEHDSEDPNLKHYLKMKDCREKCTFCRKTFMTAYHHRLHRWIHYGDDPYMCVVTGCGARFDTTNELLAHKHGHGFRLSYCCELKGCSFSYCDLGQLYHHEAQHFRDAAYTCTSPGCKNFYYSRKEFLQHLATHDITFTEKDFEAQRKAKRKLLLAVVESDSAFGSKVETENCVQKSGSSSSSQTLAKGRSSVSQTCVAVCFDGKKFTCGLKKCGKTYTTARELQKHLRIAHSDTFNEKDQLCKKKVQVKHSTTRNEDLPKSDPDNTVKTSSTEKSSPKKALSPASESVDCETTKSNLDLDAALTNIMLGLGQLNLNSSNTRHSMCNSQSTCTPTLNTRSPAKAAHSKNESRSHVTGSESKKLPNKSESPKTHDNNKDVSDKKQKKESVSEEEQPQSKSLTKPYNCEAKSCHYQSTTSCALMQHYIKVHGYSEKMVKEMEVFQSETLEPFKTPDSSENSVLEDDQSQSEFLVQATVKPYACEAKSCNYQSVTSHALLKHYIKIHGYSEKKVKNMEVFQSQIFKPFKCHLCSKSYRNKKELRRHYIQMHHIKEDIVEQMKCSFKKNVVEKAPVLSSPKKNTEVKPQSSKKADVKTQKQDNALWQQKCPKHDYRLWVRKVVTKNVHVKAEKKKKASSETRSDVKNTTVDVRGSRRLISKGNLSYILTKYNKPFHCVHKNCNAAFNNQNALVGHLQMVHHYNRSQLCFPCEHKGCEKQYSTLSSLTKHYRRDHNFTKKEKERKILPKLPCTSKSIPKRQMASTEEPIPRFKCTYANCNESYHLNSSLLRHTSQFHQNQTPQNSANTSGNNEFKTSYKKHVFYRHCDPSDSLVVRLQSTPKKEESKNGCQTKLIISPPSQSSKDPQNMPLKQSLRCCRNDQKINSSENFEGVEETPEEELDEVLKPQRINLKNRFDLIVYRTHEEALQMCQDRCLPVAFPCMIQNCDSVVVSMHSLNRHYINVHRFKRKQLTENKDKLCFTAEQLEEIIQKKSAVSAIPDLTRIPNGVLKMEYQSEPATPGGPSQPMSLHSIKTDSKEKEGIEFSEEPLPDRSLLIEAEDLLYGSPKSSGNPEKPVSEESLNHKDEPESELTAPPLIRPPPLDLSPPATLRITVDESSFEASGKDSKSINIPSSVSISVPTPTRQPLRRKNELSEPPLPPVPLSPVPMDPVEHSLSPRAFDIAAYKPMGFESSFLKFIKDKDEEIKLEKPWTAVVSNPCLKPDPNRRRDCFRRNCSVKENTESGAPISRSRRFRSSPLRHLISKGECTSIQNLRMILERALGGCGDQAIKQLQFLKPVVVLERPKSFASIFDLLPSETKV

Target ORF information:

RefSeq Version	XM_685832.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rearranged L-myc fusion (rlf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685832.9

ORF Insert Sequence:

ATGACCGCCT GTGCGTACGC GCCGTGGCTG CCAGTGAACA TGGCGGATGA GAATGCTGAA 
GCGGAGCTCG ACTGGGGCGG CCGACACAGC CTGCTGGTCG ACGAGGACAC ATTTTTCGCC 
ATGGAGGGTT TGCAAGCAAC CCTTCAGCAA CACGAAGCCG AGCTTCGACA GCAAGACGTT 
TCTGAGGAAT CGTCCACGGG TTATTGCAAC AACTTCTGCG AGGCTCTAAT GCATTATGCT 
GGGAGCAGGA ATTCGTTGGA ACATGGATTG CCTCTATTGG AAGTTTACTG TCTTTCAATC 
AACTGTTTTG CTGCTGCCAG GCCACATCTC ACAGGCGACT CCCCCAGTGT TGCCCTTGTG 
CTTAAAAGAC TTGCATTGAG TTGCCTTGAA TTGTTGTTGT CAGTGCCTCA AAATAATATA 
CCTTTGGAAG CCTGGCTGCA GTTCCATGGC TCAGTTCAGG CGGCCCATGA AGCCATGCTG 
CAGTATGGAA GTACAGATTT GCATGCCCTG CGGAACATCA CTGGTGAAGG TGGAGCTTGG 
AACAATTCTG TCATGGTCTC GCTTCTCACC GGTCAGCCCA CAGACCCGGA TGAGGTTAAT 
GCCTATCTAG CTCTGGAGGG AGATGATTTC ATGGAAATGC GAATCAAACA TCTGGAGAAA 
GTAGGCGAGC TAGAGAAAGC ACTGATCCTC ATCAAAGCCT GTGCTAACTG TAGCCTTCTG 
TCTAACCAGT CAACATTTCG CCAAACCTTT GTAACCCAAC TTTGCCAGCT GCTGCCCAGT 
GAAGAAGCTA TTTTAGAGAT TTCTAGAATA GATGCAAAAG ATGTCCTGGA TATTATTTGC 
AACCTAGATG CTGAGGGCGA TGAGAGAACA GCCTTCATCT TATGCACAAC GTATTTTACA 
CAGCAGCTAC AGCAAGACAG CACATACTGT TCCTGGGAGT TAACCCTCCT TTGGAGTAAA 
CTGCAGAGAA GAATTGATGT GTCCCTTGAG TCATTTATCG AACGCTGTCT CCAGTTTGCA 
GCCATCGCCA GGACTATCTA CCATCTTCTT TTCCTTATTC GTGTAATTCA AACTGAGGCA 
ATGCAGCTTG GCCTTGCAGT ATCAGTTGAG CTGTGTGTGA AGGCTCTACG CCTTCCAAGG 
CAAGATGACA TTGAAGCAAA AACAATGGTC TGCAAAGCTG TCGTCTGTCT TCTCCCTGAT 
GATCTTGAGG TGATACGGGC TTGCCAGCTT ACAGAATTCC TCTTGAGCCC ATCCCAGGAA 
GCATTTGATA TCCTTGAGGA GCTCTATCTG CGTCCTGATG AAAAATATGA TGAGGAAAAC 
GCCATAATTC CAAATTTCCT GCGCTGTGAG CTGCTACTTT CTCTGAAAGC ACATTGGCCC 
ATTGACCCTG AATTCTGGGA CTGGAAAACT TTAAAACGCT ACTGCATTCA GTTGCTGGGG 
CTGGAACCTG TGGATGAAAT TGAAGATGAA GCAGCACCTG GGGAGCTATG TAATCTTGGA 
GTGGTTTTGA ATGAAGAGGC TGGGAAGGAG GAGGAAGACG AATGTGAGCT TGATGCGCTC 
AGCAGCGCAG GGGAGCAGCA AACTGTGGAA GAATCAGAAA AGGGACAAAA AGATTCAGAA 
GATGTCTCTA AGTTAGCTGA TAAGCATCAG AGGGATAAAT TTCTCTGCCA GATATGCCAT 
AAAGAAGTGG CAGAGACCCG GTTCTGTCAC CACGCCAGAA AACATATGGA GGATGGCATG 
TGGACATGCC CTGTGTGTCT ACAAAAGTTT AAAACTAAAA AGGAGTTTGA GCCACACTCC 
AAAAAACACA TTCAGATTTC TGCAAAAGAT CACTTTAAAA AGAAAAAAGT GAAGAAGAAG 
GTGGACCAGA CTAAAGATTT CTTCAAGGAG GATAGTTCTG ATGAGCTAGA GCCTGGTCAA 
ATTCCTTTAG ACCCTTCACT TGCCATGTAC TACCAGTCCA CCCATGATCC TGTAGTATTG 
GAACATATAT TAGAGAAAGC TGCCAGTGTG CCTGAAAAGC AAGTAGAAAG TGACTATGTT 
ACATTTGACT ACATCAACAC GTACTTTAAG TTACAGGATC GTGATGTTTA CCAGTGTCCA 
GCCACTGACT GCTCAAAGAA TTTTAAACTT TTCAAGTACC TGGGTGTGCA CATCAAAAAT 
GAGCATGATA GTGAAGACCC CAATTTGAAA CATTATCTTA AGATGAAAGA CTGTCGGGAG 
AAATGCACTT TCTGCCGGAA GACATTCATG ACCGCCTATC ACCATCGACT GCACCGTTGG 
ATTCATTATG GGGATGATCC TTACATGTGT GTGGTCACAG GTTGTGGTGC CCGCTTTGAC 
ACCACCAATG AACTTTTAGC ACATAAGCAT GGCCATGGGT TTCGATTGTC CTACTGTTGT 
GAGTTAAAAG GCTGCAGTTT CTCCTACTGT GACCTTGGGC AGCTTTACCA CCATGAGGCG 
CAGCACTTTC GTGATGCTGC TTACACTTGT ACCAGCCCGG GGTGCAAGAA CTTTTACTAT 
TCTCGCAAAG AATTTCTGCA ACACTTGGCC ACTCATGACA TCACTTTCAC AGAAAAAGAC 
TTTGAGGCTC AGAGAAAAGC AAAAAGGAAA CTCTTGCTCG CAGTTGTAGA AAGTGATTCG 
GCTTTTGGAT CAAAGGTTGA AACAGAGAAT TGTGTCCAGA AAAGTGGTTC AAGTTCATCT 
TCACAGACCT TGGCAAAGGG TCGATCATCA GTCTCGCAAA CATGTGTTGC TGTCTGCTTT 
GATGGTAAAA AGTTTACATG TGGCTTAAAA AAGTGTGGAA AAACATACAC TACTGCAAGA 
GAACTTCAGA AGCACTTGAG AATTGCTCAT TCAGACACAT TTAATGAGAA GGATCAGTTA 
TGCAAAAAGA AAGTACAAGT AAAGCATTCT ACGACTCGAA ATGAAGATTT ACCTAAAAGT 
GACCCGGATA ACACTGTAAA AACGTCATCT ACAGAAAAAT CTAGTCCCAA AAAGGCTCTC 
TCACCTGCGT CTGAGTCTGT AGATTGTGAG ACAACCAAAT CCAATTTGGA CCTTGATGCA 
GCACTAACCA ATATCATGCT TGGATTGGGC CAATTAAACC TTAATTCTTC CAACACCAGA 
