Vha14-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vha14-2
Entrez Gene ID	40748
Full Name	Vacuolar H[+] ATPase 14kD subunit 2
Synonyms	CG1076,Dmel\CG1076,vha14-2
General protein information	Preferred Names Vacuolar H[+] ATPase 14kD subunit 2 Names CG1076 gene product from transcript CG1076-RC CG1076-PA CG1076-PB CG1076-PC Vha14-2-PA Vha14-2-PB Vha14-2-PC vacuolar H[+] ATPase 14kD subunit 2 vacuolar H[+] ATPase subunit 14-2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83D4-83D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260015.1	NP_001246944.1	vacuolar H[+] ATPase 14kD subunit 2, isoform C
NM_001260014.1	NP_001246943.1	vacuolar H[+] ATPase 14kD subunit 2, isoform B
NM_141357.2	NP_649614.1	vacuolar H[+] ATPase 14kD subunit 2, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vha14-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vha14-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04037	NM_141357.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 14kD subunit 2, transcript variant A (Vha14-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa07897	NM_001260015.1 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 14kD subunit 2, transcript variant C (Vha14-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa07897	NM_001260014.1 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 14kD subunit 2, transcript variant B (Vha14-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vha14-2 ( NM_141357.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649614.1 Drosophila melanogaster vacuolar H[+] ATPase 14kD subunit 2, transcript variant A (Vha14-2), mRNA.

Price & Availability↑

CloneID	OFa04037
Clone ID Related Accession (Same CDS sequence)	NM_141357.2
Accession Version	NM_141357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 14kD subunit 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_141357.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCACAC CCACACACAA TTTACCCAGG ACACGTGTGT CGGCTTTCTG CTGGGTGGCA 
TTGGAGAGGT CGACGAGGAT CGAGAGACCA ACTTCATGGT CGTGGAAAGA GTTATCGCAT 
TATTCTCTTA TCGTTTTTCA TTGTATACCA GACACCACCC CTAAGCAGAT CGAAGAGTGT 
TTCAAAAAGT TCCTGAGGCG ACCGGATATA GTCATTATTC TGATCAACCA GGTTTACGCT 
GATATGATTC GACCCACTGT CGATGCTCAC AATCTGGCCG TTCCCACTGT GCTGGAGATT 
CCCTCGAAAC AGCATCCCTA CGATTCCTCC AGGGACTCGA TTCTGAAGCG GGCTCAACGA 
GTCATCACTC CGCCCGAGAG GCACTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649614.1
CDS	153..542
Translation	MSTPTHNLPRTRVSAFCWVALERSTRIERPTSWSWKELSHYSLIVFHCIPDTTPKQIEECFKKFLRRPDIVIILINQVYADMIRPTVDAHNLAVPTVLEIPSKQHPYDSSRDSILKRAQRVITPPERHY

Target ORF information:

RefSeq Version	NM_141357.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 14kD subunit 2, transcript variant A (Vha14-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141357.2

ORF Insert Sequence:

ATGTCCACAC CCACACACAA TTTACCCAGG ACACGTGTGT CGGCTTTCTG CTGGGTGGCA 
TTGGAGAGGT CGACGAGGAT CGAGAGACCA ACTTCATGGT CGTGGAAAGA GTTATCGCAT 
TATTCTCTTA TCGTTTTTCA TTGTATACCA GACACCACCC CTAAGCAGAT CGAAGAGTGT 
TTCAAAAAGT TCCTGAGGCG ACCGGATATA GTCATTATTC TGATCAACCA GGTTTACGCT 
GATATGATTC GACCCACTGT CGATGCTCAC AATCTGGCCG TTCCCACTGT GCTGGAGATT 
CCCTCGAAAC AGCATCCCTA CGATTCCTCC AGGGACTCGA TTCTGAAGCG GGCTCAACGA 
GTCATCACTC CGCCCGAGAG GCACTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha14-2 ( NM_001260015.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246944.1 Drosophila melanogaster vacuolar H[+] ATPase 14kD subunit 2, transcript variant C (Vha14-2), mRNA.

