ppk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppk
Entrez Gene ID	34843
Full Name	pickpocket
Synonyms	BG:DS06238.1,CG3478,Dmel\CG3478,PPK,PPK1,dmdNaC1,dmdNaCl,mdNaC1,pkt,ppk1
General protein information	Preferred Names pickpocket Names CG3478 gene product from transcript CG3478-RA CG3478-PA Pickpocket1 drifter multidendritic neurons sodium channel 1 pickpocket 1 ppk-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35B1-35B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057884.5	NP_477232.1	pickpocket

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

water,salt and pheromone sensing neurons that express members of the pickpocket families.
Title: Molecular neurobiology of Drosophila taste.

Balboa (also known as CG8546, ppk26) and PPK physically interact and likely form a functional ion channel. Both genes are functionally required for mechanical nociception and show very specific enrichment and expression in the class IV nociceptor neurons.
Title: Balboa binds to pickpocket in vivo and is required for mechanical nociception in Drosophila larvae.

Ppk26, a member of the trimeric degenerin/epithelial sodium channel (DEG/ENaC) family, is necessary for proper locomotion behavior in Drosophila larvae in a mutually dependent fashion with coexpressed Ppk1.
Title: Identification of Ppk26, a DEG/ENaC Channel Functioning with Ppk1 in a Mutually Dependent Manner to Guide Locomotion Behavior in Drosophila.

Deficiency of the mechanosensory ion channel PPK1 gene or RNAi knockdown of its expression in the PENS result in a similar increase in food intake, which can be rescued by expression of wild-type PPK1 in the same neurons.
Title: Regulation of food intake by mechanosensory ion channels in enteric neurons.

Presynaptic calcium imaging experiments support a model in which a PPK11- and PPK16-containing DEG/ENaC channel modulates presynaptic membrane voltage .
Title: A presynaptic ENaC channel drives homeostatic plasticity.

The following ppk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16783	NM_057884.5 Latest version!	Drosophila melanogaster pickpocket (ppk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppk ( NM_057884.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477232.1 Drosophila melanogaster pickpocket (ppk), mRNA.