CATTCTATGT GTAATTCACA GAGCACTTGT ACACCTACCT TGAATACAAG GTCTCCTGCC 
AAAGCAGCAC ACAGCAAAAA TGAGTCAAGA TCTCACGTCA CAGGTAGTGA ATCAAAAAAG 
CTGCCAAACA AGTCAGAATC ACCAAAAACA CATGACAATA ATAAAGATGT ATCTGATAAG 
AAGCAAAAAA AAGAATCTGT CTCAGAGGAG GAACAACCAC AAAGCAAGTC CTTAACCAAG 
CCTTATAACT GCGAAGCCAA AAGCTGCCAC TATCAGAGCA CTACCAGTTG TGCTTTAATG 
CAGCACTATA TTAAGGTTCA TGGTTACTCC GAGAAGATGG TGAAAGAGAT GGAGGTGTTT 
CAATCTGAGA CATTGGAGCC TTTCAAAACA CCTGACAGCT CTGAAAATTC GGTCTTGGAG 
GATGATCAAT CACAAAGCGA GTTTCTTGTG CAGGCTACAG TCAAGCCTTA TGCCTGTGAA 
GCGAAGAGTT GCAACTACCA GAGTGTTACT AGTCATGCTT TATTGAAGCA CTACATCAAG 
ATTCATGGGT ACTCCGAAAA GAAGGTGAAA AATATGGAGG TGTTTCAGTC TCAGATATTC 
AAGCCTTTTA AGTGCCATTT GTGCTCCAAG AGTTACAGAA ACAAAAAAGA ACTGAGGCGT 
CATTATATTC AAATGCATCA CATAAAAGAA GACATTGTTG AACAAATGAA GTGCTCTTTT 
AAAAAGAATG TAGTTGAAAA AGCACCAGTG TTGTCATCAC CTAAAAAAAA CACTGAGGTA 
AAGCCACAGT CTTCAAAGAA GGCTGATGTT AAAACCCAAA AACAAGACAA TGCCCTTTGG 
CAACAGAAAT GCCCAAAACA TGATTATCGG TTGTGGGTCA GAAAAGTAGT TACGAAAAAC 
GTGCATGTTA AAGCTGAAAA AAAAAAGAAA GCCTCTTCGG AAACTCGATC TGATGTAAAA 
AACACAACGG TTGATGTTCG GGGAAGCCGA CGCTTAATTT CAAAAGGGAA TCTGAGCTAC 
ATCCTCACTA AATACAATAA ACCCTTTCAC TGCGTGCACA AAAACTGCAA TGCAGCCTTC 
AATAACCAGA ATGCCCTTGT TGGCCACCTT CAGATGGTGC ATCATTATAA CCGATCACAG 
TTGTGCTTTC CATGTGAACA CAAAGGATGC GAGAAGCAGT ATAGCACCTT ATCTAGTCTT 
ACTAAACACT ACAGAAGAGA CCACAACTTC ACCAAAAAAG AGAAAGAAAG GAAAATTCTG 
CCGAAACTGC CCTGTACATC TAAAAGTATA CCAAAAAGAC AGATGGCCTC AACAGAGGAG 
CCCATACCAA GGTTCAAGTG TACCTACGCT AATTGCAATG AGTCCTACCA TCTCAACAGC 
AGCCTCTTGC GTCACACAAG TCAGTTTCAC CAAAACCAGA CCCCCCAGAA TTCGGCTAAT 
ACTTCAGGGA ACAACGAGTT TAAAACTAGT TATAAGAAGC ACGTGTTCTA TAGGCACTGT 
GATCCGTCTG ATTCCCTTGT GGTGCGTCTC CAGAGTACAC CCAAGAAAGA AGAGTCAAAA 
AATGGATGCC AAACGAAGTT AATCATCTCA CCACCTTCCC AGTCATCAAA AGATCCCCAA 
AATATGCCTC TCAAGCAATC TTTGAGATGC TGCCGCAATG ATCAGAAAAT CAATTCCTCT 
GAGAATTTTG AGGGTGTTGA AGAAACACCA GAAGAGGAGC TGGACGAAGT ACTTAAGCCG 
CAAAGAATCA ACCTAAAAAA TAGATTTGAC CTGATTGTCT ACCGAACACA TGAGGAGGCT 
TTGCAGATGT GCCAAGACCG CTGCCTTCCC GTGGCCTTTC CTTGCATGAT ACAAAACTGT 
GACTCTGTTG TCGTAAGCAT GCATAGTTTG AATCGACATT ACATTAATGT TCACAGGTTT 
AAACGCAAAC AGCTGACGGA AAATAAGGAC AAATTATGTT TCACTGCTGA ACAGTTGGAG 
GAGATCATAC AAAAGAAGTC TGCTGTGTCT GCTATACCTG ATTTAACAAG GATTCCAAAT 
GGCGTTCTCA AGATGGAGTA TCAGTCTGAG CCTGCCACTC CAGGTGGCCC GTCTCAACCA 
ATGAGCCTCC ATTCGATCAA AACAGATTCA AAGGAGAAAG AAGGGATTGA GTTTTCAGAA 
GAGCCGCTCC CTGACAGAAG TTTGCTCATT GAAGCTGAGG ATTTGCTCTA TGGCTCCCCA 
AAGTCAAGCG GGAACCCCGA GAAGCCTGTT TCTGAAGAGA GTCTCAACCA CAAAGATGAG 
CCAGAATCTG AATTAACAGC ACCTCCCCTC ATACGCCCCC CACCACTTGA CCTCTCTCCA 
CCTGCTACAC TTAGAATCAC AGTTGATGAA AGTTCATTTG AAGCTTCTGG TAAAGACAGT 
AAATCTATTA ATATTCCTTC TAGTGTTTCC ATATCGGTCC CCACCCCAAC TCGGCAGCCC 
CTCAGACGAA AGAATGAGCT CTCTGAACCA CCACTACCAC CAGTCCCTCT TTCTCCAGTC 
CCAATGGATC CTGTAGAGCA CAGTCTATCC CCAAGGGCTT TTGACATTGC AGCATACAAA 
CCTATGGGCT TCGAGTCATC ATTCTTGAAG TTCATTAAAG ACAAGGATGA GGAGATCAAA 
CTTGAAAAAC CATGGACTGC AGTAGTTTCT AATCCTTGCC TCAAGCCTGA TCCAAATCGC 
CGGCGAGATT GTTTCAGACG AAATTGCTCA GTCAAGGAGA ACACTGAAAG TGGAGCCCCC 
ATTTCTCGAA GCCGCAGGTT TCGGTCATCC CCTTTGAGGC ATCTGATTTC TAAAGGAGAG 
TGTACCTCTA TTCAGAATCT CCGTATGATT TTAGAGAGAG CTTTAGGGGG GTGTGGCGAC 
CAAGCTATAA AACAGCTACA GTTCCTGAAG CCTGTGGTGG TTCTAGAGAG GCCAAAGTCC 
TTTGCCTCCA TTTTCGATCT CTTGCCCTCT GAAACTAAAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rlf ( XM_685832.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_690924.6 Danio rerio rearranged L-myc fusion (rlf), mRNA.

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CloneID	ODa22526
Clone ID Related Accession (Same CDS sequence)	XM_685832.8 , XM_021467415.1
Accession Version	XM_685832.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein Rlf
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_685832.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCT GTGCGTACGC GCCGTGGCTG CCAGTGAACA TGGCGGATGA GAATGCTGAA 
GCGGAGCTCG ACTGGGGCGG CCGACACAGC CTGCTGGTCG ACGAGGACAC ATTTTTCGCC 
ATGGAGGGTT TGCAAGCAAC CCTTCAGCAA CACGAAGCCG AGCTTCGACA GCAAGACGTT 
TCTGAGGAAT CGTCCACGGG TTATTGCAAC AACTTCTGCG AGGCTCTAAT GCATTATGCT 
GGGAGCAGGA ATTCGTTGGA ACATGGATTG CCTCTATTGG AAGTTTACTG TCTTTCAATC 
AACTGTTTTG CTGCTGCCAG GCCACATCTC ACAGGCGACT CCCCCAGTGT TGCCCTTGTG 
CTTAAAAGAC TTGCATTGAG TTGCCTTGAA TTGTTGTTGT CAGTGCCTCA AAATAATATA 
CCTTTGGAAG CCTGGCTGCA GTTCCATGGC TCAGTTCAGG CGGCCCATGA AGCCATGCTG 
CAGTATGGAA GTACAGATTT GCATGCCCTG CGGAACATCA CTGGTGAAGG TGGAGCTTGG 
AACAATTCTG TCATGGTCTC GCTTCTCACC GGTCAGCCCA CAGACCCGGA TGAGGTAAAT 
GCCTATCTAG CTCTGGAGGG AGATGATTTC ATGGAAATGC GAATCAAACA TCTGGAGAAA 
GTAGGCGAGC TAGAGAAAGC ACTGATCCTC ATCAAAGCCT GTGCTAACTG TAGCCTTCTG 
TCTAACCAGT CAACATTTCG CCAAACCTTT GTAACCCAAC TTTGCCAGCT GCTGCCCAGT 
GAAGAAGCTA TTTTAGAGAT TTCTAGAATA GATGCAAAAG ATGTCCTGGA TATTATTTGC 
AACCTAGATG CTGAGGGCGA TGAGAGAACA GCCTTCATCT TATGCACAAC GTATTTTACA 
CAGCAGCTAC AGCAAGACAG CACATACTGT TCCTGGGAGT TAACCCTCCT TTGGAGTAAA 
CTGCAGAGAA GAATTGATGT GTCCCTTGAG TCATTTATCG AACGCTGTCT CCAGTTTGCA 
