Fancm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fancm
Entrez Gene ID	42543
Full Name	Fanconi anemia group M helicase
Synonyms	CG7922,Dmel\CG7922,cg7922
General protein information	Preferred Names Fanconi anemia group M helicase Names CG7922 gene product from transcript CG7922-RB CG7922-PA CG7922-PB Fancm-PA Fancm-PB fanconi anemia group M (FANCM) helicase fanconi anemia group M helicase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 93E2-93E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_142714.3	NP_650971.2	fanconi anemia group M helicase, isoform A
NM_001275877.1	NP_001262806.1	fanconi anemia group M helicase, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using transgenic flies expressing full-length or truncated Fancm, each with either a wild-type or mutated helicase domain, we found that there are helicase-independent and C-terminal-independent functions in responding to DNA damage and in preventing mitotic crossovers
Title: Biochemical Activities and Genetic Functions of the Drosophila melanogaster Fancm Helicase in DNA Repair.

spontaneous mitotic crossovers that occur when FANCM is missing are dependent on MUS312 and either MUS81 or SLX1.
Title: Drosophila FANCM helicase prevents spontaneous mitotic crossovers generated by the MUS81 and SLX1 nucleases.

The following Fancm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fancm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05603	NM_142714.3 Latest version!	Drosophila melanogaster fanconi anemia group M helicase, transcript variant A (Fancm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa05603	NM_001275877.1 Latest version!	Drosophila melanogaster fanconi anemia group M helicase, transcript variant B (Fancm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fancm ( NM_142714.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650971.2 Drosophila melanogaster fanconi anemia group M helicase, transcript variant A (Fancm), mRNA.

Price & Availability↑

CloneID	OFa05603
Clone ID Related Accession (Same CDS sequence)	NM_142714.3 , NM_001275877.1
Accession Version	NM_142714.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fanconi anemia group M helicase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_142714.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGTGA ATTGGATGGA CGATGACGAA GACCTAGTGG CGGCCTTGGC TATGCACGAA 
GAGCAGAAGA GCGAGGATGC CGATGGAACA CGTTGGGAAC ATTGCGACGA AGCCTGCGAT 
GGTTTCGATA TGGCCACAGG ACACAACTGG ATTTATCCAA ACAACCTGCC CCTACGATCC 
TATCAACAAA CCATCGTCCA ATCGGCGCTG TTCAAAAACA CCCTGGTGGT GTTGCCCACG 
GGATTGGGAA AGACCTTCAT TGCGGCGGTG GTTATGTACA ACTTTTACAG GTGGTATCCA 
AAGGGAAAGA TTGTGTTCAT GGCACCCACA AGGCCCCTGG TCTCGCAGCA GATCCACGCA 
TCCCAGAAGA TCATGCCCTT TCCATCTGAA GACACGGTGC AGCTAACAGG CCAACTTCCT 
CGACCCAAAC GAGCTGAGCT GTGGGCCTCC AAGCGGGTCT TCTTCGCTAC GCCGCAGGTG 
GTGCACTCAG ACATGCTGGA AGCTGATGGC GAATGCAGCT TTCCCTTTGG GTCCATCAAG 
TTGATAGTGG TGGATGAGGC GCACCGGGCC AAAGGTCGCT ATGCCTACAC CCAAGTGGCC 
GACTGTCTGA TGGCACGTAA CAGGTATTTC CGGATGCTTG CCCTGTCCGC TACACCAGGA 
AGAACCATGG AGGATGTGGC AGCTGTGTGC CGAAACCTAT ACATTTCCAA TCTGCAAGTG 
CGCTGGGACA CCTCGATCGA CGTGCAGCCC TACATACACA GGCGGACGAT TCGAACCATA 
GTGGTCTCCC TGAAGGAGAG AATCAAGGAG CCGAGAGAAC GATTGCTGCA GATCATCGAA 
CCATACCTAC GCCAGCTTAT GGAAGCTGAG ATTTTTAAAG GCAACAAGGG AACCGTGAGC 
AGAAATAGTT TGCTCTTTGA GCAGAAAAGC TTCGTCGAAC GATCGGCGCA AGGCCAGCGG 
CATCCGGATC ACAACATAAT AATGGGCAAC TTCGCCATGT GCATCAGCAT GTACCACTCG 
CTGGACCTAA TGGAGCGTCA CGGTCTTCGC GTCTTCGTGA ACAATTTCGA TGCAGACGAC 
GACGGGCGAG AGAAATTTGT ACTGGCCAGA GATGGTAATC TAAGGAATTT GGTGGAGCAG 
