ntn2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ntn2
Entrez Gene ID	641469
Full Name	netrin 2
General protein information	Preferred Names netrin 2 Names netrin-3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037415.1	NP_001032492.1	netrin-3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NTN3	NP_001264534.1
Danio rerio (zebrafish)	ntn2	NP_001032492.1
Xenopus tropicalis (tropical clawed frog)	ntn3	XP_004918062.1

Gene Ontology
Bibliography

Related articles in PubMed

Tris(1,3-dichloro-2-propyl) phosphate disrupts axonal growth, cholinergic system and motor behavior in early life zebrafish.
Cheng R, Jia Y, Dai L, Liu C, Wang J, Li G, Yu L
Aquatic toxicology (Amsterdam, Netherlands)1927-15(2017 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A Floor-Plate Extracellular Protein-Protein Interaction Screen Identifies Draxin as a Secreted Netrin-1 Antagonist.
Gao X, Metzger U, Panza P, Mahalwar P, Alsheimer S, Geiger H, Maischein HM, Levesque MP, Templin M, S?llner C
Cell reports12(4)694-708(2015 Jul)

Netrin signaling breaks the equivalence between two identified zebrafish motoneurons revealing a new role of intermediate targets.
Hale LA, Fowler DK, Eisen JS
PloS one6(10)e25841(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Netrin 2 transcript is first detected in the notochord and in developing somites at early somitogenesis
Title: Identification of new netrin family members in zebrafish: developmental expression of netrin 2 and netrin 4.

The following ntn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ntn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33744	NM_001037415.1 Latest version!	Danio rerio netrin 2 (ntn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ntn2 ( NM_001037415.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032492.1 Danio rerio netrin 2 (ntn2), mRNA.

Price & Availability↑

CloneID	ODa33744
Clone ID Related Accession (Same CDS sequence)	NM_001037415.1
Accession Version	NM_001037415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Danio rerio(zebrafish)
Product	netrin-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY928518.1. ##Evidence-Data-START## CDS exon combination :: AY928518.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA3505370 [ECO:0000350] ##Evidence-Data-END##

