Nlg4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlg4
Entrez Gene ID	42402
Full Name	Neuroligin 4
Synonyms	BcDNA:AT13703,CG15682,CG15683,CG31209,CG31499,CG34139,CG5030,CG5035,Dmel\CG34139,dnlg4
General protein information	Preferred Names Neuroligin 4 Names CG34139 gene product from transcript CG34139-RE CG34139-PB CG34139-PC CG34139-PD CG34139-PE Nlg4-PB Nlg4-PC Nlg4-PD Nlg4-PE neuroligin 4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92D5-92D8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170190.2	NP_001163661.1	neuroligin 4, isoform B
NM_001043265.2	NP_001036730.2	neuroligin 4, isoform C
NM_001170191.2	NP_001163662.1	neuroligin 4, isoform D
NM_001300491.1	NP_001287420.1	neuroligin 4, isoform E

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nlgn3	NP_599163.2
Drosophila melanogaster (fruit fly)	CG34139	NP_001036730.2
Canis lupus familiaris (dog)	NLGN3	XP_005641518.1
Mus musculus (house mouse)	Nlgn3	NP_766520.2
Xenopus tropicalis (tropical clawed frog)	nlgn3	NP_001165298.1
Homo sapiens (human)	NLGN3	NP_851820.1
Pan troglodytes (chimpanzee)	NLGN3	XP_529033.2
Gallus gallus (chicken)	NLGN3	NP_001166992.1
Danio rerio (zebrafish)	nlgn3a	NP_001159804.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003115	XP_312799.5
Bos taurus (cattle)	NLGN3	NP_001068972.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data (including data from studies using transgenic/knockout flies) suggest that nlg4 is different from nlg1-3 in that nlg4 presynaptically regulates neuromuscular junction synapse development; nlg4 appears to presynaptically regulate neuromuscular synaptic growth via BMP type I receptor signaling pathway by modulating tkv. (nlg = neuroligin; tkv = thickveins protein)
Title: Neuroligin 4 regulates synaptic growth via the bone morphogenetic protein (BMP) signaling pathway at the

DNlg4 protein, which is encoded by GH07829 cDNA (GenBank Accession Number BT050584)is essential for sleep regulation and GABA transmission.
Title: Drosophila neuroligin 4 regulates sleep through modulating GABA transmission.

The following Nlg4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlg4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05458	NM_001170191.2 Latest version!	Drosophila melanogaster neuroligin 4, transcript variant D (Nlg4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa05459	NM_001300491.1 Latest version!	Drosophila melanogaster neuroligin 4, transcript variant E (Nlg4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa05459	NM_001170190.2 Latest version!	Drosophila melanogaster neuroligin 4, transcript variant B (Nlg4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa05459	NM_001043265.2 Latest version!	Drosophila melanogaster neuroligin 4, transcript variant C (Nlg4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlg4 ( NM_001170191.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163662.1 Drosophila melanogaster neuroligin 4, transcript variant D (Nlg4), mRNA.