GTCCGCCAAG AACTCGGTGC CAATCCCCTA GATTACACCA CGCATGCCAT GACGAATGGA 
GAAGTGCCGC CTTTGCCATC TGATCTAGAC TTTGGACATG CCAAATATGA GAAGTTGCGA 
CAGGTGTTGG TGCAACACTT TCAGGCAAAC CCCGATTCCC GCGCCATTGT TTTCTGCGAG 
TACCGTGAAT CCGTAATGCT GATTCACCGG CTACTCCTTC AACACAGACC GGTGCTCCGT 
CCGCGCTGTT TTGTTGGCCA GGGCAGCACA GTTGGAGCAA GCTATGCTCT CACCCAGAAA 
CAGCAGCTGC AGATAATGAC CGACTTTCGA TCCGGCACTA GCAACGTTCT GGTGGCCACG 
TCGATCGGAG AGGAGGGCTT AGACGTCGGC GAGGTGGAGA TGATTGTGTG CTTCGACATT 
TGCAGCACTA ATCCCACTAG ATTTATTCAG CGGATTGGGC GCACTGGAAG GAAGAAGAAT 
GGAGAGGTGG TTATGCTAGT GACCGAAGGA CGAGAACAGC AGGTGCTTAA GGATGTTCTG 
GCCAACAAGG ATCAAATCAA CAAGAAGCTA TTGAATTCGT CCGTTGTTAA ATTGTCGCTT 
TATGAGCAAA ATCCTCGAAT GGTGCCATCA AAATTTCAAC CAAAATGCGA GGAGAAACAC 
ATGGAGCCAG TTGCTGAGGA GAAACCCAAG CCGAAGTCGG CAGCCAAGAC GAAAGAATCC 
AGGAAGCGGA AGCAACCAGT TGCACAGACT GGTAGCCTAA GGAAATACTT CAAAGAAAGT 
CCTCCAACGG AGAGTCAGCA AGGAATATTG CAGGGAATAA AACCCTACCA AATGAGCGAA 
GCTTCACAGC AATTGGTTAA GCAGCAAGTT CTACGGCGCA GCGTAACTCT GAAGAACTTC 
TTTGGGGAAT CTCAGGCCAG CTCAACGTTA ACCAGCAGTC AGGAAGATGT GCAACGCCTC 
CGAAAACTTA CTCGCCTTCT GCAGTCAAGC AAGCCATTTG TTTCGGACTC CAAGGATCTT 
ATGAGCCATC TGCAGGACGA TCACTTACCC AGGCAAATAA AGCTCTACTT ACTCAAAAGT 
AATCCCGAAT TTCTGAGGGA AACCCATTTG AAGATGCAAG TCCAGAGCGA GTTGAATATA 
GCTGATGATC GCTTAAATAG TCGGCAGCGA CGCACTAAAA ATAATTATCA ACTGCTTCTG 
GACATTTGCG ACGGAATGGA CCAGATGAAT GAGCTTCTTC AAGGGGATAA TAGTGGAGCA 
GAGATTAGCT TCAGGGATGA ACTAAATCCT ATTTATAATC AAAGTCCCAA AAAGTTCGAT 
ACCATTTGTG AACAGATTTT CGATGGTCTG AATGAGCATG GCCTAAACTC GAACAATTTT 
GAACTGAAGC AAGATCTTTT AGAGAAACTT GAGCTTCGCA ATCTGGAAAC CACAGTAAAC 
GAGCAACTGG GTGGCATTGA AGCTTCCTGG TCAGAAGAGG AGTGGGACCA ACAGGAGGAA 
GACGTGAAAT TTGAGTCTAT GTACCTAAGC CAGCAATTGA ACTTACCAGA CGCGAATGTT 
GTCCCCCATA GCTCAACGCC GCTCAGGGTC AAACCCTTGA GCAAATTAAT GTTTAAAACC 
CTGCAGGGCG ATATTGAAGA GGAGTATCAT TCCGGGATGG AAGCTAGTGA ATTGGGGGAC 
AACTTAGGTC GCCTAAATTC ACTGATGAAC GCAAGTGAAT CGAAGATTAA AGCGACTGAA 
AGAATTGCAC CTATTCCTGT AGTAGACTCT ACAATTGAGA CAAATCACTT TCCAGCCGTG 
GAAGAAAGTC AAAGATCAAC TCCTATTTCC ATTGCAGATT CTTCAGGTGA ATCAAATCAC 
TGTGCTGTTA ATAAAAATAG TGAGATATAT CCAATGAGCA TAGATGAGAC GAAAGTAGAA 
GTTAAGTCCT CTTTAAATCT GAAAATGCAC GCAGAATCCA TTGTAGAAGA TTTGGATATC 
GACTTGGATG ATTTTCTAGA GCCAATGGAC AAGGAACTGG AATTAGCCTC CCAGACAAAA 
GGGAAGTCGC AAGAGAACAT CGTAGATCAC CAGATGGAAA AATTGGAGAA AAACTGCGAG 
CAGAAAGAAA CAGCACACAA CAATGACCTT GATCTAACGG ACGAGGACTT GAAAGAATTT 
CTAGAGCCGA TGGTGGAGGA AGTGGAGTTG ATGAGCCAAA AAAAGGCGAA TGACTTCAAG 
GATTTTCTCG AGCCAATGCC AGAGGAGTTA GAGCTAATGA GCCAGCAGAA GAAAAACATA 
CTGCTTCCAA GTACAGAAAC AATTAAAAAC TCAGAAAATA AAAACTCACA TGAGGATGGC 
TCAAGAACCG TTTCGCCAGA TATCTTTGGA TCTGACTCCA TGTCACCTTT GAAGCCCCAA 
GGAAAAAGTC TGGCCGCAAA ATTGGCAGCC AAGGCAGCAG CAAAGGTTTT GCCTTGCCCA 
CCGCCGAATT CAGTAGGTCT TAATTCGCCG GAGAACCGGA AACGTACCAA TCCTTCTATC 
CAGGAAAAGT CTCCAAGTAT CTTTGATCTA TATTTAAATC GAATGCGGGG TCGTGGTCGT 
CTTGCTAAAG CTGCGGAGAA TCTTCACCGT ATAACTTCAA CGAATCCTAC AATCTCAGTA 
CCTAAAGACG AAGAGGATTC TCCTATTGCG CGGCGTCCAT CCAAACGAAA GATTGTTATA 
AGTTCCGATG AGGAAGAGGA GCAGAAGCCA CAGATTGCGG AAACCCAAGT GGATTGCGAG 
TCCGATGATT ACGAGAGAAT TCTTGACACG CAAATGCCTG ATCCTCGTAA GACTCCAACG 
AGACCTCGAC ACAAACGCAG TAAATTCAAT TCCTTTATTT TGGATGAGGC TGAAAAAAGC 
GGATCGGACC ATGAGGAGGA AGGGGAGACT ACCATAGGCA CATATCTGAA GGACTCCATG 
ATCGTTTCAA GCGATGATGA GGACCATAAC GACACAAACA CTCATGCTAT TTACCTTCGT 
GCACTCAAGA GCCCCATTCA AAGGCCAGGT GCCTTCAAGA TGCCCCCTCC ACGAGTTTTT 
CGCGACGAAT CGCACATATT TTCTCAGCCA GTGGAAGACG ATTCCTCGCA GTACATGCAA 
TGCTCATTTA TCGTTGATGA CGAAAGCTCC ACGATTGAAA GAGGGCATGA TGTCTCCGAA 
TGTCCTCTTG AGAAAGCGGA ACGTATTCTA AAGGAGCGAA GAAAGCAACG TCGACTAGGT 