ATGCTCACTG TGTTCCAAGC CTCCAGCCAC AGCCCCAATC CATTCAGCGG GCCTCAAGTG 
ACTCCGACAG ACCCCTGTTA TGAGGAGACG GGTGCTGCCC GCCGGTGCAT CCCAGAGTTC 
ATAAACGCAG CATTTGGGCA GGAGGTCAGT GTGTCGAGTG TTTGTGGGCG ACCAGTGTCT 
AGATCATGTA GTTTAGTGGA GCGTGATGAC CGTCCCGCCG TTCGCACATG CCAAATCTGC 
GATGCATCTG ATCCCCGCAA TGCCCATCCC GCCAGCTACC TCACCGACCT CAACTCTGCC 
CATAACCTCA CCTGCTGGCA GTCGGAGAAC CTGCAGAGCT CGCCGCTCAA CGTAACGCTC 
ACATTATCCC TCGGGAAGAA GTTCGAGATC ACTTATGTCA GCCTTCAGTT TTGCTCGCCA 
CGTCCTGAAT CGTTGGCCAT ATATAAAAGC ATGGACTACG GGCGCACTTG GACTCCTTAC 
CAATTCTACT CATCGCAGTG CCGTCGCATG TATAACCGAC CCAACAAAGC TGCAATCACT 
AAACAGAACG AGCAGGAGGC TTTATGCACA GACGGACACA CTGATCTCTA CCCGCTTTCT 
GGAGGTCTCA TTGCGTTTAG TACATTGGAC GGGCGCCCAT CGGGGAAGGA CTTTGACTCG 
AGTCCTGTGC TTCAGGATTG GGTGACGGTC ACTGATATTC GGGTGGTGTT CAGTCGTCCG 
CAGCACACTA GAGCGCTGCC AATGTCAGGA CGTGAAAGTG AAGACGCTCT CGGTGCGCCT 
GGAAGCTTGC CGACGTACTA TTATGCGGTT GGTGACTTCC AAGTGGGCGG GAGGTGTAAA 
TGCAATGGCC ACGCCTCCAG GTGTGTACGG GAGAAGGACG GGAAGCTGCT GTGTGAGTGC 
AAACACAACA CTGAGGGTCC GGAGTGTGAT CGATGTAAAC CGTTTCATTA TGACCGGCCC 
TGGCAGCGGG CGACAGGGAA AGAGGCCAAC GAGTGCTTAC CGTGTAACTG TAACCTTCAT 
GCGCGGCGCT GTCGCTTTAA CATGGAGTTG TACAAGCTGT CAGGCCGAAA GAGTGGAGGA 
GTGTGTATGA ACTGCAGACA CAACACTGCT GGACGCCACT GCCATTACTG CAAAGAGGGA 
TTCTATAGGG ACATGGGCAA AGCCATCACG CACCGACGGG CATGCAAAGC ATGTGACTGC 
CATCCGGTGG GAGCTGCAGG TAAGACGTGT AACCAGACGA CGGGTCAGTG TCCGTGTAAA 
GACGGAGTGA CCGGCATCAC CTGTAACCGC TGTGCTAAAG GCTATCAGCA GAGTCGATCG 
CCCGTCGCCC CGTGCATCAA AATCCCGGTT ATAAAGCCAA CGGCTGTGAT CAGCACTACA 
GAGGAGCCTG CAGATTGTGA CTCTTACTGT AAACCACTGA AGGGAAATCT CAAGATAAAC 
ATGAAGAAAT ACTGCAAAAA GGACTATGCG GTTCAGGTCA GTGTTTTAGA CATGGAGACC 
GTCGGTGACT GGGCCAAATT TTCGGTGAGC CTGATGTCTG TGTACAAGAG TCGCGGAGAC 
CCGCTGAAAA GAGGCGATCA CATGCTGTGG GTTCACATGA AGGATCTGGC CTGTAAATGT 
CCTCGGATAC ACATGGGCAA ACGCTTCCTC ATCCTGGGCA GCACTGAGGG CACGGCAAAC 
CCTGAACGCC CGGGACTGCA GGCCGACAAA AACAGCCTGG TCATCCAGTG GAGGGACATT 
TGGACGCGAC GACTGCGCAA GTTCCAGCGC AAAGAGAAGA AAGGCAAGTG CAGCAAAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032492.1
CDS	433..2235
Translation	MLTVFQASSHSPNPFSGPQVTPTDPCYEETGAARRCIPEFINAAFGQEVSVSSVCGRPVSRSCSLVERDDRPAVRTCQICDASDPRNAHPASYLTDLNSAHNLTCWQSENLQSSPLNVTLTLSLGKKFEITYVSLQFCSPRPESLAIYKSMDYGRTWTPYQFYSSQCRRMYNRPNKAAITKQNEQEALCTDGHTDLYPLSGGLIAFSTLDGRPSGKDFDSSPVLQDWVTVTDIRVVFSRPQHTRALPMSGRESEDALGAPGSLPTYYYAVGDFQVGGRCKCNGHASRCVREKDGKLLCECKHNTEGPECDRCKPFHYDRPWQRATGKEANECLPCNCNLHARRCRFNMELYKLSGRKSGGVCMNCRHNTAGRHCHYCKEGFYRDMGKAITHRRACKACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPVAPCIKIPVIKPTAVISTTEEPADCDSYCKPLKGNLKINMKKYCKKDYAVQVSVLDMETVGDWAKFSVSLMSVYKSRGDPLKRGDHMLWVHMKDLACKCPRIHMGKRFLILGSTEGTANPERPGLQADKNSLVIQWRDIWTRRLRKFQRKEKKGKCSKA

Target ORF information:

RefSeq Version	NM_001037415.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio netrin 2 (ntn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037415.1

ORF Insert Sequence:

ATGCTCACTG TGTTCCAAGC CTCCAGCCAC AGCCCCAATC CATTCAGCGG GCCTCAAGTG 
ACTCCGACAG ACCCCTGTTA TGAGGAGACG GGTGCTGCCC GCCGGTGCAT CCCAGAGTTC 
ATAAACGCAG CATTTGGGCA GGAGGTCAGT GTGTCGAGTG TTTGTGGGCG ACCAGTGTCT 
AGATCATGTA GTTTAGTGGA GCGTGATGAC CGTCCCGCCG TTCGCACATG CCAAATCTGC 
GATGCATCTG ATCCCCGCAA TGCCCATCCC GCCAGCTACC TCACCGACCT CAACTCTGCC 
CATAACCTCA CCTGCTGGCA GTCGGAGAAC CTGCAGAGCT CGCCGCTCAA CGTAACGCTC 
ACATTATCCC TCGGGAAGAA GTTCGAGATC ACTTATGTCA GCCTTCAGTT TTGCTCGCCA 
CGTCCTGAAT CGTTGGCCAT ATATAAAAGC ATGGACTACG GGCGCACTTG GACTCCTTAC 
CAATTCTACT CATCGCAGTG CCGTCGCATG TATAACCGAC CCAACAAAGC TGCAATCACT 
AAACAGAACG AGCAGGAGGC TTTATGCACA GACGGACACA CTGATCTCTA CCCGCTTTCT 
GGAGGTCTCA TTGCGTTTAG TACATTGGAC GGGCGCCCAT CGGGGAAGGA CTTTGACTCG 
AGTCCTGTGC TTCAGGATTG GGTGACGGTC ACTGATATTC GGGTGGTGTT CAGTCGTCCG 
CAGCACACTA GAGCGCTGCC AATGTCAGGA CGTGAAAGTG AAGACGCTCT CGGTGCGCCT 
GGAAGCTTGC CGACGTACTA TTATGCGGTT GGTGACTTCC AAGTGGGCGG GAGGTGTAAA 
TGCAATGGCC ACGCCTCCAG GTGTGTACGG GAGAAGGACG GGAAGCTGCT GTGTGAGTGC 
AAACACAACA CTGAGGGTCC GGAGTGTGAT CGATGTAAAC CGTTTCATTA TGACCGGCCC 
TGGCAGCGGG CGACAGGGAA AGAGGCCAAC GAGTGCTTAC CGTGTAACTG TAACCTTCAT 
GCGCGGCGCT GTCGCTTTAA CATGGAGTTG TACAAGCTGT CAGGCCGAAA GAGTGGAGGA 
GTGTGTATGA ACTGCAGACA CAACACTGCT GGACGCCACT GCCATTACTG CAAAGAGGGA 
TTCTATAGGG ACATGGGCAA AGCCATCACG CACCGACGGG CATGCAAAGC ATGTGACTGC 
CATCCGGTGG GAGCTGCAGG TAAGACGTGT AACCAGACGA CGGGTCAGTG TCCGTGTAAA 
GACGGAGTGA CCGGCATCAC CTGTAACCGC TGTGCTAAAG GCTATCAGCA GAGTCGATCG 
CCCGTCGCCC CGTGCATCAA AATCCCGGTT ATAAAGCCAA CGGCTGTGAT CAGCACTACA 
GAGGAGCCTG CAGATTGTGA CTCTTACTGT AAACCACTGA AGGGAAATCT CAAGATAAAC 
ATGAAGAAAT ACTGCAAAAA GGACTATGCG GTTCAGGTCA GTGTTTTAGA CATGGAGACC 
GTCGGTGACT GGGCCAAATT TTCGGTGAGC CTGATGTCTG TGTACAAGAG TCGCGGAGAC 
CCGCTGAAAA GAGGCGATCA CATGCTGTGG GTTCACATGA AGGATCTGGC CTGTAAATGT 
CCTCGGATAC ACATGGGCAA ACGCTTCCTC ATCCTGGGCA GCACTGAGGG CACGGCAAAC 
CCTGAACGCC CGGGACTGCA GGCCGACAAA AACAGCCTGG TCATCCAGTG GAGGGACATT 
TGGACGCGAC GACTGCGCAA GTTCCAGCGC AAAGAGAAGA AAGGCAAGTG CAGCAAAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tris(1,3-dichloro-2-propyl) phosphate disrupts axonal growth, cholinergic system and motor behavior in early life zebrafish.
Aquatic toxicology (Amsterdam, Netherlands)1927-15(2017 Nov)
Cheng R,Jia Y,Dai L,Liu C,Wang J,Li G,Yu L

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A Floor-Plate Extracellular Protein-Protein Interaction Screen Identifies Draxin as a Secreted Netrin-1 Antagonist.
Cell reports12(4)694-708(2015 Jul)
Gao X,Metzger U,Panza P,Mahalwar P,Alsheimer S,Geiger H,Maischein HM,Levesque MP,Templin M,S?llner C

Netrin signaling breaks the equivalence between two identified zebrafish motoneurons revealing a new role of intermediate targets.
PloS one6(10)e25841(2011)
Hale LA,Fowler DK,Eisen JS

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ntn2 cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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