LeuRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LeuRS-m
Entrez Gene ID	38581
Full Name	Leucyl-tRNA synthetase, mitochondrial
Synonyms	Aats-leu,CG7479,Dmel\CG7479,LRS,LeuRS
General protein information	Preferred Names Leucyl-tRNA synthetase, mitochondrial Names CG7479 gene product from transcript CG7479-RA CG7479-PA LeuRS-m-PA Leucyl-tRNA synthetase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64C1-64C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_139675.3	NP_647932.1	Leucyl-tRNA synthetase, mitochondrial

Pathways from BioSystems

Homologies

	LARS2	XP_001114552.2
Homo sapiens (human)	LARS2	NP_056155.1
Kluyveromyces lactis	KLLA0E05325g	XP_454185.1
Magnaporthe oryzae (rice blast fungus)	MGG_04042	XP_003719800.1
Arabidopsis thaliana (thale cress)	EMB2369	NP_192344.1
Canis lupus familiaris (dog)	LARS2	XP_005632695.1
Rattus norvegicus (Norway rat)	Lars2	NP_001102257.1
Danio rerio (zebrafish)	lars2	NP_001099171.1
Drosophila melanogaster (fruit fly)	Aats-leu	NP_647932.1
Schizosaccharomyces pombe (fission yeast)	SPAC4G8.09	NP_593069.1
Neurospora crassa	NCU03814	XP_961203.1
Pan troglodytes (chimpanzee)	LARS2	XP_001147749.1
Mus musculus (house mouse)	Lars2	NP_694808.1
Caenorhabditis elegans (roundworm)	lars-2	NP_001021875.1
Saccharomyces cerevisiae (baker's yeast)	NAM2	NP_013486.3
Gallus gallus (chicken)	LARS2	XP_004939517.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002014	XP_321043.4
Eremothecium gossypii	AGOS_AER043C	NP_984903.1
Xenopus tropicalis (tropical clawed frog)	lars2	XP_002938354.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LeuRS-m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LeuRS-m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17553	NM_139675.3 Latest version!	Drosophila melanogaster Leucyl-tRNA synthetase, mitochondrial (LeuRS-m), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LeuRS-m ( NM_139675.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647932.1 Drosophila melanogaster Leucyl-tRNA synthetase, mitochondrial (LeuRS-m), mRNA.

