BubR1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	BubR1
Entrez Gene ID	35522
Full Name	Bub1-related kinase
Synonyms	31/13,BUB1,BUBR1,BcDNA:LD23835,Bub1,CG 7838,CG7838,DmBUB1,DmBubRI,Dmbub1,Dmel\CG7838,bub1,bubR1,bubr1,dBUB1,l(2)k03113,l(2)k06109,mcl(2)Z1525
General protein information	Preferred Names Bub1-related kinase Names CG7838 gene product from transcript CG7838-RA BubR1-PA CG7838-PA bub related one
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42A5-42A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_058034.4	NP_477382.1	Bub1-related kinase

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	BubR1	NP_477382.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011124	XP_309522.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Bub3-BubR1 complex on broken DNA inhibits the APC/C locally via the sequestration of Cdc20.
Title: Bub3-BubR1-dependent sequestration of Cdc20Fizzy at DNA breaks facilitates the correct segregation of broken chromosomes.

a model in which Polo controls Mps1-dependent BubR1 phosphorylation to promote Cdc20 kinetochore recruitment and sustained SAC function.
Title: Drosophila Polo regulates the spindle assembly checkpoint through Mps1-dependent BubR1 phosphorylation.

Data show that the Mad2 kinetochore dimerization recruitment mechanism is conserved and that the recruitment of Cdc20 to kinetochores in Drosophila requires BubR1 but not Mad2.
Title: Recruitment of Cdc20 to the kinetochore requires BubR1 but not Mad2 in Drosophila melanogaster.

Reduced BubR1 or Polo function results in abnormal segregation of acentric chromatids, a decrease in acentric chromosome tethering, and a great reduction in adult survival.
Title: BubR1- and Polo-coated DNA tethers facilitate poleward segregation of acentric chromatids.

BubR1's role in mitosis is discussed.
Title: Separating the spindle, checkpoint, and timer functions of BubR1.

The following BubR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BubR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12411	NM_058034.4 Latest version!	Drosophila melanogaster Bub1-related kinase (BubR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BubR1 ( NM_058034.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477382.1 Drosophila melanogaster Bub1-related kinase (BubR1), mRNA.