Price & Availability↑

CloneID	OFa07897
Clone ID Related Accession (Same CDS sequence)	NM_001260015.1 , NM_001260014.1
Accession Version	NM_001260015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 14kD subunit 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGC ACACAGAGGA TCTGGGACGC CTTTTGGCCG TGATTGGCGA TGAGGACACG 
TGTGTCGGCT TTCTGCTGGG TGGCATTGGA GAGGTCGACG AGGATCGAGA GACCAACTTC 
ATGGTCGTGG AAAGAGACAC CACCCCTAAG CAGATCGAAG AGTGTTTCAA AAAGTTCCTG 
AGGCGACCGG ATATAGTCAT TATTCTGATC AACCAGGTTT ACGCTGATAT GATTCGACCC 
ACTGTCGATG CTCACAATCT GGCCGTTCCC ACTGTGCTGG AGATTCCCTC GAAACAGCAT 
CCCTACGATT CCTCCAGGGA CTCGATTCTG AAGCGGGCTC AACGAGTCAT CACTCCGCCC 
GAGAGGCACT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246944.1
CDS	66..440
Translation	MSSHTEDLGRLLAVIGDEDTCVGFLLGGIGEVDEDRETNFMVVERDTTPKQIEECFKKFLRRPDIVIILINQVYADMIRPTVDAHNLAVPTVLEIPSKQHPYDSSRDSILKRAQRVITPPERHY

Target ORF information:

RefSeq Version	NM_001260015.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 14kD subunit 2, transcript variant C (Vha14-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260015.1

ORF Insert Sequence:

ATGTCGTCGC ACACAGAGGA TCTGGGACGC CTTTTGGCCG TGATTGGCGA TGAGGACACG 
TGTGTCGGCT TTCTGCTGGG TGGCATTGGA GAGGTCGACG AGGATCGAGA GACCAACTTC 
ATGGTCGTGG AAAGAGACAC CACCCCTAAG CAGATCGAAG AGTGTTTCAA AAAGTTCCTG 
AGGCGACCGG ATATAGTCAT TATTCTGATC AACCAGGTTT ACGCTGATAT GATTCGACCC 
ACTGTCGATG CTCACAATCT GGCCGTTCCC ACTGTGCTGG AGATTCCCTC GAAACAGCAT 
CCCTACGATT CCTCCAGGGA CTCGATTCTG AAGCGGGCTC AACGAGTCAT CACTCCGCCC 
GAGAGGCACT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha14-2 ( NM_001260014.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246943.1 Drosophila melanogaster vacuolar H[+] ATPase 14kD subunit 2, transcript variant B (Vha14-2), mRNA.

Price & Availability↑

CloneID	OFa07897
Clone ID Related Accession (Same CDS sequence)	NM_001260015.1 , NM_001260014.1
Accession Version	NM_001260014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 14kD subunit 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGC ACACAGAGGA TCTGGGACGC CTTTTGGCCG TGATTGGCGA TGAGGACACG 
TGTGTCGGCT TTCTGCTGGG TGGCATTGGA GAGGTCGACG AGGATCGAGA GACCAACTTC 
ATGGTCGTGG AAAGAGACAC CACCCCTAAG CAGATCGAAG AGTGTTTCAA AAAGTTCCTG 
AGGCGACCGG ATATAGTCAT TATTCTGATC AACCAGGTTT ACGCTGATAT GATTCGACCC 
ACTGTCGATG CTCACAATCT GGCCGTTCCC ACTGTGCTGG AGATTCCCTC GAAACAGCAT 
CCCTACGATT CCTCCAGGGA CTCGATTCTG AAGCGGGCTC AACGAGTCAT CACTCCGCCC 
GAGAGGCACT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246943.1
CDS	66..440
Translation	MSSHTEDLGRLLAVIGDEDTCVGFLLGGIGEVDEDRETNFMVVERDTTPKQIEECFKKFLRRPDIVIILINQVYADMIRPTVDAHNLAVPTVLEIPSKQHPYDSSRDSILKRAQRVITPPERHY

Target ORF information:

RefSeq Version	NM_001260014.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 14kD subunit 2, transcript variant B (Vha14-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260014.1

ORF Insert Sequence:

ATGTCGTCGC ACACAGAGGA TCTGGGACGC CTTTTGGCCG TGATTGGCGA TGAGGACACG 
TGTGTCGGCT TTCTGCTGGG TGGCATTGGA GAGGTCGACG AGGATCGAGA GACCAACTTC 
ATGGTCGTGG AAAGAGACAC CACCCCTAAG CAGATCGAAG AGTGTTTCAA AAAGTTCCTG 
AGGCGACCGG ATATAGTCAT TATTCTGATC AACCAGGTTT ACGCTGATAT GATTCGACCC 
ACTGTCGATG CTCACAATCT GGCCGTTCCC ACTGTGCTGG AGATTCCCTC GAAACAGCAT 
CCCTACGATT CCTCCAGGGA CTCGATTCTG AAGCGGGCTC AACGAGTCAT CACTCCGCCC 
GAGAGGCACT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vha14-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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