Price & Availability↑

CloneID	OFa17553
Clone ID Related Accession (Same CDS sequence)	NM_139675.3
Accession Version	NM_139675.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Leucyl-tRNA synthetase, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139675.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAGCAC TGAGAAGAAC ACGACCTTGG GGAGCTGTCT ACTGGAATAG CTGGCGGCGC 
CTGCTAACCC AAAGCTGCAC CCGGAATGAT AATCCAGAGC TAACCAGTGA TGTCAAGCAC 
CGGATTGAGG CTCATTGGCG GGAGCAGCTC AGTGGAGGAC AGTTCAATCC CAAGGACTCG 
CAGGACAAGT ACTACGTGCT CTCCATGTTC CCGTATCCCT CCGGCAATCT TCACATGGGC 
CATGTGCGCG TCTATACCAT AGCCGATTCG GTGGCTCGTT TCCAGCGAAT GTGCGGCAAG 
AATGTGTTTC AGCCAATGGG TTGGGATTCC TTTGGGCTGC CCGCCGAGAA CGCCGCCAAT 
CAGCGTGGGG TGGAGCCCGC GTCCTGGACG GAGCAGAACA TTGCCCAGAT GAAGGAACAA 
CTCAAGCGAC TTGGTTGCTC CTTTGACTGG AACCATGAAT TGTCCACCTG CAGTCCAAAA 
TACTACAAGT GGACGCAGCA TCTGTTCCTA ATGCTCCATC GCCATGGATT GGCCTACCAG 
AACGAGGCTC TTGTTAACTG GGATCCCGTT GATAAGACCG TGCTCGCAGA TGAGCAGGTG 
GACGCCAACG GCTGTTCCTG GCGGTCGGGC GCCAAGGTGG AAAAGAAGCT TTTGAGGCAG 
TGGTTTATCC GAACGAGTGC TTATGCCAAA CAATTGCTAG ACGGTTTGGA GGATCCCACG 
CTTCGCGACT GGAGGGATAT TATTAACCTG CAGCGACACT GGATTGGAGA GTGTGATGGA 
TACGCTTTCA ACCTGCTCAC CTCCGCCTCC GGACTTTTAC GAGTGTGGAC TACCCATCCG 
GAGCATTTAA AAGATCCCCA TGCCTTTCTC GTGCTCCGCA GCAATCATCA CTTGTCAAAG 
CTAAAGGGTG CGGACAGCCT AAGCGCAGCG AACCCGTTTG CGGGCACCAC AATGCCTGTG 
GTCTTTAGCG ACGAAGTGAC ATTTCCGCCA AAGAGCGATG TTTACCTGGC AGCCCCCAGC 
TTCCGTGGCG AAGACAAGGA TTTGTGGGAT TCATATGGAC TGGCTTTTAC TTCAAATGGC 
AAGGATGAGG AATCCCTAAG TCCAGCCAAC TGGGAAATGT TAAGGAAAGA GGTGCTCCAG 
GCAGCCACAC GCCTTAATGT TGGCGGCTAT CGTGTATCCT CAAAGCTGAA GGATTGGTTG 
ATATCACGAC AGCGCTATTG GGGCACTCCC ATTCCTATTG TACACTGTCA AAAGTGCGGA 
GCAGTGCCAG TGCCGGAGGA GCAGCTGCCA GTGTCTCTGC CTCCGAAGGA TTCCCCGAAG 
GAAGCGTTTC TCTGTGATTG CCCAAAATGC GGTGAGAAGG ATGCACGAAG GGAGACGGAT 
ACAATGGATA CATTTGTGGA CAGCTCTTGG TACTACTTGC GTTACTTGGA CGCCCAAAAT 
TCCGAACGCA TTTTCGATAC AGCGCTGACC AAGAAGTTTA TGCCTGTGGA TCTGTACATA 
GGAGGCAAGG AACATGCCGT GCTCCATCTT TACTACGCCC GCTTTATAAA CCACTTCCTG 
CACAGTTGCG GGCTTTCGCC CACTAGCGAA CCCTTTTCCC GGCTGCTAGT CCAGGGCATG 
