Fhos gene cDNA ORF clone, Drosophila melanogaster(fruit fly)

mRNA	Protein	Name
NM_001259755.1	NP_001246684.1	formin homology 2 domain containing, isoform H
NM_168294.3	NP_729411.1	formin homology 2 domain containing, isoform B
NM_001259754.2	NP_001246683.1	formin homology 2 domain containing, isoform G
NM_001169916.2	NP_001163387.2	formin homology 2 domain containing, isoform J
NM_001202143.2	NP_001189072.1	formin homology 2 domain containing, isoform D
NM_001274670.1	NP_001261599.1	formin homology 2 domain containing, isoform I
NM_168293.3	NP_729410.1	formin homology 2 domain containing, isoform A
NM_001202142.2	NP_001189071.1	formin homology 2 domain containing, isoform E
NM_001259753.2	NP_001246682.1	formin homology 2 domain containing, isoform F

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17939	NM_168294.3 Latest version!	Drosophila melanogaster formin homology 2 domain containing, transcript variant B (Fhos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20761	NM_001259754.2 Latest version!	Drosophila melanogaster formin homology 2 domain containing, transcript variant G (Fhos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa20762	NM_001259753.2 Latest version!	Drosophila melanogaster formin homology 2 domain containing, transcript variant F (Fhos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa17938	NM_168293.3 Latest version!	Drosophila melanogaster formin homology 2 domain containing, transcript variant A (Fhos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa20758	NM_001259755.1 Latest version!	Drosophila melanogaster formin homology 2 domain containing, transcript variant H (Fhos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa20759	NM_001202143.2 Latest version!	Drosophila melanogaster formin homology 2 domain containing, transcript variant D (Fhos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa20760	NM_001202142.2 Latest version!	Drosophila melanogaster formin homology 2 domain containing, transcript variant E (Fhos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa20757	NM_001274670.1 Latest version!	Drosophila melanogaster formin homology 2 domain containing, transcript variant I (Fhos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa20757	NM_001169916.2 Latest version!	Drosophila melanogaster formin homology 2 domain containing, transcript variant J (Fhos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

Fhos ( NM_168294.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729411.1 Drosophila melanogaster formin homology 2 domain containing, transcript variant B (Fhos), mRNA.

Price & Availability↑

CloneID	OFa17939
Clone ID Related Accession (Same CDS sequence)	NM_168294.3
Accession Version	NM_168294.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	formin homology 2 domain containing, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168294.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGTGA AAATGGAGCC GGACGAACTG ATCACATTCC GTGTCCAGTA TCTGGTGGAC 
AGCGATCCGC TCTCGGCGTG CACCGCCTTC CCGATCCCGG TGCGGGCGCC CACCTACGCC 
TTCGCCACCA CCATGCCGCT GGCCAATCAG CTGGGCACAA TCCTGCGGCT GCTGAATGCA 
CCACAAAGCA TCGACGATGC AGCCATTCAG GTGTACAAGG ATGGCGACTA CGGAGCCTAC 
TTGGATCTGG AGTCATCGCT GGCCGAACAG GCCGAGGAAA TCGAGGGAGT CAACGTCAGC 
CGCAAAAACT CTCTGGTGGT GCGAACGCAG TTAAATGTGC GCGTACAAGC CATAATTGAG 
AAACTGCTCT CCGCACAGGG CAGTGACCTG CGGCGTGCTC TCTTCTCGCT GAAGCAGGTG 
TTCCAGGAGG ATAAGGATCT GGTGCACGCC TTCGTGGCCC TCGGCGGCCT CAATTGCCTG 
GTGCGCGTGG GCAATTGTGC GGATCAAAAC TATCAGAATT ACATTCTGCG AGCCCTCGGT 
CAGGTCATGC TCTATGTGGA CGGAATGAAC GGCGTGATGA AGCATGAGCC GACGATGCAG 
TGGCTCTACT CGCTGATTGC CTCCAATTAC CGATCCGTGG TGAAGACCGC CCTCAAGTTG 
TTGCTGGTCT TCGTGGAGTA CGCCGAGTCC AACTGCTATG TGCTGGTCAG TGCCATTCAC 
GCAGTGGATG CTTCCCAGGG CACACTGCCC TGGTCAAATA TAATGAGACT GCTTAAGGAC 
TATGACAATG CTGATGCCGA ACTGGTGATC TATGCCACTT CGCTGATTAA CAAAACTCTG 
GCGGGTCTGA ATGATCAGGA CAGCTTCTAC GACGAAAGCG ATCTTCTAGA GCAGCAGGGC 
ATGGAGACTG TGATCCAGCG TTATATGTCC AAGCCGGGCA CTGATTTGGA TCTGCTCGAT 
CAGTTGCAAC TGTATGAGGC GGTTCTCAAA TTTGAGGATG GTGAGTCGGA TGGACAGCGT 
TTGCCGCAGA ACTCAATGCG AAAAACACAA CGCTATCGTC CGGGAGCAAA TACCACTGAA 
AGACGCAAGT CGCGTCGCCA TAGCACAGAT AATAGTCCCG CATCACTCAC AAAGGTCCTG 
CCCACCACTG TCCTCCGGAT GACACCTACA CAAGCTACGG TGGATGAGGA TAGTTCTGGC 
TCCACCAACT CAACGGAATT TAGTGGCGGT CTCTTTAATG AGAAGAAACC TCGTGATGGT 
GCAGGCGTGA CACCAGGACT TCGAAGAAGA CGTGAGCGGG CGGAGCGGCA TAAGAGCTTT 
CTGAAGGAGC AACAGGAGGC GGCGGCCAAT GGAATCTTTG CTGCTCTCGA GCAGCGGGAG 
GGACTGGGCC AGATTGTAAC CGCCATACCC GCTACCATTC AAACCGGCGA TAGTCCGCCT 
CAGTCCTTGC AACATGAGGT GCCACCGTGC AACAACATCA GCGGAATCAC CAACATTAGT 
AACAACAATG TCAGCAACCT TAGCTGCAAT GACACGCCGA ACAACAGCCT CTTGGTGATG 
AGTCCTAGCA AGCAACTGCT CACTGGAAGC CACAGCATCT CCATTATTAA CAACAGCTTC 
GATAAGGCGA GCAATAACAA TCAGAGCGAG TTCCTGACCA AGAAGAACCT ATTCCGGGAA 
CGCAATTCAA CTGTGATCAA TGGTATTATG AAGAATATCC AGCAAACCGA TAGTGCCTAT 
AAGAAGTACG AGAACGAAGC GAACCGGCTG AACAACTATT ACAGTCAGTC GCCGAAACAT 
CAGATCCATC AGCCCATTCA GCCAACGCCG GAAGTCCTTT TGAGTCCCAA AAAAACGCTA 
AATGCCTCTG GGTTTGATTT CACGCCCGCT CCCCTCAGTT CCACCCCGGT TAAGTGTGAT 
GTCCCGCCGG AACCGGAGCC ACTGAGTCCG GTAAAGAAAC CCGCTGCGGC ACCACCACCG 
CCTCCTCCAC CGCCACCGCC GCCACCACCA CCACCTGGTT GGTGGCACGC CCCCATCTAC 
AACAGCCCGC AAGCTCGAAC CCTAACCAGT ACTAACCCAG AGCTGCTGCC GCATCCTGTG 
GATCCTTCAG ATATGGAAAG CGAGGATCAG CAGCTGCTCC TCCAGCTGAA GCGGGACCAC 
ACGGTCAAGG ATCTCACCCA GAAGTTGAGC AACCTGCCAA TGAGTCCCAC TCAGGATTCG 
GCGAATCGCG CCCTCGTCGG CGATATGTCC GGTCTGATTT CGAAAGCCAA AGAGGGTCTG 
GCCAAGAGCA AGAGCAAGGG AGAGATATCC AGATCTTCTA GTGTCGATCA GGATGTAAAG 
AAGTCTGAGC CCAAGAAGTC GGAAAATGAA CTTCACTGGG AGGAACTGGT TAGGAATATG 
ACAAGGCCCC TAAATCTTTG CGATCTGGAC TTTACGGATC TGAGGGATGA TGACGAGAAG 
GATGTGCTGG CCCCTCGAAG TTTGGGTGCC GGTATACCCC CGCCGCCTCC CCCGCTGGGT 
GGAGCCATTG CCCCACCGCC AATGATGCCT CCAAGCTTGG CACCACCACC CATGTACGGA 
TATGGCGGTA GTCTAACCAA TAGTGTCAAT TCGTTAAATG GTTCTATAAA TGGTGAACTG 
GTCAATGGTA ATAACACCAT CAAAAAGAAC AAGAAAACGG TCAAGCTCTT CTGGAAGGAA 
GTGCGCGAGG ACATGATACC GCAGGTGGTG GGCAAGACCA TATGGGATGA GCTGCCCGAT 
GCCAACGTGG ACACACAGAA GCTCGAACAT CTCTTTGAGT CCCGGGCCAA AGACTTGATG 
ACCAAGAAAC AACAAGAGCT GAACAAGAGC AAAGAGATCA TCGTGCTTGA TCACAAGCGC 
TCCAATGCCA TCAATATTGC GATCACTAAG CTGCCTCCTC CGAGGGCCAT TAAGACGGCC 
ATCCTCAAAA TGGATGCCAC TGTGGTCACA CGGGAGGGAA TCGACAAGCT ACTCAATATG 
CTGCCCACTG ATGAGGAGCG TGGCAAGATC CAGGAGGCCC AGTTATCCAA CCCAGAGTTG 
CCCCTGGGCA GCGCCGAGCA GTTCCTTTTG ACCCTGGCCT CCATTTCGGA GCTGGAAGCT 
CGCCTGAAAT TGTGGGCTTT CCGTCTGGAC TTTGACAACA GTGAGAAAGA GATTGCGGAA 
CCGCTGATGG ACCTCAAGCA AGGCATTGAG ATCCTGCGTC AGAATCGCAC CTTCCGCAGC 
ATCCTCTCTA CTCTGCTGTC TGTGGGCATC TTTTTGAATG GAGCTCCCGT CAAGGGATTC 
CAGATCGAGT ATCTGGCAAA GGTTCCGGAA GTGAAGGACA CGGTGCACAA GCACTCGCTG 
CTGCACCACC TCTGCCACAT GGTCATGGAG TCGAGCAGTG ATACTAGTGA TCTGTACTCC 
GAGATCGGAC CCATTACGCG AGCCTCCAAG GCGGATTTCA CGGACTTGGC ACACAATCTC 
AATCAGTTGG AGGCGGAGTG CAAGGCATGC TGGGATCGTC TGAAACTTAT TGCCAAACAC 
GACTGCCCGC CACCGTTAAA GCAGAAGCTG GTGGATTTCT TGGCCGATTG TGCGGAGCGC 
ATCATCATCC TGCAAATTGT CCATCGACGG GTGATGAATC GCTACCGGAA GTTCCTTTTG 
TGGCTGGGAA TGCCGCAGCA CAGTGTCGCG GAATCGCGTC CCAACGAATT CTGCCGGACA 
CTCTCCGAGT TCGCCCTGGA ATACCGCACC ACTAGAGAGC GGGTTCAGCA GCAGCTGGAG 
AAGAAGGCCA ACCACCGGGA ACGGAACAAG ACCCGCGGCA AACTGATCAT CGATATGGCC 
AAGTTCAAGA CCAAAGATGA CGTGGCCGAT GACGAGTTGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729411.1
CDS	321..4205
Translation	MIVKMEPDELITFRVQYLVDSDPLSACTAFPIPVRAPTYAFATTMPLANQLGTILRLLNAPQSIDDAAIQVYKDGDYGAYLDLESSLAEQAEEIEGVNVSRKNSLVVRTQLNVRVQAIIEKLLSAQGSDLRRALFSLKQVFQEDKDLVHAFVALGGLNCLVRVGNCADQNYQNYILRALGQVMLYVDGMNGVMKHEPTMQWLYSLIASNYRSVVKTALKLLLVFVEYAESNCYVLVSAIHAVDASQGTLPWSNIMRLLKDYDNADAELVIYATSLINKTLAGLNDQDSFYDESDLLEQQGMETVIQRYMSKPGTDLDLLDQLQLYEAVLKFEDGESDGQRLPQNSMRKTQRYRPGANTTERRKSRRHSTDNSPASLTKVLPTTVLRMTPTQATVDEDSSGSTNSTEFSGGLFNEKKPRDGAGVTPGLRRRRERAERHKSFLKEQQEAAANGIFAALEQREGLGQIVTAIPATIQTGDSPPQSLQHEVPPCNNISGITNISNNNVSNLSCNDTPNNSLLVMSPSKQLLTGSHSISIINNSFDKASNNNQSEFLTKKNLFRERNSTVINGIMKNIQQTDSAYKKYENEANRLNNYYSQSPKHQIHQPIQPTPEVLLSPKKTLNASGFDFTPAPLSSTPVKCDVPPEPEPLSPVKKPAAAPPPPPPPPPPPPPPPGWWHAPIYNSPQARTLTSTNPELLPHPVDPSDMESEDQQLLLQLKRDHTVKDLTQKLSNLPMSPTQDSANRALVGDMSGLISKAKEGLAKSKSKGEISRSSSVDQDVKKSEPKKSENELHWEELVRNMTRPLNLCDLDFTDLRDDDEKDVLAPRSLGAGIPPPPPPLGGAIAPPPMMPPSLAPPPMYGYGGSLTNSVNSLNGSINGELVNGNNTIKKNKKTVKLFWKEVREDMIPQVVGKTIWDELPDANVDTQKLEHLFESRAKDLMTKKQQELNKSKEIIVLDHKRSNAINIAITKLPPPRAIKTAILKMDATVVTREGIDKLLNMLPTDEERGKIQEAQLSNPELPLGSAEQFLLTLASISELEARLKLWAFRLDFDNSEKEIAEPLMDLKQGIEILRQNRTFRSILSTLLSVGIFLNGAPVKGFQIEYLAKVPEVKDTVHKHSLLHHLCHMVMESSSDTSDLYSEIGPITRASKADFTDLAHNLNQLEAECKACWDRLKLIAKHDCPPPLKQKLVDFLADCAERIIILQIVHRRVMNRYRKFLLWLGMPQHSVAESRPNEFCRTLSEFALEYRTTRERVQQQLEKKANHRERNKTRGKLIIDMAKFKTKDDVADDELK

Target ORF information:

RefSeq Version	NM_168294.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster formin homology 2 domain containing, transcript variant B (Fhos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168294.3

ORF Insert Sequence:

ATGATTGTGA AAATGGAGCC GGACGAACTG ATCACATTCC GTGTCCAGTA TCTGGTGGAC 
AGCGATCCGC TCTCGGCGTG CACCGCCTTC CCGATCCCGG TGCGGGCGCC CACCTACGCC 
TTCGCCACCA CCATGCCGCT GGCCAATCAG CTGGGCACAA TCCTGCGGCT GCTGAATGCA 
CCACAAAGCA TCGACGATGC AGCCATTCAG GTGTACAAGG ATGGCGACTA CGGAGCCTAC 
TTGGATCTGG AGTCATCGCT GGCCGAACAG GCCGAGGAAA TCGAGGGAGT CAACGTCAGC 
CGCAAAAACT CTCTGGTGGT GCGAACGCAG TTAAATGTGC GCGTACAAGC CATAATTGAG 
AAACTGCTCT CCGCACAGGG CAGTGACCTG CGGCGTGCTC TCTTCTCGCT GAAGCAGGTG 
TTCCAGGAGG ATAAGGATCT GGTGCACGCC TTCGTGGCCC TCGGCGGCCT CAATTGCCTG 
GTGCGCGTGG GCAATTGTGC GGATCAAAAC TATCAGAATT ACATTCTGCG AGCCCTCGGT 
CAGGTCATGC TCTATGTGGA CGGAATGAAC GGCGTGATGA AGCATGAGCC GACGATGCAG 
TGGCTCTACT CGCTGATTGC CTCCAATTAC CGATCCGTGG TGAAGACCGC CCTCAAGTTG 
TTGCTGGTCT TCGTGGAGTA CGCCGAGTCC AACTGCTATG TGCTGGTCAG TGCCATTCAC 
GCAGTGGATG CTTCCCAGGG CACACTGCCC TGGTCAAATA TAATGAGACT GCTTAAGGAC 
TATGACAATG CTGATGCCGA ACTGGTGATC TATGCCACTT CGCTGATTAA CAAAACTCTG 
GCGGGTCTGA ATGATCAGGA CAGCTTCTAC GACGAAAGCG ATCTTCTAGA GCAGCAGGGC 
ATGGAGACTG TGATCCAGCG TTATATGTCC AAGCCGGGCA CTGATTTGGA TCTGCTCGAT 
CAGTTGCAAC TGTATGAGGC GGTTCTCAAA TTTGAGGATG GTGAGTCGGA TGGACAGCGT 
TTGCCGCAGA ACTCAATGCG AAAAACACAA CGCTATCGTC CGGGAGCAAA TACCACTGAA 
AGACGCAAGT CGCGTCGCCA TAGCACAGAT AATAGTCCCG CATCACTCAC AAAGGTCCTG 
CCCACCACTG TCCTCCGGAT GACACCTACA CAAGCTACGG TGGATGAGGA TAGTTCTGGC 
TCCACCAACT CAACGGAATT TAGTGGCGGT CTCTTTAATG AGAAGAAACC TCGTGATGGT 
GCAGGCGTGA CACCAGGACT TCGAAGAAGA CGTGAGCGGG CGGAGCGGCA TAAGAGCTTT 
CTGAAGGAGC AACAGGAGGC GGCGGCCAAT GGAATCTTTG CTGCTCTCGA GCAGCGGGAG 
GGACTGGGCC AGATTGTAAC CGCCATACCC GCTACCATTC AAACCGGCGA TAGTCCGCCT 
CAGTCCTTGC AACATGAGGT GCCACCGTGC AACAACATCA GCGGAATCAC CAACATTAGT 
AACAACAATG TCAGCAACCT TAGCTGCAAT GACACGCCGA ACAACAGCCT CTTGGTGATG 
AGTCCTAGCA AGCAACTGCT CACTGGAAGC CACAGCATCT CCATTATTAA CAACAGCTTC 
GATAAGGCGA GCAATAACAA TCAGAGCGAG TTCCTGACCA AGAAGAACCT ATTCCGGGAA 
CGCAATTCAA CTGTGATCAA TGGTATTATG AAGAATATCC AGCAAACCGA TAGTGCCTAT 
AAGAAGTACG AGAACGAAGC GAACCGGCTG AACAACTATT ACAGTCAGTC GCCGAAACAT 
CAGATCCATC AGCCCATTCA GCCAACGCCG GAAGTCCTTT TGAGTCCCAA AAAAACGCTA 
AATGCCTCTG GGTTTGATTT CACGCCCGCT CCCCTCAGTT CCACCCCGGT TAAGTGTGAT 
GTCCCGCCGG AACCGGAGCC ACTGAGTCCG GTAAAGAAAC CCGCTGCGGC ACCACCACCG 
CCTCCTCCAC CGCCACCGCC GCCACCACCA CCACCTGGTT GGTGGCACGC CCCCATCTAC 
AACAGCCCGC AAGCTCGAAC CCTAACCAGT ACTAACCCAG AGCTGCTGCC GCATCCTGTG 
GATCCTTCAG ATATGGAAAG CGAGGATCAG CAGCTGCTCC TCCAGCTGAA GCGGGACCAC 
ACGGTCAAGG ATCTCACCCA GAAGTTGAGC AACCTGCCAA TGAGTCCCAC TCAGGATTCG 
GCGAATCGCG CCCTCGTCGG CGATATGTCC GGTCTGATTT CGAAAGCCAA AGAGGGTCTG 
GCCAAGAGCA AGAGCAAGGG AGAGATATCC AGATCTTCTA GTGTCGATCA GGATGTAAAG 
AAGTCTGAGC CCAAGAAGTC GGAAAATGAA CTTCACTGGG AGGAACTGGT TAGGAATATG 
ACAAGGCCCC TAAATCTTTG CGATCTGGAC TTTACGGATC TGAGGGATGA TGACGAGAAG 
GATGTGCTGG CCCCTCGAAG TTTGGGTGCC GGTATACCCC CGCCGCCTCC CCCGCTGGGT 
GGAGCCATTG CCCCACCGCC AATGATGCCT CCAAGCTTGG CACCACCACC CATGTACGGA 
TATGGCGGTA GTCTAACCAA TAGTGTCAAT TCGTTAAATG GTTCTATAAA TGGTGAACTG 
GTCAATGGTA ATAACACCAT CAAAAAGAAC AAGAAAACGG TCAAGCTCTT CTGGAAGGAA 
GTGCGCGAGG ACATGATACC GCAGGTGGTG GGCAAGACCA TATGGGATGA GCTGCCCGAT 
GCCAACGTGG ACACACAGAA GCTCGAACAT CTCTTTGAGT CCCGGGCCAA AGACTTGATG 
ACCAAGAAAC AACAAGAGCT GAACAAGAGC AAAGAGATCA TCGTGCTTGA TCACAAGCGC 
TCCAATGCCA TCAATATTGC GATCACTAAG CTGCCTCCTC CGAGGGCCAT TAAGACGGCC 
ATCCTCAAAA TGGATGCCAC TGTGGTCACA CGGGAGGGAA TCGACAAGCT ACTCAATATG 
CTGCCCACTG ATGAGGAGCG TGGCAAGATC CAGGAGGCCC AGTTATCCAA CCCAGAGTTG 
CCCCTGGGCA GCGCCGAGCA GTTCCTTTTG ACCCTGGCCT CCATTTCGGA GCTGGAAGCT 
CGCCTGAAAT TGTGGGCTTT CCGTCTGGAC TTTGACAACA GTGAGAAAGA GATTGCGGAA 
CCGCTGATGG ACCTCAAGCA AGGCATTGAG ATCCTGCGTC AGAATCGCAC CTTCCGCAGC 
ATCCTCTCTA CTCTGCTGTC TGTGGGCATC TTTTTGAATG GAGCTCCCGT CAAGGGATTC 
CAGATCGAGT ATCTGGCAAA GGTTCCGGAA GTGAAGGACA CGGTGCACAA GCACTCGCTG 
CTGCACCACC TCTGCCACAT GGTCATGGAG TCGAGCAGTG ATACTAGTGA TCTGTACTCC 
GAGATCGGAC CCATTACGCG AGCCTCCAAG GCGGATTTCA CGGACTTGGC ACACAATCTC 
AATCAGTTGG AGGCGGAGTG CAAGGCATGC TGGGATCGTC TGAAACTTAT TGCCAAACAC 
GACTGCCCGC CACCGTTAAA GCAGAAGCTG GTGGATTTCT TGGCCGATTG TGCGGAGCGC 
ATCATCATCC TGCAAATTGT CCATCGACGG GTGATGAATC GCTACCGGAA GTTCCTTTTG 
TGGCTGGGAA TGCCGCAGCA CAGTGTCGCG GAATCGCGTC CCAACGAATT CTGCCGGACA 
CTCTCCGAGT TCGCCCTGGA ATACCGCACC ACTAGAGAGC GGGTTCAGCA GCAGCTGGAG 
AAGAAGGCCA ACCACCGGGA ACGGAACAAG ACCCGCGGCA AACTGATCAT CGATATGGCC 
AAGTTCAAGA CCAAAGATGA CGTGGCCGAT GACGAGTTGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhos ( NM_001259754.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246683.1 Drosophila melanogaster formin homology 2 domain containing, transcript variant G (Fhos), mRNA.

Price & Availability↑

CloneID	OFa20761
Clone ID Related Accession (Same CDS sequence)	NM_001259754.2
Accession Version	NM_001259754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	formin homology 2 domain containing, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259754.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGTGA AAATGGAGCC GGACGAACTG ATCACATTCC GTGTCCAGTA TCTGGTGGAC 
AGCGATCCGC TCTCGGCGTG CACCGCCTTC CCGATCCCGG TGCGGGCGCC CACCTACGCC 
TTCGCCACCA CCATGCCGCT GGCCAATCAG CTGGGCACAA TCCTGCGGCT GCTGAATGCA 
CCACAAAGCA TCGACGATGC AGCCATTCAG GTGTACAAGG ATGGCGACTA CGGAGCCTAC 
TTGGATCTGG AGTCATCGCT GGCCGAACAG GCCGAGGAAA TCGAGGGAGT CAACGTCAGC 
CGCAAAAACT CTCTGGTGGT GCGAACGCAG TTAAATGTGC GCGTACAAGC CATAATTGAG 
AAACTGCTCT CCGCACAGGG CAGTGACCTG CGGCGTGCTC TCTTCTCGCT GAAGCAGGTG 
TTCCAGGAGG ATAAGGATCT GGTGCACGCC TTCGTGGCCC TCGGCGGCCT CAATTGCCTG 
GTGCGCGTGG GCAATTGTGC GGATCAAAAC TATCAGAATT ACATTCTGCG AGCCCTCGGT 
CAGGTCATGC TCTATGTGGA CGGAATGAAC GGCGTGATGA AGCATGAGCC GACGATGCAG 
TGGCTCTACT CGCTGATTGC CTCCAATTAC CGATCCGTGG TGAAGACCGC CCTCAAGTTG 
TTGCTGGTCT TCGTGGAGTA CGCCGAGTCC AACTGCTATG TGCTGGTCAG TGCCATTCAC 
GCAGTGGATG CTTCCCAGGG CACACTGCCC TGGTCAAATA TAATGAGACT GCTTAAGGAC 
TATGACAATG CTGATGCCGA ACTGGTGATC TATGCCACTT CGCTGATTAA CAAAACTCTG 
GCGGGTCTGA ATGATCAGGA CAGCTTCTAC GACGAAAGCG ATCTTCTAGA GCAGCAGGGC 
ATGGAGACTG TGATCCAGCG TTATATGTCC AAGCCGGGCA CTGATTTGGA TCTGCTCGAT 
CAGTTGCAAC TGTATGAGGC GGTTCTCAAA TTTGAGGATG GTGAGTCGGA TGGACAGCGT 
TTGCCGCAGA ACTCAATGCG AAAAACACAA CGCTATCGTC CGGGAGCAAA TACCACTGAA 
AGACGCAAGT CGCGTCGCCA TAGCACAGAT AATAGTCCCG CATCACTCAC AAAGGTCCTG 
CCCACCACTG TCCTCCGGAT GACACCTACA CAAGCTACGG TGGATGAGGA TAGTTCTGGC 
TCCACCAACT CAACGGAATT TAGTGGCGGT CTCTTTAATG AGAAGAAACC TCGTGATGGT 
GCAGGCGTGA CACCAGGACT TCGAAGAAGA CGTGAGCGGG CGGAGCGGCA TAAGAGCTTT 
CTGAAGGAGC AACAGGAGGC GGCGGCCAAT GGAATCTTTG CTGCTCTCGA GCAGCGGGAG 
GGACTGGATA TGGAAAGCGA GGATCAGCAG CTGCTCCTCC AGCTGAAGCG GGACCACACG 
GTCAAGGATC TCACCCAGAA GTTGAGCAAC CTGCCAATGA GTCCCACTCA GGATTCGGCG 
AATCGCGCCC TCGTCGGCGA TATGTCCGGT CTGATTTCGA AAGCCAAAGA GGGTCTGGCC 
AAGAGCAAGA GCAAGGGAGA GATATCCAGA TCTTCTAGTG TCGATCAGGA TGTAAAGAAG 
TCTGAGCCCA AGAAGTCGGA AAATGAACTT CACTGGGAGG AACTGGTTAG GAATATGACA 
AGGCCCCTAA ATCTTTGCGA TCTGGACTTT ACGGATCTGA GGGATGATGA CGAGAAGGAT 
GTGCTGGCCC CTCGAAGTTT GGGTGCCGGT ATACCCCCGC CGCCTCCCCC GCTGGGTGGA 
GCCATTGCCC CACCGCCAAT GATGCCTCCA AGCTTGGCAC CACCACCCAT GTACGGATAT 
GGCGGTAGTC TAACCAATAG TGTCAATTCG TTAAATGGTT CTATAAATGG TGAACTGGTC 
AATGGTAATA ACACCATCAA AAAGAACAAG AAAACGGTCA AGCTCTTCTG GAAGGAAGTG 
CGCGAGGACA TGATACCGCA GGTGGTGGGC AAGACCATAT GGGATGAGCT GCCCGATGCC 
AACGTGGACA CACAGAAGCT CGAACATCTC TTTGAGTCCC GGGCCAAAGA CTTGATGACC 
AAGAAACAAC AAGAGCTGAA CAAGAGCAAA GAGATCATCG TGCTTGATCA CAAGCGCTCC 
AATGCCATCA ATATTGCGAT CACTAAGCTG CCTCCTCCGA GGGCCATTAA GACGGCCATC 
CTCAAAATGG ATGCCACTGT GGTCACACGG GAGGGAATCG ACAAGCTACT CAATATGCTG 
CCCACTGATG AGGAGCGTGG CAAGATCCAG GAGGCCCAGT TATCCAACCC AGAGTTGCCC 
CTGGGCAGCG CCGAGCAGTT CCTTTTGACC CTGGCCTCCA TTTCGGAGCT GGAAGCTCGC 
CTGAAATTGT GGGCTTTCCG TCTGGACTTT GACAACAGTG AGAAAGAGAT TGCGGAACCG 
CTGATGGACC TCAAGCAAGG CATTGAGATC CTGCGTCAGA ATCGCACCTT CCGCAGCATC 
CTCTCTACTC TGCTGTCTGT GGGCATCTTT TTGAATGGAG CTCCCGTCAA GGGATTCCAG 
ATCGAGTATC TGGCAAAGGT TCCGGAAGTG AAGGACACGG TGCACAAGCA CTCGCTGCTG 
CACCACCTCT GCCACATGGT CATGGAGTCG AGCAGTGATA CTAGTGATCT GTACTCCGAG 
ATCGGACCCA TTACGCGAGC CTCCAAGGCG GATTTCACGG ACTTGGCACA CAATCTCAAT 
CAGTTGGAGG CGGAGTGCAA GGCATGCTGG GATCGTCTGA AACTTATTGC CAAACACGAC 
TGCCCGCCAC CGTTAAAGCA GAAGCTGGTG GATTTCTTGG CCGATTGTGC GGAGCGCATC 
ATCATCCTGC AAATTGTCCA TCGACGGGTG ATGAATCGCT ACCGGAAGTT CCTTTTGTGG 
CTGGGAATGC CGCAGCACAG TGTCGCGGAA TCGCGTCCCA ACGAATTCTG CCGGACACTC 
TCCGAGTTCG CCCTGGAATA CCGCACCACT AGAGAGCGGG TTCAGCAGCA GCTGGAGAAG 
AAGGCCAACC ACCGGGAACG GAACAAGACC CGCGGCAAAC TGATCATCGA TATGGCCAAG 
TTCAAGACCA AAGATGACGT GGCCGATGAC GAGTTGAAAA AACTTTTGGA CACATCGAGT 
GCCGACCAGG CCGATGGGAC ACTCACCTGG CGGAGGCGAC GAGCGGAACA GCTCCGCTCA 
CCAATTACCC GCCAAAGCGA GGAGCAGTTC ACCGATGGCG ACGATGAGAT ACTGGAATCT 
CTGGTTAAGA CCGCCACCAA GGCTCCAGGA ACACGGACAA CGCCGAGGGA ACGGAAACGG 
ACGCGCCACG CGGATCGCAA ATCATTACGT CGCACGCTGA AAAACGGTCT GACCGAGGAG 
GAGAAACAGC ACGTGGCTGC CTTGATACAA ACCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246683.1
CDS	321..3779
Translation	MIVKMEPDELITFRVQYLVDSDPLSACTAFPIPVRAPTYAFATTMPLANQLGTILRLLNAPQSIDDAAIQVYKDGDYGAYLDLESSLAEQAEEIEGVNVSRKNSLVVRTQLNVRVQAIIEKLLSAQGSDLRRALFSLKQVFQEDKDLVHAFVALGGLNCLVRVGNCADQNYQNYILRALGQVMLYVDGMNGVMKHEPTMQWLYSLIASNYRSVVKTALKLLLVFVEYAESNCYVLVSAIHAVDASQGTLPWSNIMRLLKDYDNADAELVIYATSLINKTLAGLNDQDSFYDESDLLEQQGMETVIQRYMSKPGTDLDLLDQLQLYEAVLKFEDGESDGQRLPQNSMRKTQRYRPGANTTERRKSRRHSTDNSPASLTKVLPTTVLRMTPTQATVDEDSSGSTNSTEFSGGLFNEKKPRDGAGVTPGLRRRRERAERHKSFLKEQQEAAANGIFAALEQREGLDMESEDQQLLLQLKRDHTVKDLTQKLSNLPMSPTQDSANRALVGDMSGLISKAKEGLAKSKSKGEISRSSSVDQDVKKSEPKKSENELHWEELVRNMTRPLNLCDLDFTDLRDDDEKDVLAPRSLGAGIPPPPPPLGGAIAPPPMMPPSLAPPPMYGYGGSLTNSVNSLNGSINGELVNGNNTIKKNKKTVKLFWKEVREDMIPQVVGKTIWDELPDANVDTQKLEHLFESRAKDLMTKKQQELNKSKEIIVLDHKRSNAINIAITKLPPPRAIKTAILKMDATVVTREGIDKLLNMLPTDEERGKIQEAQLSNPELPLGSAEQFLLTLASISELEARLKLWAFRLDFDNSEKEIAEPLMDLKQGIEILRQNRTFRSILSTLLSVGIFLNGAPVKGFQIEYLAKVPEVKDTVHKHSLLHHLCHMVMESSSDTSDLYSEIGPITRASKADFTDLAHNLNQLEAECKACWDRLKLIAKHDCPPPLKQKLVDFLADCAERIIILQIVHRRVMNRYRKFLLWLGMPQHSVAESRPNEFCRTLSEFALEYRTTRERVQQQLEKKANHRERNKTRGKLIIDMAKFKTKDDVADDELKKLLDTSSADQADGTLTWRRRRAEQLRSPITRQSEEQFTDGDDEILESLVKTATKAPGTRTTPRERKRTRHADRKSLRRTLKNGLTEEEKQHVAALIQTY