Price & Availability↑

CloneID	OFa05458
Clone ID Related Accession (Same CDS sequence)	NM_001170191.2
Accession Version	NM_001170191.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuroligin 4, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170191.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC 
CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC 
TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC 
AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG 
GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG 
CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA 
AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT 
GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA 
AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC 
TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA 
GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC 
CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC 
TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT 
CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC 
ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG 
CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG 
GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG 
CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC 
TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA 
GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA 
ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT 
TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT 
ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC 
GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG 
GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC 
CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA 
AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC 
GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC 
GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA 
AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT 
GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG 
GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT 
TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT 
TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC 
AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG 
TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC 
CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG 
GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC 
TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG 
GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT 
TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG 
ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG 
AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG 
CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG 
AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA 
GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG 
CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG 
GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG 
CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA 
ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC 
GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC 
AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT 
TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT 
CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT 
GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT 
GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGCAG AGCTGCAGCA ATATCAGCAG 
CAACAGCAGT CGAGCAAACA TCAGTCGAAT GGAGCTGTCC TCACTGGCAT GCAGTCGCAT 
CACATACGCG GACCACGCCC ACCGCTCCGC ACCGCCTCCT CGACGACAAC GAACAGCAGC 
AGCAATAACT CGGCAATGGG CGTGGCCGTG GGCGTAGGCG GGGGCGTTGG TAACATGCTC 
CTGGACCAAA CGCCATCCGG CGGCAGCAGC AGCAGCGGCG TAAGCAGCGC ACCCAGCTCC 
TCAAAGGGTC ACCACACCCA CTCGCACTCC CATGCCGCCC ACCTGGTGCA CTCGCACGGC 
GACGGCCTCG TCCTGACCTC CGCCTCGCCC GTGGGCGGCC AGCAACAGCA GCACCCGCAG 
CAGCATCAGC ATCCGCAGCA GCAGCAGCAA GTGCCGCAGG CGGCGATGGA CGAGATGCGC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163662.1
CDS	863..4708
Translation	MGESQLLLLLRLLLLPTVLISWMHCAGASTADIYKGARLGHRIVQTRYGRLHGLILPLDSFRFLRSVEVFLGVPYATPPTKQNRFSPTRAPAPWDGIRISDKYSPVCPQRLPNIQNETAALEKMPKGRLEYLKRLLPFLENQSEDCLYLNVFSPVNAGANEKKLPVIVFIHGESFEWSSGNPYDGSVLASYGEVVVVTLNYRLGILGFLNANPNPHAHARVANYGLMDQMAALHWIQQNIQKFGGDPNSVTLAGHGTGAACINYLMTSPTMVRGLFHRAILMSGSAYSSWALVEDPVLFAIKLAKEVNCTIPDDINRHHEQIVDCLRDVPLEDLYLADIQAPNFLTSFGPSVDGVVIRPGHSNLDIDDLMARNSRRSSADSGFQSSAGGGGGQGGGAGGGGGGGSGSSFGGGYFGGSGAGTMNMGGHYDVLFGVVTGESIWRFSAHDIQNGFEGERRDKIIRTYVRNAYNYHLNEIFYTIVNEYTDWDRTSQHPINTRDTAVAALSDAQFVAPIVRAGDILAANSPPPVSSSSTAGSPGANAAASTSAGSTQPSGRCYFYVFDYQTKDGDYPQRMGTVHGEDLPYIFGAPLVDGFSHFPQNYTKSETALSEAVMIFWTNFARTGNPNEHHRQDSSLPVSKERNRFRSITWENYDPLHQKYLEIGMKPRIKNHFRAHQLSIWLRLIPELHRAGMEDVIARHNLFRNHDDMDLYEGPVKPDPFGISTAAGASGSSSSSSSSSRLLLVDEQLMMKKGRGLNGSAYLNGILGVTTVEPNNMYTTCIPIGGNYSSSGGVFAPTTLANASSDTLASGFEAAGYAAYSTALSVTIAIGCSLLILNVLIFAGVYYQRDKTRLEVKTLQKQYQQRSLHQQVPYPPEPIKHAHYHMGHSQSSANVIVDVESHQDQAGQAAMLLQAAAAAAAAAANDVKPPPPHICSNTGMQQQQQVGGGSGGALNNGGIEGSKVTTDNLGNVTYSTSSKQQQQHQQQQQQQQREHMQIKGMTGTQTFGRSGSGSGGGAGGGAGSASGGASVVVSGSSVSYNPGMMTLPKSGGLHHAATLNYARNTTALNMSGGGTALVDSRGNVLLTSTAVGPGMAGTGAGGGSSGGGGGGDCMTLPRNLGLAAAGRHNPTTAELQQYQQQQQSSKHQSNGAVLTGMQSHHIRGPRPPLRTASSTTTNSSSNNSAMGVAVGVGGGVGNMLLDQTPSGGSSSSGVSSAPSSSKGHHTHSHSHAAHLVHSHGDGLVLTSASPVGGQQQQHPQQHQHPQQQQQVPQAAMDEMRV

Target ORF information:

RefSeq Version	NM_001170191.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuroligin 4, transcript variant D (Nlg4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170191.2

ORF Insert Sequence:

ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC 
CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC 
TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC 
AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG 
GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG 
CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA 
AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT 
GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA 
AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC 
TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA 
GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC 
CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC 
TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT 
CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC 
ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG 
CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG 
GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG 
CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC 
TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA 
GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA 
ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT 
TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT 
ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC 
GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG 
GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC 
CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA 
AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC 
GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC 
GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA 
AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT 
GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG 
GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT 
TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT 
TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC 
AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG 
TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC 
CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG 
GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC 
TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG 
GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT 
TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG 
ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG 
AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG 
CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG 
AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA 
GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG 
CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG 
GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG 
CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA 
ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC 
GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC 
AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT 
TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT 
CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT 
GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT 
GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGCAG AGCTGCAGCA ATATCAGCAG 
CAACAGCAGT CGAGCAAACA TCAGTCGAAT GGAGCTGTCC TCACTGGCAT GCAGTCGCAT 
CACATACGCG GACCACGCCC ACCGCTCCGC ACCGCCTCCT CGACGACAAC GAACAGCAGC 
AGCAATAACT CGGCAATGGG CGTGGCCGTG GGCGTAGGCG GGGGCGTTGG TAACATGCTC 
CTGGACCAAA CGCCATCCGG CGGCAGCAGC AGCAGCGGCG TAAGCAGCGC ACCCAGCTCC 
TCAAAGGGTC ACCACACCCA CTCGCACTCC CATGCCGCCC ACCTGGTGCA CTCGCACGGC 
GACGGCCTCG TCCTGACCTC CGCCTCGCCC GTGGGCGGCC AGCAACAGCA GCACCCGCAG 
CAGCATCAGC ATCCGCAGCA GCAGCAGCAA GTGCCGCAGG CGGCGATGGA CGAGATGCGC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlg4 ( NM_001170190.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163661.1 Drosophila melanogaster neuroligin 4, transcript variant B (Nlg4), mRNA.

Price & Availability↑

CloneID	OFa05459
Clone ID Related Accession (Same CDS sequence)	NM_001170190.2 , NM_001043265.2 , NM_001300491.1
Accession Version	NM_001170190.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuroligin 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170190.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC 
CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC 
TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC 
AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG 
GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG 
CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA 
AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT 
GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA 
AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC 
TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA 
GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC 
CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC 
TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT 
CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC 
ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG 
CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG 
GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG 
CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC 
TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA 
GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA 
ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT 
TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT 
ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC 
GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG 
GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC 
CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA 
AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC 
GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC 
GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA 
AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT 
GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG 
GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT 
TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT 
TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC 
AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG 
TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC 
CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG 
GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC 
TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG 
GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT 
TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG 
ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG 
AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG 
CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG 
AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA 
GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG 
CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG 
GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG 
CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA 
ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC 
GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC 
AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT 
TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT 
CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT 
GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT 
GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA 
CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC 
ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC 
AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG 
GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA 
AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC 
GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG 
CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163661.1
CDS	863..4705
Translation	MGESQLLLLLRLLLLPTVLISWMHCAGASTADIYKGARLGHRIVQTRYGRLHGLILPLDSFRFLRSVEVFLGVPYATPPTKQNRFSPTRAPAPWDGIRISDKYSPVCPQRLPNIQNETAALEKMPKGRLEYLKRLLPFLENQSEDCLYLNVFSPVNAGANEKKLPVIVFIHGESFEWSSGNPYDGSVLASYGEVVVVTLNYRLGILGFLNANPNPHAHARVANYGLMDQMAALHWIQQNIQKFGGDPNSVTLAGHGTGAACINYLMTSPTMVRGLFHRAILMSGSAYSSWALVEDPVLFAIKLAKEVNCTIPDDINRHHEQIVDCLRDVPLEDLYLADIQAPNFLTSFGPSVDGVVIRPGHSNLDIDDLMARNSRRSSADSGFQSSAGGGGGQGGGAGGGGGGGSGSSFGGGYFGGSGAGTMNMGGHYDVLFGVVTGESIWRFSAHDIQNGFEGERRDKIIRTYVRNAYNYHLNEIFYTIVNEYTDWDRTSQHPINTRDTAVAALSDAQFVAPIVRAGDILAANSPPPVSSSSTAGSPGANAAASTSAGSTQPSGRCYFYVFDYQTKDGDYPQRMGTVHGEDLPYIFGAPLVDGFSHFPQNYTKSETALSEAVMIFWTNFARTGNPNEHHRQDSSLPVSKERNRFRSITWENYDPLHQKYLEIGMKPRIKNHFRAHQLSIWLRLIPELHRAGMEDVIARHNLFRNHDDMDLYEGPVKPDPFGISTAAGASGSSSSSSSSSRLLLVDEQLMMKKGRGLNGSAYLNGILGVTTVEPNNMYTTCIPIGGNYSSSGGVFAPTTLANASSDTLASGFEAAGYAAYSTALSVTIAIGCSLLILNVLIFAGVYYQRDKTRLEVKTLQKQYQQRSLHQQVPYPPEPIKHAHYHMGHSQSSANVIVDVESHQDQAGQAAMLLQAAAAAAAAAANDVKPPPPHICSNTGMQQQQQVGGGSGGALNNGGIEGSKVTTDNLGNVTYSTSSKQQQQHQQQQQQQQREHMQIKGMTGTQTFGRSGSGSGGGAGGGAGSASGGASVVVSGSSVSYNPGMMTLPKSGGLHHAATLNYARNTTALNMSGGGTALVDSRGNVLLTSTAVGPGMAGTGAGGGSSGGGGGGDCMTLPRNLGLAAAGRHNPTTELQQYQQQQQSSKHQSNGAVLTGMQSHHIRGPRPPLRTASSTTTNSSSNNSAMGVAVGVGGGVGNMLLDQTPSGGSSSSGVSSAPSSSKGHHTHSHSHAAHLVHSHGDGLVLTSASPVGGQQQQHPQQHQHPQQQQQVPQAAMDEMRV