AAGGTGCCTT CCGCTCCGGT TAATAAACGA CGCCGATTAC AAACCATAAG CACCTCTAGC 
GATGAAGATG ATGTTGTTTT AATTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650971.2
CDS	63..4532
Translation	MDVNWMDDDEDLVAALAMHEEQKSEDADGTRWEHCDEACDGFDMATGHNWIYPNNLPLRSYQQTIVQSALFKNTLVVLPTGLGKTFIAAVVMYNFYRWYPKGKIVFMAPTRPLVSQQIHASQKIMPFPSEDTVQLTGQLPRPKRAELWASKRVFFATPQVVHSDMLEADGECSFPFGSIKLIVVDEAHRAKGRYAYTQVADCLMARNRYFRMLALSATPGRTMEDVAAVCRNLYISNLQVRWDTSIDVQPYIHRRTIRTIVVSLKERIKEPRERLLQIIEPYLRQLMEAEIFKGNKGTVSRNSLLFEQKSFVERSAQGQRHPDHNIIMGNFAMCISMYHSLDLMERHGLRVFVNNFDADDDGREKFVLARDGNLRNLVEQVRQELGANPLDYTTHAMTNGEVPPLPSDLDFGHAKYEKLRQVLVQHFQANPDSRAIVFCEYRESVMLIHRLLLQHRPVLRPRCFVGQGSTVGASYALTQKQQLQIMTDFRSGTSNVLVATSIGEEGLDVGEVEMIVCFDICSTNPTRFIQRIGRTGRKKNGEVVMLVTEGREQQVLKDVLANKDQINKKLLNSSVVKLSLYEQNPRMVPSKFQPKCEEKHMEPVAEEKPKPKSAAKTKESRKRKQPVAQTGSLRKYFKESPPTESQQGILQGIKPYQMSEASQQLVKQQVLRRSVTLKNFFGESQASSTLTSSQEDVQRLRKLTRLLQSSKPFVSDSKDLMSHLQDDHLPRQIKLYLLKSNPEFLRETHLKMQVQSELNIADDRLNSRQRRTKNNYQLLLDICDGMDQMNELLQGDNSGAEISFRDELNPIYNQSPKKFDTICEQIFDGLNEHGLNSNNFELKQDLLEKLELRNLETTVNEQLGGIEASWSEEEWDQQEEDVKFESMYLSQQLNLPDANVVPHSSTPLRVKPLSKLMFKTLQGDIEEEYHSGMEASELGDNLGRLNSLMNASESKIKATERIAPIPVVDSTIETNHFPAVEESQRSTPISIADSSGESNHCAVNKNSEIYPMSIDETKVEVKSSLNLKMHAESIVEDLDIDLDDFLEPMDKELELASQTKGKSQENIVDHQMEKLEKNCEQKETAHNNDLDLTDEDLKEFLEPMVEEVELMSQKKANDFKDFLEPMPEELELMSQQKKNILLPSTETIKNSENKNSHEDGSRTVSPDIFGSDSMSPLKPQGKSLAAKLAAKAAAKVLPCPPPNSVGLNSPENRKRTNPSIQEKSPSIFDLYLNRMRGRGRLAKAAENLHRITSTNPTISVPKDEEDSPIARRPSKRKIVISSDEEEEQKPQIAETQVDCESDDYERILDTQMPDPRKTPTRPRHKRSKFNSFILDEAEKSGSDHEEEGETTIGTYLKDSMIVSSDDEDHNDTNTHAIYLRALKSPIQRPGAFKMPPPRVFRDESHIFSQPVEDDSSQYMQCSFIVDDESSTIERGHDVSECPLEKAERILKERRKQRRLGKVPSAPVNKRRRLQTISTSSDEDDVVLID

Target ORF information:

RefSeq Version	NM_142714.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fanconi anemia group M helicase, transcript variant A (Fancm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142714.3

ORF Insert Sequence:

ATGGATGTGA ATTGGATGGA CGATGACGAA GACCTAGTGG CGGCCTTGGC TATGCACGAA 
GAGCAGAAGA GCGAGGATGC CGATGGAACA CGTTGGGAAC ATTGCGACGA AGCCTGCGAT 
GGTTTCGATA TGGCCACAGG ACACAACTGG ATTTATCCAA ACAACCTGCC CCTACGATCC 
TATCAACAAA CCATCGTCCA ATCGGCGCTG TTCAAAAACA CCCTGGTGGT GTTGCCCACG 
GGATTGGGAA AGACCTTCAT TGCGGCGGTG GTTATGTACA ACTTTTACAG GTGGTATCCA 
AAGGGAAAGA TTGTGTTCAT GGCACCCACA AGGCCCCTGG TCTCGCAGCA GATCCACGCA 
TCCCAGAAGA TCATGCCCTT TCCATCTGAA GACACGGTGC AGCTAACAGG CCAACTTCCT 
CGACCCAAAC GAGCTGAGCT GTGGGCCTCC AAGCGGGTCT TCTTCGCTAC GCCGCAGGTG 
GTGCACTCAG ACATGCTGGA AGCTGATGGC GAATGCAGCT TTCCCTTTGG GTCCATCAAG 
TTGATAGTGG TGGATGAGGC GCACCGGGCC AAAGGTCGCT ATGCCTACAC CCAAGTGGCC 
GACTGTCTGA TGGCACGTAA CAGGTATTTC CGGATGCTTG CCCTGTCCGC TACACCAGGA 
AGAACCATGG AGGATGTGGC AGCTGTGTGC CGAAACCTAT ACATTTCCAA TCTGCAAGTG 
CGCTGGGACA CCTCGATCGA CGTGCAGCCC TACATACACA GGCGGACGAT TCGAACCATA 
GTGGTCTCCC TGAAGGAGAG AATCAAGGAG CCGAGAGAAC GATTGCTGCA GATCATCGAA 
CCATACCTAC GCCAGCTTAT GGAAGCTGAG ATTTTTAAAG GCAACAAGGG AACCGTGAGC 
AGAAATAGTT TGCTCTTTGA GCAGAAAAGC TTCGTCGAAC GATCGGCGCA AGGCCAGCGG 
CATCCGGATC ACAACATAAT AATGGGCAAC TTCGCCATGT GCATCAGCAT GTACCACTCG 
CTGGACCTAA TGGAGCGTCA CGGTCTTCGC GTCTTCGTGA ACAATTTCGA TGCAGACGAC 
GACGGGCGAG AGAAATTTGT ACTGGCCAGA GATGGTAATC TAAGGAATTT GGTGGAGCAG 
GTCCGCCAAG AACTCGGTGC CAATCCCCTA GATTACACCA CGCATGCCAT GACGAATGGA 
GAAGTGCCGC CTTTGCCATC TGATCTAGAC TTTGGACATG CCAAATATGA GAAGTTGCGA 
CAGGTGTTGG TGCAACACTT TCAGGCAAAC CCCGATTCCC GCGCCATTGT TTTCTGCGAG 
TACCGTGAAT CCGTAATGCT GATTCACCGG CTACTCCTTC AACACAGACC GGTGCTCCGT 
CCGCGCTGTT TTGTTGGCCA GGGCAGCACA GTTGGAGCAA GCTATGCTCT CACCCAGAAA 