GTGATGGGTC GCTCCTTCCG GGTGAAGGGC AGTGGACGTT ATGTGCCCGA AACAGAAGTG 
GAGATTGTGA ACGCCAAAAA GAACCAGGCG GTGCTAAAGG AAACCAAAGA GCCCGTGGTC 
ATGACCTGGG AAAAGATGTC TAAGTCAAAG CTGAATGGGG TGGAGCCTAG CGACATGTTT 
AATGAGTATG GAACGGATAC AACGAGACTG ATCATATTGG CCGATGTGGC GCCCACATCC 
CACCGCAACT GGTCCAGTGC AACTTTTCCT GGCATCCTTA ATTGGCAAAA GCGGCTTTGG 
CTGACCCTGC AGGACTTCCA ACAGGCTCGC GAAGATCAAA CTGCTTCGGA TGTTGTACCC 
ACCAGTGAAG AGTTCCTGGC TGAGGATGCC AAGCTGTTCG ATGCGCGAAA CTTTTATGTG 
AAAGGAGCCA CCTTTAACTA CCGCCATGCA CAGCAACTCA GTGTGGCAAT TTCCAAAATG 
CAGGGGCTAA CGAATTCTCT CAGGCGCACA CCTAAGCATG TCCTGCGACA TGGAAAACAG 
TTTGAACGTG CGCTGGCTGC CCAGATCATC ATGCTCGCGC CAATGGCTCC GCACTTTGCC 
TCCGAACTGT GGTCAAAGTT TGTGGCGATA CCAGGCAGGC TAAATCCCGC CAGTCAGGAG 
TTACAGTGGA GCGAAGATGT GCTTGCTCAG CGCTGGCCAG ACATCGACCC CGCCTACAAT 
CTGGACCTTA GCATTAAAGT CAATGGATTT GAGAACTGTG TAATCAAAGT GCAGAGAACG 
CATCTGGATA AGGTAACGCA CAGTGATGCT CTCGACATTG CCTTCAACAC CGAATCGGTG 
ACATCGTACC TAATTGACAA GAAAATTCGC ACCACCAATT TTGTGCTTTA CCCGGGAATC 
GAGGCTATTC TGAATATCTA TGTGGATAAG GCGAAAATGA AGCAAAAGCC GCCGACCACC 
GATGATGCAG AAGCTCAGCC TCAGGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647932.1
CDS	101..2710
Translation	MQALRRTRPWGAVYWNSWRRLLTQSCTRNDNPELTSDVKHRIEAHWREQLSGGQFNPKDSQDKYYVLSMFPYPSGNLHMGHVRVYTIADSVARFQRMCGKNVFQPMGWDSFGLPAENAANQRGVEPASWTEQNIAQMKEQLKRLGCSFDWNHELSTCSPKYYKWTQHLFLMLHRHGLAYQNEALVNWDPVDKTVLADEQVDANGCSWRSGAKVEKKLLRQWFIRTSAYAKQLLDGLEDPTLRDWRDIINLQRHWIGECDGYAFNLLTSASGLLRVWTTHPEHLKDPHAFLVLRSNHHLSKLKGADSLSAANPFAGTTMPVVFSDEVTFPPKSDVYLAAPSFRGEDKDLWDSYGLAFTSNGKDEESLSPANWEMLRKEVLQAATRLNVGGYRVSSKLKDWLISRQRYWGTPIPIVHCQKCGAVPVPEEQLPVSLPPKDSPKEAFLCDCPKCGEKDARRETDTMDTFVDSSWYYLRYLDAQNSERIFDTALTKKFMPVDLYIGGKEHAVLHLYYARFINHFLHSCGLSPTSEPFSRLLVQGMVMGRSFRVKGSGRYVPETEVEIVNAKKNQAVLKETKEPVVMTWEKMSKSKLNGVEPSDMFNEYGTDTTRLIILADVAPTSHRNWSSATFPGILNWQKRLWLTLQDFQQAREDQTASDVVPTSEEFLAEDAKLFDARNFYVKGATFNYRHAQQLSVAISKMQGLTNSLRRTPKHVLRHGKQFERALAAQIIMLAPMAPHFASELWSKFVAIPGRLNPASQELQWSEDVLAQRWPDIDPAYNLDLSIKVNGFENCVIKVQRTHLDKVTHSDALDIAFNTESVTSYLIDKKIRTTNFVLYPGIEAILNIYVDKAKMKQKPPTTDDAEAQPQA

