bif cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bif
Entrez Gene ID	32119
Full Name	bifocal
Synonyms	Bif,CG1822,Dmel\CG1822
General protein information	Preferred Names bifocal Names CG1822 gene product from transcript CG1822-RE CG1822-PB CG1822-PC CG1822-PD CG1822-PE CG1822-PF bif-PB bif-PC bif-PD bif-PE bif-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10D4-10D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272527.2	NP_001259456.1	bifocal, isoform E
NM_167295.3	NP_727547.1	bifocal, isoform B
NM_078573.4	NP_511128.2	bifocal, isoform C
NM_001272528.2	NP_001259457.1	bifocal, isoform F
NM_001272526.2	NP_001259455.1	bifocal, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bif regulates PP1 activity, and both proteins influence cytoskeleton dynamics in the growth cone of R cells to allow proper axon targeting.
Title: Bifocal and PP1 interaction regulates targeting of the R-cell growth cone in Drosophila.

Data suggest that Bifocal functions downstream of Misshapen to reorganize actin cytoskeleton in decelerating R cell growth cone motility at the target region.
Title: Bifocal is a downstream target of the Ste20-like serine/threonine kinase misshapen in regulating photoreceptor growth cone targeting in Drosophila.

The following bif gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bif cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01307	NM_078573.4 Latest version!	Drosophila melanogaster bifocal (bif), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa01308	NM_167295.3 Latest version!	Drosophila melanogaster bifocal (bif), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa02790	NM_001272527.2 Latest version!	Drosophila melanogaster bifocal (bif), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa02791	NM_001272528.2 Latest version!	Drosophila melanogaster bifocal (bif), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa02792	NM_001272526.2 Latest version!	Drosophila melanogaster bifocal (bif), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bif ( NM_078573.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511128.2 Drosophila melanogaster bifocal (bif), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01307
Clone ID Related Accession (Same CDS sequence)	NM_078573.4
Accession Version	NM_078573.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifocal, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078573.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGAAGCCAAC 
GCAGCTCCAG GTGCAATTGC AGATTTCACA GAACCGGCGA CAATCAGTAG CACTAGTCAA 
AAGAGAAACA TGATCGGTTC AGAGGAAAAG TCTGAGAAAT CTTCTATTTC CAATACCAAT 
TCCGATTCCA CTGGAGGTCA TCACTCTGTT GTTGCCGTCT CCCTTTCGCC CGATGCGGCA 
GCAACAACAA ATGTAACAGT AACACCAATA CCAAAGCAGC GATCGAGTTT ACTCAACACA 
AGAAGTCAGG AGAGGGAGAT GGTGCGATAT ATTCTAAGCG AGAGTGGAGA ACGGGATGGA 
GAGCTTGAGA GCGGCGAACA GCCGGCTGGT GTGGTGAGTA ACAGCCGGTG CGGTGAAGTT 
GAAACTGGCA CAATTGGATC GCCGTCGTCG TCAGCAAATC AAAATCCAAA CCCAAATCAT 
TTAAAAACGA AATGCAAACC GGGACAGAGC GTTGCTGAGG GCAAGCCTTC AGCTAAAGAG 
ACCATCGTCG ATAACAGCAA AAGCTGCAGC AAAACCAAGA GTATTTCCGA TAAATTGCAG 
AGCAACAAGT TTATAATTCA ACAGCAGCAG CAACAACAAC AACAACAGCA GCAACAACAG 
CAACTGTCGC CCACAAAGGT AACGGTAAAA CCGACAATGG TCGCCATGCA AGAGATGAAG 
AAGACAACCA AACAAAATGG CCAGCACCGA CATCTAGCGG GCAAAATTGG CAGCGTAGCA 
GGAGGCGATG TGGATCCACA ACATCCACCG ACGAATCCCA TACCAGATTC ATTGGACACC 
GGTGAGGATC TGAGCTACGG ACCCGGAATT GTGTCCAAGC TGCGATGCCG CTACTTGAGC 
CTGGCCCTTC GCGAGTCCCG CCAGCAGAGC AGCAAACAAC GCCTCCAACG CTCCACCAGT 
CTGAACACCC TGCTCGACCG CGACGATGAC GAGGTGGAAG TGGAGGAGCC CGAAATGACC 
AACAGCCAGG TGCGTGCCAA ATCAACACCG CCACCGATAT TGGGGGCAAA ACCGACACCG 
AAACCCAGCA GCCAGCATCA GCGTCCCGTC AGTCTGGGCG CCAATGGAGC TGGATCAGCT 
GTTAACCCGC CCTCGAACTC GGATCAGGCC CAAACGGGCG CCGTTGCCAA CGGAGGCGCA 
GGATCTGGCC AACGTTCGCG TCACTTTAAG CGCGGCAACG AGGTGATGAA GCGGGCACGT 
TCCGTGGAGG CTCTGCTCTG CGAGAAATCG CCATGGAACA GCCAGAGAAT CAGCACTGCC 
GGCGCAGCTG CAGCAGCAGC ACCTTCACCA CCTGCTCCCA AGGCCACAGC CGCTGCTCCC 
TCACCTGTTA CATCGCCCAC CTGCGTTACC ATCGAGGACA AGATCCACAA TGCCCGCGAA 
CGACTGCACA GCGGGACGGA TACGGCGCCA CCCAAGCGTC TGACCTCCAT CATCGATGAC 
ACCGAGCGGC CGCCACCGGA TCTGGTCAAG CAGACGCTCA AGATGTTTGA GGCGAGTGCT 
AATCGACGAC CGCGCGCAGC CCATCGTTCC AATGGTGTTG GCGGAGTGGC CAGCAAGGTG 
GCCAACTACA AATCGATCAT TAAGGATCAG AAGCAACCAT CATCAGCAGC GGCGACGCCA 
CCGCCGACCG GCATGGGTTT TGCCTCCTCA ACGCCGCTAA GGCATGTCCA TCCGGATATT 
ATACCCCGTC AGGTGGATTC GCCCGTATCG GCGTTGAGTG TAATGATGCG TCGCATGGAG 
CTGCAGGAGC CCGAGACGCC GGAAAGGGAG ACACGGGACG CGGAGGCCAC ACCGCAGAGC 
GAGGCGCTAA GTGAGACTGA GCGAAATGAT CGCGATGAAG GCGATGGCGA AGTCGATGAC 
GACAACCACA ACAACAACGA CGACGATCAC GACGACGGCG ACGACGGCAG CGACAACAAT 
GAGCAACGCG ATAAAATGGG CGCGGCGGCG GCAGGCGATA AGCCTAAGCC CCCAGCGGAA 
TCGGCAGCGG GAGGCGCCCA ACGATCATTC GCTGCCTCGA CGGAGAACGC GCATGTAGCC 
AGCGGTAGTA GCAGCAGTAC CAGTGCCAGT GTACGGAAGC TCAGCAACAA CAACGACTCC 
AGCAGCGGAC CAGCGGTGAC CAAACAGATC GGTGTCATCC GTCCGCTATT CAATAGCCAG 
GGAGCCGGGA GCACGCCGCT AACGAGTCGC GAGATCGAAA AGAATCGCAT CAATGAGATG 
AAGAAGTCGA CGGCCACAGA TGCCGGTGGT TCGGTATCCG GATCCGGAAC GGTTGCTGGT 
GCTGGATCAG GAACCTCGCC CACCACTAGT CTCGACAGCG TGATAAACAC CAAGGAAGCG 
GCCAGCAGCG AAACGGATGC CAGTGCCTCG CCACTGTGGA CATTGCGCAA GCTGAGGAAT 
CAGAGCCAGA CGGCCGGCGG AGGATCGGGT CCCAGTTCGA GCCTTCATTC CACAGAGAAC 
ACCTCGATGG TGTTCAACTT TTCCAAGAGC ACCAAGGAAG TGCCCGACTA CATCGAAAGC 
GACGTTGTGA TTTACAGGCG CAAGCGGGAG CTGCCAAAGC CAAATGAACC TGGCTTCGTG 
CTCCTGGGCG ATCTCTCCGT GGAGACGTCG ACGGACACGG ACTACGACGA CTACTCCATG 
TGCCCGCCAT CGCCGTGCGA TGTGGAGTTT GAGAATGCCA ACATTGTGAT CGACGGCAAG 
TCCAGCATAC GCCAGAAACC CAAAGAGTCT TCGTTCCGCG TGCAGTTCAA CGACACGCTG 
ACGTCGACGT TTGAATACCC CTCCGAGGCA TCGATGACCA TCGAGGATCC GCCGTACGCC 
GATCCCTTTG GCCATGTGAG CAAACACCAT CAGATGCTGC TCGCCGAGCA GATGCATCTG 
TTGCAGCACC AGGAGCAGCT GGAGCTGGAG CAGATGCACC AGCTGGGGCT GGCGCCGGAG 
CAGCACCATC ATGTGACCGT CGACGAGATC ATCGAGCTGC CCACATCGAC GGCGGGACAT 
GGACATGGCA TCGGACTTGG ACACAGGTCG GGGGCGGCGG GGGGCGGTGG CACGATGCTG 
GGGAATTTAC CGTTGGATAT TATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511128.2
CDS	330..3656
Translation	MESQKRPSLDLHTDVPAGLAAGGSGLGAAAEMSPTSGFLPDMPQWKKDLIQRRKTNVARTQAASITSPTDGSCGALAEANAAPGAIADFTEPATISSTSQKRNMIGSEEKSEKSSISNTNSDSTGGHHSVVAVSLSPDAAATTNVTVTPIPKQRSSLLNTRSQEREMVRYILSESGERDGELESGEQPAGVVSNSRCGEVETGTIGSPSSSANQNPNPNHLKTKCKPGQSVAEGKPSAKETIVDNSKSCSKTKSISDKLQSNKFIIQQQQQQQQQQQQQQQLSPTKVTVKPTMVAMQEMKKTTKQNGQHRHLAGKIGSVAGGDVDPQHPPTNPIPDSLDTGEDLSYGPGIVSKLRCRYLSLALRESRQQSSKQRLQRSTSLNTLLDRDDDEVEVEEPEMTNSQVRAKSTPPPILGAKPTPKPSSQHQRPVSLGANGAGSAVNPPSNSDQAQTGAVANGGAGSGQRSRHFKRGNEVMKRARSVEALLCEKSPWNSQRISTAGAAAAAAPSPPAPKATAAAPSPVTSPTCVTIEDKIHNARERLHSGTDTAPPKRLTSIIDDTERPPPDLVKQTLKMFEASANRRPRAAHRSNGVGGVASKVANYKSIIKDQKQPSSAAATPPPTGMGFASSTPLRHVHPDIIPRQVDSPVSALSVMMRRMELQEPETPERETRDAEATPQSEALSETERNDRDEGDGEVDDDNHNNNDDDHDDGDDGSDNNEQRDKMGAAAAGDKPKPPAESAAGGAQRSFAASTENAHVASGSSSSTSASVRKLSNNNDSSSGPAVTKQIGVIRPLFNSQGAGSTPLTSREIEKNRINEMKKSTATDAGGSVSGSGTVAGAGSGTSPTTSLDSVINTKEAASSETDASASPLWTLRKLRNQSQTAGGGSGPSSSLHSTENTSMVFNFSKSTKEVPDYIESDVVIYRRKRELPKPNEPGFVLLGDLSVETSTDTDYDDYSMCPPSPCDVEFENANIVIDGKSSIRQKPKESSFRVQFNDTLTSTFEYPSEASMTIEDPPYADPFGHVSKHHQMLLAEQMHLLQHQEQLELEQMHQLGLAPEQHHHVTVDEIIELPTSTAGHGHGIGLGHRSGAAGGGGTMLGNLPLDII

Target ORF information:

RefSeq Version	NM_078573.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifocal (bif), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078573.4

ORF Insert Sequence:

ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGAAGCCAAC 
GCAGCTCCAG GTGCAATTGC AGATTTCACA GAACCGGCGA CAATCAGTAG CACTAGTCAA 
AAGAGAAACA TGATCGGTTC AGAGGAAAAG TCTGAGAAAT CTTCTATTTC CAATACCAAT 
TCCGATTCCA CTGGAGGTCA TCACTCTGTT GTTGCCGTCT CCCTTTCGCC CGATGCGGCA 
GCAACAACAA ATGTAACAGT AACACCAATA CCAAAGCAGC GATCGAGTTT ACTCAACACA 
AGAAGTCAGG AGAGGGAGAT GGTGCGATAT ATTCTAAGCG AGAGTGGAGA ACGGGATGGA 
GAGCTTGAGA GCGGCGAACA GCCGGCTGGT GTGGTGAGTA ACAGCCGGTG CGGTGAAGTT 
GAAACTGGCA CAATTGGATC GCCGTCGTCG TCAGCAAATC AAAATCCAAA CCCAAATCAT 
TTAAAAACGA AATGCAAACC GGGACAGAGC GTTGCTGAGG GCAAGCCTTC AGCTAAAGAG 
ACCATCGTCG ATAACAGCAA AAGCTGCAGC AAAACCAAGA GTATTTCCGA TAAATTGCAG 
AGCAACAAGT TTATAATTCA ACAGCAGCAG CAACAACAAC AACAACAGCA GCAACAACAG 
CAACTGTCGC CCACAAAGGT AACGGTAAAA CCGACAATGG TCGCCATGCA AGAGATGAAG 
AAGACAACCA AACAAAATGG CCAGCACCGA CATCTAGCGG GCAAAATTGG CAGCGTAGCA 
GGAGGCGATG TGGATCCACA ACATCCACCG ACGAATCCCA TACCAGATTC ATTGGACACC 
GGTGAGGATC TGAGCTACGG ACCCGGAATT GTGTCCAAGC TGCGATGCCG CTACTTGAGC 
CTGGCCCTTC GCGAGTCCCG CCAGCAGAGC AGCAAACAAC GCCTCCAACG CTCCACCAGT 
CTGAACACCC TGCTCGACCG CGACGATGAC GAGGTGGAAG TGGAGGAGCC CGAAATGACC 
AACAGCCAGG TGCGTGCCAA ATCAACACCG CCACCGATAT TGGGGGCAAA ACCGACACCG 
AAACCCAGCA GCCAGCATCA GCGTCCCGTC AGTCTGGGCG CCAATGGAGC TGGATCAGCT 
GTTAACCCGC CCTCGAACTC GGATCAGGCC CAAACGGGCG CCGTTGCCAA CGGAGGCGCA 
GGATCTGGCC AACGTTCGCG TCACTTTAAG CGCGGCAACG AGGTGATGAA GCGGGCACGT 
TCCGTGGAGG CTCTGCTCTG CGAGAAATCG CCATGGAACA GCCAGAGAAT CAGCACTGCC 
GGCGCAGCTG CAGCAGCAGC ACCTTCACCA CCTGCTCCCA AGGCCACAGC CGCTGCTCCC 
TCACCTGTTA CATCGCCCAC CTGCGTTACC ATCGAGGACA AGATCCACAA TGCCCGCGAA 
CGACTGCACA GCGGGACGGA TACGGCGCCA CCCAAGCGTC TGACCTCCAT CATCGATGAC 
ACCGAGCGGC CGCCACCGGA TCTGGTCAAG CAGACGCTCA AGATGTTTGA GGCGAGTGCT 
AATCGACGAC CGCGCGCAGC CCATCGTTCC AATGGTGTTG GCGGAGTGGC CAGCAAGGTG 
GCCAACTACA AATCGATCAT TAAGGATCAG AAGCAACCAT CATCAGCAGC GGCGACGCCA 
CCGCCGACCG GCATGGGTTT TGCCTCCTCA ACGCCGCTAA GGCATGTCCA TCCGGATATT 
ATACCCCGTC AGGTGGATTC GCCCGTATCG GCGTTGAGTG TAATGATGCG TCGCATGGAG 
CTGCAGGAGC CCGAGACGCC GGAAAGGGAG ACACGGGACG CGGAGGCCAC ACCGCAGAGC 
GAGGCGCTAA GTGAGACTGA GCGAAATGAT CGCGATGAAG GCGATGGCGA AGTCGATGAC 
GACAACCACA ACAACAACGA CGACGATCAC GACGACGGCG ACGACGGCAG CGACAACAAT 
GAGCAACGCG ATAAAATGGG CGCGGCGGCG GCAGGCGATA AGCCTAAGCC CCCAGCGGAA 
TCGGCAGCGG GAGGCGCCCA ACGATCATTC GCTGCCTCGA CGGAGAACGC GCATGTAGCC 
AGCGGTAGTA GCAGCAGTAC CAGTGCCAGT GTACGGAAGC TCAGCAACAA CAACGACTCC 
AGCAGCGGAC CAGCGGTGAC CAAACAGATC GGTGTCATCC GTCCGCTATT CAATAGCCAG 
GGAGCCGGGA GCACGCCGCT AACGAGTCGC GAGATCGAAA AGAATCGCAT CAATGAGATG 
AAGAAGTCGA CGGCCACAGA TGCCGGTGGT TCGGTATCCG GATCCGGAAC GGTTGCTGGT 
GCTGGATCAG GAACCTCGCC CACCACTAGT CTCGACAGCG TGATAAACAC CAAGGAAGCG 
GCCAGCAGCG AAACGGATGC CAGTGCCTCG CCACTGTGGA CATTGCGCAA GCTGAGGAAT 
CAGAGCCAGA CGGCCGGCGG AGGATCGGGT CCCAGTTCGA GCCTTCATTC CACAGAGAAC 
ACCTCGATGG TGTTCAACTT TTCCAAGAGC ACCAAGGAAG TGCCCGACTA CATCGAAAGC 
GACGTTGTGA TTTACAGGCG CAAGCGGGAG CTGCCAAAGC CAAATGAACC TGGCTTCGTG 
CTCCTGGGCG ATCTCTCCGT GGAGACGTCG ACGGACACGG ACTACGACGA CTACTCCATG 
TGCCCGCCAT CGCCGTGCGA TGTGGAGTTT GAGAATGCCA ACATTGTGAT CGACGGCAAG 
TCCAGCATAC GCCAGAAACC CAAAGAGTCT TCGTTCCGCG TGCAGTTCAA CGACACGCTG 
ACGTCGACGT TTGAATACCC CTCCGAGGCA TCGATGACCA TCGAGGATCC GCCGTACGCC 
GATCCCTTTG GCCATGTGAG CAAACACCAT CAGATGCTGC TCGCCGAGCA GATGCATCTG 
TTGCAGCACC AGGAGCAGCT GGAGCTGGAG CAGATGCACC AGCTGGGGCT GGCGCCGGAG 
CAGCACCATC ATGTGACCGT CGACGAGATC ATCGAGCTGC CCACATCGAC GGCGGGACAT 
GGACATGGCA TCGGACTTGG ACACAGGTCG GGGGCGGCGG GGGGCGGTGG CACGATGCTG 
GGGAATTTAC CGTTGGATAT TATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bif ( NM_167295.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727547.1 Drosophila melanogaster bifocal (bif), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01308
Clone ID Related Accession (Same CDS sequence)	NM_167295.3
Accession Version	NM_167295.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifocal, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167295.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGAAGCCAAC 
GCAGCTCCAG GTGCAATTGC AGATTTCACA GAACCGGCGA CAATCAGTAG CACTAGTCAA 
AAGAGAAACA TGATCGGTTC AGAGGAAAAG TCTGAGAAAT CTTCTATTTC CAATACCAAT 
TCCGATTCCA CTGGAGGTCA TCACTCTGTT GTTGCCGTCT CCCTTTCGCC CGATGCGGCA 
GCAACAACAA ATGTAACAGT AACACCAATA CCAAAGCAGC GATCGAGTTT ACTCAACACA 
AGAAGTCAGG AGAGGGAGAT GGTGCGATAT ATTCTAAGCG AGAGTGGAGA ACGGGATGGA 
GAGCTTGAGA GCGGCGAACA GCCGGCTGGT GTGGTGAGTA ACAGCCGGTG CGGTGAAGTT 
GAAACTGGCA CAATTGGATC GCCGTCGTCG TCAGCAAATC AAAATCCAAA CCCAAATCAT 
TTAAAAACGA AATGCAAACC GGGACAGAGC GTTGCTGAGG GCAAGCCTTC AGCTAAAGAG 
ACCATCGTCG ATAACAGCAA AAGCTGCAGC AAAACCAAGA GTATTTCCGA TAAATTGCAG 
AGCAACAAGT TTATAATTCA ACAGCAGCAG CAACAACAAC AACAACAGCA GCAACAACAG 
CAACTGTCGC CCACAAAGGT AACGGTAAAA CCGACAATGG TCGCCATGCA AGAGATGAAG 
AAGACAACCA AACAAAATGG CCAGCACCGA CATCTAGCGG GCAAAATTGG CAGCGTAGCA 
GGAGGCGATG TGGATCCACA ACATCCACCG ACGAATCCCA TACCAGATTC ATTGGACACC 
GGTGAGGATC TGAGCTACGG ACCCGGAATT GTGTCCAAGC TGCGATGCCG CTACTTGAGC 
CTGGCCCTTC GCGAGTCCCG CCAGCAGAGC AGCAAACAAC GCCTCCAACG CTCCACCAGT 
CTGAACACCC TGCTCGACCG CGACGATGAC GAGGTGGAAG TGGAGGAGCC CGAAATGACC 
AACAGCCAGG TGCGTGCCAA ATCAACACCG CCACCGATAT TGGGGGCAAA ACCGACACCG 
AAACCCAGCA GCCAGCATCA GCGTCCCGTC AGTCTGGGCG CCAATGGAGC TGGATCAGCT 
GTTAACCCGC CCTCGAACTC GGATCAGGCC CAAACGGGCG CCGTTGCCAA CGGAGGCGCA 
GGATCTGGCC AACGTTCGCG TCACTTTAAG CGCGGCAACG AGGTGATGAA GCGGGCACGT 
TCCGTGGAGG CTCTGCTCTG CGAGAAATCG CCATGGAACA GCCAGAGAAT CAGCACTGCC 
GGCGCAGCTG CAGCAGCAGC ACCTTCACCA CCTGCTCCCA AGGCCACAGC CGCTGCTCCC 
TCACCTGTTA CATCGCCCAC CTGCGTTACC ATCGAGGACA AGATCCACAA TGCCCGCGAA 
CGACTGCACA GCGGGACGGA TACGGCGCCA CCCAAGCGTC TGACCTCCAT CATCGATGAC 
ACCGAGCGGC CGCCACCGGA TCTGGTCAAG CAGACGCTCA AGATGTTTGA GGCGAGTGCT 
AATCGACGAC CGCGCGCAGC CCATCGTTCC AATGGTGTTG GCGGAGTGGC CAGCAAGGTG 
GCCAACTACA AATCGATCAT TAAGGATCAG AAGCAACCAT CATCAGCAGC GGCGACGCCA 
CCGCCGACCG GCATGGGTTT TGCCTCCTCA ACGCCGCTAA GGCATGTCCA TCCGGATATT 
ATACCCCGTC AGGTGGATTC GCCCGTATCG GCGTTGAGTG TAATGATGCG TCGCATGGAG 
CTGCAGGAGC CCGAGACGCC GGAAAGGGAG ACACGGGACG CGGAGGCCAC ACCGCAGAGC 
GAGGCGCTAA GTGAGACTGA GCGAAATGAT CGCGATGAAG GCGATGGCGA AGTCGATGAC 
GACAACCACA ACAACAACGA CGACGATCAC GACGACGGCG ACGACGGCAG CGACAACAAT 
GAGCAACGCG ATAAAATGGG CGCGGCGGCG GCAGGCGATA AGCCTAAGCC CCCAGCGGAA 
TCGGCAGCGG GAGGCGCCCA ACGATCATTC GCTGCCTCGA CGGAGAACGC GCATGTAGCC 
AGCGGTAGTA GCAGCAGTAC CAGTGCCAGT GTACGGAAGC TCAGCAACAA CAACGACTCC 
AGCAGCGGAC CAGCGGTGAC CAAACAGATC GGTGTCATCC GTCCGCTATT CAATAGCCAG 
GGAGCCGGGA GCACGCCGCT AACGAGTCGC GAGATCGAAA AGAATCGCAT CAATGAGATG 
AAGAAGTCGA CGGCCACAGA TGCCGGTGGT TCGGTATCCG GATCCGGAAC GGTTGCTGGT 
GCTGGATCAG GAACCTCGCC CACCACTAGT CTCGACAGCG TGATAAACAC CAAGGAAGCG 
GCCAGCAGCG AAACGGATGC CAGTGCCTCG CCACTGTGGA CATTGCGCAA GCTGAGGAAT 
CAGAGCCAGA CGGCCGGCGG AGGATCGGGT CCCAGTTCGA GCCTTCATTC CACAGAGAAC 
ACCTCGATGG TGTTCAACTT TTCCAAGAGC ACCAAGGAAG TGCCCGACTA CATCGAAAGC 
GACGTTGTGA TTTACAGGCG CAAGCGGGAG CTGCCAAAGC CAAATGAACC TGGCTTCGTG 
CTCCTGGGCG ATCTCTCCGT GGAGACGTCG ACGGACACGG ACTACGACGA CTACTCCATG 
TGCCCGCCAT CGCCGTGCGA TGTGGAGTTT GAGAATGCCA ACATTGTGAT CGACGGCAAG 
TCCAGCATAC GCCAGAAACC CAAAGAGTCT TCGTTCCGCG TGCAGTTCAA CGACACGCTG 
ACGTCGACGT TTGAATACCC CTCCGAGGCA TCGATGACCA TCGAGGATCC GCCGTACGCC 
GATCCCTTTG GCCATGTGAG CAAACACCAT CAGATGCTGC TCGCCGAGCA GATGCATCTG 
TTGCAGCACC AGGAGCAGCT GGAGCTGGAG CAGATGCACC AGCTGGGGCT GGCGCCGGAG 
CAGCACCATC ATGTGACCGT CGACGAGATC ATCGAGCTGC CCACATCGAC GGCGGGACAT 
GGACATGGCA TCGGACTTGG ACACAGGTCG GGGGCGGCGG GGGGCGGTGG CACGATGCTG 
GGGAATTTAC CGTTGGGCTC CACGGCTCTG GGCTTCTACA CGCCCATGAA GGGCACGGCG 
ATGGACAACC TATTCCAGCT GGGTGTCACC CGATACGCCC TGCCCGAGAG CAGCAGCAGT 
GGGAGCAGCA ATGGCAGCCA CAGTCCAGGC AGCAACGGGA GCAGTCCAGC CGGAGCTGCG 
GGCAGCCGGT TGATGTTCAA CGGGAATGGC AGCATTATGG GCATCGGCGA GGGACTGATC 
AAGGAGGGCG ACTTGGCGGC AACGGAAGCA GGACCTGGAA CTGCAGATGG AGCAGGTGCC 
TCCAAGAAGG GTTCAGCTGT GGACGAGGAT GATTACCATC TGACGGCGAC GCCGGGCGCC 
GAAGTTGTGT ACAGCGAGGG TACACAGAAA ACGGATTTGC TATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727547.1
CDS	330..4037
Translation	MESQKRPSLDLHTDVPAGLAAGGSGLGAAAEMSPTSGFLPDMPQWKKDLIQRRKTNVARTQAASITSPTDGSCGALAEANAAPGAIADFTEPATISSTSQKRNMIGSEEKSEKSSISNTNSDSTGGHHSVVAVSLSPDAAATTNVTVTPIPKQRSSLLNTRSQEREMVRYILSESGERDGELESGEQPAGVVSNSRCGEVETGTIGSPSSSANQNPNPNHLKTKCKPGQSVAEGKPSAKETIVDNSKSCSKTKSISDKLQSNKFIIQQQQQQQQQQQQQQQLSPTKVTVKPTMVAMQEMKKTTKQNGQHRHLAGKIGSVAGGDVDPQHPPTNPIPDSLDTGEDLSYGPGIVSKLRCRYLSLALRESRQQSSKQRLQRSTSLNTLLDRDDDEVEVEEPEMTNSQVRAKSTPPPILGAKPTPKPSSQHQRPVSLGANGAGSAVNPPSNSDQAQTGAVANGGAGSGQRSRHFKRGNEVMKRARSVEALLCEKSPWNSQRISTAGAAAAAAPSPPAPKATAAAPSPVTSPTCVTIEDKIHNARERLHSGTDTAPPKRLTSIIDDTERPPPDLVKQTLKMFEASANRRPRAAHRSNGVGGVASKVANYKSIIKDQKQPSSAAATPPPTGMGFASSTPLRHVHPDIIPRQVDSPVSALSVMMRRMELQEPETPERETRDAEATPQSEALSETERNDRDEGDGEVDDDNHNNNDDDHDDGDDGSDNNEQRDKMGAAAAGDKPKPPAESAAGGAQRSFAASTENAHVASGSSSSTSASVRKLSNNNDSSSGPAVTKQIGVIRPLFNSQGAGSTPLTSREIEKNRINEMKKSTATDAGGSVSGSGTVAGAGSGTSPTTSLDSVINTKEAASSETDASASPLWTLRKLRNQSQTAGGGSGPSSSLHSTENTSMVFNFSKSTKEVPDYIESDVVIYRRKRELPKPNEPGFVLLGDLSVETSTDTDYDDYSMCPPSPCDVEFENANIVIDGKSSIRQKPKESSFRVQFNDTLTSTFEYPSEASMTIEDPPYADPFGHVSKHHQMLLAEQMHLLQHQEQLELEQMHQLGLAPEQHHHVTVDEIIELPTSTAGHGHGIGLGHRSGAAGGGGTMLGNLPLGSTALGFYTPMKGTAMDNLFQLGVTRYALPESSSSGSSNGSHSPGSNGSSPAGAAGSRLMFNGNGSIMGIGEGLIKEGDLAATEAGPGTADGAGASKKGSAVDEDDYHLTATPGAEVVYSEGTQKTDLLY

