DNApol-alpha50 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNApol-alpha50
Entrez Gene ID	38942
Full Name	DNA polymerase alpha 50kD
Synonyms	CG7108,DNA polalpha,DNApol-alpha,DNApolalpha,DNApolalpha-50,DNApolalpha50,Dmel\CG7108,Dmpol alpha,Dpri50,POLA,PRI1,Pol,Polalpha,Polalpha 50kDa,anon-WO0140519.257,dnapol alpha 50,pol alpha,polalpha,polalpha50
General protein information	Preferred Names DNA polymerase alpha 50kD Names CG7108 gene product from transcript CG7108-RA CG7108-PA DNA polymerase 50K DNA polymerase alpha DNA polymerase alpha 46,000 gamma subunit DNA polymerase alpha 50 kda primase subunit DNA polymerase alpha 50,000 gamma subunit DNA-polymerase alpha DNApol-alpha50-PA p50 subunit of DNA polymerase alpha
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66C7-66C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_079248.4	NP_523972.2	DNA polymerase alpha 50kD

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRIM1	NP_000937.1
Mus musculus (house mouse)	Prim1	NP_032947.1
Caenorhabditis elegans (roundworm)	pri-1	NP_001255015.1
Eremothecium gossypii	AGOS_AAR018W	NP_982559.1
Schizosaccharomyces pombe (fission yeast)	spp1	NP_593765.1
Xenopus tropicalis (tropical clawed frog)	prim1	NP_998870.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006065	XP_316116.4
Caenorhabditis elegans (roundworm)	T24C4.5	NP_497283.2
Kluyveromyces lactis	KLLA0B11682g	XP_452050.1
Arabidopsis thaliana (thale cress)	POLA3	NP_199003.5
Pan troglodytes (chimpanzee)	PRIM1	XP_001168535.1
Canis lupus familiaris (dog)	PRIM1	XP_005625579.1
Bos taurus (cattle)	PRIM1	NP_001139337.1
Gallus gallus (chicken)	PRIM1	XP_424273.4
	LOC710253	XP_001098855.2
Danio rerio (zebrafish)	prim1	NP_958856.1
Drosophila melanogaster (fruit fly)	DNApol-alpha50	NP_523972.2
Magnaporthe oryzae (rice blast fungus)	MGG_00881	XP_003718088.1
Neurospora crassa	NCU02232	XP_959645.1
Saccharomyces cerevisiae (baker's yeast)	PRI1	NP_012273.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DNApol-alpha50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNApol-alpha50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20716	NM_079248.4 Latest version!	Drosophila melanogaster DNA polymerase alpha 50kD (DNApol-alpha50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNApol-alpha50 ( NM_079248.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523972.2 Drosophila melanogaster DNA polymerase alpha 50kD (DNApol-alpha50), mRNA.

Price & Availability↑

CloneID	OFa20716
Clone ID Related Accession (Same CDS sequence)	NM_079248.4
Accession Version	NM_079248.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	DNA polymerase alpha 50kD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079248.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGAAC AAGTCACAGA TCAGGAGAAC CAGGCTCCAC AGCAGCAGAC TACTGCTGTG 
CATGCCTACA ATCCCGAAGT CCTGCAGGAT ATGTTGCCCG TGTATTACAG GCGCCTCTTT 
CCCCACCTGC CCTTCTACCG TTGGCTATCG TATGGATCAT CCGAGGATGC TATCTTTTCC 
AATCGTGAAA TTTCCTTTAC ACTGCAGGAC GATATCTACA TACGTTACCT CTGCTTTGAT 
ACCCAAGCTG AATTGGAAAA GGAGATCTGC TCGAGGAATC CTATTAAGAT TGACATAGGA 
CCAGTAATGC ATTCCAAACC CAAAAACCAT CGTTCAATTC CTGGCGGCTT AACGCCCGTA 
CAACGTGAAC TCGTTTTTGA TATAGATATG ACGGATTACG ACGAGGTGCG TACCTGTTGC 
TCTGGAGCAG GGGTATGCCT GAAATGCTGG AAGTTTATGG TGCTGGCTGC CAGGGTTTTG 
GATGTCGCTT TGCGCGAGGA TTTCGGTTTC GAGCACATAA TCTGGATTTT CTCAGGTAGA 
CGAGGTATCC ATTGCTGGGT TTGCGACTAC CAAGCCCGTC ACTTGGACGG ACGTGGTCGC 
TATGCTGTGG CGGAATACCT TAACATTATT ACCTATGCCA GTTTCGCGGG CGGCAATTCA 
CCCAGATGTT CAATGGGAGA CCGACCGCAT CATAGCCTAA AGAGAGCCTT AAAGATAGTG 
GAGCCCATGT TCGAGGAGAT TGTACTAGAG GATCAGAATC TTTTTGGAAC TCCCAAGGGA 
GTTACCAAAC TTCTTAACAT GGTCCACGAT GACGCTGCGC GTGGCGAACT AGAATCTTAT 
ATGCAAAAGA ATTTGGAGGA TGGAGCACAC TCGCGACTCG TTTGGGAGAG CTTCATTAAG 
TACGCCAACT CTATGAGAAC ATCCACGACA AGTGCCTGGA GTCGCAAGCT GAAGAACATT 
GTAGCCGAAA TCCAGCTTGG ACTGCTCTAT CCTCGTTTGG ACATCAATGT CACTAGGGGA 
TTCAATCATT TGCTAAAGGC ACCGTTTTGC ATTCATCCAG CCACTGGAAA AGTGTGCGTT 
CCGTTTAGTG TGAGTGCTGT GGCCAAGTTT GATCCCACCA CAGTGCCGAC GATCACCCAG 
TTGCTTCACG AGATCAACGC TTTCGATGAT AAGAGCAAGA GCTACATGGA GGCGCCGGAG 
GATAAGAGCC GCATCAAGGA TCACAAGAAG ACTAGCATGT TCAAGGGTGT CGTGGTTTTC 
GAGGAGTTCC TGCGAAAACT GGAGCGCAGC CAGAAATCTG CCAGCTTGCA GTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523972.2
CDS	196..1512
Translation	MPEQVTDQENQAPQQQTTAVHAYNPEVLQDMLPVYYRRLFPHLPFYRWLSYGSSEDAIFSNREISFTLQDDIYIRYLCFDTQAELEKEICSRNPIKIDIGPVMHSKPKNHRSIPGGLTPVQRELVFDIDMTDYDEVRTCCSGAGVCLKCWKFMVLAARVLDVALREDFGFEHIIWIFSGRRGIHCWVCDYQARHLDGRGRYAVAEYLNIITYASFAGGNSPRCSMGDRPHHSLKRALKIVEPMFEEIVLEDQNLFGTPKGVTKLLNMVHDDAARGELESYMQKNLEDGAHSRLVWESFIKYANSMRTSTTSAWSRKLKNIVAEIQLGLLYPRLDINVTRGFNHLLKAPFCIHPATGKVCVPFSVSAVAKFDPTTVPTITQLLHEINAFDDKSKSYMEAPEDKSRIKDHKKTSMFKGVVVFEEFLRKLERSQKSASLQF