Target ORF information:

RefSeq Version	NM_001170190.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuroligin 4, transcript variant B (Nlg4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170190.2

ORF Insert Sequence:

ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC 
CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC 
TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC 
AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG 
GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG 
CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA 
AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT 
GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA 
AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC 
TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA 
GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC 
CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC 
TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT 
CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC 
ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG 
CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG 
GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG 
CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC 
TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA 
GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA 
ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT 
TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT 
ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC 
GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG 
GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC 
CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA 
AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC 
GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC 
GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA 
AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT 
GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG 
GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT 
TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT 
TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC 
AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG 
TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC 
CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG 
GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC 
TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG 
GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT 
TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG 
ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG 
AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG 
CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG 
AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA 
GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG 
CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG 
GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG 
CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA 
ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC 
GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC 
AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT 
TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT 
CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT 
GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT 
GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA 
CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC 
ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC 
AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG 
GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA 
AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC 
GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG 
CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlg4 ( NM_001043265.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036730.2 Drosophila melanogaster neuroligin 4, transcript variant C (Nlg4), mRNA.

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CloneID	OFa05459
Clone ID Related Accession (Same CDS sequence)	NM_001170190.2 , NM_001043265.2 , NM_001300491.1
Accession Version	NM_001043265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuroligin 4, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_001043265.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC 
CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC 
TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC 
AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG 
GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG 
CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA 
AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT 
GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA 
AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC 
TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA 
GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC 
CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC 
TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT 
CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC 
ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG 
CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG 
GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG 
CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC 
TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA 
GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA 
ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT 
TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT 
ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC 
GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG 
GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC 
CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA 
AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC 
GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC 
GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA 
AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT 
GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG 
GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT 
TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT 
TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC 
AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG 
TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC 
CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG 
GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC 
TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG 
GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT 
TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG 
ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG 
AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG 
CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG 
AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA 
GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG 
CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG 
GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG 
CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA 
ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC 
GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC 
AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT 
TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT 
CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT 
GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT 
GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA 
CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC 
ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC 
AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG 
GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA 
AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC 
GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG 
CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036730.2
CDS	882..4724
Translation	MGESQLLLLLRLLLLPTVLISWMHCAGASTADIYKGARLGHRIVQTRYGRLHGLILPLDSFRFLRSVEVFLGVPYATPPTKQNRFSPTRAPAPWDGIRISDKYSPVCPQRLPNIQNETAALEKMPKGRLEYLKRLLPFLENQSEDCLYLNVFSPVNAGANEKKLPVIVFIHGESFEWSSGNPYDGSVLASYGEVVVVTLNYRLGILGFLNANPNPHAHARVANYGLMDQMAALHWIQQNIQKFGGDPNSVTLAGHGTGAACINYLMTSPTMVRGLFHRAILMSGSAYSSWALVEDPVLFAIKLAKEVNCTIPDDINRHHEQIVDCLRDVPLEDLYLADIQAPNFLTSFGPSVDGVVIRPGHSNLDIDDLMARNSRRSSADSGFQSSAGGGGGQGGGAGGGGGGGSGSSFGGGYFGGSGAGTMNMGGHYDVLFGVVTGESIWRFSAHDIQNGFEGERRDKIIRTYVRNAYNYHLNEIFYTIVNEYTDWDRTSQHPINTRDTAVAALSDAQFVAPIVRAGDILAANSPPPVSSSSTAGSPGANAAASTSAGSTQPSGRCYFYVFDYQTKDGDYPQRMGTVHGEDLPYIFGAPLVDGFSHFPQNYTKSETALSEAVMIFWTNFARTGNPNEHHRQDSSLPVSKERNRFRSITWENYDPLHQKYLEIGMKPRIKNHFRAHQLSIWLRLIPELHRAGMEDVIARHNLFRNHDDMDLYEGPVKPDPFGISTAAGASGSSSSSSSSSRLLLVDEQLMMKKGRGLNGSAYLNGILGVTTVEPNNMYTTCIPIGGNYSSSGGVFAPTTLANASSDTLASGFEAAGYAAYSTALSVTIAIGCSLLILNVLIFAGVYYQRDKTRLEVKTLQKQYQQRSLHQQVPYPPEPIKHAHYHMGHSQSSANVIVDVESHQDQAGQAAMLLQAAAAAAAAAANDVKPPPPHICSNTGMQQQQQVGGGSGGALNNGGIEGSKVTTDNLGNVTYSTSSKQQQQHQQQQQQQQREHMQIKGMTGTQTFGRSGSGSGGGAGGGAGSASGGASVVVSGSSVSYNPGMMTLPKSGGLHHAATLNYARNTTALNMSGGGTALVDSRGNVLLTSTAVGPGMAGTGAGGGSSGGGGGGDCMTLPRNLGLAAAGRHNPTTELQQYQQQQQSSKHQSNGAVLTGMQSHHIRGPRPPLRTASSTTTNSSSNNSAMGVAVGVGGGVGNMLLDQTPSGGSSSSGVSSAPSSSKGHHTHSHSHAAHLVHSHGDGLVLTSASPVGGQQQQHPQQHQHPQQQQQVPQAAMDEMRV

Target ORF information:

RefSeq Version	NM_001043265.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuroligin 4, transcript variant C (Nlg4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043265.2

ORF Insert Sequence:

ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC 
CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC 
TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC 
AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG 
GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG 
CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA 
AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT 
GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA 
AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC 
TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA 
GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC 
CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC 
TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT 
CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC 
ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG 
CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG 
GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG 
CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC 
TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA 
GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA 
ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT 
TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT 
ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC 
GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG 
GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC 
CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA 
AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC 
GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC 
GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA 
AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT 
GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG 
GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT 
TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT 
TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC 
AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG 
TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC 
CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG 
GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC 
TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG 
GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT 
TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG 
ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG 
AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG 
CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG 
AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA 
GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG 
CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG 
GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG 
CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA 
ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC 
GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC 
AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT 
TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT 
CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT 
GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT 
GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA 
CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC 
ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC 
AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG 
GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA 
AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC 
GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG 
CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlg4 ( NM_001300491.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287420.1 Drosophila melanogaster neuroligin 4, transcript variant E (Nlg4), mRNA.