Target ORF information:

RefSeq Version	NM_167295.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifocal (bif), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167295.3

ORF Insert Sequence:

ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGAAGCCAAC 
GCAGCTCCAG GTGCAATTGC AGATTTCACA GAACCGGCGA CAATCAGTAG CACTAGTCAA 
AAGAGAAACA TGATCGGTTC AGAGGAAAAG TCTGAGAAAT CTTCTATTTC CAATACCAAT 
TCCGATTCCA CTGGAGGTCA TCACTCTGTT GTTGCCGTCT CCCTTTCGCC CGATGCGGCA 
GCAACAACAA ATGTAACAGT AACACCAATA CCAAAGCAGC GATCGAGTTT ACTCAACACA 
AGAAGTCAGG AGAGGGAGAT GGTGCGATAT ATTCTAAGCG AGAGTGGAGA ACGGGATGGA 
GAGCTTGAGA GCGGCGAACA GCCGGCTGGT GTGGTGAGTA ACAGCCGGTG CGGTGAAGTT 
GAAACTGGCA CAATTGGATC GCCGTCGTCG TCAGCAAATC AAAATCCAAA CCCAAATCAT 
TTAAAAACGA AATGCAAACC GGGACAGAGC GTTGCTGAGG GCAAGCCTTC AGCTAAAGAG 
ACCATCGTCG ATAACAGCAA AAGCTGCAGC AAAACCAAGA GTATTTCCGA TAAATTGCAG 
AGCAACAAGT TTATAATTCA ACAGCAGCAG CAACAACAAC AACAACAGCA GCAACAACAG 
CAACTGTCGC CCACAAAGGT AACGGTAAAA CCGACAATGG TCGCCATGCA AGAGATGAAG 
AAGACAACCA AACAAAATGG CCAGCACCGA CATCTAGCGG GCAAAATTGG CAGCGTAGCA 
GGAGGCGATG TGGATCCACA ACATCCACCG ACGAATCCCA TACCAGATTC ATTGGACACC 
GGTGAGGATC TGAGCTACGG ACCCGGAATT GTGTCCAAGC TGCGATGCCG CTACTTGAGC 
CTGGCCCTTC GCGAGTCCCG CCAGCAGAGC AGCAAACAAC GCCTCCAACG CTCCACCAGT 
CTGAACACCC TGCTCGACCG CGACGATGAC GAGGTGGAAG TGGAGGAGCC CGAAATGACC 
AACAGCCAGG TGCGTGCCAA ATCAACACCG CCACCGATAT TGGGGGCAAA ACCGACACCG 
AAACCCAGCA GCCAGCATCA GCGTCCCGTC AGTCTGGGCG CCAATGGAGC TGGATCAGCT 
GTTAACCCGC CCTCGAACTC GGATCAGGCC CAAACGGGCG CCGTTGCCAA CGGAGGCGCA 
GGATCTGGCC AACGTTCGCG TCACTTTAAG CGCGGCAACG AGGTGATGAA GCGGGCACGT 
TCCGTGGAGG CTCTGCTCTG CGAGAAATCG CCATGGAACA GCCAGAGAAT CAGCACTGCC 
GGCGCAGCTG CAGCAGCAGC ACCTTCACCA CCTGCTCCCA AGGCCACAGC CGCTGCTCCC 
TCACCTGTTA CATCGCCCAC CTGCGTTACC ATCGAGGACA AGATCCACAA TGCCCGCGAA 
CGACTGCACA GCGGGACGGA TACGGCGCCA CCCAAGCGTC TGACCTCCAT CATCGATGAC 
ACCGAGCGGC CGCCACCGGA TCTGGTCAAG CAGACGCTCA AGATGTTTGA GGCGAGTGCT 
AATCGACGAC CGCGCGCAGC CCATCGTTCC AATGGTGTTG GCGGAGTGGC CAGCAAGGTG 
GCCAACTACA AATCGATCAT TAAGGATCAG AAGCAACCAT CATCAGCAGC GGCGACGCCA 
CCGCCGACCG GCATGGGTTT TGCCTCCTCA ACGCCGCTAA GGCATGTCCA TCCGGATATT 
ATACCCCGTC AGGTGGATTC GCCCGTATCG GCGTTGAGTG TAATGATGCG TCGCATGGAG 
CTGCAGGAGC CCGAGACGCC GGAAAGGGAG ACACGGGACG CGGAGGCCAC ACCGCAGAGC 
GAGGCGCTAA GTGAGACTGA GCGAAATGAT CGCGATGAAG GCGATGGCGA AGTCGATGAC 
GACAACCACA ACAACAACGA CGACGATCAC GACGACGGCG ACGACGGCAG CGACAACAAT 
GAGCAACGCG ATAAAATGGG CGCGGCGGCG GCAGGCGATA AGCCTAAGCC CCCAGCGGAA 
TCGGCAGCGG GAGGCGCCCA ACGATCATTC GCTGCCTCGA CGGAGAACGC GCATGTAGCC 
AGCGGTAGTA GCAGCAGTAC CAGTGCCAGT GTACGGAAGC TCAGCAACAA CAACGACTCC 
AGCAGCGGAC CAGCGGTGAC CAAACAGATC GGTGTCATCC GTCCGCTATT CAATAGCCAG 
GGAGCCGGGA GCACGCCGCT AACGAGTCGC GAGATCGAAA AGAATCGCAT CAATGAGATG 
AAGAAGTCGA CGGCCACAGA TGCCGGTGGT TCGGTATCCG GATCCGGAAC GGTTGCTGGT 
GCTGGATCAG GAACCTCGCC CACCACTAGT CTCGACAGCG TGATAAACAC CAAGGAAGCG 
GCCAGCAGCG AAACGGATGC CAGTGCCTCG CCACTGTGGA CATTGCGCAA GCTGAGGAAT 
CAGAGCCAGA CGGCCGGCGG AGGATCGGGT CCCAGTTCGA GCCTTCATTC CACAGAGAAC 
ACCTCGATGG TGTTCAACTT TTCCAAGAGC ACCAAGGAAG TGCCCGACTA CATCGAAAGC 
GACGTTGTGA TTTACAGGCG CAAGCGGGAG CTGCCAAAGC CAAATGAACC TGGCTTCGTG 
CTCCTGGGCG ATCTCTCCGT GGAGACGTCG ACGGACACGG ACTACGACGA CTACTCCATG 
TGCCCGCCAT CGCCGTGCGA TGTGGAGTTT GAGAATGCCA ACATTGTGAT CGACGGCAAG 
TCCAGCATAC GCCAGAAACC CAAAGAGTCT TCGTTCCGCG TGCAGTTCAA CGACACGCTG 
ACGTCGACGT TTGAATACCC CTCCGAGGCA TCGATGACCA TCGAGGATCC GCCGTACGCC 
GATCCCTTTG GCCATGTGAG CAAACACCAT CAGATGCTGC TCGCCGAGCA GATGCATCTG 
TTGCAGCACC AGGAGCAGCT GGAGCTGGAG CAGATGCACC AGCTGGGGCT GGCGCCGGAG 
CAGCACCATC ATGTGACCGT CGACGAGATC ATCGAGCTGC CCACATCGAC GGCGGGACAT 
GGACATGGCA TCGGACTTGG ACACAGGTCG GGGGCGGCGG GGGGCGGTGG CACGATGCTG 
GGGAATTTAC CGTTGGGCTC CACGGCTCTG GGCTTCTACA CGCCCATGAA GGGCACGGCG 
ATGGACAACC TATTCCAGCT GGGTGTCACC CGATACGCCC TGCCCGAGAG CAGCAGCAGT 
GGGAGCAGCA ATGGCAGCCA CAGTCCAGGC AGCAACGGGA GCAGTCCAGC CGGAGCTGCG 
GGCAGCCGGT TGATGTTCAA CGGGAATGGC AGCATTATGG GCATCGGCGA GGGACTGATC 
AAGGAGGGCG ACTTGGCGGC AACGGAAGCA GGACCTGGAA CTGCAGATGG AGCAGGTGCC 
TCCAAGAAGG GTTCAGCTGT GGACGAGGAT GATTACCATC TGACGGCGAC GCCGGGCGCC 
GAAGTTGTGT ACAGCGAGGG TACACAGAAA ACGGATTTGC TATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bif ( NM_001272527.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259456.1 Drosophila melanogaster bifocal (bif), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa02790
Clone ID Related Accession (Same CDS sequence)	NM_001272527.2
Accession Version	NM_001272527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifocal, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272527.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGAAGCCAAC 
GCAGCTCCAG GTGCAATTGC AGATTTCACA GAACCGGCGA CAATCAGTAG CACTAGTCAA 
AAGAGAAACA TGATCGGTTC AGAGGAAAAG TCTGAGAAAT CTTCTATTTC CAATACCAAT 
TCCGATTCCA CTGGAGGTCA TCACTCTGTT GTTGCCGTCT CCCTTTCGCC CGATGCGGCA 
GCAACAACAA ATGTAACAGT AACACCAATA CCAAAGCAGC GATCGAGTTT ACTCAACACA 
AGAAGTCAGG AGAGGGAGAT GGTGCGATAT ATTCTAAGCG AGAGTGGAGA ACGGGATGGA 
GAGCTTGAGA GCGGCGAACA GCCGGCTGGT GTGGTGAGTA ACAGCCGGTG CGGTGAAGTT 
GAAACTGGCA CAATTGGATC GCCGTCGTCG TCAGCAAATC AAAATCCAAA CCCAAATCAT 
TTAAAAACGA AATGCAAACC GGGACAGAGC GTTGCTGAGG GCAAGCCTTC AGCTAAAGAG 
ACCATCGTCG ATAACAGCAA AAGCTGCAGC AAAACCAAGA GTATTTCCGA TAAATTGCAG 
AGCAACAAGT TTATAATTCA ACAGCAGCAG CAACAACAAC AACAACAGCA GCAACAACAG 
CAACTGTCGC CCACAAAGGT AACGGTAAAA CCGACAATGG TCGCCATGCA AGAGATGAAG 
AAGACAACCA AACAAAATGG CCAGCACCGA CATCTAGCGG GCAAAATTGG CAGCGTAGCA 
GGAGGCGATG TGGATCCACA ACATCCACCG ACGAATCCCA TACCAGATTC ATTGGACACC 
GGTGAGGATC TGAGCTACGG ACCCGGAATT GTGTCCAAGC TGCGATGCCG CTACTTGAGC 
CTGGCCCTTC GCGAGTCCCG CCAGCAGAGC AGCAAACAAC GCCTCCAACG CTCCACCAGT 
CTGAACACCC TGCTCGACCG CGACGATGAC GAGGTGGAAG TGGAGGAGCC CGAAATGACC 
AACAGCCAGG TGCGTGCCAA ATCAACACCG CCACCGATAT TGGGGGCAAA ACCGACACCG 
AAACCCAGCA GCCAGCATCA GCGTCCCGTC AGTCTGGGCG CCAATGGAGC TGGATCAGCT 
GTTAACCCGC CCTCGAACTC GGATCAGGCC CAAACGGGCG CCGTTGCCAA CGGAGGCGCA 
GGATCTGGCC AACGTTCGCG TCACTTTAAG CGCGGCAACG AGGTGATGAA GCGGGCACGT 
TCCGTGGAGG CTCTGCTCTG CGAGAAATCG CCATGGAACA GCCAGAGAAT CAGCACTGCC 
GGCGCAGCTG CAGCAGCAGC ACCTTCACCA CCTGCTCCCA AGGCCACAGC CGCTGCTCCC 
TCACCTGTTA CATCGCCCAC CTGCGTTACC ATCGAGGACA AGATCCACAA TGCCCGCGAA 
CGACTGCACA GCGGGACGGA TACGGCGCCA CCCAAGCGTC TGACCTCCAT CATCGATGAC 
ACCGAGCGGC CGCCACCGGA TCTGGTCAAG CAGACGCTCA AGATGTTTGA GGCGAGTGCT 
AATCGACGAC CGCGCGCAGC CCATCGTTCC AATGGTGTTG GCGGAGTGGC CAGCAAGGTG 
GCCAACTACA AATCGATCAT TAAGGATCAG AAGCAACCAT CATCAGCAGC GGCGACGCCA 
CCGCCGACCG GCATGGGTTT TGCCTCCTCA ACGCCGCTAA GGCATGTCCA TCCGGATATT 
ATACCCCGTC AGGTGGATTC GCCCGTATCG GCGTTGAGTG TAATGATGCG TCGCATGGAG 
CTGCAGGAGC CCGAGACGCC GGAAAGGGAG ACACGGGACG CGGAGGCCAC ACCGCAGAGC 
GAGGCGCTAA GTGAGACTGA GCGAAATGAT CGCGATGAAG GCGATGGCGA AGTCGATGAC 
GACAACCACA ACAACAACGA CGACGATCAC GACGACGGCG ACGACGGCAG CGACAACAAT 
GAGCAACGCG ATAAAATGGG CGCGGCGGCG GCAGGCGATA AGCCTAAGCC CCCAGCGGAA 
TCGGCAGCGG GAGGCGCCCA ACGATCATTC GCTGCCTCGA CGGAGAACGC GCATGTAGCC 
AGCGGTAGTA GCAGCAGTAC CAGTGCCAGT GTACGGAAGC TCAGCAACAA CAACGACTCC 
AGCAGCGGAC CAGCGGTGAC CAAACAGATC GGTGTCATCC GTCCGCTATT CAATAGCCAG 
GGAGCCGGGA GCACGCCGCT AACGAGTCGC GAGATCGAAA AGAATCGCAT CAATGAGATG 
AAGAAGTCGA CGGCCACAGA TGCCGGTGGT TCGGTATCCG GATCCGGAAC GGTTGCTGGT 
GCTGGATCAG GAACCTCGCC CACCACTAGT CTCGACAGCG TGATAAACAC CAAGGAAGCG 
GCCAGCAGCG AAACGGATGC CAGTGCCTCG CCACTGTGGA CATTGCGCAA GCTGAGGAAT 
CAGAGCCAGA CGGCCGGCGG AGGATCGGGT CCCAGTTCGA GCCTTCATTC CACAGAGAAC 
ACCTCGATGG TGTTCAACTT TTCCAAGAGC ACCAAGGAAG TGCCCGACTA CATCGAAAGC 
GACGTTGTGA TTTACAGGCG CAAGCGGGAG CTGCCAAAGC CAAATGAACC TGGCTTCGTG 
CTCCTGGGCG ATCTCTCCGT GGAGACGTCG ACGGACACGG ACTACGACGA CTACTCCATG 
TGCCCGCCAT CGCCGTGCGA TGTGGAGTTT GAGAATGCCA ACATTGTGAT CGACGGCAAG 
TCCAGCATAC GCCAGAAACC CAAAGAGTCT TCGTTCCGCG TGCAGTTCAA CGACACGCTG 
ACGTCGACGT TTGAATACCC CTCCGAGGCA TCGATGACCA TCGAGGATCC GCCGTACGCC 
GATCCCTTTG GCCATGTGAG CAAACACCAT CAGATGCTGC TCGCCGAGCA GATGCATCTG 
TTGCAGCACC AGGAGCAGCT GGAGCTGGAG CAGATGCACC AGCTGGGGCT GGCGCCGGAG 
CAGCACCATC ATGTGACCGT CGACGAGATC ATCGAGCTGC CCACATCGAC GGCGGGACAT 
GGACATGGCA TCGGACTTGG ACACAGGTCG GGGGCGGCGG GGGGCGGTGG CACGATGCTG 
GGGAATTTAC CGTTGGGTAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259456.1
CDS	330..3653
Translation	MESQKRPSLDLHTDVPAGLAAGGSGLGAAAEMSPTSGFLPDMPQWKKDLIQRRKTNVARTQAASITSPTDGSCGALAEANAAPGAIADFTEPATISSTSQKRNMIGSEEKSEKSSISNTNSDSTGGHHSVVAVSLSPDAAATTNVTVTPIPKQRSSLLNTRSQEREMVRYILSESGERDGELESGEQPAGVVSNSRCGEVETGTIGSPSSSANQNPNPNHLKTKCKPGQSVAEGKPSAKETIVDNSKSCSKTKSISDKLQSNKFIIQQQQQQQQQQQQQQQLSPTKVTVKPTMVAMQEMKKTTKQNGQHRHLAGKIGSVAGGDVDPQHPPTNPIPDSLDTGEDLSYGPGIVSKLRCRYLSLALRESRQQSSKQRLQRSTSLNTLLDRDDDEVEVEEPEMTNSQVRAKSTPPPILGAKPTPKPSSQHQRPVSLGANGAGSAVNPPSNSDQAQTGAVANGGAGSGQRSRHFKRGNEVMKRARSVEALLCEKSPWNSQRISTAGAAAAAAPSPPAPKATAAAPSPVTSPTCVTIEDKIHNARERLHSGTDTAPPKRLTSIIDDTERPPPDLVKQTLKMFEASANRRPRAAHRSNGVGGVASKVANYKSIIKDQKQPSSAAATPPPTGMGFASSTPLRHVHPDIIPRQVDSPVSALSVMMRRMELQEPETPERETRDAEATPQSEALSETERNDRDEGDGEVDDDNHNNNDDDHDDGDDGSDNNEQRDKMGAAAAGDKPKPPAESAAGGAQRSFAASTENAHVASGSSSSTSASVRKLSNNNDSSSGPAVTKQIGVIRPLFNSQGAGSTPLTSREIEKNRINEMKKSTATDAGGSVSGSGTVAGAGSGTSPTTSLDSVINTKEAASSETDASASPLWTLRKLRNQSQTAGGGSGPSSSLHSTENTSMVFNFSKSTKEVPDYIESDVVIYRRKRELPKPNEPGFVLLGDLSVETSTDTDYDDYSMCPPSPCDVEFENANIVIDGKSSIRQKPKESSFRVQFNDTLTSTFEYPSEASMTIEDPPYADPFGHVSKHHQMLLAEQMHLLQHQEQLELEQMHQLGLAPEQHHHVTVDEIIELPTSTAGHGHGIGLGHRSGAAGGGGTMLGNLPLGK