CAGCAGCTGC AGATAATGAC CGACTTTCGA TCCGGCACTA GCAACGTTCT GGTGGCCACG 
TCGATCGGAG AGGAGGGCTT AGACGTCGGC GAGGTGGAGA TGATTGTGTG CTTCGACATT 
TGCAGCACTA ATCCCACTAG ATTTATTCAG CGGATTGGGC GCACTGGAAG GAAGAAGAAT 
GGAGAGGTGG TTATGCTAGT GACCGAAGGA CGAGAACAGC AGGTGCTTAA GGATGTTCTG 
GCCAACAAGG ATCAAATCAA CAAGAAGCTA TTGAATTCGT CCGTTGTTAA ATTGTCGCTT 
TATGAGCAAA ATCCTCGAAT GGTGCCATCA AAATTTCAAC CAAAATGCGA GGAGAAACAC 
ATGGAGCCAG TTGCTGAGGA GAAACCCAAG CCGAAGTCGG CAGCCAAGAC GAAAGAATCC 
AGGAAGCGGA AGCAACCAGT TGCACAGACT GGTAGCCTAA GGAAATACTT CAAAGAAAGT 
CCTCCAACGG AGAGTCAGCA AGGAATATTG CAGGGAATAA AACCCTACCA AATGAGCGAA 
GCTTCACAGC AATTGGTTAA GCAGCAAGTT CTACGGCGCA GCGTAACTCT GAAGAACTTC 
TTTGGGGAAT CTCAGGCCAG CTCAACGTTA ACCAGCAGTC AGGAAGATGT GCAACGCCTC 
CGAAAACTTA CTCGCCTTCT GCAGTCAAGC AAGCCATTTG TTTCGGACTC CAAGGATCTT 
ATGAGCCATC TGCAGGACGA TCACTTACCC AGGCAAATAA AGCTCTACTT ACTCAAAAGT 
AATCCCGAAT TTCTGAGGGA AACCCATTTG AAGATGCAAG TCCAGAGCGA GTTGAATATA 
GCTGATGATC GCTTAAATAG TCGGCAGCGA CGCACTAAAA ATAATTATCA ACTGCTTCTG 
GACATTTGCG ACGGAATGGA CCAGATGAAT GAGCTTCTTC AAGGGGATAA TAGTGGAGCA 
GAGATTAGCT TCAGGGATGA ACTAAATCCT ATTTATAATC AAAGTCCCAA AAAGTTCGAT 
ACCATTTGTG AACAGATTTT CGATGGTCTG AATGAGCATG GCCTAAACTC GAACAATTTT 
GAACTGAAGC AAGATCTTTT AGAGAAACTT GAGCTTCGCA ATCTGGAAAC CACAGTAAAC 
GAGCAACTGG GTGGCATTGA AGCTTCCTGG TCAGAAGAGG AGTGGGACCA ACAGGAGGAA 
GACGTGAAAT TTGAGTCTAT GTACCTAAGC CAGCAATTGA ACTTACCAGA CGCGAATGTT 
GTCCCCCATA GCTCAACGCC GCTCAGGGTC AAACCCTTGA GCAAATTAAT GTTTAAAACC 
CTGCAGGGCG ATATTGAAGA GGAGTATCAT TCCGGGATGG AAGCTAGTGA ATTGGGGGAC 
AACTTAGGTC GCCTAAATTC ACTGATGAAC GCAAGTGAAT CGAAGATTAA AGCGACTGAA 
AGAATTGCAC CTATTCCTGT AGTAGACTCT ACAATTGAGA CAAATCACTT TCCAGCCGTG 
GAAGAAAGTC AAAGATCAAC TCCTATTTCC ATTGCAGATT CTTCAGGTGA ATCAAATCAC 
TGTGCTGTTA ATAAAAATAG TGAGATATAT CCAATGAGCA TAGATGAGAC GAAAGTAGAA 
GTTAAGTCCT CTTTAAATCT GAAAATGCAC GCAGAATCCA TTGTAGAAGA TTTGGATATC 
GACTTGGATG ATTTTCTAGA GCCAATGGAC AAGGAACTGG AATTAGCCTC CCAGACAAAA 
GGGAAGTCGC AAGAGAACAT CGTAGATCAC CAGATGGAAA AATTGGAGAA AAACTGCGAG 
CAGAAAGAAA CAGCACACAA CAATGACCTT GATCTAACGG ACGAGGACTT GAAAGAATTT 
CTAGAGCCGA TGGTGGAGGA AGTGGAGTTG ATGAGCCAAA AAAAGGCGAA TGACTTCAAG 
GATTTTCTCG AGCCAATGCC AGAGGAGTTA GAGCTAATGA GCCAGCAGAA GAAAAACATA 
CTGCTTCCAA GTACAGAAAC AATTAAAAAC TCAGAAAATA AAAACTCACA TGAGGATGGC 
TCAAGAACCG TTTCGCCAGA TATCTTTGGA TCTGACTCCA TGTCACCTTT GAAGCCCCAA 
GGAAAAAGTC TGGCCGCAAA ATTGGCAGCC AAGGCAGCAG CAAAGGTTTT GCCTTGCCCA 
CCGCCGAATT CAGTAGGTCT TAATTCGCCG GAGAACCGGA AACGTACCAA TCCTTCTATC 
CAGGAAAAGT CTCCAAGTAT CTTTGATCTA TATTTAAATC GAATGCGGGG TCGTGGTCGT 
CTTGCTAAAG CTGCGGAGAA TCTTCACCGT ATAACTTCAA CGAATCCTAC AATCTCAGTA 
CCTAAAGACG AAGAGGATTC TCCTATTGCG CGGCGTCCAT CCAAACGAAA GATTGTTATA 
AGTTCCGATG AGGAAGAGGA GCAGAAGCCA CAGATTGCGG AAACCCAAGT GGATTGCGAG 
TCCGATGATT ACGAGAGAAT TCTTGACACG CAAATGCCTG ATCCTCGTAA GACTCCAACG 
AGACCTCGAC ACAAACGCAG TAAATTCAAT TCCTTTATTT TGGATGAGGC TGAAAAAAGC 
GGATCGGACC ATGAGGAGGA AGGGGAGACT ACCATAGGCA CATATCTGAA GGACTCCATG 
ATCGTTTCAA GCGATGATGA GGACCATAAC GACACAAACA CTCATGCTAT TTACCTTCGT 
GCACTCAAGA GCCCCATTCA AAGGCCAGGT GCCTTCAAGA TGCCCCCTCC ACGAGTTTTT 
CGCGACGAAT CGCACATATT TTCTCAGCCA GTGGAAGACG ATTCCTCGCA GTACATGCAA 
TGCTCATTTA TCGTTGATGA CGAAAGCTCC ACGATTGAAA GAGGGCATGA TGTCTCCGAA 
TGTCCTCTTG AGAAAGCGGA ACGTATTCTA AAGGAGCGAA GAAAGCAACG TCGACTAGGT 
AAGGTGCCTT CCGCTCCGGT TAATAAACGA CGCCGATTAC AAACCATAAG CACCTCTAGC 
GATGAAGATG ATGTTGTTTT AATTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fancm ( NM_001275877.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262806.1 Drosophila melanogaster fanconi anemia group M helicase, transcript variant B (Fancm), mRNA.