Target ORF information:

RefSeq Version	NM_001259754.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster formin homology 2 domain containing, transcript variant G (Fhos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259754.2

ORF Insert Sequence:

ATGATTGTGA AAATGGAGCC GGACGAACTG ATCACATTCC GTGTCCAGTA TCTGGTGGAC 
AGCGATCCGC TCTCGGCGTG CACCGCCTTC CCGATCCCGG TGCGGGCGCC CACCTACGCC 
TTCGCCACCA CCATGCCGCT GGCCAATCAG CTGGGCACAA TCCTGCGGCT GCTGAATGCA 
CCACAAAGCA TCGACGATGC AGCCATTCAG GTGTACAAGG ATGGCGACTA CGGAGCCTAC 
TTGGATCTGG AGTCATCGCT GGCCGAACAG GCCGAGGAAA TCGAGGGAGT CAACGTCAGC 
CGCAAAAACT CTCTGGTGGT GCGAACGCAG TTAAATGTGC GCGTACAAGC CATAATTGAG 
AAACTGCTCT CCGCACAGGG CAGTGACCTG CGGCGTGCTC TCTTCTCGCT GAAGCAGGTG 
TTCCAGGAGG ATAAGGATCT GGTGCACGCC TTCGTGGCCC TCGGCGGCCT CAATTGCCTG 
GTGCGCGTGG GCAATTGTGC GGATCAAAAC TATCAGAATT ACATTCTGCG AGCCCTCGGT 
CAGGTCATGC TCTATGTGGA CGGAATGAAC GGCGTGATGA AGCATGAGCC GACGATGCAG 
TGGCTCTACT CGCTGATTGC CTCCAATTAC CGATCCGTGG TGAAGACCGC CCTCAAGTTG 
TTGCTGGTCT TCGTGGAGTA CGCCGAGTCC AACTGCTATG TGCTGGTCAG TGCCATTCAC 
GCAGTGGATG CTTCCCAGGG CACACTGCCC TGGTCAAATA TAATGAGACT GCTTAAGGAC 
TATGACAATG CTGATGCCGA ACTGGTGATC TATGCCACTT CGCTGATTAA CAAAACTCTG 
GCGGGTCTGA ATGATCAGGA CAGCTTCTAC GACGAAAGCG ATCTTCTAGA GCAGCAGGGC 
ATGGAGACTG TGATCCAGCG TTATATGTCC AAGCCGGGCA CTGATTTGGA TCTGCTCGAT 
CAGTTGCAAC TGTATGAGGC GGTTCTCAAA TTTGAGGATG GTGAGTCGGA TGGACAGCGT 
TTGCCGCAGA ACTCAATGCG AAAAACACAA CGCTATCGTC CGGGAGCAAA TACCACTGAA 
AGACGCAAGT CGCGTCGCCA TAGCACAGAT AATAGTCCCG CATCACTCAC AAAGGTCCTG 
CCCACCACTG TCCTCCGGAT GACACCTACA CAAGCTACGG TGGATGAGGA TAGTTCTGGC 
TCCACCAACT CAACGGAATT TAGTGGCGGT CTCTTTAATG AGAAGAAACC TCGTGATGGT 
GCAGGCGTGA CACCAGGACT TCGAAGAAGA CGTGAGCGGG CGGAGCGGCA TAAGAGCTTT 
CTGAAGGAGC AACAGGAGGC GGCGGCCAAT GGAATCTTTG CTGCTCTCGA GCAGCGGGAG 
GGACTGGATA TGGAAAGCGA GGATCAGCAG CTGCTCCTCC AGCTGAAGCG GGACCACACG 
GTCAAGGATC TCACCCAGAA GTTGAGCAAC CTGCCAATGA GTCCCACTCA GGATTCGGCG 
AATCGCGCCC TCGTCGGCGA TATGTCCGGT CTGATTTCGA AAGCCAAAGA GGGTCTGGCC 
AAGAGCAAGA GCAAGGGAGA GATATCCAGA TCTTCTAGTG TCGATCAGGA TGTAAAGAAG 
TCTGAGCCCA AGAAGTCGGA AAATGAACTT CACTGGGAGG AACTGGTTAG GAATATGACA 
AGGCCCCTAA ATCTTTGCGA TCTGGACTTT ACGGATCTGA GGGATGATGA CGAGAAGGAT 
GTGCTGGCCC CTCGAAGTTT GGGTGCCGGT ATACCCCCGC CGCCTCCCCC GCTGGGTGGA 
GCCATTGCCC CACCGCCAAT GATGCCTCCA AGCTTGGCAC CACCACCCAT GTACGGATAT 
GGCGGTAGTC TAACCAATAG TGTCAATTCG TTAAATGGTT CTATAAATGG TGAACTGGTC 
AATGGTAATA ACACCATCAA AAAGAACAAG AAAACGGTCA AGCTCTTCTG GAAGGAAGTG 
CGCGAGGACA TGATACCGCA GGTGGTGGGC AAGACCATAT GGGATGAGCT GCCCGATGCC 
AACGTGGACA CACAGAAGCT CGAACATCTC TTTGAGTCCC GGGCCAAAGA CTTGATGACC 
AAGAAACAAC AAGAGCTGAA CAAGAGCAAA GAGATCATCG TGCTTGATCA CAAGCGCTCC 
AATGCCATCA ATATTGCGAT CACTAAGCTG CCTCCTCCGA GGGCCATTAA GACGGCCATC 
CTCAAAATGG ATGCCACTGT GGTCACACGG GAGGGAATCG ACAAGCTACT CAATATGCTG 
CCCACTGATG AGGAGCGTGG CAAGATCCAG GAGGCCCAGT TATCCAACCC AGAGTTGCCC 
CTGGGCAGCG CCGAGCAGTT CCTTTTGACC CTGGCCTCCA TTTCGGAGCT GGAAGCTCGC 
CTGAAATTGT GGGCTTTCCG TCTGGACTTT GACAACAGTG AGAAAGAGAT TGCGGAACCG 
CTGATGGACC TCAAGCAAGG CATTGAGATC CTGCGTCAGA ATCGCACCTT CCGCAGCATC 
CTCTCTACTC TGCTGTCTGT GGGCATCTTT TTGAATGGAG CTCCCGTCAA GGGATTCCAG 
ATCGAGTATC TGGCAAAGGT TCCGGAAGTG AAGGACACGG TGCACAAGCA CTCGCTGCTG 
CACCACCTCT GCCACATGGT CATGGAGTCG AGCAGTGATA CTAGTGATCT GTACTCCGAG 
ATCGGACCCA TTACGCGAGC CTCCAAGGCG GATTTCACGG ACTTGGCACA CAATCTCAAT 
CAGTTGGAGG CGGAGTGCAA GGCATGCTGG GATCGTCTGA AACTTATTGC CAAACACGAC 
TGCCCGCCAC CGTTAAAGCA GAAGCTGGTG GATTTCTTGG CCGATTGTGC GGAGCGCATC 
ATCATCCTGC AAATTGTCCA TCGACGGGTG ATGAATCGCT ACCGGAAGTT CCTTTTGTGG 
CTGGGAATGC CGCAGCACAG TGTCGCGGAA TCGCGTCCCA ACGAATTCTG CCGGACACTC 
TCCGAGTTCG CCCTGGAATA CCGCACCACT AGAGAGCGGG TTCAGCAGCA GCTGGAGAAG 
AAGGCCAACC ACCGGGAACG GAACAAGACC CGCGGCAAAC TGATCATCGA TATGGCCAAG 
TTCAAGACCA AAGATGACGT GGCCGATGAC GAGTTGAAAA AACTTTTGGA CACATCGAGT 
GCCGACCAGG CCGATGGGAC ACTCACCTGG CGGAGGCGAC GAGCGGAACA GCTCCGCTCA 
CCAATTACCC GCCAAAGCGA GGAGCAGTTC ACCGATGGCG ACGATGAGAT ACTGGAATCT 
CTGGTTAAGA CCGCCACCAA GGCTCCAGGA ACACGGACAA CGCCGAGGGA ACGGAAACGG 
ACGCGCCACG CGGATCGCAA ATCATTACGT CGCACGCTGA AAAACGGTCT GACCGAGGAG 
GAGAAACAGC ACGTGGCTGC CTTGATACAA ACCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhos ( NM_001259753.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246682.1 Drosophila melanogaster formin homology 2 domain containing, transcript variant F (Fhos), mRNA.

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CloneID	OFa20762
Clone ID Related Accession (Same CDS sequence)	NM_001259753.2
Accession Version	NM_001259753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	formin homology 2 domain containing, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259753.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCTATG TGGACGGAAT GAACGGCGTG ATGAAGCATG AGCCGACGAT GCAGTGGCTC 
TACTCGCTGA TTGCCTCCAA TTACCGATCC GTGGTGAAGA CCGCCCTCAA GTTGTTGCTG 
GTCTTCGTGG AGTACGCCGA GTCCAACTGC TATGTGCTGG TCAGTGCCAT TCACGCAGTG 
GATGCTTCCC AGGGCACACT GCCCTGGTCA AATATAATGA GACTGCTTAA GGACTATGAC 
AATGCTGATG CCGAACTGGT GATCTATGCC ACTTCGCTGA TTAACAAAAC TCTGGCGGGT 
CTGAATGATC AGGACAGCTT CTACGACGAA AGCGATCTTC TAGAGCAGCA GGGCATGGAG 
ACTGTGATCC AGCGTTATAT GTCCAAGCCG GGCACTGATT TGGATCTGCT CGATCAGTTG 
CAACTGTATG AGGCGGTTCT CAAATTTGAG GATGGTGAGT CGGATGGACA GCGTTTGCCG 
CAGAACTCAA TGCGAAAAAC ACAACGCTAT CGTCCGGGAG CAAATACCAC TGAAAGACGC 
AAGTCGCGTC GCCATAGCAC AGATAATAGT CCCGCATCAC TCACAAAGGT CCTGCCCACC 
ACTGTCCTCC GGATGACACC TACACAAGCT ACGGTGGATG AGGATAGTTC TGGCTCCACC 
AACTCAACGG AATTTAGTGG CGGTCTCTTT AATGAGAAGA AACCTCGTGA TGGTGCAGGC 
GTGACACCAG GACTTCGAAG AAGACGTGAG CGGGCGGAGC GGCATAAGAG CTTTCTGAAG 
GAGCAACAGG AGGCGGCGGC CAATGGAATC TTTGCTGCTC TCGAGCAGCG GGAGGGACTG 
GGCCAGATTG TAACCGCCAT ACCCGCTACC ATTCAAACCG GCGATAGTCC GCCTCAGTCC 
TTGCAACATG AGGTGCCACC GTGCAACAAC ATCAGCGGAA TCACCAACAT TAGTAACAAC 
AATGTCAGCA ACCTTAGCTG CAATGACACG CCGAACAACA GCCTCTTGGT GATGAGTCCT 
AGCAAGCAAC TGCTCACTGG AAGCCACAGC ATCTCCATTA TTAACAACAG CTTCGATAAG 
GCGAGCAATA ACAATCAGAG CGAGTTCCTG ACCAAGAAGA ACCTATTCCG GGAACGCAAT 
TCAACTGTGA TCAATGGTAT TATGAAGAAT ATCCAGCAAA CCGATAGTGC CTATAAGAAG 
TACGAGAACG AAGCGAACCG GCTGAACAAC TATTACAGTC AGTCGCCGAA ACATCAGATC 
CATCAGCCCA TTCAGCCAAC GCCGGAAGTC CTTTTGAGTC CCAAAAAAAC GCTAAATGCC 
TCTGGGTTTG ATTTCACGCC CGCTCCCCTC AGTTCCACCC CGGTTAAGTG TGATGTCCCG 
CCGGAACCGG AGCCACTGAG TCCGGTAAAG AAACCCGCTG CGGCACCACC ACCGCCTCCT 
CCACCGCCAC CGCCGCCACC ACCACCACCT GGTTGGTGGC ACGCCCCCAT CTACAACAGC 
CCGCAAGCTC GAACCCTAAC CAGTACTAAC CCAGAGCTGC TGCCGCATCC TGTGGATCCT 
TCAGATATGG AAAGCGAGGA TCAGCAGCTG CTCCTCCAGC TGAAGCGGGA CCACACGGTC 
AAGGATCTCA CCCAGAAGTT GAGCAACCTG CCAATGAGTC CCACTCAGGA TTCGGCGAAT 
CGCGCCCTCG TCGGCGATAT GTCCGGTCTG ATTTCGAAAG CCAAAGAGGG TCTGGCCAAG 
AGCAAGAGCA AGGGAGAGAT ATCCAGATCT TCTAGTGTCG ATCAGGATGT AAAGAAGTCT 
GAGCCCAAGA AGTCGGAAAA TGAACTTCAC TGGGAGGAAC TGGTTAGGAA TATGACAAGG 
CCCCTAAATC TTTGCGATCT GGACTTTACG GATCTGAGGG ATGATGACGA GAAGGATGTG 
CTGGCCCCTC GAAGTTTGGG TGCCGGTATA CCCCCGCCGC CTCCCCCGCT GGGTGGAGCC 
ATTGCCCCAC CGCCAATGAT GCCTCCAAGC TTGGCACCAC CACCCATGTA CGGATATGGC 
GGTAGTCTAA CCAATAGTGT CAATTCGTTA AATGGTTCTA TAAATGGTGA ACTGGTCAAT 
GGTAATAACA CCATCAAAAA GAACAAGAAA ACGGTCAAGC TCTTCTGGAA GGAAGTGCGC 
GAGGACATGA TACCGCAGGT GGTGGGCAAG ACCATATGGG ATGAGCTGCC CGATGCCAAC 
GTGGACACAC AGAAGCTCGA ACATCTCTTT GAGTCCCGGG CCAAAGACTT GATGACCAAG 
AAACAACAAG AGCTGAACAA GAGCAAAGAG ATCATCGTGC TTGATCACAA GCGCTCCAAT 
GCCATCAATA TTGCGATCAC TAAGCTGCCT CCTCCGAGGG CCATTAAGAC GGCCATCCTC 
AAAATGGATG CCACTGTGGT CACACGGGAG GGAATCGACA AGCTACTCAA TATGCTGCCC 
ACTGATGAGG AGCGTGGCAA GATCCAGGAG GCCCAGTTAT CCAACCCAGA GTTGCCCCTG 
GGCAGCGCCG AGCAGTTCCT TTTGACCCTG GCCTCCATTT CGGAGCTGGA AGCTCGCCTG 
AAATTGTGGG CTTTCCGTCT GGACTTTGAC AACAGTGAGA AAGAGATTGC GGAACCGCTG 
ATGGACCTCA AGCAAGGCAT TGAGATCCTG CGTCAGAATC GCACCTTCCG CAGCATCCTC 
TCTACTCTGC TGTCTGTGGG CATCTTTTTG AATGGAGCTC CCGTCAAGGG ATTCCAGATC 
GAGTATCTGG CAAAGGTTCC GGAAGTGAAG GACACGGTGC ACAAGCACTC GCTGCTGCAC 
CACCTCTGCC ACATGGTCAT GGAGTCGAGC AGTGATACTA GTGATCTGTA CTCCGAGATC 
GGACCCATTA CGCGAGCCTC CAAGGCGGAT TTCACGGACT TGGCACACAA TCTCAATCAG 
TTGGAGGCGG AGTGCAAGGC ATGCTGGGAT CGTCTGAAAC TTATTGCCAA ACACGACTGC 
CCGCCACCGT TAAAGCAGAA GCTGGTGGAT TTCTTGGCCG ATTGTGCGGA GCGCATCATC 
ATCCTGCAAA TTGTCCATCG ACGGGTGATG AATCGCTACC GGAAGTTCCT TTTGTGGCTG 
GGAATGCCGC AGCACAGTGT CGCGGAATCG CGTCCCAACG AATTCTGCCG GACACTCTCC 
GAGTTCGCCC TGGAATACCG CACCACTAGA GAGCGGGTTC AGCAGCAGCT GGAGAAGAAG 
GCCAACCACC GGGAACGGAA CAAGACCCGC GGCAAACTGA TCATCGATAT GGCCAAGTTC 
AAGACCAAAG ATGACGTGGC CGATGACGAG TTGAAAAAAC TTTTGGACAC ATCGAGTGCC 
GACCAGGCCG ATGGGACACT CACCTGGCGG AGGCGACGAG CGGAACAGCT CCGCTCACCA 
ATTACCCGCC AAAGCGAGGA GCAGTTCACC GATGGCGACG ATGAGATACT GGAATCTCTG 
GTTAAGACCG CCACCAAGGC TCCAGGAACA CGGACAACGC CGAGGGAACG GAAACGGACG 
CGCCACGCGG ATCGCAAATC ATTGAAGCGC AGCCGCACGC GCGAAGGATT CACAGTTGAA 
AGAGCACCAT CGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246682.1
CDS	546..4163
Translation	MLYVDGMNGVMKHEPTMQWLYSLIASNYRSVVKTALKLLLVFVEYAESNCYVLVSAIHAVDASQGTLPWSNIMRLLKDYDNADAELVIYATSLINKTLAGLNDQDSFYDESDLLEQQGMETVIQRYMSKPGTDLDLLDQLQLYEAVLKFEDGESDGQRLPQNSMRKTQRYRPGANTTERRKSRRHSTDNSPASLTKVLPTTVLRMTPTQATVDEDSSGSTNSTEFSGGLFNEKKPRDGAGVTPGLRRRRERAERHKSFLKEQQEAAANGIFAALEQREGLGQIVTAIPATIQTGDSPPQSLQHEVPPCNNISGITNISNNNVSNLSCNDTPNNSLLVMSPSKQLLTGSHSISIINNSFDKASNNNQSEFLTKKNLFRERNSTVINGIMKNIQQTDSAYKKYENEANRLNNYYSQSPKHQIHQPIQPTPEVLLSPKKTLNASGFDFTPAPLSSTPVKCDVPPEPEPLSPVKKPAAAPPPPPPPPPPPPPPPGWWHAPIYNSPQARTLTSTNPELLPHPVDPSDMESEDQQLLLQLKRDHTVKDLTQKLSNLPMSPTQDSANRALVGDMSGLISKAKEGLAKSKSKGEISRSSSVDQDVKKSEPKKSENELHWEELVRNMTRPLNLCDLDFTDLRDDDEKDVLAPRSLGAGIPPPPPPLGGAIAPPPMMPPSLAPPPMYGYGGSLTNSVNSLNGSINGELVNGNNTIKKNKKTVKLFWKEVREDMIPQVVGKTIWDELPDANVDTQKLEHLFESRAKDLMTKKQQELNKSKEIIVLDHKRSNAINIAITKLPPPRAIKTAILKMDATVVTREGIDKLLNMLPTDEERGKIQEAQLSNPELPLGSAEQFLLTLASISELEARLKLWAFRLDFDNSEKEIAEPLMDLKQGIEILRQNRTFRSILSTLLSVGIFLNGAPVKGFQIEYLAKVPEVKDTVHKHSLLHHLCHMVMESSSDTSDLYSEIGPITRASKADFTDLAHNLNQLEAECKACWDRLKLIAKHDCPPPLKQKLVDFLADCAERIIILQIVHRRVMNRYRKFLLWLGMPQHSVAESRPNEFCRTLSEFALEYRTTRERVQQQLEKKANHRERNKTRGKLIIDMAKFKTKDDVADDELKKLLDTSSADQADGTLTWRRRRAEQLRSPITRQSEEQFTDGDDEILESLVKTATKAPGTRTTPRERKRTRHADRKSLKRSRTREGFTVERAPSP

Target ORF information:

RefSeq Version	NM_001259753.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster formin homology 2 domain containing, transcript variant F (Fhos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259753.2

ORF Insert Sequence:

ATGCTCTATG TGGACGGAAT GAACGGCGTG ATGAAGCATG AGCCGACGAT GCAGTGGCTC 
TACTCGCTGA TTGCCTCCAA TTACCGATCC GTGGTGAAGA CCGCCCTCAA GTTGTTGCTG 
GTCTTCGTGG AGTACGCCGA GTCCAACTGC TATGTGCTGG TCAGTGCCAT TCACGCAGTG 
GATGCTTCCC AGGGCACACT GCCCTGGTCA AATATAATGA GACTGCTTAA GGACTATGAC 
AATGCTGATG CCGAACTGGT GATCTATGCC ACTTCGCTGA TTAACAAAAC TCTGGCGGGT 
CTGAATGATC AGGACAGCTT CTACGACGAA AGCGATCTTC TAGAGCAGCA GGGCATGGAG 
ACTGTGATCC AGCGTTATAT GTCCAAGCCG GGCACTGATT TGGATCTGCT CGATCAGTTG 
CAACTGTATG AGGCGGTTCT CAAATTTGAG GATGGTGAGT CGGATGGACA GCGTTTGCCG 
CAGAACTCAA TGCGAAAAAC ACAACGCTAT CGTCCGGGAG CAAATACCAC TGAAAGACGC 
AAGTCGCGTC GCCATAGCAC AGATAATAGT CCCGCATCAC TCACAAAGGT CCTGCCCACC 
ACTGTCCTCC GGATGACACC TACACAAGCT ACGGTGGATG AGGATAGTTC TGGCTCCACC 
AACTCAACGG AATTTAGTGG CGGTCTCTTT AATGAGAAGA AACCTCGTGA TGGTGCAGGC 
GTGACACCAG GACTTCGAAG AAGACGTGAG CGGGCGGAGC GGCATAAGAG CTTTCTGAAG 
GAGCAACAGG AGGCGGCGGC CAATGGAATC TTTGCTGCTC TCGAGCAGCG GGAGGGACTG 
GGCCAGATTG TAACCGCCAT ACCCGCTACC ATTCAAACCG GCGATAGTCC GCCTCAGTCC 
TTGCAACATG AGGTGCCACC GTGCAACAAC ATCAGCGGAA TCACCAACAT TAGTAACAAC 
AATGTCAGCA ACCTTAGCTG CAATGACACG CCGAACAACA GCCTCTTGGT GATGAGTCCT 
AGCAAGCAAC TGCTCACTGG AAGCCACAGC ATCTCCATTA TTAACAACAG CTTCGATAAG 
GCGAGCAATA ACAATCAGAG CGAGTTCCTG ACCAAGAAGA ACCTATTCCG GGAACGCAAT 
TCAACTGTGA TCAATGGTAT TATGAAGAAT ATCCAGCAAA CCGATAGTGC CTATAAGAAG 
TACGAGAACG AAGCGAACCG GCTGAACAAC TATTACAGTC AGTCGCCGAA ACATCAGATC 
CATCAGCCCA TTCAGCCAAC GCCGGAAGTC CTTTTGAGTC CCAAAAAAAC GCTAAATGCC 
TCTGGGTTTG ATTTCACGCC CGCTCCCCTC AGTTCCACCC CGGTTAAGTG TGATGTCCCG 
CCGGAACCGG AGCCACTGAG TCCGGTAAAG AAACCCGCTG CGGCACCACC ACCGCCTCCT 
CCACCGCCAC CGCCGCCACC ACCACCACCT GGTTGGTGGC ACGCCCCCAT CTACAACAGC 
CCGCAAGCTC GAACCCTAAC CAGTACTAAC CCAGAGCTGC TGCCGCATCC TGTGGATCCT 
TCAGATATGG AAAGCGAGGA TCAGCAGCTG CTCCTCCAGC TGAAGCGGGA CCACACGGTC 
AAGGATCTCA CCCAGAAGTT GAGCAACCTG CCAATGAGTC CCACTCAGGA TTCGGCGAAT 
CGCGCCCTCG TCGGCGATAT GTCCGGTCTG ATTTCGAAAG CCAAAGAGGG TCTGGCCAAG 
AGCAAGAGCA AGGGAGAGAT ATCCAGATCT TCTAGTGTCG ATCAGGATGT AAAGAAGTCT 
GAGCCCAAGA AGTCGGAAAA TGAACTTCAC TGGGAGGAAC TGGTTAGGAA TATGACAAGG 
CCCCTAAATC TTTGCGATCT GGACTTTACG GATCTGAGGG ATGATGACGA GAAGGATGTG 
CTGGCCCCTC GAAGTTTGGG TGCCGGTATA CCCCCGCCGC CTCCCCCGCT GGGTGGAGCC 
ATTGCCCCAC CGCCAATGAT GCCTCCAAGC TTGGCACCAC CACCCATGTA CGGATATGGC 
GGTAGTCTAA CCAATAGTGT CAATTCGTTA AATGGTTCTA TAAATGGTGA ACTGGTCAAT 
GGTAATAACA CCATCAAAAA GAACAAGAAA ACGGTCAAGC TCTTCTGGAA GGAAGTGCGC 
GAGGACATGA TACCGCAGGT GGTGGGCAAG ACCATATGGG ATGAGCTGCC CGATGCCAAC 
GTGGACACAC AGAAGCTCGA ACATCTCTTT GAGTCCCGGG CCAAAGACTT GATGACCAAG 
AAACAACAAG AGCTGAACAA GAGCAAAGAG ATCATCGTGC TTGATCACAA GCGCTCCAAT 
GCCATCAATA TTGCGATCAC TAAGCTGCCT CCTCCGAGGG CCATTAAGAC GGCCATCCTC 
AAAATGGATG CCACTGTGGT CACACGGGAG GGAATCGACA AGCTACTCAA TATGCTGCCC 
ACTGATGAGG AGCGTGGCAA GATCCAGGAG GCCCAGTTAT CCAACCCAGA GTTGCCCCTG 
GGCAGCGCCG AGCAGTTCCT TTTGACCCTG GCCTCCATTT CGGAGCTGGA AGCTCGCCTG 
AAATTGTGGG CTTTCCGTCT GGACTTTGAC AACAGTGAGA AAGAGATTGC GGAACCGCTG 
ATGGACCTCA AGCAAGGCAT TGAGATCCTG CGTCAGAATC GCACCTTCCG CAGCATCCTC 
TCTACTCTGC TGTCTGTGGG CATCTTTTTG AATGGAGCTC CCGTCAAGGG ATTCCAGATC 
GAGTATCTGG CAAAGGTTCC GGAAGTGAAG GACACGGTGC ACAAGCACTC GCTGCTGCAC 
CACCTCTGCC ACATGGTCAT GGAGTCGAGC AGTGATACTA GTGATCTGTA CTCCGAGATC 
GGACCCATTA CGCGAGCCTC CAAGGCGGAT TTCACGGACT TGGCACACAA TCTCAATCAG 
TTGGAGGCGG AGTGCAAGGC ATGCTGGGAT CGTCTGAAAC TTATTGCCAA ACACGACTGC 
CCGCCACCGT TAAAGCAGAA GCTGGTGGAT TTCTTGGCCG ATTGTGCGGA GCGCATCATC 
ATCCTGCAAA TTGTCCATCG ACGGGTGATG AATCGCTACC GGAAGTTCCT TTTGTGGCTG 
GGAATGCCGC AGCACAGTGT CGCGGAATCG CGTCCCAACG AATTCTGCCG GACACTCTCC 
GAGTTCGCCC TGGAATACCG CACCACTAGA GAGCGGGTTC AGCAGCAGCT GGAGAAGAAG 
GCCAACCACC GGGAACGGAA CAAGACCCGC GGCAAACTGA TCATCGATAT GGCCAAGTTC 
AAGACCAAAG ATGACGTGGC CGATGACGAG TTGAAAAAAC TTTTGGACAC ATCGAGTGCC 
GACCAGGCCG ATGGGACACT CACCTGGCGG AGGCGACGAG CGGAACAGCT CCGCTCACCA 
ATTACCCGCC AAAGCGAGGA GCAGTTCACC GATGGCGACG ATGAGATACT GGAATCTCTG 
GTTAAGACCG CCACCAAGGC TCCAGGAACA CGGACAACGC CGAGGGAACG GAAACGGACG 
CGCCACGCGG ATCGCAAATC ATTGAAGCGC AGCCGCACGC GCGAAGGATT CACAGTTGAA 
AGAGCACCAT CGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhos ( NM_168293.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729410.1 Drosophila melanogaster formin homology 2 domain containing, transcript variant A (Fhos), mRNA.