Target ORF information:

RefSeq Version	NM_001272527.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifocal (bif), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272527.2

ORF Insert Sequence:

ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGAAGCCAAC 
GCAGCTCCAG GTGCAATTGC AGATTTCACA GAACCGGCGA CAATCAGTAG CACTAGTCAA 
AAGAGAAACA TGATCGGTTC AGAGGAAAAG TCTGAGAAAT CTTCTATTTC CAATACCAAT 
TCCGATTCCA CTGGAGGTCA TCACTCTGTT GTTGCCGTCT CCCTTTCGCC CGATGCGGCA 
GCAACAACAA ATGTAACAGT AACACCAATA CCAAAGCAGC GATCGAGTTT ACTCAACACA 
AGAAGTCAGG AGAGGGAGAT GGTGCGATAT ATTCTAAGCG AGAGTGGAGA ACGGGATGGA 
GAGCTTGAGA GCGGCGAACA GCCGGCTGGT GTGGTGAGTA ACAGCCGGTG CGGTGAAGTT 
GAAACTGGCA CAATTGGATC GCCGTCGTCG TCAGCAAATC AAAATCCAAA CCCAAATCAT 
TTAAAAACGA AATGCAAACC GGGACAGAGC GTTGCTGAGG GCAAGCCTTC AGCTAAAGAG 
ACCATCGTCG ATAACAGCAA AAGCTGCAGC AAAACCAAGA GTATTTCCGA TAAATTGCAG 
AGCAACAAGT TTATAATTCA ACAGCAGCAG CAACAACAAC AACAACAGCA GCAACAACAG 
CAACTGTCGC CCACAAAGGT AACGGTAAAA CCGACAATGG TCGCCATGCA AGAGATGAAG 
AAGACAACCA AACAAAATGG CCAGCACCGA CATCTAGCGG GCAAAATTGG CAGCGTAGCA 
GGAGGCGATG TGGATCCACA ACATCCACCG ACGAATCCCA TACCAGATTC ATTGGACACC 
GGTGAGGATC TGAGCTACGG ACCCGGAATT GTGTCCAAGC TGCGATGCCG CTACTTGAGC 
CTGGCCCTTC GCGAGTCCCG CCAGCAGAGC AGCAAACAAC GCCTCCAACG CTCCACCAGT 
CTGAACACCC TGCTCGACCG CGACGATGAC GAGGTGGAAG TGGAGGAGCC CGAAATGACC 
AACAGCCAGG TGCGTGCCAA ATCAACACCG CCACCGATAT TGGGGGCAAA ACCGACACCG 
AAACCCAGCA GCCAGCATCA GCGTCCCGTC AGTCTGGGCG CCAATGGAGC TGGATCAGCT 
GTTAACCCGC CCTCGAACTC GGATCAGGCC CAAACGGGCG CCGTTGCCAA CGGAGGCGCA 
GGATCTGGCC AACGTTCGCG TCACTTTAAG CGCGGCAACG AGGTGATGAA GCGGGCACGT 
TCCGTGGAGG CTCTGCTCTG CGAGAAATCG CCATGGAACA GCCAGAGAAT CAGCACTGCC 
GGCGCAGCTG CAGCAGCAGC ACCTTCACCA CCTGCTCCCA AGGCCACAGC CGCTGCTCCC 
TCACCTGTTA CATCGCCCAC CTGCGTTACC ATCGAGGACA AGATCCACAA TGCCCGCGAA 
CGACTGCACA GCGGGACGGA TACGGCGCCA CCCAAGCGTC TGACCTCCAT CATCGATGAC 
ACCGAGCGGC CGCCACCGGA TCTGGTCAAG CAGACGCTCA AGATGTTTGA GGCGAGTGCT 
AATCGACGAC CGCGCGCAGC CCATCGTTCC AATGGTGTTG GCGGAGTGGC CAGCAAGGTG 
GCCAACTACA AATCGATCAT TAAGGATCAG AAGCAACCAT CATCAGCAGC GGCGACGCCA 
CCGCCGACCG GCATGGGTTT TGCCTCCTCA ACGCCGCTAA GGCATGTCCA TCCGGATATT 
ATACCCCGTC AGGTGGATTC GCCCGTATCG GCGTTGAGTG TAATGATGCG TCGCATGGAG 
CTGCAGGAGC CCGAGACGCC GGAAAGGGAG ACACGGGACG CGGAGGCCAC ACCGCAGAGC 
GAGGCGCTAA GTGAGACTGA GCGAAATGAT CGCGATGAAG GCGATGGCGA AGTCGATGAC 
GACAACCACA ACAACAACGA CGACGATCAC GACGACGGCG ACGACGGCAG CGACAACAAT 
GAGCAACGCG ATAAAATGGG CGCGGCGGCG GCAGGCGATA AGCCTAAGCC CCCAGCGGAA 
TCGGCAGCGG GAGGCGCCCA ACGATCATTC GCTGCCTCGA CGGAGAACGC GCATGTAGCC 
AGCGGTAGTA GCAGCAGTAC CAGTGCCAGT GTACGGAAGC TCAGCAACAA CAACGACTCC 
AGCAGCGGAC CAGCGGTGAC CAAACAGATC GGTGTCATCC GTCCGCTATT CAATAGCCAG 
GGAGCCGGGA GCACGCCGCT AACGAGTCGC GAGATCGAAA AGAATCGCAT CAATGAGATG 
AAGAAGTCGA CGGCCACAGA TGCCGGTGGT TCGGTATCCG GATCCGGAAC GGTTGCTGGT 
GCTGGATCAG GAACCTCGCC CACCACTAGT CTCGACAGCG TGATAAACAC CAAGGAAGCG 
GCCAGCAGCG AAACGGATGC CAGTGCCTCG CCACTGTGGA CATTGCGCAA GCTGAGGAAT 
CAGAGCCAGA CGGCCGGCGG AGGATCGGGT CCCAGTTCGA GCCTTCATTC CACAGAGAAC 
ACCTCGATGG TGTTCAACTT TTCCAAGAGC ACCAAGGAAG TGCCCGACTA CATCGAAAGC 
GACGTTGTGA TTTACAGGCG CAAGCGGGAG CTGCCAAAGC CAAATGAACC TGGCTTCGTG 
CTCCTGGGCG ATCTCTCCGT GGAGACGTCG ACGGACACGG ACTACGACGA CTACTCCATG 
TGCCCGCCAT CGCCGTGCGA TGTGGAGTTT GAGAATGCCA ACATTGTGAT CGACGGCAAG 
TCCAGCATAC GCCAGAAACC CAAAGAGTCT TCGTTCCGCG TGCAGTTCAA CGACACGCTG 
ACGTCGACGT TTGAATACCC CTCCGAGGCA TCGATGACCA TCGAGGATCC GCCGTACGCC 
GATCCCTTTG GCCATGTGAG CAAACACCAT CAGATGCTGC TCGCCGAGCA GATGCATCTG 
TTGCAGCACC AGGAGCAGCT GGAGCTGGAG CAGATGCACC AGCTGGGGCT GGCGCCGGAG 
CAGCACCATC ATGTGACCGT CGACGAGATC ATCGAGCTGC CCACATCGAC GGCGGGACAT 
GGACATGGCA TCGGACTTGG ACACAGGTCG GGGGCGGCGG GGGGCGGTGG CACGATGCTG 
GGGAATTTAC CGTTGGGTAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bif ( NM_001272528.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259457.1 Drosophila melanogaster bifocal (bif), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa02791
Clone ID Related Accession (Same CDS sequence)	NM_001272528.2
Accession Version	NM_001272528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifocal, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272528.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGAAGCCAAC 
GCAGCTCCAG GTGCAATTGC AGATTTCACA GAACCGGCGA CAATCAGTAG CACTAGTCAA 
AAGAGAAACA TGATCGGTTC AGAGGAAAAG TCTGAGAAAT CTTCTATTTC CAATACCAAT 
TCCGATTCCA CTGGAGGTCA TCACTCTGTT GTTGCCGTCT CCCTTTCGCC CGATGCGGCA 
GCAACAACAA ATGTAACAGT AACACCAATA CCAAAGCAGC GATCGAGTTT ACTCAACACA 
AGAAGTCAGG AGAGGGAGAT GGTGCGATAT ATTCTAAGCG AGAGTGGAGA ACGGGATGGA 
GAGCTTGAGA GCGGCGAACA GCCGGCTGGT GTGGTGAGTA ACAGCCGGTG CGGTGAAGTT 
GAAACTGGCA CAATTGGATC GCCGTCGTCG TCAGCAAATC AAAATCCAAA CCCAAATCAT 
TTAAAAACGA AATGCAAACC GGGACAGAGC GTTGCTGAGG GCAAGCCTTC AGCTAAAGAG 
ACCATCGTCG ATAACAGCAA AAGCTGCAGC AAAACCAAGA GTATTTCCGA TAAATTGCAG 
AGCAACAAGT TTATAATTCA ACAGCAGCAG CAACAACAAC AACAACAGCA GCAACAACAG 