Price & Availability↑

CloneID	OFa16783
Clone ID Related Accession (Same CDS sequence)	NM_057884.5
Accession Version	NM_057884.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pickpocket
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057884.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGA TCAGGGAGGA TGAGGAGGAA AAGAAGTCGG GCATCAGCAT TCTGCCCGGT 
CCGGAACTAT TGGCGCTTCC CGGCTTCGAT ACCCGGGCCA GTATTGCATC GGCTGCATTA 
AGTGATGTTC CAAGTGATGT GATCATTAAG TCTCGCATTC GATACGGCAG TCCTCTGTCA 
GCCTGCAAAG GATTACTTTT GGAGTATGCG AAGAGCACGA CCATTCACGG CATACGCTAC 
ATCTTCGAGG TTCACAGACC GATCTATGAA AAACTTTATT GGCTTTTCTT CACGTGCATT 
TCCGTTTACT TTGCCGTTTC ACTTATCTGG GACACCTATC TAAAGTGGCA GGAATCGCCG 
GTTATTCTAG GTTTCGACGA GACTTTGGTG CCAGTGCATA AGATACCGTT TCCCACAATT 
ACCATTTGTC CGGAAATCAA AATGGAACGC AATGTCTTTG ACTATACTAA CGTATCGCGA 
CAACTTTGGG AGGAGTACAA ACAGAATGGC AATATCAGCG ATTTGGATGA TGAGGACCTG 
GCTCGAATGG CGGTTGCAAT GCACATCTGT GATTCGGAAG TGGTGCAGCG GTTTACTCCA 
TTGCTATCGC AGCTAAATCC GCCGAACGTG GACGTAACCC AAACTCTGAT TGACTTGAGT 
ATATCGAAGA ATGAGACGGG TCCGTTTTGC AAGTGGAATG GTCGCTTTTA CTTCTGTGAC 
AAGATCTTCG ACTTTGTGGC CACCGACGAG GGTATCTGCT ATCAATTCAA TGGACTGCGA 
CCCAAGGACA TTTATCGTGA CGAGAAGTTC ATCAGCTATG TGGATCCGGA TGTGGTGGAC 
TTCAACAAGT ACTTCGATGT GGATTTGCCT CCGTGGAACA ACATAACTGG CAATTGGTCA 
CTGGACACTG GATTCGTGGA CCAGGGTCAG AATGCGTATC CCCAACGGAC GGTGTTCTCA 
TCGGTGAAAA ATGGGTTCTT CGCCTTTCTG CAGGGTTTGC AGCATAATTT CGACTACGAT 
TGTCGCAGTT TCAAACAGGG CTATAAGGTC TTTCTCAACT CCCCGGAGAG TGTGCCGCTG 
ACGACGGGCA ATTATATCCT AGTCCCTCAT GGCGATGAGG TCCTGGTCAG TGTGCTGCCA 
GCATATGTCG TGTCCACTGA CAATTTGCAC GAAATTACAC CCGAAAAACG CCAATGTCTG 
TTCGATGATG AGCGATCGCT ACGATTCTTC CGTTCCTATT CCCAGAGCAA CTGCCAAACC 
GAATGTCTGG CCAACTATAC GGTTTCCAAA TGCGGTTGTG CCAAATTCTG GATGCCCAAA 
CCTCTTGGCA CTCCCGTTTG TGGCCTCAAG GACATCAATT GCTACACATC GGCCCAGGAT 
GAACTCTACA CGCTGATGCA AAACCAAACG ATGGCCAAGA GTATTGATGA AAGTGTGGAC 
ATCACCTGCA ACTGCATGCC AGCCTGCACT TCGCTGGAAT ATAATTTCGA GATATCTAGG 
GCCAAATACG ATGTGGCTAA AACCATTCGA GCCTTCCGCG AAGAATACGA GCATACGGAC 
GCAATTGGAT CCCGACTCTC CGTCTACTTT AAGGAGCATC AGTTTACGGC CATTAAACGC 
ACTATTCTTT TCGGGGTATC CACATTGATT TCCAACTGCG GCGGTATCTG CGGATTGTTT 
ATGGGAATCT CATGTCTGAG TTTCCTGGAG CTAATCTACT TCTTCTGCAT GAGAATCTGT 
GGCAGCTGCC GCGATCGCAG GAAGCACAAG ATACAACAGC AGAACTCAGT GGATTTACCA 
GAGGAAAAAT CTGAGAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477232.1
CDS	39..1859
Translation	MAEIREDEEEKKSGISILPGPELLALPGFDTRASIASAALSDVPSDVIIKSRIRYGSPLSACKGLLLEYAKSTTIHGIRYIFEVHRPIYEKLYWLFFTCISVYFAVSLIWDTYLKWQESPVILGFDETLVPVHKIPFPTITICPEIKMERNVFDYTNVSRQLWEEYKQNGNISDLDDEDLARMAVAMHICDSEVVQRFTPLLSQLNPPNVDVTQTLIDLSISKNETGPFCKWNGRFYFCDKIFDFVATDEGICYQFNGLRPKDIYRDEKFISYVDPDVVDFNKYFDVDLPPWNNITGNWSLDTGFVDQGQNAYPQRTVFSSVKNGFFAFLQGLQHNFDYDCRSFKQGYKVFLNSPESVPLTTGNYILVPHGDEVLVSVLPAYVVSTDNLHEITPEKRQCLFDDERSLRFFRSYSQSNCQTECLANYTVSKCGCAKFWMPKPLGTPVCGLKDINCYTSAQDELYTLMQNQTMAKSIDESVDITCNCMPACTSLEYNFEISRAKYDVAKTIRAFREEYEHTDAIGSRLSVYFKEHQFTAIKRTILFGVSTLISNCGGICGLFMGISCLSFLELIYFFCMRICGSCRDRRKHKIQQQNSVDLPEEKSEN

Target ORF information:

RefSeq Version	NM_057884.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pickpocket (ppk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057884.5

ORF Insert Sequence:

ATGGCCGAGA TCAGGGAGGA TGAGGAGGAA AAGAAGTCGG GCATCAGCAT TCTGCCCGGT 
CCGGAACTAT TGGCGCTTCC CGGCTTCGAT ACCCGGGCCA GTATTGCATC GGCTGCATTA 
AGTGATGTTC CAAGTGATGT GATCATTAAG TCTCGCATTC GATACGGCAG TCCTCTGTCA 
GCCTGCAAAG GATTACTTTT GGAGTATGCG AAGAGCACGA CCATTCACGG CATACGCTAC 
ATCTTCGAGG TTCACAGACC GATCTATGAA AAACTTTATT GGCTTTTCTT CACGTGCATT 
TCCGTTTACT TTGCCGTTTC ACTTATCTGG GACACCTATC TAAAGTGGCA GGAATCGCCG 
GTTATTCTAG GTTTCGACGA GACTTTGGTG CCAGTGCATA AGATACCGTT TCCCACAATT 
ACCATTTGTC CGGAAATCAA AATGGAACGC AATGTCTTTG ACTATACTAA CGTATCGCGA 
CAACTTTGGG AGGAGTACAA ACAGAATGGC AATATCAGCG ATTTGGATGA TGAGGACCTG 
GCTCGAATGG CGGTTGCAAT GCACATCTGT GATTCGGAAG TGGTGCAGCG GTTTACTCCA 
TTGCTATCGC AGCTAAATCC GCCGAACGTG GACGTAACCC AAACTCTGAT TGACTTGAGT 
ATATCGAAGA ATGAGACGGG TCCGTTTTGC AAGTGGAATG GTCGCTTTTA CTTCTGTGAC 
AAGATCTTCG ACTTTGTGGC CACCGACGAG GGTATCTGCT ATCAATTCAA TGGACTGCGA 
CCCAAGGACA TTTATCGTGA CGAGAAGTTC ATCAGCTATG TGGATCCGGA TGTGGTGGAC 
TTCAACAAGT ACTTCGATGT GGATTTGCCT CCGTGGAACA ACATAACTGG CAATTGGTCA 
CTGGACACTG GATTCGTGGA CCAGGGTCAG AATGCGTATC CCCAACGGAC GGTGTTCTCA 
TCGGTGAAAA ATGGGTTCTT CGCCTTTCTG CAGGGTTTGC AGCATAATTT CGACTACGAT 
TGTCGCAGTT TCAAACAGGG CTATAAGGTC TTTCTCAACT CCCCGGAGAG TGTGCCGCTG 
ACGACGGGCA ATTATATCCT AGTCCCTCAT GGCGATGAGG TCCTGGTCAG TGTGCTGCCA 
GCATATGTCG TGTCCACTGA CAATTTGCAC GAAATTACAC CCGAAAAACG CCAATGTCTG 
TTCGATGATG AGCGATCGCT ACGATTCTTC CGTTCCTATT CCCAGAGCAA CTGCCAAACC 
GAATGTCTGG CCAACTATAC GGTTTCCAAA TGCGGTTGTG CCAAATTCTG GATGCCCAAA 
CCTCTTGGCA CTCCCGTTTG TGGCCTCAAG GACATCAATT GCTACACATC GGCCCAGGAT 
GAACTCTACA CGCTGATGCA AAACCAAACG ATGGCCAAGA GTATTGATGA AAGTGTGGAC 
ATCACCTGCA ACTGCATGCC AGCCTGCACT TCGCTGGAAT ATAATTTCGA GATATCTAGG 
GCCAAATACG ATGTGGCTAA AACCATTCGA GCCTTCCGCG AAGAATACGA GCATACGGAC 
GCAATTGGAT CCCGACTCTC CGTCTACTTT AAGGAGCATC AGTTTACGGC CATTAAACGC 
ACTATTCTTT TCGGGGTATC CACATTGATT TCCAACTGCG GCGGTATCTG CGGATTGTTT 
ATGGGAATCT CATGTCTGAG TTTCCTGGAG CTAATCTACT TCTTCTGCAT GAGAATCTGT 
GGCAGCTGCC GCGATCGCAG GAAGCACAAG ATACAACAGC AGAACTCAGT GGATTTACCA 
GAGGAAAAAT CTGAGAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ppk cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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