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CloneID	OFa05459
Clone ID Related Accession (Same CDS sequence)	NM_001170190.2 , NM_001043265.2 , NM_001300491.1
Accession Version	NM_001300491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuroligin 4, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC 
CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC 
TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC 
AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG 
GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG 
CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA 
AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT 
GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA 
AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC 
TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA 
GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC 
CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC 
TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT 
CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC 
ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG 
CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG 
GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG 
CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC 
TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA 
GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA 
ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT 
TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT 
ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC 
GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG 
GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC 
CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA 
AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC 
GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC 
GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA 
AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT 
GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG 
GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT 
TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT 
TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC 
AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG 
TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC 
CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG 
GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC 
TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG 
GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT 
TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG 
ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG 
AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG 
CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG 
AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA 
GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG 
CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG 
GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG 
CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA 
ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC 
GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC 
AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT 
TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT 
CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT 
GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT 
GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA 
CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC 
ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC 
AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG 
GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA 
AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC 
GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG 
CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287420.1
CDS	863..4705
Translation	MGESQLLLLLRLLLLPTVLISWMHCAGASTADIYKGARLGHRIVQTRYGRLHGLILPLDSFRFLRSVEVFLGVPYATPPTKQNRFSPTRAPAPWDGIRISDKYSPVCPQRLPNIQNETAALEKMPKGRLEYLKRLLPFLENQSEDCLYLNVFSPVNAGANEKKLPVIVFIHGESFEWSSGNPYDGSVLASYGEVVVVTLNYRLGILGFLNANPNPHAHARVANYGLMDQMAALHWIQQNIQKFGGDPNSVTLAGHGTGAACINYLMTSPTMVRGLFHRAILMSGSAYSSWALVEDPVLFAIKLAKEVNCTIPDDINRHHEQIVDCLRDVPLEDLYLADIQAPNFLTSFGPSVDGVVIRPGHSNLDIDDLMARNSRRSSADSGFQSSAGGGGGQGGGAGGGGGGGSGSSFGGGYFGGSGAGTMNMGGHYDVLFGVVTGESIWRFSAHDIQNGFEGERRDKIIRTYVRNAYNYHLNEIFYTIVNEYTDWDRTSQHPINTRDTAVAALSDAQFVAPIVRAGDILAANSPPPVSSSSTAGSPGANAAASTSAGSTQPSGRCYFYVFDYQTKDGDYPQRMGTVHGEDLPYIFGAPLVDGFSHFPQNYTKSETALSEAVMIFWTNFARTGNPNEHHRQDSSLPVSKERNRFRSITWENYDPLHQKYLEIGMKPRIKNHFRAHQLSIWLRLIPELHRAGMEDVIARHNLFRNHDDMDLYEGPVKPDPFGISTAAGASGSSSSSSSSSRLLLVDEQLMMKKGRGLNGSAYLNGILGVTTVEPNNMYTTCIPIGGNYSSSGGVFAPTTLANASSDTLASGFEAAGYAAYSTALSVTIAIGCSLLILNVLIFAGVYYQRDKTRLEVKTLQKQYQQRSLHQQVPYPPEPIKHAHYHMGHSQSSANVIVDVESHQDQAGQAAMLLQAAAAAAAAAANDVKPPPPHICSNTGMQQQQQVGGGSGGALNNGGIEGSKVTTDNLGNVTYSTSSKQQQQHQQQQQQQQREHMQIKGMTGTQTFGRSGSGSGGGAGGGAGSASGGASVVVSGSSVSYNPGMMTLPKSGGLHHAATLNYARNTTALNMSGGGTALVDSRGNVLLTSTAVGPGMAGTGAGGGSSGGGGGGDCMTLPRNLGLAAAGRHNPTTELQQYQQQQQSSKHQSNGAVLTGMQSHHIRGPRPPLRTASSTTTNSSSNNSAMGVAVGVGGGVGNMLLDQTPSGGSSSSGVSSAPSSSKGHHTHSHSHAAHLVHSHGDGLVLTSASPVGGQQQQHPQQHQHPQQQQQVPQAAMDEMRV

Target ORF information:

RefSeq Version	NM_001300491.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuroligin 4, transcript variant E (Nlg4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300491.1

ORF Insert Sequence:

ATGGGGGAAA GTCAGCTGCT GCTGTTGCTG CGGCTCCTGC TGCTTCCTAC AGTCCTCATT 
TCCTGGATGC ACTGTGCCGG GGCCTCCACG GCGGACATCT ACAAGGGCGC TCGCCTGGGC 
CACCGTATCG TTCAGACGCG CTATGGCCGA CTTCATGGAC TCATCCTGCC CCTGGATAGC 
TTTCGCTTCC TGCGATCCGT AGAGGTCTTC CTGGGTGTGC CATATGCAAC GCCTCCCACC 
AAACAAAATC GGTTTAGTCC CACGAGAGCT CCAGCGCCTT GGGACGGCAT CCGGATATCG 
GATAAATACA GTCCCGTGTG TCCGCAGCGT TTGCCCAATA TCCAAAATGA GACGGCGGCG 
CTGGAGAAGA TGCCCAAGGG TCGCCTGGAG TACTTAAAGA GATTGCTGCC ATTTCTTGAA 
AACCAGTCCG AGGACTGCCT GTACCTAAAT GTGTTCTCGC CGGTCAATGC CGGGGCCAAT 
GAGAAGAAGT TGCCGGTGAT TGTGTTCATA CACGGCGAGT CCTTCGAGTG GAGCAGCGGA 
AATCCTTACG ACGGCAGTGT CCTGGCGAGC TACGGGGAAG TTGTGGTGGT AACACTCAAC 
TACCGACTCG GAATATTAGG TTTCCTCAAC GCCAATCCCA ATCCACATGC CCATGCCAGA 
GTGGCCAACT ACGGGCTGAT GGACCAGATG GCTGCCCTGC ATTGGATTCA GCAGAATATC 
CAAAAGTTTG GCGGAGATCC CAATTCGGTG ACGTTGGCGG GTCATGGAAC GGGTGCAGCC 
TGTATTAATT ACCTAATGAC CTCGCCTACA ATGGTCCGTG GTCTCTTCCA CCGGGCTATT 
CTCATGTCCG GATCAGCGTA CTCATCGTGG GCTTTGGTCG AGGATCCAGT ACTATTTGCC 
ATCAAGCTGG CCAAGGAGGT TAACTGCACC ATTCCCGATG ATATCAATCG CCACCACGAG 
CAGATTGTCG ATTGTCTGAG GGATGTTCCC CTGGAAGACC TTTACCTAGC CGACATTCAG 
GCTCCAAATT TTCTCACATC CTTCGGGCCT TCGGTTGATG GCGTTGTCAT ACGACCCGGG 
CATTCCAATC TCGATATCGA TGATTTAATG GCGCGCAACT CGAGGCGCTC GTCAGCGGAC 
TCCGGTTTCC AGTCGTCCGC CGGAGGTGGT GGCGGGCAAG GAGGCGGGGC CGGTGGCGGA 
GGAGGCGGTG GCTCCGGATC GTCGTTCGGC GGTGGATATT TCGGCGGCAG CGGGGCCGGA 
ACGATGAATA TGGGCGGTCA CTATGACGTC CTGTTTGGCG TCGTTACGGG CGAATCGATT 
TGGCGCTTCA GTGCCCACGA TATACAAAAT GGTTTCGAGG GCGAGAGACG CGATAAAATT 
ATCCGCACCT ACGTCCGCAA CGCCTACAAT TATCACCTCA ACGAGATTTT CTATACGATC 
GTCAACGAGT ACACGGATTG GGATCGCACC TCGCAGCATC CGATCAATAC CCGCGATACG 
GCTGTAGCCG CCCTATCCGA TGCCCAGTTT GTGGCACCGA TTGTGCGGGC TGGTGATATC 
CTGGCGGCCA ATTCCCCGCC GCCGGTCAGC TCATCCTCGA CCGCTGGGTC GCCGGGTGCA 
AATGCGGCTG CTTCGACCTC AGCGGGCTCC ACTCAGCCCT CCGGTCGGTG CTACTTTTAC 
GTGTTCGATT ACCAGACAAA GGACGGCGAC TATCCACAGA GAATGGGCAC TGTGCACGGC 
GAGGACTTGC CCTACATTTT CGGCGCCCCA CTGGTCGACG GCTTTAGTCA CTTTCCGCAA 
AACTACACCA AATCGGAGAC GGCTCTTTCC GAGGCGGTGA TGATATTCTG GACCAACTTT 
GCTCGCACCG GCAATCCCAA TGAACACCAT CGCCAGGACT CGTCGCTGCC GGTTTCAAAG 
GAGCGCAACC GCTTTCGCAG CATCACCTGG GAAAATTATG ATCCGCTGCA CCAGAAGTAT 