GCCATCGCCA GGACTATCTA CCATCTTCTT TTCCTTATTC GTGTAATTCA AACTGAGGCA 
ATGCAGCTTG GCCTTGCAGT ATCAGTTGAG CTGTGTGTGA AGGCTCTACG CCTTCCAAGG 
CAAGATGACA TTGAAGCAAA AACAATGGTC TGCAAAGCTG TTGTCTGTCT TCTCCCTGAT 
GATCTTGAGG TGATACGGGC TTGCCAGCTT ACAGAATTCC TCTTGAGCCC ATCCCAGGAA 
GCATTTGATA TCCTTGAGGA GCTCTATCTG CGTCCTGATG AAAAATATGA TGAGGAAAAC 
GCCATAATTC CAAATTTCCT GCGCTGTGAG CTGCTACTTT CTCTGAAAGC ACATTGGCCC 
ATTGACCCTG AATTCTGGGA CTGGAAAACT TTAAAACGCT ACTGCATTCA GTTGCTGGGG 
CTGGAACCTG TGGATGAAAT TGAAGATGAA GCAGCACCTG GGGAGCTATG TAATCTTGGA 
GTGGTTTTGA ATGAAGAGGC TGGGAAGGAG GAGGAAGACG AATGTGAGCT TGATGCGCTC 
AGCAGCGCAG GGGAGCAGCA AACTGTGGAA GAATCAGAAA AGGGACAAAA AGATTCAGAA 
GATGTCTCTA AGTTAGCTGA TAAGCATCAG AGGGATAAAT TTCTCTGCCA GATATGCCAT 
AAAGAAGTGG CAGAGACCCG GTTCTGTCAC CACGCCAGAA AACATATGGA GGATGGCATG 
TGGACATGCC CTGTGTGTCT ACAAAAGTTT AAAACTAAAA AGGAGTTTGA GCCACACTCC 
AAAAAACACA TTCAGATTTC TGCAAAAGAT CACTTTAAAA AGAAAAAAGT GAAGAAGAAG 
GTGGACCAGA CTAAAGATTT CTTCAAGGAG GATAGTTCTG ATGAGCTAGA GCCTGGTCAA 
ATTCCTTTAG ACCCTTCACT TGCCATGTAC TACCAGTCCA CCCATGATCC TGTAGTATTG 
GAACATATAT TAGAGAAAGC TGCCAGTGTG CCTGAAAAGC AAGTAGAAAG TGACTATGTT 
ACATTTGACT ACATCAACAC GTACTTTAAG TTACAGGATC GTGATGTTTA CCAGTGTCCA 
GCCACTGACT GCTCAAAGAA TTTTAAACTT TTCAAGTACC TGGGTGTGCA CATCAAAAAT 
GAGCATGATA GTGAAGACCC CAATTTGAAA CATTATCTTA AGATGAAAGA CTGTCGGGAG 
AAATGCACTT TCTGCCGGAA GACATTCATG ACCGCCTATC ACCATCGACT GCACCGTTGG 
ATTCATTATG GGGATGATCC TTACATGTGT GTGGTCACAG GTTGTGGTGC CCGCTTTGAC 
ACCACCAATG AACTTTTAGC ACATAAGCAT GGCCATGGGT TTCGATTGTC CTACTGTTGT 
GAGTTAAAAG GCTGCAGTTT CTCCTACTGT GACCTTGGGC AGCTTTACCA CCATGAGGCG 
CAGCACTTTC GTGATGCTGC TTACACTTGT ACCAGCCCGG GGTGCAAGAA CTTTTACTAT 
TCTCGCAAAG AATTTCTGCA ACACTTGGCC ACTCATGACA TCACTTTCAC AGAAAAAGAC 
TTTGAGGCTC AGAGAAAAGC AAAAAGGAAA CTCTTGCTCG CAGTTGTAGA AAGTGATTCG 
GCTTTTGGAT CAAAGGTTGA AACAGAGAAT TGTGTCCAGA AAAGTGGTTC AAGTTCATCT 
TCACAGACCT TGGCAAAGGG TCGATCATCA GTCTCGCAAA CATGTGTTGC TGTCTGCTTT 
GATGGTAAAA AGTTTACATG TGGCTTAAAA AAGTGTGGAA AAACATACAC TACTGCAAGA 
GAACTTCAGA AGCACTTGAG AATTGCTCAT TCAGACACAT TTAATGAGAA GGATCAGTTA 
TGCAAAAAGA AAGTACAAGT AAAGCATTCT ACGACTCGAA ATGAAGATTT ACCTAAAAGT 
GACCCGGATA ACACTGTAAA AACGTCATCT ACAGAAAAAT CTAGTCCCAA AAAGGCTCTC 
TCACCTGCGT CTGAGTCTGT AGATTGTGAG ACAACCAAAT CCAATTTGGA CCTTGATGCA 
GCACTAACCA ATATCATGCT TGGATTGGGC CAATTAAACC TTAATTCTTC CAACACCAGA 
CATTCTATGT GTAATTCACA GAGCACTTGT ACACCTACCT TGAATACAAG GTCTCCTGCC 
AAAGCAGCAC ACAGCAAAAA TGAGTCAAGA TCTCACGTCA CAGGTAGTGA ATCAAAAAAG 
CTGCCAAACA AGTCAGAATC ACCAAAAACA CATGACAATA ATAAAGATGT ATCTGATAAG 
AAGCAAAAAA AAGAATCTGT CTCAGAGGAG GAACAACCAC AAAGCAAGTC CTTAACCAAG 
CCTTATAACT GCGAAGCCAA AAGCTGCCAC TATCAGAGCA CTACCAGTTG TGCTTTAATG 
CAGCACTATA TTAAGGTTCA TGGTTACTCC GAGAAGATGG TGAAAGAGAT GGAGGTGTTT 
CAATCTGAGA CATTGGAGCC TTTCAAAACA CCTGACAGCT CTGAAAATTC GGTCTTGGAG 
GATGATCAAT CACAAAGCGA GTTTCTTGTG CAGGCTACAG TCAAGCCTTA TGCCTGTGAA 
GCGAAGAGTT GCAACTACCA GAGTGTTACT AGTCATGCTT TATTGAAGCA CTACATCAAG 
ATTCATGGGT ACTCCGAAAA GAAGGTGAAA AATATGGAGG TGTTTCAGTC TCAGATATTC 
AAGCCTTTTA AGTGCCATTT GTGCTCCAAG AGTTACAGAA ACAAAAAAGA ACTGAGGCGT 
CATTATATTC AAATGCATCA CATAAAAGAA GACATTGTTG AACAAATGAA GTGCTCTTTT 
AAAAAGAATG TAGTTGAAAA AGCACCAGTG TTGTCATCAC CTAAAAAAAA CACTGAGGTA 
AAGCCACAGT CTTCAAAGAA GGCTGATGTT AAAACCCAAA AACAAGACAA TGCCCTTTGG 
CAACAGAAAT GCCCAAAACA TGATTATCGG TTGTGGGTCA GAAAAGTAGT TACGAAAAAC 
GTGCATGTTA AAGCTGAAAA AAAAAAGAAA GCCTCTTCGG AAACTCGATC TGATGTAAAA 
AACACAACGG TTGATGTTCG GGGAAGCCGA CGCTTAATTT CAAAAGGGAA TCTGAGCTAC 
ATCCTCACTA AATACAATAA ACCCTTTCAC TGCGTGCACA AAAACTGCAA TGCAGCCTTC 
AATAACCAGA ATGCCCTTGT TGGCCACCTT CAGATGGTGC ATCATTATAA CCGATCACAG 
TTGTGCTTTC CATGTGAACA CAAAGGATGC GAGAAGCAGT ATAGCACCTT ATCTAGTCTT 
ACTAAACACT ACAGAAGAGA CCACAACTTC ACCAAAAAAG AGAAAGAAAG GAAAATTCTG 
CCGAAACTGC CCTGTACATC TAAAAGTATA CCAAAAAGAC AGATGGCCTC AACAGAGGAG 
CCCATACCAA GGTTCAAGTG TACCTACGCT AATTGCAATG AGTCCTACCA TCTCAACAGC 
AGCCTCTTGC GTCACACAAG TCAGTTTCAC CAAAACCAGA CCCCCCAGAA TTCGGCTAAT 
ACTTCAGGGA ACAACGAGTT TAAAACTAGT TATAAGAAGC ACGTGTTCTA TAGGCACTGT 
GATCCGTCTG ATTCCCTTGT GGTGCGTCTC CAGAGTACAC CCAAGAAAGA AGAGTCAAAA 
AATGGATGCC AAACGAAGTT AATCATCTCA CCACCTTCCC AGTCATCAAA AGATTCCCAA 
AATATGCCTC TCAAGCAATC TTTGAGATGC TGCCGCAATG ATCAGAAAAT CAATTCCTCT 
GAGAATTTTG AGGGTGTTGA AGAAACACCA GAAGAGGAGC TGGACGAAGT ACTTAAGCCG 
CAAAGAATCA ACCTAAAAAA TAGATTTGAC CTGATTGTCT ACCGAACACA TGAGGAGGCT 
TTGCAGATGT GCCAAGACCG CTGCCTTCCC GTGGCCTTTC CTTGCATGAT ACAAAACTGT 
GACTCTGTTG TCGTAAGCAT GCATAGTTTG AATCGACATT ACATTAATGT TCACAGGTTT 
AAACGCAAAC AGCTGACGGA AAATAAGGAC AAATTATGTT TCACTGCTGA ACAGTTGGAG 
GAGATCATAC AAAAGAAGTC TGCTGTGTCT GCTATACCTG ATTTAACAAG GATTCCAAAT 
GGCGTTCTCA AGATGGAGTA TCAGTCTGAG CCTGCCACTC CAGGTGGCCC GTCTCAACCA 
ATGAGCCTCC ATTCGATCAA AACAGATTCA AAGGAGAAAG AAGGGATTGA GTTTTCAGAA 
GAGCCGCTCC CTGACAGAAG TTTGCTCATT GAAGCTGAGG ATTTGCTCTA TGGCTCCCCA 
AAGTCAAGCG GGAACCCCGA GAAGCCTGTT TCTGAAGAGA GTCTCAACCA CAAAGATGAG 
CCAGAATCTG AATTAACAGC ACCTCCCCTC ATACGCCCCC CACCACTTGA CCTCTCTCCA 
CCTGCTACAC TTAGAATCAC AGTTGATGAA AGTTCATTTG AAGCTTCTGG TAAAGACAGT 
