JHDM2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	JHDM2
Entrez Gene ID	41143
Full Name	JmjC domain-containing histone demethylase 2
Synonyms	CG31123,CG8165,Dmel\CG8165
General protein information	Preferred Names JmjC domain-containing histone demethylase 2 Names CG8165 gene product from transcript CG8165-RA CG8165-PA CG8165-PB JHDM2-PA JHDM2-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85D19-85D19

mRNA and Protein(s)

mRNA	Protein	Name
NM_176434.2	NP_788611.1	JmjC domain-containing histone demethylase 2, isoform A
NM_001300326.1	NP_001287255.1	JmjC domain-containing histone demethylase 2, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	JHDM2	NP_788611.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002682	XP_003436208.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Paper describes the multifunctional cupin superfamily. This protein contains the cupin signature at its C-terminus. Residues H643, D645, and H774 represent the conserved Fe binding ligands of the active site of this presumed dioxygenase subtype.
Title: Evolution of functional diversity in the cupin superfamily.

The following JHDM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the JHDM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08219	NM_001300326.1 Latest version!	Drosophila melanogaster JmjC domain-containing histone demethylase 2 (JHDM2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08219	NM_176434.2 Latest version!	Drosophila melanogaster JmjC domain-containing histone demethylase 2 (JHDM2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

JHDM2 ( NM_176434.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788611.1 Drosophila melanogaster JmjC domain-containing histone demethylase 2 (JHDM2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa08219
Clone ID Related Accession (Same CDS sequence)	NM_176434.2 , NM_001300326.1
Accession Version	NM_176434.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	JmjC domain-containing histone demethylase 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176434.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCAAA AAGAATTGGC GGCTCTGGTT GCCACGAGAA CGAGTCGCAA ACGCACCATA 
TCCTCGACCT CATTCTCTAT TTCCACTCGC ACTGATCCAA AATCCATCAT AGAGATACAC 
ATATTCAATG ACTCCTTGCC CATAAAAAGA GCTTGCTGCG AAGTGGAGAA TCCAACTTTA 
AAAGCGGAAC AGCGAGTGCA GCAGGAAGAA GAGAGCCTGG GCCAAGTGCC GCCGCTGACC 