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CloneID	OFa17938
Clone ID Related Accession (Same CDS sequence)	NM_168293.3
Accession Version	NM_168293.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	formin homology 2 domain containing, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168293.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGTGA AAATGGAGCC GGACGAACTG ATCACATTCC GTGTCCAGTA TCTGGTGGAC 
AGCGATCCGC TCTCGGCGTG CACCGCCTTC CCGATCCCGG TGCGGGCGCC CACCTACGCC 
TTCGCCACCA CCATGCCGCT GGCCAATCAG CTGGGCACAA TCCTGCGGCT GCTGAATGCA 
CCACAAAGCA TCGACGATGC AGCCATTCAG GTGTACAAGG ATGGCGACTA CGGAGCCTAC 
TTGGATCTGG AGTCATCGCT GGCCGAACAG GCCGAGGAAA TCGAGGGAGT CAACGTCAGC 
CGCAAAAACT CTCTGGTGGT GCGAACGCAG TTAAATGTGC GCGTACAAGC CATAATTGAG 
AAACTGCTCT CCGCACAGGG CAGTGACCTG CGGCGTGCTC TCTTCTCGCT GAAGCAGGTG 
TTCCAGGAGG ATAAGGATCT GGTGCACGCC TTCGTGGCCC TCGGCGGCCT CAATTGCCTG 
GTGCGCGTGG GCAATTGTGC GGATCAAAAC TATCAGAATT ACATTCTGCG AGCCCTCGGT 
CAGGTCATGC TCTATGTGGA CGGAATGAAC GGCGTGATGA AGCATGAGCC GACGATGCAG 
TGGCTCTACT CGCTGATTGC CTCCAATTAC CGATCCGTGG TGAAGACCGC CCTCAAGTTG 
TTGCTGGTCT TCGTGGAGTA CGCCGAGTCC AACTGCTATG TGCTGGTCAG TGCCATTCAC 
GCAGTGGATG CTTCCCAGGG CACACTGCCC TGGTCAAATA TAATGAGACT GCTTAAGGAC 
TATGACAATG CTGATGCCGA ACTGGTGATC TATGCCACTT CGCTGATTAA CAAAACTCTG 
GCGGGTCTGA ATGATCAGGA CAGCTTCTAC GACGAAAGCG ATCTTCTAGA GCAGCAGGGC 
ATGGAGACTG TGATCCAGCG TTATATGTCC AAGCCGGGCA CTGATTTGGA TCTGCTCGAT 
CAGTTGCAAC TGTATGAGGC GGTTCTCAAA TTTGAGGATG GTGAGTCGGA TGGACAGCGT 
TTGCCGCAGA ACTCAATGCG AAAAACACAA CGCTATCGTC CGGGAGCAAA TACCACTGAA 
AGACGCAAGT CGCGTCGCCA TAGCACAGAT AATAGTCCCG CATCACTCAC AAAGGTCCTG 
CCCACCACTG TCCTCCGGAT GACACCTACA CAAGCTACGG TGGATGAGGA TAGTTCTGGC 
TCCACCAACT CAACGGAATT TAGTGGCGGT CTCTTTAATG AGAAGAAACC TCGTGATGGT 
GCAGGCGTGA CACCAGGACT TCGAAGAAGA CGTGAGCGGG CGGAGCGGCA TAAGAGCTTT 
CTGAAGGAGC AACAGGAGGC GGCGGCCAAT GGAATCTTTG CTGCTCTCGA GCAGCGGGAG 
GGACTGGGCC AGATTGTAAC CGCCATACCC GCTACCATTC AAACCGGCGA TAGTCCGCCT 
CAGTCCTTGC AACATGAGGT GCCACCGTGC AACAACATCA GCGGAATCAC CAACATTAGT 
AACAACAATG TCAGCAACCT TAGCTGCAAT GACACGCCGA ACAACAGCCT CTTGGTGATG 
AGTCCTAGCA AGCAACTGCT CACTGGAAGC CACAGCATCT CCATTATTAA CAACAGCTTC 
GATAAGGCGA GCAATAACAA TCAGAGCGAG TTCCTGACCA AGAAGAACCT ATTCCGGGAA 
CGCAATTCAA CTGTGATCAA TGGTATTATG AAGAATATCC AGCAAACCGA TAGTGCCTAT 
AAGAAGTACG AGAACGAAGC GAACCGGCTG AACAACTATT ACAGTCAGTC GCCGAAACAT 
CAGATCCATC AGCCCATTCA GCCAACGCCG GAAGTCCTTT TGAGTCCCAA AAAAACGCTA 
AATGCCTCTG GGTTTGATTT CACGCCCGCT CCCCTCAGTT CCACCCCGGT TAAGTGTGAT 
GTCCCGCCGG AACCGGAGCC ACTGAGTCCG GTAAAGAAAC CCGCTGCGGC ACCACCACCG 
CCTCCTCCAC CGCCACCGCC GCCACCACCA CCACCTGGTT GGTGGCACGC CCCCATCTAC 
AACAGCCCGC AAGCTCGAAC CCTAACCAGT ACTAACCCAG AGCTGCTGCC GCATCCTGTG 
GATCCTTCAG ATATGGAAAG CGAGGATCAG CAGCTGCTCC TCCAGCTGAA GCGGGACCAC 
ACGGTCAAGG ATCTCACCCA GAAGTTGAGC AACCTGCCAA TGAGTCCCAC TCAGGATTCG 
GCGAATCGCG CCCTCGTCGG CGATATGTCC GGTCTGATTT CGAAAGCCAA AGAGGGTCTG 
GCCAAGAGCA AGAGCAAGGG AGAGATATCC AGATCTTCTA GTGTCGATCA GGATGTAAAG 
AAGTCTGAGC CCAAGAAGTC GGAAAATGAA CTTCACTGGG AGGAACTGGT TAGGAATATG 
ACAAGGCCCC TAAATCTTTG CGATCTGGAC TTTACGGATC TGAGGGATGA TGACGAGAAG 
GATGTGCTGG CCCCTCGAAG TTTGGGTGCC GGTATACCCC CGCCGCCTCC CCCGCTGGGT 
GGAGCCATTG CCCCACCGCC AATGATGCCT CCAAGCTTGG CACCACCACC CATGTACGGA 
TATGGCGGTA GTCTAACCAA TAGTGTCAAT TCGTTAAATG GTTCTATAAA TGGTGAACTG 
GTCAATGGTA ATAACACCAT CAAAAAGAAC AAGAAAACGG TCAAGCTCTT CTGGAAGGAA 
GTGCGCGAGG ACATGATACC GCAGGTGGTG GGCAAGACCA TATGGGATGA GCTGCCCGAT 
GCCAACGTGG ACACACAGAA GCTCGAACAT CTCTTTGAGT CCCGGGCCAA AGACTTGATG 
ACCAAGAAAC AACAAGAGCT GAACAAGAGC AAAGAGATCA TCGTGCTTGA TCACAAGCGC 
TCCAATGCCA TCAATATTGC GATCACTAAG CTGCCTCCTC CGAGGGCCAT TAAGACGGCC 
ATCCTCAAAA TGGATGCCAC TGTGGTCACA CGGGAGGGAA TCGACAAGCT ACTCAATATG 
CTGCCCACTG ATGAGGAGCG TGGCAAGATC CAGGAGGCCC AGTTATCCAA CCCAGAGTTG 
CCCCTGGGCA GCGCCGAGCA GTTCCTTTTG ACCCTGGCCT CCATTTCGGA GCTGGAAGCT 
CGCCTGAAAT TGTGGGCTTT CCGTCTGGAC TTTGACAACA GTGAGAAAGA GATTGCGGAA 
CCGCTGATGG ACCTCAAGCA AGGCATTGAG ATCCTGCGTC AGAATCGCAC CTTCCGCAGC 
ATCCTCTCTA CTCTGCTGTC TGTGGGCATC TTTTTGAATG GAGCTCCCGT CAAGGGATTC 
CAGATCGAGT ATCTGGCAAA GGTTCCGGAA GTGAAGGACA CGGTGCACAA GCACTCGCTG 
CTGCACCACC TCTGCCACAT GGTCATGGAG TCGAGCAGTG ATACTAGTGA TCTGTACTCC 
GAGATCGGAC CCATTACGCG AGCCTCCAAG GCGGATTTCA CGGACTTGGC ACACAATCTC 
AATCAGTTGG AGGCGGAGTG CAAGGCATGC TGGGATCGTC TGAAACTTAT TGCCAAACAC 
GACTGCCCGC CACCGTTAAA GCAGAAGCTG GTGGATTTCT TGGCCGATTG TGCGGAGCGC 
ATCATCATCC TGCAAATTGT CCATCGACGG GTGATGAATC GCTACCGGAA GTTCCTTTTG 
TGGCTGGGAA TGCCGCAGCA CAGTGTCGCG GAATCGCGTC CCAACGAATT CTGCCGGACA 
CTCTCCGAGT TCGCCCTGGA ATACCGCACC ACTAGAGAGC GGGTTCAGCA GCAGCTGGAG 
AAGAAGGCCA ACCACCGGGA ACGGAACAAG ACCCGCGGCA AACTGATCAT CGATATGGCC 
AAGTTCAAGA CCAAAGATGA CGTGGCCGAT GACGAGTTGA AAAAACTTTT GGACACATCG 
AGTGCCGACC AGGCCGATGG GACACTCACC TGGCGGAGGC GACGAGCGGA ACAGCTCCGC 
TCACCAATTA CCCGCCAAAG CGAGGAGCAG TTCACCGATG GCGACGATGA GATACTGGAA 
TCTCTGGTTA AGACCGCCAC CAAGGCTCCA GGAACACGGA CAACGCCGAG GGAACGGAAA 
CGGACGCGCC ACGCGGATCG CAAATCATTA CGTCGCACGC TGAAAAACGG TCTGACCGAG 
GAGGAGAAAC AGCACGTGGC TGCCTTGATA CAAACCTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729410.1
CDS	321..4502
Translation	MIVKMEPDELITFRVQYLVDSDPLSACTAFPIPVRAPTYAFATTMPLANQLGTILRLLNAPQSIDDAAIQVYKDGDYGAYLDLESSLAEQAEEIEGVNVSRKNSLVVRTQLNVRVQAIIEKLLSAQGSDLRRALFSLKQVFQEDKDLVHAFVALGGLNCLVRVGNCADQNYQNYILRALGQVMLYVDGMNGVMKHEPTMQWLYSLIASNYRSVVKTALKLLLVFVEYAESNCYVLVSAIHAVDASQGTLPWSNIMRLLKDYDNADAELVIYATSLINKTLAGLNDQDSFYDESDLLEQQGMETVIQRYMSKPGTDLDLLDQLQLYEAVLKFEDGESDGQRLPQNSMRKTQRYRPGANTTERRKSRRHSTDNSPASLTKVLPTTVLRMTPTQATVDEDSSGSTNSTEFSGGLFNEKKPRDGAGVTPGLRRRRERAERHKSFLKEQQEAAANGIFAALEQREGLGQIVTAIPATIQTGDSPPQSLQHEVPPCNNISGITNISNNNVSNLSCNDTPNNSLLVMSPSKQLLTGSHSISIINNSFDKASNNNQSEFLTKKNLFRERNSTVINGIMKNIQQTDSAYKKYENEANRLNNYYSQSPKHQIHQPIQPTPEVLLSPKKTLNASGFDFTPAPLSSTPVKCDVPPEPEPLSPVKKPAAAPPPPPPPPPPPPPPPGWWHAPIYNSPQARTLTSTNPELLPHPVDPSDMESEDQQLLLQLKRDHTVKDLTQKLSNLPMSPTQDSANRALVGDMSGLISKAKEGLAKSKSKGEISRSSSVDQDVKKSEPKKSENELHWEELVRNMTRPLNLCDLDFTDLRDDDEKDVLAPRSLGAGIPPPPPPLGGAIAPPPMMPPSLAPPPMYGYGGSLTNSVNSLNGSINGELVNGNNTIKKNKKTVKLFWKEVREDMIPQVVGKTIWDELPDANVDTQKLEHLFESRAKDLMTKKQQELNKSKEIIVLDHKRSNAINIAITKLPPPRAIKTAILKMDATVVTREGIDKLLNMLPTDEERGKIQEAQLSNPELPLGSAEQFLLTLASISELEARLKLWAFRLDFDNSEKEIAEPLMDLKQGIEILRQNRTFRSILSTLLSVGIFLNGAPVKGFQIEYLAKVPEVKDTVHKHSLLHHLCHMVMESSSDTSDLYSEIGPITRASKADFTDLAHNLNQLEAECKACWDRLKLIAKHDCPPPLKQKLVDFLADCAERIIILQIVHRRVMNRYRKFLLWLGMPQHSVAESRPNEFCRTLSEFALEYRTTRERVQQQLEKKANHRERNKTRGKLIIDMAKFKTKDDVADDELKKLLDTSSADQADGTLTWRRRRAEQLRSPITRQSEEQFTDGDDEILESLVKTATKAPGTRTTPRERKRTRHADRKSLRRTLKNGLTEEEKQHVAALIQTY

Target ORF information:

RefSeq Version	NM_168293.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster formin homology 2 domain containing, transcript variant A (Fhos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168293.3

ORF Insert Sequence:

ATGATTGTGA AAATGGAGCC GGACGAACTG ATCACATTCC GTGTCCAGTA TCTGGTGGAC 
AGCGATCCGC TCTCGGCGTG CACCGCCTTC CCGATCCCGG TGCGGGCGCC CACCTACGCC 
TTCGCCACCA CCATGCCGCT GGCCAATCAG CTGGGCACAA TCCTGCGGCT GCTGAATGCA 
CCACAAAGCA TCGACGATGC AGCCATTCAG GTGTACAAGG ATGGCGACTA CGGAGCCTAC 
TTGGATCTGG AGTCATCGCT GGCCGAACAG GCCGAGGAAA TCGAGGGAGT CAACGTCAGC 
CGCAAAAACT CTCTGGTGGT GCGAACGCAG TTAAATGTGC GCGTACAAGC CATAATTGAG 
AAACTGCTCT CCGCACAGGG CAGTGACCTG CGGCGTGCTC TCTTCTCGCT GAAGCAGGTG 
TTCCAGGAGG ATAAGGATCT GGTGCACGCC TTCGTGGCCC TCGGCGGCCT CAATTGCCTG 
GTGCGCGTGG GCAATTGTGC GGATCAAAAC TATCAGAATT ACATTCTGCG AGCCCTCGGT 
CAGGTCATGC TCTATGTGGA CGGAATGAAC GGCGTGATGA AGCATGAGCC GACGATGCAG 
TGGCTCTACT CGCTGATTGC CTCCAATTAC CGATCCGTGG TGAAGACCGC CCTCAAGTTG 
TTGCTGGTCT TCGTGGAGTA CGCCGAGTCC AACTGCTATG TGCTGGTCAG TGCCATTCAC 
GCAGTGGATG CTTCCCAGGG CACACTGCCC TGGTCAAATA TAATGAGACT GCTTAAGGAC 
TATGACAATG CTGATGCCGA ACTGGTGATC TATGCCACTT CGCTGATTAA CAAAACTCTG 
GCGGGTCTGA ATGATCAGGA CAGCTTCTAC GACGAAAGCG ATCTTCTAGA GCAGCAGGGC 
ATGGAGACTG TGATCCAGCG TTATATGTCC AAGCCGGGCA CTGATTTGGA TCTGCTCGAT 
CAGTTGCAAC TGTATGAGGC GGTTCTCAAA TTTGAGGATG GTGAGTCGGA TGGACAGCGT 
TTGCCGCAGA ACTCAATGCG AAAAACACAA CGCTATCGTC CGGGAGCAAA TACCACTGAA 
AGACGCAAGT CGCGTCGCCA TAGCACAGAT AATAGTCCCG CATCACTCAC AAAGGTCCTG 
CCCACCACTG TCCTCCGGAT GACACCTACA CAAGCTACGG TGGATGAGGA TAGTTCTGGC 
TCCACCAACT CAACGGAATT TAGTGGCGGT CTCTTTAATG AGAAGAAACC TCGTGATGGT 
GCAGGCGTGA CACCAGGACT TCGAAGAAGA CGTGAGCGGG CGGAGCGGCA TAAGAGCTTT 
CTGAAGGAGC AACAGGAGGC GGCGGCCAAT GGAATCTTTG CTGCTCTCGA GCAGCGGGAG 
GGACTGGGCC AGATTGTAAC CGCCATACCC GCTACCATTC AAACCGGCGA TAGTCCGCCT 
CAGTCCTTGC AACATGAGGT GCCACCGTGC AACAACATCA GCGGAATCAC CAACATTAGT 
AACAACAATG TCAGCAACCT TAGCTGCAAT GACACGCCGA ACAACAGCCT CTTGGTGATG 
AGTCCTAGCA AGCAACTGCT CACTGGAAGC CACAGCATCT CCATTATTAA CAACAGCTTC 
GATAAGGCGA GCAATAACAA TCAGAGCGAG TTCCTGACCA AGAAGAACCT ATTCCGGGAA 
CGCAATTCAA CTGTGATCAA TGGTATTATG AAGAATATCC AGCAAACCGA TAGTGCCTAT 
AAGAAGTACG AGAACGAAGC GAACCGGCTG AACAACTATT ACAGTCAGTC GCCGAAACAT 
CAGATCCATC AGCCCATTCA GCCAACGCCG GAAGTCCTTT TGAGTCCCAA AAAAACGCTA 
AATGCCTCTG GGTTTGATTT CACGCCCGCT CCCCTCAGTT CCACCCCGGT TAAGTGTGAT 
GTCCCGCCGG AACCGGAGCC ACTGAGTCCG GTAAAGAAAC CCGCTGCGGC ACCACCACCG 
CCTCCTCCAC CGCCACCGCC GCCACCACCA CCACCTGGTT GGTGGCACGC CCCCATCTAC 
AACAGCCCGC AAGCTCGAAC CCTAACCAGT ACTAACCCAG AGCTGCTGCC GCATCCTGTG 
GATCCTTCAG ATATGGAAAG CGAGGATCAG CAGCTGCTCC TCCAGCTGAA GCGGGACCAC 
ACGGTCAAGG ATCTCACCCA GAAGTTGAGC AACCTGCCAA TGAGTCCCAC TCAGGATTCG 
GCGAATCGCG CCCTCGTCGG CGATATGTCC GGTCTGATTT CGAAAGCCAA AGAGGGTCTG 
GCCAAGAGCA AGAGCAAGGG AGAGATATCC AGATCTTCTA GTGTCGATCA GGATGTAAAG 
AAGTCTGAGC CCAAGAAGTC GGAAAATGAA CTTCACTGGG AGGAACTGGT TAGGAATATG 
ACAAGGCCCC TAAATCTTTG CGATCTGGAC TTTACGGATC TGAGGGATGA TGACGAGAAG 
GATGTGCTGG CCCCTCGAAG TTTGGGTGCC GGTATACCCC CGCCGCCTCC CCCGCTGGGT 
GGAGCCATTG CCCCACCGCC AATGATGCCT CCAAGCTTGG CACCACCACC CATGTACGGA 
TATGGCGGTA GTCTAACCAA TAGTGTCAAT TCGTTAAATG GTTCTATAAA TGGTGAACTG 
GTCAATGGTA ATAACACCAT CAAAAAGAAC AAGAAAACGG TCAAGCTCTT CTGGAAGGAA 
GTGCGCGAGG ACATGATACC GCAGGTGGTG GGCAAGACCA TATGGGATGA GCTGCCCGAT 
GCCAACGTGG ACACACAGAA GCTCGAACAT CTCTTTGAGT CCCGGGCCAA AGACTTGATG 
ACCAAGAAAC AACAAGAGCT GAACAAGAGC AAAGAGATCA TCGTGCTTGA TCACAAGCGC 
TCCAATGCCA TCAATATTGC GATCACTAAG CTGCCTCCTC CGAGGGCCAT TAAGACGGCC 
ATCCTCAAAA TGGATGCCAC TGTGGTCACA CGGGAGGGAA TCGACAAGCT ACTCAATATG 
CTGCCCACTG ATGAGGAGCG TGGCAAGATC CAGGAGGCCC AGTTATCCAA CCCAGAGTTG 
CCCCTGGGCA GCGCCGAGCA GTTCCTTTTG ACCCTGGCCT CCATTTCGGA GCTGGAAGCT 
CGCCTGAAAT TGTGGGCTTT CCGTCTGGAC TTTGACAACA GTGAGAAAGA GATTGCGGAA 
CCGCTGATGG ACCTCAAGCA AGGCATTGAG ATCCTGCGTC AGAATCGCAC CTTCCGCAGC 
ATCCTCTCTA CTCTGCTGTC TGTGGGCATC TTTTTGAATG GAGCTCCCGT CAAGGGATTC 
CAGATCGAGT ATCTGGCAAA GGTTCCGGAA GTGAAGGACA CGGTGCACAA GCACTCGCTG 
CTGCACCACC TCTGCCACAT GGTCATGGAG TCGAGCAGTG ATACTAGTGA TCTGTACTCC 
GAGATCGGAC CCATTACGCG AGCCTCCAAG GCGGATTTCA CGGACTTGGC ACACAATCTC 
AATCAGTTGG AGGCGGAGTG CAAGGCATGC TGGGATCGTC TGAAACTTAT TGCCAAACAC 
GACTGCCCGC CACCGTTAAA GCAGAAGCTG GTGGATTTCT TGGCCGATTG TGCGGAGCGC 
ATCATCATCC TGCAAATTGT CCATCGACGG GTGATGAATC GCTACCGGAA GTTCCTTTTG 
TGGCTGGGAA TGCCGCAGCA CAGTGTCGCG GAATCGCGTC CCAACGAATT CTGCCGGACA 
CTCTCCGAGT TCGCCCTGGA ATACCGCACC ACTAGAGAGC GGGTTCAGCA GCAGCTGGAG 
AAGAAGGCCA ACCACCGGGA ACGGAACAAG ACCCGCGGCA AACTGATCAT CGATATGGCC 
AAGTTCAAGA CCAAAGATGA CGTGGCCGAT GACGAGTTGA AAAAACTTTT GGACACATCG 
AGTGCCGACC AGGCCGATGG GACACTCACC TGGCGGAGGC GACGAGCGGA ACAGCTCCGC 
TCACCAATTA CCCGCCAAAG CGAGGAGCAG TTCACCGATG GCGACGATGA GATACTGGAA 
TCTCTGGTTA AGACCGCCAC CAAGGCTCCA GGAACACGGA CAACGCCGAG GGAACGGAAA 
CGGACGCGCC ACGCGGATCG CAAATCATTA CGTCGCACGC TGAAAAACGG TCTGACCGAG 
GAGGAGAAAC AGCACGTGGC TGCCTTGATA CAAACCTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhos ( NM_001259755.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246684.1 Drosophila melanogaster formin homology 2 domain containing, transcript variant H (Fhos), mRNA.