AAATCTATTA ATATTCCTTC TAGTGTTTCC ATATCGGTCC CCACCCCAAC TCGGCAGCCC 
CTCAGACGAA AGAATGAGCT CTCTGAACCA CCACTACCAC CAGTCCCTCT TTCTCCAGTC 
CCAATGGATC CTGTAGAGCA CAGTCTATCC CCAAGGGCTT TTGACATTGC AGCATACAAA 
CCTATGGGCT TCGAGTCATC ATTCTTGAAG TTCATTAAAG ACAAGGATGA GGAGATCAAA 
CTTGAAAAAC CATGGACTGC AGTAGTTTCT AATCCTTGCC TCAAGCCTGA TCCAAATCGC 
CGGCGAGATT GTTTCAGACG AAATTGCTCA GTCAAGGAGA ACACTGAAAG TGGAGCCCCC 
ATTTCTCGAA GCCGCAGGTT TCGGTCATCC CCTTTGAGGC ATCTGATTTC TAAAGGAGAG 
TGTACCTCTA TTCAGAATCT CCGTATGATT TTAGAGAGAG CTTTAGGGGG GTGTGGCGAC 
CAAGCTATAA AACAGCTACA GTTCCTGAAG CCTGTGGTGG TTCTAGAGAG GCCAAAGTCC 
TTTGCCTCCA TTTTCGATCT CTTGCCCTCT GAAACTAAAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690924.6
CDS	1..6105
Translation	MTACAYAPWLPVNMADENAEAELDWGGRHSLLVDEDTFFAMEGLQATLQQHEAELRQQDVSEESSTGYCNNFCEALMHYAGSRNSLEHGLPLLEVYCLSINCFAAARPHLTGDSPSVALVLKRLALSCLELLLSVPQNNIPLEAWLQFHGSVQAAHEAMLQYGSTDLHALRNITGEGGAWNNSVMVSLLTGQPTDPDEVNAYLALEGDDFMEMRIKHLEKVGELEKALILIKACANCSLLSNQSTFRQTFVTQLCQLLPSEEAILEISRIDAKDVLDIICNLDAEGDERTAFILCTTYFTQQLQQDSTYCSWELTLLWSKLQRRIDVSLESFIERCLQFAAIARTIYHLLFLIRVIQTEAMQLGLAVSVELCVKALRLPRQDDIEAKTMVCKAVVCLLPDDLEVIRACQLTEFLLSPSQEAFDILEELYLRPDEKYDEENAIIPNFLRCELLLSLKAHWPIDPEFWDWKTLKRYCIQLLGLEPVDEIEDEAAPGELCNLGVVLNEEAGKEEEDECELDALSSAGEQQTVEESEKGQKDSEDVSKLADKHQRDKFLCQICHKEVAETRFCHHARKHMEDGMWTCPVCLQKFKTKKEFEPHSKKHIQISAKDHFKKKKVKKKVDQTKDFFKEDSSDELEPGQIPLDPSLAMYYQSTHDPVVLEHILEKAASVPEKQVESDYVTFDYINTYFKLQDRDVYQCPATDCSKNFKLFKYLGVHIKNEHDSEDPNLKHYLKMKDCREKCTFCRKTFMTAYHHRLHRWIHYGDDPYMCVVTGCGARFDTTNELLAHKHGHGFRLSYCCELKGCSFSYCDLGQLYHHEAQHFRDAAYTCTSPGCKNFYYSRKEFLQHLATHDITFTEKDFEAQRKAKRKLLLAVVESDSAFGSKVETENCVQKSGSSSSSQTLAKGRSSVSQTCVAVCFDGKKFTCGLKKCGKTYTTARELQKHLRIAHSDTFNEKDQLCKKKVQVKHSTTRNEDLPKSDPDNTVKTSSTEKSSPKKALSPASESVDCETTKSNLDLDAALTNIMLGLGQLNLNSSNTRHSMCNSQSTCTPTLNTRSPAKAAHSKNESRSHVTGSESKKLPNKSESPKTHDNNKDVSDKKQKKESVSEEEQPQSKSLTKPYNCEAKSCHYQSTTSCALMQHYIKVHGYSEKMVKEMEVFQSETLEPFKTPDSSENSVLEDDQSQSEFLVQATVKPYACEAKSCNYQSVTSHALLKHYIKIHGYSEKKVKNMEVFQSQIFKPFKCHLCSKSYRNKKELRRHYIQMHHIKEDIVEQMKCSFKKNVVEKAPVLSSPKKNTEVKPQSSKKADVKTQKQDNALWQQKCPKHDYRLWVRKVVTKNVHVKAEKKKKASSETRSDVKNTTVDVRGSRRLISKGNLSYILTKYNKPFHCVHKNCNAAFNNQNALVGHLQMVHHYNRSQLCFPCEHKGCEKQYSTLSSLTKHYRRDHNFTKKEKERKILPKLPCTSKSIPKRQMASTEEPIPRFKCTYANCNESYHLNSSLLRHTSQFHQNQTPQNSANTSGNNEFKTSYKKHVFYRHCDPSDSLVVRLQSTPKKEESKNGCQTKLIISPPSQSSKDSQNMPLKQSLRCCRNDQKINSSENFEGVEETPEEELDEVLKPQRINLKNRFDLIVYRTHEEALQMCQDRCLPVAFPCMIQNCDSVVVSMHSLNRHYINVHRFKRKQLTENKDKLCFTAEQLEEIIQKKSAVSAIPDLTRIPNGVLKMEYQSEPATPGGPSQPMSLHSIKTDSKEKEGIEFSEEPLPDRSLLIEAEDLLYGSPKSSGNPEKPVSEESLNHKDEPESELTAPPLIRPPPLDLSPPATLRITVDESSFEASGKDSKSINIPSSVSISVPTPTRQPLRRKNELSEPPLPPVPLSPVPMDPVEHSLSPRAFDIAAYKPMGFESSFLKFIKDKDEEIKLEKPWTAVVSNPCLKPDPNRRRDCFRRNCSVKENTESGAPISRSRRFRSSPLRHLISKGECTSIQNLRMILERALGGCGDQAIKQLQFLKPVVVLERPKSFASIFDLLPSETKV

Target ORF information:

RefSeq Version	XM_685832.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio rearranged L-myc fusion (rlf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685832.8

ORF Insert Sequence:

ATGACCGCCT GTGCGTACGC GCCGTGGCTG CCAGTGAACA TGGCGGATGA GAATGCTGAA 
GCGGAGCTCG ACTGGGGCGG CCGACACAGC CTGCTGGTCG ACGAGGACAC ATTTTTCGCC 
ATGGAGGGTT TGCAAGCAAC CCTTCAGCAA CACGAAGCCG AGCTTCGACA GCAAGACGTT 
TCTGAGGAAT CGTCCACGGG TTATTGCAAC AACTTCTGCG AGGCTCTAAT GCATTATGCT 
GGGAGCAGGA ATTCGTTGGA ACATGGATTG CCTCTATTGG AAGTTTACTG TCTTTCAATC 
AACTGTTTTG CTGCTGCCAG GCCACATCTC ACAGGCGACT CCCCCAGTGT TGCCCTTGTG 
CTTAAAAGAC TTGCATTGAG TTGCCTTGAA TTGTTGTTGT CAGTGCCTCA AAATAATATA 
CCTTTGGAAG CCTGGCTGCA GTTCCATGGC TCAGTTCAGG CGGCCCATGA AGCCATGCTG 
CAGTATGGAA GTACAGATTT GCATGCCCTG CGGAACATCA CTGGTGAAGG TGGAGCTTGG 
AACAATTCTG TCATGGTCTC GCTTCTCACC GGTCAGCCCA CAGACCCGGA TGAGGTAAAT 
GCCTATCTAG CTCTGGAGGG AGATGATTTC ATGGAAATGC GAATCAAACA TCTGGAGAAA 
GTAGGCGAGC TAGAGAAAGC ACTGATCCTC ATCAAAGCCT GTGCTAACTG TAGCCTTCTG 
TCTAACCAGT CAACATTTCG CCAAACCTTT GTAACCCAAC TTTGCCAGCT GCTGCCCAGT 
GAAGAAGCTA TTTTAGAGAT TTCTAGAATA GATGCAAAAG ATGTCCTGGA TATTATTTGC 
AACCTAGATG CTGAGGGCGA TGAGAGAACA GCCTTCATCT TATGCACAAC GTATTTTACA 
CAGCAGCTAC AGCAAGACAG CACATACTGT TCCTGGGAGT TAACCCTCCT TTGGAGTAAA 
CTGCAGAGAA GAATTGATGT GTCCCTTGAG TCATTTATCG AACGCTGTCT CCAGTTTGCA 
GCCATCGCCA GGACTATCTA CCATCTTCTT TTCCTTATTC GTGTAATTCA AACTGAGGCA 
ATGCAGCTTG GCCTTGCAGT ATCAGTTGAG CTGTGTGTGA AGGCTCTACG CCTTCCAAGG 
CAAGATGACA TTGAAGCAAA AACAATGGTC TGCAAAGCTG TTGTCTGTCT TCTCCCTGAT 
GATCTTGAGG TGATACGGGC TTGCCAGCTT ACAGAATTCC TCTTGAGCCC ATCCCAGGAA 
GCATTTGATA TCCTTGAGGA GCTCTATCTG CGTCCTGATG AAAAATATGA TGAGGAAAAC 
GCCATAATTC CAAATTTCCT GCGCTGTGAG CTGCTACTTT CTCTGAAAGC ACATTGGCCC 
ATTGACCCTG AATTCTGGGA CTGGAAAACT TTAAAACGCT ACTGCATTCA GTTGCTGGGG 
CTGGAACCTG TGGATGAAAT TGAAGATGAA GCAGCACCTG GGGAGCTATG TAATCTTGGA 
GTGGTTTTGA ATGAAGAGGC TGGGAAGGAG GAGGAAGACG AATGTGAGCT TGATGCGCTC 
AGCAGCGCAG GGGAGCAGCA AACTGTGGAA GAATCAGAAA AGGGACAAAA AGATTCAGAA 
GATGTCTCTA AGTTAGCTGA TAAGCATCAG AGGGATAAAT TTCTCTGCCA GATATGCCAT 
AAAGAAGTGG CAGAGACCCG GTTCTGTCAC CACGCCAGAA AACATATGGA GGATGGCATG 
TGGACATGCC CTGTGTGTCT ACAAAAGTTT AAAACTAAAA AGGAGTTTGA GCCACACTCC 