GAGGAAGAGC AGCAGCGGCA CGATGAGTTC CGAAATAGTG GTTCGATATT CCTGCAGGAT 
TTGCCCTGCT TTAAGCTGCA GCAGGCACAG CCGATAGCAG GATCATCACC CATTCCCAAG 
TGTCGGGAGT GCAGAAGAAG AAATTTGGCT ACAACAGAGG GCGAACCCAG TTCAGTTAGC 
GACGTTTACT GCCGGTTCTA CGAGTTCCGA CGCCTTCAGT TCAATGAGAA TGGAGAGCTG 
TGCGTGGTTG GCTTCCCAAA TCCCTACAGC GAGCCCTCAC CAGAGGACAT CGCCATTTGG 
CAGCCGGATA AAAATACACC GCCGACCAGC GGTTACATGG ATATACAGGT TTGCAGGTAC 
ATCCTACTCC ATGCCGGCGA CCAGTTCTGC TATATTTGGC ACCAGGAAGC AGAGGCTTTA 
AGTTTGCATC AGAATACGGA CGGAACCATT GCCTGGAAGA AGGCCGTAAA AGGAACACGA 
GAGATTTGCG ACGTGTGTGA CACCACATTG TTCAACTACC ATTGGACTTG CCGCAAGTGC 
GGCTTTGGAG TGTGTCTGGA CTGCGTTAAA GATCGCAAGG AGGGATTGCG ACTGCGGCGA 
GCGGAGAACG CTGCCCAAAA GGGATGCGAT GAGTACCACT GGCTGCTCTG CAGCGACCCC 
AGTGGCCCAC AGGAGCACGT CCTTACCGAG CTGATGCTAA CACAGATCAT AGCCGGAGAT 
GCCCTCAACG TCTTGGGCAG GCTGCTGCAC GAGGTTCGTA CTTTGTGGCA GGTGCCACAG 
GTCTGCGGCT GCCTTCTGAG CAAGCAAGCG ATTGAAGATG CACAATCAAA GGAAGTGATC 
CAGGATATGA TTAAGGAGTC TCAGCTGAAG CAGCACACCA GCTACTCGTC GCTGGCCTCC 
GAGCAGAAGG TGCACCAGCA ACAGCGCCTC GATCAACTGC ACGCCACGAA GTTGGAGTTT 
GCCAGAGAGC TAGGTGTAGA TTATGTTCCA GGTCGAGTGT GGACCAAGGA AACCTTGGGA 
AAGGATCCAA TAACAACGGC GTTTGATAAT TTGAAGCACA TTAATTTTCT GAGAAAGGGA 
TTGGCCGGCT TGAGAAGGTT TCTTCCACCA AGGACCATGA CTTTTGCTTA CTCCACTCAG 
TTGGCTCCAG GAGTTCCTCA CGAGTTTCTG TGCGATGGCA GACTGCTTAG ACTCACCGAT 
GCCATGCATC CGGATAATCG AGTTCTTTTC CAGGAGGTTT GGAAATGCGG CCAGCCAGTT 
ATGATTTCGG AGGTGGCTCG CTCTTTGAAC TTAGACTTAT GGCATCCACA AGCCTTTTGC 
CGGGACTTTG GCGACAAGCC CAATGATCTT ATTAATTGCC TGAATGGCAA TCTGGTGCCC 
AATCAGCCAA TGCGACACTT TTGGGAGGGT TTTCAGTGCA TGACCAAGCG TCTGCCAGAT 
GCATATGGCA AGCCAATGCT CTTAAAGCTT AAGGATTGGC CGCCGGGTGA TGATTTTGCC 
GAAATACTGC CCACCAGGTT TGCTGATTTG ATGAAGGGAC TGCCCATGCC GGAGTACACA 
CTTCGCACGG GAAACCTTAA CATAGCTAGC TGCTTACCCA AGATGTTTGT GCCACCAGAC 
TTAGGACCCA AAATGTACAA CGCATATGGC TCAGCATTGC ACCCCGACAA GGGTACAACC 
AATCTTCACT TGGACATTTC GGATGCAGTG AACATAATGG TTTATGTGGG AATACCCCAG 
GATGGGGACA CGAGACCTCA GATGGCGGCC ACGCAGAAAG CCATAGAAAT TGGCGGATGC 
GATTACATCA CTAGAGCTCG TTGCCAATCG CCAGATGTAT TACCCGGAGC ACTTTGGCAC 
ATCTTTCCAG CTCGCGATGC AGACAAAATA AGAGATTTAC TTAACCGCGT GACCCTGGAG 
AAGGGTTTCC GTCTGGAGCC GGATCACGAT CCAATTCACG ATCAGAATTG GTACTTGGAC 
GACAAGTTGC GAGCTCGTTT ATTCAAAGAG TATGGCGTGG AGGGGCATCC GATTGTCCAG 
TGCTTGGGCG ACGCAGTCTT TATACCAGCT GGCGCCCCAC ATCAGGTTCA AAATCTCCAC 
AACTGTATTA AGGTGGCCGA GGATTTTGTA TCGCCGGAAA ATATCACGCA CTGCTATCAT 