Price & Availability↑

CloneID	OFa05603
Clone ID Related Accession (Same CDS sequence)	NM_142714.3 , NM_001275877.1
Accession Version	NM_001275877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fanconi anemia group M helicase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGTGA ATTGGATGGA CGATGACGAA GACCTAGTGG CGGCCTTGGC TATGCACGAA 
GAGCAGAAGA GCGAGGATGC CGATGGAACA CGTTGGGAAC ATTGCGACGA AGCCTGCGAT 
GGTTTCGATA TGGCCACAGG ACACAACTGG ATTTATCCAA ACAACCTGCC CCTACGATCC 
TATCAACAAA CCATCGTCCA ATCGGCGCTG TTCAAAAACA CCCTGGTGGT GTTGCCCACG 
GGATTGGGAA AGACCTTCAT TGCGGCGGTG GTTATGTACA ACTTTTACAG GTGGTATCCA 
AAGGGAAAGA TTGTGTTCAT GGCACCCACA AGGCCCCTGG TCTCGCAGCA GATCCACGCA 
TCCCAGAAGA TCATGCCCTT TCCATCTGAA GACACGGTGC AGCTAACAGG CCAACTTCCT 
CGACCCAAAC GAGCTGAGCT GTGGGCCTCC AAGCGGGTCT TCTTCGCTAC GCCGCAGGTG 
GTGCACTCAG ACATGCTGGA AGCTGATGGC GAATGCAGCT TTCCCTTTGG GTCCATCAAG 
TTGATAGTGG TGGATGAGGC GCACCGGGCC AAAGGTCGCT ATGCCTACAC CCAAGTGGCC 
GACTGTCTGA TGGCACGTAA CAGGTATTTC CGGATGCTTG CCCTGTCCGC TACACCAGGA 
AGAACCATGG AGGATGTGGC AGCTGTGTGC CGAAACCTAT ACATTTCCAA TCTGCAAGTG 
CGCTGGGACA CCTCGATCGA CGTGCAGCCC TACATACACA GGCGGACGAT TCGAACCATA 
GTGGTCTCCC TGAAGGAGAG AATCAAGGAG CCGAGAGAAC GATTGCTGCA GATCATCGAA 
CCATACCTAC GCCAGCTTAT GGAAGCTGAG ATTTTTAAAG GCAACAAGGG AACCGTGAGC 
AGAAATAGTT TGCTCTTTGA GCAGAAAAGC TTCGTCGAAC GATCGGCGCA AGGCCAGCGG 
CATCCGGATC ACAACATAAT AATGGGCAAC TTCGCCATGT GCATCAGCAT GTACCACTCG 
CTGGACCTAA TGGAGCGTCA CGGTCTTCGC GTCTTCGTGA ACAATTTCGA TGCAGACGAC 
GACGGGCGAG AGAAATTTGT ACTGGCCAGA GATGGTAATC TAAGGAATTT GGTGGAGCAG 
GTCCGCCAAG AACTCGGTGC CAATCCCCTA GATTACACCA CGCATGCCAT GACGAATGGA 
GAAGTGCCGC CTTTGCCATC TGATCTAGAC TTTGGACATG CCAAATATGA GAAGTTGCGA 
CAGGTGTTGG TGCAACACTT TCAGGCAAAC CCCGATTCCC GCGCCATTGT TTTCTGCGAG 
TACCGTGAAT CCGTAATGCT GATTCACCGG CTACTCCTTC AACACAGACC GGTGCTCCGT 
CCGCGCTGTT TTGTTGGCCA GGGCAGCACA GTTGGAGCAA GCTATGCTCT CACCCAGAAA 
CAGCAGCTGC AGATAATGAC CGACTTTCGA TCCGGCACTA GCAACGTTCT GGTGGCCACG 
TCGATCGGAG AGGAGGGCTT AGACGTCGGC GAGGTGGAGA TGATTGTGTG CTTCGACATT 
TGCAGCACTA ATCCCACTAG ATTTATTCAG CGGATTGGGC GCACTGGAAG GAAGAAGAAT 
GGAGAGGTGG TTATGCTAGT GACCGAAGGA CGAGAACAGC AGGTGCTTAA GGATGTTCTG 
GCCAACAAGG ATCAAATCAA CAAGAAGCTA TTGAATTCGT CCGTTGTTAA ATTGTCGCTT 
TATGAGCAAA ATCCTCGAAT GGTGCCATCA AAATTTCAAC CAAAATGCGA GGAGAAACAC 
ATGGAGCCAG TTGCTGAGGA GAAACCCAAG CCGAAGTCGG CAGCCAAGAC GAAAGAATCC 
AGGAAGCGGA AGCAACCAGT TGCACAGACT GGTAGCCTAA GGAAATACTT CAAAGAAAGT 
CCTCCAACGG AGAGTCAGCA AGGAATATTG CAGGGAATAA AACCCTACCA AATGAGCGAA 
GCTTCACAGC AATTGGTTAA GCAGCAAGTT CTACGGCGCA GCGTAACTCT GAAGAACTTC 
TTTGGGGAAT CTCAGGCCAG CTCAACGTTA ACCAGCAGTC AGGAAGATGT GCAACGCCTC 
CGAAAACTTA CTCGCCTTCT GCAGTCAAGC AAGCCATTTG TTTCGGACTC CAAGGATCTT 
ATGAGCCATC TGCAGGACGA TCACTTACCC AGGCAAATAA AGCTCTACTT ACTCAAAAGT 
AATCCCGAAT TTCTGAGGGA AACCCATTTG AAGATGCAAG TCCAGAGCGA GTTGAATATA 
GCTGATGATC GCTTAAATAG TCGGCAGCGA CGCACTAAAA ATAATTATCA ACTGCTTCTG 
GACATTTGCG ACGGAATGGA CCAGATGAAT GAGCTTCTTC AAGGGGATAA TAGTGGAGCA 
GAGATTAGCT TCAGGGATGA ACTAAATCCT ATTTATAATC AAAGTCCCAA AAAGTTCGAT 
ACCATTTGTG AACAGATTTT CGATGGTCTG AATGAGCATG GCCTAAACTC GAACAATTTT 
