Ncc69 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncc69
Entrez Gene ID	39410
Full Name	sodium chloride cotransporter 69
Synonyms	BEST:CK01027,CG43567,CG4357,CK01027,Dmel\CG4357,NCC69,NKCC,mdcds_25302
General protein information	Preferred Names sodium chloride cotransporter 69 Names CG4357 gene product from transcript CG4357-RA CG4357-PA CG4357-PC CG4357-PD CG4357-PE CG4357-PF CG4357-PG Ncc69-PA Ncc69-PC Ncc69-PD Ncc69-PE Ncc69-PF Ncc69-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 69B3-69B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274830.2	NP_001261759.1	sodium chloride cotransporter 69, isoform F
NM_140315.4	NP_648572.1	sodium chloride cotransporter 69, isoform A
NM_001274827.1	NP_001261756.1	sodium chloride cotransporter 69, isoform C
NM_206344.2	NP_996066.1	sodium chloride cotransporter 69, isoform G
NM_001274828.1	NP_001261757.1	sodium chloride cotransporter 69, isoform D
NM_001274829.1	NP_001261758.1	sodium chloride cotransporter 69, isoform E

Pathways from BioSystems

Homologies

	SLC12A2	XP_001098624.1
Rattus norvegicus (Norway rat)	Slc12a2	NP_113986.1
Danio rerio (zebrafish)	slc12a2	NP_001002080.1
Drosophila melanogaster (fruit fly)	Ncc69	NP_648572.1
Homo sapiens (human)	SLC12A2	NP_001243390.1
Bos taurus (cattle)	SLC12A2	NP_777207.1
Gallus gallus (chicken)	SLC12A2	XP_004949435.1
Pan troglodytes (chimpanzee)	SLC12A2	XP_003310846.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001557	XP_321556.3
Xenopus tropicalis (tropical clawed frog)	slc12a2	XP_004910531.1
Canis lupus familiaris (dog)	SLC12A2	XP_538611.5
Mus musculus (house mouse)	Slc12a2	NP_033220.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Drosophila WNK-SPAK/OSR1-NKCC cascade is an essential molecular pathway for osmoregulation.
Title: Hypotonicity stimulates potassium flux through the WNK-SPAK/OSR1 kinase cascade and the Ncc69 sodium-potassium-2-chloride cotransporter in the Drosophila renal tubule.

Ncc69 plays an important role in transepithelial ion and fluid transport in the fly renal tubule and is a target for regulation in antidiuretic states.
Title: The Drosophila NKCC Ncc69 is required for normal renal tubule function.

This study proposed that normally functioning nerves generate extracellular solutes that are removed by Ncc69 under the control of Fray.
Title: Drosophila glia use a conserved cotransporter mechanism to regulate extracellular volume.

The following Ncc69 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncc69 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18347	NM_140315.4 Latest version!	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa18347	NM_206344.2 Latest version!	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20833	NM_001274828.1 Latest version!	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20833	NM_001274830.2 Latest version!	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20833	NM_001274827.1 Latest version!	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20834	NM_001274829.1 Latest version!	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncc69 ( NM_140315.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648572.1 Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18347
Clone ID Related Accession (Same CDS sequence)	NM_140315.4 , NM_206344.2
Accession Version	NM_140315.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sodium chloride cotransporter 69, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140315.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGCCT CCAGCACGAG TGACCTGTCC TTTATAGCGG GCAATCAATC GAAGGATGTT 
TCCGGCATGC CCGATCCCCT GGATGCCAAA TCGGCCAATC TTGTGAGCAA TTCGTTGCGC 
AAGTCCAAGC TGAAGCACGA TGATCCGGCT TCCCTCTACA AGGGTCCTGG TGGTGCCGAG 
CTGCCCAAGG AGGTACTGGC GGACCTTACC CAATTCACGA GAAAACGCAG CCACGCCGTC 
ATCGATGTCT GGTGGTTGTA CGATGACGGA GGACTCACTC TCCTCTTGCC CTACATCATC 
AGTACGCGGC GCACCTGGCA ATCCTGCAAA TTGAGAGTTT ACGCGCTGGC TAACAAAAAT 
TCGGAATTGG AGTTCGAGCA GCGCTCAATG GCCAGTTTGC TGTCGAAATT CCGGATCGAT 
TACTCGGATT TAACACTGAT TCCGGATATA ACGAAGAAGC CCCAGGAAAC ATCCACGCAG 
TTCTTTAATG AGCTGATTAA GGACTTTGTT GTTACCGAAA AGGATGGCGA GAATGGCACC 
AGCAGCAGGG CAACTCTCAA CGAGGACGAT GCCCTCATAA CCGACGATGA TCTGTTGGCG 
GTGCAGGACA AGACGAATCG GTATTTGCGG CTGCGGGAGT ACCTGCGGGA GCAGTCGACC 
AAGTCGGACC TCGTGGTGAT GACCCTGCCG ATGCCACGGA AGAACATCGT CTCCGCACCC 
CTCTACATGG CCTGGCTGGA GAGCCTGAGT CGGGACATGC CGCCCTTCCT TTTTGTGCGC 
GGCAATCAGA CGAGTGTGCT GACCTTCTAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648572.1
CDS	354..3869
Translation	MSDTISFELGSAADRPPNRFQVNPVNGNSRKSQGADGPGSGSGAGAGAGAGAGEDGPHEVYRRLTNAEGELLEDDTFDATQMLNQRQPRQQRQSIKSSFRDKDKPSRFKDLQTTTRFQVDPQNEESDESNDSQEERELLDNEYDTKYGKSFRHFTREALPRLDNYRNMMSIQAAYRPTLDELHNATLVGKNTHSLTRNQDPESGILNGVLKFGWIKGVLVRCLLNIWGVMLFLRLSWVVGQAGVIEGFVLILTTTAVTTITALSMSAISTNGVIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACAMYVVGFCESMLAMMTTFGWEIIDGGVQDVRIIGCITILLLLIIVVVGMEWEAKAQIGLLIILLVAIGDFVIGSFIGPKSDAEMAKGFLGYNATLFKNNLFADYRPEKGGIQHDFFSVFAIFFPAATGILAGANISGDLKDPQKSIPKGTILAIVITTGTYLIMVLQCGATVARDATGNLSDVVNGTFAFLDCQPGECNYGLQNSFQVIELVSAFGPLIYAGCYAATLSSALASLVSAPKVFQALCKDELYPKIVWFAKGYGKNNEPVRGYVLTFIIACAFILIGELNLIAPLISNFFLAAYMLINFSTFHASLAKPVGWRPTFKYYNMWLSLLGAILCVAVMFLISWATALITFAAVLALYLIVAYRKPDVNWGSTTQAQTYKNALMSVQQLNNVEEHVKNYRPQILVLSGLPNTRPVLVDLAYMLTKNLSLLVCGHVLKGSSSQKYRTYLQERAGNWFRKHRVKGFYALVDGEDFESGTRALMQATGIGKLKPNIILMGYKTDWQTCDHKELDQYFNVMHKALDMYLSVAILRVPQGLDCSQVLGSQDGWKTVSDVPRTLQPNESSGDLQAVDSSVRNGLSGSIDSLSRNVSQASSTSDLSFIAGNQSKDVSGMPDPLDAKSANLVSNSLRKSKLKHDDPASLYKGPGGAELPKEVLADLTQFTRKRSHAVIDVWWLYDDGGLTLLLPYIISTRRTWQSCKLRVYALANKNSELEFEQRSMASLLSKFRIDYSDLTLIPDITKKPQETSTQFFNELIKDFVVTEKDGENGTSSRATLNEDDALITDDDLLAVQDKTNRYLRLREYLREQSTKSDLVVMTLPMPRKNIVSAPLYMAWLESLSRDMPPFLFVRGNQTSVLTFYS