CTAACTCACG AGTTTAGAAG GCTTTCGCAC TCGCACACAA ATCACGAGGA CAAGCTTCAG 
ATCAAGAATA TTATCTACCA TGCCATCAAG GACTGTTGCA CCATCCTGAC CAGAGCTGTG 
GATGAGAGAC TTAATGCGGA ATTAACCAAG CTAAATGCAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788611.1
CDS	176..2740
Translation	MSQKELAALVATRTSRKRTISSTSFSISTRTDPKSIIEIHIFNDSLPIKRACCEVENPTLKAEQRVQQEEESLGQVPPLTEEEQQRHDEFRNSGSIFLQDLPCFKLQQAQPIAGSSPIPKCRECRRRNLATTEGEPSSVSDVYCRFYEFRRLQFNENGELCVVGFPNPYSEPSPEDIAIWQPDKNTPPTSGYMDIQVCRYILLHAGDQFCYIWHQEAEALSLHQNTDGTIAWKKAVKGTREICDVCDTTLFNYHWTCRKCGFGVCLDCVKDRKEGLRLRRAENAAQKGCDEYHWLLCSDPSGPQEHVLTELMLTQIIAGDALNVLGRLLHEVRTLWQVPQVCGCLLSKQAIEDAQSKEVIQDMIKESQLKQHTSYSSLASEQKVHQQQRLDQLHATKLEFARELGVDYVPGRVWTKETLGKDPITTAFDNLKHINFLRKGLAGLRRFLPPRTMTFAYSTQLAPGVPHEFLCDGRLLRLTDAMHPDNRVLFQEVWKCGQPVMISEVARSLNLDLWHPQAFCRDFGDKPNDLINCLNGNLVPNQPMRHFWEGFQCMTKRLPDAYGKPMLLKLKDWPPGDDFAEILPTRFADLMKGLPMPEYTLRTGNLNIASCLPKMFVPPDLGPKMYNAYGSALHPDKGTTNLHLDISDAVNIMVYVGIPQDGDTRPQMAATQKAIEIGGCDYITRARCQSPDVLPGALWHIFPARDADKIRDLLNRVTLEKGFRLEPDHDPIHDQNWYLDDKLRARLFKEYGVEGHPIVQCLGDAVFIPAGAPHQVQNLHNCIKVAEDFVSPENITHCYHLTHEFRRLSHSHTNHEDKLQIKNIIYHAIKDCCTILTRAVDERLNAELTKLNAD

Target ORF information:

RefSeq Version	NM_176434.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster JmjC domain-containing histone demethylase 2 (JHDM2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176434.2

ORF Insert Sequence:

ATGTCGCAAA AAGAATTGGC GGCTCTGGTT GCCACGAGAA CGAGTCGCAA ACGCACCATA 
TCCTCGACCT CATTCTCTAT TTCCACTCGC ACTGATCCAA AATCCATCAT AGAGATACAC 
ATATTCAATG ACTCCTTGCC CATAAAAAGA GCTTGCTGCG AAGTGGAGAA TCCAACTTTA 
AAAGCGGAAC AGCGAGTGCA GCAGGAAGAA GAGAGCCTGG GCCAAGTGCC GCCGCTGACC 
GAGGAAGAGC AGCAGCGGCA CGATGAGTTC CGAAATAGTG GTTCGATATT CCTGCAGGAT 
TTGCCCTGCT TTAAGCTGCA GCAGGCACAG CCGATAGCAG GATCATCACC CATTCCCAAG 
TGTCGGGAGT GCAGAAGAAG AAATTTGGCT ACAACAGAGG GCGAACCCAG TTCAGTTAGC 
GACGTTTACT GCCGGTTCTA CGAGTTCCGA CGCCTTCAGT TCAATGAGAA TGGAGAGCTG 
TGCGTGGTTG GCTTCCCAAA TCCCTACAGC GAGCCCTCAC CAGAGGACAT CGCCATTTGG 
CAGCCGGATA AAAATACACC GCCGACCAGC GGTTACATGG ATATACAGGT TTGCAGGTAC 
ATCCTACTCC ATGCCGGCGA CCAGTTCTGC TATATTTGGC ACCAGGAAGC AGAGGCTTTA 
AGTTTGCATC AGAATACGGA CGGAACCATT GCCTGGAAGA AGGCCGTAAA AGGAACACGA 
GAGATTTGCG ACGTGTGTGA CACCACATTG TTCAACTACC ATTGGACTTG CCGCAAGTGC 
GGCTTTGGAG TGTGTCTGGA CTGCGTTAAA GATCGCAAGG AGGGATTGCG ACTGCGGCGA 
GCGGAGAACG CTGCCCAAAA GGGATGCGAT GAGTACCACT GGCTGCTCTG CAGCGACCCC 