AAAAAACACA TTCAGATTTC TGCAAAAGAT CACTTTAAAA AGAAAAAAGT GAAGAAGAAG 
GTGGACCAGA CTAAAGATTT CTTCAAGGAG GATAGTTCTG ATGAGCTAGA GCCTGGTCAA 
ATTCCTTTAG ACCCTTCACT TGCCATGTAC TACCAGTCCA CCCATGATCC TGTAGTATTG 
GAACATATAT TAGAGAAAGC TGCCAGTGTG CCTGAAAAGC AAGTAGAAAG TGACTATGTT 
ACATTTGACT ACATCAACAC GTACTTTAAG TTACAGGATC GTGATGTTTA CCAGTGTCCA 
GCCACTGACT GCTCAAAGAA TTTTAAACTT TTCAAGTACC TGGGTGTGCA CATCAAAAAT 
GAGCATGATA GTGAAGACCC CAATTTGAAA CATTATCTTA AGATGAAAGA CTGTCGGGAG 
AAATGCACTT TCTGCCGGAA GACATTCATG ACCGCCTATC ACCATCGACT GCACCGTTGG 
ATTCATTATG GGGATGATCC TTACATGTGT GTGGTCACAG GTTGTGGTGC CCGCTTTGAC 
ACCACCAATG AACTTTTAGC ACATAAGCAT GGCCATGGGT TTCGATTGTC CTACTGTTGT 
GAGTTAAAAG GCTGCAGTTT CTCCTACTGT GACCTTGGGC AGCTTTACCA CCATGAGGCG 
CAGCACTTTC GTGATGCTGC TTACACTTGT ACCAGCCCGG GGTGCAAGAA CTTTTACTAT 
TCTCGCAAAG AATTTCTGCA ACACTTGGCC ACTCATGACA TCACTTTCAC AGAAAAAGAC 
TTTGAGGCTC AGAGAAAAGC AAAAAGGAAA CTCTTGCTCG CAGTTGTAGA AAGTGATTCG 
GCTTTTGGAT CAAAGGTTGA AACAGAGAAT TGTGTCCAGA AAAGTGGTTC AAGTTCATCT 
TCACAGACCT TGGCAAAGGG TCGATCATCA GTCTCGCAAA CATGTGTTGC TGTCTGCTTT 
GATGGTAAAA AGTTTACATG TGGCTTAAAA AAGTGTGGAA AAACATACAC TACTGCAAGA 
GAACTTCAGA AGCACTTGAG AATTGCTCAT TCAGACACAT TTAATGAGAA GGATCAGTTA 
TGCAAAAAGA AAGTACAAGT AAAGCATTCT ACGACTCGAA ATGAAGATTT ACCTAAAAGT 
GACCCGGATA ACACTGTAAA AACGTCATCT ACAGAAAAAT CTAGTCCCAA AAAGGCTCTC 
TCACCTGCGT CTGAGTCTGT AGATTGTGAG ACAACCAAAT CCAATTTGGA CCTTGATGCA 
GCACTAACCA ATATCATGCT TGGATTGGGC CAATTAAACC TTAATTCTTC CAACACCAGA 
CATTCTATGT GTAATTCACA GAGCACTTGT ACACCTACCT TGAATACAAG GTCTCCTGCC 
AAAGCAGCAC ACAGCAAAAA TGAGTCAAGA TCTCACGTCA CAGGTAGTGA ATCAAAAAAG 
CTGCCAAACA AGTCAGAATC ACCAAAAACA CATGACAATA ATAAAGATGT ATCTGATAAG 
AAGCAAAAAA AAGAATCTGT CTCAGAGGAG GAACAACCAC AAAGCAAGTC CTTAACCAAG 
CCTTATAACT GCGAAGCCAA AAGCTGCCAC TATCAGAGCA CTACCAGTTG TGCTTTAATG 
CAGCACTATA TTAAGGTTCA TGGTTACTCC GAGAAGATGG TGAAAGAGAT GGAGGTGTTT 
CAATCTGAGA CATTGGAGCC TTTCAAAACA CCTGACAGCT CTGAAAATTC GGTCTTGGAG 
GATGATCAAT CACAAAGCGA GTTTCTTGTG CAGGCTACAG TCAAGCCTTA TGCCTGTGAA 
GCGAAGAGTT GCAACTACCA GAGTGTTACT AGTCATGCTT TATTGAAGCA CTACATCAAG 
ATTCATGGGT ACTCCGAAAA GAAGGTGAAA AATATGGAGG TGTTTCAGTC TCAGATATTC 
AAGCCTTTTA AGTGCCATTT GTGCTCCAAG AGTTACAGAA ACAAAAAAGA ACTGAGGCGT 
CATTATATTC AAATGCATCA CATAAAAGAA GACATTGTTG AACAAATGAA GTGCTCTTTT 
AAAAAGAATG TAGTTGAAAA AGCACCAGTG TTGTCATCAC CTAAAAAAAA CACTGAGGTA 
AAGCCACAGT CTTCAAAGAA GGCTGATGTT AAAACCCAAA AACAAGACAA TGCCCTTTGG 
CAACAGAAAT GCCCAAAACA TGATTATCGG TTGTGGGTCA GAAAAGTAGT TACGAAAAAC 
GTGCATGTTA AAGCTGAAAA AAAAAAGAAA GCCTCTTCGG AAACTCGATC TGATGTAAAA 
AACACAACGG TTGATGTTCG GGGAAGCCGA CGCTTAATTT CAAAAGGGAA TCTGAGCTAC 
ATCCTCACTA AATACAATAA ACCCTTTCAC TGCGTGCACA AAAACTGCAA TGCAGCCTTC 
AATAACCAGA ATGCCCTTGT TGGCCACCTT CAGATGGTGC ATCATTATAA CCGATCACAG 
TTGTGCTTTC CATGTGAACA CAAAGGATGC GAGAAGCAGT ATAGCACCTT ATCTAGTCTT 
ACTAAACACT ACAGAAGAGA CCACAACTTC ACCAAAAAAG AGAAAGAAAG GAAAATTCTG 
CCGAAACTGC CCTGTACATC TAAAAGTATA CCAAAAAGAC AGATGGCCTC AACAGAGGAG 
CCCATACCAA GGTTCAAGTG TACCTACGCT AATTGCAATG AGTCCTACCA TCTCAACAGC 
AGCCTCTTGC GTCACACAAG TCAGTTTCAC CAAAACCAGA CCCCCCAGAA TTCGGCTAAT 
ACTTCAGGGA ACAACGAGTT TAAAACTAGT TATAAGAAGC ACGTGTTCTA TAGGCACTGT 
GATCCGTCTG ATTCCCTTGT GGTGCGTCTC CAGAGTACAC CCAAGAAAGA AGAGTCAAAA 
AATGGATGCC AAACGAAGTT AATCATCTCA CCACCTTCCC AGTCATCAAA AGATTCCCAA 
AATATGCCTC TCAAGCAATC TTTGAGATGC TGCCGCAATG ATCAGAAAAT CAATTCCTCT 
GAGAATTTTG AGGGTGTTGA AGAAACACCA GAAGAGGAGC TGGACGAAGT ACTTAAGCCG 
CAAAGAATCA ACCTAAAAAA TAGATTTGAC CTGATTGTCT ACCGAACACA TGAGGAGGCT 
TTGCAGATGT GCCAAGACCG CTGCCTTCCC GTGGCCTTTC CTTGCATGAT ACAAAACTGT 
GACTCTGTTG TCGTAAGCAT GCATAGTTTG AATCGACATT ACATTAATGT TCACAGGTTT 
AAACGCAAAC AGCTGACGGA AAATAAGGAC AAATTATGTT TCACTGCTGA ACAGTTGGAG 
GAGATCATAC AAAAGAAGTC TGCTGTGTCT GCTATACCTG ATTTAACAAG GATTCCAAAT 
GGCGTTCTCA AGATGGAGTA TCAGTCTGAG CCTGCCACTC CAGGTGGCCC GTCTCAACCA 
ATGAGCCTCC ATTCGATCAA AACAGATTCA AAGGAGAAAG AAGGGATTGA GTTTTCAGAA 
GAGCCGCTCC CTGACAGAAG TTTGCTCATT GAAGCTGAGG ATTTGCTCTA TGGCTCCCCA 
AAGTCAAGCG GGAACCCCGA GAAGCCTGTT TCTGAAGAGA GTCTCAACCA CAAAGATGAG 
CCAGAATCTG AATTAACAGC ACCTCCCCTC ATACGCCCCC CACCACTTGA CCTCTCTCCA 
CCTGCTACAC TTAGAATCAC AGTTGATGAA AGTTCATTTG AAGCTTCTGG TAAAGACAGT 
AAATCTATTA ATATTCCTTC TAGTGTTTCC ATATCGGTCC CCACCCCAAC TCGGCAGCCC 
CTCAGACGAA AGAATGAGCT CTCTGAACCA CCACTACCAC CAGTCCCTCT TTCTCCAGTC 
CCAATGGATC CTGTAGAGCA CAGTCTATCC CCAAGGGCTT TTGACATTGC AGCATACAAA 
CCTATGGGCT TCGAGTCATC ATTCTTGAAG TTCATTAAAG ACAAGGATGA GGAGATCAAA 
CTTGAAAAAC CATGGACTGC AGTAGTTTCT AATCCTTGCC TCAAGCCTGA TCCAAATCGC 
CGGCGAGATT GTTTCAGACG AAATTGCTCA GTCAAGGAGA ACACTGAAAG TGGAGCCCCC 
ATTTCTCGAA GCCGCAGGTT TCGGTCATCC CCTTTGAGGC ATCTGATTTC TAAAGGAGAG 
TGTACCTCTA TTCAGAATCT CCGTATGATT TTAGAGAGAG CTTTAGGGGG GTGTGGCGAC 
CAAGCTATAA AACAGCTACA GTTCCTGAAG CCTGTGGTGG TTCTAGAGAG GCCAAAGTCC 
TTTGCCTCCA TTTTCGATCT CTTGCCCTCT GAAACTAAAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rlf ( XM_021467415.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323090.1 Danio rerio rearranged L-myc fusion (rlf), mRNA.