CAACTGTCGC CCACAAAGGT AACGGTAAAA CCGACAATGG TCGCCATGCA AGAGATGAAG 
AAGACAACCA AACAAAATGG CCAGCACCGA CATCTAGCGG GCAAAATTGG CAGCGTAGCA 
GGAGGCGATG TGGATCCACA ACATCCACCG ACGAATCCCA TACCAGATTC ATTGGACACC 
GGTGAGGATC TGAGCTACGG ACCCGGAATT GTGTCCAAGC TGCGATGCCG CTACTTGAGC 
CTGGCCCTTC GCGAGTCCCG CCAGCAGAGC AGCAAACAAC GCCTCCAACG CTCCACCAGT 
CTGAACACCC TGCTCGACCG CGACGATGAC GAGGTGGAAG TGGAGGAGCC CGAAATGACC 
AACAGCCAGG TGCGTGCCAA ATCAACACCG CCACCGATAT TGGGGGCAAA ACCGACACCG 
AAACCCAGCA GCCAGCATCA GCGTCCCGTC AGTCTGGGCG CCAATGGAGC TGGATCAGCT 
GTTAACCCGC CCTCGAACTC GGATCAGGCC CAAACGGGCG CCGTTGCCAA CGGAGGCGCA 
GGATCTGGCC AACGTTCGCG TCACTTTAAG CGCGGCAACG AGGTGATGAA GCGGGCACGT 
TCCGTGGAGG CTCTGCTCTG CGAGAAATCG CCATGGAACA GCCAGAGAAT CAGCACTGCC 
GGCGCAGCTG CAGCAGCAGC ACCTTCACCA CCTGCTCCCA AGGCCACAGC CGCTGCTCCC 
TCACCTGTTA CATCGCCCAC CTGCGTTACC ATCGAGGACA AGATCCACAA TGCCCGCGAA 
CGACTGCACA GCGGGACGGA TACGGCGCCA CCCAAGCGTC TGACCTCCAT CATCGATGAC 
ACCGAGCGGC CGCCACCGGA TCTGGTCAAG CAGACGCTCA AGATGTTTGA GGCGAGTGCT 
AATCGACGAC CGCGCGCAGC CCATCGTTCC AATGGTGTTG GCGGAGTGGC CAGCAAGGTG 
GCCAACTACA AATCGATCAT TAAGGATCAG AAGCAACCAT CATCAGCAGC GGCGACGCCA 
CCGCCGACCG GCATGGGTTT TGCCTCCTCA ACGCCGCTAA GGCATGTCCA TCCGGATATT 
ATACCCCGTC AGGTGGATTC GCCCGTATCG GCGTTGAGTG TAATGATGCG TCGCATGGAG 
CTGCAGGAGC CCGAGACGCC GGAAAGGGAG ACACGGGACG CGGAGGCCAC ACCGCAGAGC 
GAGGCGCTAA GTGAGACTGA GCGAAATGAT CGCGATGAAG GCGATGGCGA AGTCGATGAC 
GACAACCACA ACAACAACGA CGACGATCAC GACGACGGCG ACGACGGCAG CGACAACAAT 
GAGCAACGCG ATAAAATGGG CGCGGCGGCG GCAGGCGATA AGCCTAAGCC CCCAGCGGAA 
TCGGCAGCGG GAGGCGCCCA ACGATCATTC GCTGCCTCGA CGGAGAACGC GCATGTAGCC 
AGCGGTAGTA GCAGCAGTAC CAGTGCCAGT GTACGGAAGC TCAGCAACAA CAACGACTCC 
AGCAGCGGAC CAGCGGTGAC CAAACAGATC GGTGTCATCC GTCCGCTATT CAATAGCCAG 
GGAGCCGGGA GCACGCCGCT AACGAGTCGC GAGATCGAAA AGAATCGCAT CAATGAGATG 
AAGAAGTCGA CGGCCACAGA TGCCGGTGGT TCGGTATCCG GATCCGGAAC GGTTGCTGGT 
GCTGGATCAG GAACCTCGCC CACCACTAGT CTCGACAGCG TGATAAACAC CAAGGAAGCG 
GCCAGCAGCG AAACGGATGC CAGTGCCTCG CCACTGTGGA CATTGCGCAA GCTGAGGAAT 
CAGAGCCAGA CGGCCGGCGG AGGATCGGGT CCCAGTTCGA GCCTTCATTC CACAGAGAAC 
ACCTCGATGG TGTTCAACTT TTCCAAGAGC ACCAAGGAAG TGCCCGACTA CATCGAAAGC 
GACGTTGTGA TTTACAGGCG CAAGCGGGAG CTGCCAAAGC CAAATGAACC TGGCTTCGTG 
CTCCTGGGCG ATCTCTCCGT GGAGACGTCG ACGGACACGG ACTACGACGA CTACTCCATG 
TGCCCGCCAT CGCCGTGCGA TGTGGAGTTT GAGAATGCCA ACATTGTGAT CGACGGCAAG 
TCCAGCATAC GCCAGAAACC CAAAGAGTCT TCGTTCCGCG TGCAGTTCAA CGACACGCTG 
ACGTCGACGT TTGAATACCC CTCCGAGGCA TCGATGACCA TCGAGGATCC GCCGTACGCC 
GATCCCTTTG GCCATGTGAG CAAACACCAT CAGATGCTGC TCGCCGAGCA GATGCATCTG 
TTGCAGCACC AGGAGCAGCT GGAGCTGGAG CAGATGCACC AGCTGGGGCT GGCGCCGGAG 
CAGCACCATC ATGTGACCGT CGACGAGATC ATCGAGCTGC CCACATCGAC GGCGGGACAT 
GGACATGGCA TCGGACTTGG ACACAGGTCG GGGGCGGCGG GGGGCGGTGG CACGATGCTG 
GGGAATTTAC CGTTGGATTA CCCAAGTTTA GGGAGGGGTA TTATCTTGCC ACAGTCGCCA 
CAACAGCAGT CAACGCAGCA GCAGGAGCAG CCAGAACCTG CAATAGCCAC ATCGGCAACA 
GTGGCAGGAT TAGCAGTGGC AATGGCAACC GAAGTGGAAA TCGAGCCACC ACTACCAGCA 
CCACCAGTCA CAGTCGCCAA GCGCCCGCAG CTCCCGGCGA AGCCAGCACA GCAGCAGCAA 
CAACAGGCGG CGCGTAGGGC CAGGCCATCG GTGCTGCTTA AGCCGCAGTA CGCCTGCTTC 
CTTCAGCACG ACGATGATGC ACCCAAATCC CAGCCAACTG CTGCTGGACG ACCGCGCAAG 
GCAGCCTCGT TTTCATTGGG CAATCAACGG CAATCGAAGG AGCAGCAGCA ACATCATCAT 
CATTACCAGC CACAGCATCA GCAGAGTAAA TATGAACGCA ACGATTGCAG TAGGAGTCGG 
GGCAGTTGCG AGAGTCCAGC TCCAGAACAC CAGCAGCGAC GTCGTCGGCC TAGTCTGCCG 
TCGTCGCTGG CTTTGTCCAA TGCCAGCTCG GCCCTGCTCC AGGCACGCAT TCCAGGCACC 
ACGCTCTACT ATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259457.1
CDS	330..4247
Translation	MESQKRPSLDLHTDVPAGLAAGGSGLGAAAEMSPTSGFLPDMPQWKKDLIQRRKTNVARTQAASITSPTDGSCGALAEANAAPGAIADFTEPATISSTSQKRNMIGSEEKSEKSSISNTNSDSTGGHHSVVAVSLSPDAAATTNVTVTPIPKQRSSLLNTRSQEREMVRYILSESGERDGELESGEQPAGVVSNSRCGEVETGTIGSPSSSANQNPNPNHLKTKCKPGQSVAEGKPSAKETIVDNSKSCSKTKSISDKLQSNKFIIQQQQQQQQQQQQQQQLSPTKVTVKPTMVAMQEMKKTTKQNGQHRHLAGKIGSVAGGDVDPQHPPTNPIPDSLDTGEDLSYGPGIVSKLRCRYLSLALRESRQQSSKQRLQRSTSLNTLLDRDDDEVEVEEPEMTNSQVRAKSTPPPILGAKPTPKPSSQHQRPVSLGANGAGSAVNPPSNSDQAQTGAVANGGAGSGQRSRHFKRGNEVMKRARSVEALLCEKSPWNSQRISTAGAAAAAAPSPPAPKATAAAPSPVTSPTCVTIEDKIHNARERLHSGTDTAPPKRLTSIIDDTERPPPDLVKQTLKMFEASANRRPRAAHRSNGVGGVASKVANYKSIIKDQKQPSSAAATPPPTGMGFASSTPLRHVHPDIIPRQVDSPVSALSVMMRRMELQEPETPERETRDAEATPQSEALSETERNDRDEGDGEVDDDNHNNNDDDHDDGDDGSDNNEQRDKMGAAAAGDKPKPPAESAAGGAQRSFAASTENAHVASGSSSSTSASVRKLSNNNDSSSGPAVTKQIGVIRPLFNSQGAGSTPLTSREIEKNRINEMKKSTATDAGGSVSGSGTVAGAGSGTSPTTSLDSVINTKEAASSETDASASPLWTLRKLRNQSQTAGGGSGPSSSLHSTENTSMVFNFSKSTKEVPDYIESDVVIYRRKRELPKPNEPGFVLLGDLSVETSTDTDYDDYSMCPPSPCDVEFENANIVIDGKSSIRQKPKESSFRVQFNDTLTSTFEYPSEASMTIEDPPYADPFGHVSKHHQMLLAEQMHLLQHQEQLELEQMHQLGLAPEQHHHVTVDEIIELPTSTAGHGHGIGLGHRSGAAGGGGTMLGNLPLDYPSLGRGIILPQSPQQQSTQQQEQPEPAIATSATVAGLAVAMATEVEIEPPLPAPPVTVAKRPQLPAKPAQQQQQQAARRARPSVLLKPQYACFLQHDDDAPKSQPTAAGRPRKAASFSLGNQRQSKEQQQHHHHYQPQHQQSKYERNDCSRSRGSCESPAPEHQQRRRRPSLPSSLALSNASSALLQARIPGTTLYYV

