pi4k2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pi4k2b
Entrez Gene ID	564750
Full Name	phosphatidylinositol 4-kinase type 2 beta
Synonyms	zgc:158305
General protein information	Preferred Names phosphatidylinositol 4-kinase type 2 beta Names phosphatidylinositol 4-kinase type 2-beta PI4KII-beta phosphatidylinositol 4-kinase II beta phosphatidylinositol 4-kinase type II-beta pi4kII beta
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045485.2	NP_001038950.1	phosphatidylinositol 4-kinase type 2-beta

Pathways from BioSystems

Homologies

Homo sapiens (human)	PI4K2B	NP_060793.2
Gallus gallus (chicken)	PI4K2B	NP_001026328.1
Danio rerio (zebrafish)	pi4k2b	NP_001038950.1
Saccharomyces cerevisiae (baker's yeast)	LSB6	NP_012435.1
Eremothecium gossypii	AGOS_AFR087W	NP_985634.2
Kluyveromyces lactis	KLLA0F14080g	XP_455712.1
Neurospora crassa	NCU04355	XP_955827.1
Xenopus tropicalis (tropical clawed frog)	pi4k2b	NP_001016953.1
	LOC693561	XP_002808453.1
Bos taurus (cattle)	PI4K2B	NP_001095534.1
Mus musculus (house mouse)	Pi4k2b	NP_080227.2
Rattus norvegicus (Norway rat)	Pi4k2b	NP_001005883.1
Magnaporthe oryzae (rice blast fungus)	MGG_07151	XP_003715349.1
Pan troglodytes (chimpanzee)	PI4K2B	XP_003310311.1
Canis lupus familiaris (dog)	PI4K2B	XP_005618711.1
Schizosaccharomyces pombe (fission yeast)	lsb6	XP_001713056.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

PI4K2?/AP-1-based TGN-endosomal sorting regulates Wnt signaling.
Wieffer M, Cibri?n Uhalte E, Posor Y, Otten C, Branz K, Sch?tz I, M?ssinger J, Schu P, Abdelilah-Seyfried S, Krau? M, Haucke V
Current biology : CB23(21)2185-90(2013 Nov)

Crucial role of phosphatidylinositol 4-kinase IIIalpha in development of zebrafish pectoral fin is linked to phosphoinositide 3-kinase and FGF signaling.
Ma H, Blake T, Chitnis A, Liu P, Balla T
Journal of cell science122(Pt 23)4303-10(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PI4-kinase type 2beta (PI4K2beta) is identified as a regulator of the trans-Golgi network (TGN)/endosomal sorting and Wnt signaling. PI4K2beta and AP-1 interact directly and are required for efficient sorting between endosomes and the TGN.
Title: PI4K2?/AP-1-based TGN-endosomal sorting regulates Wnt signaling.

The following pi4k2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pi4k2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01014	NM_001045485.2 Latest version!	Danio rerio phosphatidylinositol 4-kinase type 2 beta (pi4k2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pi4k2b ( NM_001045485.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038950.1 Danio rerio phosphatidylinositol 4-kinase type 2 beta (pi4k2b), mRNA.