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CloneID	OFa20758
Clone ID Related Accession (Same CDS sequence)	NM_001259755.1
Accession Version	NM_001259755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	formin homology 2 domain containing, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCTCTT ACAGGCTGAG ACACATTGAC TCTGGGGAGG AGCAGGCCTG GTGGCTACGC 
CAGGATGAGA ATGGCGATGA TGATGACACA CTGGCGGAGG AGACCCTCAA TACTCTAGAT 
GATGATCTCC AAGAGCCCAG CAAAAGCACC TGGTGGAGTC AGGACGATCA GGACTCTAGC 
AGTCAGGTGA AACTAATGCC CAGGAGTAGG ATAACGCCAG AAAAGGATGC CTGGTGGCTG 
CAGGACGAGG ATGAGGCTGC TAAATCTCCC ATAGAGAATG GTGATCTGAA CAACAACAAT 
AATAGCGAGC CTCATGTGGA GGTGACTCTA AGGTTACCAT CGAATGGCAG CCAAAGGAGC 
TCCAAAAGTA GTGCTTCCAA GAATGGAAGT AATCCGAAGA GTGGTGGCGC CAATCCTTGG 
TGGCTATCCG GACCAGCCAG AAAGATGTTC AATGTCCAAC GCGTTGAGTC GGGCGAAAGA 
GCGTGGTGGC AAGAGGAAGC CGAGGAGCCA CCGCAAGAGG AAAAAGAGAG CGAACCACCA 
CCGGTGAAGC CGCCGACGCC GCCACCGCGC ATCATCAAGC ACTGGGAGTC CGGAGAGCGG 
GCCTGGTGGT TACAGGATGA GACAGAAAGC AACCAACTCC CTGTTCAGGC TAGCAGCGAT 
GATGAGGAGC AACCAGTTTT GGTGGTGATT AATCAGCAGG AGTCTAGGGA TGAAGTGGAC 
CTGAGGCTGG CACCACCCAC TTTGGAATCC CAGGAGCTGA GCAACTCCTT CAATTTTGAC 
GCCCATTCCG AGCGACCTCC TCCACTGGGT CAATGTGCCA GTCCCGTTCG CTGTCCCTCG 
CCCTACGACA ACATTCCAAG ATCGCAACTG CAGGAGCCAC CACCACCACC CACTACCACG 
CTGACATCCC TGCAGCCGCG CTTTTCACCC CAAACCCGGC GAGCCGGCGA AAAGCTATTT 
ATTTCGAGGC ATCAAAACAT CGATGAGCTG CTGGGCGGAG CATGCCGTCC CCTCAGCCCG 
CTCTTCTATG GAAACAGCAA TCCTGCTGAG TCCGAGCTGA CTCCTCTAGC CGGAAAGAAC 
ATGTTCCTGC TTGAGGAGGT AACACCTGAT CAGGTGCGAA TTCACGACAG CACTGCCCAG 
CTGCCGGTTA TCCAGAGAAT GCAGCGCGAT GTCGAGTGGT TGGATAATGG CAGCAACTTT 
TATGAGCCAC CCGAACAAAG ACAACACCAG CCGCCGCGCA GGATCGACGA TGCAGCCATT 
CAGGTGTACA AGGATGGCGA CTACGGAGCC TACTTGGATC TGGAGTCATC GCTGGCCGAA 
CAGGCCGAGG AAATCGAGGG AGTCAACGTC AGCCGCAAAA ACTCTCTGGT GGTGCGAACG 
CAGTTAAATG TGCGCGTACA AGCCATAATT GAGAAACTGC TCTCCGCACA GGGCAGTGAC 
CTGCGGCGTG CTCTCTTCTC GCTGAAGCAG GTGTTCCAGG AGGATAAGGA TCTGGTGCAC 
GCCTTCGTGG CCCTCGGCGG CCTCAATTGC CTGGTGCGCG TGGGCAATTG TGCGGATCAA 
AACTATCAGA ATTACATTCT GCGAGCCCTC GGTCAGGTCA TGCTCTATGT GGACGGAATG 
AACGGCGTGA TGAAGCATGA GCCGACGATG CAGTGGCTCT ACTCGCTGAT TGCCTCCAAT 
TACCGATCCG TGGTGAAGAC CGCCCTCAAG TTGTTGCTGG TCTTCGTGGA GTACGCCGAG 
TCCAACTGCT ATGTGCTGGT CAGTGCCATT CACGCAGTGG ATGCTTCCCA GGGCACACTG 
CCCTGGTCAA ATATAATGAG ACTGCTTAAG GACTATGACA ATGCTGATGC CGAACTGGTG 
ATCTATGCCA CTTCGCTGAT TAACAAAACT CTGGCGGGTC TGAATGATCA GGACAGCTTC 
TACGACGAAA GCGATCTTCT AGAGCAGCAG GGCATGGAGA CTGTGATCCA GCGTTATATG 
TCCAAGCCGG GCACTGATTT GGATCTGCTC GATCAGTTGC AACTGTATGA GGCGGTTCTC 
AAATTTGAGG ATGGTGAGTC GGATGGACAG CGTTTGCCGC AGAACTCAAT GCGAAAAACA 
CAACGCTATC GTCCGGGAGC AAATACCACT GAAAGACGCA AGTCGCGTCG CCATAGCACA 
GATAATAGTC CCGCATCACT CACAAAGGTC CTGCCCACCA CTGTCCTCCG GATGACACCT 
ACACAAGCTA CGGTGGATGA GGATAGTTCT GGCTCCACCA ACTCAACGGA ATTTAGTGGC 
GGTCTCTTTA ATGAGAAGAA ACCTCGTGAT GGTGCAGGCG TGACACCAGG ACTTCGAAGA 
AGACGTGAGC GGGCGGAGCG GCATAAGAGC TTTCTGAAGG AGCAACAGGA GGCGGCGGCC 
AATGGAATCT TTGCTGCTCT CGAGCAGCGG GAGGGACTGG GCCAGATTGT AACCGCCATA 
CCCGCTACCA TTCAAACCGG CGATAGTCCG CCTCAGTCCT TGCAACATGA GGTGCCACCG 
TGCAACAACA TCAGCGGAAT CACCAACATT AGTAACAACA ATGTCAGCAA CCTTAGCTGC 
AATGACACGC CGAACAACAG CCTCTTGGTG ATGAGTCCTA GCAAGCAACT GCTCACTGGA 
AGCCACAGCA TCTCCATTAT TAACAACAGC TTCGATAAGG CGAGCAATAA CAATCAGAGC 
GAGTTCCTGA CCAAGAAGAA CCTATTCCGG GAACGCAATT CAACTGTGAT CAATGGTATT 
ATGAAGAATA TCCAGCAAAC CGATAGTGCC TATAAGAAGT ACGAGAACGA AGCGAACCGG 
CTGAACAACT ATTACAGTCA GTCGCCGAAA CATCAGATCC ATCAGCCCAT TCAGCCAACG 
CCGGAAGTCC TTTTGAGTCC CAAAAAAACG CTAAATGCCT CTGGGTTTGA TTTCACGCCC 
GCTCCCCTCA GTTCCACCCC GGTTAAGTGT GATGTCCCGC CGGAACCGGA GCCACTGAGT 
CCGGTAAAGA AACCCGCTGC GGCACCACCA CCGCCTCCTC CACCGCCACC GCCGCCACCA 
CCACCACCTG GTTGGTGGCA CGCCCCCATC TACAACAGCC CGCAAGCTCG AACCCTAACC 
AGTACTAACC CAGAGCTGCT GCCGCATCCT GTGGATCCTT CAGATATGGA AAGCGAGGAT 
CAGCAGCTGC TCCTCCAGCT GAAGCGGGAC CACACGGTCA AGGATCTCAC CCAGAAGTTG 
AGCAACCTGC CAATGAGTCC CACTCAGGAT TCGGCGAATC GCGCCCTCGT CGGCGATATG 
TCCGGTCTGA TTTCGAAAGC CAAAGAGGGT CTGGCCAAGA GCAAGAGCAA GGGAGAGATA 
TCCAGATCTT CTAGTGTCGA TCAGGATGTA AAGAAGTCTG AGCCCAAGAA GTCGGAAAAT 
GAACTTCACT GGGAGGAACT GGTTAGGAAT ATGACAAGGC CCCTAAATCT TTGCGATCTG 
GACTTTACGG ATCTGAGGGA TGATGACGAG AAGGATGTGC TGGCCCCTCG AAGTTTGGGT 
GCCGGTATAC CCCCGCCGCC TCCCCCGCTG GGTGGAGCCA TTGCCCCACC GCCAATGATG 
CCTCCAAGCT TGGCACCACC ACCCATGTAC GGATATGGCG GTAGTCTAAC CAATAGTGTC 
AATTCGTTAA ATGGTTCTAT AAATGGTGAA CTGGTCAATG GTAATAACAC CATCAAAAAG 
AACAAGAAAA CGGTCAAGCT CTTCTGGAAG GAAGTGCGCG AGGACATGAT ACCGCAGGTG 
GTGGGCAAGA CCATATGGGA TGAGCTGCCC GATGCCAACG TGGACACACA GAAGCTCGAA 
CATCTCTTTG AGTCCCGGGC CAAAGACTTG ATGACCAAGA AACAACAAGA GCTGAACAAG 
AGCAAAGAGA TCATCGTGCT TGATCACAAG CGCTCCAATG CCATCAATAT TGCGATCACT 
AAGCTGCCTC CTCCGAGGGC CATTAAGACG GCCATCCTCA AAATGGATGC CACTGTGGTC 
ACACGGGAGG GAATCGACAA GCTACTCAAT ATGCTGCCCA CTGATGAGGA GCGTGGCAAG 
ATCCAGGAGG CCCAGTTATC CAACCCAGAG TTGCCCCTGG GCAGCGCCGA GCAGTTCCTT 
TTGACCCTGG CCTCCATTTC GGAGCTGGAA GCTCGCCTGA AATTGTGGGC TTTCCGTCTG 
GACTTTGACA ACAGTGAGAA AGAGATTGCG GAACCGCTGA TGGACCTCAA GCAAGGCATT 
GAGATCCTGC GTCAGAATCG CACCTTCCGC AGCATCCTCT CTACTCTGCT GTCTGTGGGC 
ATCTTTTTGA ATGGAGCTCC CGTCAAGGGA TTCCAGATCG AGTATCTGGC AAAGGTTCCG 
GAAGTGAAGG ACACGGTGCA CAAGCACTCG CTGCTGCACC ACCTCTGCCA CATGGTCATG 
GAGTCGAGCA GTGATACTAG TGATCTGTAC TCCGAGATCG GACCCATTAC GCGAGCCTCC 
AAGGCGGATT TCACGGACTT GGCACACAAT CTCAATCAGT TGGAGGCGGA GTGCAAGGCA 
TGCTGGGATC GTCTGAAACT TATTGCCAAA CACGACTGCC CGCCACCGTT AAAGCAGAAG 
CTGGTGGATT TCTTGGCCGA TTGTGCGGAG CGCATCATCA TCCTGCAAAT TGTCCATCGA 
CGGGTGATGA ATCGCTACCG GAAGTTCCTT TTGTGGCTGG GAATGCCGCA GCACAGTGTC 
GCGGAATCGC GTCCCAACGA ATTCTGCCGG ACACTCTCCG AGTTCGCCCT GGAATACCGC 
ACCACTAGAG AGCGGGTTCA GCAGCAGCTG GAGAAGAAGG CCAACCACCG GGAACGGAAC 
AAGACCCGCG GCAAACTGAT CATCGATATG GCCAAGTTCA AGACCAAAGA TGACGTGGCC 
GATGACGAGT TGAAAAAACT TTTGGACACA TCGAGTGCCG ACCAGGCCGA TGGGACACTC 
ACCTGGCGGA GGCGACGAGC GGAACAGCTC CGCTCACCAA TTACCCGCCA AAGCGAGGAG 
CAGTTCACCG ATGGCGACGA TGAGATACTG GAATCTCTGG TTAAGACCGC CACCAAGGCT 
CCAGGAACAC GGACAACGCC GAGGGAACGG AAACGGACGC GCCACGCGGA TCGCAAATCA 
TTACGTCGCA CGCTGAAAAA CGGTCTGACC GAGGAGGAGA AACAGCACGT GGCTGCCTTG 
ATACAAACCT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246684.1
CDS	270..5504
Translation	MPSYRLRHIDSGEEQAWWLRQDENGDDDDTLAEETLNTLDDDLQEPSKSTWWSQDDQDSSSQVKLMPRSRITPEKDAWWLQDEDEAAKSPIENGDLNNNNNSEPHVEVTLRLPSNGSQRSSKSSASKNGSNPKSGGANPWWLSGPARKMFNVQRVESGERAWWQEEAEEPPQEEKESEPPPVKPPTPPPRIIKHWESGERAWWLQDETESNQLPVQASSDDEEQPVLVVINQQESRDEVDLRLAPPTLESQELSNSFNFDAHSERPPPLGQCASPVRCPSPYDNIPRSQLQEPPPPPTTTLTSLQPRFSPQTRRAGEKLFISRHQNIDELLGGACRPLSPLFYGNSNPAESELTPLAGKNMFLLEEVTPDQVRIHDSTAQLPVIQRMQRDVEWLDNGSNFYEPPEQRQHQPPRRIDDAAIQVYKDGDYGAYLDLESSLAEQAEEIEGVNVSRKNSLVVRTQLNVRVQAIIEKLLSAQGSDLRRALFSLKQVFQEDKDLVHAFVALGGLNCLVRVGNCADQNYQNYILRALGQVMLYVDGMNGVMKHEPTMQWLYSLIASNYRSVVKTALKLLLVFVEYAESNCYVLVSAIHAVDASQGTLPWSNIMRLLKDYDNADAELVIYATSLINKTLAGLNDQDSFYDESDLLEQQGMETVIQRYMSKPGTDLDLLDQLQLYEAVLKFEDGESDGQRLPQNSMRKTQRYRPGANTTERRKSRRHSTDNSPASLTKVLPTTVLRMTPTQATVDEDSSGSTNSTEFSGGLFNEKKPRDGAGVTPGLRRRRERAERHKSFLKEQQEAAANGIFAALEQREGLGQIVTAIPATIQTGDSPPQSLQHEVPPCNNISGITNISNNNVSNLSCNDTPNNSLLVMSPSKQLLTGSHSISIINNSFDKASNNNQSEFLTKKNLFRERNSTVINGIMKNIQQTDSAYKKYENEANRLNNYYSQSPKHQIHQPIQPTPEVLLSPKKTLNASGFDFTPAPLSSTPVKCDVPPEPEPLSPVKKPAAAPPPPPPPPPPPPPPPGWWHAPIYNSPQARTLTSTNPELLPHPVDPSDMESEDQQLLLQLKRDHTVKDLTQKLSNLPMSPTQDSANRALVGDMSGLISKAKEGLAKSKSKGEISRSSSVDQDVKKSEPKKSENELHWEELVRNMTRPLNLCDLDFTDLRDDDEKDVLAPRSLGAGIPPPPPPLGGAIAPPPMMPPSLAPPPMYGYGGSLTNSVNSLNGSINGELVNGNNTIKKNKKTVKLFWKEVREDMIPQVVGKTIWDELPDANVDTQKLEHLFESRAKDLMTKKQQELNKSKEIIVLDHKRSNAINIAITKLPPPRAIKTAILKMDATVVTREGIDKLLNMLPTDEERGKIQEAQLSNPELPLGSAEQFLLTLASISELEARLKLWAFRLDFDNSEKEIAEPLMDLKQGIEILRQNRTFRSILSTLLSVGIFLNGAPVKGFQIEYLAKVPEVKDTVHKHSLLHHLCHMVMESSSDTSDLYSEIGPITRASKADFTDLAHNLNQLEAECKACWDRLKLIAKHDCPPPLKQKLVDFLADCAERIIILQIVHRRVMNRYRKFLLWLGMPQHSVAESRPNEFCRTLSEFALEYRTTRERVQQQLEKKANHRERNKTRGKLIIDMAKFKTKDDVADDELKKLLDTSSADQADGTLTWRRRRAEQLRSPITRQSEEQFTDGDDEILESLVKTATKAPGTRTTPRERKRTRHADRKSLRRTLKNGLTEEEKQHVAALIQTY

Target ORF information:

RefSeq Version	NM_001259755.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster formin homology 2 domain containing, transcript variant H (Fhos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259755.1

ORF Insert Sequence:

ATGCCCTCTT ACAGGCTGAG ACACATTGAC TCTGGGGAGG AGCAGGCCTG GTGGCTACGC 
CAGGATGAGA ATGGCGATGA TGATGACACA CTGGCGGAGG AGACCCTCAA TACTCTAGAT 
GATGATCTCC AAGAGCCCAG CAAAAGCACC TGGTGGAGTC AGGACGATCA GGACTCTAGC 
AGTCAGGTGA AACTAATGCC CAGGAGTAGG ATAACGCCAG AAAAGGATGC CTGGTGGCTG 
CAGGACGAGG ATGAGGCTGC TAAATCTCCC ATAGAGAATG GTGATCTGAA CAACAACAAT 
AATAGCGAGC CTCATGTGGA GGTGACTCTA AGGTTACCAT CGAATGGCAG CCAAAGGAGC 
TCCAAAAGTA GTGCTTCCAA GAATGGAAGT AATCCGAAGA GTGGTGGCGC CAATCCTTGG 
TGGCTATCCG GACCAGCCAG AAAGATGTTC AATGTCCAAC GCGTTGAGTC GGGCGAAAGA 
GCGTGGTGGC AAGAGGAAGC CGAGGAGCCA CCGCAAGAGG AAAAAGAGAG CGAACCACCA 
CCGGTGAAGC CGCCGACGCC GCCACCGCGC ATCATCAAGC ACTGGGAGTC CGGAGAGCGG 
GCCTGGTGGT TACAGGATGA GACAGAAAGC AACCAACTCC CTGTTCAGGC TAGCAGCGAT 
GATGAGGAGC AACCAGTTTT GGTGGTGATT AATCAGCAGG AGTCTAGGGA TGAAGTGGAC 
CTGAGGCTGG CACCACCCAC TTTGGAATCC CAGGAGCTGA GCAACTCCTT CAATTTTGAC 
GCCCATTCCG AGCGACCTCC TCCACTGGGT CAATGTGCCA GTCCCGTTCG CTGTCCCTCG 
CCCTACGACA ACATTCCAAG ATCGCAACTG CAGGAGCCAC CACCACCACC CACTACCACG 
CTGACATCCC TGCAGCCGCG CTTTTCACCC CAAACCCGGC GAGCCGGCGA AAAGCTATTT 
ATTTCGAGGC ATCAAAACAT CGATGAGCTG CTGGGCGGAG CATGCCGTCC CCTCAGCCCG 
CTCTTCTATG GAAACAGCAA TCCTGCTGAG TCCGAGCTGA CTCCTCTAGC CGGAAAGAAC 
ATGTTCCTGC TTGAGGAGGT AACACCTGAT CAGGTGCGAA TTCACGACAG CACTGCCCAG 
CTGCCGGTTA TCCAGAGAAT GCAGCGCGAT GTCGAGTGGT TGGATAATGG CAGCAACTTT 
TATGAGCCAC CCGAACAAAG ACAACACCAG CCGCCGCGCA GGATCGACGA TGCAGCCATT 
CAGGTGTACA AGGATGGCGA CTACGGAGCC TACTTGGATC TGGAGTCATC GCTGGCCGAA 
CAGGCCGAGG AAATCGAGGG AGTCAACGTC AGCCGCAAAA ACTCTCTGGT GGTGCGAACG 
CAGTTAAATG TGCGCGTACA AGCCATAATT GAGAAACTGC TCTCCGCACA GGGCAGTGAC 
CTGCGGCGTG CTCTCTTCTC GCTGAAGCAG GTGTTCCAGG AGGATAAGGA TCTGGTGCAC 
GCCTTCGTGG CCCTCGGCGG CCTCAATTGC CTGGTGCGCG TGGGCAATTG TGCGGATCAA 
AACTATCAGA ATTACATTCT GCGAGCCCTC GGTCAGGTCA TGCTCTATGT GGACGGAATG 
AACGGCGTGA TGAAGCATGA GCCGACGATG CAGTGGCTCT ACTCGCTGAT TGCCTCCAAT 
TACCGATCCG TGGTGAAGAC CGCCCTCAAG TTGTTGCTGG TCTTCGTGGA GTACGCCGAG 
TCCAACTGCT ATGTGCTGGT CAGTGCCATT CACGCAGTGG ATGCTTCCCA GGGCACACTG 
CCCTGGTCAA ATATAATGAG ACTGCTTAAG GACTATGACA ATGCTGATGC CGAACTGGTG 
ATCTATGCCA CTTCGCTGAT TAACAAAACT CTGGCGGGTC TGAATGATCA GGACAGCTTC 
TACGACGAAA GCGATCTTCT AGAGCAGCAG GGCATGGAGA CTGTGATCCA GCGTTATATG 
TCCAAGCCGG GCACTGATTT GGATCTGCTC GATCAGTTGC AACTGTATGA GGCGGTTCTC 
AAATTTGAGG ATGGTGAGTC GGATGGACAG CGTTTGCCGC AGAACTCAAT GCGAAAAACA 
CAACGCTATC GTCCGGGAGC AAATACCACT GAAAGACGCA AGTCGCGTCG CCATAGCACA 
GATAATAGTC CCGCATCACT CACAAAGGTC CTGCCCACCA CTGTCCTCCG GATGACACCT 
ACACAAGCTA CGGTGGATGA GGATAGTTCT GGCTCCACCA ACTCAACGGA ATTTAGTGGC 
GGTCTCTTTA ATGAGAAGAA ACCTCGTGAT GGTGCAGGCG TGACACCAGG ACTTCGAAGA 
AGACGTGAGC GGGCGGAGCG GCATAAGAGC TTTCTGAAGG AGCAACAGGA GGCGGCGGCC 
AATGGAATCT TTGCTGCTCT CGAGCAGCGG GAGGGACTGG GCCAGATTGT AACCGCCATA 
CCCGCTACCA TTCAAACCGG CGATAGTCCG CCTCAGTCCT TGCAACATGA GGTGCCACCG 
TGCAACAACA TCAGCGGAAT CACCAACATT AGTAACAACA ATGTCAGCAA CCTTAGCTGC 
AATGACACGC CGAACAACAG CCTCTTGGTG ATGAGTCCTA GCAAGCAACT GCTCACTGGA 
AGCCACAGCA TCTCCATTAT TAACAACAGC TTCGATAAGG CGAGCAATAA CAATCAGAGC 
GAGTTCCTGA CCAAGAAGAA CCTATTCCGG GAACGCAATT CAACTGTGAT CAATGGTATT 
ATGAAGAATA TCCAGCAAAC CGATAGTGCC TATAAGAAGT ACGAGAACGA AGCGAACCGG 
CTGAACAACT ATTACAGTCA GTCGCCGAAA CATCAGATCC ATCAGCCCAT TCAGCCAACG 
CCGGAAGTCC TTTTGAGTCC CAAAAAAACG CTAAATGCCT CTGGGTTTGA TTTCACGCCC 
GCTCCCCTCA GTTCCACCCC GGTTAAGTGT GATGTCCCGC CGGAACCGGA GCCACTGAGT 
CCGGTAAAGA AACCCGCTGC GGCACCACCA CCGCCTCCTC CACCGCCACC GCCGCCACCA 
CCACCACCTG GTTGGTGGCA CGCCCCCATC TACAACAGCC CGCAAGCTCG AACCCTAACC 
AGTACTAACC CAGAGCTGCT GCCGCATCCT GTGGATCCTT CAGATATGGA AAGCGAGGAT 
CAGCAGCTGC TCCTCCAGCT GAAGCGGGAC CACACGGTCA AGGATCTCAC CCAGAAGTTG 
AGCAACCTGC CAATGAGTCC CACTCAGGAT TCGGCGAATC GCGCCCTCGT CGGCGATATG 
TCCGGTCTGA TTTCGAAAGC CAAAGAGGGT CTGGCCAAGA GCAAGAGCAA GGGAGAGATA 
TCCAGATCTT CTAGTGTCGA TCAGGATGTA AAGAAGTCTG AGCCCAAGAA GTCGGAAAAT 
GAACTTCACT GGGAGGAACT GGTTAGGAAT ATGACAAGGC CCCTAAATCT TTGCGATCTG 
GACTTTACGG ATCTGAGGGA TGATGACGAG AAGGATGTGC TGGCCCCTCG AAGTTTGGGT 
GCCGGTATAC CCCCGCCGCC TCCCCCGCTG GGTGGAGCCA TTGCCCCACC GCCAATGATG 
CCTCCAAGCT TGGCACCACC ACCCATGTAC GGATATGGCG GTAGTCTAAC CAATAGTGTC 
AATTCGTTAA ATGGTTCTAT AAATGGTGAA CTGGTCAATG GTAATAACAC CATCAAAAAG 
AACAAGAAAA CGGTCAAGCT CTTCTGGAAG GAAGTGCGCG AGGACATGAT ACCGCAGGTG 
GTGGGCAAGA CCATATGGGA TGAGCTGCCC GATGCCAACG TGGACACACA GAAGCTCGAA 
CATCTCTTTG AGTCCCGGGC CAAAGACTTG ATGACCAAGA AACAACAAGA GCTGAACAAG 
AGCAAAGAGA TCATCGTGCT TGATCACAAG CGCTCCAATG CCATCAATAT TGCGATCACT 
AAGCTGCCTC CTCCGAGGGC CATTAAGACG GCCATCCTCA AAATGGATGC CACTGTGGTC 
ACACGGGAGG GAATCGACAA GCTACTCAAT ATGCTGCCCA CTGATGAGGA GCGTGGCAAG 
ATCCAGGAGG CCCAGTTATC CAACCCAGAG TTGCCCCTGG GCAGCGCCGA GCAGTTCCTT 
TTGACCCTGG CCTCCATTTC GGAGCTGGAA GCTCGCCTGA AATTGTGGGC TTTCCGTCTG 
GACTTTGACA ACAGTGAGAA AGAGATTGCG GAACCGCTGA TGGACCTCAA GCAAGGCATT 
GAGATCCTGC GTCAGAATCG CACCTTCCGC AGCATCCTCT CTACTCTGCT GTCTGTGGGC 
ATCTTTTTGA ATGGAGCTCC CGTCAAGGGA TTCCAGATCG AGTATCTGGC AAAGGTTCCG 
GAAGTGAAGG ACACGGTGCA CAAGCACTCG CTGCTGCACC ACCTCTGCCA CATGGTCATG 
GAGTCGAGCA GTGATACTAG TGATCTGTAC TCCGAGATCG GACCCATTAC GCGAGCCTCC 
AAGGCGGATT TCACGGACTT GGCACACAAT CTCAATCAGT TGGAGGCGGA GTGCAAGGCA 
TGCTGGGATC GTCTGAAACT TATTGCCAAA CACGACTGCC CGCCACCGTT AAAGCAGAAG 
CTGGTGGATT TCTTGGCCGA TTGTGCGGAG CGCATCATCA TCCTGCAAAT TGTCCATCGA 
CGGGTGATGA ATCGCTACCG GAAGTTCCTT TTGTGGCTGG GAATGCCGCA GCACAGTGTC 
GCGGAATCGC GTCCCAACGA ATTCTGCCGG ACACTCTCCG AGTTCGCCCT GGAATACCGC 
ACCACTAGAG AGCGGGTTCA GCAGCAGCTG GAGAAGAAGG CCAACCACCG GGAACGGAAC 
AAGACCCGCG GCAAACTGAT CATCGATATG GCCAAGTTCA AGACCAAAGA TGACGTGGCC 
GATGACGAGT TGAAAAAACT TTTGGACACA TCGAGTGCCG ACCAGGCCGA TGGGACACTC 
ACCTGGCGGA GGCGACGAGC GGAACAGCTC CGCTCACCAA TTACCCGCCA AAGCGAGGAG 
CAGTTCACCG ATGGCGACGA TGAGATACTG GAATCTCTGG TTAAGACCGC CACCAAGGCT 
CCAGGAACAC GGACAACGCC GAGGGAACGG AAACGGACGC GCCACGCGGA TCGCAAATCA 
TTACGTCGCA CGCTGAAAAA CGGTCTGACC GAGGAGGAGA AACAGCACGT GGCTGCCTTG 
ATACAAACCT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhos ( NM_001202143.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189072.1 Drosophila melanogaster formin homology 2 domain containing, transcript variant D (Fhos), mRNA.

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CloneID	OFa20759
Clone ID Related Accession (Same CDS sequence)	NM_001202143.2
Accession Version	NM_001202143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	formin homology 2 domain containing, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202143.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGTGA AAATGGAGCC GGACGAACTG ATCACATTCC GTGTCCAGTA TCTGGTGGAC 
AGCGATCCGC TCTCGGCGTG CACCGCCTTC CCGATCCCGG TGCGGGCGCC CACCTACGCC 
TTCGCCACCA CCATGCCGCT GGCCAATCAG CTGGGCACAA TCCTGCGGCT GCTGAATGCA 
CCACAAAGCA TCGACGATGC AGCCATTCAG GTGTACAAGG ATGGCGACTA CGGAGCCTAC 
TTGGATCTGG AGTCATCGCT GGCCGAACAG GCCGAGGAAA TCGAGGGAGT CAACGTCAGC 
CGCAAAAACT CTCTGGTGGT GCGAACGCAG TTAAATGTGC GCGTACAAGC CATAATTGAG 
AAACTGCTCT CCGCACAGGG CAGTGACCTG CGGCGTGCTC TCTTCTCGCT GAAGCAGGTG 
TTCCAGGAGG ATAAGGATCT GGTGCACGCC TTCGTGGCCC TCGGCGGCCT CAATTGCCTG 
GTGCGCGTGG GCAATTGTGC GGATCAAAAC TATCAGAATT ACATTCTGCG AGCCCTCGGT 
CAGGTCATGC TCTATGTGGA CGGAATGAAC GGCGTGATGA AGCATGAGCC GACGATGCAG 
TGGCTCTACT CGCTGATTGC CTCCAATTAC CGATCCGTGG TGAAGACCGC CCTCAAGTTG 
TTGCTGGTCT TCGTGGAGTA CGCCGAGTCC AACTGCTATG TGCTGGTCAG TGCCATTCAC 
GCAGTGGATG CTTCCCAGGG CACACTGCCC TGGTCAAATA TAATGAGACT GCTTAAGGAC 
TATGACAATG CTGATGCCGA ACTGGTGATC TATGCCACTT CGCTGATTAA CAAAACTCTG 
GCGGGTCTGA ATGATCAGGA CAGCTTCTAC GACGAAAGCG ATCTTCTAGA GCAGCAGGGC 
ATGGAGACTG TGATCCAGCG TTATATGTCC AAGCCGGGCA CTGATTTGGA TCTGCTCGAT 
CAGTTGCAAC TGTATGAGGC GGTTCTCAAA TTTGAGGATG GTGAGTCGGA TGGACAGCGT 
TTGCCGCAGA ACTCAATGCG AAAAACACAA CGCTATCGTC CGGGAGCAAA TACCACTGAA 
AGACGCAAGT CGCGTCGCCA TAGCACAGAT AATAGTCCCG CATCACTCAC AAAGGTCCTG 
CCCACCACTG TCCTCCGGAT GACACCTACA CAAGCTACGG TGGATGAGGA TAGTTCTGGC 
TCCACCAACT CAACGGAATT TAGTGGCGGT CTCTTTAATG AGAAGAAACC TCGTGATGGT 
GCAGGCGTGA CACCAGGACT TCGAAGAAGA CGTGAGCGGG CGGAGCGGCA TAAGAGCTTT 
CTGAAGGAGC AACAGGAGGC GGCGGCCAAT GGAATCTTTG CTGCTCTCGA GCAGCGGGAG 
GGACTGGGCC AGATTGTAAC CGCCATACCC GCTACCATTC AAACCGGCGA TAGTCCGCCT 
CAGTCCTTGC AACATGAGGT GCCACCGTGC AACAACATCA GCGGAATCAC CAACATTAGT 
AACAACAATG TCAGCAACCT TAGCTGCAAT GACACGCCGA ACAACAGCCT CTTGGTGATG 
AGTCCTAGCA AGCAACTGCT CACTGGAAGC CACAGCATCT CCATTATTAA CAACAGCTTC 
GATAAGGCGA GCAATAACAA TCAGAGCGAG TTCCTGACCA AGAAGAACCT ATTCCGGGAA 
CGCAATTCAA CTGTGATCAA TGGTATTATG AAGAATATCC AGCAAACCGA TAGTGCCTAT 
AAGAAGTACG AGAACGAAGC GAACCGGCTG AACAACTATT ACAGTCAGTC GCCGAAACAT 
CAGATCCATC AGCCCATTCA GCCAACGCCG GAAGTCCTTT TGAGTCCCAA AAAAACGCTA 
AATGCCTCTG GGTTTGATTT CACGCCCGCT CCCCTCAGTT CCACCCCGGT TAAGTGTGAT 
GTCCCGCCGG AACCGGAGCC ACTGAGTCCG GTAAAGAAAC CCGCTGCGGC ACCACCACCG 
CCTCCTCCAC CGCCACCGCC GCCACCACCA CCACCTGGTT GGTGGCACGC CCCCATCTAC 
AACAGCCCGC AAGCTCGAAC CCTAACCAGT ACTAACCCAG AGCTGCTGCC GCATCCTGTG 
GATCCTTCAG ATATGGAAAG CGAGGATCAG CAGCTGCTCC TCCAGCTGAA GCGGGACCAC 
ACGGTCAAGG ATCTCACCCA GAAGTTGAGC AACCTGCCAA TGAGTCCCAC TCAGGATTCG 
GCGAATCGCG CCCTCGTCGG CGATATGTCC GGTCTGATTT CGAAAGCCAA AGAGGGTCTG 
GCCAAGAGCA AGAGCAAGGG AGAGATATCC AGATCTTCTA GTGTCGATCA GGATGTAAAG 
AAGTCTGAGC CCAAGAAGTC GGAAAATGAA CTTCACTGGG AGGAACTGGT TAGGAATATG 
ACAAGGCCCC TAAATCTTTG CGATCTGGAC TTTACGGATC TGAGGGATGA TGACGAGAAG 
GATGTGCTGG CCCCTCGAAG TTTGGGTGCC GGTATACCCC CGCCGCCTCC CCCGCTGGGT 
GGAGCCATTG CCCCACCGCC AATGATGCCT CCAAGCTTGG CACCACCACC CATGTACGGA 
TATGGCGGTA GTCTAACCAA TAGTGTCAAT TCGTTAAATG GTTCTATAAA TGGTGAACTG 
GTCAATGGTA ATAACACCAT CAAAAAGAAC AAGAAAACGG TCAAGCTCTT CTGGAAGGAA 
GTGCGCGAGG ACATGATACC GCAGGTGGTG GGCAAGACCA TATGGGATGA GCTGCCCGAT 
GCCAACGTGG ACACACAGAA GCTCGAACAT CTCTTTGAGT CCCGGGCCAA AGACTTGATG 
ACCAAGAAAC AACAAGAGCT GAACAAGAGC AAAGAGATCA TCGTGCTTGA TCACAAGCGC 
TCCAATGCCA TCAATATTGC GATCACTAAG CTGCCTCCTC CGAGGGCCAT TAAGACGGCC 
ATCCTCAAAA TGGATGCCAC TGTGGTCACA CGGGAGGGAA TCGACAAGCT ACTCAATATG 
CTGCCCACTG ATGAGGAGCG TGGCAAGATC CAGGAGGCCC AGTTATCCAA CCCAGAGTTG 
CCCCTGGGCA GCGCCGAGCA GTTCCTTTTG ACCCTGGCCT CCATTTCGGA GCTGGAAGCT 
CGCCTGAAAT TGTGGGCTTT CCGTCTGGAC TTTGACAACA GTGAGAAAGA GATTGCGGAA 
CCGCTGATGG ACCTCAAGCA AGGCATTGAG ATCCTGCGTC AGAATCGCAC CTTCCGCAGC 
ATCCTCTCTA CTCTGCTGTC TGTGGGCATC TTTTTGAATG GAGCTCCCGT CAAGGGATTC 
CAGATCGAGT ATCTGGCAAA GGTTCCGGAA GTGAAGGACA CGGTGCACAA GCACTCGCTG 
CTGCACCACC TCTGCCACAT GGTCATGGAG TCGAGCAGTG ATACTAGTGA TCTGTACTCC 
GAGATCGGAC CCATTACGCG AGCCTCCAAG GCGGATTTCA CGGACTTGGC ACACAATCTC 
AATCAGTTGG AGGCGGAGTG CAAGGCATGC TGGGATCGTC TGAAACTTAT TGCCAAACAC 
GACTGCCCGC CACCGTTAAA GCAGAAGCTG GTGGATTTCT TGGCCGATTG TGCGGAGCGC 
ATCATCATCC TGCAAATTGT CCATCGACGG GTGATGAATC GCTACCGGAA GTTCCTTTTG 
TGGCTGGGAA TGCCGCAGCA CAGTGTCGCG GAATCGCGTC CCAACGAATT CTGCCGGACA 
CTCTCCGAGT TCGCCCTGGA ATACCGCACC ACTAGAGAGC GGGTTCAGCA GCAGCTGGAG 
AAGAAGGCCA ACCACCGGGA ACGGAACAAG ACCCGCGGCA AACTGATCAT CGATATGGCC 
AAGTTCAAGA CCAAAGATGA CGTGGCCGAT GACGAGTTGA AAAAACTTTT GGACACATCG 
AGTGCCGACC AGGCCGATGG GACACTCACC TGGCGGAGGC GACGAGCGGA ACAGCTCCGC 
TCACCAATTA CCCGCCAAAG CGAGGAGCAG TTCACCGATG GCGACGATGA GATACTGGAA 
TCTCTGGTTA AGACCGCCAC CAAGGCTCCA GGAACACGGA CAACGCCGAG GGAACGGAAA 
CGGACGCGCC ACGCGGATCG CAAATCATGT AAGTTGACCG CACAAGTAAT ACGATTGAGA 
TCCACGTCCA AAACTCCGAT AGAGAGAATA CGTTTTAATG AAAAGGTGTT GGTCTATATC 
TTTTCGAATC GCACACAGCG CGCTATGTCT ATAACCTCTA GCTGGATACT TTCAACCAGA 
AACACACACT ACACCATCAA GAATTCCACA GAGAAACTAC TATATATAGT TATACAAATA 
CTTATTTCCC ACACAAAATT CCAAATTTAC ACAAGTACCT GCAGTTTCCC ATACTTATTT 
ACAATATTTT CCCGATTACG CAGCTTTTGT CCCCTTAGTT TTGCCAGCTT ACGAATCTGT 
TGCCTATATT GGGTTTTAAT TTATTTCCAT TTCGTTCTTT TACCATTAAC GCACCAATCC 
AACAAACCAA TAAAAAATCA AATAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189072.1
CDS	321..4850
Translation	MIVKMEPDELITFRVQYLVDSDPLSACTAFPIPVRAPTYAFATTMPLANQLGTILRLLNAPQSIDDAAIQVYKDGDYGAYLDLESSLAEQAEEIEGVNVSRKNSLVVRTQLNVRVQAIIEKLLSAQGSDLRRALFSLKQVFQEDKDLVHAFVALGGLNCLVRVGNCADQNYQNYILRALGQVMLYVDGMNGVMKHEPTMQWLYSLIASNYRSVVKTALKLLLVFVEYAESNCYVLVSAIHAVDASQGTLPWSNIMRLLKDYDNADAELVIYATSLINKTLAGLNDQDSFYDESDLLEQQGMETVIQRYMSKPGTDLDLLDQLQLYEAVLKFEDGESDGQRLPQNSMRKTQRYRPGANTTERRKSRRHSTDNSPASLTKVLPTTVLRMTPTQATVDEDSSGSTNSTEFSGGLFNEKKPRDGAGVTPGLRRRRERAERHKSFLKEQQEAAANGIFAALEQREGLGQIVTAIPATIQTGDSPPQSLQHEVPPCNNISGITNISNNNVSNLSCNDTPNNSLLVMSPSKQLLTGSHSISIINNSFDKASNNNQSEFLTKKNLFRERNSTVINGIMKNIQQTDSAYKKYENEANRLNNYYSQSPKHQIHQPIQPTPEVLLSPKKTLNASGFDFTPAPLSSTPVKCDVPPEPEPLSPVKKPAAAPPPPPPPPPPPPPPPGWWHAPIYNSPQARTLTSTNPELLPHPVDPSDMESEDQQLLLQLKRDHTVKDLTQKLSNLPMSPTQDSANRALVGDMSGLISKAKEGLAKSKSKGEISRSSSVDQDVKKSEPKKSENELHWEELVRNMTRPLNLCDLDFTDLRDDDEKDVLAPRSLGAGIPPPPPPLGGAIAPPPMMPPSLAPPPMYGYGGSLTNSVNSLNGSINGELVNGNNTIKKNKKTVKLFWKEVREDMIPQVVGKTIWDELPDANVDTQKLEHLFESRAKDLMTKKQQELNKSKEIIVLDHKRSNAINIAITKLPPPRAIKTAILKMDATVVTREGIDKLLNMLPTDEERGKIQEAQLSNPELPLGSAEQFLLTLASISELEARLKLWAFRLDFDNSEKEIAEPLMDLKQGIEILRQNRTFRSILSTLLSVGIFLNGAPVKGFQIEYLAKVPEVKDTVHKHSLLHHLCHMVMESSSDTSDLYSEIGPITRASKADFTDLAHNLNQLEAECKACWDRLKLIAKHDCPPPLKQKLVDFLADCAERIIILQIVHRRVMNRYRKFLLWLGMPQHSVAESRPNEFCRTLSEFALEYRTTRERVQQQLEKKANHRERNKTRGKLIIDMAKFKTKDDVADDELKKLLDTSSADQADGTLTWRRRRAEQLRSPITRQSEEQFTDGDDEILESLVKTATKAPGTRTTPRERKRTRHADRKSCKLTAQVIRLRSTSKTPIERIRFNEKVLVYIFSNRTQRAMSITSSWILSTRNTHYTIKNSTEKLLYIVIQILISHTKFQIYTSTCSFPYLFTIFSRLRSFCPLSFASLRICCLYWVLIYFHFVLLPLTHQSNKPIKNQIK