Target ORF information:

RefSeq Version	NM_001272528.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifocal (bif), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272528.2

ORF Insert Sequence:

ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGAAGCCAAC 
GCAGCTCCAG GTGCAATTGC AGATTTCACA GAACCGGCGA CAATCAGTAG CACTAGTCAA 
AAGAGAAACA TGATCGGTTC AGAGGAAAAG TCTGAGAAAT CTTCTATTTC CAATACCAAT 
TCCGATTCCA CTGGAGGTCA TCACTCTGTT GTTGCCGTCT CCCTTTCGCC CGATGCGGCA 
GCAACAACAA ATGTAACAGT AACACCAATA CCAAAGCAGC GATCGAGTTT ACTCAACACA 
AGAAGTCAGG AGAGGGAGAT GGTGCGATAT ATTCTAAGCG AGAGTGGAGA ACGGGATGGA 
GAGCTTGAGA GCGGCGAACA GCCGGCTGGT GTGGTGAGTA ACAGCCGGTG CGGTGAAGTT 
GAAACTGGCA CAATTGGATC GCCGTCGTCG TCAGCAAATC AAAATCCAAA CCCAAATCAT 
TTAAAAACGA AATGCAAACC GGGACAGAGC GTTGCTGAGG GCAAGCCTTC AGCTAAAGAG 
ACCATCGTCG ATAACAGCAA AAGCTGCAGC AAAACCAAGA GTATTTCCGA TAAATTGCAG 
AGCAACAAGT TTATAATTCA ACAGCAGCAG CAACAACAAC AACAACAGCA GCAACAACAG 
CAACTGTCGC CCACAAAGGT AACGGTAAAA CCGACAATGG TCGCCATGCA AGAGATGAAG 
AAGACAACCA AACAAAATGG CCAGCACCGA CATCTAGCGG GCAAAATTGG CAGCGTAGCA 
GGAGGCGATG TGGATCCACA ACATCCACCG ACGAATCCCA TACCAGATTC ATTGGACACC 
GGTGAGGATC TGAGCTACGG ACCCGGAATT GTGTCCAAGC TGCGATGCCG CTACTTGAGC 
CTGGCCCTTC GCGAGTCCCG CCAGCAGAGC AGCAAACAAC GCCTCCAACG CTCCACCAGT 
CTGAACACCC TGCTCGACCG CGACGATGAC GAGGTGGAAG TGGAGGAGCC CGAAATGACC 
AACAGCCAGG TGCGTGCCAA ATCAACACCG CCACCGATAT TGGGGGCAAA ACCGACACCG 
AAACCCAGCA GCCAGCATCA GCGTCCCGTC AGTCTGGGCG CCAATGGAGC TGGATCAGCT 
GTTAACCCGC CCTCGAACTC GGATCAGGCC CAAACGGGCG CCGTTGCCAA CGGAGGCGCA 
GGATCTGGCC AACGTTCGCG TCACTTTAAG CGCGGCAACG AGGTGATGAA GCGGGCACGT 
TCCGTGGAGG CTCTGCTCTG CGAGAAATCG CCATGGAACA GCCAGAGAAT CAGCACTGCC 
GGCGCAGCTG CAGCAGCAGC ACCTTCACCA CCTGCTCCCA AGGCCACAGC CGCTGCTCCC 
TCACCTGTTA CATCGCCCAC CTGCGTTACC ATCGAGGACA AGATCCACAA TGCCCGCGAA 
CGACTGCACA GCGGGACGGA TACGGCGCCA CCCAAGCGTC TGACCTCCAT CATCGATGAC 
ACCGAGCGGC CGCCACCGGA TCTGGTCAAG CAGACGCTCA AGATGTTTGA GGCGAGTGCT 
AATCGACGAC CGCGCGCAGC CCATCGTTCC AATGGTGTTG GCGGAGTGGC CAGCAAGGTG 
GCCAACTACA AATCGATCAT TAAGGATCAG AAGCAACCAT CATCAGCAGC GGCGACGCCA 
CCGCCGACCG GCATGGGTTT TGCCTCCTCA ACGCCGCTAA GGCATGTCCA TCCGGATATT 
ATACCCCGTC AGGTGGATTC GCCCGTATCG GCGTTGAGTG TAATGATGCG TCGCATGGAG 
CTGCAGGAGC CCGAGACGCC GGAAAGGGAG ACACGGGACG CGGAGGCCAC ACCGCAGAGC 
GAGGCGCTAA GTGAGACTGA GCGAAATGAT CGCGATGAAG GCGATGGCGA AGTCGATGAC 
GACAACCACA ACAACAACGA CGACGATCAC GACGACGGCG ACGACGGCAG CGACAACAAT 
GAGCAACGCG ATAAAATGGG CGCGGCGGCG GCAGGCGATA AGCCTAAGCC CCCAGCGGAA 
TCGGCAGCGG GAGGCGCCCA ACGATCATTC GCTGCCTCGA CGGAGAACGC GCATGTAGCC 
AGCGGTAGTA GCAGCAGTAC CAGTGCCAGT GTACGGAAGC TCAGCAACAA CAACGACTCC 
AGCAGCGGAC CAGCGGTGAC CAAACAGATC GGTGTCATCC GTCCGCTATT CAATAGCCAG 
GGAGCCGGGA GCACGCCGCT AACGAGTCGC GAGATCGAAA AGAATCGCAT CAATGAGATG 
AAGAAGTCGA CGGCCACAGA TGCCGGTGGT TCGGTATCCG GATCCGGAAC GGTTGCTGGT 
GCTGGATCAG GAACCTCGCC CACCACTAGT CTCGACAGCG TGATAAACAC CAAGGAAGCG 
GCCAGCAGCG AAACGGATGC CAGTGCCTCG CCACTGTGGA CATTGCGCAA GCTGAGGAAT 
CAGAGCCAGA CGGCCGGCGG AGGATCGGGT CCCAGTTCGA GCCTTCATTC CACAGAGAAC 
ACCTCGATGG TGTTCAACTT TTCCAAGAGC ACCAAGGAAG TGCCCGACTA CATCGAAAGC 
GACGTTGTGA TTTACAGGCG CAAGCGGGAG CTGCCAAAGC CAAATGAACC TGGCTTCGTG 
CTCCTGGGCG ATCTCTCCGT GGAGACGTCG ACGGACACGG ACTACGACGA CTACTCCATG 
TGCCCGCCAT CGCCGTGCGA TGTGGAGTTT GAGAATGCCA ACATTGTGAT CGACGGCAAG 
TCCAGCATAC GCCAGAAACC CAAAGAGTCT TCGTTCCGCG TGCAGTTCAA CGACACGCTG 
ACGTCGACGT TTGAATACCC CTCCGAGGCA TCGATGACCA TCGAGGATCC GCCGTACGCC 
GATCCCTTTG GCCATGTGAG CAAACACCAT CAGATGCTGC TCGCCGAGCA GATGCATCTG 
TTGCAGCACC AGGAGCAGCT GGAGCTGGAG CAGATGCACC AGCTGGGGCT GGCGCCGGAG 
CAGCACCATC ATGTGACCGT CGACGAGATC ATCGAGCTGC CCACATCGAC GGCGGGACAT 
GGACATGGCA TCGGACTTGG ACACAGGTCG GGGGCGGCGG GGGGCGGTGG CACGATGCTG 
GGGAATTTAC CGTTGGATTA CCCAAGTTTA GGGAGGGGTA TTATCTTGCC ACAGTCGCCA 
CAACAGCAGT CAACGCAGCA GCAGGAGCAG CCAGAACCTG CAATAGCCAC ATCGGCAACA 
GTGGCAGGAT TAGCAGTGGC AATGGCAACC GAAGTGGAAA TCGAGCCACC ACTACCAGCA 
CCACCAGTCA CAGTCGCCAA GCGCCCGCAG CTCCCGGCGA AGCCAGCACA GCAGCAGCAA 
CAACAGGCGG CGCGTAGGGC CAGGCCATCG GTGCTGCTTA AGCCGCAGTA CGCCTGCTTC 
CTTCAGCACG ACGATGATGC ACCCAAATCC CAGCCAACTG CTGCTGGACG ACCGCGCAAG 
GCAGCCTCGT TTTCATTGGG CAATCAACGG CAATCGAAGG AGCAGCAGCA ACATCATCAT 
CATTACCAGC CACAGCATCA GCAGAGTAAA TATGAACGCA ACGATTGCAG TAGGAGTCGG 
GGCAGTTGCG AGAGTCCAGC TCCAGAACAC CAGCAGCGAC GTCGTCGGCC TAGTCTGCCG 
TCGTCGCTGG CTTTGTCCAA TGCCAGCTCG GCCCTGCTCC AGGCACGCAT TCCAGGCACC 
ACGCTCTACT ATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bif ( NM_001272526.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259455.1 Drosophila melanogaster bifocal (bif), transcript variant D, mRNA.

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CloneID	OFa02792
Clone ID Related Accession (Same CDS sequence)	NM_001272526.2
Accession Version	NM_001272526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifocal, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272526.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGAAGCCAAC 