Target ORF information:

RefSeq Version	NM_140315.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140315.4

ORF Insert Sequence:

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGCCT CCAGCACGAG TGACCTGTCC TTTATAGCGG GCAATCAATC GAAGGATGTT 
TCCGGCATGC CCGATCCCCT GGATGCCAAA TCGGCCAATC TTGTGAGCAA TTCGTTGCGC 
AAGTCCAAGC TGAAGCACGA TGATCCGGCT TCCCTCTACA AGGGTCCTGG TGGTGCCGAG 
CTGCCCAAGG AGGTACTGGC GGACCTTACC CAATTCACGA GAAAACGCAG CCACGCCGTC 
ATCGATGTCT GGTGGTTGTA CGATGACGGA GGACTCACTC TCCTCTTGCC CTACATCATC 
AGTACGCGGC GCACCTGGCA ATCCTGCAAA TTGAGAGTTT ACGCGCTGGC TAACAAAAAT 
TCGGAATTGG AGTTCGAGCA GCGCTCAATG GCCAGTTTGC TGTCGAAATT CCGGATCGAT 
TACTCGGATT TAACACTGAT TCCGGATATA ACGAAGAAGC CCCAGGAAAC ATCCACGCAG 
TTCTTTAATG AGCTGATTAA GGACTTTGTT GTTACCGAAA AGGATGGCGA GAATGGCACC 
AGCAGCAGGG CAACTCTCAA CGAGGACGAT GCCCTCATAA CCGACGATGA TCTGTTGGCG 
GTGCAGGACA AGACGAATCG GTATTTGCGG CTGCGGGAGT ACCTGCGGGA GCAGTCGACC 
AAGTCGGACC TCGTGGTGAT GACCCTGCCG ATGCCACGGA AGAACATCGT CTCCGCACCC 
CTCTACATGG CCTGGCTGGA GAGCCTGAGT CGGGACATGC CGCCCTTCCT TTTTGTGCGC 
GGCAATCAGA CGAGTGTGCT GACCTTCTAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncc69 ( NM_206344.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996066.1 Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa18347
Clone ID Related Accession (Same CDS sequence)	NM_140315.4 , NM_206344.2
Accession Version	NM_206344.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sodium chloride cotransporter 69, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206344.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGCCT CCAGCACGAG TGACCTGTCC TTTATAGCGG GCAATCAATC GAAGGATGTT 
TCCGGCATGC CCGATCCCCT GGATGCCAAA TCGGCCAATC TTGTGAGCAA TTCGTTGCGC 
AAGTCCAAGC TGAAGCACGA TGATCCGGCT TCCCTCTACA AGGGTCCTGG TGGTGCCGAG 
CTGCCCAAGG AGGTACTGGC GGACCTTACC CAATTCACGA GAAAACGCAG CCACGCCGTC 
ATCGATGTCT GGTGGTTGTA CGATGACGGA GGACTCACTC TCCTCTTGCC CTACATCATC 
AGTACGCGGC GCACCTGGCA ATCCTGCAAA TTGAGAGTTT ACGCGCTGGC TAACAAAAAT 
TCGGAATTGG AGTTCGAGCA GCGCTCAATG GCCAGTTTGC TGTCGAAATT CCGGATCGAT 
TACTCGGATT TAACACTGAT TCCGGATATA ACGAAGAAGC CCCAGGAAAC ATCCACGCAG 
TTCTTTAATG AGCTGATTAA GGACTTTGTT GTTACCGAAA AGGATGGCGA GAATGGCACC 
AGCAGCAGGG CAACTCTCAA CGAGGACGAT GCCCTCATAA CCGACGATGA TCTGTTGGCG 
GTGCAGGACA AGACGAATCG GTATTTGCGG CTGCGGGAGT ACCTGCGGGA GCAGTCGACC 
AAGTCGGACC TCGTGGTGAT GACCCTGCCG ATGCCACGGA AGAACATCGT CTCCGCACCC 
CTCTACATGG CCTGGCTGGA GAGCCTGAGT CGGGACATGC CGCCCTTCCT TTTTGTGCGC 
GGCAATCAGA CGAGTGTGCT GACCTTCTAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996066.1
CDS	1180..4695
Translation	MSDTISFELGSAADRPPNRFQVNPVNGNSRKSQGADGPGSGSGAGAGAGAGAGEDGPHEVYRRLTNAEGELLEDDTFDATQMLNQRQPRQQRQSIKSSFRDKDKPSRFKDLQTTTRFQVDPQNEESDESNDSQEERELLDNEYDTKYGKSFRHFTREALPRLDNYRNMMSIQAAYRPTLDELHNATLVGKNTHSLTRNQDPESGILNGVLKFGWIKGVLVRCLLNIWGVMLFLRLSWVVGQAGVIEGFVLILTTTAVTTITALSMSAISTNGVIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACAMYVVGFCESMLAMMTTFGWEIIDGGVQDVRIIGCITILLLLIIVVVGMEWEAKAQIGLLIILLVAIGDFVIGSFIGPKSDAEMAKGFLGYNATLFKNNLFADYRPEKGGIQHDFFSVFAIFFPAATGILAGANISGDLKDPQKSIPKGTILAIVITTGTYLIMVLQCGATVARDATGNLSDVVNGTFAFLDCQPGECNYGLQNSFQVIELVSAFGPLIYAGCYAATLSSALASLVSAPKVFQALCKDELYPKIVWFAKGYGKNNEPVRGYVLTFIIACAFILIGELNLIAPLISNFFLAAYMLINFSTFHASLAKPVGWRPTFKYYNMWLSLLGAILCVAVMFLISWATALITFAAVLALYLIVAYRKPDVNWGSTTQAQTYKNALMSVQQLNNVEEHVKNYRPQILVLSGLPNTRPVLVDLAYMLTKNLSLLVCGHVLKGSSSQKYRTYLQERAGNWFRKHRVKGFYALVDGEDFESGTRALMQATGIGKLKPNIILMGYKTDWQTCDHKELDQYFNVMHKALDMYLSVAILRVPQGLDCSQVLGSQDGWKTVSDVPRTLQPNESSGDLQAVDSSVRNGLSGSIDSLSRNVSQASSTSDLSFIAGNQSKDVSGMPDPLDAKSANLVSNSLRKSKLKHDDPASLYKGPGGAELPKEVLADLTQFTRKRSHAVIDVWWLYDDGGLTLLLPYIISTRRTWQSCKLRVYALANKNSELEFEQRSMASLLSKFRIDYSDLTLIPDITKKPQETSTQFFNELIKDFVVTEKDGENGTSSRATLNEDDALITDDDLLAVQDKTNRYLRLREYLREQSTKSDLVVMTLPMPRKNIVSAPLYMAWLESLSRDMPPFLFVRGNQTSVLTFYS