Target ORF information:

RefSeq Version	NM_001202143.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster formin homology 2 domain containing, transcript variant D (Fhos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202143.2

ORF Insert Sequence:

ATGATTGTGA AAATGGAGCC GGACGAACTG ATCACATTCC GTGTCCAGTA TCTGGTGGAC 
AGCGATCCGC TCTCGGCGTG CACCGCCTTC CCGATCCCGG TGCGGGCGCC CACCTACGCC 
TTCGCCACCA CCATGCCGCT GGCCAATCAG CTGGGCACAA TCCTGCGGCT GCTGAATGCA 
CCACAAAGCA TCGACGATGC AGCCATTCAG GTGTACAAGG ATGGCGACTA CGGAGCCTAC 
TTGGATCTGG AGTCATCGCT GGCCGAACAG GCCGAGGAAA TCGAGGGAGT CAACGTCAGC 
CGCAAAAACT CTCTGGTGGT GCGAACGCAG TTAAATGTGC GCGTACAAGC CATAATTGAG 
AAACTGCTCT CCGCACAGGG CAGTGACCTG CGGCGTGCTC TCTTCTCGCT GAAGCAGGTG 
TTCCAGGAGG ATAAGGATCT GGTGCACGCC TTCGTGGCCC TCGGCGGCCT CAATTGCCTG 
GTGCGCGTGG GCAATTGTGC GGATCAAAAC TATCAGAATT ACATTCTGCG AGCCCTCGGT 
CAGGTCATGC TCTATGTGGA CGGAATGAAC GGCGTGATGA AGCATGAGCC GACGATGCAG 
TGGCTCTACT CGCTGATTGC CTCCAATTAC CGATCCGTGG TGAAGACCGC CCTCAAGTTG 
TTGCTGGTCT TCGTGGAGTA CGCCGAGTCC AACTGCTATG TGCTGGTCAG TGCCATTCAC 
GCAGTGGATG CTTCCCAGGG CACACTGCCC TGGTCAAATA TAATGAGACT GCTTAAGGAC 
TATGACAATG CTGATGCCGA ACTGGTGATC TATGCCACTT CGCTGATTAA CAAAACTCTG 
GCGGGTCTGA ATGATCAGGA CAGCTTCTAC GACGAAAGCG ATCTTCTAGA GCAGCAGGGC 
ATGGAGACTG TGATCCAGCG TTATATGTCC AAGCCGGGCA CTGATTTGGA TCTGCTCGAT 
CAGTTGCAAC TGTATGAGGC GGTTCTCAAA TTTGAGGATG GTGAGTCGGA TGGACAGCGT 
TTGCCGCAGA ACTCAATGCG AAAAACACAA CGCTATCGTC CGGGAGCAAA TACCACTGAA 
AGACGCAAGT CGCGTCGCCA TAGCACAGAT AATAGTCCCG CATCACTCAC AAAGGTCCTG 
CCCACCACTG TCCTCCGGAT GACACCTACA CAAGCTACGG TGGATGAGGA TAGTTCTGGC 
TCCACCAACT CAACGGAATT TAGTGGCGGT CTCTTTAATG AGAAGAAACC TCGTGATGGT 
GCAGGCGTGA CACCAGGACT TCGAAGAAGA CGTGAGCGGG CGGAGCGGCA TAAGAGCTTT 
CTGAAGGAGC AACAGGAGGC GGCGGCCAAT GGAATCTTTG CTGCTCTCGA GCAGCGGGAG 
GGACTGGGCC AGATTGTAAC CGCCATACCC GCTACCATTC AAACCGGCGA TAGTCCGCCT 
CAGTCCTTGC AACATGAGGT GCCACCGTGC AACAACATCA GCGGAATCAC CAACATTAGT 
AACAACAATG TCAGCAACCT TAGCTGCAAT GACACGCCGA ACAACAGCCT CTTGGTGATG 
AGTCCTAGCA AGCAACTGCT CACTGGAAGC CACAGCATCT CCATTATTAA CAACAGCTTC 
GATAAGGCGA GCAATAACAA TCAGAGCGAG TTCCTGACCA AGAAGAACCT ATTCCGGGAA 
CGCAATTCAA CTGTGATCAA TGGTATTATG AAGAATATCC AGCAAACCGA TAGTGCCTAT 
AAGAAGTACG AGAACGAAGC GAACCGGCTG AACAACTATT ACAGTCAGTC GCCGAAACAT 
CAGATCCATC AGCCCATTCA GCCAACGCCG GAAGTCCTTT TGAGTCCCAA AAAAACGCTA 
AATGCCTCTG GGTTTGATTT CACGCCCGCT CCCCTCAGTT CCACCCCGGT TAAGTGTGAT 
GTCCCGCCGG AACCGGAGCC ACTGAGTCCG GTAAAGAAAC CCGCTGCGGC ACCACCACCG 
CCTCCTCCAC CGCCACCGCC GCCACCACCA CCACCTGGTT GGTGGCACGC CCCCATCTAC 
AACAGCCCGC AAGCTCGAAC CCTAACCAGT ACTAACCCAG AGCTGCTGCC GCATCCTGTG 
GATCCTTCAG ATATGGAAAG CGAGGATCAG CAGCTGCTCC TCCAGCTGAA GCGGGACCAC 
ACGGTCAAGG ATCTCACCCA GAAGTTGAGC AACCTGCCAA TGAGTCCCAC TCAGGATTCG 
GCGAATCGCG CCCTCGTCGG CGATATGTCC GGTCTGATTT CGAAAGCCAA AGAGGGTCTG 
GCCAAGAGCA AGAGCAAGGG AGAGATATCC AGATCTTCTA GTGTCGATCA GGATGTAAAG 
AAGTCTGAGC CCAAGAAGTC GGAAAATGAA CTTCACTGGG AGGAACTGGT TAGGAATATG 
ACAAGGCCCC TAAATCTTTG CGATCTGGAC TTTACGGATC TGAGGGATGA TGACGAGAAG 
GATGTGCTGG CCCCTCGAAG TTTGGGTGCC GGTATACCCC CGCCGCCTCC CCCGCTGGGT 
GGAGCCATTG CCCCACCGCC AATGATGCCT CCAAGCTTGG CACCACCACC CATGTACGGA 
TATGGCGGTA GTCTAACCAA TAGTGTCAAT TCGTTAAATG GTTCTATAAA TGGTGAACTG 
GTCAATGGTA ATAACACCAT CAAAAAGAAC AAGAAAACGG TCAAGCTCTT CTGGAAGGAA 
GTGCGCGAGG ACATGATACC GCAGGTGGTG GGCAAGACCA TATGGGATGA GCTGCCCGAT 
GCCAACGTGG ACACACAGAA GCTCGAACAT CTCTTTGAGT CCCGGGCCAA AGACTTGATG 
ACCAAGAAAC AACAAGAGCT GAACAAGAGC AAAGAGATCA TCGTGCTTGA TCACAAGCGC 
TCCAATGCCA TCAATATTGC GATCACTAAG CTGCCTCCTC CGAGGGCCAT TAAGACGGCC 
ATCCTCAAAA TGGATGCCAC TGTGGTCACA CGGGAGGGAA TCGACAAGCT ACTCAATATG 
CTGCCCACTG ATGAGGAGCG TGGCAAGATC CAGGAGGCCC AGTTATCCAA CCCAGAGTTG 
CCCCTGGGCA GCGCCGAGCA GTTCCTTTTG ACCCTGGCCT CCATTTCGGA GCTGGAAGCT 
CGCCTGAAAT TGTGGGCTTT CCGTCTGGAC TTTGACAACA GTGAGAAAGA GATTGCGGAA 
CCGCTGATGG ACCTCAAGCA AGGCATTGAG ATCCTGCGTC AGAATCGCAC CTTCCGCAGC 
ATCCTCTCTA CTCTGCTGTC TGTGGGCATC TTTTTGAATG GAGCTCCCGT CAAGGGATTC 
CAGATCGAGT ATCTGGCAAA GGTTCCGGAA GTGAAGGACA CGGTGCACAA GCACTCGCTG 
CTGCACCACC TCTGCCACAT GGTCATGGAG TCGAGCAGTG ATACTAGTGA TCTGTACTCC 
GAGATCGGAC CCATTACGCG AGCCTCCAAG GCGGATTTCA CGGACTTGGC ACACAATCTC 
AATCAGTTGG AGGCGGAGTG CAAGGCATGC TGGGATCGTC TGAAACTTAT TGCCAAACAC 
GACTGCCCGC CACCGTTAAA GCAGAAGCTG GTGGATTTCT TGGCCGATTG TGCGGAGCGC 
ATCATCATCC TGCAAATTGT CCATCGACGG GTGATGAATC GCTACCGGAA GTTCCTTTTG 
TGGCTGGGAA TGCCGCAGCA CAGTGTCGCG GAATCGCGTC CCAACGAATT CTGCCGGACA 
CTCTCCGAGT TCGCCCTGGA ATACCGCACC ACTAGAGAGC GGGTTCAGCA GCAGCTGGAG 
AAGAAGGCCA ACCACCGGGA ACGGAACAAG ACCCGCGGCA AACTGATCAT CGATATGGCC 
AAGTTCAAGA CCAAAGATGA CGTGGCCGAT GACGAGTTGA AAAAACTTTT GGACACATCG 
AGTGCCGACC AGGCCGATGG GACACTCACC TGGCGGAGGC GACGAGCGGA ACAGCTCCGC 
TCACCAATTA CCCGCCAAAG CGAGGAGCAG TTCACCGATG GCGACGATGA GATACTGGAA 
TCTCTGGTTA AGACCGCCAC CAAGGCTCCA GGAACACGGA CAACGCCGAG GGAACGGAAA 
CGGACGCGCC ACGCGGATCG CAAATCATGT AAGTTGACCG CACAAGTAAT ACGATTGAGA 
TCCACGTCCA AAACTCCGAT AGAGAGAATA CGTTTTAATG AAAAGGTGTT GGTCTATATC 
TTTTCGAATC GCACACAGCG CGCTATGTCT ATAACCTCTA GCTGGATACT TTCAACCAGA 
AACACACACT ACACCATCAA GAATTCCACA GAGAAACTAC TATATATAGT TATACAAATA 
CTTATTTCCC ACACAAAATT CCAAATTTAC ACAAGTACCT GCAGTTTCCC ATACTTATTT 
ACAATATTTT CCCGATTACG CAGCTTTTGT CCCCTTAGTT TTGCCAGCTT ACGAATCTGT 
TGCCTATATT GGGTTTTAAT TTATTTCCAT TTCGTTCTTT TACCATTAAC GCACCAATCC 
AACAAACCAA TAAAAAATCA AATAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhos ( NM_001202142.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189071.1 Drosophila melanogaster formin homology 2 domain containing, transcript variant E (Fhos), mRNA.

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CloneID	OFa20760
Clone ID Related Accession (Same CDS sequence)	NM_001202142.2
Accession Version	NM_001202142.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	formin homology 2 domain containing, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202142.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGTGA AAATGGAGCC GGACGAACTG ATCACATTCC GTGTCCAGTA TCTGGTGGAC 
AGCGATCCGC TCTCGGCGTG CACCGCCTTC CCGATCCCGG TGCGGGCGCC CACCTACGCC 
TTCGCCACCA CCATGCCGCT GGCCAATCAG CTGGGCACAA TCCTGCGGCT GCTGAATGCA 
CCACAAAGCA TCGACGATGC AGCCATTCAG GTGTACAAGG ATGGCGACTA CGGAGCCTAC 
TTGGATCTGG AGTCATCGCT GGCCGAACAG GCCGAGGAAA TCGAGGGAGT CAACGTCAGC 
CGCAAAAACT CTCTGGTGGT GCGAACGCAG TTAAATGTGC GCGTACAAGC CATAATTGAG 
AAACTGCTCT CCGCACAGGG CAGTGACCTG CGGCGTGCTC TCTTCTCGCT GAAGCAGGTG 
TTCCAGGAGG ATAAGGATCT GGTGCACGCC TTCGTGGCCC TCGGCGGCCT CAATTGCCTG 
GTGCGCGTGG GCAATTGTGC GGATCAAAAC TATCAGAATT ACATTCTGCG AGCCCTCGGT 
CAGGTCATGC TCTATGTGGA CGGAATGAAC GGCGTGATGA AGCATGAGCC GACGATGCAG 
TGGCTCTACT CGCTGATTGC CTCCAATTAC CGATCCGTGG TGAAGACCGC CCTCAAGTTG 
TTGCTGGTCT TCGTGGAGTA CGCCGAGTCC AACTGCTATG TGCTGGTCAG TGCCATTCAC 
GCAGTGGATG CTTCCCAGGG CACACTGCCC TGGTCAAATA TAATGAGACT GCTTAAGGAC 
TATGACAATG CTGATGCCGA ACTGGTGATC TATGCCACTT CGCTGATTAA CAAAACTCTG 
GCGGGTCTGA ATGATCAGGA CAGCTTCTAC GACGAAAGCG ATCTTCTAGA GCAGCAGGGC 
ATGGAGACTG TGATCCAGCG TTATATGTCC AAGCCGGGCA CTGATTTGGA TCTGCTCGAT 
CAGTTGCAAC TGTATGAGGC GGTTCTCAAA TTTGAGGATG GTGAGTCGGA TGGACAGCGT 
TTGCCGCAGA ACTCAATGCG AAAAACACAA CGCTATCGTC CGGGAGCAAA TACCACTGAA 
AGACGCAAGT CGCGTCGCCA TAGCACAGAT AATAGTCCCG CATCACTCAC AAAGGTCCTG 
CCCACCACTG TCCTCCGGAT GACACCTACA CAAGCTACGG TGGATGAGGA TAGTTCTGGC 
TCCACCAACT CAACGGAATT TAGTGGCGGT CTCTTTAATG AGAAGAAACC TCGTGATGGT 
GCAGGCGTGA CACCAGGACT TCGAAGAAGA CGTGAGCGGG CGGAGCGGCA TAAGAGCTTT 
CTGAAGGAGC AACAGGAGGC GGCGGCCAAT GGAATCTTTG CTGCTCTCGA GCAGCGGGAG 
GGACTGGGCC AGATTGTAAC CGCCATACCC GCTACCATTC AAACCGGCGA TAGTCCGCCT 
CAGTCCTTGC AACATGAGGT GCCACCGTGC AACAACATCA GCGGAATCAC CAACATTAGT 
AACAACAATG TCAGCAACCT TAGCTGCAAT GACACGCCGA ACAACAGCCT CTTGGTGATG 
AGTCCTAGCA AGCAACTGCT CACTGGAAGC CACAGCATCT CCATTATTAA CAACAGCTTC 
GATAAGGCGA GCAATAACAA TCAGAGCGAG TTCCTGACCA AGAAGAACCT ATTCCGGGAA 
CGCAATTCAA CTGTGATCAA TGGTATTATG AAGAATATCC AGCAAACCGA TAGTGCCTAT 
AAGAAGTACG AGAACGAAGC GAACCGGCTG AACAACTATT ACAGTCAGTC GCCGAAACAT 
CAGATCCATC AGCCCATTCA GCCAACGCCG GAAGTCCTTT TGAGTCCCAA AAAAACGCTA 
AATGCCTCTG GGTTTGATTT CACGCCCGCT CCCCTCAGTT CCACCCCGGT TAAGTGTGAT 
GTCCCGCCGG AACCGGAGCC ACTGAGTCCG GTAAAGAAAC CCGCTGCGGC ACCACCACCG 
CCTCCTCCAC CGCCACCGCC GCCACCACCA CCACCTGGTT GGTGGCACGC CCCCATCTAC 
AACAGCCCGC AAGCTCGAAC CCTAACCAGT ACTAACCCAG AGCTGCTGCC GCATCCTGTG 
GATCCTTCAG ATATGGAAAG CGAGGATCAG CAGCTGCTCC TCCAGCTGAA GCGGGACCAC 
ACGGTCAAGG ATCTCACCCA GAAGTTGAGC AACCTGCCAA TGAGTCCCAC TCAGGATTCG 
GCGAATCGCG CCCTCGTCGG CGATATGTCC GGTCTGATTT CGAAAGCCAA AGAGGGTCTG 
GCCAAGAGCA AGAGCAAGGG AGAGATATCC AGATCTTCTA GTGTCGATCA GGATGTAAAG 
AAGTCTGAGC CCAAGAAGTC GGAAAATGAA CTTCACTGGG AGGAACTGGT TAGGAATATG 
ACAAGGCCCC TAAATCTTTG CGATCTGGAC TTTACGGATC TGAGGGATGA TGACGAGAAG 
GATGTGCTGG CCCCTCGAAG TTTGGGTGCC GGTATACCCC CGCCGCCTCC CCCGCTGGGT 
GGAGCCATTG CCCCACCGCC AATGATGCCT CCAAGCTTGG CACCACCACC CATGTACGGA 
TATGGCGGTA GTCTAACCAA TAGTGTCAAT TCGTTAAATG GTTCTATAAA TGGTGAACTG 
GTCAATGGTA ATAACACCAT CAAAAAGAAC AAGAAAACGG TCAAGCTCTT CTGGAAGGAA 
GTGCGCGAGG ACATGATACC GCAGGTGGTG GGCAAGACCA TATGGGATGA GCTGCCCGAT 
GCCAACGTGG ACACACAGAA GCTCGAACAT CTCTTTGAGT CCCGGGCCAA AGACTTGATG 
ACCAAGAAAC AACAAGAGCT GAACAAGAGC AAAGAGATCA TCGTGCTTGA TCACAAGCGC 
TCCAATGCCA TCAATATTGC GATCACTAAG CTGCCTCCTC CGAGGGCCAT TAAGACGGCC 
ATCCTCAAAA TGGATGCCAC TGTGGTCACA CGGGAGGGAA TCGACAAGCT ACTCAATATG 
CTGCCCACTG ATGAGGAGCG TGGCAAGATC CAGGAGGCCC AGTTATCCAA CCCAGAGTTG 
CCCCTGGGCA GCGCCGAGCA GTTCCTTTTG ACCCTGGCCT CCATTTCGGA GCTGGAAGCT 
CGCCTGAAAT TGTGGGCTTT CCGTCTGGAC TTTGACAACA GTGAGAAAGA GATTGCGGAA 
CCGCTGATGG ACCTCAAGCA AGGCATTGAG ATCCTGCGTC AGAATCGCAC CTTCCGCAGC 
ATCCTCTCTA CTCTGCTGTC TGTGGGCATC TTTTTGAATG GAGCTCCCGT CAAGGGATTC 
CAGATCGAGT ATCTGGCAAA GGTTCCGGAA GTGAAGGACA CGGTGCACAA GCACTCGCTG 
CTGCACCACC TCTGCCACAT GGTCATGGAG TCGAGCAGTG ATACTAGTGA TCTGTACTCC 
GAGATCGGAC CCATTACGCG AGCCTCCAAG GCGGATTTCA CGGACTTGGC ACACAATCTC 
AATCAGTTGG AGGCGGAGTG CAAGGCATGC TGGGATCGTC TGAAACTTAT TGCCAAACAC 
GACTGCCCGC CACCGTTAAA GCAGAAGCTG GTGGATTTCT TGGCCGATTG TGCGGAGCGC 
ATCATCATCC TGCAAATTGT CCATCGACGG GTGATGAATC GCTACCGGAA GTTCCTTTTG 
TGGCTGGGAA TGCCGCAGCA CAGTGTCGCG GAATCGCGTC CCAACGAATT CTGCCGGACA 
CTCTCCGAGT TCGCCCTGGA ATACCGCACC ACTAGAGAGC GGGTTCAGCA GCAGCTGGAG 
AAGAAGGCCA ACCACCGGGA ACGGAACAAG ACCCGCGGCA AACTGATCAT CGATATGGCC 
AAGTTCAAGA CCAAAGATGA CGTGGCCGAT GACGAGTTGA AAAAACTTTT GGACACATCG 
AGTGCCGACC AGGCCGATGG GACACTCACC TGGCGGAGGC GACGAGCGGA ACAGCTCCGC 
TCACCAATTA CCCGCCAAAG CGAGGAGCAG TTCACCGATG GCGACGATGA GATACTGGAA 
TCTCTGGTTA AGACCGCCAC CAAGGCTCCA GGAACACGGA CAACGCCGAG GGAACGGAAA 
CGGACGCGCC ACGCGGATCG CAAATCATTG AAGCGCAGCC GCACGCGCGA AGGATTCACA 
GTTGAAAGAG CACCATCGCC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189071.1
CDS	321..4484
Translation	MIVKMEPDELITFRVQYLVDSDPLSACTAFPIPVRAPTYAFATTMPLANQLGTILRLLNAPQSIDDAAIQVYKDGDYGAYLDLESSLAEQAEEIEGVNVSRKNSLVVRTQLNVRVQAIIEKLLSAQGSDLRRALFSLKQVFQEDKDLVHAFVALGGLNCLVRVGNCADQNYQNYILRALGQVMLYVDGMNGVMKHEPTMQWLYSLIASNYRSVVKTALKLLLVFVEYAESNCYVLVSAIHAVDASQGTLPWSNIMRLLKDYDNADAELVIYATSLINKTLAGLNDQDSFYDESDLLEQQGMETVIQRYMSKPGTDLDLLDQLQLYEAVLKFEDGESDGQRLPQNSMRKTQRYRPGANTTERRKSRRHSTDNSPASLTKVLPTTVLRMTPTQATVDEDSSGSTNSTEFSGGLFNEKKPRDGAGVTPGLRRRRERAERHKSFLKEQQEAAANGIFAALEQREGLGQIVTAIPATIQTGDSPPQSLQHEVPPCNNISGITNISNNNVSNLSCNDTPNNSLLVMSPSKQLLTGSHSISIINNSFDKASNNNQSEFLTKKNLFRERNSTVINGIMKNIQQTDSAYKKYENEANRLNNYYSQSPKHQIHQPIQPTPEVLLSPKKTLNASGFDFTPAPLSSTPVKCDVPPEPEPLSPVKKPAAAPPPPPPPPPPPPPPPGWWHAPIYNSPQARTLTSTNPELLPHPVDPSDMESEDQQLLLQLKRDHTVKDLTQKLSNLPMSPTQDSANRALVGDMSGLISKAKEGLAKSKSKGEISRSSSVDQDVKKSEPKKSENELHWEELVRNMTRPLNLCDLDFTDLRDDDEKDVLAPRSLGAGIPPPPPPLGGAIAPPPMMPPSLAPPPMYGYGGSLTNSVNSLNGSINGELVNGNNTIKKNKKTVKLFWKEVREDMIPQVVGKTIWDELPDANVDTQKLEHLFESRAKDLMTKKQQELNKSKEIIVLDHKRSNAINIAITKLPPPRAIKTAILKMDATVVTREGIDKLLNMLPTDEERGKIQEAQLSNPELPLGSAEQFLLTLASISELEARLKLWAFRLDFDNSEKEIAEPLMDLKQGIEILRQNRTFRSILSTLLSVGIFLNGAPVKGFQIEYLAKVPEVKDTVHKHSLLHHLCHMVMESSSDTSDLYSEIGPITRASKADFTDLAHNLNQLEAECKACWDRLKLIAKHDCPPPLKQKLVDFLADCAERIIILQIVHRRVMNRYRKFLLWLGMPQHSVAESRPNEFCRTLSEFALEYRTTRERVQQQLEKKANHRERNKTRGKLIIDMAKFKTKDDVADDELKKLLDTSSADQADGTLTWRRRRAEQLRSPITRQSEEQFTDGDDEILESLVKTATKAPGTRTTPRERKRTRHADRKSLKRSRTREGFTVERAPSP

Target ORF information:

RefSeq Version	NM_001202142.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster formin homology 2 domain containing, transcript variant E (Fhos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202142.2

ORF Insert Sequence:

ATGATTGTGA AAATGGAGCC GGACGAACTG ATCACATTCC GTGTCCAGTA TCTGGTGGAC 
AGCGATCCGC TCTCGGCGTG CACCGCCTTC CCGATCCCGG TGCGGGCGCC CACCTACGCC 
TTCGCCACCA CCATGCCGCT GGCCAATCAG CTGGGCACAA TCCTGCGGCT GCTGAATGCA 
CCACAAAGCA TCGACGATGC AGCCATTCAG GTGTACAAGG ATGGCGACTA CGGAGCCTAC 
TTGGATCTGG AGTCATCGCT GGCCGAACAG GCCGAGGAAA TCGAGGGAGT CAACGTCAGC 
CGCAAAAACT CTCTGGTGGT GCGAACGCAG TTAAATGTGC GCGTACAAGC CATAATTGAG 
AAACTGCTCT CCGCACAGGG CAGTGACCTG CGGCGTGCTC TCTTCTCGCT GAAGCAGGTG 
TTCCAGGAGG ATAAGGATCT GGTGCACGCC TTCGTGGCCC TCGGCGGCCT CAATTGCCTG 
GTGCGCGTGG GCAATTGTGC GGATCAAAAC TATCAGAATT ACATTCTGCG AGCCCTCGGT 
CAGGTCATGC TCTATGTGGA CGGAATGAAC GGCGTGATGA AGCATGAGCC GACGATGCAG 
TGGCTCTACT CGCTGATTGC CTCCAATTAC CGATCCGTGG TGAAGACCGC CCTCAAGTTG 
TTGCTGGTCT TCGTGGAGTA CGCCGAGTCC AACTGCTATG TGCTGGTCAG TGCCATTCAC 
GCAGTGGATG CTTCCCAGGG CACACTGCCC TGGTCAAATA TAATGAGACT GCTTAAGGAC 
TATGACAATG CTGATGCCGA ACTGGTGATC TATGCCACTT CGCTGATTAA CAAAACTCTG 
GCGGGTCTGA ATGATCAGGA CAGCTTCTAC GACGAAAGCG ATCTTCTAGA GCAGCAGGGC 
ATGGAGACTG TGATCCAGCG TTATATGTCC AAGCCGGGCA CTGATTTGGA TCTGCTCGAT 
CAGTTGCAAC TGTATGAGGC GGTTCTCAAA TTTGAGGATG GTGAGTCGGA TGGACAGCGT 
TTGCCGCAGA ACTCAATGCG AAAAACACAA CGCTATCGTC CGGGAGCAAA TACCACTGAA 
AGACGCAAGT CGCGTCGCCA TAGCACAGAT AATAGTCCCG CATCACTCAC AAAGGTCCTG 
CCCACCACTG TCCTCCGGAT GACACCTACA CAAGCTACGG TGGATGAGGA TAGTTCTGGC 
TCCACCAACT CAACGGAATT TAGTGGCGGT CTCTTTAATG AGAAGAAACC TCGTGATGGT 
GCAGGCGTGA CACCAGGACT TCGAAGAAGA CGTGAGCGGG CGGAGCGGCA TAAGAGCTTT 
CTGAAGGAGC AACAGGAGGC GGCGGCCAAT GGAATCTTTG CTGCTCTCGA GCAGCGGGAG 
GGACTGGGCC AGATTGTAAC CGCCATACCC GCTACCATTC AAACCGGCGA TAGTCCGCCT 
CAGTCCTTGC AACATGAGGT GCCACCGTGC AACAACATCA GCGGAATCAC CAACATTAGT 
AACAACAATG TCAGCAACCT TAGCTGCAAT GACACGCCGA ACAACAGCCT CTTGGTGATG 
AGTCCTAGCA AGCAACTGCT CACTGGAAGC CACAGCATCT CCATTATTAA CAACAGCTTC 
GATAAGGCGA GCAATAACAA TCAGAGCGAG TTCCTGACCA AGAAGAACCT ATTCCGGGAA 
CGCAATTCAA CTGTGATCAA TGGTATTATG AAGAATATCC AGCAAACCGA TAGTGCCTAT 
AAGAAGTACG AGAACGAAGC GAACCGGCTG AACAACTATT ACAGTCAGTC GCCGAAACAT 
CAGATCCATC AGCCCATTCA GCCAACGCCG GAAGTCCTTT TGAGTCCCAA AAAAACGCTA 
AATGCCTCTG GGTTTGATTT CACGCCCGCT CCCCTCAGTT CCACCCCGGT TAAGTGTGAT 
GTCCCGCCGG AACCGGAGCC ACTGAGTCCG GTAAAGAAAC CCGCTGCGGC ACCACCACCG 
CCTCCTCCAC CGCCACCGCC GCCACCACCA CCACCTGGTT GGTGGCACGC CCCCATCTAC 
AACAGCCCGC AAGCTCGAAC CCTAACCAGT ACTAACCCAG AGCTGCTGCC GCATCCTGTG 
GATCCTTCAG ATATGGAAAG CGAGGATCAG CAGCTGCTCC TCCAGCTGAA GCGGGACCAC 
ACGGTCAAGG ATCTCACCCA GAAGTTGAGC AACCTGCCAA TGAGTCCCAC TCAGGATTCG 
GCGAATCGCG CCCTCGTCGG CGATATGTCC GGTCTGATTT CGAAAGCCAA AGAGGGTCTG 
GCCAAGAGCA AGAGCAAGGG AGAGATATCC AGATCTTCTA GTGTCGATCA GGATGTAAAG 
AAGTCTGAGC CCAAGAAGTC GGAAAATGAA CTTCACTGGG AGGAACTGGT TAGGAATATG 
ACAAGGCCCC TAAATCTTTG CGATCTGGAC TTTACGGATC TGAGGGATGA TGACGAGAAG 
GATGTGCTGG CCCCTCGAAG TTTGGGTGCC GGTATACCCC CGCCGCCTCC CCCGCTGGGT 
GGAGCCATTG CCCCACCGCC AATGATGCCT CCAAGCTTGG CACCACCACC CATGTACGGA 
TATGGCGGTA GTCTAACCAA TAGTGTCAAT TCGTTAAATG GTTCTATAAA TGGTGAACTG 
GTCAATGGTA ATAACACCAT CAAAAAGAAC AAGAAAACGG TCAAGCTCTT CTGGAAGGAA 
GTGCGCGAGG ACATGATACC GCAGGTGGTG GGCAAGACCA TATGGGATGA GCTGCCCGAT 
GCCAACGTGG ACACACAGAA GCTCGAACAT CTCTTTGAGT CCCGGGCCAA AGACTTGATG 
ACCAAGAAAC AACAAGAGCT GAACAAGAGC AAAGAGATCA TCGTGCTTGA TCACAAGCGC 
TCCAATGCCA TCAATATTGC GATCACTAAG CTGCCTCCTC CGAGGGCCAT TAAGACGGCC 
ATCCTCAAAA TGGATGCCAC TGTGGTCACA CGGGAGGGAA TCGACAAGCT ACTCAATATG 
CTGCCCACTG ATGAGGAGCG TGGCAAGATC CAGGAGGCCC AGTTATCCAA CCCAGAGTTG 
CCCCTGGGCA GCGCCGAGCA GTTCCTTTTG ACCCTGGCCT CCATTTCGGA GCTGGAAGCT 
CGCCTGAAAT TGTGGGCTTT CCGTCTGGAC TTTGACAACA GTGAGAAAGA GATTGCGGAA 
CCGCTGATGG ACCTCAAGCA AGGCATTGAG ATCCTGCGTC AGAATCGCAC CTTCCGCAGC 
ATCCTCTCTA CTCTGCTGTC TGTGGGCATC TTTTTGAATG GAGCTCCCGT CAAGGGATTC 
CAGATCGAGT ATCTGGCAAA GGTTCCGGAA GTGAAGGACA CGGTGCACAA GCACTCGCTG 
CTGCACCACC TCTGCCACAT GGTCATGGAG TCGAGCAGTG ATACTAGTGA TCTGTACTCC 
GAGATCGGAC CCATTACGCG AGCCTCCAAG GCGGATTTCA CGGACTTGGC ACACAATCTC 
AATCAGTTGG AGGCGGAGTG CAAGGCATGC TGGGATCGTC TGAAACTTAT TGCCAAACAC 
GACTGCCCGC CACCGTTAAA GCAGAAGCTG GTGGATTTCT TGGCCGATTG TGCGGAGCGC 
ATCATCATCC TGCAAATTGT CCATCGACGG GTGATGAATC GCTACCGGAA GTTCCTTTTG 
TGGCTGGGAA TGCCGCAGCA CAGTGTCGCG GAATCGCGTC CCAACGAATT CTGCCGGACA 
CTCTCCGAGT TCGCCCTGGA ATACCGCACC ACTAGAGAGC GGGTTCAGCA GCAGCTGGAG 
AAGAAGGCCA ACCACCGGGA ACGGAACAAG ACCCGCGGCA AACTGATCAT CGATATGGCC 
AAGTTCAAGA CCAAAGATGA CGTGGCCGAT GACGAGTTGA AAAAACTTTT GGACACATCG 
AGTGCCGACC AGGCCGATGG GACACTCACC TGGCGGAGGC GACGAGCGGA ACAGCTCCGC 
TCACCAATTA CCCGCCAAAG CGAGGAGCAG TTCACCGATG GCGACGATGA GATACTGGAA 
TCTCTGGTTA AGACCGCCAC CAAGGCTCCA GGAACACGGA CAACGCCGAG GGAACGGAAA 
CGGACGCGCC ACGCGGATCG CAAATCATTG AAGCGCAGCC GCACGCGCGA AGGATTCACA 
GTTGAAAGAG CACCATCGCC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhos ( NM_001169916.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163387.2 Drosophila melanogaster formin homology 2 domain containing, transcript variant J (Fhos), mRNA.

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CloneID	OFa20757
Clone ID Related Accession (Same CDS sequence)	NM_001169916.2 , NM_001274670.1
Accession Version	NM_001169916.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	formin homology 2 domain containing, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001169916.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCCT ACCGCAGTTA TACACCCTGG AAAACAAGCT TGGGCAGTTC CTATAAGCCT 
GGCAGCTCAA CGTATGGAAC CTCCGGAGAC TCCACATCGC GCTACACTTC ACTGGCCACT 
CCGAGCTCCT ACAGACCATC GTTCTCTGGA GGAACGTACC GCCTCAAAAG GGATCCACCA 
CCACCAGTCA CCACAACTCC AGTCAGCAGC TATGTTAGTC GCTATGCGAA GACAGAGCCC 
AAAGACGCAG CTCCGAGTGA GCGAAGTAGT CCAGTTAAGA GGTACACGGG TAATACTACC 
AGCTCCCTCG GGAAATATGG ACGCTCTCGA GATCCCAGTC CAGTGGCCTT AGAGCATACT 
AATCGCAATA AGTCTCGGGA TCCGAGTCCA GTTATTGACA GTGCCAGAAG TAAATTGGGA 
AGCACATATC GTGGAGGGAA TACACCCACA ACCTCCAGTT CATCATCCTC GGCCACCAGA 
AACGCTTACA GCAGTCGCTA TGGAGCGGGT TCTCGGATCT CGGGAAATGG AAGTGCCTTA 
TCTTACTTGA CCACCAGTGA TTTTCATGCC AGAACTGCCA GCAGGGCCAA GGCCAGCAGA 
GAAAGGGAGC GGGAAAAGGA GAAGGAGAAG GAAAAGGAGC AGGAGATAGA GGAGAAGCCC 
AGTGAGACGT CTAATGGCAT AGATAAGTCT CCTGCGAAAC AACAGGTAGA AAAACCAGAG 
AACGATGAAG AACCAAATGA AACCTTCATC ACCATATCCG TCGTAACTAG AGCCACTTCA 
CCCACTCTAC CGGGCAGCAC AACGGTACAA CGCACCCGTC GCATTGATCC AGCCAAAACA 
ATAGAGAAGA CCATCCAAAG ATCCTCTAAA AGGCGTGAGA CAACAGATCA GGAAATCCAG 
TCGGACAGAT TGGATGATTC TACCAGATAT CTTCGATATA GTGCCGGCAG CAGTATAAGT 
TCTACTTATA GTCCACACAG AGATAGGATC AGCAGTCTGC GGTACAGTGC CAGCCCTTTG 
AATAGTTCAG GATCGGCGAA ATCCAGTTCG GTGGGTGAGG GAAAACAGAT TCAACCAATC 
CCAGAGACTG TTTCGCCAGC TAGGACCAAT GGCTCTGTGA GCTCAGCCAA GTCCTCAGAA 
GCGCTGTCTC CTCCAAGAAC CAATGGAAAC GTGAGTTCAG GGAAGTCATC TGAAGCCCTA 
TCTCTACCCA GAAGTAATGG GTGCTCATCA AAGTCCACAG AAGCTGTGTC TCCACCCAGG 
ACAAATGGCT CTTTGAGCTC ATCGAAGTCC ACAAAGTCCA AACTTTCACC ACCTAAGGCA 
ATACGCTTAA ACTCCCGGCA ATCTTCAGTG GAAAACCTAG TAAACAAACC ACCTGCCCCT 
CCCAGCAAGG CGGATTCTCC CACCAAGACA TGCAGCTCCA GTTCAGGTAG TTCGGCAGTT 
AAATGGCCCA ACAAGGACTT CCGCAAATCC TCTCTAAATG TGGGACCCAC TGATCGCCCA 
CGGAAGTCTC GAACTCCTAG TAGTGGCGGA GAGTCCGAGG ATCTACCCAA TGGCAGTAGC 
CTTGAAAGGT CACCCAGTGC CGGGTCCGAT GGAAGTCTAG CCTCGGGTAG TTCCTCAAAG 
AAATCGAGCA AACTGAGCAA TGGCAAGGTG CCAGGCAGTC TGAAGCCAAA GAGTCACAAA 
TCCTGCAGCG AAACCACCAC TGGCAACTCA TCTTCGGCAA CCGCCTCCGC ATCGGACAGT 
GAGCAACGGG TCAAGAGCGT GAAAAAGCAG GCCAAAAAGA AGACCACCAC CAAAGAGGGG 
GGTGCTGGCA AGGTCGCCAA CGCCAACAAG ACAAATAGCA CCACTGAATC ACCGCCAGCC 
GTGGTTGATG TTGTGCCAGC GACGCAGACA AGACTGAAGA AGCTATCGGC GGTATCGAAT 
TTTTTCGCTA GCCGTCAGCA AGACGCAAAT AACGAACCGA TCTTCCTCGA AAGCTCTGAG 
AGTTCGGGCG AACCGGAGGC TCATTCGTTG CCAGTGATCT CGGCAATATC GGGAACTTCA 
AAGATCTCGA AAATTTCTGG GATCAGTCTG ACCACAGATT TGCGAACGAA CGAACCGCGA 
ACGACGCCGT CGAGCGAAGC GAAACAATCA CCCCAAACGA ATTCCCCAAG TTCGAGCACA 
CGTTGCCCGC GGGAAACCAC AACGGAAACG GGTACAAGAT TACCCAGCAG CACCACTAGC 
ACCACCCAGG AGTCAAGTCT CACCACCACT GCCAGTTTGA CGACCTCTGA GCAGGACGAT 
CCTAGTTGGT GGCAAGATAC CAGCTTACAG ATTAACACGG CTTCTGCTTT GGATCAAAAT 
AATAGAGGAG AGATGCCCTC TTACAGGCTG AGACACATTG ACTCTGGGGA GGAGCAGGCC 
TGGTGGCTAC GCCAGGATGA GAATGGCGAT GATGATGACA CACTGGCGGA GGAGACCCTC 
AATACTCTAG ATGATGATCT CCAAGAGCCC AGCAAAAGCA CCTGGTGGAG TCAGGACGAT 
CAGGACTCTA GCAGTCAGGT GAAACTAATG CCCAGGAGTA GGATAACGCC AGAAAAGGAT 
GCCTGGTGGC TGCAGGACGA GGATGAGGCT GCTAAATCTC CCATAGAGAA TGGTGATCTG 
AACAACAACA ATAATAGCGA GCCTCATGTG GAGGTGACTC TAAGGTTACC ATCGAATGGC 
AGCCAAAGGA GCTCCAAAAG TAGTGCTTCC AAGAATGGAA GTAATCCGAA GAGTGGTGGC 
GCCAATCCTT GGTGGCTATC CGGACCAGCC AGAAAGATGT TCAATGTCCA ACGCGTTGAG 
TCGGGCGAAA GAGCGTGGTG GCAAGAGGAA GCCGAGGAGC CACCGCAAGA GGAAAAAGAG 
AGCGAACCAC CACCGGTGAA GCCGCCGACG CCGCCACCGC GCATCATCAA GCACTGGGAG 
TCCGGAGAGC GGGCCTGGTG GTTACAGGAT GAGACAGAAA GCAACCAACT CCCTGTTCAG 
GCTAGCAGCG ATGATGAGGA GCAACCAGTT TTGGTGGTGA TTAATCAGCA GGAGTCTAGG 
GATGAAGTGG ACCTGAGGCT GGCACCACCC ACTTTGGAAT CCCAGGAGCT GAGCAACTCC 
TTCAATTTTG ACGCCCATTC CGAGCGACCT CCTCCACTGG GTCAATGTGC CAGTCCCGTT 
CGCTGTCCCT CGCCCTACGA CAACATTCCA AGATCGCAAC TGCAGGAGCC ACCACCACCA 
CCCACTACCA CGCTGACATC CCTGCAGCCG CGCTTTTCAC CCCAAACCCG GCGAGCCGGC 
GAAAAGCTAT TTATTTCGAG GCATCAAAAC ATCGATGAGC TGCTGGGCGG AGCATGCCGT 
CCCCTCAGCC CGCTCTTCTA TGGAAACAGC AATCCTGCTG AGTCCGAGCT GACTCCTCTA 
GCCGGAAAGA ACATGTTCCT GCTTGAGGAG GTAACACCTG ATCAGGTGCG AATTCACGAC 
AGCACTGCCC AGCTGCCGGT TATCCAGAGA ATGCAGCGCG ATGTCGAGTG GTTGGATAAT 
GGCAGCAACT TTTATGAGCC ACCCGAACAA AGACAACACC AGCCGCCGCG CAGGATCGAC 
GATGCAGCCA TTCAGGTGTA CAAGGATGGC GACTACGGAG CCTACTTGGA TCTGGAGTCA 
TCGCTGGCCG AACAGGCCGA GGAAATCGAG GGAGTCAACG TCAGCCGCAA AAACTCTCTG 
GTGGTGCGAA CGCAGTTAAA TGTGCGCGTA CAAGCCATAA TTGAGAAACT GCTCTCCGCA 
CAGGGCAGTG ACCTGCGGCG TGCTCTCTTC TCGCTGAAGC AGGTGTTCCA GGAGGATAAG 
GATCTGGTGC ACGCCTTCGT GGCCCTCGGC GGCCTCAATT GCCTGGTGCG CGTGGGCAAT 
TGTGCGGATC AAAACTATCA GAATTACATT CTGCGAGCCC TCGGTCAGGT CATGCTCTAT 
GTGGACGGAA TGAACGGCGT GATGAAGCAT GAGCCGACGA TGCAGTGGCT CTACTCGCTG 
ATTGCCTCCA ATTACCGATC CGTGGTGAAG ACCGCCCTCA AGTTGTTGCT GGTCTTCGTG 
GAGTACGCCG AGTCCAACTG CTATGTGCTG GTCAGTGCCA TTCACGCAGT GGATGCTTCC 
CAGGGCACAC TGCCCTGGTC AAATATAATG AGACTGCTTA AGGACTATGA CAATGCTGAT 
GCCGAACTGG TGATCTATGC CACTTCGCTG ATTAACAAAA CTCTGGCGGG TCTGAATGAT 
CAGGACAGCT TCTACGACGA AAGCGATCTT CTAGAGCAGC AGGGCATGGA GACTGTGATC 
CAGCGTTATA TGTCCAAGCC GGGCACTGAT TTGGATCTGC TCGATCAGTT GCAACTGTAT 
GAGGCGGTTC TCAAATTTGA GGATGGTGAG TCGGATGGAC AGCGTTTGCC GCAGAACTCA 
ATGCGAAAAA CACAACGCTA TCGTCCGGGA GCAAATACCA CTGAAAGACG CAAGTCGCGT 
CGCCATAGCA CAGATAATAG TCCCGCATCA CTCACAAAGG TCCTGCCCAC CACTGTCCTC 
CGGATGACAC CTACACAAGC TACGGTGGAT GAGGATAGTT CTGGCTCCAC CAACTCAACG 
GAATTTAGTG GCGGTCTCTT TAATGAGAAG AAACCTCGTG ATGGTGCAGG CGTGACACCA 
GGACTTCGAA GAAGACGTGA GCGGGCGGAG CGGCATAAGA GCTTTCTGAA GGAGCAACAG 
GAGGCGGCGG CCAATGGAAT CTTTGCTGCT CTCGAGCAGC GGGAGGGACT GGGCCAGATT 
GTAACCGCCA TACCCGCTAC CATTCAAACC GGCGATAGTC CGCCTCAGTC CTTGCAACAT 
GAGGTGCCAC CGTGCAACAA CATCAGCGGA ATCACCAACA TTAGTAACAA CAATGTCAGC 
AACCTTAGCT GCAATGACAC GCCGAACAAC AGCCTCTTGG TGATGAGTCC TAGCAAGCAA 
CTGCTCACTG GAAGCCACAG CATCTCCATT ATTAACAACA GCTTCGATAA GGCGAGCAAT 
AACAATCAGA GCGAGTTCCT GACCAAGAAG AACCTATTCC GGGAACGCAA TTCAACTGTG 
ATCAATGGTA TTATGAAGAA TATCCAGCAA ACCGATAGTG CCTATAAGAA GTACGAGAAC 
GAAGCGAACC GGCTGAACAA CTATTACAGT CAGTCGCCGA AACATCAGAT CCATCAGCCC 
ATTCAGCCAA CGCCGGAAGT CCTTTTGAGT CCCAAAAAAA CGCTAAATGC CTCTGGGTTT 
GATTTCACGC CCGCTCCCCT CAGTTCCACC CCGGTTAAGT GTGATGTCCC GCCGGAACCG 
GAGCCACTGA GTCCGGTAAA GAAACCCGCT GCGGCACCAC CACCGCCTCC TCCACCGCCA 
CCGCCGCCAC CACCACCACC TGGTTGGTGG CACGCCCCCA TCTACAACAG CCCGCAAGCT 
CGAACCCTAA CCAGTACTAA CCCAGAGCTG CTGCCGCATC CTGTGGATCC TTCAGATATG 
GAAAGCGAGG ATCAGCAGCT GCTCCTCCAG CTGAAGCGGG ACCACACGGT CAAGGATCTC 
ACCCAGAAGT TGAGCAACCT GCCAATGAGT CCCACTCAGG ATTCGGCGAA TCGCGCCCTC 
GTCGGCGATA TGTCCGGTCT GATTTCGAAA GCCAAAGAGG GTCTGGCCAA GAGCAAGAGC 
AAGGGAGAGA TATCCAGATC TTCTAGTGTC GATCAGGATG TAAAGAAGTC TGAGCCCAAG 
AAGTCGGAAA ATGAACTTCA CTGGGAGGAA CTGGTTAGGA ATATGACAAG GCCCCTAAAT 
CTTTGCGATC TGGACTTTAC GGATCTGAGG GATGATGACG AGAAGGATGT GCTGGCCCCT 
CGAAGTTTGG GTGCCGGTAT ACCCCCGCCG CCTCCCCCGC TGGGTGGAGC CATTGCCCCA 
CCGCCAATGA TGCCTCCAAG CTTGGCACCA CCACCCATGT ACGGATATGG CGGTAGTCTA 
ACCAATAGTG TCAATTCGTT AAATGGTTCT ATAAATGGTG AACTGGTCAA TGGTAATAAC 
ACCATCAAAA AGAACAAGAA AACGGTCAAG CTCTTCTGGA AGGAAGTGCG CGAGGACATG 
ATACCGCAGG TGGTGGGCAA GACCATATGG GATGAGCTGC CCGATGCCAA CGTGGACACA 
CAGAAGCTCG AACATCTCTT TGAGTCCCGG GCCAAAGACT TGATGACCAA GAAACAACAA 
GAGCTGAACA AGAGCAAAGA GATCATCGTG CTTGATCACA AGCGCTCCAA TGCCATCAAT 
ATTGCGATCA CTAAGCTGCC TCCTCCGAGG GCCATTAAGA CGGCCATCCT CAAAATGGAT 
GCCACTGTGG TCACACGGGA GGGAATCGAC AAGCTACTCA ATATGCTGCC CACTGATGAG 
GAGCGTGGCA AGATCCAGGA GGCCCAGTTA TCCAACCCAG AGTTGCCCCT GGGCAGCGCC 
GAGCAGTTCC TTTTGACCCT GGCCTCCATT TCGGAGCTGG AAGCTCGCCT GAAATTGTGG 
GCTTTCCGTC TGGACTTTGA CAACAGTGAG AAAGAGATTG CGGAACCGCT GATGGACCTC 
AAGCAAGGCA TTGAGATCCT GCGTCAGAAT CGCACCTTCC GCAGCATCCT CTCTACTCTG 
CTGTCTGTGG GCATCTTTTT GAATGGAGCT CCCGTCAAGG GATTCCAGAT CGAGTATCTG 
GCAAAGGTTC CGGAAGTGAA GGACACGGTG CACAAGCACT CGCTGCTGCA CCACCTCTGC 
CACATGGTCA TGGAGTCGAG CAGTGATACT AGTGATCTGT ACTCCGAGAT CGGACCCATT 
ACGCGAGCCT CCAAGGCGGA TTTCACGGAC TTGGCACACA ATCTCAATCA GTTGGAGGCG 
GAGTGCAAGG CATGCTGGGA TCGTCTGAAA CTTATTGCCA AACACGACTG CCCGCCACCG 
TTAAAGCAGA AGCTGGTGGA TTTCTTGGCC GATTGTGCGG AGCGCATCAT CATCCTGCAA 
ATTGTCCATC GACGGGTGAT GAATCGCTAC CGGAAGTTCC TTTTGTGGCT GGGAATGCCG 
CAGCACAGTG TCGCGGAATC GCGTCCCAAC GAATTCTGCC GGACACTCTC CGAGTTCGCC 
CTGGAATACC GCACCACTAG AGAGCGGGTT CAGCAGCAGC TGGAGAAGAA GGCCAACCAC 
CGGGAACGGA ACAAGACCCG CGGCAAACTG ATCATCGATA TGGCCAAGTT CAAGACCAAA 
GATGACGTGG CCGATGACGA GTTGAAAAAA CTTTTGGACA CATCGAGTGC CGACCAGGCC 
GATGGGACAC TCACCTGGCG GAGGCGACGA GCGGAACAGC TCCGCTCACC AATTACCCGC 
CAAAGCGAGG AGCAGTTCAC CGATGGCGAC GATGAGATAC TGGAATCTCT GGTTAAGACC 
GCCACCAAGG CTCCAGGAAC ACGGACAACG CCGAGGGAAC GGAAACGGAC GCGCCACGCG 
GATCGCAAAT CATTACGTCG CACGCTGAAA AACGGTCTGA CCGAGGAGGA GAAACAGCAC 
GTGGCTGCCT TGATACAAAC CTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163387.2
CDS	333..7919
Translation	MSSYRSYTPWKTSLGSSYKPGSSTYGTSGDSTSRYTSLATPSSYRPSFSGGTYRLKRDPPPPVTTTPVSSYVSRYAKTEPKDAAPSERSSPVKRYTGNTTSSLGKYGRSRDPSPVALEHTNRNKSRDPSPVIDSARSKLGSTYRGGNTPTTSSSSSSATRNAYSSRYGAGSRISGNGSALSYLTTSDFHARTASRAKASREREREKEKEKEKEQEIEEKPSETSNGIDKSPAKQQVEKPENDEEPNETFITISVVTRATSPTLPGSTTVQRTRRIDPAKTIEKTIQRSSKRRETTDQEIQSDRLDDSTRYLRYSAGSSISSTYSPHRDRISSLRYSASPLNSSGSAKSSSVGEGKQIQPIPETVSPARTNGSVSSAKSSEALSPPRTNGNVSSGKSSEALSLPRSNGCSSKSTEAVSPPRTNGSLSSSKSTKSKLSPPKAIRLNSRQSSVENLVNKPPAPPSKADSPTKTCSSSSGSSAVKWPNKDFRKSSLNVGPTDRPRKSRTPSSGGESEDLPNGSSLERSPSAGSDGSLASGSSSKKSSKLSNGKVPGSLKPKSHKSCSETTTGNSSSATASASDSEQRVKSVKKQAKKKTTTKEGGAGKVANANKTNSTTESPPAVVDVVPATQTRLKKLSAVSNFFASRQQDANNEPIFLESSESSGEPEAHSLPVISAISGTSKISKISGISLTTDLRTNEPRTTPSSEAKQSPQTNSPSSSTRCPRETTTETGTRLPSSTTSTTQESSLTTTASLTTSEQDDPSWWQDTSLQINTASALDQNNRGEMPSYRLRHIDSGEEQAWWLRQDENGDDDDTLAEETLNTLDDDLQEPSKSTWWSQDDQDSSSQVKLMPRSRITPEKDAWWLQDEDEAAKSPIENGDLNNNNNSEPHVEVTLRLPSNGSQRSSKSSASKNGSNPKSGGANPWWLSGPARKMFNVQRVESGERAWWQEEAEEPPQEEKESEPPPVKPPTPPPRIIKHWESGERAWWLQDETESNQLPVQASSDDEEQPVLVVINQQESRDEVDLRLAPPTLESQELSNSFNFDAHSERPPPLGQCASPVRCPSPYDNIPRSQLQEPPPPPTTTLTSLQPRFSPQTRRAGEKLFISRHQNIDELLGGACRPLSPLFYGNSNPAESELTPLAGKNMFLLEEVTPDQVRIHDSTAQLPVIQRMQRDVEWLDNGSNFYEPPEQRQHQPPRRIDDAAIQVYKDGDYGAYLDLESSLAEQAEEIEGVNVSRKNSLVVRTQLNVRVQAIIEKLLSAQGSDLRRALFSLKQVFQEDKDLVHAFVALGGLNCLVRVGNCADQNYQNYILRALGQVMLYVDGMNGVMKHEPTMQWLYSLIASNYRSVVKTALKLLLVFVEYAESNCYVLVSAIHAVDASQGTLPWSNIMRLLKDYDNADAELVIYATSLINKTLAGLNDQDSFYDESDLLEQQGMETVIQRYMSKPGTDLDLLDQLQLYEAVLKFEDGESDGQRLPQNSMRKTQRYRPGANTTERRKSRRHSTDNSPASLTKVLPTTVLRMTPTQATVDEDSSGSTNSTEFSGGLFNEKKPRDGAGVTPGLRRRRERAERHKSFLKEQQEAAANGIFAALEQREGLGQIVTAIPATIQTGDSPPQSLQHEVPPCNNISGITNISNNNVSNLSCNDTPNNSLLVMSPSKQLLTGSHSISIINNSFDKASNNNQSEFLTKKNLFRERNSTVINGIMKNIQQTDSAYKKYENEANRLNNYYSQSPKHQIHQPIQPTPEVLLSPKKTLNASGFDFTPAPLSSTPVKCDVPPEPEPLSPVKKPAAAPPPPPPPPPPPPPPPGWWHAPIYNSPQARTLTSTNPELLPHPVDPSDMESEDQQLLLQLKRDHTVKDLTQKLSNLPMSPTQDSANRALVGDMSGLISKAKEGLAKSKSKGEISRSSSVDQDVKKSEPKKSENELHWEELVRNMTRPLNLCDLDFTDLRDDDEKDVLAPRSLGAGIPPPPPPLGGAIAPPPMMPPSLAPPPMYGYGGSLTNSVNSLNGSINGELVNGNNTIKKNKKTVKLFWKEVREDMIPQVVGKTIWDELPDANVDTQKLEHLFESRAKDLMTKKQQELNKSKEIIVLDHKRSNAINIAITKLPPPRAIKTAILKMDATVVTREGIDKLLNMLPTDEERGKIQEAQLSNPELPLGSAEQFLLTLASISELEARLKLWAFRLDFDNSEKEIAEPLMDLKQGIEILRQNRTFRSILSTLLSVGIFLNGAPVKGFQIEYLAKVPEVKDTVHKHSLLHHLCHMVMESSSDTSDLYSEIGPITRASKADFTDLAHNLNQLEAECKACWDRLKLIAKHDCPPPLKQKLVDFLADCAERIIILQIVHRRVMNRYRKFLLWLGMPQHSVAESRPNEFCRTLSEFALEYRTTRERVQQQLEKKANHRERNKTRGKLIIDMAKFKTKDDVADDELKKLLDTSSADQADGTLTWRRRRAEQLRSPITRQSEEQFTDGDDEILESLVKTATKAPGTRTTPRERKRTRHADRKSLRRTLKNGLTEEEKQHVAALIQTY