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CloneID	ODa22526
Clone ID Related Accession (Same CDS sequence)	XM_685832.8 , XM_021467415.1
Accession Version	XM_021467415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein Rlf
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCT GTGCGTACGC GCCGTGGCTG CCAGTGAACA TGGCGGATGA GAATGCTGAA 
GCGGAGCTCG ACTGGGGCGG CCGACACAGC CTGCTGGTCG ACGAGGACAC ATTTTTCGCC 
ATGGAGGGTT TGCAAGCAAC CCTTCAGCAA CACGAAGCCG AGCTTCGACA GCAAGACGTT 
TCTGAGGAAT CGTCCACGGG TTATTGCAAC AACTTCTGCG AGGCTCTAAT GCATTATGCT 
GGGAGCAGGA ATTCGTTGGA ACATGGATTG CCTCTATTGG AAGTTTACTG TCTTTCAATC 
AACTGTTTTG CTGCTGCCAG GCCACATCTC ACAGGCGACT CCCCCAGTGT TGCCCTTGTG 
CTTAAAAGAC TTGCATTGAG TTGCCTTGAA TTGTTGTTGT CAGTGCCTCA AAATAATATA 
CCTTTGGAAG CCTGGCTGCA GTTCCATGGC TCAGTTCAGG CGGCCCATGA AGCCATGCTG 
CAGTATGGAA GTACAGATTT GCATGCCCTG CGGAACATCA CTGGTGAAGG TGGAGCTTGG 
AACAATTCTG TCATGGTCTC GCTTCTCACC GGTCAGCCCA CAGACCCGGA TGAGGTAAAT 
GCCTATCTAG CTCTGGAGGG AGATGATTTC ATGGAAATGC GAATCAAACA TCTGGAGAAA 
GTAGGCGAGC TAGAGAAAGC ACTGATCCTC ATCAAAGCCT GTGCTAACTG TAGCCTTCTG 
TCTAACCAGT CAACATTTCG CCAAACCTTT GTAACCCAAC TTTGCCAGCT GCTGCCCAGT 
GAAGAAGCTA TTTTAGAGAT TTCTAGAATA GATGCAAAAG ATGTCCTGGA TATTATTTGC 
AACCTAGATG CTGAGGGCGA TGAGAGAACA GCCTTCATCT TATGCACAAC GTATTTTACA 
CAGCAGCTAC AGCAAGACAG CACATACTGT TCCTGGGAGT TAACCCTCCT TTGGAGTAAA 
CTGCAGAGAA GAATTGATGT GTCCCTTGAG TCATTTATCG AACGCTGTCT CCAGTTTGCA 
GCCATCGCCA GGACTATCTA CCATCTTCTT TTCCTTATTC GTGTAATTCA AACTGAGGCA 
ATGCAGCTTG GCCTTGCAGT ATCAGTTGAG CTGTGTGTGA AGGCTCTACG CCTTCCAAGG 
CAAGATGACA TTGAAGCAAA AACAATGGTC TGCAAAGCTG TTGTCTGTCT TCTCCCTGAT 
GATCTTGAGG TGATACGGGC TTGCCAGCTT ACAGAATTCC TCTTGAGCCC ATCCCAGGAA 
GCATTTGATA TCCTTGAGGA GCTCTATCTG CGTCCTGATG AAAAATATGA TGAGGAAAAC 
GCCATAATTC CAAATTTCCT GCGCTGTGAG CTGCTACTTT CTCTGAAAGC ACATTGGCCC 
ATTGACCCTG AATTCTGGGA CTGGAAAACT TTAAAACGCT ACTGCATTCA GTTGCTGGGG 
CTGGAACCTG TGGATGAAAT TGAAGATGAA GCAGCACCTG GGGAGCTATG TAATCTTGGA 
GTGGTTTTGA ATGAAGAGGC TGGGAAGGAG GAGGAAGACG AATGTGAGCT TGATGCGCTC 
AGCAGCGCAG GGGAGCAGCA AACTGTGGAA GAATCAGAAA AGGGACAAAA AGATTCAGAA 
GATGTCTCTA AGTTAGCTGA TAAGCATCAG AGGGATAAAT TTCTCTGCCA GATATGCCAT 
AAAGAAGTGG CAGAGACCCG GTTCTGTCAC CACGCCAGAA AACATATGGA GGATGGCATG 
TGGACATGCC CTGTGTGTCT ACAAAAGTTT AAAACTAAAA AGGAGTTTGA GCCACACTCC 
AAAAAACACA TTCAGATTTC TGCAAAAGAT CACTTTAAAA AGAAAAAAGT GAAGAAGAAG 
GTGGACCAGA CTAAAGATTT CTTCAAGGAG GATAGTTCTG ATGAGCTAGA GCCTGGTCAA 
ATTCCTTTAG ACCCTTCACT TGCCATGTAC TACCAGTCCA CCCATGATCC TGTAGTATTG 
GAACATATAT TAGAGAAAGC TGCCAGTGTG CCTGAAAAGC AAGTAGAAAG TGACTATGTT 
ACATTTGACT ACATCAACAC GTACTTTAAG TTACAGGATC GTGATGTTTA CCAGTGTCCA 
GCCACTGACT GCTCAAAGAA TTTTAAACTT TTCAAGTACC TGGGTGTGCA CATCAAAAAT 
GAGCATGATA GTGAAGACCC CAATTTGAAA CATTATCTTA AGATGAAAGA CTGTCGGGAG 
AAATGCACTT TCTGCCGGAA GACATTCATG ACCGCCTATC ACCATCGACT GCACCGTTGG 
ATTCATTATG GGGATGATCC TTACATGTGT GTGGTCACAG GTTGTGGTGC CCGCTTTGAC 
ACCACCAATG AACTTTTAGC ACATAAGCAT GGCCATGGGT TTCGATTGTC CTACTGTTGT 
GAGTTAAAAG GCTGCAGTTT CTCCTACTGT GACCTTGGGC AGCTTTACCA CCATGAGGCG 
CAGCACTTTC GTGATGCTGC TTACACTTGT ACCAGCCCGG GGTGCAAGAA CTTTTACTAT 
TCTCGCAAAG AATTTCTGCA ACACTTGGCC ACTCATGACA TCACTTTCAC AGAAAAAGAC 
TTTGAGGCTC AGAGAAAAGC AAAAAGGAAA CTCTTGCTCG CAGTTGTAGA AAGTGATTCG 
GCTTTTGGAT CAAAGGTTGA AACAGAGAAT TGTGTCCAGA AAAGTGGTTC AAGTTCATCT 
TCACAGACCT TGGCAAAGGG TCGATCATCA GTCTCGCAAA CATGTGTTGC TGTCTGCTTT 
GATGGTAAAA AGTTTACATG TGGCTTAAAA AAGTGTGGAA AAACATACAC TACTGCAAGA 
GAACTTCAGA AGCACTTGAG AATTGCTCAT TCAGACACAT TTAATGAGAA GGATCAGTTA 
TGCAAAAAGA AAGTACAAGT AAAGCATTCT ACGACTCGAA ATGAAGATTT ACCTAAAAGT 
GACCCGGATA ACACTGTAAA AACGTCATCT ACAGAAAAAT CTAGTCCCAA AAAGGCTCTC 
TCACCTGCGT CTGAGTCTGT AGATTGTGAG ACAACCAAAT CCAATTTGGA CCTTGATGCA 
GCACTAACCA ATATCATGCT TGGATTGGGC CAATTAAACC TTAATTCTTC CAACACCAGA 
CATTCTATGT GTAATTCACA GAGCACTTGT ACACCTACCT TGAATACAAG GTCTCCTGCC 
AAAGCAGCAC ACAGCAAAAA TGAGTCAAGA TCTCACGTCA CAGGTAGTGA ATCAAAAAAG 
CTGCCAAACA AGTCAGAATC ACCAAAAACA CATGACAATA ATAAAGATGT ATCTGATAAG 
AAGCAAAAAA AAGAATCTGT CTCAGAGGAG GAACAACCAC AAAGCAAGTC CTTAACCAAG 
CCTTATAACT GCGAAGCCAA AAGCTGCCAC TATCAGAGCA CTACCAGTTG TGCTTTAATG 
CAGCACTATA TTAAGGTTCA TGGTTACTCC GAGAAGATGG TGAAAGAGAT GGAGGTGTTT 
CAATCTGAGA CATTGGAGCC TTTCAAAACA CCTGACAGCT CTGAAAATTC GGTCTTGGAG 
GATGATCAAT CACAAAGCGA GTTTCTTGTG CAGGCTACAG TCAAGCCTTA TGCCTGTGAA 
GCGAAGAGTT GCAACTACCA GAGTGTTACT AGTCATGCTT TATTGAAGCA CTACATCAAG 
ATTCATGGGT ACTCCGAAAA GAAGGTGAAA AATATGGAGG TGTTTCAGTC TCAGATATTC 
AAGCCTTTTA AGTGCCATTT GTGCTCCAAG AGTTACAGAA ACAAAAAAGA ACTGAGGCGT 
CATTATATTC AAATGCATCA CATAAAAGAA GACATTGTTG AACAAATGAA GTGCTCTTTT 
AAAAAGAATG TAGTTGAAAA AGCACCAGTG TTGTCATCAC CTAAAAAAAA CACTGAGGTA 
AAGCCACAGT CTTCAAAGAA GGCTGATGTT AAAACCCAAA AACAAGACAA TGCCCTTTGG 
CAACAGAAAT GCCCAAAACA TGATTATCGG TTGTGGGTCA GAAAAGTAGT TACGAAAAAC 
GTGCATGTTA AAGCTGAAAA AAAAAAGAAA GCCTCTTCGG AAACTCGATC TGATGTAAAA 
AACACAACGG TTGATGTTCG GGGAAGCCGA CGCTTAATTT CAAAAGGGAA TCTGAGCTAC 
ATCCTCACTA AATACAATAA ACCCTTTCAC TGCGTGCACA AAAACTGCAA TGCAGCCTTC 
AATAACCAGA ATGCCCTTGT TGGCCACCTT CAGATGGTGC ATCATTATAA CCGATCACAG 
TTGTGCTTTC CATGTGAACA CAAAGGATGC GAGAAGCAGT ATAGCACCTT ATCTAGTCTT 
ACTAAACACT ACAGAAGAGA CCACAACTTC ACCAAAAAAG AGAAAGAAAG GAAAATTCTG 
CCGAAACTGC CCTGTACATC TAAAAGTATA CCAAAAAGAC AGATGGCCTC AACAGAGGAG 
CCCATACCAA GGTTCAAGTG TACCTACGCT AATTGCAATG AGTCCTACCA TCTCAACAGC 
AGCCTCTTGC GTCACACAAG TCAGTTTCAC CAAAACCAGA CCCCCCAGAA TTCGGCTAAT 
ACTTCAGGGA ACAACGAGTT TAAAACTAGT TATAAGAAGC ACGTGTTCTA TAGGCACTGT 
GATCCGTCTG ATTCCCTTGT GGTGCGTCTC CAGAGTACAC CCAAGAAAGA AGAGTCAAAA 
