mRpL14 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mRpL14
Entrez Gene ID	31222
Full Name	mitochondrial ribosomal protein L14
Synonyms	BcDNA:RH18819,CG14048,Dmel\CG14048,EG:BACH48C10.6,L14,mRPL14
General protein information	Preferred Names mitochondrial ribosomal protein L14 Names CG14048 gene product from transcript CG14048-RB CG14048-PA CG14048-PB mRpL14-PA mRpL14-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3A1-3A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272257.2	NP_001259186.1	mitochondrial ribosomal protein L14, isoform B
NM_080309.4	NP_525048.1	mitochondrial ribosomal protein L14, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MRPL14	XP_005627456.1
Mus musculus (house mouse)	Mrpl14	NP_081008.1
Rattus norvegicus (Norway rat)	Mrpl14	NP_001100360.1
Homo sapiens (human)	MRPL14	NP_115487.2
	LOC698527	XP_001087026.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011148	XP_564374.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011146	XP_309502.3
Xenopus tropicalis (tropical clawed frog)	mrpl14	NP_001165105.1
Pan troglodytes (chimpanzee)	MRPL14	XP_001140769.3
Bos taurus (cattle)	MRPL14	NP_001069805.1
Danio rerio (zebrafish)	mrpl14	NP_957441.1
Drosophila melanogaster (fruit fly)	mRpL14	NP_525048.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased mutant gene dosage can thus compensate the mutant phenotype, but only when located in its normal chromosomal context
Title: Phenotypic suppression of the Drosophila mitochondrial disease-like mutant tko(25t) by duplication of the mutant gene in its natural chromosomal context.

The following mRpL14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mRpL14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00598	NM_001272257.2 Latest version!	Drosophila melanogaster mitochondrial ribosomal protein L14 (mRpL14), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa00598	NM_080309.4 Latest version!	Drosophila melanogaster mitochondrial ribosomal protein L14 (mRpL14), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mRpL14 ( NM_001272257.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259186.1 Drosophila melanogaster mitochondrial ribosomal protein L14 (mRpL14), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00598
Clone ID Related Accession (Same CDS sequence)	NM_001272257.2 , NM_080309.4
Accession Version	NM_001272257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial ribosomal protein L14, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272257.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTGC GCATCCTGAA AACAGTTAGA CAAGTGGCCC AACCACTGGC GGTAACCCTG 
GGCGGCCAGC AAACACAGCA ATTGATTCAC ACAACGCCGG CGTGCTGCGA AATCCGGAAA 
TTGGCCCGGT TACGGGTGGT GGACAACAGC GATTTGGGAA AGAAAGCGAT GGCCGAGGGG 
CGACCACCGA GGTGCATCCA TGTCTACAAC AAACGCGGAG TCGGCTTCAT TGGCGATAAG 
GTGCTGGTGG CCATCAAGGG ACAGATGAAG AAGGGCATTC TCGTGGGACT CAAGCAGAAC 
CAGAAGCCCA AACAGCCGAA ATTCGATAGC AACAACCTGG TGCTCATCGA CGACAATGGC 
AGTCCGCTGG GCACTCGCAT CCATGTGCCC ATTCCCACGA TCCTGCGAAC CATCCTCAAG 
GAGAAGACGC TGGCCAAAGG AGCCGATTAC ACCAAGGTTC TGGCCATCGC CAGCAGATAT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259186.1
CDS	59..544
Translation	MALRILKTVRQVAQPLAVTLGGQQTQQLIHTTPACCEIRKLARLRVVDNSDLGKKAMAEGRPPRCIHVYNKRGVGFIGDKVLVAIKGQMKKGILVGLKQNQKPKQPKFDSNNLVLIDDNGSPLGTRIHVPIPTILRTILKEKTLAKGADYTKVLAIASRYV

Target ORF information:

RefSeq Version	NM_001272257.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial ribosomal protein L14 (mRpL14), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272257.2

ORF Insert Sequence:

ATGGCGCTGC GCATCCTGAA AACAGTTAGA CAAGTGGCCC AACCACTGGC GGTAACCCTG 
GGCGGCCAGC AAACACAGCA ATTGATTCAC ACAACGCCGG CGTGCTGCGA AATCCGGAAA 
TTGGCCCGGT TACGGGTGGT GGACAACAGC GATTTGGGAA AGAAAGCGAT GGCCGAGGGG 
CGACCACCGA GGTGCATCCA TGTCTACAAC AAACGCGGAG TCGGCTTCAT TGGCGATAAG 
GTGCTGGTGG CCATCAAGGG ACAGATGAAG AAGGGCATTC TCGTGGGACT CAAGCAGAAC 
CAGAAGCCCA AACAGCCGAA ATTCGATAGC AACAACCTGG TGCTCATCGA CGACAATGGC 
AGTCCGCTGG GCACTCGCAT CCATGTGCCC ATTCCCACGA TCCTGCGAAC CATCCTCAAG 
GAGAAGACGC TGGCCAAAGG AGCCGATTAC ACCAAGGTTC TGGCCATCGC CAGCAGATAT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mRpL14 ( NM_080309.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525048.1 Drosophila melanogaster mitochondrial ribosomal protein L14 (mRpL14), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00598
Clone ID Related Accession (Same CDS sequence)	NM_001272257.2 , NM_080309.4
Accession Version	NM_080309.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial ribosomal protein L14, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_080309.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTGC GCATCCTGAA AACAGTTAGA CAAGTGGCCC AACCACTGGC GGTAACCCTG 
GGCGGCCAGC AAACACAGCA ATTGATTCAC ACAACGCCGG CGTGCTGCGA AATCCGGAAA 
TTGGCCCGGT TACGGGTGGT GGACAACAGC GATTTGGGAA AGAAAGCGAT GGCCGAGGGG 
CGACCACCGA GGTGCATCCA TGTCTACAAC AAACGCGGAG TCGGCTTCAT TGGCGATAAG 
GTGCTGGTGG CCATCAAGGG ACAGATGAAG AAGGGCATTC TCGTGGGACT CAAGCAGAAC 
CAGAAGCCCA AACAGCCGAA ATTCGATAGC AACAACCTGG TGCTCATCGA CGACAATGGC 
AGTCCGCTGG GCACTCGCAT CCATGTGCCC ATTCCCACGA TCCTGCGAAC CATCCTCAAG 
GAGAAGACGC TGGCCAAAGG AGCCGATTAC ACCAAGGTTC TGGCCATCGC CAGCAGATAT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525048.1
CDS	115..600
Translation	MALRILKTVRQVAQPLAVTLGGQQTQQLIHTTPACCEIRKLARLRVVDNSDLGKKAMAEGRPPRCIHVYNKRGVGFIGDKVLVAIKGQMKKGILVGLKQNQKPKQPKFDSNNLVLIDDNGSPLGTRIHVPIPTILRTILKEKTLAKGADYTKVLAIASRYV

Target ORF information:

RefSeq Version	NM_080309.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial ribosomal protein L14 (mRpL14), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080309.4

ORF Insert Sequence:

ATGGCGCTGC GCATCCTGAA AACAGTTAGA CAAGTGGCCC AACCACTGGC GGTAACCCTG 
GGCGGCCAGC AAACACAGCA ATTGATTCAC ACAACGCCGG CGTGCTGCGA AATCCGGAAA 
TTGGCCCGGT TACGGGTGGT GGACAACAGC GATTTGGGAA AGAAAGCGAT GGCCGAGGGG 
CGACCACCGA GGTGCATCCA TGTCTACAAC AAACGCGGAG TCGGCTTCAT TGGCGATAAG 
GTGCTGGTGG CCATCAAGGG ACAGATGAAG AAGGGCATTC TCGTGGGACT CAAGCAGAAC 
CAGAAGCCCA AACAGCCGAA ATTCGATAGC AACAACCTGG TGCTCATCGA CGACAATGGC 
AGTCCGCTGG GCACTCGCAT CCATGTGCCC ATTCCCACGA TCCTGCGAAC CATCCTCAAG 
GAGAAGACGC TGGCCAAAGG AGCCGATTAC ACCAAGGTTC TGGCCATCGC CAGCAGATAT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mRpL14 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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