AGTGGCCCAC AGGAGCACGT CCTTACCGAG CTGATGCTAA CACAGATCAT AGCCGGAGAT 
GCCCTCAACG TCTTGGGCAG GCTGCTGCAC GAGGTTCGTA CTTTGTGGCA GGTGCCACAG 
GTCTGCGGCT GCCTTCTGAG CAAGCAAGCG ATTGAAGATG CACAATCAAA GGAAGTGATC 
CAGGATATGA TTAAGGAGTC TCAGCTGAAG CAGCACACCA GCTACTCGTC GCTGGCCTCC 
GAGCAGAAGG TGCACCAGCA ACAGCGCCTC GATCAACTGC ACGCCACGAA GTTGGAGTTT 
GCCAGAGAGC TAGGTGTAGA TTATGTTCCA GGTCGAGTGT GGACCAAGGA AACCTTGGGA 
AAGGATCCAA TAACAACGGC GTTTGATAAT TTGAAGCACA TTAATTTTCT GAGAAAGGGA 
TTGGCCGGCT TGAGAAGGTT TCTTCCACCA AGGACCATGA CTTTTGCTTA CTCCACTCAG 
TTGGCTCCAG GAGTTCCTCA CGAGTTTCTG TGCGATGGCA GACTGCTTAG ACTCACCGAT 
GCCATGCATC CGGATAATCG AGTTCTTTTC CAGGAGGTTT GGAAATGCGG CCAGCCAGTT 
ATGATTTCGG AGGTGGCTCG CTCTTTGAAC TTAGACTTAT GGCATCCACA AGCCTTTTGC 
CGGGACTTTG GCGACAAGCC CAATGATCTT ATTAATTGCC TGAATGGCAA TCTGGTGCCC 
AATCAGCCAA TGCGACACTT TTGGGAGGGT TTTCAGTGCA TGACCAAGCG TCTGCCAGAT 
GCATATGGCA AGCCAATGCT CTTAAAGCTT AAGGATTGGC CGCCGGGTGA TGATTTTGCC 
GAAATACTGC CCACCAGGTT TGCTGATTTG ATGAAGGGAC TGCCCATGCC GGAGTACACA 
CTTCGCACGG GAAACCTTAA CATAGCTAGC TGCTTACCCA AGATGTTTGT GCCACCAGAC 
TTAGGACCCA AAATGTACAA CGCATATGGC TCAGCATTGC ACCCCGACAA GGGTACAACC 
AATCTTCACT TGGACATTTC GGATGCAGTG AACATAATGG TTTATGTGGG AATACCCCAG 
GATGGGGACA CGAGACCTCA GATGGCGGCC ACGCAGAAAG CCATAGAAAT TGGCGGATGC 
GATTACATCA CTAGAGCTCG TTGCCAATCG CCAGATGTAT TACCCGGAGC ACTTTGGCAC 
ATCTTTCCAG CTCGCGATGC AGACAAAATA AGAGATTTAC TTAACCGCGT GACCCTGGAG 
AAGGGTTTCC GTCTGGAGCC GGATCACGAT CCAATTCACG ATCAGAATTG GTACTTGGAC 
GACAAGTTGC GAGCTCGTTT ATTCAAAGAG TATGGCGTGG AGGGGCATCC GATTGTCCAG 
TGCTTGGGCG ACGCAGTCTT TATACCAGCT GGCGCCCCAC ATCAGGTTCA AAATCTCCAC 
AACTGTATTA AGGTGGCCGA GGATTTTGTA TCGCCGGAAA ATATCACGCA CTGCTATCAT 
CTAACTCACG AGTTTAGAAG GCTTTCGCAC TCGCACACAA ATCACGAGGA CAAGCTTCAG 
ATCAAGAATA TTATCTACCA TGCCATCAAG GACTGTTGCA CCATCCTGAC CAGAGCTGTG 
GATGAGAGAC TTAATGCGGA ATTAACCAAG CTAAATGCAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JHDM2 ( NM_001300326.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287255.1 Drosophila melanogaster JmjC domain-containing histone demethylase 2 (JHDM2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08219
Clone ID Related Accession (Same CDS sequence)	NM_176434.2 , NM_001300326.1
Accession Version	NM_001300326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	JmjC domain-containing histone demethylase 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCAAA AAGAATTGGC GGCTCTGGTT GCCACGAGAA CGAGTCGCAA ACGCACCATA 