GAACTGAAGC AAGATCTTTT AGAGAAACTT GAGCTTCGCA ATCTGGAAAC CACAGTAAAC 
GAGCAACTGG GTGGCATTGA AGCTTCCTGG TCAGAAGAGG AGTGGGACCA ACAGGAGGAA 
GACGTGAAAT TTGAGTCTAT GTACCTAAGC CAGCAATTGA ACTTACCAGA CGCGAATGTT 
GTCCCCCATA GCTCAACGCC GCTCAGGGTC AAACCCTTGA GCAAATTAAT GTTTAAAACC 
CTGCAGGGCG ATATTGAAGA GGAGTATCAT TCCGGGATGG AAGCTAGTGA ATTGGGGGAC 
AACTTAGGTC GCCTAAATTC ACTGATGAAC GCAAGTGAAT CGAAGATTAA AGCGACTGAA 
AGAATTGCAC CTATTCCTGT AGTAGACTCT ACAATTGAGA CAAATCACTT TCCAGCCGTG 
GAAGAAAGTC AAAGATCAAC TCCTATTTCC ATTGCAGATT CTTCAGGTGA ATCAAATCAC 
TGTGCTGTTA ATAAAAATAG TGAGATATAT CCAATGAGCA TAGATGAGAC GAAAGTAGAA 
GTTAAGTCCT CTTTAAATCT GAAAATGCAC GCAGAATCCA TTGTAGAAGA TTTGGATATC 
GACTTGGATG ATTTTCTAGA GCCAATGGAC AAGGAACTGG AATTAGCCTC CCAGACAAAA 
GGGAAGTCGC AAGAGAACAT CGTAGATCAC CAGATGGAAA AATTGGAGAA AAACTGCGAG 
CAGAAAGAAA CAGCACACAA CAATGACCTT GATCTAACGG ACGAGGACTT GAAAGAATTT 
CTAGAGCCGA TGGTGGAGGA AGTGGAGTTG ATGAGCCAAA AAAAGGCGAA TGACTTCAAG 
GATTTTCTCG AGCCAATGCC AGAGGAGTTA GAGCTAATGA GCCAGCAGAA GAAAAACATA 
CTGCTTCCAA GTACAGAAAC AATTAAAAAC TCAGAAAATA AAAACTCACA TGAGGATGGC 
TCAAGAACCG TTTCGCCAGA TATCTTTGGA TCTGACTCCA TGTCACCTTT GAAGCCCCAA 
GGAAAAAGTC TGGCCGCAAA ATTGGCAGCC AAGGCAGCAG CAAAGGTTTT GCCTTGCCCA 
CCGCCGAATT CAGTAGGTCT TAATTCGCCG GAGAACCGGA AACGTACCAA TCCTTCTATC 
CAGGAAAAGT CTCCAAGTAT CTTTGATCTA TATTTAAATC GAATGCGGGG TCGTGGTCGT 
CTTGCTAAAG CTGCGGAGAA TCTTCACCGT ATAACTTCAA CGAATCCTAC AATCTCAGTA 
CCTAAAGACG AAGAGGATTC TCCTATTGCG CGGCGTCCAT CCAAACGAAA GATTGTTATA 
AGTTCCGATG AGGAAGAGGA GCAGAAGCCA CAGATTGCGG AAACCCAAGT GGATTGCGAG 
TCCGATGATT ACGAGAGAAT TCTTGACACG CAAATGCCTG ATCCTCGTAA GACTCCAACG 
AGACCTCGAC ACAAACGCAG TAAATTCAAT TCCTTTATTT TGGATGAGGC TGAAAAAAGC 
GGATCGGACC ATGAGGAGGA AGGGGAGACT ACCATAGGCA CATATCTGAA GGACTCCATG 
ATCGTTTCAA GCGATGATGA GGACCATAAC GACACAAACA CTCATGCTAT TTACCTTCGT 
GCACTCAAGA GCCCCATTCA AAGGCCAGGT GCCTTCAAGA TGCCCCCTCC ACGAGTTTTT 
CGCGACGAAT CGCACATATT TTCTCAGCCA GTGGAAGACG ATTCCTCGCA GTACATGCAA 
TGCTCATTTA TCGTTGATGA CGAAAGCTCC ACGATTGAAA GAGGGCATGA TGTCTCCGAA 
TGTCCTCTTG AGAAAGCGGA ACGTATTCTA AAGGAGCGAA GAAAGCAACG TCGACTAGGT 
AAGGTGCCTT CCGCTCCGGT TAATAAACGA CGCCGATTAC AAACCATAAG CACCTCTAGC 
GATGAAGATG ATGTTGTTTT AATTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262806.1
CDS	63..4532
Translation	MDVNWMDDDEDLVAALAMHEEQKSEDADGTRWEHCDEACDGFDMATGHNWIYPNNLPLRSYQQTIVQSALFKNTLVVLPTGLGKTFIAAVVMYNFYRWYPKGKIVFMAPTRPLVSQQIHASQKIMPFPSEDTVQLTGQLPRPKRAELWASKRVFFATPQVVHSDMLEADGECSFPFGSIKLIVVDEAHRAKGRYAYTQVADCLMARNRYFRMLALSATPGRTMEDVAAVCRNLYISNLQVRWDTSIDVQPYIHRRTIRTIVVSLKERIKEPRERLLQIIEPYLRQLMEAEIFKGNKGTVSRNSLLFEQKSFVERSAQGQRHPDHNIIMGNFAMCISMYHSLDLMERHGLRVFVNNFDADDDGREKFVLARDGNLRNLVEQVRQELGANPLDYTTHAMTNGEVPPLPSDLDFGHAKYEKLRQVLVQHFQANPDSRAIVFCEYRESVMLIHRLLLQHRPVLRPRCFVGQGSTVGASYALTQKQQLQIMTDFRSGTSNVLVATSIGEEGLDVGEVEMIVCFDICSTNPTRFIQRIGRTGRKKNGEVVMLVTEGREQQVLKDVLANKDQINKKLLNSSVVKLSLYEQNPRMVPSKFQPKCEEKHMEPVAEEKPKPKSAAKTKESRKRKQPVAQTGSLRKYFKESPPTESQQGILQGIKPYQMSEASQQLVKQQVLRRSVTLKNFFGESQASSTLTSSQEDVQRLRKLTRLLQSSKPFVSDSKDLMSHLQDDHLPRQIKLYLLKSNPEFLRETHLKMQVQSELNIADDRLNSRQRRTKNNYQLLLDICDGMDQMNELLQGDNSGAEISFRDELNPIYNQSPKKFDTICEQIFDGLNEHGLNSNNFELKQDLLEKLELRNLETTVNEQLGGIEASWSEEEWDQQEEDVKFESMYLSQQLNLPDANVVPHSSTPLRVKPLSKLMFKTLQGDIEEEYHSGMEASELGDNLGRLNSLMNASESKIKATERIAPIPVVDSTIETNHFPAVEESQRSTPISIADSSGESNHCAVNKNSEIYPMSIDETKVEVKSSLNLKMHAESIVEDLDIDLDDFLEPMDKELELASQTKGKSQENIVDHQMEKLEKNCEQKETAHNNDLDLTDEDLKEFLEPMVEEVELMSQKKANDFKDFLEPMPEELELMSQQKKNILLPSTETIKNSENKNSHEDGSRTVSPDIFGSDSMSPLKPQGKSLAAKLAAKAAAKVLPCPPPNSVGLNSPENRKRTNPSIQEKSPSIFDLYLNRMRGRGRLAKAAENLHRITSTNPTISVPKDEEDSPIARRPSKRKIVISSDEEEEQKPQIAETQVDCESDDYERILDTQMPDPRKTPTRPRHKRSKFNSFILDEAEKSGSDHEEEGETTIGTYLKDSMIVSSDDEDHNDTNTHAIYLRALKSPIQRPGAFKMPPPRVFRDESHIFSQPVEDDSSQYMQCSFIVDDESSTIERGHDVSECPLEKAERILKERRKQRRLGKVPSAPVNKRRRLQTISTSSDEDDVVLID

Target ORF information:

RefSeq Version	NM_001275877.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fanconi anemia group M helicase, transcript variant B (Fancm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275877.1

ORF Insert Sequence:

ATGGATGTGA ATTGGATGGA CGATGACGAA GACCTAGTGG CGGCCTTGGC TATGCACGAA 
GAGCAGAAGA GCGAGGATGC CGATGGAACA CGTTGGGAAC ATTGCGACGA AGCCTGCGAT 
GGTTTCGATA TGGCCACAGG ACACAACTGG ATTTATCCAA ACAACCTGCC CCTACGATCC 
TATCAACAAA CCATCGTCCA ATCGGCGCTG TTCAAAAACA CCCTGGTGGT GTTGCCCACG 
GGATTGGGAA AGACCTTCAT TGCGGCGGTG GTTATGTACA ACTTTTACAG GTGGTATCCA 
AAGGGAAAGA TTGTGTTCAT GGCACCCACA AGGCCCCTGG TCTCGCAGCA GATCCACGCA 
TCCCAGAAGA TCATGCCCTT TCCATCTGAA GACACGGTGC AGCTAACAGG CCAACTTCCT 
CGACCCAAAC GAGCTGAGCT GTGGGCCTCC AAGCGGGTCT TCTTCGCTAC GCCGCAGGTG 
GTGCACTCAG ACATGCTGGA AGCTGATGGC GAATGCAGCT TTCCCTTTGG GTCCATCAAG 
TTGATAGTGG TGGATGAGGC GCACCGGGCC AAAGGTCGCT ATGCCTACAC CCAAGTGGCC 
GACTGTCTGA TGGCACGTAA CAGGTATTTC CGGATGCTTG CCCTGTCCGC TACACCAGGA 
AGAACCATGG AGGATGTGGC AGCTGTGTGC CGAAACCTAT ACATTTCCAA TCTGCAAGTG 
CGCTGGGACA CCTCGATCGA CGTGCAGCCC TACATACACA GGCGGACGAT TCGAACCATA 
GTGGTCTCCC TGAAGGAGAG AATCAAGGAG CCGAGAGAAC GATTGCTGCA GATCATCGAA 
CCATACCTAC GCCAGCTTAT GGAAGCTGAG ATTTTTAAAG GCAACAAGGG AACCGTGAGC 
AGAAATAGTT TGCTCTTTGA GCAGAAAAGC TTCGTCGAAC GATCGGCGCA AGGCCAGCGG 
CATCCGGATC ACAACATAAT AATGGGCAAC TTCGCCATGT GCATCAGCAT GTACCACTCG 
CTGGACCTAA TGGAGCGTCA CGGTCTTCGC GTCTTCGTGA ACAATTTCGA TGCAGACGAC 
GACGGGCGAG AGAAATTTGT ACTGGCCAGA GATGGTAATC TAAGGAATTT GGTGGAGCAG 
GTCCGCCAAG AACTCGGTGC CAATCCCCTA GATTACACCA CGCATGCCAT GACGAATGGA 
GAAGTGCCGC CTTTGCCATC TGATCTAGAC TTTGGACATG CCAAATATGA GAAGTTGCGA 
CAGGTGTTGG TGCAACACTT TCAGGCAAAC CCCGATTCCC GCGCCATTGT TTTCTGCGAG 
TACCGTGAAT CCGTAATGCT GATTCACCGG CTACTCCTTC AACACAGACC GGTGCTCCGT 
CCGCGCTGTT TTGTTGGCCA GGGCAGCACA GTTGGAGCAA GCTATGCTCT CACCCAGAAA 
CAGCAGCTGC AGATAATGAC CGACTTTCGA TCCGGCACTA GCAACGTTCT GGTGGCCACG 
TCGATCGGAG AGGAGGGCTT AGACGTCGGC GAGGTGGAGA TGATTGTGTG CTTCGACATT 
TGCAGCACTA ATCCCACTAG ATTTATTCAG CGGATTGGGC GCACTGGAAG GAAGAAGAAT 
GGAGAGGTGG TTATGCTAGT GACCGAAGGA CGAGAACAGC AGGTGCTTAA GGATGTTCTG 
GCCAACAAGG ATCAAATCAA CAAGAAGCTA TTGAATTCGT CCGTTGTTAA ATTGTCGCTT 
TATGAGCAAA ATCCTCGAAT GGTGCCATCA AAATTTCAAC CAAAATGCGA GGAGAAACAC 
ATGGAGCCAG TTGCTGAGGA GAAACCCAAG CCGAAGTCGG CAGCCAAGAC GAAAGAATCC 