Target ORF information:

RefSeq Version	NM_001169916.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster formin homology 2 domain containing, transcript variant J (Fhos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169916.2

ORF Insert Sequence:

ATGTCGTCCT ACCGCAGTTA TACACCCTGG AAAACAAGCT TGGGCAGTTC CTATAAGCCT 
GGCAGCTCAA CGTATGGAAC CTCCGGAGAC TCCACATCGC GCTACACTTC ACTGGCCACT 
CCGAGCTCCT ACAGACCATC GTTCTCTGGA GGAACGTACC GCCTCAAAAG GGATCCACCA 
CCACCAGTCA CCACAACTCC AGTCAGCAGC TATGTTAGTC GCTATGCGAA GACAGAGCCC 
AAAGACGCAG CTCCGAGTGA GCGAAGTAGT CCAGTTAAGA GGTACACGGG TAATACTACC 
AGCTCCCTCG GGAAATATGG ACGCTCTCGA GATCCCAGTC CAGTGGCCTT AGAGCATACT 
AATCGCAATA AGTCTCGGGA TCCGAGTCCA GTTATTGACA GTGCCAGAAG TAAATTGGGA 
AGCACATATC GTGGAGGGAA TACACCCACA ACCTCCAGTT CATCATCCTC GGCCACCAGA 
AACGCTTACA GCAGTCGCTA TGGAGCGGGT TCTCGGATCT CGGGAAATGG AAGTGCCTTA 
TCTTACTTGA CCACCAGTGA TTTTCATGCC AGAACTGCCA GCAGGGCCAA GGCCAGCAGA 
GAAAGGGAGC GGGAAAAGGA GAAGGAGAAG GAAAAGGAGC AGGAGATAGA GGAGAAGCCC 
AGTGAGACGT CTAATGGCAT AGATAAGTCT CCTGCGAAAC AACAGGTAGA AAAACCAGAG 
AACGATGAAG AACCAAATGA AACCTTCATC ACCATATCCG TCGTAACTAG AGCCACTTCA 
CCCACTCTAC CGGGCAGCAC AACGGTACAA CGCACCCGTC GCATTGATCC AGCCAAAACA 
ATAGAGAAGA CCATCCAAAG ATCCTCTAAA AGGCGTGAGA CAACAGATCA GGAAATCCAG 
TCGGACAGAT TGGATGATTC TACCAGATAT CTTCGATATA GTGCCGGCAG CAGTATAAGT 
TCTACTTATA GTCCACACAG AGATAGGATC AGCAGTCTGC GGTACAGTGC CAGCCCTTTG 
AATAGTTCAG GATCGGCGAA ATCCAGTTCG GTGGGTGAGG GAAAACAGAT TCAACCAATC 
CCAGAGACTG TTTCGCCAGC TAGGACCAAT GGCTCTGTGA GCTCAGCCAA GTCCTCAGAA 
GCGCTGTCTC CTCCAAGAAC CAATGGAAAC GTGAGTTCAG GGAAGTCATC TGAAGCCCTA 
TCTCTACCCA GAAGTAATGG GTGCTCATCA AAGTCCACAG AAGCTGTGTC TCCACCCAGG 
ACAAATGGCT CTTTGAGCTC ATCGAAGTCC ACAAAGTCCA AACTTTCACC ACCTAAGGCA 
ATACGCTTAA ACTCCCGGCA ATCTTCAGTG GAAAACCTAG TAAACAAACC ACCTGCCCCT 
CCCAGCAAGG CGGATTCTCC CACCAAGACA TGCAGCTCCA GTTCAGGTAG TTCGGCAGTT 
AAATGGCCCA ACAAGGACTT CCGCAAATCC TCTCTAAATG TGGGACCCAC TGATCGCCCA 
CGGAAGTCTC GAACTCCTAG TAGTGGCGGA GAGTCCGAGG ATCTACCCAA TGGCAGTAGC 
CTTGAAAGGT CACCCAGTGC CGGGTCCGAT GGAAGTCTAG CCTCGGGTAG TTCCTCAAAG 
AAATCGAGCA AACTGAGCAA TGGCAAGGTG CCAGGCAGTC TGAAGCCAAA GAGTCACAAA 
TCCTGCAGCG AAACCACCAC TGGCAACTCA TCTTCGGCAA CCGCCTCCGC ATCGGACAGT 
GAGCAACGGG TCAAGAGCGT GAAAAAGCAG GCCAAAAAGA AGACCACCAC CAAAGAGGGG 
GGTGCTGGCA AGGTCGCCAA CGCCAACAAG ACAAATAGCA CCACTGAATC ACCGCCAGCC 
GTGGTTGATG TTGTGCCAGC GACGCAGACA AGACTGAAGA AGCTATCGGC GGTATCGAAT 
TTTTTCGCTA GCCGTCAGCA AGACGCAAAT AACGAACCGA TCTTCCTCGA AAGCTCTGAG 
AGTTCGGGCG AACCGGAGGC TCATTCGTTG CCAGTGATCT CGGCAATATC GGGAACTTCA 
AAGATCTCGA AAATTTCTGG GATCAGTCTG ACCACAGATT TGCGAACGAA CGAACCGCGA 
ACGACGCCGT CGAGCGAAGC GAAACAATCA CCCCAAACGA ATTCCCCAAG TTCGAGCACA 
CGTTGCCCGC GGGAAACCAC AACGGAAACG GGTACAAGAT TACCCAGCAG CACCACTAGC 
ACCACCCAGG AGTCAAGTCT CACCACCACT GCCAGTTTGA CGACCTCTGA GCAGGACGAT 
CCTAGTTGGT GGCAAGATAC CAGCTTACAG ATTAACACGG CTTCTGCTTT GGATCAAAAT 
AATAGAGGAG AGATGCCCTC TTACAGGCTG AGACACATTG ACTCTGGGGA GGAGCAGGCC 
TGGTGGCTAC GCCAGGATGA GAATGGCGAT GATGATGACA CACTGGCGGA GGAGACCCTC 
AATACTCTAG ATGATGATCT CCAAGAGCCC AGCAAAAGCA CCTGGTGGAG TCAGGACGAT 
CAGGACTCTA GCAGTCAGGT GAAACTAATG CCCAGGAGTA GGATAACGCC AGAAAAGGAT 
GCCTGGTGGC TGCAGGACGA GGATGAGGCT GCTAAATCTC CCATAGAGAA TGGTGATCTG 
AACAACAACA ATAATAGCGA GCCTCATGTG GAGGTGACTC TAAGGTTACC ATCGAATGGC 
AGCCAAAGGA GCTCCAAAAG TAGTGCTTCC AAGAATGGAA GTAATCCGAA GAGTGGTGGC 
GCCAATCCTT GGTGGCTATC CGGACCAGCC AGAAAGATGT TCAATGTCCA ACGCGTTGAG 
TCGGGCGAAA GAGCGTGGTG GCAAGAGGAA GCCGAGGAGC CACCGCAAGA GGAAAAAGAG 
AGCGAACCAC CACCGGTGAA GCCGCCGACG CCGCCACCGC GCATCATCAA GCACTGGGAG 
TCCGGAGAGC GGGCCTGGTG GTTACAGGAT GAGACAGAAA GCAACCAACT CCCTGTTCAG 
GCTAGCAGCG ATGATGAGGA GCAACCAGTT TTGGTGGTGA TTAATCAGCA GGAGTCTAGG 
GATGAAGTGG ACCTGAGGCT GGCACCACCC ACTTTGGAAT CCCAGGAGCT GAGCAACTCC 
TTCAATTTTG ACGCCCATTC CGAGCGACCT CCTCCACTGG GTCAATGTGC CAGTCCCGTT 
CGCTGTCCCT CGCCCTACGA CAACATTCCA AGATCGCAAC TGCAGGAGCC ACCACCACCA 
CCCACTACCA CGCTGACATC CCTGCAGCCG CGCTTTTCAC CCCAAACCCG GCGAGCCGGC 
GAAAAGCTAT TTATTTCGAG GCATCAAAAC ATCGATGAGC TGCTGGGCGG AGCATGCCGT 
CCCCTCAGCC CGCTCTTCTA TGGAAACAGC AATCCTGCTG AGTCCGAGCT GACTCCTCTA 
GCCGGAAAGA ACATGTTCCT GCTTGAGGAG GTAACACCTG ATCAGGTGCG AATTCACGAC 
AGCACTGCCC AGCTGCCGGT TATCCAGAGA ATGCAGCGCG ATGTCGAGTG GTTGGATAAT 
GGCAGCAACT TTTATGAGCC ACCCGAACAA AGACAACACC AGCCGCCGCG CAGGATCGAC 
GATGCAGCCA TTCAGGTGTA CAAGGATGGC GACTACGGAG CCTACTTGGA TCTGGAGTCA 
TCGCTGGCCG AACAGGCCGA GGAAATCGAG GGAGTCAACG TCAGCCGCAA AAACTCTCTG 
GTGGTGCGAA CGCAGTTAAA TGTGCGCGTA CAAGCCATAA TTGAGAAACT GCTCTCCGCA 
CAGGGCAGTG ACCTGCGGCG TGCTCTCTTC TCGCTGAAGC AGGTGTTCCA GGAGGATAAG 
GATCTGGTGC ACGCCTTCGT GGCCCTCGGC GGCCTCAATT GCCTGGTGCG CGTGGGCAAT 
TGTGCGGATC AAAACTATCA GAATTACATT CTGCGAGCCC TCGGTCAGGT CATGCTCTAT 
GTGGACGGAA TGAACGGCGT GATGAAGCAT GAGCCGACGA TGCAGTGGCT CTACTCGCTG 
ATTGCCTCCA ATTACCGATC CGTGGTGAAG ACCGCCCTCA AGTTGTTGCT GGTCTTCGTG 
GAGTACGCCG AGTCCAACTG CTATGTGCTG GTCAGTGCCA TTCACGCAGT GGATGCTTCC 
CAGGGCACAC TGCCCTGGTC AAATATAATG AGACTGCTTA AGGACTATGA CAATGCTGAT 
GCCGAACTGG TGATCTATGC CACTTCGCTG ATTAACAAAA CTCTGGCGGG TCTGAATGAT 
CAGGACAGCT TCTACGACGA AAGCGATCTT CTAGAGCAGC AGGGCATGGA GACTGTGATC 
CAGCGTTATA TGTCCAAGCC GGGCACTGAT TTGGATCTGC TCGATCAGTT GCAACTGTAT 
GAGGCGGTTC TCAAATTTGA GGATGGTGAG TCGGATGGAC AGCGTTTGCC GCAGAACTCA 
ATGCGAAAAA CACAACGCTA TCGTCCGGGA GCAAATACCA CTGAAAGACG CAAGTCGCGT 
CGCCATAGCA CAGATAATAG TCCCGCATCA CTCACAAAGG TCCTGCCCAC CACTGTCCTC 
CGGATGACAC CTACACAAGC TACGGTGGAT GAGGATAGTT CTGGCTCCAC CAACTCAACG 
GAATTTAGTG GCGGTCTCTT TAATGAGAAG AAACCTCGTG ATGGTGCAGG CGTGACACCA 
GGACTTCGAA GAAGACGTGA GCGGGCGGAG CGGCATAAGA GCTTTCTGAA GGAGCAACAG 
GAGGCGGCGG CCAATGGAAT CTTTGCTGCT CTCGAGCAGC GGGAGGGACT GGGCCAGATT 
GTAACCGCCA TACCCGCTAC CATTCAAACC GGCGATAGTC CGCCTCAGTC CTTGCAACAT 
GAGGTGCCAC CGTGCAACAA CATCAGCGGA ATCACCAACA TTAGTAACAA CAATGTCAGC 
AACCTTAGCT GCAATGACAC GCCGAACAAC AGCCTCTTGG TGATGAGTCC TAGCAAGCAA 
CTGCTCACTG GAAGCCACAG CATCTCCATT ATTAACAACA GCTTCGATAA GGCGAGCAAT 
AACAATCAGA GCGAGTTCCT GACCAAGAAG AACCTATTCC GGGAACGCAA TTCAACTGTG 
ATCAATGGTA TTATGAAGAA TATCCAGCAA ACCGATAGTG CCTATAAGAA GTACGAGAAC 
GAAGCGAACC GGCTGAACAA CTATTACAGT CAGTCGCCGA AACATCAGAT CCATCAGCCC 
ATTCAGCCAA CGCCGGAAGT CCTTTTGAGT CCCAAAAAAA CGCTAAATGC CTCTGGGTTT 
GATTTCACGC CCGCTCCCCT CAGTTCCACC CCGGTTAAGT GTGATGTCCC GCCGGAACCG 
GAGCCACTGA GTCCGGTAAA GAAACCCGCT GCGGCACCAC CACCGCCTCC TCCACCGCCA 
CCGCCGCCAC CACCACCACC TGGTTGGTGG CACGCCCCCA TCTACAACAG CCCGCAAGCT 
CGAACCCTAA CCAGTACTAA CCCAGAGCTG CTGCCGCATC CTGTGGATCC TTCAGATATG 
GAAAGCGAGG ATCAGCAGCT GCTCCTCCAG CTGAAGCGGG ACCACACGGT CAAGGATCTC 
ACCCAGAAGT TGAGCAACCT GCCAATGAGT CCCACTCAGG ATTCGGCGAA TCGCGCCCTC 
GTCGGCGATA TGTCCGGTCT GATTTCGAAA GCCAAAGAGG GTCTGGCCAA GAGCAAGAGC 
AAGGGAGAGA TATCCAGATC TTCTAGTGTC GATCAGGATG TAAAGAAGTC TGAGCCCAAG 
AAGTCGGAAA ATGAACTTCA CTGGGAGGAA CTGGTTAGGA ATATGACAAG GCCCCTAAAT 
CTTTGCGATC TGGACTTTAC GGATCTGAGG GATGATGACG AGAAGGATGT GCTGGCCCCT 
CGAAGTTTGG GTGCCGGTAT ACCCCCGCCG CCTCCCCCGC TGGGTGGAGC CATTGCCCCA 
CCGCCAATGA TGCCTCCAAG CTTGGCACCA CCACCCATGT ACGGATATGG CGGTAGTCTA 
ACCAATAGTG TCAATTCGTT AAATGGTTCT ATAAATGGTG AACTGGTCAA TGGTAATAAC 
ACCATCAAAA AGAACAAGAA AACGGTCAAG CTCTTCTGGA AGGAAGTGCG CGAGGACATG 
ATACCGCAGG TGGTGGGCAA GACCATATGG GATGAGCTGC CCGATGCCAA CGTGGACACA 
CAGAAGCTCG AACATCTCTT TGAGTCCCGG GCCAAAGACT TGATGACCAA GAAACAACAA 
GAGCTGAACA AGAGCAAAGA GATCATCGTG CTTGATCACA AGCGCTCCAA TGCCATCAAT 
ATTGCGATCA CTAAGCTGCC TCCTCCGAGG GCCATTAAGA CGGCCATCCT CAAAATGGAT 
GCCACTGTGG TCACACGGGA GGGAATCGAC AAGCTACTCA ATATGCTGCC CACTGATGAG 
GAGCGTGGCA AGATCCAGGA GGCCCAGTTA TCCAACCCAG AGTTGCCCCT GGGCAGCGCC 
GAGCAGTTCC TTTTGACCCT GGCCTCCATT TCGGAGCTGG AAGCTCGCCT GAAATTGTGG 
GCTTTCCGTC TGGACTTTGA CAACAGTGAG AAAGAGATTG CGGAACCGCT GATGGACCTC 
AAGCAAGGCA TTGAGATCCT GCGTCAGAAT CGCACCTTCC GCAGCATCCT CTCTACTCTG 
CTGTCTGTGG GCATCTTTTT GAATGGAGCT CCCGTCAAGG GATTCCAGAT CGAGTATCTG 
GCAAAGGTTC CGGAAGTGAA GGACACGGTG CACAAGCACT CGCTGCTGCA CCACCTCTGC 
CACATGGTCA TGGAGTCGAG CAGTGATACT AGTGATCTGT ACTCCGAGAT CGGACCCATT 
ACGCGAGCCT CCAAGGCGGA TTTCACGGAC TTGGCACACA ATCTCAATCA GTTGGAGGCG 
GAGTGCAAGG CATGCTGGGA TCGTCTGAAA CTTATTGCCA AACACGACTG CCCGCCACCG 
TTAAAGCAGA AGCTGGTGGA TTTCTTGGCC GATTGTGCGG AGCGCATCAT CATCCTGCAA 
ATTGTCCATC GACGGGTGAT GAATCGCTAC CGGAAGTTCC TTTTGTGGCT GGGAATGCCG 
CAGCACAGTG TCGCGGAATC GCGTCCCAAC GAATTCTGCC GGACACTCTC CGAGTTCGCC 
CTGGAATACC GCACCACTAG AGAGCGGGTT CAGCAGCAGC TGGAGAAGAA GGCCAACCAC 
CGGGAACGGA ACAAGACCCG CGGCAAACTG ATCATCGATA TGGCCAAGTT CAAGACCAAA 
GATGACGTGG CCGATGACGA GTTGAAAAAA CTTTTGGACA CATCGAGTGC CGACCAGGCC 
GATGGGACAC TCACCTGGCG GAGGCGACGA GCGGAACAGC TCCGCTCACC AATTACCCGC 
CAAAGCGAGG AGCAGTTCAC CGATGGCGAC GATGAGATAC TGGAATCTCT GGTTAAGACC 
GCCACCAAGG CTCCAGGAAC ACGGACAACG CCGAGGGAAC GGAAACGGAC GCGCCACGCG 
GATCGCAAAT CATTACGTCG CACGCTGAAA AACGGTCTGA CCGAGGAGGA GAAACAGCAC 
GTGGCTGCCT TGATACAAAC CTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhos ( NM_001274670.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261599.1 Drosophila melanogaster formin homology 2 domain containing, transcript variant I (Fhos), mRNA.

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CloneID	OFa20757
Clone ID Related Accession (Same CDS sequence)	NM_001169916.2 , NM_001274670.1
Accession Version	NM_001274670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	formin homology 2 domain containing, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCCT ACCGCAGTTA TACACCCTGG AAAACAAGCT TGGGCAGTTC CTATAAGCCT 
GGCAGCTCAA CGTATGGAAC CTCCGGAGAC TCCACATCGC GCTACACTTC ACTGGCCACT 
CCGAGCTCCT ACAGACCATC GTTCTCTGGA GGAACGTACC GCCTCAAAAG GGATCCACCA 
CCACCAGTCA CCACAACTCC AGTCAGCAGC TATGTTAGTC GCTATGCGAA GACAGAGCCC 
AAAGACGCAG CTCCGAGTGA GCGAAGTAGT CCAGTTAAGA GGTACACGGG TAATACTACC 
AGCTCCCTCG GGAAATATGG ACGCTCTCGA GATCCCAGTC CAGTGGCCTT AGAGCATACT 
AATCGCAATA AGTCTCGGGA TCCGAGTCCA GTTATTGACA GTGCCAGAAG TAAATTGGGA 
AGCACATATC GTGGAGGGAA TACACCCACA ACCTCCAGTT CATCATCCTC GGCCACCAGA 
AACGCTTACA GCAGTCGCTA TGGAGCGGGT TCTCGGATCT CGGGAAATGG AAGTGCCTTA 
TCTTACTTGA CCACCAGTGA TTTTCATGCC AGAACTGCCA GCAGGGCCAA GGCCAGCAGA 
GAAAGGGAGC GGGAAAAGGA GAAGGAGAAG GAAAAGGAGC AGGAGATAGA GGAGAAGCCC 
AGTGAGACGT CTAATGGCAT AGATAAGTCT CCTGCGAAAC AACAGGTAGA AAAACCAGAG 
AACGATGAAG AACCAAATGA AACCTTCATC ACCATATCCG TCGTAACTAG AGCCACTTCA 
CCCACTCTAC CGGGCAGCAC AACGGTACAA CGCACCCGTC GCATTGATCC AGCCAAAACA 
ATAGAGAAGA CCATCCAAAG ATCCTCTAAA AGGCGTGAGA CAACAGATCA GGAAATCCAG 
TCGGACAGAT TGGATGATTC TACCAGATAT CTTCGATATA GTGCCGGCAG CAGTATAAGT 
TCTACTTATA GTCCACACAG AGATAGGATC AGCAGTCTGC GGTACAGTGC CAGCCCTTTG 
AATAGTTCAG GATCGGCGAA ATCCAGTTCG GTGGGTGAGG GAAAACAGAT TCAACCAATC 
CCAGAGACTG TTTCGCCAGC TAGGACCAAT GGCTCTGTGA GCTCAGCCAA GTCCTCAGAA 
GCGCTGTCTC CTCCAAGAAC CAATGGAAAC GTGAGTTCAG GGAAGTCATC TGAAGCCCTA 
TCTCTACCCA GAAGTAATGG GTGCTCATCA AAGTCCACAG AAGCTGTGTC TCCACCCAGG 
ACAAATGGCT CTTTGAGCTC ATCGAAGTCC ACAAAGTCCA AACTTTCACC ACCTAAGGCA 
ATACGCTTAA ACTCCCGGCA ATCTTCAGTG GAAAACCTAG TAAACAAACC ACCTGCCCCT 
CCCAGCAAGG CGGATTCTCC CACCAAGACA TGCAGCTCCA GTTCAGGTAG TTCGGCAGTT 
AAATGGCCCA ACAAGGACTT CCGCAAATCC TCTCTAAATG TGGGACCCAC TGATCGCCCA 
CGGAAGTCTC GAACTCCTAG TAGTGGCGGA GAGTCCGAGG ATCTACCCAA TGGCAGTAGC 
CTTGAAAGGT CACCCAGTGC CGGGTCCGAT GGAAGTCTAG CCTCGGGTAG TTCCTCAAAG 
AAATCGAGCA AACTGAGCAA TGGCAAGGTG CCAGGCAGTC TGAAGCCAAA GAGTCACAAA 
TCCTGCAGCG AAACCACCAC TGGCAACTCA TCTTCGGCAA CCGCCTCCGC ATCGGACAGT 
GAGCAACGGG TCAAGAGCGT GAAAAAGCAG GCCAAAAAGA AGACCACCAC CAAAGAGGGG 
GGTGCTGGCA AGGTCGCCAA CGCCAACAAG ACAAATAGCA CCACTGAATC ACCGCCAGCC 
GTGGTTGATG TTGTGCCAGC GACGCAGACA AGACTGAAGA AGCTATCGGC GGTATCGAAT 
TTTTTCGCTA GCCGTCAGCA AGACGCAAAT AACGAACCGA TCTTCCTCGA AAGCTCTGAG 
AGTTCGGGCG AACCGGAGGC TCATTCGTTG CCAGTGATCT CGGCAATATC GGGAACTTCA 
AAGATCTCGA AAATTTCTGG GATCAGTCTG ACCACAGATT TGCGAACGAA CGAACCGCGA 
ACGACGCCGT CGAGCGAAGC GAAACAATCA CCCCAAACGA ATTCCCCAAG TTCGAGCACA 
CGTTGCCCGC GGGAAACCAC AACGGAAACG GGTACAAGAT TACCCAGCAG CACCACTAGC 
ACCACCCAGG AGTCAAGTCT CACCACCACT GCCAGTTTGA CGACCTCTGA GCAGGACGAT 
CCTAGTTGGT GGCAAGATAC CAGCTTACAG ATTAACACGG CTTCTGCTTT GGATCAAAAT 
AATAGAGGAG AGATGCCCTC TTACAGGCTG AGACACATTG ACTCTGGGGA GGAGCAGGCC 
TGGTGGCTAC GCCAGGATGA GAATGGCGAT GATGATGACA CACTGGCGGA GGAGACCCTC 
AATACTCTAG ATGATGATCT CCAAGAGCCC AGCAAAAGCA CCTGGTGGAG TCAGGACGAT 
CAGGACTCTA GCAGTCAGGT GAAACTAATG CCCAGGAGTA GGATAACGCC AGAAAAGGAT 
GCCTGGTGGC TGCAGGACGA GGATGAGGCT GCTAAATCTC CCATAGAGAA TGGTGATCTG 
AACAACAACA ATAATAGCGA GCCTCATGTG GAGGTGACTC TAAGGTTACC ATCGAATGGC 
AGCCAAAGGA GCTCCAAAAG TAGTGCTTCC AAGAATGGAA GTAATCCGAA GAGTGGTGGC 
GCCAATCCTT GGTGGCTATC CGGACCAGCC AGAAAGATGT TCAATGTCCA ACGCGTTGAG 
TCGGGCGAAA GAGCGTGGTG GCAAGAGGAA GCCGAGGAGC CACCGCAAGA GGAAAAAGAG 
AGCGAACCAC CACCGGTGAA GCCGCCGACG CCGCCACCGC GCATCATCAA GCACTGGGAG 
TCCGGAGAGC GGGCCTGGTG GTTACAGGAT GAGACAGAAA GCAACCAACT CCCTGTTCAG 
GCTAGCAGCG ATGATGAGGA GCAACCAGTT TTGGTGGTGA TTAATCAGCA GGAGTCTAGG 
GATGAAGTGG ACCTGAGGCT GGCACCACCC ACTTTGGAAT CCCAGGAGCT GAGCAACTCC 
TTCAATTTTG ACGCCCATTC CGAGCGACCT CCTCCACTGG GTCAATGTGC CAGTCCCGTT 
CGCTGTCCCT CGCCCTACGA CAACATTCCA AGATCGCAAC TGCAGGAGCC ACCACCACCA 
CCCACTACCA CGCTGACATC CCTGCAGCCG CGCTTTTCAC CCCAAACCCG GCGAGCCGGC 
GAAAAGCTAT TTATTTCGAG GCATCAAAAC ATCGATGAGC TGCTGGGCGG AGCATGCCGT 
CCCCTCAGCC CGCTCTTCTA TGGAAACAGC AATCCTGCTG AGTCCGAGCT GACTCCTCTA 
GCCGGAAAGA ACATGTTCCT GCTTGAGGAG GTAACACCTG ATCAGGTGCG AATTCACGAC 
AGCACTGCCC AGCTGCCGGT TATCCAGAGA ATGCAGCGCG ATGTCGAGTG GTTGGATAAT 
GGCAGCAACT TTTATGAGCC ACCCGAACAA AGACAACACC AGCCGCCGCG CAGGATCGAC 
GATGCAGCCA TTCAGGTGTA CAAGGATGGC GACTACGGAG CCTACTTGGA TCTGGAGTCA 
TCGCTGGCCG AACAGGCCGA GGAAATCGAG GGAGTCAACG TCAGCCGCAA AAACTCTCTG 
GTGGTGCGAA CGCAGTTAAA TGTGCGCGTA CAAGCCATAA TTGAGAAACT GCTCTCCGCA 
CAGGGCAGTG ACCTGCGGCG TGCTCTCTTC TCGCTGAAGC AGGTGTTCCA GGAGGATAAG 
GATCTGGTGC ACGCCTTCGT GGCCCTCGGC GGCCTCAATT GCCTGGTGCG CGTGGGCAAT 
TGTGCGGATC AAAACTATCA GAATTACATT CTGCGAGCCC TCGGTCAGGT CATGCTCTAT 
GTGGACGGAA TGAACGGCGT GATGAAGCAT GAGCCGACGA TGCAGTGGCT CTACTCGCTG 
ATTGCCTCCA ATTACCGATC CGTGGTGAAG ACCGCCCTCA AGTTGTTGCT GGTCTTCGTG 
GAGTACGCCG AGTCCAACTG CTATGTGCTG GTCAGTGCCA TTCACGCAGT GGATGCTTCC 
CAGGGCACAC TGCCCTGGTC AAATATAATG AGACTGCTTA AGGACTATGA CAATGCTGAT 
GCCGAACTGG TGATCTATGC CACTTCGCTG ATTAACAAAA CTCTGGCGGG TCTGAATGAT 
CAGGACAGCT TCTACGACGA AAGCGATCTT CTAGAGCAGC AGGGCATGGA GACTGTGATC 
CAGCGTTATA TGTCCAAGCC GGGCACTGAT TTGGATCTGC TCGATCAGTT GCAACTGTAT 
GAGGCGGTTC TCAAATTTGA GGATGGTGAG TCGGATGGAC AGCGTTTGCC GCAGAACTCA 
ATGCGAAAAA CACAACGCTA TCGTCCGGGA GCAAATACCA CTGAAAGACG CAAGTCGCGT 
CGCCATAGCA CAGATAATAG TCCCGCATCA CTCACAAAGG TCCTGCCCAC CACTGTCCTC 
CGGATGACAC CTACACAAGC TACGGTGGAT GAGGATAGTT CTGGCTCCAC CAACTCAACG 
GAATTTAGTG GCGGTCTCTT TAATGAGAAG AAACCTCGTG ATGGTGCAGG CGTGACACCA 
GGACTTCGAA GAAGACGTGA GCGGGCGGAG CGGCATAAGA GCTTTCTGAA GGAGCAACAG 
GAGGCGGCGG CCAATGGAAT CTTTGCTGCT CTCGAGCAGC GGGAGGGACT GGGCCAGATT 
GTAACCGCCA TACCCGCTAC CATTCAAACC GGCGATAGTC CGCCTCAGTC CTTGCAACAT 
GAGGTGCCAC CGTGCAACAA CATCAGCGGA ATCACCAACA TTAGTAACAA CAATGTCAGC 
AACCTTAGCT GCAATGACAC GCCGAACAAC AGCCTCTTGG TGATGAGTCC TAGCAAGCAA 
CTGCTCACTG GAAGCCACAG CATCTCCATT ATTAACAACA GCTTCGATAA GGCGAGCAAT 
AACAATCAGA GCGAGTTCCT GACCAAGAAG AACCTATTCC GGGAACGCAA TTCAACTGTG 
ATCAATGGTA TTATGAAGAA TATCCAGCAA ACCGATAGTG CCTATAAGAA GTACGAGAAC 
GAAGCGAACC GGCTGAACAA CTATTACAGT CAGTCGCCGA AACATCAGAT CCATCAGCCC 
ATTCAGCCAA CGCCGGAAGT CCTTTTGAGT CCCAAAAAAA CGCTAAATGC CTCTGGGTTT 
GATTTCACGC CCGCTCCCCT CAGTTCCACC CCGGTTAAGT GTGATGTCCC GCCGGAACCG 
GAGCCACTGA GTCCGGTAAA GAAACCCGCT GCGGCACCAC CACCGCCTCC TCCACCGCCA 
CCGCCGCCAC CACCACCACC TGGTTGGTGG CACGCCCCCA TCTACAACAG CCCGCAAGCT 
CGAACCCTAA CCAGTACTAA CCCAGAGCTG CTGCCGCATC CTGTGGATCC TTCAGATATG 
GAAAGCGAGG ATCAGCAGCT GCTCCTCCAG CTGAAGCGGG ACCACACGGT CAAGGATCTC 
ACCCAGAAGT TGAGCAACCT GCCAATGAGT CCCACTCAGG ATTCGGCGAA TCGCGCCCTC 
GTCGGCGATA TGTCCGGTCT GATTTCGAAA GCCAAAGAGG GTCTGGCCAA GAGCAAGAGC 
AAGGGAGAGA TATCCAGATC TTCTAGTGTC GATCAGGATG TAAAGAAGTC TGAGCCCAAG 
AAGTCGGAAA ATGAACTTCA CTGGGAGGAA CTGGTTAGGA ATATGACAAG GCCCCTAAAT 
CTTTGCGATC TGGACTTTAC GGATCTGAGG GATGATGACG AGAAGGATGT GCTGGCCCCT 
CGAAGTTTGG GTGCCGGTAT ACCCCCGCCG CCTCCCCCGC TGGGTGGAGC CATTGCCCCA 
CCGCCAATGA TGCCTCCAAG CTTGGCACCA CCACCCATGT ACGGATATGG CGGTAGTCTA 
ACCAATAGTG TCAATTCGTT AAATGGTTCT ATAAATGGTG AACTGGTCAA TGGTAATAAC 
ACCATCAAAA AGAACAAGAA AACGGTCAAG CTCTTCTGGA AGGAAGTGCG CGAGGACATG 
ATACCGCAGG TGGTGGGCAA GACCATATGG GATGAGCTGC CCGATGCCAA CGTGGACACA 
CAGAAGCTCG AACATCTCTT TGAGTCCCGG GCCAAAGACT TGATGACCAA GAAACAACAA 
GAGCTGAACA AGAGCAAAGA GATCATCGTG CTTGATCACA AGCGCTCCAA TGCCATCAAT 
ATTGCGATCA CTAAGCTGCC TCCTCCGAGG GCCATTAAGA CGGCCATCCT CAAAATGGAT 
GCCACTGTGG TCACACGGGA GGGAATCGAC AAGCTACTCA ATATGCTGCC CACTGATGAG 
GAGCGTGGCA AGATCCAGGA GGCCCAGTTA TCCAACCCAG AGTTGCCCCT GGGCAGCGCC 
GAGCAGTTCC TTTTGACCCT GGCCTCCATT TCGGAGCTGG AAGCTCGCCT GAAATTGTGG 
GCTTTCCGTC TGGACTTTGA CAACAGTGAG AAAGAGATTG CGGAACCGCT GATGGACCTC 
AAGCAAGGCA TTGAGATCCT GCGTCAGAAT CGCACCTTCC GCAGCATCCT CTCTACTCTG 
CTGTCTGTGG GCATCTTTTT GAATGGAGCT CCCGTCAAGG GATTCCAGAT CGAGTATCTG 
GCAAAGGTTC CGGAAGTGAA GGACACGGTG CACAAGCACT CGCTGCTGCA CCACCTCTGC 
CACATGGTCA TGGAGTCGAG CAGTGATACT AGTGATCTGT ACTCCGAGAT CGGACCCATT 
ACGCGAGCCT CCAAGGCGGA TTTCACGGAC TTGGCACACA ATCTCAATCA GTTGGAGGCG 
GAGTGCAAGG CATGCTGGGA TCGTCTGAAA CTTATTGCCA AACACGACTG CCCGCCACCG 
TTAAAGCAGA AGCTGGTGGA TTTCTTGGCC GATTGTGCGG AGCGCATCAT CATCCTGCAA 
ATTGTCCATC GACGGGTGAT GAATCGCTAC CGGAAGTTCC TTTTGTGGCT GGGAATGCCG 
CAGCACAGTG TCGCGGAATC GCGTCCCAAC GAATTCTGCC GGACACTCTC CGAGTTCGCC 
CTGGAATACC GCACCACTAG AGAGCGGGTT CAGCAGCAGC TGGAGAAGAA GGCCAACCAC 
CGGGAACGGA ACAAGACCCG CGGCAAACTG ATCATCGATA TGGCCAAGTT CAAGACCAAA 
GATGACGTGG CCGATGACGA GTTGAAAAAA CTTTTGGACA CATCGAGTGC CGACCAGGCC 
GATGGGACAC TCACCTGGCG GAGGCGACGA GCGGAACAGC TCCGCTCACC AATTACCCGC 
CAAAGCGAGG AGCAGTTCAC CGATGGCGAC GATGAGATAC TGGAATCTCT GGTTAAGACC 
GCCACCAAGG CTCCAGGAAC ACGGACAACG CCGAGGGAAC GGAAACGGAC GCGCCACGCG 
GATCGCAAAT CATTACGTCG CACGCTGAAA AACGGTCTGA CCGAGGAGGA GAAACAGCAC 
GTGGCTGCCT TGATACAAAC CTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261599.1
CDS	857..8443
Translation	MSSYRSYTPWKTSLGSSYKPGSSTYGTSGDSTSRYTSLATPSSYRPSFSGGTYRLKRDPPPPVTTTPVSSYVSRYAKTEPKDAAPSERSSPVKRYTGNTTSSLGKYGRSRDPSPVALEHTNRNKSRDPSPVIDSARSKLGSTYRGGNTPTTSSSSSSATRNAYSSRYGAGSRISGNGSALSYLTTSDFHARTASRAKASREREREKEKEKEKEQEIEEKPSETSNGIDKSPAKQQVEKPENDEEPNETFITISVVTRATSPTLPGSTTVQRTRRIDPAKTIEKTIQRSSKRRETTDQEIQSDRLDDSTRYLRYSAGSSISSTYSPHRDRISSLRYSASPLNSSGSAKSSSVGEGKQIQPIPETVSPARTNGSVSSAKSSEALSPPRTNGNVSSGKSSEALSLPRSNGCSSKSTEAVSPPRTNGSLSSSKSTKSKLSPPKAIRLNSRQSSVENLVNKPPAPPSKADSPTKTCSSSSGSSAVKWPNKDFRKSSLNVGPTDRPRKSRTPSSGGESEDLPNGSSLERSPSAGSDGSLASGSSSKKSSKLSNGKVPGSLKPKSHKSCSETTTGNSSSATASASDSEQRVKSVKKQAKKKTTTKEGGAGKVANANKTNSTTESPPAVVDVVPATQTRLKKLSAVSNFFASRQQDANNEPIFLESSESSGEPEAHSLPVISAISGTSKISKISGISLTTDLRTNEPRTTPSSEAKQSPQTNSPSSSTRCPRETTTETGTRLPSSTTSTTQESSLTTTASLTTSEQDDPSWWQDTSLQINTASALDQNNRGEMPSYRLRHIDSGEEQAWWLRQDENGDDDDTLAEETLNTLDDDLQEPSKSTWWSQDDQDSSSQVKLMPRSRITPEKDAWWLQDEDEAAKSPIENGDLNNNNNSEPHVEVTLRLPSNGSQRSSKSSASKNGSNPKSGGANPWWLSGPARKMFNVQRVESGERAWWQEEAEEPPQEEKESEPPPVKPPTPPPRIIKHWESGERAWWLQDETESNQLPVQASSDDEEQPVLVVINQQESRDEVDLRLAPPTLESQELSNSFNFDAHSERPPPLGQCASPVRCPSPYDNIPRSQLQEPPPPPTTTLTSLQPRFSPQTRRAGEKLFISRHQNIDELLGGACRPLSPLFYGNSNPAESELTPLAGKNMFLLEEVTPDQVRIHDSTAQLPVIQRMQRDVEWLDNGSNFYEPPEQRQHQPPRRIDDAAIQVYKDGDYGAYLDLESSLAEQAEEIEGVNVSRKNSLVVRTQLNVRVQAIIEKLLSAQGSDLRRALFSLKQVFQEDKDLVHAFVALGGLNCLVRVGNCADQNYQNYILRALGQVMLYVDGMNGVMKHEPTMQWLYSLIASNYRSVVKTALKLLLVFVEYAESNCYVLVSAIHAVDASQGTLPWSNIMRLLKDYDNADAELVIYATSLINKTLAGLNDQDSFYDESDLLEQQGMETVIQRYMSKPGTDLDLLDQLQLYEAVLKFEDGESDGQRLPQNSMRKTQRYRPGANTTERRKSRRHSTDNSPASLTKVLPTTVLRMTPTQATVDEDSSGSTNSTEFSGGLFNEKKPRDGAGVTPGLRRRRERAERHKSFLKEQQEAAANGIFAALEQREGLGQIVTAIPATIQTGDSPPQSLQHEVPPCNNISGITNISNNNVSNLSCNDTPNNSLLVMSPSKQLLTGSHSISIINNSFDKASNNNQSEFLTKKNLFRERNSTVINGIMKNIQQTDSAYKKYENEANRLNNYYSQSPKHQIHQPIQPTPEVLLSPKKTLNASGFDFTPAPLSSTPVKCDVPPEPEPLSPVKKPAAAPPPPPPPPPPPPPPPGWWHAPIYNSPQARTLTSTNPELLPHPVDPSDMESEDQQLLLQLKRDHTVKDLTQKLSNLPMSPTQDSANRALVGDMSGLISKAKEGLAKSKSKGEISRSSSVDQDVKKSEPKKSENELHWEELVRNMTRPLNLCDLDFTDLRDDDEKDVLAPRSLGAGIPPPPPPLGGAIAPPPMMPPSLAPPPMYGYGGSLTNSVNSLNGSINGELVNGNNTIKKNKKTVKLFWKEVREDMIPQVVGKTIWDELPDANVDTQKLEHLFESRAKDLMTKKQQELNKSKEIIVLDHKRSNAINIAITKLPPPRAIKTAILKMDATVVTREGIDKLLNMLPTDEERGKIQEAQLSNPELPLGSAEQFLLTLASISELEARLKLWAFRLDFDNSEKEIAEPLMDLKQGIEILRQNRTFRSILSTLLSVGIFLNGAPVKGFQIEYLAKVPEVKDTVHKHSLLHHLCHMVMESSSDTSDLYSEIGPITRASKADFTDLAHNLNQLEAECKACWDRLKLIAKHDCPPPLKQKLVDFLADCAERIIILQIVHRRVMNRYRKFLLWLGMPQHSVAESRPNEFCRTLSEFALEYRTTRERVQQQLEKKANHRERNKTRGKLIIDMAKFKTKDDVADDELKKLLDTSSADQADGTLTWRRRRAEQLRSPITRQSEEQFTDGDDEILESLVKTATKAPGTRTTPRERKRTRHADRKSLRRTLKNGLTEEEKQHVAALIQTY