TCCTCGACCT CATTCTCTAT TTCCACTCGC ACTGATCCAA AATCCATCAT AGAGATACAC 
ATATTCAATG ACTCCTTGCC CATAAAAAGA GCTTGCTGCG AAGTGGAGAA TCCAACTTTA 
AAAGCGGAAC AGCGAGTGCA GCAGGAAGAA GAGAGCCTGG GCCAAGTGCC GCCGCTGACC 
GAGGAAGAGC AGCAGCGGCA CGATGAGTTC CGAAATAGTG GTTCGATATT CCTGCAGGAT 
TTGCCCTGCT TTAAGCTGCA GCAGGCACAG CCGATAGCAG GATCATCACC CATTCCCAAG 
TGTCGGGAGT GCAGAAGAAG AAATTTGGCT ACAACAGAGG GCGAACCCAG TTCAGTTAGC 
GACGTTTACT GCCGGTTCTA CGAGTTCCGA CGCCTTCAGT TCAATGAGAA TGGAGAGCTG 
TGCGTGGTTG GCTTCCCAAA TCCCTACAGC GAGCCCTCAC CAGAGGACAT CGCCATTTGG 
CAGCCGGATA AAAATACACC GCCGACCAGC GGTTACATGG ATATACAGGT TTGCAGGTAC 
ATCCTACTCC ATGCCGGCGA CCAGTTCTGC TATATTTGGC ACCAGGAAGC AGAGGCTTTA 
AGTTTGCATC AGAATACGGA CGGAACCATT GCCTGGAAGA AGGCCGTAAA AGGAACACGA 
GAGATTTGCG ACGTGTGTGA CACCACATTG TTCAACTACC ATTGGACTTG CCGCAAGTGC 
GGCTTTGGAG TGTGTCTGGA CTGCGTTAAA GATCGCAAGG AGGGATTGCG ACTGCGGCGA 
GCGGAGAACG CTGCCCAAAA GGGATGCGAT GAGTACCACT GGCTGCTCTG CAGCGACCCC 
AGTGGCCCAC AGGAGCACGT CCTTACCGAG CTGATGCTAA CACAGATCAT AGCCGGAGAT 
GCCCTCAACG TCTTGGGCAG GCTGCTGCAC GAGGTTCGTA CTTTGTGGCA GGTGCCACAG 
GTCTGCGGCT GCCTTCTGAG CAAGCAAGCG ATTGAAGATG CACAATCAAA GGAAGTGATC 
CAGGATATGA TTAAGGAGTC TCAGCTGAAG CAGCACACCA GCTACTCGTC GCTGGCCTCC 
GAGCAGAAGG TGCACCAGCA ACAGCGCCTC GATCAACTGC ACGCCACGAA GTTGGAGTTT 
GCCAGAGAGC TAGGTGTAGA TTATGTTCCA GGTCGAGTGT GGACCAAGGA AACCTTGGGA 
AAGGATCCAA TAACAACGGC GTTTGATAAT TTGAAGCACA TTAATTTTCT GAGAAAGGGA 
TTGGCCGGCT TGAGAAGGTT TCTTCCACCA AGGACCATGA CTTTTGCTTA CTCCACTCAG 
TTGGCTCCAG GAGTTCCTCA CGAGTTTCTG TGCGATGGCA GACTGCTTAG ACTCACCGAT 
GCCATGCATC CGGATAATCG AGTTCTTTTC CAGGAGGTTT GGAAATGCGG CCAGCCAGTT 
ATGATTTCGG AGGTGGCTCG CTCTTTGAAC TTAGACTTAT GGCATCCACA AGCCTTTTGC 
CGGGACTTTG GCGACAAGCC CAATGATCTT ATTAATTGCC TGAATGGCAA TCTGGTGCCC 
AATCAGCCAA TGCGACACTT TTGGGAGGGT TTTCAGTGCA TGACCAAGCG TCTGCCAGAT 
GCATATGGCA AGCCAATGCT CTTAAAGCTT AAGGATTGGC CGCCGGGTGA TGATTTTGCC 
GAAATACTGC CCACCAGGTT TGCTGATTTG ATGAAGGGAC TGCCCATGCC GGAGTACACA 
CTTCGCACGG GAAACCTTAA CATAGCTAGC TGCTTACCCA AGATGTTTGT GCCACCAGAC 
TTAGGACCCA AAATGTACAA CGCATATGGC TCAGCATTGC ACCCCGACAA GGGTACAACC 
AATCTTCACT TGGACATTTC GGATGCAGTG AACATAATGG TTTATGTGGG AATACCCCAG 
GATGGGGACA CGAGACCTCA GATGGCGGCC ACGCAGAAAG CCATAGAAAT TGGCGGATGC 
GATTACATCA CTAGAGCTCG TTGCCAATCG CCAGATGTAT TACCCGGAGC ACTTTGGCAC 