Target ORF information:

RefSeq Version	NM_206344.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206344.2

ORF Insert Sequence:

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGCCT CCAGCACGAG TGACCTGTCC TTTATAGCGG GCAATCAATC GAAGGATGTT 
TCCGGCATGC CCGATCCCCT GGATGCCAAA TCGGCCAATC TTGTGAGCAA TTCGTTGCGC 
AAGTCCAAGC TGAAGCACGA TGATCCGGCT TCCCTCTACA AGGGTCCTGG TGGTGCCGAG 
CTGCCCAAGG AGGTACTGGC GGACCTTACC CAATTCACGA GAAAACGCAG CCACGCCGTC 
ATCGATGTCT GGTGGTTGTA CGATGACGGA GGACTCACTC TCCTCTTGCC CTACATCATC 
AGTACGCGGC GCACCTGGCA ATCCTGCAAA TTGAGAGTTT ACGCGCTGGC TAACAAAAAT 
TCGGAATTGG AGTTCGAGCA GCGCTCAATG GCCAGTTTGC TGTCGAAATT CCGGATCGAT 
TACTCGGATT TAACACTGAT TCCGGATATA ACGAAGAAGC CCCAGGAAAC ATCCACGCAG 
TTCTTTAATG AGCTGATTAA GGACTTTGTT GTTACCGAAA AGGATGGCGA GAATGGCACC 
AGCAGCAGGG CAACTCTCAA CGAGGACGAT GCCCTCATAA CCGACGATGA TCTGTTGGCG 
GTGCAGGACA AGACGAATCG GTATTTGCGG CTGCGGGAGT ACCTGCGGGA GCAGTCGACC 
AAGTCGGACC TCGTGGTGAT GACCCTGCCG ATGCCACGGA AGAACATCGT CTCCGCACCC 
CTCTACATGG CCTGGCTGGA GAGCCTGAGT CGGGACATGC CGCCCTTCCT TTTTGTGCGC 
GGCAATCAGA CGAGTGTGCT GACCTTCTAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncc69 ( NM_001274830.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261759.1 Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant F, mRNA.

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CloneID	OFa20833
Clone ID Related Accession (Same CDS sequence)	NM_001274830.2 , NM_001274827.1 , NM_001274828.1
Accession Version	NM_001274830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sodium chloride cotransporter 69, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_001274830.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGAAG ACCGAAACCG CAACCAGTTG GTCCACAGCG AGCAGAACAG CCTGAAGATA 
GTCAAATCCT CCAGCACGAG TGACCTGTCC TTTATAGCGG GCAATCAATC GAAGGATGTT 
TCCGGCATGC CCGATCCCCT GGATGCCAAA TCGGCCAATC TTGTGAGCAA TTCGTTGCGC 
AAGTCCAAGC TGAAGCACGA TGATCCGGCT TCCCTCTACA AGGGTCCTGG TGGTGCCGAG 
CTGCCCAAGG AGGTACTGGC GGACCTTACC CAATTCACGA GAAAACGCAG CCACGCCGTC 
ATCGATGTCT GGTGGTTGTA CGATGACGGA GGACTCACTC TCCTCTTGCC CTACATCATC 
AGTACGCGGC GCACCTGGCA ATCCTGCAAA TTGAGAGTTT ACGCGCTGGC TAACAAAAAT 
TCGGAATTGG AGTTCGAGCA GCGCTCAATG GCCAGTTTGC TGTCGAAATT CCGGATCGAT 
TACTCGGATT TAACACTGAT TCCGGATATA ACGAAGAAGC CCCAGGAAAC ATCCACGCAG 
TTCTTTAATG AGCTGATTAA GGACTTTGTT GTTACCGAAA AGGATGGCGA GAATGGCACC 
AGCAGCAGGG CAACTCTCAA CGAGGACGAT GCCCTCATAA CCGACGATGA TCTGTTGGCG 
GTGCAGGACA AGACGAATCG GTATTTGCGG CTGCGGGAGT ACCTGCGGGA GCAGTCGACC 
AAGTCGGACC TCGTGGTGAT GACCCTGCCG ATGCCACGGA AGAACATCGT CTCCGCACCC 
CTCTACATGG CCTGGCTGGA GAGCCTGAGT CGGGACATGC CGCCCTTCCT TTTTGTGCGC 
GGCAATCAGA CGAGTGTGCT GACCTTCTAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261759.1
CDS	409..3984
Translation	MSDTISFELGSAADRPPNRFQVNPVNGNSRKSQGADGPGSGSGAGAGAGAGAGEDGPHEVYRRLTNAEGELLEDDTFDATQMLNQRQPRQQRQSIKSSFRDKDKPSRFKDLQTTTRFQVDPQNEESDESNDSQEERELLDNEYDTKYGKSFRHFTREALPRLDNYRNMMSIQAAYRPTLDELHNATLVGKNTHSLTRNQDPESGILNGVLKFGWIKGVLVRCLLNIWGVMLFLRLSWVVGQAGVIEGFVLILTTTAVTTITALSMSAISTNGVIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACAMYVVGFCESMLAMMTTFGWEIIDGGVQDVRIIGCITILLLLIIVVVGMEWEAKAQIGLLIILLVAIGDFVIGSFIGPKSDAEMAKGFLGYNATLFKNNLFADYRPEKGGIQHDFFSVFAIFFPAATGILAGANISGDLKDPQKSIPKGTILAIVITTGTYLIMVLQCGATVARDATGNLSDVVNGTFAFLDCQPGECNYGLQNSFQVIELVSAFGPLIYAGCYAATLSSALASLVSAPKVFQALCKDELYPKIVWFAKGYGKNNEPVRGYVLTFIIACAFILIGELNLIAPLISNFFLAAYMLINFSTFHASLAKPVGWRPTFKYYNMWLSLLGAILCVAVMFLISWATALITFAAVLALYLIVAYRKPDVNWGSTTQAQTYKNALMSVQQLNNVEEHVKNYRPQILVLSGLPNTRPVLVDLAYMLTKNLSLLVCGHVLKGSSSQKYRTYLQERAGNWFRKHRVKGFYALVDGEDFESGTRALMQATGIGKLKPNIILMGYKTDWQTCDHKELDQYFNVMHKALDMYLSVAILRVPQGLDCSQVLGSQDGWKTVSDVPRTLQPNESSGDLQAVDSSVRNGLSGSIDSLSRNVSQEDRNRNQLVHSEQNSLKIVKSSSTSDLSFIAGNQSKDVSGMPDPLDAKSANLVSNSLRKSKLKHDDPASLYKGPGGAELPKEVLADLTQFTRKRSHAVIDVWWLYDDGGLTLLLPYIISTRRTWQSCKLRVYALANKNSELEFEQRSMASLLSKFRIDYSDLTLIPDITKKPQETSTQFFNELIKDFVVTEKDGENGTSSRATLNEDDALITDDDLLAVQDKTNRYLRLREYLREQSTKSDLVVMTLPMPRKNIVSAPLYMAWLESLSRDMPPFLFVRGNQTSVLTFYS