Price & Availability↑

CloneID	OFa12411
Clone ID Related Accession (Same CDS sequence)	NM_058034.4
Accession Version	NM_058034.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Bub1-related kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_058034.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTTTG ACAATGCGAA AGAGAACATT CAGCCGCTGG CCAGCGGTCG CAATGTGAGC 
CTTCTGCAGG CTTCTCTTAG CCAGGACTCC ACCCACGGCC AGGAACTGCT GGCACAACGC 
AAGCAAATGG AGGAGGAGGT GCGCACCTAC AAAGGCGCGG ATCCTCTCGG TGCCTGGTAC 
ACGTTCATCT GCTGGATCGA GCAGAGCTAT CCGGCGGGGG GCAGTGGCTC CGGTCTGCAG 
ACTGTCCTAC ACCAGTGCCT CACCAAATTT GAAGACGACG AGCGCTACCG TCAGGACAAG 
CGGCTTATAA AGCTTTTCAT TAAATTCATG GAGAAACAGA AGGACAAGAT CGAGTTTTAT 
CAGCAGATGT ACAACAACGG GATCGGAACA ATGCTGGCAG ATTTCTACAT TGCTTGGGCC 
TACAGCTACG ACCTGAGCGG TAACATGCGC AAGGCCGACG AGATCTTTCG CTTAGGTCTA 
GAGTGCCGCG CAGAGCCCTT AGAGGATCTG AAGGAGGCCC ACCACCACTT TGGCTATACG 
GTGGGCCAGC GCATGCTCTA CAGCACTGGT GAAGAGGCCA ACGCAGTAAA CCAGGAACTT 
AACGAGCGAC GGCTAGCACT GCAATCACTT CATGGTCGCC GGCAGCAGAT CTCTAACAGC 
ATCACCGTGG GCAGCATCCG TACCGGAGCA GCAGTTAAAA GCGGCCTCCC AGGCGTGGTT 
CAGGTCGAGC CTTCAACCAC CACCAGTAGG CGCAATGTAG GTCGGAACGT TGAGGTGTTT 
AATGACGAAA ATTCTGAGGG AAATATTCCG ATCTCTAATT CTGAGACGGA GGTGAAGTCA 
AGCCTGCGAT CCATTATCGA CTCGGCTTGC AGTCAGGAGA ATATAAAAGA ACCGGTGGCC 
TGGAATAAGG CAAATGCTAA ACGCCACAAA CATGGAAAAA TTTTCGGCAG TAACGCCTCG 
CCGGATCTGG GCTTTGATAT ACACGTTGAC GAACAAGCAA TGCCACCTAT TACAAACTAC 
GAGCGTCGTC TAGAACAACC ATTTAGATTT CCGACAAACT TTGTGGCCAA GAACAGGCCT 
CAGGAACCGT GGGTGACGCC AGTTACTATT GAGGACGAGC CAAACGCAAG TGGACTGCCT 
TGCTATAACA AGTGTCTCCT CTACCCCCGC CCCAACATCG AGTTCTCGCC CGAAGAGTAT 
CGAGCCTATT CCTTTTTGAA GCATCGCAAC CCACAGCATC CATTTATCCA TCGCAATGAT 
GATTGGTGGG GCACTGGACG GGTGATCAGA GGAATTCGCT GCTATCCGAA TTTCGCCAGA 
TATTCTAAGC CACAAGATCT CGATGAGCTA GACAAGTTTT GGAAACCCCC ACTGGTACCG 
GGCCTTCAGG TAGTTTTTGA CAAAATTTAC AACGAAGTTG AGCAAAAGGA GTACCAGCCA 
GAGGAGTTGT TGGCAGCTAA ATGGATGGAA AAACGAAATG TGACAGTTCA TGGTGACTTT 
GACATGGAAG AAACCGTTTG CTTGCCCGGA AACAAGATGC CACGCCGCAA GAGCTTCTTT 
CCCTCTTCCT TCAGAAAATC CATCATGCCA CGCAGAGTTT CGTCTGTGCA GGAGGAGGAT 
GAAAAGAAAG AGGATGAAGC CACTTTGATT GTAAAAGATA TACCTTCTCA GGTGGCACCT 
AAGATACCTG AATCGCCGTG CGATCAAATA GATGAGCCAA AAATTAAAAT ATTTGAGGAT 