ATCTTTCCAG CTCGCGATGC AGACAAAATA AGAGATTTAC TTAACCGCGT GACCCTGGAG 
AAGGGTTTCC GTCTGGAGCC GGATCACGAT CCAATTCACG ATCAGAATTG GTACTTGGAC 
GACAAGTTGC GAGCTCGTTT ATTCAAAGAG TATGGCGTGG AGGGGCATCC GATTGTCCAG 
TGCTTGGGCG ACGCAGTCTT TATACCAGCT GGCGCCCCAC ATCAGGTTCA AAATCTCCAC 
AACTGTATTA AGGTGGCCGA GGATTTTGTA TCGCCGGAAA ATATCACGCA CTGCTATCAT 
CTAACTCACG AGTTTAGAAG GCTTTCGCAC TCGCACACAA ATCACGAGGA CAAGCTTCAG 
ATCAAGAATA TTATCTACCA TGCCATCAAG GACTGTTGCA CCATCCTGAC CAGAGCTGTG 
GATGAGAGAC TTAATGCGGA ATTAACCAAG CTAAATGCAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287255.1
CDS	362..2926
Translation	MSQKELAALVATRTSRKRTISSTSFSISTRTDPKSIIEIHIFNDSLPIKRACCEVENPTLKAEQRVQQEEESLGQVPPLTEEEQQRHDEFRNSGSIFLQDLPCFKLQQAQPIAGSSPIPKCRECRRRNLATTEGEPSSVSDVYCRFYEFRRLQFNENGELCVVGFPNPYSEPSPEDIAIWQPDKNTPPTSGYMDIQVCRYILLHAGDQFCYIWHQEAEALSLHQNTDGTIAWKKAVKGTREICDVCDTTLFNYHWTCRKCGFGVCLDCVKDRKEGLRLRRAENAAQKGCDEYHWLLCSDPSGPQEHVLTELMLTQIIAGDALNVLGRLLHEVRTLWQVPQVCGCLLSKQAIEDAQSKEVIQDMIKESQLKQHTSYSSLASEQKVHQQQRLDQLHATKLEFARELGVDYVPGRVWTKETLGKDPITTAFDNLKHINFLRKGLAGLRRFLPPRTMTFAYSTQLAPGVPHEFLCDGRLLRLTDAMHPDNRVLFQEVWKCGQPVMISEVARSLNLDLWHPQAFCRDFGDKPNDLINCLNGNLVPNQPMRHFWEGFQCMTKRLPDAYGKPMLLKLKDWPPGDDFAEILPTRFADLMKGLPMPEYTLRTGNLNIASCLPKMFVPPDLGPKMYNAYGSALHPDKGTTNLHLDISDAVNIMVYVGIPQDGDTRPQMAATQKAIEIGGCDYITRARCQSPDVLPGALWHIFPARDADKIRDLLNRVTLEKGFRLEPDHDPIHDQNWYLDDKLRARLFKEYGVEGHPIVQCLGDAVFIPAGAPHQVQNLHNCIKVAEDFVSPENITHCYHLTHEFRRLSHSHTNHEDKLQIKNIIYHAIKDCCTILTRAVDERLNAELTKLNAD

Target ORF information:

RefSeq Version	NM_001300326.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster JmjC domain-containing histone demethylase 2 (JHDM2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300326.1

ORF Insert Sequence:

ATGTCGCAAA AAGAATTGGC GGCTCTGGTT GCCACGAGAA CGAGTCGCAA ACGCACCATA 
TCCTCGACCT CATTCTCTAT TTCCACTCGC ACTGATCCAA AATCCATCAT AGAGATACAC 
ATATTCAATG ACTCCTTGCC CATAAAAAGA GCTTGCTGCG AAGTGGAGAA TCCAACTTTA 
AAAGCGGAAC AGCGAGTGCA GCAGGAAGAA GAGAGCCTGG GCCAAGTGCC GCCGCTGACC 
GAGGAAGAGC AGCAGCGGCA CGATGAGTTC CGAAATAGTG GTTCGATATT CCTGCAGGAT 
TTGCCCTGCT TTAAGCTGCA GCAGGCACAG CCGATAGCAG GATCATCACC CATTCCCAAG 
TGTCGGGAGT GCAGAAGAAG AAATTTGGCT ACAACAGAGG GCGAACCCAG TTCAGTTAGC 
GACGTTTACT GCCGGTTCTA CGAGTTCCGA CGCCTTCAGT TCAATGAGAA TGGAGAGCTG 
TGCGTGGTTG GCTTCCCAAA TCCCTACAGC GAGCCCTCAC CAGAGGACAT CGCCATTTGG 
CAGCCGGATA AAAATACACC GCCGACCAGC GGTTACATGG ATATACAGGT TTGCAGGTAC 
