Trx-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trx-2
Entrez Gene ID	34281
Full Name	thioredoxin-2
Synonyms	31884,CG31884,CG3864,DTrx-2,DmTrx-2,DmelTrx-2,Dmel\CG31884,Trx,anon-WO0118547.188,dmtrx-2
General protein information	Preferred Names thioredoxin-2 Names CG31884 gene product from transcript CG31884-RB CG31884-PA CG31884-PB Thioredoxin-2 Trx-2-PA Trx-2-PB thioredoxin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30C1-30C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_078802.4	NP_523526.1	thioredoxin-2, isoform A
NM_164863.3	NP_723475.1	thioredoxin-2, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC474798	XP_532029.2
Gallus gallus (chicken)	TXN	NP_990784.1
Xenopus tropicalis (tropical clawed frog)	LOC100170420	NP_001123670.1
Drosophila melanogaster (fruit fly)	Trx-2	NP_523526.1
Saccharomyces cerevisiae (baker's yeast)	TRX3	NP_010006.1
Oryza sativa (rice)	Os07g0190800	NP_001059090.1
Bos taurus (cattle)	TXN	NP_776393.1
Kluyveromyces lactis	KLLA0F10351g	XP_455551.1
Neurospora crassa	NCU06556	XP_962263.2
Homo sapiens (human)	TXN	NP_003320.2
Pan troglodytes (chimpanzee)	TXN	XP_003339222.1
	TXN	XP_001083928.1
Danio rerio (zebrafish)	zgc:56493	NP_956317.1
Anopheles gambiae (African malaria mosquito)	TRX1	XP_318603.3
Schizosaccharomyces pombe (fission yeast)	trx1	NP_593852.1
Magnaporthe oryzae (rice blast fungus)	MGG_04236	XP_003719578.1
Arabidopsis thaliana (thale cress)	TRX2	NP_198811.1
Oryza sativa (rice)	Os03g0800700	NP_001051587.1
Mus musculus (house mouse)	Txn1	NP_035790.1
Rattus norvegicus (Norway rat)	Txn1	NP_446252.1
Danio rerio (zebrafish)	txn	NP_001002461.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first demonstration of biochemical and physiological consequences caused by loss of Trx-2 in Drosophila.
Title: Loss of Trx-2 enhances oxidative stress-dependent phenotypes in Drosophila.

Drosophila thioredoxin can function as an anti-aging agent and as a suppressor of Parkin-associated endothelin receptor-like receptor - and poly-glutamine-induced neurotoxicity
Title: Thioredoxin suppresses Parkin-associated endothelin receptor-like receptor-induced neurotoxicity and extends longevity in Drosophila.

Trx-2 affects lifespan in Drosophila. Mutants have a decreased lifespan.
Title: Thioredoxin-2 affects lifespan and oxidative stress in Drosophila.

gene for thioredoxin (DmTrx-2) was cloned and expressed, and the protein characterized
Title: Thioredoxin-2 but not thioredoxin-1 is a substrate of thioredoxin peroxidase-1 from Drosophila melanogaster: isolation and characterization of a second thioredoxin in D. Melanogaster and evidence for distinct biological functions of Trx-1 and Trx-2.