Target ORF information:

RefSeq Version	NM_139675.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Leucyl-tRNA synthetase, mitochondrial (LeuRS-m), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139675.3

ORF Insert Sequence:

ATGCAAGCAC TGAGAAGAAC ACGACCTTGG GGAGCTGTCT ACTGGAATAG CTGGCGGCGC 
CTGCTAACCC AAAGCTGCAC CCGGAATGAT AATCCAGAGC TAACCAGTGA TGTCAAGCAC 
CGGATTGAGG CTCATTGGCG GGAGCAGCTC AGTGGAGGAC AGTTCAATCC CAAGGACTCG 
CAGGACAAGT ACTACGTGCT CTCCATGTTC CCGTATCCCT CCGGCAATCT TCACATGGGC 
CATGTGCGCG TCTATACCAT AGCCGATTCG GTGGCTCGTT TCCAGCGAAT GTGCGGCAAG 
AATGTGTTTC AGCCAATGGG TTGGGATTCC TTTGGGCTGC CCGCCGAGAA CGCCGCCAAT 
CAGCGTGGGG TGGAGCCCGC GTCCTGGACG GAGCAGAACA TTGCCCAGAT GAAGGAACAA 
CTCAAGCGAC TTGGTTGCTC CTTTGACTGG AACCATGAAT TGTCCACCTG CAGTCCAAAA 
TACTACAAGT GGACGCAGCA TCTGTTCCTA ATGCTCCATC GCCATGGATT GGCCTACCAG 
AACGAGGCTC TTGTTAACTG GGATCCCGTT GATAAGACCG TGCTCGCAGA TGAGCAGGTG 
GACGCCAACG GCTGTTCCTG GCGGTCGGGC GCCAAGGTGG AAAAGAAGCT TTTGAGGCAG 
TGGTTTATCC GAACGAGTGC TTATGCCAAA CAATTGCTAG ACGGTTTGGA GGATCCCACG 
CTTCGCGACT GGAGGGATAT TATTAACCTG CAGCGACACT GGATTGGAGA GTGTGATGGA 
TACGCTTTCA ACCTGCTCAC CTCCGCCTCC GGACTTTTAC GAGTGTGGAC TACCCATCCG 
GAGCATTTAA AAGATCCCCA TGCCTTTCTC GTGCTCCGCA GCAATCATCA CTTGTCAAAG 
CTAAAGGGTG CGGACAGCCT AAGCGCAGCG AACCCGTTTG CGGGCACCAC AATGCCTGTG 
GTCTTTAGCG ACGAAGTGAC ATTTCCGCCA AAGAGCGATG TTTACCTGGC AGCCCCCAGC 
TTCCGTGGCG AAGACAAGGA TTTGTGGGAT TCATATGGAC TGGCTTTTAC TTCAAATGGC 
AAGGATGAGG AATCCCTAAG TCCAGCCAAC TGGGAAATGT TAAGGAAAGA GGTGCTCCAG 
GCAGCCACAC GCCTTAATGT TGGCGGCTAT CGTGTATCCT CAAAGCTGAA GGATTGGTTG 
ATATCACGAC AGCGCTATTG GGGCACTCCC ATTCCTATTG TACACTGTCA AAAGTGCGGA 
GCAGTGCCAG TGCCGGAGGA GCAGCTGCCA GTGTCTCTGC CTCCGAAGGA TTCCCCGAAG 
GAAGCGTTTC TCTGTGATTG CCCAAAATGC GGTGAGAAGG ATGCACGAAG GGAGACGGAT 
ACAATGGATA CATTTGTGGA CAGCTCTTGG TACTACTTGC GTTACTTGGA CGCCCAAAAT 
TCCGAACGCA TTTTCGATAC AGCGCTGACC AAGAAGTTTA TGCCTGTGGA TCTGTACATA 
GGAGGCAAGG AACATGCCGT GCTCCATCTT TACTACGCCC GCTTTATAAA CCACTTCCTG 
CACAGTTGCG GGCTTTCGCC CACTAGCGAA CCCTTTTCCC GGCTGCTAGT CCAGGGCATG 
GTGATGGGTC GCTCCTTCCG GGTGAAGGGC AGTGGACGTT ATGTGCCCGA AACAGAAGTG 
GAGATTGTGA ACGCCAAAAA GAACCAGGCG GTGCTAAAGG AAACCAAAGA GCCCGTGGTC 
ATGACCTGGG AAAAGATGTC TAAGTCAAAG CTGAATGGGG TGGAGCCTAG CGACATGTTT 
AATGAGTATG GAACGGATAC AACGAGACTG ATCATATTGG CCGATGTGGC GCCCACATCC 
CACCGCAACT GGTCCAGTGC AACTTTTCCT GGCATCCTTA ATTGGCAAAA GCGGCTTTGG 
CTGACCCTGC AGGACTTCCA ACAGGCTCGC GAAGATCAAA CTGCTTCGGA TGTTGTACCC 
ACCAGTGAAG AGTTCCTGGC TGAGGATGCC AAGCTGTTCG ATGCGCGAAA CTTTTATGTG 
AAAGGAGCCA CCTTTAACTA CCGCCATGCA CAGCAACTCA GTGTGGCAAT TTCCAAAATG 
CAGGGGCTAA CGAATTCTCT CAGGCGCACA CCTAAGCATG TCCTGCGACA TGGAAAACAG 
TTTGAACGTG CGCTGGCTGC CCAGATCATC ATGCTCGCGC CAATGGCTCC GCACTTTGCC 
TCCGAACTGT GGTCAAAGTT TGTGGCGATA CCAGGCAGGC TAAATCCCGC CAGTCAGGAG 
TTACAGTGGA GCGAAGATGT GCTTGCTCAG CGCTGGCCAG ACATCGACCC CGCCTACAAT 
CTGGACCTTA GCATTAAAGT CAATGGATTT GAGAACTGTG TAATCAAAGT GCAGAGAACG 
CATCTGGATA AGGTAACGCA CAGTGATGCT CTCGACATTG CCTTCAACAC CGAATCGGTG 
ACATCGTACC TAATTGACAA GAAAATTCGC ACCACCAATT TTGTGCTTTA CCCGGGAATC 
GAGGCTATTC TGAATATCTA TGTGGATAAG GCGAAAATGA AGCAAAAGCC GCCGACCACC 
GATGATGCAG AAGCTCAGCC TCAGGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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LeuRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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