VhaAC39-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	VhaAC39-2
Entrez Gene ID	42739
Full Name	Vacuolar H[+] ATPase AC39 subunit 2
Synonyms	CG4624,Dmel\CG4624,V0d,anon-WO0118547.227,vhaAC39-2
General protein information	Preferred Names Vacuolar H[+] ATPase AC39 subunit 2 Names CG4624 gene product from transcript CG4624-RA CG4624-PA VhaAC39-2-PA vacuolar H[+] ATPase AC39 subunit 2 vacuolar H[+] ATPase subunit AC39-2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94E2-94E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_142871.1	NP_651128.1	vacuolar H[+] ATPase AC39 subunit 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VhaAC39-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VhaAC39-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05756	NM_142871.1 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase AC39 subunit 2 (VhaAC39-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VhaAC39-2 ( NM_142871.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651128.1 Drosophila melanogaster vacuolar H[+] ATPase AC39 subunit 2 (VhaAC39-2), mRNA.

Price & Availability↑

CloneID	OFa05756
Clone ID Related Accession (Same CDS sequence)	NM_142871.1
Accession Version	NM_142871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase AC39 subunit 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGATGA TATTCAACAC GGAGTATGGC TACCTGGAGG CCCTAACGCG CGGATTCAAG 
AACGGCATGC TAAAGCACTC GGACTATCTA AATCTGACCC AGTGTGAATC CCTCGAGGAC 
GTAATGATCA GCATCCAGGG CACGGATTAC GGTCTCATCT TTGGTGGCGA ACAATCAGCG 
CCGTCCGTTG AGGTGATCGA AAGATGCCTT AGGGATCGTC TGCTGCAGCA ATACTACTAC 
ATACGCAGTC ATTCGACGGA GCCGCTGACC ACCTTTATGG AGTTCATACG ATATCCGTTC 
ATGATCGATA ATGTGGCGCT TCTGGTCGCT GGCCTAAACA ATCACCGATC CATGAAGCGT 
CTGCTAAGAA TGTGTCATCC GCTGGGCGAA TTCGATCAAC TGGGCGCCAT CGAAGTGGCC 
TCGAATTCTG CGGAGCTCTT CGACGCCGTT CTAATTGACA CGCCCATCGC TCGGTTTGTG 
CCACGCGATT TGCCCATGGA GTCGTTGCGT TACCTCGACG TGGAGATTGT GCGTGCCCAT 
CTGTATCGTG CTTATTTGGA GAAATTCTAC GCCTATTGCA GCCAACTGGG CGGCAATACC 
GCCAACGTGA TGACCAATCT ATTGTCCTTC GAGGCGGATC GTCGGACCAT TACAATAGCT 
GTGAACGCTA TTGGCAGTGA TATCATACCG AAGGAGCGTC TAAAGATGTT CCCCACCTGT 
GGCTACCTGC CCAAAATAGC CTTGGCCTCC ATGTCCACTT TAAATGATAC GGATAAAATA 
AGGGATGTTT GCAATGTGTT CGATGGCTAT GGCAAAATGT TTGACAATTT GGAGCGCGAC 
TCGGACGGCA TGATTACGCT GGAGGACCGC TTTCTCATGA TGGAGGCCAA GAAGAATGTG 
CAGACCTTCC TGCAGCAGTA CCATTTCGGG ATCTTCTACT CGTTCATTAA ACTTAAACAG 
TTGGAGGTAC GCAATATCGT CTGGATTAGC GAATGCATAG CGCAGCGACA GACGGACAGA 
ATCAACGCCT TCATTCCCAT ACCCTTGGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651128.1
CDS	78..1130
Translation	MSMIFNTEYGYLEALTRGFKNGMLKHSDYLNLTQCESLEDVMISIQGTDYGLIFGGEQSAPSVEVIERCLRDRLLQQYYYIRSHSTEPLTTFMEFIRYPFMIDNVALLVAGLNNHRSMKRLLRMCHPLGEFDQLGAIEVASNSAELFDAVLIDTPIARFVPRDLPMESLRYLDVEIVRAHLYRAYLEKFYAYCSQLGGNTANVMTNLLSFEADRRTITIAVNAIGSDIIPKERLKMFPTCGYLPKIALASMSTLNDTDKIRDVCNVFDGYGKMFDNLERDSDGMITLEDRFLMMEAKKNVQTFLQQYHFGIFYSFIKLKQLEVRNIVWISECIAQRQTDRINAFIPIPLD

Target ORF information:

RefSeq Version	NM_142871.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase AC39 subunit 2 (VhaAC39-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142871.1

ORF Insert Sequence:

ATGTCGATGA TATTCAACAC GGAGTATGGC TACCTGGAGG CCCTAACGCG CGGATTCAAG 
AACGGCATGC TAAAGCACTC GGACTATCTA AATCTGACCC AGTGTGAATC CCTCGAGGAC 
GTAATGATCA GCATCCAGGG CACGGATTAC GGTCTCATCT TTGGTGGCGA ACAATCAGCG 
CCGTCCGTTG AGGTGATCGA AAGATGCCTT AGGGATCGTC TGCTGCAGCA ATACTACTAC 
ATACGCAGTC ATTCGACGGA GCCGCTGACC ACCTTTATGG AGTTCATACG ATATCCGTTC 
ATGATCGATA ATGTGGCGCT TCTGGTCGCT GGCCTAAACA ATCACCGATC CATGAAGCGT 
CTGCTAAGAA TGTGTCATCC GCTGGGCGAA TTCGATCAAC TGGGCGCCAT CGAAGTGGCC 
TCGAATTCTG CGGAGCTCTT CGACGCCGTT CTAATTGACA CGCCCATCGC TCGGTTTGTG 
CCACGCGATT TGCCCATGGA GTCGTTGCGT TACCTCGACG TGGAGATTGT GCGTGCCCAT 
CTGTATCGTG CTTATTTGGA GAAATTCTAC GCCTATTGCA GCCAACTGGG CGGCAATACC 
GCCAACGTGA TGACCAATCT ATTGTCCTTC GAGGCGGATC GTCGGACCAT TACAATAGCT 
GTGAACGCTA TTGGCAGTGA TATCATACCG AAGGAGCGTC TAAAGATGTT CCCCACCTGT 
GGCTACCTGC CCAAAATAGC CTTGGCCTCC ATGTCCACTT TAAATGATAC GGATAAAATA 
AGGGATGTTT GCAATGTGTT CGATGGCTAT GGCAAAATGT TTGACAATTT GGAGCGCGAC 
TCGGACGGCA TGATTACGCT GGAGGACCGC TTTCTCATGA TGGAGGCCAA GAAGAATGTG 
CAGACCTTCC TGCAGCAGTA CCATTTCGGG ATCTTCTACT CGTTCATTAA ACTTAAACAG 
TTGGAGGTAC GCAATATCGT CTGGATTAGC GAATGCATAG CGCAGCGACA GACGGACAGA 
ATCAACGCCT TCATTCCCAT ACCCTTGGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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VhaAC39-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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