Target ORF information:

RefSeq Version	NM_001274830.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274830.2

ORF Insert Sequence:

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGAAG ACCGAAACCG CAACCAGTTG GTCCACAGCG AGCAGAACAG CCTGAAGATA 
GTCAAATCCT CCAGCACGAG TGACCTGTCC TTTATAGCGG GCAATCAATC GAAGGATGTT 
TCCGGCATGC CCGATCCCCT GGATGCCAAA TCGGCCAATC TTGTGAGCAA TTCGTTGCGC 
AAGTCCAAGC TGAAGCACGA TGATCCGGCT TCCCTCTACA AGGGTCCTGG TGGTGCCGAG 
CTGCCCAAGG AGGTACTGGC GGACCTTACC CAATTCACGA GAAAACGCAG CCACGCCGTC 
ATCGATGTCT GGTGGTTGTA CGATGACGGA GGACTCACTC TCCTCTTGCC CTACATCATC 
AGTACGCGGC GCACCTGGCA ATCCTGCAAA TTGAGAGTTT ACGCGCTGGC TAACAAAAAT 
TCGGAATTGG AGTTCGAGCA GCGCTCAATG GCCAGTTTGC TGTCGAAATT CCGGATCGAT 
TACTCGGATT TAACACTGAT TCCGGATATA ACGAAGAAGC CCCAGGAAAC ATCCACGCAG 
TTCTTTAATG AGCTGATTAA GGACTTTGTT GTTACCGAAA AGGATGGCGA GAATGGCACC 
AGCAGCAGGG CAACTCTCAA CGAGGACGAT GCCCTCATAA CCGACGATGA TCTGTTGGCG 
GTGCAGGACA AGACGAATCG GTATTTGCGG CTGCGGGAGT ACCTGCGGGA GCAGTCGACC 
AAGTCGGACC TCGTGGTGAT GACCCTGCCG ATGCCACGGA AGAACATCGT CTCCGCACCC 
CTCTACATGG CCTGGCTGGA GAGCCTGAGT CGGGACATGC CGCCCTTCCT TTTTGTGCGC 
GGCAATCAGA CGAGTGTGCT GACCTTCTAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncc69 ( NM_001274827.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261756.1 Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant C, mRNA.