TTGGAAATAG GAATGAAGCC GCGCATTAAG AATCACTTTC GGGCCCATCA ACTCTCGATT 
TGGCTGCGCC TGATACCGGA GCTTCATCGC GCCGGGATGG AGGATGTGAT AGCCCGGCAC 
AATCTGTTCC GCAATCACGA CGATATGGAC CTGTACGAGG GACCCGTTAA ACCGGATCCG 
TTTGGCATAT CGACAGCCGC CGGAGCAAGT GGCTCCTCAT CCTCGTCGTC GTCCTCATCC 
CGCCTTCTGC TCGTCGATGA GCAATTGATG ATGAAGAAGG GACGCGGCCT GAACGGCTCG 
GCCTATTTGA ACGGCATATT GGGCGTGACC ACCGTGGAGC CGAACAACAT GTACACGACC 
TGCATACCCA TTGGGGGAAA CTACTCGAGC AGCGGTGGTG TCTTCGCACC CACCACTCTG 
GCCAACGCCT CCTCGGACAC TCTGGCCTCC GGATTCGAGG CAGCCGGCTA TGCGGCCTAT 
TCGACGGCCC TGAGTGTTAC GATTGCCATT GGCTGCAGCC TGCTCATACT GAACGTGTTG 
ATTTTCGCCG GAGTCTACTA TCAGCGCGAC AAGACGCGGC TGGAGGTGAA GACACTGCAG 
AAGCAGTACC AGCAGCGTAG CCTGCACCAG CAGGTCCCCT ACCCACCGGA GCCCATCAAG 
CACGCCCACT ACCACATGGG TCACTCGCAG AGCTCGGCCA ATGTCATCGT CGATGTGGAG 
AGCCATCAGG ACCAGGCCGG CCAGGCGGCC ATGTTGCTCC AAGCCGCAGC AGCAGCCGCA 
GCAGCAGCGG CCAACGACGT GAAGCCACCG CCACCGCACA TCTGCTCAAA CACAGGAATG 
CAACAGCAGC AGCAAGTGGG TGGTGGCAGC GGTGGTGCCC TGAATAATGG TGGCATCGAG 
GGGTCCAAGG TGACCACAGA CAACCTGGGC AACGTCACCT ACAGCACTAG CTCCAAGCAG 
CAGCAGCAAC ACCAGCAACA ACAGCAGCAG CAGCAGCGTG AGCACATGCA AATCAAGGGA 
ATGACCGGCA CGCAAACATT CGGACGAAGT GGCTCCGGAT CGGGAGGCGG GGCTGGGGGC 
GGAGCCGGTT CCGCTTCCGG TGGCGCCTCC GTGGTGGTGT CCGGCAGCAG CGTTAGCTAC 
AATCCCGGCA TGATGACGCT GCCCAAGTCG GGCGGTCTCC ATCATGCGGC CACTTTGAAT 
TATGCGCGCA ACACGACCGC CTTGAATATG TCGGGCGGCG GCACCGCCCT AGTGGATAGT 
CGCGGCAATG TCCTGCTCAC CAGCACCGCA GTGGGTCCTG GCATGGCCGG TACTGGTGCT 
GGAGGAGGAT CCTCTGGTGG CGGCGGAGGC GGAGATTGCA TGACCCTGCC ACGCAATCTT 
GGTCTGGCGG CCGCAGGACG ACACAATCCC ACTACAGAGC TGCAGCAATA TCAGCAGCAA 
CAGCAGTCGA GCAAACATCA GTCGAATGGA GCTGTCCTCA CTGGCATGCA GTCGCATCAC 
ATACGCGGAC CACGCCCACC GCTCCGCACC GCCTCCTCGA CGACAACGAA CAGCAGCAGC 
AATAACTCGG CAATGGGCGT GGCCGTGGGC GTAGGCGGGG GCGTTGGTAA CATGCTCCTG 
GACCAAACGC CATCCGGCGG CAGCAGCAGC AGCGGCGTAA GCAGCGCACC CAGCTCCTCA 
AAGGGTCACC ACACCCACTC GCACTCCCAT GCCGCCCACC TGGTGCACTC GCACGGCGAC 
GGCCTCGTCC TGACCTCCGC CTCGCCCGTG GGCGGCCAGC AACAGCAGCA CCCGCAGCAG 
CATCAGCATC CGCAGCAGCA GCAGCAAGTG CCGCAGGCGG CGATGGACGA GATGCGCGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nlg4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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