TCCGGTTCTC CACGTGACAA TTTTGCTGTA CCGGCCGTTC CTGTTCCGAA AATAGAGATC 
TATGAAGACT CTGAAGAGCC GCAGCCAACT CCAAAAACAA GGCCGTTTTA TGACGGAGAT 
GAGACCTGCT CCACGCAAAT GTTCAACATG TTCATAAAGT CGCAGGCAGT AAGCACTCCT 
AAGGGAACAC AGAAACAGGC TCCGTCACGT CAATTTGGAA CCGTGCTCAA AGAACTACCG 
CTACCAGAGG AGGCTGCTCA TTCCGTCGAA TCTCCAGTGG AGACTCGTTC TCCAACTTTG 
CGAAAGCAAC TATCTACCAT ATTGGAAACC TCTGAGCACG GCACACAAAG CTTGGCGACG 
AGCGGTACCA CCACTAAATC CACTATTACC TCCACGTCTT CACCCGGATC CAATGCTGTT 
TCTAAACTAG GCAGCAAGAT TGAAGAGAAC ACTCCTGGAC AAATGCGTCT GCAGCGCGTG 
ATAAGTGCTG GTGTATCAGC AGTCCTTTCC GAGCCGGAAA AACCGGGTGG TGACAACTTT 
TTGCGCCGTG AGTTATGGGA GCCCAATGCG CCTAGCGTGC CTATGCTTAA GTCGTTGCGC 
TTCCAAGAGG ATAAGACAGA GACTATCCCA AGACCTCTTG CTTGCTTTCA GGAAGATAAA 
ACTGAAACAC TCCCACAAAT GCCGATCGCA CTGCCGGAGA ATTCTATGGA TCATGGCTCA 
CTGGGAGCTG AAAATTGTTT TTCCGCACCG CAACTTCCCA CTTTGGAGGA TGAAAGCGAT 
TTGTGTGGAT TGTTTGGAAA GACACCGCCG AAAACAAATA TTTTTGGCTC TCCGAAGCGC 
AATTTCGATC CAACTTCACA GCTTTTTAAA TCGACACAGG GCGATCGGAG ATCCTTCGCA 
GTGACTGGCA ACAAACTGGA GGCTGCGCCC AGCATCAGCA ATCTGCAGGA TTCCTTTATG 
TGCGACCTTT CCTTTGTGCC CGAAACGCAG CCTGAAGCTG TAGCAAAATC AGGGGCGATA 
CAGAAGTTTG ATATTTTTCT GGACGACACG CAGCCAAAGC TCACTAAGTC AAAACCAGTA 
GCTTTAATGG TCTCCAGCTT TGACTTAGAC TGCACGCTGC CCGAAACGCA ACAGGTGGCA 
ATTAAGGAGC CAGGGACTCA ACAGGAAAAT ATTGGGCACT CCCATATGAT TGCCTCGTTT 
ATGAAAGATT GCACAGAAAT AGGCAGCTGT CCTTCACAGA TGCCGGCTTC CGTTGTCAAA 
ACGAATATTT CAAGCACATC GAATAAGATC AAATTTTCCA ACGATTCCAT GTCAGAATCG 
TCAAGGAAAG CCCCTGTGAA TGAAAATGTC GGTATTTCAA AACAACCTGA CGAGTTTTTC 
GAGCTTAACG CAGCTACTGA AATGTTTGCA ACAAATATCA GTATGATAAA AAATTCCACA 
CTGCTGATCC CTAACGCAAA GGTAGCAGAG GCGTCGGAAC TAAGCATCTA CTATAAGACA 
ACTCCACTGA CGCCGAAGCA ATCGCACCGT TCCTGGTCGC AGTCTGATTT AGAAACTCCT 
CCGCGGGAAA ACTTTGTTCA CCCCACCTCA AACGGAGATC AAACTGTTTT GAACGAAACG 
CTGGCAGACG CTAATAAAAA CCCTTTCAAT GTAGAACTGA TCAGCTCGCT ACTCGAATCA 
ATTGACTTTT CTATGTACAT CGAAAAGCTG CCCCATTGTC AACTGGTTGG ACACGTTAAG 
CGCCTACATC CTAACACCCA CCTGGAAGTG CACAACGAAA AGTTTGAGGT GTCTAAAATG 
ATAGGAAAGG GGGCTTATGG CTCGGTTTAT GTAGGAAAGC ATCTGAAATC TGGCAAGAAG 
GTCGCGCTCA AGCAGGAACG GCCCACTAAC TACTGGGAGT TCTACATTTG TCTAGAGATT 
CACAGCCGGC TAACTAGTGA GCAAATGATT CCATCCTATG CACATATTGA CTATGCACTG 
GTAGGAAATA ATTCCAGTGT ATACATCTCA GAGTTTTCAG ACTATGGTTC TCTGATCGGT 