GCAGCTCCAG GTGCAATTGC AGATTTCACA GAACCGGCGA CAATCAGTAG CACTAGTCAA 
AAGAGAAACA TGATCGGTTC AGAGGAAAAG TCTGAGAAAT CTTCTATTTC CAATACCAAT 
TCCGATTCCA CTGGAGGTCA TCACTCTGTT GTTGCCGTCT CCCTTTCGCC CGATGCGGCA 
GCAACAACAA ATGTAACAGT AACACCAATA CCAAAGCAGC GATCGAGTTT ACTCAACACA 
AGAAGTCAGG AGAGGGAGAT GGTGCGATAT ATTCTAAGCG AGAGTGGAGA ACGGGATGGA 
GAGCTTGAGA GCGGCGAACA GCCGGCTGGT GTGGTGAGTA ACAGCCGGTG CGGTGAAGTT 
GAAACTGGCA CAATTGGATC GCCGTCGTCG TCAGCAAATC AAAATCCAAA CCCAAATCAT 
TTAAAAACGA AATGCAAACC GGGACAGAGC GTTGCTGAGG GCAAGCCTTC AGCTAAAGAG 
ACCATCGTCG ATAACAGCAA AAGCTGCAGC AAAACCAAGA GTATTTCCGA TAAATTGCAG 
AGCAACAAGT TTATAATTCA ACAGCAGCAG CAACAACAAC AACAACAGCA GCAACAACAG 
CAACTGTCGC CCACAAAGGT AACGGTAAAA CCGACAATGG TCGCCATGCA AGAGATGAAG 
AAGACAACCA AACAAAATGG CCAGCACCGA CATCTAGCGG GCAAAATTGG CAGCGTAGCA 
GGAGGCGATG TGGATCCACA ACATCCACCG ACGAATCCCA TACCAGATTC ATTGGACACC 
GGTGAGGATC TGAGCTACGG ACCCGGAATT GTGTCCAAGC TGCGATGCCG CTACTTGAGC 
CTGGCCCTTC GCGAGTCCCG CCAGCAGAGC AGCAAACAAC GCCTCCAACG CTCCACCAGT 
CTGAACACCC TGCTCGACCG CGACGATGAC GAGGTGGAAG TGGAGGAGCC CGAAATGACC 
AACAGCCAGG TGCGTGCCAA ATCAACACCG CCACCGATAT TGGGGGCAAA ACCGACACCG 
AAACCCAGCA GCCAGCATCA GCGTCCCGTC AGTCTGGGCG CCAATGGAGC TGGATCAGCT 
GTTAACCCGC CCTCGAACTC GGATCAGGCC CAAACGGGCG CCGTTGCCAA CGGAGGCGCA 
GGATCTGGCC AACGTTCGCG TCACTTTAAG CGCGGCAACG AGGTGATGAA GCGGGCACGT 
TCCGTGGAGG CTCTGCTCTG CGAGAAATCG CCATGGAACA GCCAGAGAAT CAGCACTGCC 
GGCGCAGCTG CAGCAGCAGC ACCTTCACCA CCTGCTCCCA AGGCCACAGC CGCTGCTCCC 
TCACCTGTTA CATCGCCCAC CTGCGTTACC ATCGAGGACA AGATCCACAA TGCCCGCGAA 
CGACTGCACA GCGGGACGGA TACGGCGCCA CCCAAGCGTC TGACCTCCAT CATCGATGAC 
ACCGAGCGGC CGCCACCGGA TCTGGTCAAG CAGACGCTCA AGATGTTTGA GGCGAGTGCT 
AATCGACGAC CGCGCGCAGC CCATCGTTCC AATGGTGTTG GCGGAGTGGC CAGCAAGGTG 
GCCAACTACA AATCGATCAT TAAGGATCAG AAGCAACCAT CATCAGCAGC GGCGACGCCA 
CCGCCGACCG GCATGGGTTT TGCCTCCTCA ACGCCGCTAA GGCATGTCCA TCCGGATATT 
ATACCCCGTC AGGTGGATTC GCCCGTATCG GCGTTGAGTG TAATGATGCG TCGCATGGAG 
CTGCAGGAGC CCGAGACGCC GGAAAGGGAG ACACGGGACG CGGAGGCCAC ACCGCAGAGC 
GAGGCGCTAA GTGAGACTGA GCGAAATGAT CGCGATGAAG GCGATGGCGA AGTCGATGAC 
GACAACCACA ACAACAACGA CGACGATCAC GACGACGGCG ACGACGGCAG CGACAACAAT 
GAGCAACGCG ATAAAATGGG CGCGGCGGCG GCAGGCGATA AGCCTAAGCC CCCAGCGGAA 
TCGGCAGCGG GAGGCGCCCA ACGATCATTC GCTGCCTCGA CGGAGAACGC GCATGTAGCC 
AGCGGTAGTA GCAGCAGTAC CAGTGCCAGT GTACGGAAGC TCAGCAACAA CAACGACTCC 
AGCAGCGGAC CAGCGGTGAC CAAACAGATC GGTGTCATCC GTCCGCTATT CAATAGCCAG 
GGAGCCGGGA GCACGCCGCT AACGAGTCGC GAGATCGAAA AGAATCGCAT CAATGAGATG 
AAGAAGTCGA CGGCCACAGA TGCCGGTGGT TCGGTATCCG GATCCGGAAC GGTTGCTGGT 
GCTGGATCAG GAACCTCGCC CACCACTAGT CTCGACAGCG TGATAAACAC CAAGGAAGCG 
GCCAGCAGCG AAACGGATGC CAGTGCCTCG CCACTGTGGA CATTGCGCAA GCTGAGGAAT 
CAGAGCCAGA CGGCCGGCGG AGGATCGGGT CCCAGTTCGA GCCTTCATTC CACAGAGAAC 
ACCTCGATGG TGTTCAACTT TTCCAAGAGC ACCAAGGAAG TGCCCGACTA CATCGAAAGC 
GACGTTGTGA TTTACAGGCG CAAGCGGGAG CTGCCAAAGT TCCGCGTGCA GTTCAACGAC 
ACGCTGACGT CGACGTTTGA ATACCCCTCC GAGGCATCGA TGACCATCGA GGATCCGCCG 
TACGCCGATC CCTTTGGCCA TGTGAGCAAA CACCATCAGA TGCTGCTCGC CGAGCAGATG 
CATCTGTTGC AGCACCAGGA GCAGCTGGAG CTGGAGCAGA TGCACCAGCT GGGGCTGGCG 
CCGGAGCAGC ACCATCATGT GACCGTCGAC GAGATCATCG AGCTGCCCAC ATCGACGGCG 
GGACATGGAC ATGGCATCGG ACTTGGACAC AGGTCGGGGG CGGCGGGGGG CGGTGGCACG 
ATGCTGGGGA ATTTACCGTT GGGCTCCACG GCTCTGGGCT TCTACACGCC CATGAAGGGC 
ACGGCGATGG ACAACCTATT CCAGCTGGGT GTCACCCGAT ACGCCCTGCC CGAGAGCAGC 
AGCAGTGGGA GCAGCAATGG CAGCCACAGT CCAGGCAGCA ACGGGAGCAG TCCAGCCGGA 
GCTGCGGGCA GCCGGTTGAT GTTCAACGGG AATGGCAGCA TTATGGGCAT CGGCGAGGGA 
CTGATCAAGG AGGGCGACTT GGCGGCAACG GAAGCAGGAC CTGGAACTGC AGATGGAGCA 
GGTGCCTCCA AGAAGGGTTC AGCTGTGGAC GAGGATGATT ACCATCTGAC GGCGACGCCG 
GGCGCCGAAG TTGTGTACAG CGAGGGTACA CAGAAAACGG ATTTGCTATA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259455.1
CDS	330..3863
Translation	MESQKRPSLDLHTDVPAGLAAGGSGLGAAAEMSPTSGFLPDMPQWKKDLIQRRKTNVARTQAASITSPTDGSCGALAEANAAPGAIADFTEPATISSTSQKRNMIGSEEKSEKSSISNTNSDSTGGHHSVVAVSLSPDAAATTNVTVTPIPKQRSSLLNTRSQEREMVRYILSESGERDGELESGEQPAGVVSNSRCGEVETGTIGSPSSSANQNPNPNHLKTKCKPGQSVAEGKPSAKETIVDNSKSCSKTKSISDKLQSNKFIIQQQQQQQQQQQQQQQLSPTKVTVKPTMVAMQEMKKTTKQNGQHRHLAGKIGSVAGGDVDPQHPPTNPIPDSLDTGEDLSYGPGIVSKLRCRYLSLALRESRQQSSKQRLQRSTSLNTLLDRDDDEVEVEEPEMTNSQVRAKSTPPPILGAKPTPKPSSQHQRPVSLGANGAGSAVNPPSNSDQAQTGAVANGGAGSGQRSRHFKRGNEVMKRARSVEALLCEKSPWNSQRISTAGAAAAAAPSPPAPKATAAAPSPVTSPTCVTIEDKIHNARERLHSGTDTAPPKRLTSIIDDTERPPPDLVKQTLKMFEASANRRPRAAHRSNGVGGVASKVANYKSIIKDQKQPSSAAATPPPTGMGFASSTPLRHVHPDIIPRQVDSPVSALSVMMRRMELQEPETPERETRDAEATPQSEALSETERNDRDEGDGEVDDDNHNNNDDDHDDGDDGSDNNEQRDKMGAAAAGDKPKPPAESAAGGAQRSFAASTENAHVASGSSSSTSASVRKLSNNNDSSSGPAVTKQIGVIRPLFNSQGAGSTPLTSREIEKNRINEMKKSTATDAGGSVSGSGTVAGAGSGTSPTTSLDSVINTKEAASSETDASASPLWTLRKLRNQSQTAGGGSGPSSSLHSTENTSMVFNFSKSTKEVPDYIESDVVIYRRKRELPKFRVQFNDTLTSTFEYPSEASMTIEDPPYADPFGHVSKHHQMLLAEQMHLLQHQEQLELEQMHQLGLAPEQHHHVTVDEIIELPTSTAGHGHGIGLGHRSGAAGGGGTMLGNLPLGSTALGFYTPMKGTAMDNLFQLGVTRYALPESSSSGSSNGSHSPGSNGSSPAGAAGSRLMFNGNGSIMGIGEGLIKEGDLAATEAGPGTADGAGASKKGSAVDEDDYHLTATPGAEVVYSEGTQKTDLLY