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CloneID	OFa20833
Clone ID Related Accession (Same CDS sequence)	NM_001274830.2 , NM_001274827.1 , NM_001274828.1
Accession Version	NM_001274827.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sodium chloride cotransporter 69, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGAAG ACCGAAACCG CAACCAGTTG GTCCACAGCG AGCAGAACAG CCTGAAGATA 
GTCAAATCCT CCAGCACGAG TGACCTGTCC TTTATAGCGG GCAATCAATC GAAGGATGTT 
TCCGGCATGC CCGATCCCCT GGATGCCAAA TCGGCCAATC TTGTGAGCAA TTCGTTGCGC 
AAGTCCAAGC TGAAGCACGA TGATCCGGCT TCCCTCTACA AGGGTCCTGG TGGTGCCGAG 
CTGCCCAAGG AGGTACTGGC GGACCTTACC CAATTCACGA GAAAACGCAG CCACGCCGTC 
ATCGATGTCT GGTGGTTGTA CGATGACGGA GGACTCACTC TCCTCTTGCC CTACATCATC 
AGTACGCGGC GCACCTGGCA ATCCTGCAAA TTGAGAGTTT ACGCGCTGGC TAACAAAAAT 
TCGGAATTGG AGTTCGAGCA GCGCTCAATG GCCAGTTTGC TGTCGAAATT CCGGATCGAT 
TACTCGGATT TAACACTGAT TCCGGATATA ACGAAGAAGC CCCAGGAAAC ATCCACGCAG 
TTCTTTAATG AGCTGATTAA GGACTTTGTT GTTACCGAAA AGGATGGCGA GAATGGCACC 
AGCAGCAGGG CAACTCTCAA CGAGGACGAT GCCCTCATAA CCGACGATGA TCTGTTGGCG 
GTGCAGGACA AGACGAATCG GTATTTGCGG CTGCGGGAGT ACCTGCGGGA GCAGTCGACC 
AAGTCGGACC TCGTGGTGAT GACCCTGCCG ATGCCACGGA AGAACATCGT CTCCGCACCC 
CTCTACATGG CCTGGCTGGA GAGCCTGAGT CGGGACATGC CGCCCTTCCT TTTTGTGCGC 
GGCAATCAGA CGAGTGTGCT GACCTTCTAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261756.1
CDS	1180..4755
Translation	MSDTISFELGSAADRPPNRFQVNPVNGNSRKSQGADGPGSGSGAGAGAGAGAGEDGPHEVYRRLTNAEGELLEDDTFDATQMLNQRQPRQQRQSIKSSFRDKDKPSRFKDLQTTTRFQVDPQNEESDESNDSQEERELLDNEYDTKYGKSFRHFTREALPRLDNYRNMMSIQAAYRPTLDELHNATLVGKNTHSLTRNQDPESGILNGVLKFGWIKGVLVRCLLNIWGVMLFLRLSWVVGQAGVIEGFVLILTTTAVTTITALSMSAISTNGVIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACAMYVVGFCESMLAMMTTFGWEIIDGGVQDVRIIGCITILLLLIIVVVGMEWEAKAQIGLLIILLVAIGDFVIGSFIGPKSDAEMAKGFLGYNATLFKNNLFADYRPEKGGIQHDFFSVFAIFFPAATGILAGANISGDLKDPQKSIPKGTILAIVITTGTYLIMVLQCGATVARDATGNLSDVVNGTFAFLDCQPGECNYGLQNSFQVIELVSAFGPLIYAGCYAATLSSALASLVSAPKVFQALCKDELYPKIVWFAKGYGKNNEPVRGYVLTFIIACAFILIGELNLIAPLISNFFLAAYMLINFSTFHASLAKPVGWRPTFKYYNMWLSLLGAILCVAVMFLISWATALITFAAVLALYLIVAYRKPDVNWGSTTQAQTYKNALMSVQQLNNVEEHVKNYRPQILVLSGLPNTRPVLVDLAYMLTKNLSLLVCGHVLKGSSSQKYRTYLQERAGNWFRKHRVKGFYALVDGEDFESGTRALMQATGIGKLKPNIILMGYKTDWQTCDHKELDQYFNVMHKALDMYLSVAILRVPQGLDCSQVLGSQDGWKTVSDVPRTLQPNESSGDLQAVDSSVRNGLSGSIDSLSRNVSQEDRNRNQLVHSEQNSLKIVKSSSTSDLSFIAGNQSKDVSGMPDPLDAKSANLVSNSLRKSKLKHDDPASLYKGPGGAELPKEVLADLTQFTRKRSHAVIDVWWLYDDGGLTLLLPYIISTRRTWQSCKLRVYALANKNSELEFEQRSMASLLSKFRIDYSDLTLIPDITKKPQETSTQFFNELIKDFVVTEKDGENGTSSRATLNEDDALITDDDLLAVQDKTNRYLRLREYLREQSTKSDLVVMTLPMPRKNIVSAPLYMAWLESLSRDMPPFLFVRGNQTSVLTFYS

Target ORF information:

RefSeq Version	NM_001274827.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274827.1

ORF Insert Sequence:

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGAAG ACCGAAACCG CAACCAGTTG GTCCACAGCG AGCAGAACAG CCTGAAGATA 
GTCAAATCCT CCAGCACGAG TGACCTGTCC TTTATAGCGG GCAATCAATC GAAGGATGTT 
TCCGGCATGC CCGATCCCCT GGATGCCAAA TCGGCCAATC TTGTGAGCAA TTCGTTGCGC 
AAGTCCAAGC TGAAGCACGA TGATCCGGCT TCCCTCTACA AGGGTCCTGG TGGTGCCGAG 
CTGCCCAAGG AGGTACTGGC GGACCTTACC CAATTCACGA GAAAACGCAG CCACGCCGTC 
ATCGATGTCT GGTGGTTGTA CGATGACGGA GGACTCACTC TCCTCTTGCC CTACATCATC 
AGTACGCGGC GCACCTGGCA ATCCTGCAAA TTGAGAGTTT ACGCGCTGGC TAACAAAAAT 
TCGGAATTGG AGTTCGAGCA GCGCTCAATG GCCAGTTTGC TGTCGAAATT CCGGATCGAT 
TACTCGGATT TAACACTGAT TCCGGATATA ACGAAGAAGC CCCAGGAAAC ATCCACGCAG 
TTCTTTAATG AGCTGATTAA GGACTTTGTT GTTACCGAAA AGGATGGCGA GAATGGCACC 
AGCAGCAGGG CAACTCTCAA CGAGGACGAT GCCCTCATAA CCGACGATGA TCTGTTGGCG 
GTGCAGGACA AGACGAATCG GTATTTGCGG CTGCGGGAGT ACCTGCGGGA GCAGTCGACC 
AAGTCGGACC TCGTGGTGAT GACCCTGCCG ATGCCACGGA AGAACATCGT CTCCGCACCC 
CTCTACATGG CCTGGCTGGA GAGCCTGAGT CGGGACATGC CGCCCTTCCT TTTTGTGCGC 
GGCAATCAGA CGAGTGTGCT GACCTTCTAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncc69 ( NM_001274828.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261757.1 Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant D, mRNA.