GTATGCAACA AGGTTAAGAG TGTCACTAAC AGGAATATGG ACGAGTATGT GGTAATGCAT 
CTGAGTTGCC AAATGCTCGA TATTGTGGAT CACCTGCACG CCATGGGTAT AATCCACGCC 
GACATCAAGC CGGATAACTT TCTACTTATG AAACCGATAT GTGCGGATCC CAACGAAGTC 
AGCCTGCAGC TTATTGATTT CGGTGTGTCT ATTGACATGA AGCTGTTTCC GGACAATCAG 
ACATTCAACT ATGTTCATCA TGATGACCTA TTCAAGTGCA TTGAGATGAG AACGGGACGC 
CCATGGACTT ACCAATTGGA TTTATATGGT CTCGTAAGCG TTATGCATGT GCTGCTTTTC 
GGCCGCTATA TGGAAGTAGT GCAGCGGTCT CCGAGCACGA TTTGGATGCC GAAAACAAAC 
GTGCCACGCT ACTTTCAGCG CACAATGTGG GAAAACATCT TCCGCACTCT GCTTAACATT 
AGAGATTGTC GCACCATGCC TAATCTGCAG CAACTGCGTA CGCAGCTTAA ATGTGCGTTG 
GCCGAAAAGG AAAAATATGT TGCCGAGGCA ATCAGCAAGT TTAACACGAT ATTGCAGAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477382.1
CDS	103..4485
Translation	MDFDNAKENIQPLASGRNVSLLQASLSQDSTHGQELLAQRKQMEEEVRTYKGADPLGAWYTFICWIEQSYPAGGSGSGLQTVLHQCLTKFEDDERYRQDKRLIKLFIKFMEKQKDKIEFYQQMYNNGIGTMLADFYIAWAYSYDLSGNMRKADEIFRLGLECRAEPLEDLKEAHHHFGYTVGQRMLYSTGEEANAVNQELNERRLALQSLHGRRQQISNSITVGSIRTGAAVKSGLPGVVQVEPSTTTSRRNVGRNVEVFNDENSEGNIPISNSETEVKSSLRSIIDSACSQENIKEPVAWNKANAKRHKHGKIFGSNASPDLGFDIHVDEQAMPPITNYERRLEQPFRFPTNFVAKNRPQEPWVTPVTIEDEPNASGLPCYNKCLLYPRPNIEFSPEEYRAYSFLKHRNPQHPFIHRNDDWWGTGRVIRGIRCYPNFARYSKPQDLDELDKFWKPPLVPGLQVVFDKIYNEVEQKEYQPEELLAAKWMEKRNVTVHGDFDMEETVCLPGNKMPRRKSFFPSSFRKSIMPRRVSSVQEEDEKKEDEATLIVKDIPSQVAPKIPESPCDQIDEPKIKIFEDSGSPRDNFAVPAVPVPKIEIYEDSEEPQPTPKTRPFYDGDETCSTQMFNMFIKSQAVSTPKGTQKQAPSRQFGTVLKELPLPEEAAHSVESPVETRSPTLRKQLSTILETSEHGTQSLATSGTTTKSTITSTSSPGSNAVSKLGSKIEENTPGQMRLQRVISAGVSAVLSEPEKPGGDNFLRRELWEPNAPSVPMLKSLRFQEDKTETIPRPLACFQEDKTETLPQMPIALPENSMDHGSLGAENCFSAPQLPTLEDESDLCGLFGKTPPKTNIFGSPKRNFDPTSQLFKSTQGDRRSFAVTGNKLEAAPSISNLQDSFMCDLSFVPETQPEAVAKSGAIQKFDIFLDDTQPKLTKSKPVALMVSSFDLDCTLPETQQVAIKEPGTQQENIGHSHMIASFMKDCTEIGSCPSQMPASVVKTNISSTSNKIKFSNDSMSESSRKAPVNENVGISKQPDEFFELNAATEMFATNISMIKNSTLLIPNAKVAEASELSIYYKTTPLTPKQSHRSWSQSDLETPPRENFVHPTSNGDQTVLNETLADANKNPFNVELISSLLESIDFSMYIEKLPHCQLVGHVKRLHPNTHLEVHNEKFEVSKMIGKGAYGSVYVGKHLKSGKKVALKQERPTNYWEFYICLEIHSRLTSEQMIPSYAHIDYALVGNNSSVYISEFSDYGSLIGVCNKVKSVTNRNMDEYVVMHLSCQMLDIVDHLHAMGIIHADIKPDNFLLMKPICADPNEVSLQLIDFGVSIDMKLFPDNQTFNYVHHDDLFKCIEMRTGRPWTYQLDLYGLVSVMHVLLFGRYMEVVQRSPSTIWMPKTNVPRYFQRTMWENIFRTLLNIRDCRTMPNLQQLRTQLKCALAEKEKYVAEAISKFNTILQK