Target ORF information:

RefSeq Version	NM_001272526.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifocal (bif), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272526.2

ORF Insert Sequence:

ATGGAGTCAC AGAAGCGGCC TTCGTTGGAC TTACACACGG ATGTGCCAGC AGGATTAGCA 
GCTGGAGGAT CTGGCCTGGG AGCTGCAGCT GAGATGTCGC CCACTTCCGG TTTCCTGCCG 
GACATGCCGC AGTGGAAGAA GGACCTCATC CAGCGCCGGA AAACGAACGT GGCCCGCACC 
CAGGCGGCGT CCATCACCTC GCCCACCGAT GGCAGTTGTG GGGCTTTGGC AGAAGCCAAC 
GCAGCTCCAG GTGCAATTGC AGATTTCACA GAACCGGCGA CAATCAGTAG CACTAGTCAA 
AAGAGAAACA TGATCGGTTC AGAGGAAAAG TCTGAGAAAT CTTCTATTTC CAATACCAAT 
TCCGATTCCA CTGGAGGTCA TCACTCTGTT GTTGCCGTCT CCCTTTCGCC CGATGCGGCA 
GCAACAACAA ATGTAACAGT AACACCAATA CCAAAGCAGC GATCGAGTTT ACTCAACACA 
AGAAGTCAGG AGAGGGAGAT GGTGCGATAT ATTCTAAGCG AGAGTGGAGA ACGGGATGGA 
GAGCTTGAGA GCGGCGAACA GCCGGCTGGT GTGGTGAGTA ACAGCCGGTG CGGTGAAGTT 
GAAACTGGCA CAATTGGATC GCCGTCGTCG TCAGCAAATC AAAATCCAAA CCCAAATCAT 
TTAAAAACGA AATGCAAACC GGGACAGAGC GTTGCTGAGG GCAAGCCTTC AGCTAAAGAG 
ACCATCGTCG ATAACAGCAA AAGCTGCAGC AAAACCAAGA GTATTTCCGA TAAATTGCAG 
AGCAACAAGT TTATAATTCA ACAGCAGCAG CAACAACAAC AACAACAGCA GCAACAACAG 
CAACTGTCGC CCACAAAGGT AACGGTAAAA CCGACAATGG TCGCCATGCA AGAGATGAAG 
AAGACAACCA AACAAAATGG CCAGCACCGA CATCTAGCGG GCAAAATTGG CAGCGTAGCA 
GGAGGCGATG TGGATCCACA ACATCCACCG ACGAATCCCA TACCAGATTC ATTGGACACC 
GGTGAGGATC TGAGCTACGG ACCCGGAATT GTGTCCAAGC TGCGATGCCG CTACTTGAGC 
CTGGCCCTTC GCGAGTCCCG CCAGCAGAGC AGCAAACAAC GCCTCCAACG CTCCACCAGT 
CTGAACACCC TGCTCGACCG CGACGATGAC GAGGTGGAAG TGGAGGAGCC CGAAATGACC 
AACAGCCAGG TGCGTGCCAA ATCAACACCG CCACCGATAT TGGGGGCAAA ACCGACACCG 
AAACCCAGCA GCCAGCATCA GCGTCCCGTC AGTCTGGGCG CCAATGGAGC TGGATCAGCT 
GTTAACCCGC CCTCGAACTC GGATCAGGCC CAAACGGGCG CCGTTGCCAA CGGAGGCGCA 
GGATCTGGCC AACGTTCGCG TCACTTTAAG CGCGGCAACG AGGTGATGAA GCGGGCACGT 
TCCGTGGAGG CTCTGCTCTG CGAGAAATCG CCATGGAACA GCCAGAGAAT CAGCACTGCC 
GGCGCAGCTG CAGCAGCAGC ACCTTCACCA CCTGCTCCCA AGGCCACAGC CGCTGCTCCC 
TCACCTGTTA CATCGCCCAC CTGCGTTACC ATCGAGGACA AGATCCACAA TGCCCGCGAA 
CGACTGCACA GCGGGACGGA TACGGCGCCA CCCAAGCGTC TGACCTCCAT CATCGATGAC 
ACCGAGCGGC CGCCACCGGA TCTGGTCAAG CAGACGCTCA AGATGTTTGA GGCGAGTGCT 
AATCGACGAC CGCGCGCAGC CCATCGTTCC AATGGTGTTG GCGGAGTGGC CAGCAAGGTG 
GCCAACTACA AATCGATCAT TAAGGATCAG AAGCAACCAT CATCAGCAGC GGCGACGCCA 
CCGCCGACCG GCATGGGTTT TGCCTCCTCA ACGCCGCTAA GGCATGTCCA TCCGGATATT 
ATACCCCGTC AGGTGGATTC GCCCGTATCG GCGTTGAGTG TAATGATGCG TCGCATGGAG 
CTGCAGGAGC CCGAGACGCC GGAAAGGGAG ACACGGGACG CGGAGGCCAC ACCGCAGAGC 
GAGGCGCTAA GTGAGACTGA GCGAAATGAT CGCGATGAAG GCGATGGCGA AGTCGATGAC 
GACAACCACA ACAACAACGA CGACGATCAC GACGACGGCG ACGACGGCAG CGACAACAAT 
GAGCAACGCG ATAAAATGGG CGCGGCGGCG GCAGGCGATA AGCCTAAGCC CCCAGCGGAA 
TCGGCAGCGG GAGGCGCCCA ACGATCATTC GCTGCCTCGA CGGAGAACGC GCATGTAGCC 
AGCGGTAGTA GCAGCAGTAC CAGTGCCAGT GTACGGAAGC TCAGCAACAA CAACGACTCC 
AGCAGCGGAC CAGCGGTGAC CAAACAGATC GGTGTCATCC GTCCGCTATT CAATAGCCAG 
GGAGCCGGGA GCACGCCGCT AACGAGTCGC GAGATCGAAA AGAATCGCAT CAATGAGATG 
AAGAAGTCGA CGGCCACAGA TGCCGGTGGT TCGGTATCCG GATCCGGAAC GGTTGCTGGT 
GCTGGATCAG GAACCTCGCC CACCACTAGT CTCGACAGCG TGATAAACAC CAAGGAAGCG 
GCCAGCAGCG AAACGGATGC CAGTGCCTCG CCACTGTGGA CATTGCGCAA GCTGAGGAAT 
CAGAGCCAGA CGGCCGGCGG AGGATCGGGT CCCAGTTCGA GCCTTCATTC CACAGAGAAC 
ACCTCGATGG TGTTCAACTT TTCCAAGAGC ACCAAGGAAG TGCCCGACTA CATCGAAAGC 
GACGTTGTGA TTTACAGGCG CAAGCGGGAG CTGCCAAAGT TCCGCGTGCA GTTCAACGAC 
ACGCTGACGT CGACGTTTGA ATACCCCTCC GAGGCATCGA TGACCATCGA GGATCCGCCG 
TACGCCGATC CCTTTGGCCA TGTGAGCAAA CACCATCAGA TGCTGCTCGC CGAGCAGATG 
CATCTGTTGC AGCACCAGGA GCAGCTGGAG CTGGAGCAGA TGCACCAGCT GGGGCTGGCG 
CCGGAGCAGC ACCATCATGT GACCGTCGAC GAGATCATCG AGCTGCCCAC ATCGACGGCG 
GGACATGGAC ATGGCATCGG ACTTGGACAC AGGTCGGGGG CGGCGGGGGG CGGTGGCACG 
ATGCTGGGGA ATTTACCGTT GGGCTCCACG GCTCTGGGCT TCTACACGCC CATGAAGGGC 
ACGGCGATGG ACAACCTATT CCAGCTGGGT GTCACCCGAT ACGCCCTGCC CGAGAGCAGC 
AGCAGTGGGA GCAGCAATGG CAGCCACAGT CCAGGCAGCA ACGGGAGCAG TCCAGCCGGA 
GCTGCGGGCA GCCGGTTGAT GTTCAACGGG AATGGCAGCA TTATGGGCAT CGGCGAGGGA 
CTGATCAAGG AGGGCGACTT GGCGGCAACG GAAGCAGGAC CTGGAACTGC AGATGGAGCA 
GGTGCCTCCA AGAAGGGTTC AGCTGTGGAC GAGGATGATT ACCATCTGAC GGCGACGCCG 
GGCGCCGAAG TTGTGTACAG CGAGGGTACA CAGAAAACGG ATTTGCTATA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bif cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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