ATCCTACTCC ATGCCGGCGA CCAGTTCTGC TATATTTGGC ACCAGGAAGC AGAGGCTTTA 
AGTTTGCATC AGAATACGGA CGGAACCATT GCCTGGAAGA AGGCCGTAAA AGGAACACGA 
GAGATTTGCG ACGTGTGTGA CACCACATTG TTCAACTACC ATTGGACTTG CCGCAAGTGC 
GGCTTTGGAG TGTGTCTGGA CTGCGTTAAA GATCGCAAGG AGGGATTGCG ACTGCGGCGA 
GCGGAGAACG CTGCCCAAAA GGGATGCGAT GAGTACCACT GGCTGCTCTG CAGCGACCCC 
AGTGGCCCAC AGGAGCACGT CCTTACCGAG CTGATGCTAA CACAGATCAT AGCCGGAGAT 
GCCCTCAACG TCTTGGGCAG GCTGCTGCAC GAGGTTCGTA CTTTGTGGCA GGTGCCACAG 
GTCTGCGGCT GCCTTCTGAG CAAGCAAGCG ATTGAAGATG CACAATCAAA GGAAGTGATC 
CAGGATATGA TTAAGGAGTC TCAGCTGAAG CAGCACACCA GCTACTCGTC GCTGGCCTCC 
GAGCAGAAGG TGCACCAGCA ACAGCGCCTC GATCAACTGC ACGCCACGAA GTTGGAGTTT 
GCCAGAGAGC TAGGTGTAGA TTATGTTCCA GGTCGAGTGT GGACCAAGGA AACCTTGGGA 
AAGGATCCAA TAACAACGGC GTTTGATAAT TTGAAGCACA TTAATTTTCT GAGAAAGGGA 
TTGGCCGGCT TGAGAAGGTT TCTTCCACCA AGGACCATGA CTTTTGCTTA CTCCACTCAG 
TTGGCTCCAG GAGTTCCTCA CGAGTTTCTG TGCGATGGCA GACTGCTTAG ACTCACCGAT 
GCCATGCATC CGGATAATCG AGTTCTTTTC CAGGAGGTTT GGAAATGCGG CCAGCCAGTT 
ATGATTTCGG AGGTGGCTCG CTCTTTGAAC TTAGACTTAT GGCATCCACA AGCCTTTTGC 
CGGGACTTTG GCGACAAGCC CAATGATCTT ATTAATTGCC TGAATGGCAA TCTGGTGCCC 
AATCAGCCAA TGCGACACTT TTGGGAGGGT TTTCAGTGCA TGACCAAGCG TCTGCCAGAT 
GCATATGGCA AGCCAATGCT CTTAAAGCTT AAGGATTGGC CGCCGGGTGA TGATTTTGCC 
GAAATACTGC CCACCAGGTT TGCTGATTTG ATGAAGGGAC TGCCCATGCC GGAGTACACA 
CTTCGCACGG GAAACCTTAA CATAGCTAGC TGCTTACCCA AGATGTTTGT GCCACCAGAC 
TTAGGACCCA AAATGTACAA CGCATATGGC TCAGCATTGC ACCCCGACAA GGGTACAACC 
AATCTTCACT TGGACATTTC GGATGCAGTG AACATAATGG TTTATGTGGG AATACCCCAG 
GATGGGGACA CGAGACCTCA GATGGCGGCC ACGCAGAAAG CCATAGAAAT TGGCGGATGC 
GATTACATCA CTAGAGCTCG TTGCCAATCG CCAGATGTAT TACCCGGAGC ACTTTGGCAC 
ATCTTTCCAG CTCGCGATGC AGACAAAATA AGAGATTTAC TTAACCGCGT GACCCTGGAG 
AAGGGTTTCC GTCTGGAGCC GGATCACGAT CCAATTCACG ATCAGAATTG GTACTTGGAC 
GACAAGTTGC GAGCTCGTTT ATTCAAAGAG TATGGCGTGG AGGGGCATCC GATTGTCCAG 
TGCTTGGGCG ACGCAGTCTT TATACCAGCT GGCGCCCCAC ATCAGGTTCA AAATCTCCAC 
AACTGTATTA AGGTGGCCGA GGATTTTGTA TCGCCGGAAA ATATCACGCA CTGCTATCAT 
CTAACTCACG AGTTTAGAAG GCTTTCGCAC TCGCACACAA ATCACGAGGA CAAGCTTCAG 
ATCAAGAATA TTATCTACCA TGCCATCAAG GACTGTTGCA CCATCCTGAC CAGAGCTGTG 
GATGAGAGAC TTAATGCGGA ATTAACCAAG CTAAATGCAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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JHDM2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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