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CloneID	OFa20833
Clone ID Related Accession (Same CDS sequence)	NM_001274830.2 , NM_001274827.1 , NM_001274828.1
Accession Version	NM_001274828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sodium chloride cotransporter 69, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGAAG ACCGAAACCG CAACCAGTTG GTCCACAGCG AGCAGAACAG CCTGAAGATA 
GTCAAATCCT CCAGCACGAG TGACCTGTCC TTTATAGCGG GCAATCAATC GAAGGATGTT 
TCCGGCATGC CCGATCCCCT GGATGCCAAA TCGGCCAATC TTGTGAGCAA TTCGTTGCGC 
AAGTCCAAGC TGAAGCACGA TGATCCGGCT TCCCTCTACA AGGGTCCTGG TGGTGCCGAG 
CTGCCCAAGG AGGTACTGGC GGACCTTACC CAATTCACGA GAAAACGCAG CCACGCCGTC 
ATCGATGTCT GGTGGTTGTA CGATGACGGA GGACTCACTC TCCTCTTGCC CTACATCATC 
AGTACGCGGC GCACCTGGCA ATCCTGCAAA TTGAGAGTTT ACGCGCTGGC TAACAAAAAT 
TCGGAATTGG AGTTCGAGCA GCGCTCAATG GCCAGTTTGC TGTCGAAATT CCGGATCGAT 
TACTCGGATT TAACACTGAT TCCGGATATA ACGAAGAAGC CCCAGGAAAC ATCCACGCAG 
TTCTTTAATG AGCTGATTAA GGACTTTGTT GTTACCGAAA AGGATGGCGA GAATGGCACC 
AGCAGCAGGG CAACTCTCAA CGAGGACGAT GCCCTCATAA CCGACGATGA TCTGTTGGCG 
GTGCAGGACA AGACGAATCG GTATTTGCGG CTGCGGGAGT ACCTGCGGGA GCAGTCGACC 
AAGTCGGACC TCGTGGTGAT GACCCTGCCG ATGCCACGGA AGAACATCGT CTCCGCACCC 
CTCTACATGG CCTGGCTGGA GAGCCTGAGT CGGGACATGC CGCCCTTCCT TTTTGTGCGC 
GGCAATCAGA CGAGTGTGCT GACCTTCTAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261757.1
CDS	354..3929
Translation	MSDTISFELGSAADRPPNRFQVNPVNGNSRKSQGADGPGSGSGAGAGAGAGAGEDGPHEVYRRLTNAEGELLEDDTFDATQMLNQRQPRQQRQSIKSSFRDKDKPSRFKDLQTTTRFQVDPQNEESDESNDSQEERELLDNEYDTKYGKSFRHFTREALPRLDNYRNMMSIQAAYRPTLDELHNATLVGKNTHSLTRNQDPESGILNGVLKFGWIKGVLVRCLLNIWGVMLFLRLSWVVGQAGVIEGFVLILTTTAVTTITALSMSAISTNGVIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACAMYVVGFCESMLAMMTTFGWEIIDGGVQDVRIIGCITILLLLIIVVVGMEWEAKAQIGLLIILLVAIGDFVIGSFIGPKSDAEMAKGFLGYNATLFKNNLFADYRPEKGGIQHDFFSVFAIFFPAATGILAGANISGDLKDPQKSIPKGTILAIVITTGTYLIMVLQCGATVARDATGNLSDVVNGTFAFLDCQPGECNYGLQNSFQVIELVSAFGPLIYAGCYAATLSSALASLVSAPKVFQALCKDELYPKIVWFAKGYGKNNEPVRGYVLTFIIACAFILIGELNLIAPLISNFFLAAYMLINFSTFHASLAKPVGWRPTFKYYNMWLSLLGAILCVAVMFLISWATALITFAAVLALYLIVAYRKPDVNWGSTTQAQTYKNALMSVQQLNNVEEHVKNYRPQILVLSGLPNTRPVLVDLAYMLTKNLSLLVCGHVLKGSSSQKYRTYLQERAGNWFRKHRVKGFYALVDGEDFESGTRALMQATGIGKLKPNIILMGYKTDWQTCDHKELDQYFNVMHKALDMYLSVAILRVPQGLDCSQVLGSQDGWKTVSDVPRTLQPNESSGDLQAVDSSVRNGLSGSIDSLSRNVSQEDRNRNQLVHSEQNSLKIVKSSSTSDLSFIAGNQSKDVSGMPDPLDAKSANLVSNSLRKSKLKHDDPASLYKGPGGAELPKEVLADLTQFTRKRSHAVIDVWWLYDDGGLTLLLPYIISTRRTWQSCKLRVYALANKNSELEFEQRSMASLLSKFRIDYSDLTLIPDITKKPQETSTQFFNELIKDFVVTEKDGENGTSSRATLNEDDALITDDDLLAVQDKTNRYLRLREYLREQSTKSDLVVMTLPMPRKNIVSAPLYMAWLESLSRDMPPFLFVRGNQTSVLTFYS

Target ORF information:

RefSeq Version	NM_001274828.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274828.1

ORF Insert Sequence:

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGAAG ACCGAAACCG CAACCAGTTG GTCCACAGCG AGCAGAACAG CCTGAAGATA 
GTCAAATCCT CCAGCACGAG TGACCTGTCC TTTATAGCGG GCAATCAATC GAAGGATGTT 
TCCGGCATGC CCGATCCCCT GGATGCCAAA TCGGCCAATC TTGTGAGCAA TTCGTTGCGC 
AAGTCCAAGC TGAAGCACGA TGATCCGGCT TCCCTCTACA AGGGTCCTGG TGGTGCCGAG 
CTGCCCAAGG AGGTACTGGC GGACCTTACC CAATTCACGA GAAAACGCAG CCACGCCGTC 
ATCGATGTCT GGTGGTTGTA CGATGACGGA GGACTCACTC TCCTCTTGCC CTACATCATC 
AGTACGCGGC GCACCTGGCA ATCCTGCAAA TTGAGAGTTT ACGCGCTGGC TAACAAAAAT 
TCGGAATTGG AGTTCGAGCA GCGCTCAATG GCCAGTTTGC TGTCGAAATT CCGGATCGAT 
TACTCGGATT TAACACTGAT TCCGGATATA ACGAAGAAGC CCCAGGAAAC ATCCACGCAG 
TTCTTTAATG AGCTGATTAA GGACTTTGTT GTTACCGAAA AGGATGGCGA GAATGGCACC 
AGCAGCAGGG CAACTCTCAA CGAGGACGAT GCCCTCATAA CCGACGATGA TCTGTTGGCG 
GTGCAGGACA AGACGAATCG GTATTTGCGG CTGCGGGAGT ACCTGCGGGA GCAGTCGACC 
AAGTCGGACC TCGTGGTGAT GACCCTGCCG ATGCCACGGA AGAACATCGT CTCCGCACCC 
CTCTACATGG CCTGGCTGGA GAGCCTGAGT CGGGACATGC CGCCCTTCCT TTTTGTGCGC 
GGCAATCAGA CGAGTGTGCT GACCTTCTAC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncc69 ( NM_001274829.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261758.1 Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant E, mRNA.