AGGAAGCGGA AGCAACCAGT TGCACAGACT GGTAGCCTAA GGAAATACTT CAAAGAAAGT 
CCTCCAACGG AGAGTCAGCA AGGAATATTG CAGGGAATAA AACCCTACCA AATGAGCGAA 
GCTTCACAGC AATTGGTTAA GCAGCAAGTT CTACGGCGCA GCGTAACTCT GAAGAACTTC 
TTTGGGGAAT CTCAGGCCAG CTCAACGTTA ACCAGCAGTC AGGAAGATGT GCAACGCCTC 
CGAAAACTTA CTCGCCTTCT GCAGTCAAGC AAGCCATTTG TTTCGGACTC CAAGGATCTT 
ATGAGCCATC TGCAGGACGA TCACTTACCC AGGCAAATAA AGCTCTACTT ACTCAAAAGT 
AATCCCGAAT TTCTGAGGGA AACCCATTTG AAGATGCAAG TCCAGAGCGA GTTGAATATA 
GCTGATGATC GCTTAAATAG TCGGCAGCGA CGCACTAAAA ATAATTATCA ACTGCTTCTG 
GACATTTGCG ACGGAATGGA CCAGATGAAT GAGCTTCTTC AAGGGGATAA TAGTGGAGCA 
GAGATTAGCT TCAGGGATGA ACTAAATCCT ATTTATAATC AAAGTCCCAA AAAGTTCGAT 
ACCATTTGTG AACAGATTTT CGATGGTCTG AATGAGCATG GCCTAAACTC GAACAATTTT 
GAACTGAAGC AAGATCTTTT AGAGAAACTT GAGCTTCGCA ATCTGGAAAC CACAGTAAAC 
GAGCAACTGG GTGGCATTGA AGCTTCCTGG TCAGAAGAGG AGTGGGACCA ACAGGAGGAA 
GACGTGAAAT TTGAGTCTAT GTACCTAAGC CAGCAATTGA ACTTACCAGA CGCGAATGTT 
GTCCCCCATA GCTCAACGCC GCTCAGGGTC AAACCCTTGA GCAAATTAAT GTTTAAAACC 
CTGCAGGGCG ATATTGAAGA GGAGTATCAT TCCGGGATGG AAGCTAGTGA ATTGGGGGAC 
AACTTAGGTC GCCTAAATTC ACTGATGAAC GCAAGTGAAT CGAAGATTAA AGCGACTGAA 
AGAATTGCAC CTATTCCTGT AGTAGACTCT ACAATTGAGA CAAATCACTT TCCAGCCGTG 
GAAGAAAGTC AAAGATCAAC TCCTATTTCC ATTGCAGATT CTTCAGGTGA ATCAAATCAC 
TGTGCTGTTA ATAAAAATAG TGAGATATAT CCAATGAGCA TAGATGAGAC GAAAGTAGAA 
GTTAAGTCCT CTTTAAATCT GAAAATGCAC GCAGAATCCA TTGTAGAAGA TTTGGATATC 
GACTTGGATG ATTTTCTAGA GCCAATGGAC AAGGAACTGG AATTAGCCTC CCAGACAAAA 
GGGAAGTCGC AAGAGAACAT CGTAGATCAC CAGATGGAAA AATTGGAGAA AAACTGCGAG 
CAGAAAGAAA CAGCACACAA CAATGACCTT GATCTAACGG ACGAGGACTT GAAAGAATTT 
CTAGAGCCGA TGGTGGAGGA AGTGGAGTTG ATGAGCCAAA AAAAGGCGAA TGACTTCAAG 
GATTTTCTCG AGCCAATGCC AGAGGAGTTA GAGCTAATGA GCCAGCAGAA GAAAAACATA 
CTGCTTCCAA GTACAGAAAC AATTAAAAAC TCAGAAAATA AAAACTCACA TGAGGATGGC 
TCAAGAACCG TTTCGCCAGA TATCTTTGGA TCTGACTCCA TGTCACCTTT GAAGCCCCAA 
GGAAAAAGTC TGGCCGCAAA ATTGGCAGCC AAGGCAGCAG CAAAGGTTTT GCCTTGCCCA 
CCGCCGAATT CAGTAGGTCT TAATTCGCCG GAGAACCGGA AACGTACCAA TCCTTCTATC 
CAGGAAAAGT CTCCAAGTAT CTTTGATCTA TATTTAAATC GAATGCGGGG TCGTGGTCGT 
CTTGCTAAAG CTGCGGAGAA TCTTCACCGT ATAACTTCAA CGAATCCTAC AATCTCAGTA 
CCTAAAGACG AAGAGGATTC TCCTATTGCG CGGCGTCCAT CCAAACGAAA GATTGTTATA 
AGTTCCGATG AGGAAGAGGA GCAGAAGCCA CAGATTGCGG AAACCCAAGT GGATTGCGAG 
TCCGATGATT ACGAGAGAAT TCTTGACACG CAAATGCCTG ATCCTCGTAA GACTCCAACG 
AGACCTCGAC ACAAACGCAG TAAATTCAAT TCCTTTATTT TGGATGAGGC TGAAAAAAGC 
GGATCGGACC ATGAGGAGGA AGGGGAGACT ACCATAGGCA CATATCTGAA GGACTCCATG 
ATCGTTTCAA GCGATGATGA GGACCATAAC GACACAAACA CTCATGCTAT TTACCTTCGT 
GCACTCAAGA GCCCCATTCA AAGGCCAGGT GCCTTCAAGA TGCCCCCTCC ACGAGTTTTT 
CGCGACGAAT CGCACATATT TTCTCAGCCA GTGGAAGACG ATTCCTCGCA GTACATGCAA 
TGCTCATTTA TCGTTGATGA CGAAAGCTCC ACGATTGAAA GAGGGCATGA TGTCTCCGAA 
TGTCCTCTTG AGAAAGCGGA ACGTATTCTA AAGGAGCGAA GAAAGCAACG TCGACTAGGT 
AAGGTGCCTT CCGCTCCGGT TAATAAACGA CGCCGATTAC AAACCATAAG CACCTCTAGC 
GATGAAGATG ATGTTGTTTT AATTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fancm cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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