The following Trx-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trx-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14213	NM_078802.4 Latest version!	Drosophila melanogaster thioredoxin-2 (Trx-2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa14213	NM_164863.3 Latest version!	Drosophila melanogaster thioredoxin-2 (Trx-2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trx-2 ( NM_078802.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523526.1 Drosophila melanogaster thioredoxin-2 (Trx-2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14213
Clone ID Related Accession (Same CDS sequence)	NM_078802.4 , NM_164863.3
Accession Version	NM_078802.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thioredoxin-2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078802.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTACC AGGTGAAAGA TAAGGCCGAT CTCGATGGAC AGCTGACCAA GGCATCCGGC 
AAGCTGGTGG TGCTGGATTT CTTCGCCACT TGGTGCGGAC CCTGCAAGAT GATCTCGCCC 
AAACTGGTTG AGCTTTCCAC GCAGTTCGCC GACAACGTCG TCGTCCTGAA GGTCGATGTG 
GACGAATGCG AAGACATTGC AATGGAATAC AACATCTCCA GCATGCCCAC CTTCGTGTTC 
CTCAAGAACG GCGTCAAGGT CGAAGAGTTC GCCGGAGCCA ACGCCAAGCG TCTGGAGGAT 
GTCATCAAGG CCAATATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523526.1
CDS	104..424
Translation	MVYQVKDKADLDGQLTKASGKLVVLDFFATWCGPCKMISPKLVELSTQFADNVVVLKVDVDECEDIAMEYNISSMPTFVFLKNGVKVEEFAGANAKRLEDVIKANI

Target ORF information:

RefSeq Version	NM_078802.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thioredoxin-2 (Trx-2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078802.4

ORF Insert Sequence:

ATGGTGTACC AGGTGAAAGA TAAGGCCGAT CTCGATGGAC AGCTGACCAA GGCATCCGGC 
AAGCTGGTGG TGCTGGATTT CTTCGCCACT TGGTGCGGAC CCTGCAAGAT GATCTCGCCC 
AAACTGGTTG AGCTTTCCAC GCAGTTCGCC GACAACGTCG TCGTCCTGAA GGTCGATGTG 
GACGAATGCG AAGACATTGC AATGGAATAC AACATCTCCA GCATGCCCAC CTTCGTGTTC 
CTCAAGAACG GCGTCAAGGT CGAAGAGTTC GCCGGAGCCA ACGCCAAGCG TCTGGAGGAT 
GTCATCAAGG CCAATATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trx-2 ( NM_164863.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723475.1 Drosophila melanogaster thioredoxin-2 (Trx-2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14213
Clone ID Related Accession (Same CDS sequence)	NM_078802.4 , NM_164863.3
Accession Version	NM_164863.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thioredoxin-2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164863.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTACC AGGTGAAAGA TAAGGCCGAT CTCGATGGAC AGCTGACCAA GGCATCCGGC 
AAGCTGGTGG TGCTGGATTT CTTCGCCACT TGGTGCGGAC CCTGCAAGAT GATCTCGCCC 
AAACTGGTTG AGCTTTCCAC GCAGTTCGCC GACAACGTCG TCGTCCTGAA GGTCGATGTG 
GACGAATGCG AAGACATTGC AATGGAATAC AACATCTCCA GCATGCCCAC CTTCGTGTTC 
CTCAAGAACG GCGTCAAGGT CGAAGAGTTC GCCGGAGCCA ACGCCAAGCG TCTGGAGGAT 
GTCATCAAGG CCAATATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723475.1
CDS	295..615
Translation	MVYQVKDKADLDGQLTKASGKLVVLDFFATWCGPCKMISPKLVELSTQFADNVVVLKVDVDECEDIAMEYNISSMPTFVFLKNGVKVEEFAGANAKRLEDVIKANI

Target ORF information:

RefSeq Version	NM_164863.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thioredoxin-2 (Trx-2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164863.3

ORF Insert Sequence:

ATGGTGTACC AGGTGAAAGA TAAGGCCGAT CTCGATGGAC AGCTGACCAA GGCATCCGGC 
AAGCTGGTGG TGCTGGATTT CTTCGCCACT TGGTGCGGAC CCTGCAAGAT GATCTCGCCC 
AAACTGGTTG AGCTTTCCAC GCAGTTCGCC GACAACGTCG TCGTCCTGAA GGTCGATGTG 
GACGAATGCG AAGACATTGC AATGGAATAC AACATCTCCA GCATGCCCAC CTTCGTGTTC 
CTCAAGAACG GCGTCAAGGT CGAAGAGTTC GCCGGAGCCA ACGCCAAGCG TCTGGAGGAT 
GTCATCAAGG CCAATATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Trx-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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