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CloneID	OFa20834
Clone ID Related Accession (Same CDS sequence)	NM_001274829.1
Accession Version	NM_001274829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sodium chloride cotransporter 69, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGAAG ACCGAAACCG CAACCAGTTG GTCCACAGCG AGCAGAACAG CCTGAAGATA 
GTCAAATTGC GATTCAAGCA GGGCCTGCGT AGAATGCGCT CGTGGCCAGG TGCAGCCTCC 
AGCACGAGTG ACCTGTCCTT TATAGCGGGC AATCAATCGA AGGATGTTTC CGGCATGCCC 
GATCCCCTGG ATGCCAAATC GGCCAATCTT GTGAGCAATT CGTTGCGCAA GTCCAAGCTG 
AAGCACGATG ATCCGGCTTC CCTCTACAAG GGTCCTGGTG GTGCCGAGCT GCCCAAGGAG 
GTACTGGCGG ACCTTACCCA ATTCACGAGA AAACGCAGCC ACGCCGTCAT CGATGTCTGG 
TGGTTGTACG ATGACGGAGG ACTCACTCTC CTCTTGCCCT ACATCATCAG TACGCGGCGC 
ACCTGGCAAT CCTGCAAATT GAGAGTTTAC GCGCTGGCTA ACAAAAATTC GGAATTGGAG 
TTCGAGCAGC GCTCAATGGC CAGTTTGCTG TCGAAATTCC GGATCGATTA CTCGGATTTA 
ACACTGATTC CGGATATAAC GAAGAAGCCC CAGGAAACAT CCACGCAGTT CTTTAATGAG 
CTGATTAAGG ACTTTGTTGT TACCGAAAAG GATGGCGAGA ATGGCACCAG CAGCAGGGCA 
ACTCTCAACG AGGACGATGC CCTCATAACC GACGATGATC TGTTGGCGGT GCAGGACAAG 
ACGAATCGGT ATTTGCGGCT GCGGGAGTAC CTGCGGGAGC AGTCGACCAA GTCGGACCTC 
GTGGTGATGA CCCTGCCGAT GCCACGGAAG AACATCGTCT CCGCACCCCT CTACATGGCC 
TGGCTGGAGA GCCTGAGTCG GGACATGCCG CCCTTCCTTT TTGTGCGCGG CAATCAGACG 
AGTGTGCTGA CCTTCTACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261758.1
CDS	354..3977
Translation	MSDTISFELGSAADRPPNRFQVNPVNGNSRKSQGADGPGSGSGAGAGAGAGAGEDGPHEVYRRLTNAEGELLEDDTFDATQMLNQRQPRQQRQSIKSSFRDKDKPSRFKDLQTTTRFQVDPQNEESDESNDSQEERELLDNEYDTKYGKSFRHFTREALPRLDNYRNMMSIQAAYRPTLDELHNATLVGKNTHSLTRNQDPESGILNGVLKFGWIKGVLVRCLLNIWGVMLFLRLSWVVGQAGVIEGFVLILTTTAVTTITALSMSAISTNGVIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACAMYVVGFCESMLAMMTTFGWEIIDGGVQDVRIIGCITILLLLIIVVVGMEWEAKAQIGLLIILLVAIGDFVIGSFIGPKSDAEMAKGFLGYNATLFKNNLFADYRPEKGGIQHDFFSVFAIFFPAATGILAGANISGDLKDPQKSIPKGTILAIVITTGTYLIMVLQCGATVARDATGNLSDVVNGTFAFLDCQPGECNYGLQNSFQVIELVSAFGPLIYAGCYAATLSSALASLVSAPKVFQALCKDELYPKIVWFAKGYGKNNEPVRGYVLTFIIACAFILIGELNLIAPLISNFFLAAYMLINFSTFHASLAKPVGWRPTFKYYNMWLSLLGAILCVAVMFLISWATALITFAAVLALYLIVAYRKPDVNWGSTTQAQTYKNALMSVQQLNNVEEHVKNYRPQILVLSGLPNTRPVLVDLAYMLTKNLSLLVCGHVLKGSSSQKYRTYLQERAGNWFRKHRVKGFYALVDGEDFESGTRALMQATGIGKLKPNIILMGYKTDWQTCDHKELDQYFNVMHKALDMYLSVAILRVPQGLDCSQVLGSQDGWKTVSDVPRTLQPNESSGDLQAVDSSVRNGLSGSIDSLSRNVSQEDRNRNQLVHSEQNSLKIVKLRFKQGLRRMRSWPGAASSTSDLSFIAGNQSKDVSGMPDPLDAKSANLVSNSLRKSKLKHDDPASLYKGPGGAELPKEVLADLTQFTRKRSHAVIDVWWLYDDGGLTLLLPYIISTRRTWQSCKLRVYALANKNSELEFEQRSMASLLSKFRIDYSDLTLIPDITKKPQETSTQFFNELIKDFVVTEKDGENGTSSRATLNEDDALITDDDLLAVQDKTNRYLRLREYLREQSTKSDLVVMTLPMPRKNIVSAPLYMAWLESLSRDMPPFLFVRGNQTSVLTFYS

Target ORF information:

RefSeq Version	NM_001274829.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sodium chloride cotransporter 69 (Ncc69), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274829.1

ORF Insert Sequence:

ATGTCGGACA CAATCTCTTT CGAGTTGGGC TCAGCTGCCG ACCGGCCCCC CAACAGGTTT 
CAAGTGAACC CGGTCAACGG CAACAGTCGT AAGTCGCAGG GAGCGGATGG CCCAGGATCC 
GGATCAGGAG CGGGAGCTGG AGCAGGAGCA GGAGCTGGGG AGGATGGGCC GCACGAGGTC 
TACCGCCGAC TAACAAACGC CGAGGGCGAG CTGCTCGAGG ACGACACATT CGATGCGACA 
CAAATGCTCA ACCAACGACA GCCCAGGCAG CAGAGGCAAT CAATCAAAAG CAGTTTCCGC 
GACAAGGATA AACCGTCAAG GTTCAAGGAT CTGCAAACGA CGACCCGCTT CCAGGTGGAT 
CCCCAAAATG AGGAGTCCGA CGAGTCCAAT GACTCGCAGG AGGAGCGCGA GCTGCTGGAC 
AACGAGTACG ACACAAAATA TGGTAAAAGT TTCCGGCATT TCACCAGAGA GGCGTTACCC 
CGCCTGGACA ACTACCGCAA CATGATGTCC ATCCAGGCGG CCTACCGTCC AACGCTCGAC 
GAGCTGCATA ACGCCACGCT GGTGGGCAAG AACACGCACA GCTTGACACG TAATCAGGAC 
CCGGAGTCGG GCATCCTGAA TGGGGTCCTG AAATTCGGTT GGATCAAAGG TGTGCTCGTC 
CGCTGCCTGC TGAACATCTG GGGCGTGATG CTGTTCCTCC GGCTCAGCTG GGTGGTGGGT 
CAGGCGGGCG TCATCGAGGG ATTCGTATTA ATTCTGACAA CGACTGCTGT CACGACCATC 
ACGGCCTTGT CGATGTCGGC GATAAGCACT AATGGTGTCA TCAAAGGGGG TGGCACATAC 
TATATGATAT CCCGGTCTCT GGGGCCGGAG TTTGGTGGAT CCATCGGTTT GATATTCTCC 
CTGGCAAATG CGGTGGCGTG TGCCATGTAT GTGGTCGGCT TCTGCGAGTC TATGTTGGCC 
ATGATGACGA CCTTTGGCTG GGAGATCATA GATGGTGGCG TCCAGGATGT GCGCATCATT 
GGTTGCATTA CCATACTGCT GCTCCTCATC ATTGTGGTCG TCGGCATGGA GTGGGAGGCC 
AAGGCGCAAA TCGGACTGCT CATCATCCTG TTGGTGGCCA TTGGAGACTT TGTTATTGGC 
AGCTTTATCG GACCGAAGAG CGATGCGGAA ATGGCCAAGG GATTTTTGGG ATATAATGCC 
ACTTTGTTTA AGAACAACCT TTTCGCGGAC TATCGGCCGG AAAAGGGAGG CATTCAACAC 
GATTTTTTCT CAGTGTTTGC TATATTCTTC CCTGCCGCAA CGGGCATTTT AGCTGGAGCC 
AATATTTCGG GCGATCTGAA GGACCCACAA AAATCCATTC CAAAAGGCAC GATCCTGGCC 
ATCGTTATCA CCACCGGAAC CTATTTGATT ATGGTACTCC AGTGTGGTGC CACAGTGGCT 
CGTGATGCCA CGGGAAATCT TTCAGATGTC GTAAACGGCA CCTTTGCATT CCTCGACTGT 
CAGCCTGGTG AATGCAATTA CGGCTTGCAA AACTCATTTC AAGTAATTGA GTTGGTATCC 
GCGTTTGGCC CTCTGATTTA CGCCGGTTGC TATGCTGCCA CGTTATCCTC GGCATTGGCC 
AGTTTGGTGT CTGCCCCGAA GGTCTTCCAG GCTCTGTGCA AGGATGAGCT GTATCCGAAG 
ATTGTGTGGT TTGCCAAAGG TTATGGCAAG AATAATGAGC CAGTTCGTGG CTATGTGTTA 
ACTTTCATCA TCGCCTGTGC CTTCATTTTG ATTGGCGAAC TGAACCTGAT TGCCCCGCTC 
ATTTCGAACT TCTTCCTGGC CGCCTACATG TTGATCAACT TCAGTACCTT CCATGCCAGT 
CTGGCCAAGC CAGTGGGCTG GCGACCGACC TTTAAGTATT ACAATATGTG GCTGAGCCTG 
TTGGGCGCCA TCCTCTGTGT GGCCGTCATG TTCCTCATCT CGTGGGCCAC AGCGCTCATC 
ACCTTTGCCG CCGTGCTGGC TCTGTACTTA ATTGTGGCCT ACCGGAAACC GGATGTCAAC 
TGGGGCTCCA CCACCCAGGC TCAGACGTAC AAGAACGCCC TGATGTCGGT GCAGCAGCTG 
AACAATGTGG AGGAGCACGT GAAGAACTAC CGGCCACAGA TCCTGGTTCT TTCCGGTTTG 
CCCAACACAC GGCCGGTGCT CGTCGACTTG GCTTACATGC TAACCAAGAA TTTATCCCTG 
CTCGTCTGTG GTCACGTTCT GAAAGGTTCC AGCTCGCAGA AGTACCGGAC ATATCTGCAG 
GAAAGGGCGG GCAATTGGTT CCGGAAGCAT CGCGTTAAGG GCTTCTATGC CCTGGTGGAT 
GGTGAGGACT TTGAGTCCGG CACTCGGGCT CTGATGCAAG CAACTGGTAT TGGAAAACTT 
AAGCCGAACA TCATCCTGAT GGGCTACAAG ACTGACTGGC AGACGTGCGA TCACAAGGAG 
CTGGATCAGT ACTTCAATGT GATGCACAAG GCACTGGACA TGTACCTATC CGTGGCCATT 
TTGCGTGTGC CCCAGGGTCT GGACTGTTCG CAGGTGCTGG GCTCGCAGGA TGGTTGGAAG 
ACCGTTTCGG ATGTGCCGAG AACCCTGCAG CCAAACGAGA GTTCCGGGGA TCTGCAGGCG 
GTGGACAGTA GTGTCAGGAA CGGTTTAAGT GGCAGCATTG ATTCACTCAG CAGAAATGTA 
TCGCAAGAAG ACCGAAACCG CAACCAGTTG GTCCACAGCG AGCAGAACAG CCTGAAGATA 
GTCAAATTGC GATTCAAGCA GGGCCTGCGT AGAATGCGCT CGTGGCCAGG TGCAGCCTCC 
AGCACGAGTG ACCTGTCCTT TATAGCGGGC AATCAATCGA AGGATGTTTC CGGCATGCCC 
GATCCCCTGG ATGCCAAATC GGCCAATCTT GTGAGCAATT CGTTGCGCAA GTCCAAGCTG 
AAGCACGATG ATCCGGCTTC CCTCTACAAG GGTCCTGGTG GTGCCGAGCT GCCCAAGGAG 
GTACTGGCGG ACCTTACCCA ATTCACGAGA AAACGCAGCC ACGCCGTCAT CGATGTCTGG 
TGGTTGTACG ATGACGGAGG ACTCACTCTC CTCTTGCCCT ACATCATCAG TACGCGGCGC 
ACCTGGCAAT CCTGCAAATT GAGAGTTTAC GCGCTGGCTA ACAAAAATTC GGAATTGGAG 
TTCGAGCAGC GCTCAATGGC CAGTTTGCTG TCGAAATTCC GGATCGATTA CTCGGATTTA 
ACACTGATTC CGGATATAAC GAAGAAGCCC CAGGAAACAT CCACGCAGTT CTTTAATGAG 
CTGATTAAGG ACTTTGTTGT TACCGAAAAG GATGGCGAGA ATGGCACCAG CAGCAGGGCA 
ACTCTCAACG AGGACGATGC CCTCATAACC GACGATGATC TGTTGGCGGT GCAGGACAAG 
ACGAATCGGT ATTTGCGGCT GCGGGAGTAC CTGCGGGAGC AGTCGACCAA GTCGGACCTC 
GTGGTGATGA CCCTGCCGAT GCCACGGAAG AACATCGTCT CCGCACCCCT CTACATGGCC 
TGGCTGGAGA GCCTGAGTCG GGACATGCCG CCCTTCCTTT TTGTGCGCGG CAATCAGACG 
AGTGTGCTGA CCTTCTACTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ncc69 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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