AATGGATGCC AAACGAAGTT AATCATCTCA CCACCTTCCC AGTCATCAAA AGATTCCCAA 
AATATGCCTC TCAAGCAATC TTTGAGATGC TGCCGCAATG ATCAGAAAAT CAATTCCTCT 
GAGAATTTTG AGGGTGTTGA AGAAACACCA GAAGAGGAGC TGGACGAAGT ACTTAAGCCG 
CAAAGAATCA ACCTAAAAAA TAGATTTGAC CTGATTGTCT ACCGAACACA TGAGGAGGCT 
TTGCAGATGT GCCAAGACCG CTGCCTTCCC GTGGCCTTTC CTTGCATGAT ACAAAACTGT 
GACTCTGTTG TCGTAAGCAT GCATAGTTTG AATCGACATT ACATTAATGT TCACAGGTTT 
AAACGCAAAC AGCTGACGGA AAATAAGGAC AAATTATGTT TCACTGCTGA ACAGTTGGAG 
GAGATCATAC AAAAGAAGTC TGCTGTGTCT GCTATACCTG ATTTAACAAG GATTCCAAAT 
GGCGTTCTCA AGATGGAGTA TCAGTCTGAG CCTGCCACTC CAGGTGGCCC GTCTCAACCA 
ATGAGCCTCC ATTCGATCAA AACAGATTCA AAGGAGAAAG AAGGGATTGA GTTTTCAGAA 
GAGCCGCTCC CTGACAGAAG TTTGCTCATT GAAGCTGAGG ATTTGCTCTA TGGCTCCCCA 
AAGTCAAGCG GGAACCCCGA GAAGCCTGTT TCTGAAGAGA GTCTCAACCA CAAAGATGAG 
CCAGAATCTG AATTAACAGC ACCTCCCCTC ATACGCCCCC CACCACTTGA CCTCTCTCCA 
CCTGCTACAC TTAGAATCAC AGTTGATGAA AGTTCATTTG AAGCTTCTGG TAAAGACAGT 
AAATCTATTA ATATTCCTTC TAGTGTTTCC ATATCGGTCC CCACCCCAAC TCGGCAGCCC 
CTCAGACGAA AGAATGAGCT CTCTGAACCA CCACTACCAC CAGTCCCTCT TTCTCCAGTC 
CCAATGGATC CTGTAGAGCA CAGTCTATCC CCAAGGGCTT TTGACATTGC AGCATACAAA 
CCTATGGGCT TCGAGTCATC ATTCTTGAAG TTCATTAAAG ACAAGGATGA GGAGATCAAA 
CTTGAAAAAC CATGGACTGC AGTAGTTTCT AATCCTTGCC TCAAGCCTGA TCCAAATCGC 
CGGCGAGATT GTTTCAGACG AAATTGCTCA GTCAAGGAGA ACACTGAAAG TGGAGCCCCC 
ATTTCTCGAA GCCGCAGGTT TCGGTCATCC CCTTTGAGGC ATCTGATTTC TAAAGGAGAG 
TGTACCTCTA TTCAGAATCT CCGTATGATT TTAGAGAGAG CTTTAGGGGG GTGTGGCGAC 
CAAGCTATAA AACAGCTACA GTTCCTGAAG CCTGTGGTGG TTCTAGAGAG GCCAAAGTCC 
TTTGCCTCCA TTTTCGATCT CTTGCCCTCT GAAACTAAAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323090.1
CDS	19..6123
Translation	MTACAYAPWLPVNMADENAEAELDWGGRHSLLVDEDTFFAMEGLQATLQQHEAELRQQDVSEESSTGYCNNFCEALMHYAGSRNSLEHGLPLLEVYCLSINCFAAARPHLTGDSPSVALVLKRLALSCLELLLSVPQNNIPLEAWLQFHGSVQAAHEAMLQYGSTDLHALRNITGEGGAWNNSVMVSLLTGQPTDPDEVNAYLALEGDDFMEMRIKHLEKVGELEKALILIKACANCSLLSNQSTFRQTFVTQLCQLLPSEEAILEISRIDAKDVLDIICNLDAEGDERTAFILCTTYFTQQLQQDSTYCSWELTLLWSKLQRRIDVSLESFIERCLQFAAIARTIYHLLFLIRVIQTEAMQLGLAVSVELCVKALRLPRQDDIEAKTMVCKAVVCLLPDDLEVIRACQLTEFLLSPSQEAFDILEELYLRPDEKYDEENAIIPNFLRCELLLSLKAHWPIDPEFWDWKTLKRYCIQLLGLEPVDEIEDEAAPGELCNLGVVLNEEAGKEEEDECELDALSSAGEQQTVEESEKGQKDSEDVSKLADKHQRDKFLCQICHKEVAETRFCHHARKHMEDGMWTCPVCLQKFKTKKEFEPHSKKHIQISAKDHFKKKKVKKKVDQTKDFFKEDSSDELEPGQIPLDPSLAMYYQSTHDPVVLEHILEKAASVPEKQVESDYVTFDYINTYFKLQDRDVYQCPATDCSKNFKLFKYLGVHIKNEHDSEDPNLKHYLKMKDCREKCTFCRKTFMTAYHHRLHRWIHYGDDPYMCVVTGCGARFDTTNELLAHKHGHGFRLSYCCELKGCSFSYCDLGQLYHHEAQHFRDAAYTCTSPGCKNFYYSRKEFLQHLATHDITFTEKDFEAQRKAKRKLLLAVVESDSAFGSKVETENCVQKSGSSSSSQTLAKGRSSVSQTCVAVCFDGKKFTCGLKKCGKTYTTARELQKHLRIAHSDTFNEKDQLCKKKVQVKHSTTRNEDLPKSDPDNTVKTSSTEKSSPKKALSPASESVDCETTKSNLDLDAALTNIMLGLGQLNLNSSNTRHSMCNSQSTCTPTLNTRSPAKAAHSKNESRSHVTGSESKKLPNKSESPKTHDNNKDVSDKKQKKESVSEEEQPQSKSLTKPYNCEAKSCHYQSTTSCALMQHYIKVHGYSEKMVKEMEVFQSETLEPFKTPDSSENSVLEDDQSQSEFLVQATVKPYACEAKSCNYQSVTSHALLKHYIKIHGYSEKKVKNMEVFQSQIFKPFKCHLCSKSYRNKKELRRHYIQMHHIKEDIVEQMKCSFKKNVVEKAPVLSSPKKNTEVKPQSSKKADVKTQKQDNALWQQKCPKHDYRLWVRKVVTKNVHVKAEKKKKASSETRSDVKNTTVDVRGSRRLISKGNLSYILTKYNKPFHCVHKNCNAAFNNQNALVGHLQMVHHYNRSQLCFPCEHKGCEKQYSTLSSLTKHYRRDHNFTKKEKERKILPKLPCTSKSIPKRQMASTEEPIPRFKCTYANCNESYHLNSSLLRHTSQFHQNQTPQNSANTSGNNEFKTSYKKHVFYRHCDPSDSLVVRLQSTPKKEESKNGCQTKLIISPPSQSSKDSQNMPLKQSLRCCRNDQKINSSENFEGVEETPEEELDEVLKPQRINLKNRFDLIVYRTHEEALQMCQDRCLPVAFPCMIQNCDSVVVSMHSLNRHYINVHRFKRKQLTENKDKLCFTAEQLEEIIQKKSAVSAIPDLTRIPNGVLKMEYQSEPATPGGPSQPMSLHSIKTDSKEKEGIEFSEEPLPDRSLLIEAEDLLYGSPKSSGNPEKPVSEESLNHKDEPESELTAPPLIRPPPLDLSPPATLRITVDESSFEASGKDSKSINIPSSVSISVPTPTRQPLRRKNELSEPPLPPVPLSPVPMDPVEHSLSPRAFDIAAYKPMGFESSFLKFIKDKDEEIKLEKPWTAVVSNPCLKPDPNRRRDCFRRNCSVKENTESGAPISRSRRFRSSPLRHLISKGECTSIQNLRMILERALGGCGDQAIKQLQFLKPVVVLERPKSFASIFDLLPSETKV

Target ORF information:

RefSeq Version	XM_021467415.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rearranged L-myc fusion (rlf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467415.1

ORF Insert Sequence:

ATGACCGCCT GTGCGTACGC GCCGTGGCTG CCAGTGAACA TGGCGGATGA GAATGCTGAA 
GCGGAGCTCG ACTGGGGCGG CCGACACAGC CTGCTGGTCG ACGAGGACAC ATTTTTCGCC 
ATGGAGGGTT TGCAAGCAAC CCTTCAGCAA CACGAAGCCG AGCTTCGACA GCAAGACGTT 
TCTGAGGAAT CGTCCACGGG TTATTGCAAC AACTTCTGCG AGGCTCTAAT GCATTATGCT 
GGGAGCAGGA ATTCGTTGGA ACATGGATTG CCTCTATTGG AAGTTTACTG TCTTTCAATC 
AACTGTTTTG CTGCTGCCAG GCCACATCTC ACAGGCGACT CCCCCAGTGT TGCCCTTGTG 
CTTAAAAGAC TTGCATTGAG TTGCCTTGAA TTGTTGTTGT CAGTGCCTCA AAATAATATA 
CCTTTGGAAG CCTGGCTGCA GTTCCATGGC TCAGTTCAGG CGGCCCATGA AGCCATGCTG 
CAGTATGGAA GTACAGATTT GCATGCCCTG CGGAACATCA CTGGTGAAGG TGGAGCTTGG 
AACAATTCTG TCATGGTCTC GCTTCTCACC GGTCAGCCCA CAGACCCGGA TGAGGTAAAT 
GCCTATCTAG CTCTGGAGGG AGATGATTTC ATGGAAATGC GAATCAAACA TCTGGAGAAA 
GTAGGCGAGC TAGAGAAAGC ACTGATCCTC ATCAAAGCCT GTGCTAACTG TAGCCTTCTG 
TCTAACCAGT CAACATTTCG CCAAACCTTT GTAACCCAAC TTTGCCAGCT GCTGCCCAGT 
GAAGAAGCTA TTTTAGAGAT TTCTAGAATA GATGCAAAAG ATGTCCTGGA TATTATTTGC 
AACCTAGATG CTGAGGGCGA TGAGAGAACA GCCTTCATCT TATGCACAAC GTATTTTACA 
CAGCAGCTAC AGCAAGACAG CACATACTGT TCCTGGGAGT TAACCCTCCT TTGGAGTAAA 
CTGCAGAGAA GAATTGATGT GTCCCTTGAG TCATTTATCG AACGCTGTCT CCAGTTTGCA 
GCCATCGCCA GGACTATCTA CCATCTTCTT TTCCTTATTC GTGTAATTCA AACTGAGGCA 
ATGCAGCTTG GCCTTGCAGT ATCAGTTGAG CTGTGTGTGA AGGCTCTACG CCTTCCAAGG 
CAAGATGACA TTGAAGCAAA AACAATGGTC TGCAAAGCTG TTGTCTGTCT TCTCCCTGAT 
GATCTTGAGG TGATACGGGC TTGCCAGCTT ACAGAATTCC TCTTGAGCCC ATCCCAGGAA 
GCATTTGATA TCCTTGAGGA GCTCTATCTG CGTCCTGATG AAAAATATGA TGAGGAAAAC 
GCCATAATTC CAAATTTCCT GCGCTGTGAG CTGCTACTTT CTCTGAAAGC ACATTGGCCC 