Target ORF information:

RefSeq Version	NM_001274670.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster formin homology 2 domain containing, transcript variant I (Fhos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274670.1

ORF Insert Sequence:

ATGTCGTCCT ACCGCAGTTA TACACCCTGG AAAACAAGCT TGGGCAGTTC CTATAAGCCT 
GGCAGCTCAA CGTATGGAAC CTCCGGAGAC TCCACATCGC GCTACACTTC ACTGGCCACT 
CCGAGCTCCT ACAGACCATC GTTCTCTGGA GGAACGTACC GCCTCAAAAG GGATCCACCA 
CCACCAGTCA CCACAACTCC AGTCAGCAGC TATGTTAGTC GCTATGCGAA GACAGAGCCC 
AAAGACGCAG CTCCGAGTGA GCGAAGTAGT CCAGTTAAGA GGTACACGGG TAATACTACC 
AGCTCCCTCG GGAAATATGG ACGCTCTCGA GATCCCAGTC CAGTGGCCTT AGAGCATACT 
AATCGCAATA AGTCTCGGGA TCCGAGTCCA GTTATTGACA GTGCCAGAAG TAAATTGGGA 
AGCACATATC GTGGAGGGAA TACACCCACA ACCTCCAGTT CATCATCCTC GGCCACCAGA 
AACGCTTACA GCAGTCGCTA TGGAGCGGGT TCTCGGATCT CGGGAAATGG AAGTGCCTTA 
TCTTACTTGA CCACCAGTGA TTTTCATGCC AGAACTGCCA GCAGGGCCAA GGCCAGCAGA 
GAAAGGGAGC GGGAAAAGGA GAAGGAGAAG GAAAAGGAGC AGGAGATAGA GGAGAAGCCC 
AGTGAGACGT CTAATGGCAT AGATAAGTCT CCTGCGAAAC AACAGGTAGA AAAACCAGAG 
AACGATGAAG AACCAAATGA AACCTTCATC ACCATATCCG TCGTAACTAG AGCCACTTCA 
CCCACTCTAC CGGGCAGCAC AACGGTACAA CGCACCCGTC GCATTGATCC AGCCAAAACA 
ATAGAGAAGA CCATCCAAAG ATCCTCTAAA AGGCGTGAGA CAACAGATCA GGAAATCCAG 
TCGGACAGAT TGGATGATTC TACCAGATAT CTTCGATATA GTGCCGGCAG CAGTATAAGT 
TCTACTTATA GTCCACACAG AGATAGGATC AGCAGTCTGC GGTACAGTGC CAGCCCTTTG 
AATAGTTCAG GATCGGCGAA ATCCAGTTCG GTGGGTGAGG GAAAACAGAT TCAACCAATC 
CCAGAGACTG TTTCGCCAGC TAGGACCAAT GGCTCTGTGA GCTCAGCCAA GTCCTCAGAA 
GCGCTGTCTC CTCCAAGAAC CAATGGAAAC GTGAGTTCAG GGAAGTCATC TGAAGCCCTA 
TCTCTACCCA GAAGTAATGG GTGCTCATCA AAGTCCACAG AAGCTGTGTC TCCACCCAGG 
ACAAATGGCT CTTTGAGCTC ATCGAAGTCC ACAAAGTCCA AACTTTCACC ACCTAAGGCA 
ATACGCTTAA ACTCCCGGCA ATCTTCAGTG GAAAACCTAG TAAACAAACC ACCTGCCCCT 
CCCAGCAAGG CGGATTCTCC CACCAAGACA TGCAGCTCCA GTTCAGGTAG TTCGGCAGTT 
AAATGGCCCA ACAAGGACTT CCGCAAATCC TCTCTAAATG TGGGACCCAC TGATCGCCCA 
CGGAAGTCTC GAACTCCTAG TAGTGGCGGA GAGTCCGAGG ATCTACCCAA TGGCAGTAGC 
CTTGAAAGGT CACCCAGTGC CGGGTCCGAT GGAAGTCTAG CCTCGGGTAG TTCCTCAAAG 
AAATCGAGCA AACTGAGCAA TGGCAAGGTG CCAGGCAGTC TGAAGCCAAA GAGTCACAAA 
TCCTGCAGCG AAACCACCAC TGGCAACTCA TCTTCGGCAA CCGCCTCCGC ATCGGACAGT 
GAGCAACGGG TCAAGAGCGT GAAAAAGCAG GCCAAAAAGA AGACCACCAC CAAAGAGGGG 
GGTGCTGGCA AGGTCGCCAA CGCCAACAAG ACAAATAGCA CCACTGAATC ACCGCCAGCC 
GTGGTTGATG TTGTGCCAGC GACGCAGACA AGACTGAAGA AGCTATCGGC GGTATCGAAT 
TTTTTCGCTA GCCGTCAGCA AGACGCAAAT AACGAACCGA TCTTCCTCGA AAGCTCTGAG 
AGTTCGGGCG AACCGGAGGC TCATTCGTTG CCAGTGATCT CGGCAATATC GGGAACTTCA 
AAGATCTCGA AAATTTCTGG GATCAGTCTG ACCACAGATT TGCGAACGAA CGAACCGCGA 
ACGACGCCGT CGAGCGAAGC GAAACAATCA CCCCAAACGA ATTCCCCAAG TTCGAGCACA 
CGTTGCCCGC GGGAAACCAC AACGGAAACG GGTACAAGAT TACCCAGCAG CACCACTAGC 
ACCACCCAGG AGTCAAGTCT CACCACCACT GCCAGTTTGA CGACCTCTGA GCAGGACGAT 
CCTAGTTGGT GGCAAGATAC CAGCTTACAG ATTAACACGG CTTCTGCTTT GGATCAAAAT 
AATAGAGGAG AGATGCCCTC TTACAGGCTG AGACACATTG ACTCTGGGGA GGAGCAGGCC 
TGGTGGCTAC GCCAGGATGA GAATGGCGAT GATGATGACA CACTGGCGGA GGAGACCCTC 
AATACTCTAG ATGATGATCT CCAAGAGCCC AGCAAAAGCA CCTGGTGGAG TCAGGACGAT 
CAGGACTCTA GCAGTCAGGT GAAACTAATG CCCAGGAGTA GGATAACGCC AGAAAAGGAT 
GCCTGGTGGC TGCAGGACGA GGATGAGGCT GCTAAATCTC CCATAGAGAA TGGTGATCTG 
AACAACAACA ATAATAGCGA GCCTCATGTG GAGGTGACTC TAAGGTTACC ATCGAATGGC 
AGCCAAAGGA GCTCCAAAAG TAGTGCTTCC AAGAATGGAA GTAATCCGAA GAGTGGTGGC 
GCCAATCCTT GGTGGCTATC CGGACCAGCC AGAAAGATGT TCAATGTCCA ACGCGTTGAG 
TCGGGCGAAA GAGCGTGGTG GCAAGAGGAA GCCGAGGAGC CACCGCAAGA GGAAAAAGAG 
AGCGAACCAC CACCGGTGAA GCCGCCGACG CCGCCACCGC GCATCATCAA GCACTGGGAG 
TCCGGAGAGC GGGCCTGGTG GTTACAGGAT GAGACAGAAA GCAACCAACT CCCTGTTCAG 
GCTAGCAGCG ATGATGAGGA GCAACCAGTT TTGGTGGTGA TTAATCAGCA GGAGTCTAGG 
GATGAAGTGG ACCTGAGGCT GGCACCACCC ACTTTGGAAT CCCAGGAGCT GAGCAACTCC 
TTCAATTTTG ACGCCCATTC CGAGCGACCT CCTCCACTGG GTCAATGTGC CAGTCCCGTT 
CGCTGTCCCT CGCCCTACGA CAACATTCCA AGATCGCAAC TGCAGGAGCC ACCACCACCA 
CCCACTACCA CGCTGACATC CCTGCAGCCG CGCTTTTCAC CCCAAACCCG GCGAGCCGGC 
GAAAAGCTAT TTATTTCGAG GCATCAAAAC ATCGATGAGC TGCTGGGCGG AGCATGCCGT 
CCCCTCAGCC CGCTCTTCTA TGGAAACAGC AATCCTGCTG AGTCCGAGCT GACTCCTCTA 
GCCGGAAAGA ACATGTTCCT GCTTGAGGAG GTAACACCTG ATCAGGTGCG AATTCACGAC 
AGCACTGCCC AGCTGCCGGT TATCCAGAGA ATGCAGCGCG ATGTCGAGTG GTTGGATAAT 
GGCAGCAACT TTTATGAGCC ACCCGAACAA AGACAACACC AGCCGCCGCG CAGGATCGAC 
GATGCAGCCA TTCAGGTGTA CAAGGATGGC GACTACGGAG CCTACTTGGA TCTGGAGTCA 
TCGCTGGCCG AACAGGCCGA GGAAATCGAG GGAGTCAACG TCAGCCGCAA AAACTCTCTG 
GTGGTGCGAA CGCAGTTAAA TGTGCGCGTA CAAGCCATAA TTGAGAAACT GCTCTCCGCA 
CAGGGCAGTG ACCTGCGGCG TGCTCTCTTC TCGCTGAAGC AGGTGTTCCA GGAGGATAAG 
GATCTGGTGC ACGCCTTCGT GGCCCTCGGC GGCCTCAATT GCCTGGTGCG CGTGGGCAAT 
TGTGCGGATC AAAACTATCA GAATTACATT CTGCGAGCCC TCGGTCAGGT CATGCTCTAT 
GTGGACGGAA TGAACGGCGT GATGAAGCAT GAGCCGACGA TGCAGTGGCT CTACTCGCTG 
ATTGCCTCCA ATTACCGATC CGTGGTGAAG ACCGCCCTCA AGTTGTTGCT GGTCTTCGTG 
GAGTACGCCG AGTCCAACTG CTATGTGCTG GTCAGTGCCA TTCACGCAGT GGATGCTTCC 
CAGGGCACAC TGCCCTGGTC AAATATAATG AGACTGCTTA AGGACTATGA CAATGCTGAT 
GCCGAACTGG TGATCTATGC CACTTCGCTG ATTAACAAAA CTCTGGCGGG TCTGAATGAT 
CAGGACAGCT TCTACGACGA AAGCGATCTT CTAGAGCAGC AGGGCATGGA GACTGTGATC 
CAGCGTTATA TGTCCAAGCC GGGCACTGAT TTGGATCTGC TCGATCAGTT GCAACTGTAT 
GAGGCGGTTC TCAAATTTGA GGATGGTGAG TCGGATGGAC AGCGTTTGCC GCAGAACTCA 
ATGCGAAAAA CACAACGCTA TCGTCCGGGA GCAAATACCA CTGAAAGACG CAAGTCGCGT 
CGCCATAGCA CAGATAATAG TCCCGCATCA CTCACAAAGG TCCTGCCCAC CACTGTCCTC 
CGGATGACAC CTACACAAGC TACGGTGGAT GAGGATAGTT CTGGCTCCAC CAACTCAACG 
GAATTTAGTG GCGGTCTCTT TAATGAGAAG AAACCTCGTG ATGGTGCAGG CGTGACACCA 
GGACTTCGAA GAAGACGTGA GCGGGCGGAG CGGCATAAGA GCTTTCTGAA GGAGCAACAG 
GAGGCGGCGG CCAATGGAAT CTTTGCTGCT CTCGAGCAGC GGGAGGGACT GGGCCAGATT 
GTAACCGCCA TACCCGCTAC CATTCAAACC GGCGATAGTC CGCCTCAGTC CTTGCAACAT 
GAGGTGCCAC CGTGCAACAA CATCAGCGGA ATCACCAACA TTAGTAACAA CAATGTCAGC 
AACCTTAGCT GCAATGACAC GCCGAACAAC AGCCTCTTGG TGATGAGTCC TAGCAAGCAA 
CTGCTCACTG GAAGCCACAG CATCTCCATT ATTAACAACA GCTTCGATAA GGCGAGCAAT 
AACAATCAGA GCGAGTTCCT GACCAAGAAG AACCTATTCC GGGAACGCAA TTCAACTGTG 
ATCAATGGTA TTATGAAGAA TATCCAGCAA ACCGATAGTG CCTATAAGAA GTACGAGAAC 
GAAGCGAACC GGCTGAACAA CTATTACAGT CAGTCGCCGA AACATCAGAT CCATCAGCCC 
ATTCAGCCAA CGCCGGAAGT CCTTTTGAGT CCCAAAAAAA CGCTAAATGC CTCTGGGTTT 
GATTTCACGC CCGCTCCCCT CAGTTCCACC CCGGTTAAGT GTGATGTCCC GCCGGAACCG 
GAGCCACTGA GTCCGGTAAA GAAACCCGCT GCGGCACCAC CACCGCCTCC TCCACCGCCA 
CCGCCGCCAC CACCACCACC TGGTTGGTGG CACGCCCCCA TCTACAACAG CCCGCAAGCT 
CGAACCCTAA CCAGTACTAA CCCAGAGCTG CTGCCGCATC CTGTGGATCC TTCAGATATG 
GAAAGCGAGG ATCAGCAGCT GCTCCTCCAG CTGAAGCGGG ACCACACGGT CAAGGATCTC 
ACCCAGAAGT TGAGCAACCT GCCAATGAGT CCCACTCAGG ATTCGGCGAA TCGCGCCCTC 
GTCGGCGATA TGTCCGGTCT GATTTCGAAA GCCAAAGAGG GTCTGGCCAA GAGCAAGAGC 
AAGGGAGAGA TATCCAGATC TTCTAGTGTC GATCAGGATG TAAAGAAGTC TGAGCCCAAG 
AAGTCGGAAA ATGAACTTCA CTGGGAGGAA CTGGTTAGGA ATATGACAAG GCCCCTAAAT 
CTTTGCGATC TGGACTTTAC GGATCTGAGG GATGATGACG AGAAGGATGT GCTGGCCCCT 
CGAAGTTTGG GTGCCGGTAT ACCCCCGCCG CCTCCCCCGC TGGGTGGAGC CATTGCCCCA 
CCGCCAATGA TGCCTCCAAG CTTGGCACCA CCACCCATGT ACGGATATGG CGGTAGTCTA 
ACCAATAGTG TCAATTCGTT AAATGGTTCT ATAAATGGTG AACTGGTCAA TGGTAATAAC 
ACCATCAAAA AGAACAAGAA AACGGTCAAG CTCTTCTGGA AGGAAGTGCG CGAGGACATG 
ATACCGCAGG TGGTGGGCAA GACCATATGG GATGAGCTGC CCGATGCCAA CGTGGACACA 
CAGAAGCTCG AACATCTCTT TGAGTCCCGG GCCAAAGACT TGATGACCAA GAAACAACAA 
GAGCTGAACA AGAGCAAAGA GATCATCGTG CTTGATCACA AGCGCTCCAA TGCCATCAAT 
ATTGCGATCA CTAAGCTGCC TCCTCCGAGG GCCATTAAGA CGGCCATCCT CAAAATGGAT 
GCCACTGTGG TCACACGGGA GGGAATCGAC AAGCTACTCA ATATGCTGCC CACTGATGAG 
GAGCGTGGCA AGATCCAGGA GGCCCAGTTA TCCAACCCAG AGTTGCCCCT GGGCAGCGCC 
GAGCAGTTCC TTTTGACCCT GGCCTCCATT TCGGAGCTGG AAGCTCGCCT GAAATTGTGG 
GCTTTCCGTC TGGACTTTGA CAACAGTGAG AAAGAGATTG CGGAACCGCT GATGGACCTC 
AAGCAAGGCA TTGAGATCCT GCGTCAGAAT CGCACCTTCC GCAGCATCCT CTCTACTCTG 
CTGTCTGTGG GCATCTTTTT GAATGGAGCT CCCGTCAAGG GATTCCAGAT CGAGTATCTG 
GCAAAGGTTC CGGAAGTGAA GGACACGGTG CACAAGCACT CGCTGCTGCA CCACCTCTGC 
CACATGGTCA TGGAGTCGAG CAGTGATACT AGTGATCTGT ACTCCGAGAT CGGACCCATT 
ACGCGAGCCT CCAAGGCGGA TTTCACGGAC TTGGCACACA ATCTCAATCA GTTGGAGGCG 
GAGTGCAAGG CATGCTGGGA TCGTCTGAAA CTTATTGCCA AACACGACTG CCCGCCACCG 
TTAAAGCAGA AGCTGGTGGA TTTCTTGGCC GATTGTGCGG AGCGCATCAT CATCCTGCAA 
ATTGTCCATC GACGGGTGAT GAATCGCTAC CGGAAGTTCC TTTTGTGGCT GGGAATGCCG 
CAGCACAGTG TCGCGGAATC GCGTCCCAAC GAATTCTGCC GGACACTCTC CGAGTTCGCC 
CTGGAATACC GCACCACTAG AGAGCGGGTT CAGCAGCAGC TGGAGAAGAA GGCCAACCAC 
CGGGAACGGA ACAAGACCCG CGGCAAACTG ATCATCGATA TGGCCAAGTT CAAGACCAAA 
GATGACGTGG CCGATGACGA GTTGAAAAAA CTTTTGGACA CATCGAGTGC CGACCAGGCC 
GATGGGACAC TCACCTGGCG GAGGCGACGA GCGGAACAGC TCCGCTCACC AATTACCCGC 
CAAAGCGAGG AGCAGTTCAC CGATGGCGAC GATGAGATAC TGGAATCTCT GGTTAAGACC 
GCCACCAAGG CTCCAGGAAC ACGGACAACG CCGAGGGAAC GGAAACGGAC GCGCCACGCG 
GATCGCAAAT CATTACGTCG CACGCTGAAA AACGGTCTGA CCGAGGAGGA GAAACAGCAC 
GTGGCTGCCT TGATACAAAC CTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Fhos
Entrez Gene ID	39004
Full Name	Formin homology 2 domain containing
Synonyms	CG32025,CG32030,CG42610,CG5775,CG5797,Dmel\CG42610,Dmel_CG32025,Dmel_CG32030,dFHOS,desert,jaz,knit,l(3)01629
General protein information	Preferred Names Formin homology 2 domain containing Names CG42610 gene product from transcript CG42610-RI CG42610-PA CG42610-PB CG42610-PD CG42610-PE CG42610-PF CG42610-PG CG42610-PH CG42610-PI CG42610-PJ Fhos-PA Fhos-PB Fhos-PD Fhos-PE Fhos-PF Fhos-PG Fhos-PH Fhos-PI Fhos-PJ desert formin homology 2 domain containing formin homology 2 domain containing ortholog jazigo knittrig lethal (3) 01629
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66D12-66D15

Canis lupus familiaris (dog)	FHOD1	XP_005620910.1
Gallus gallus (chicken)	FHOD1	NP_001012792.1
Drosophila melanogaster (fruit fly)	Fhos	NP_729410.1
Caenorhabditis elegans (roundworm)	fhod-1	NP_491513.2
Homo sapiens (human)	FHOD1	NP_037373.2
Pan troglodytes (chimpanzee)	FHOD1	XP_511029.2
Gallus gallus (chicken)	LOC100858420	XP_003643647.2
	FHOD1	XP_001088717.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004873	XP_314313.4
Gallus gallus (chicken)	LOC100857313	XP_004949954.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Fhos cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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