Target ORF information:

RefSeq Version	NM_079248.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DNA polymerase alpha 50kD (DNApol-alpha50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079248.4

ORF Insert Sequence:

ATGCCCGAAC AAGTCACAGA TCAGGAGAAC CAGGCTCCAC AGCAGCAGAC TACTGCTGTG 
CATGCCTACA ATCCCGAAGT CCTGCAGGAT ATGTTGCCCG TGTATTACAG GCGCCTCTTT 
CCCCACCTGC CCTTCTACCG TTGGCTATCG TATGGATCAT CCGAGGATGC TATCTTTTCC 
AATCGTGAAA TTTCCTTTAC ACTGCAGGAC GATATCTACA TACGTTACCT CTGCTTTGAT 
ACCCAAGCTG AATTGGAAAA GGAGATCTGC TCGAGGAATC CTATTAAGAT TGACATAGGA 
CCAGTAATGC ATTCCAAACC CAAAAACCAT CGTTCAATTC CTGGCGGCTT AACGCCCGTA 
CAACGTGAAC TCGTTTTTGA TATAGATATG ACGGATTACG ACGAGGTGCG TACCTGTTGC 
TCTGGAGCAG GGGTATGCCT GAAATGCTGG AAGTTTATGG TGCTGGCTGC CAGGGTTTTG 
GATGTCGCTT TGCGCGAGGA TTTCGGTTTC GAGCACATAA TCTGGATTTT CTCAGGTAGA 
CGAGGTATCC ATTGCTGGGT TTGCGACTAC CAAGCCCGTC ACTTGGACGG ACGTGGTCGC 
TATGCTGTGG CGGAATACCT TAACATTATT ACCTATGCCA GTTTCGCGGG CGGCAATTCA 
CCCAGATGTT CAATGGGAGA CCGACCGCAT CATAGCCTAA AGAGAGCCTT AAAGATAGTG 
GAGCCCATGT TCGAGGAGAT TGTACTAGAG GATCAGAATC TTTTTGGAAC TCCCAAGGGA 
GTTACCAAAC TTCTTAACAT GGTCCACGAT GACGCTGCGC GTGGCGAACT AGAATCTTAT 
ATGCAAAAGA ATTTGGAGGA TGGAGCACAC TCGCGACTCG TTTGGGAGAG CTTCATTAAG 
TACGCCAACT CTATGAGAAC ATCCACGACA AGTGCCTGGA GTCGCAAGCT GAAGAACATT 
GTAGCCGAAA TCCAGCTTGG ACTGCTCTAT CCTCGTTTGG ACATCAATGT CACTAGGGGA 
TTCAATCATT TGCTAAAGGC ACCGTTTTGC ATTCATCCAG CCACTGGAAA AGTGTGCGTT 
CCGTTTAGTG TGAGTGCTGT GGCCAAGTTT GATCCCACCA CAGTGCCGAC GATCACCCAG 
TTGCTTCACG AGATCAACGC TTTCGATGAT AAGAGCAAGA GCTACATGGA GGCGCCGGAG 
GATAAGAGCC GCATCAAGGA TCACAAGAAG ACTAGCATGT TCAAGGGTGT CGTGGTTTTC 
GAGGAGTTCC TGCGAAAACT GGAGCGCAGC CAGAAATCTG CCAGCTTGCA GTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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DNApol-alpha50 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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