Target ORF information:

RefSeq Version	NM_058034.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Bub1-related kinase (BubR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058034.4

ORF Insert Sequence:

ATGGACTTTG ACAATGCGAA AGAGAACATT CAGCCGCTGG CCAGCGGTCG CAATGTGAGC 
CTTCTGCAGG CTTCTCTTAG CCAGGACTCC ACCCACGGCC AGGAACTGCT GGCACAACGC 
AAGCAAATGG AGGAGGAGGT GCGCACCTAC AAAGGCGCGG ATCCTCTCGG TGCCTGGTAC 
ACGTTCATCT GCTGGATCGA GCAGAGCTAT CCGGCGGGGG GCAGTGGCTC CGGTCTGCAG 
ACTGTCCTAC ACCAGTGCCT CACCAAATTT GAAGACGACG AGCGCTACCG TCAGGACAAG 
CGGCTTATAA AGCTTTTCAT TAAATTCATG GAGAAACAGA AGGACAAGAT CGAGTTTTAT 
CAGCAGATGT ACAACAACGG GATCGGAACA ATGCTGGCAG ATTTCTACAT TGCTTGGGCC 
TACAGCTACG ACCTGAGCGG TAACATGCGC AAGGCCGACG AGATCTTTCG CTTAGGTCTA 
GAGTGCCGCG CAGAGCCCTT AGAGGATCTG AAGGAGGCCC ACCACCACTT TGGCTATACG 
GTGGGCCAGC GCATGCTCTA CAGCACTGGT GAAGAGGCCA ACGCAGTAAA CCAGGAACTT 
AACGAGCGAC GGCTAGCACT GCAATCACTT CATGGTCGCC GGCAGCAGAT CTCTAACAGC 
ATCACCGTGG GCAGCATCCG TACCGGAGCA GCAGTTAAAA GCGGCCTCCC AGGCGTGGTT 
CAGGTCGAGC CTTCAACCAC CACCAGTAGG CGCAATGTAG GTCGGAACGT TGAGGTGTTT 
AATGACGAAA ATTCTGAGGG AAATATTCCG ATCTCTAATT CTGAGACGGA GGTGAAGTCA 
AGCCTGCGAT CCATTATCGA CTCGGCTTGC AGTCAGGAGA ATATAAAAGA ACCGGTGGCC 
TGGAATAAGG CAAATGCTAA ACGCCACAAA CATGGAAAAA TTTTCGGCAG TAACGCCTCG 
CCGGATCTGG GCTTTGATAT ACACGTTGAC GAACAAGCAA TGCCACCTAT TACAAACTAC 
GAGCGTCGTC TAGAACAACC ATTTAGATTT CCGACAAACT TTGTGGCCAA GAACAGGCCT 
CAGGAACCGT GGGTGACGCC AGTTACTATT GAGGACGAGC CAAACGCAAG TGGACTGCCT 
TGCTATAACA AGTGTCTCCT CTACCCCCGC CCCAACATCG AGTTCTCGCC CGAAGAGTAT 
CGAGCCTATT CCTTTTTGAA GCATCGCAAC CCACAGCATC CATTTATCCA TCGCAATGAT 
GATTGGTGGG GCACTGGACG GGTGATCAGA GGAATTCGCT GCTATCCGAA TTTCGCCAGA 
TATTCTAAGC CACAAGATCT CGATGAGCTA GACAAGTTTT GGAAACCCCC ACTGGTACCG 
GGCCTTCAGG TAGTTTTTGA CAAAATTTAC AACGAAGTTG AGCAAAAGGA GTACCAGCCA 
GAGGAGTTGT TGGCAGCTAA ATGGATGGAA AAACGAAATG TGACAGTTCA TGGTGACTTT 
GACATGGAAG AAACCGTTTG CTTGCCCGGA AACAAGATGC CACGCCGCAA GAGCTTCTTT 
CCCTCTTCCT TCAGAAAATC CATCATGCCA CGCAGAGTTT CGTCTGTGCA GGAGGAGGAT 
GAAAAGAAAG AGGATGAAGC CACTTTGATT GTAAAAGATA TACCTTCTCA GGTGGCACCT 
AAGATACCTG AATCGCCGTG CGATCAAATA GATGAGCCAA AAATTAAAAT ATTTGAGGAT 
TCCGGTTCTC CACGTGACAA TTTTGCTGTA CCGGCCGTTC CTGTTCCGAA AATAGAGATC 
TATGAAGACT CTGAAGAGCC GCAGCCAACT CCAAAAACAA GGCCGTTTTA TGACGGAGAT 
GAGACCTGCT CCACGCAAAT GTTCAACATG TTCATAAAGT CGCAGGCAGT AAGCACTCCT 
AAGGGAACAC AGAAACAGGC TCCGTCACGT CAATTTGGAA CCGTGCTCAA AGAACTACCG 
CTACCAGAGG AGGCTGCTCA TTCCGTCGAA TCTCCAGTGG AGACTCGTTC TCCAACTTTG 
CGAAAGCAAC TATCTACCAT ATTGGAAACC TCTGAGCACG GCACACAAAG CTTGGCGACG 
AGCGGTACCA CCACTAAATC CACTATTACC TCCACGTCTT CACCCGGATC CAATGCTGTT 