ATTGACCCTG AATTCTGGGA CTGGAAAACT TTAAAACGCT ACTGCATTCA GTTGCTGGGG 
CTGGAACCTG TGGATGAAAT TGAAGATGAA GCAGCACCTG GGGAGCTATG TAATCTTGGA 
GTGGTTTTGA ATGAAGAGGC TGGGAAGGAG GAGGAAGACG AATGTGAGCT TGATGCGCTC 
AGCAGCGCAG GGGAGCAGCA AACTGTGGAA GAATCAGAAA AGGGACAAAA AGATTCAGAA 
GATGTCTCTA AGTTAGCTGA TAAGCATCAG AGGGATAAAT TTCTCTGCCA GATATGCCAT 
AAAGAAGTGG CAGAGACCCG GTTCTGTCAC CACGCCAGAA AACATATGGA GGATGGCATG 
TGGACATGCC CTGTGTGTCT ACAAAAGTTT AAAACTAAAA AGGAGTTTGA GCCACACTCC 
AAAAAACACA TTCAGATTTC TGCAAAAGAT CACTTTAAAA AGAAAAAAGT GAAGAAGAAG 
GTGGACCAGA CTAAAGATTT CTTCAAGGAG GATAGTTCTG ATGAGCTAGA GCCTGGTCAA 
ATTCCTTTAG ACCCTTCACT TGCCATGTAC TACCAGTCCA CCCATGATCC TGTAGTATTG 
GAACATATAT TAGAGAAAGC TGCCAGTGTG CCTGAAAAGC AAGTAGAAAG TGACTATGTT 
ACATTTGACT ACATCAACAC GTACTTTAAG TTACAGGATC GTGATGTTTA CCAGTGTCCA 
GCCACTGACT GCTCAAAGAA TTTTAAACTT TTCAAGTACC TGGGTGTGCA CATCAAAAAT 
GAGCATGATA GTGAAGACCC CAATTTGAAA CATTATCTTA AGATGAAAGA CTGTCGGGAG 
AAATGCACTT TCTGCCGGAA GACATTCATG ACCGCCTATC ACCATCGACT GCACCGTTGG 
ATTCATTATG GGGATGATCC TTACATGTGT GTGGTCACAG GTTGTGGTGC CCGCTTTGAC 
ACCACCAATG AACTTTTAGC ACATAAGCAT GGCCATGGGT TTCGATTGTC CTACTGTTGT 
GAGTTAAAAG GCTGCAGTTT CTCCTACTGT GACCTTGGGC AGCTTTACCA CCATGAGGCG 
CAGCACTTTC GTGATGCTGC TTACACTTGT ACCAGCCCGG GGTGCAAGAA CTTTTACTAT 
TCTCGCAAAG AATTTCTGCA ACACTTGGCC ACTCATGACA TCACTTTCAC AGAAAAAGAC 
TTTGAGGCTC AGAGAAAAGC AAAAAGGAAA CTCTTGCTCG CAGTTGTAGA AAGTGATTCG 
GCTTTTGGAT CAAAGGTTGA AACAGAGAAT TGTGTCCAGA AAAGTGGTTC AAGTTCATCT 
TCACAGACCT TGGCAAAGGG TCGATCATCA GTCTCGCAAA CATGTGTTGC TGTCTGCTTT 
GATGGTAAAA AGTTTACATG TGGCTTAAAA AAGTGTGGAA AAACATACAC TACTGCAAGA 
GAACTTCAGA AGCACTTGAG AATTGCTCAT TCAGACACAT TTAATGAGAA GGATCAGTTA 
TGCAAAAAGA AAGTACAAGT AAAGCATTCT ACGACTCGAA ATGAAGATTT ACCTAAAAGT 
GACCCGGATA ACACTGTAAA AACGTCATCT ACAGAAAAAT CTAGTCCCAA AAAGGCTCTC 
TCACCTGCGT CTGAGTCTGT AGATTGTGAG ACAACCAAAT CCAATTTGGA CCTTGATGCA 
GCACTAACCA ATATCATGCT TGGATTGGGC CAATTAAACC TTAATTCTTC CAACACCAGA 
CATTCTATGT GTAATTCACA GAGCACTTGT ACACCTACCT TGAATACAAG GTCTCCTGCC 
AAAGCAGCAC ACAGCAAAAA TGAGTCAAGA TCTCACGTCA CAGGTAGTGA ATCAAAAAAG 
CTGCCAAACA AGTCAGAATC ACCAAAAACA CATGACAATA ATAAAGATGT ATCTGATAAG 
AAGCAAAAAA AAGAATCTGT CTCAGAGGAG GAACAACCAC AAAGCAAGTC CTTAACCAAG 
CCTTATAACT GCGAAGCCAA AAGCTGCCAC TATCAGAGCA CTACCAGTTG TGCTTTAATG 
CAGCACTATA TTAAGGTTCA TGGTTACTCC GAGAAGATGG TGAAAGAGAT GGAGGTGTTT 
CAATCTGAGA CATTGGAGCC TTTCAAAACA CCTGACAGCT CTGAAAATTC GGTCTTGGAG 
GATGATCAAT CACAAAGCGA GTTTCTTGTG CAGGCTACAG TCAAGCCTTA TGCCTGTGAA 
GCGAAGAGTT GCAACTACCA GAGTGTTACT AGTCATGCTT TATTGAAGCA CTACATCAAG 
ATTCATGGGT ACTCCGAAAA GAAGGTGAAA AATATGGAGG TGTTTCAGTC TCAGATATTC 
AAGCCTTTTA AGTGCCATTT GTGCTCCAAG AGTTACAGAA ACAAAAAAGA ACTGAGGCGT 
CATTATATTC AAATGCATCA CATAAAAGAA GACATTGTTG AACAAATGAA GTGCTCTTTT 
AAAAAGAATG TAGTTGAAAA AGCACCAGTG TTGTCATCAC CTAAAAAAAA CACTGAGGTA 
AAGCCACAGT CTTCAAAGAA GGCTGATGTT AAAACCCAAA AACAAGACAA TGCCCTTTGG 
CAACAGAAAT GCCCAAAACA TGATTATCGG TTGTGGGTCA GAAAAGTAGT TACGAAAAAC 
GTGCATGTTA AAGCTGAAAA AAAAAAGAAA GCCTCTTCGG AAACTCGATC TGATGTAAAA 
AACACAACGG TTGATGTTCG GGGAAGCCGA CGCTTAATTT CAAAAGGGAA TCTGAGCTAC 
ATCCTCACTA AATACAATAA ACCCTTTCAC TGCGTGCACA AAAACTGCAA TGCAGCCTTC 
AATAACCAGA ATGCCCTTGT TGGCCACCTT CAGATGGTGC ATCATTATAA CCGATCACAG 
TTGTGCTTTC CATGTGAACA CAAAGGATGC GAGAAGCAGT ATAGCACCTT ATCTAGTCTT 
ACTAAACACT ACAGAAGAGA CCACAACTTC ACCAAAAAAG AGAAAGAAAG GAAAATTCTG 
CCGAAACTGC CCTGTACATC TAAAAGTATA CCAAAAAGAC AGATGGCCTC AACAGAGGAG 
CCCATACCAA GGTTCAAGTG TACCTACGCT AATTGCAATG AGTCCTACCA TCTCAACAGC 
AGCCTCTTGC GTCACACAAG TCAGTTTCAC CAAAACCAGA CCCCCCAGAA TTCGGCTAAT 
ACTTCAGGGA ACAACGAGTT TAAAACTAGT TATAAGAAGC ACGTGTTCTA TAGGCACTGT 
GATCCGTCTG ATTCCCTTGT GGTGCGTCTC CAGAGTACAC CCAAGAAAGA AGAGTCAAAA 
AATGGATGCC AAACGAAGTT AATCATCTCA CCACCTTCCC AGTCATCAAA AGATTCCCAA 
AATATGCCTC TCAAGCAATC TTTGAGATGC TGCCGCAATG ATCAGAAAAT CAATTCCTCT 
GAGAATTTTG AGGGTGTTGA AGAAACACCA GAAGAGGAGC TGGACGAAGT ACTTAAGCCG 
CAAAGAATCA ACCTAAAAAA TAGATTTGAC CTGATTGTCT ACCGAACACA TGAGGAGGCT 
TTGCAGATGT GCCAAGACCG CTGCCTTCCC GTGGCCTTTC CTTGCATGAT ACAAAACTGT 
GACTCTGTTG TCGTAAGCAT GCATAGTTTG AATCGACATT ACATTAATGT TCACAGGTTT 
AAACGCAAAC AGCTGACGGA AAATAAGGAC AAATTATGTT TCACTGCTGA ACAGTTGGAG 
GAGATCATAC AAAAGAAGTC TGCTGTGTCT GCTATACCTG ATTTAACAAG GATTCCAAAT 
GGCGTTCTCA AGATGGAGTA TCAGTCTGAG CCTGCCACTC CAGGTGGCCC GTCTCAACCA 
ATGAGCCTCC ATTCGATCAA AACAGATTCA AAGGAGAAAG AAGGGATTGA GTTTTCAGAA 
GAGCCGCTCC CTGACAGAAG TTTGCTCATT GAAGCTGAGG ATTTGCTCTA TGGCTCCCCA 
AAGTCAAGCG GGAACCCCGA GAAGCCTGTT TCTGAAGAGA GTCTCAACCA CAAAGATGAG 
CCAGAATCTG AATTAACAGC ACCTCCCCTC ATACGCCCCC CACCACTTGA CCTCTCTCCA 
CCTGCTACAC TTAGAATCAC AGTTGATGAA AGTTCATTTG AAGCTTCTGG TAAAGACAGT 
AAATCTATTA ATATTCCTTC TAGTGTTTCC ATATCGGTCC CCACCCCAAC TCGGCAGCCC 
CTCAGACGAA AGAATGAGCT CTCTGAACCA CCACTACCAC CAGTCCCTCT TTCTCCAGTC 
CCAATGGATC CTGTAGAGCA CAGTCTATCC CCAAGGGCTT TTGACATTGC AGCATACAAA 
CCTATGGGCT TCGAGTCATC ATTCTTGAAG TTCATTAAAG ACAAGGATGA GGAGATCAAA 
CTTGAAAAAC CATGGACTGC AGTAGTTTCT AATCCTTGCC TCAAGCCTGA TCCAAATCGC 
CGGCGAGATT GTTTCAGACG AAATTGCTCA GTCAAGGAGA ACACTGAAAG TGGAGCCCCC 
ATTTCTCGAA GCCGCAGGTT TCGGTCATCC CCTTTGAGGC ATCTGATTTC TAAAGGAGAG 
TGTACCTCTA TTCAGAATCT CCGTATGATT TTAGAGAGAG CTTTAGGGGG GTGTGGCGAC 
CAAGCTATAA AACAGCTACA GTTCCTGAAG CCTGTGGTGG TTCTAGAGAG GCCAAAGTCC 
TTTGCCTCCA TTTTCGATCT CTTGCCCTCT GAAACTAAAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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rlf cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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