Price & Availability↑

CloneID	ODa01014
Clone ID Related Accession (Same CDS sequence)	NM_001045485.2
Accession Version	NM_001045485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 4-kinase type 2-beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC129195.1. On Jan 10, 2007 this sequence version replaced NM_001045485.1. ##Evidence-Data-START## Transcript exon combination :: BC129195.1, AY929291.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATGGCAG AATGCGACCC CACCGACGGC GAGCCGGGGA ACGGCAGCGG AGATTCAACA 
CCGGAGACGA ACTTTCTCTC TTCGGAGGTG CCGGCTGTGT TATTTGAAAA AACCCGGTCG 
GCACCGTCGT TGGGACTCTC GTTGAACCCC GGAGCAGCTG TCCGGATTTC GGACTCGACG 
GAAAGCGTGT TGACGGAGCT GGAAGCTGAC GGGTCCGGCG AAGAGGCGCT CCTGTTACCG 
GGACCAGCCG GGAGCCTCTC ACCTCGAGCT GGAAAAGAGA AAAGGACACG GAGGAACATG 
CTCAGCTCAT CATCCGATAA TCTAGCATCT CCAGGTAATA GCAGTGGAGA ATTCAACCAT 
TTCCCAGAAG ACCCAGAGTT CGGGGAGATT ATCCAGAGGG CCGAACAGGC CATAGAAAAC 
GGCGTCTTTC CTGAACGCAT CTCACAAGGA TCTAGTGGAA GTTACTTTGT CAAGGATCCT 
AAAGGGAAAA TCATTGGGGT ATTCAAACCA AAATCAGAGG AGCCTTATGG TCACTTAAAC 
CCAAAATGGA CCAAATACTT CCATAAGGTT TGCTGCCCGT GCTGTTTTGG CCGTGGCTGC 
CTCATTCCCA ATCAGGGCTA CCTCTCTGAG GCAGCTGCCT CACTTGTGGA CCAAAAACTG 
GGTTTGTGGA TTGTACCCAA AACTAAGGTT GTACATTTGG CTAGCGAGAC TTTTCACTAT 
AATGCAATTG ACCGTGCCAA ATCCCGTGGT AAGAAATATG CACTTGAGAA AGTCCCCAAA 
GTTGGACGAC GGTTTCACAG AGTTGGCCTT CCTCCAAAGG TGGGCTCATT TCAGCTGTTT 
GTGGAGGGCT ATCATGAAGC TGACTTCTGG CTGAGGAAGT TTGAAGCAGA GCCTCTTCCA 
GAGAACATGA GGAAACAGCT GCAGTCTCAG TTTGAGAGGC TGGTTGTTTT GGATTACGTT 
ATTAGAAACA CAGACAGAGG GAATGATAAC TGGTTGATCA AGTATGAGAA GCCGGGCGAT 
GGAGAGCTGA CAGAGAAGGA ATCTGAGTGG ACTGATCCAA AAGACTCGGC CATAAAAATT 
GCAGCTATAG ATAATGGTTT GGCCTTCCCC TTTAAACATC CTGATGAGTG GAGAGCATAT 
CCGTTCCACT GGGCTTGGTT GCCTCAGGCC AAAGTGGCCT TTTCCCAGGA GACCCGAGAC 
CTTGTGCTAT CTCGCATTTC CGATATGAAC TTTGTACAGG ACCTCTGTGA GGACCTTTAT 
GAGATGTTCA GGACGGATAA AGGATTCGAC AAAACCATGT TTGAGAAACA GATGTCCGTT 
ATGAGAGGAC AGATCTTAAA CCTGACACAG GCTTTGAAAG ATGGGAAGAG CCCGATCCAG 
CTAGTGCAGA TGCCTCGTGT GGTGGTGGAG CGGAGCCGCA GTGGGGGCCA GGGCCGCGTG 
GTTCAGCTAG GTAACGCTTT CACTCAGACA TTTCACTGCA AGAGACCCTT CTTCACCTCC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038950.1
CDS	164..1669
Translation	MMAECDPTDGEPGNGSGDSTPETNFLSSEVPAVLFEKTRSAPSLGLSLNPGAAVRISDSTESVLTELEADGSGEEALLLPGPAGSLSPRAGKEKRTRRNMLSSSSDNLASPGNSSGEFNHFPEDPEFGEIIQRAEQAIENGVFPERISQGSSGSYFVKDPKGKIIGVFKPKSEEPYGHLNPKWTKYFHKVCCPCCFGRGCLIPNQGYLSEAAASLVDQKLGLWIVPKTKVVHLASETFHYNAIDRAKSRGKKYALEKVPKVGRRFHRVGLPPKVGSFQLFVEGYHEADFWLRKFEAEPLPENMRKQLQSQFERLVVLDYVIRNTDRGNDNWLIKYEKPGDGELTEKESEWTDPKDSAIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVAFSQETRDLVLSRISDMNFVQDLCEDLYEMFRTDKGFDKTMFEKQMSVMRGQILNLTQALKDGKSPIQLVQMPRVVVERSRSGGQGRVVQLGNAFTQTFHCKRPFFTSW

Target ORF information:

RefSeq Version	NM_001045485.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol 4-kinase type 2 beta (pi4k2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045485.2

ORF Insert Sequence:

ATGATGGCAG AATGCGACCC CACCGACGGC GAGCCGGGGA ACGGCAGCGG AGATTCAACA 
CCGGAGACGA ACTTTCTCTC TTCGGAGGTG CCGGCTGTGT TATTTGAAAA AACCCGGTCG 
GCACCGTCGT TGGGACTCTC GTTGAACCCC GGAGCAGCTG TCCGGATTTC GGACTCGACG 
GAAAGCGTGT TGACGGAGCT GGAAGCTGAC GGGTCCGGCG AAGAGGCGCT CCTGTTACCG 
GGACCAGCCG GGAGCCTCTC ACCTCGAGCT GGAAAAGAGA AAAGGACACG GAGGAACATG 
CTCAGCTCAT CATCCGATAA TCTAGCATCT CCAGGTAATA GCAGTGGAGA ATTCAACCAT 
TTCCCAGAAG ACCCAGAGTT CGGGGAGATT ATCCAGAGGG CCGAACAGGC CATAGAAAAC 
GGCGTCTTTC CTGAACGCAT CTCACAAGGA TCTAGTGGAA GTTACTTTGT CAAGGATCCT 
AAAGGGAAAA TCATTGGGGT ATTCAAACCA AAATCAGAGG AGCCTTATGG TCACTTAAAC 
CCAAAATGGA CCAAATACTT CCATAAGGTT TGCTGCCCGT GCTGTTTTGG CCGTGGCTGC 
CTCATTCCCA ATCAGGGCTA CCTCTCTGAG GCAGCTGCCT CACTTGTGGA CCAAAAACTG 
GGTTTGTGGA TTGTACCCAA AACTAAGGTT GTACATTTGG CTAGCGAGAC TTTTCACTAT 
AATGCAATTG ACCGTGCCAA ATCCCGTGGT AAGAAATATG CACTTGAGAA AGTCCCCAAA 
GTTGGACGAC GGTTTCACAG AGTTGGCCTT CCTCCAAAGG TGGGCTCATT TCAGCTGTTT 
GTGGAGGGCT ATCATGAAGC TGACTTCTGG CTGAGGAAGT TTGAAGCAGA GCCTCTTCCA 
GAGAACATGA GGAAACAGCT GCAGTCTCAG TTTGAGAGGC TGGTTGTTTT GGATTACGTT 
ATTAGAAACA CAGACAGAGG GAATGATAAC TGGTTGATCA AGTATGAGAA GCCGGGCGAT 
GGAGAGCTGA CAGAGAAGGA ATCTGAGTGG ACTGATCCAA AAGACTCGGC CATAAAAATT 
GCAGCTATAG ATAATGGTTT GGCCTTCCCC TTTAAACATC CTGATGAGTG GAGAGCATAT 
CCGTTCCACT GGGCTTGGTT GCCTCAGGCC AAAGTGGCCT TTTCCCAGGA GACCCGAGAC 
CTTGTGCTAT CTCGCATTTC CGATATGAAC TTTGTACAGG ACCTCTGTGA GGACCTTTAT 
GAGATGTTCA GGACGGATAA AGGATTCGAC AAAACCATGT TTGAGAAACA GATGTCCGTT 
ATGAGAGGAC AGATCTTAAA CCTGACACAG GCTTTGAAAG ATGGGAAGAG CCCGATCCAG 
CTAGTGCAGA TGCCTCGTGT GGTGGTGGAG CGGAGCCGCA GTGGGGGCCA GGGCCGCGTG 
GTTCAGCTAG GTAACGCTTT CACTCAGACA TTTCACTGCA AGAGACCCTT CTTCACCTCC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

PI4K2?/AP-1-based TGN-endosomal sorting regulates Wnt signaling.
Current biology : CB23(21)2185-90(2013 Nov)
Wieffer M,Cibri?n Uhalte E,Posor Y,Otten C,Branz K,Sch?tz I,M?ssinger J,Schu P,Abdelilah-Seyfried S,Krau? M,Haucke V

Crucial role of phosphatidylinositol 4-kinase IIIalpha in development of zebrafish pectoral fin is linked to phosphoinositide 3-kinase and FGF signaling.
Journal of cell science122(Pt 23)4303-10(2009 Dec)
Ma H,Blake T,Chitnis A,Liu P,Balla T

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pi4k2b cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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