TCTAAACTAG GCAGCAAGAT TGAAGAGAAC ACTCCTGGAC AAATGCGTCT GCAGCGCGTG 
ATAAGTGCTG GTGTATCAGC AGTCCTTTCC GAGCCGGAAA AACCGGGTGG TGACAACTTT 
TTGCGCCGTG AGTTATGGGA GCCCAATGCG CCTAGCGTGC CTATGCTTAA GTCGTTGCGC 
TTCCAAGAGG ATAAGACAGA GACTATCCCA AGACCTCTTG CTTGCTTTCA GGAAGATAAA 
ACTGAAACAC TCCCACAAAT GCCGATCGCA CTGCCGGAGA ATTCTATGGA TCATGGCTCA 
CTGGGAGCTG AAAATTGTTT TTCCGCACCG CAACTTCCCA CTTTGGAGGA TGAAAGCGAT 
TTGTGTGGAT TGTTTGGAAA GACACCGCCG AAAACAAATA TTTTTGGCTC TCCGAAGCGC 
AATTTCGATC CAACTTCACA GCTTTTTAAA TCGACACAGG GCGATCGGAG ATCCTTCGCA 
GTGACTGGCA ACAAACTGGA GGCTGCGCCC AGCATCAGCA ATCTGCAGGA TTCCTTTATG 
TGCGACCTTT CCTTTGTGCC CGAAACGCAG CCTGAAGCTG TAGCAAAATC AGGGGCGATA 
CAGAAGTTTG ATATTTTTCT GGACGACACG CAGCCAAAGC TCACTAAGTC AAAACCAGTA 
GCTTTAATGG TCTCCAGCTT TGACTTAGAC TGCACGCTGC CCGAAACGCA ACAGGTGGCA 
ATTAAGGAGC CAGGGACTCA ACAGGAAAAT ATTGGGCACT CCCATATGAT TGCCTCGTTT 
ATGAAAGATT GCACAGAAAT AGGCAGCTGT CCTTCACAGA TGCCGGCTTC CGTTGTCAAA 
ACGAATATTT CAAGCACATC GAATAAGATC AAATTTTCCA ACGATTCCAT GTCAGAATCG 
TCAAGGAAAG CCCCTGTGAA TGAAAATGTC GGTATTTCAA AACAACCTGA CGAGTTTTTC 
GAGCTTAACG CAGCTACTGA AATGTTTGCA ACAAATATCA GTATGATAAA AAATTCCACA 
CTGCTGATCC CTAACGCAAA GGTAGCAGAG GCGTCGGAAC TAAGCATCTA CTATAAGACA 
ACTCCACTGA CGCCGAAGCA ATCGCACCGT TCCTGGTCGC AGTCTGATTT AGAAACTCCT 
CCGCGGGAAA ACTTTGTTCA CCCCACCTCA AACGGAGATC AAACTGTTTT GAACGAAACG 
CTGGCAGACG CTAATAAAAA CCCTTTCAAT GTAGAACTGA TCAGCTCGCT ACTCGAATCA 
ATTGACTTTT CTATGTACAT CGAAAAGCTG CCCCATTGTC AACTGGTTGG ACACGTTAAG 
CGCCTACATC CTAACACCCA CCTGGAAGTG CACAACGAAA AGTTTGAGGT GTCTAAAATG 
ATAGGAAAGG GGGCTTATGG CTCGGTTTAT GTAGGAAAGC ATCTGAAATC TGGCAAGAAG 
GTCGCGCTCA AGCAGGAACG GCCCACTAAC TACTGGGAGT TCTACATTTG TCTAGAGATT 
CACAGCCGGC TAACTAGTGA GCAAATGATT CCATCCTATG CACATATTGA CTATGCACTG 
GTAGGAAATA ATTCCAGTGT ATACATCTCA GAGTTTTCAG ACTATGGTTC TCTGATCGGT 
GTATGCAACA AGGTTAAGAG TGTCACTAAC AGGAATATGG ACGAGTATGT GGTAATGCAT 
CTGAGTTGCC AAATGCTCGA TATTGTGGAT CACCTGCACG CCATGGGTAT AATCCACGCC 
GACATCAAGC CGGATAACTT TCTACTTATG AAACCGATAT GTGCGGATCC CAACGAAGTC 
AGCCTGCAGC TTATTGATTT CGGTGTGTCT ATTGACATGA AGCTGTTTCC GGACAATCAG 
ACATTCAACT ATGTTCATCA TGATGACCTA TTCAAGTGCA TTGAGATGAG AACGGGACGC 
CCATGGACTT ACCAATTGGA TTTATATGGT CTCGTAAGCG TTATGCATGT GCTGCTTTTC 
GGCCGCTATA TGGAAGTAGT GCAGCGGTCT CCGAGCACGA TTTGGATGCC GAAAACAAAC 
GTGCCACGCT ACTTTCAGCG CACAATGTGG GAAAACATCT TCCGCACTCT GCTTAACATT 
AGAGATTGTC GCACCATGCC TAATCTGCAG CAACTGCGTA CGCAGCTTAA ATGTGCGTTG 
GCCGAAAAGG AAAAATATGT TGCCGAGGCA ATCAGCAAGT TTAACACGAT ATTGCAGAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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BubR1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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