Nup358 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nup358
Entrez Gene ID	43041
Full Name	Nucleoporin 358kD
Synonyms	CG11856,Dmel\CG11856,NUP358,Q9VBU7_DROME,RanBP2,nup358
General protein information	Preferred Names Nucleoporin 358kD Names CG11856 gene product from transcript CG11856-RB CG11856-PA CG11856-PB Nup358-PA Nup358-PB nucleoporin 358 nucleoporin 358kD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96C8-96C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260373.2	NP_001247302.1	nucleoporin 358kD, isoform B
NM_143104.3	NP_651361.2	nucleoporin 358kD, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

facilitates JH-induced Met nuclear transport in a manner dependent on importin beta and Hsp83
Title: Nucleoporin Nup358 facilitates nuclear import of Methoprene-tolerant (Met) in an importin ?- and Hsp83-dependent manner.

The following Nup358 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup358 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06067	NM_001260373.2 Latest version!	Drosophila melanogaster nucleoporin 358kD, transcript variant B (Nup358), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa08829	NM_143104.3 Latest version!	Drosophila melanogaster nucleoporin 358kD, transcript variant A (Nup358), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nup358 ( NM_001260373.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247302.1 Drosophila melanogaster nucleoporin 358kD, transcript variant B (Nup358), mRNA.

Price & Availability↑

CloneID	OFa06067
Clone ID Related Accession (Same CDS sequence)	NM_001260373.2
Accession Version	NM_001260373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 358kD, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260373.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTACAA CGCGAAAAGA AGTGGACGCA CACGTCCACA AAATGTTGGG AAAACTTCAG 
CCGGGAAGAG AGCGCGATAT CAAAGGCTTG GCTGTAGCTC GATTGTACAT GAAAGTTCAG 
GAATACCCAA AAGCCATCGA GTATTTGAAC GGCTATCTCC GGGTGAGGGA CGACGCTGTA 
GGCCATAACA TGATTGCCAC TTGCTACAGT AGGCTGAACC CGCCCGATGT GACAGAGGCC 
CTGCAGCACT ACCAACGTTC TATTCAAATT GATCCGCGTC AGTCAGAGGT TGTCATTGAC 
GCCTGCGAAC TTCTGGTGAA GGAGAACAAC GCATCGATCA CGGAGTGCGC CAGGTACTGG 
TTGGATCAGG CTAATAGCCT TGATTTAAGC GGCAACAAGC AAGTCTTCAA CCTGCGAATG 
CGTGTAAATC TTGCGGACAG TAACGGCGAA AGAGATGACA CCAGTGGCGG AGACGGCGAG 
CAAAATACCC TCGAGATTCT TATGTACAAG GAGCTGCAGG CGCGTCCCCA GGATGTTAAT 
ATACGCATAC AACTGCTGCG CAGCTATGTT GAAAAAATGA AAATAGACCA GGCCTTCAAC 
TATGCTCTAA AAACGGAACT GGAGTCGAAA AACTGCACGA GCCAGTCGAA CGAATGGTAC 
GAGCAGATCT GGATGGTCCT TTTCAAGATA GAAATGGCCA AGGATGTAAA GAAGAACTGG 
CGATTTTGGC ACTTCGCCTT GCACACTCTC GATCGTCTCG TGCAGCTAAG CCTGGAGGGC 
AGTGGACTGG CAGACAGCAG CAAGCAGTTG TTCAGACTTG ATCAATATCT ATTCAAGTTT 
AGCACTTCGA TAGAGAGGTC AGGGGACGCT CCGCAGCGGG ATCTCCACCA GGCTTGTATT 
GACCACTTTA CCGGCCAGCT TCTGCTTCAC GCGGTGACAC TGATTTTCAA ACGTGAGGTT 
CTGGCAAACA AAAACAAATG GATGAGCACG CTTCGCTCCG CTTTGCCACT TCTGCTCCTT 
GGTTACCAGG TGCGACCGAT CGATGATTCG AGTACCAACC AATGGATAAA ACACTGCGAC 
GCGGAACAGA AGCAGTTGAT ACAGATGTGG CGCCCTCAGG GCGCCTTTCG ATGTGCCCAG 
CTTGGACGAA CCCTTTTGGG CTGCTTGGAC CGGTCACAAA TGGAAATTAA AAACGATAGA 
GAAAATGCCG AGTTTGATGA GAATAAGAAT TCGGGAAATT CTATGCCGGG ATTATTTGCC 
GACTCCGAAG AGCTGTTGGC CAGCGCTCAC CAGCAATGCT TGGACAAGAG CTGGCGAAGT 
CAAATTTACC AACAGCTCTT TACCCACGCC GAACACAAGC TAAAGGATAC GTCTTCGCAT 
CTGGTGCGGA ACCTGCGTCT GCAGCTGCCT CTTTTTGAGT GGCCGAATCT GGCGCATATC 
GAGAACTACG AACTGCAAGC GTTGGTGCTG CCGCCACACT CCTTAGCCCA ACATGTCTAC 
CTGGCTCTGG GCACCGACCC GAACAAACTT GGAGATGCGC CACGCGTGGT CTTTTATGAG 
GGTTTCCAAC GGGATGTTAA GCAGAATCTA AACTACTGTG GGCAAGATTC AATCAGCCAG 
GTAGATGTGG ACCTCTACCT TTATGCCACA ACCATTCAGA CCAGACGTAA ATTGCAAATC 
CAGCGTGAGG TGTATGACTC CTCCAATTTG GGAAACCGCA ATGCTGCCGC CCGGCCACAT 
ATGATGCCAT TTGCCAATCT AGTGGGTCAA CTAGGCGCAC CGGAGCAAAG TAACTGGTGG 
GATCTGGTGG TGCGACTTAA CTCGAACCAG CTTATCACTG AAGGCAATCG CGCCGAGCAA 
CGAGCTCAAC TACAACATGG ATTGGAAGCG GTGCGCGGCG TTAATGGCCC AAAAGCAGAT 
GCCATCATCA TTTTTCAATT GGGAAAGATT TTAAATTCCC GATCGGATCG TTCTTCTCTA 
GAAGCGCGTA TCGATACCCT GTACAGGCAA GGATTTTCAA TTTTACGCCA CCAACACAAC 
CAGCAGATGG AATCCTATGT CCGCGTCTTT AAGTACGGAT CCGCCGGCTC AACGGCAGCA 
TGGCAAGATC TTCAGTCGTT GGCTGAGGAA GCCGTCACCT ATTTTAGCGA GAAAATGTTT 
AGGATTGGAC AGTATGAGCA GTTTTTGGAC GAGGTCCGGG GACTTCATCT GCCCATGGCC 
TACTTTTTGC AATCGGAGGC GTGTCATCAT CTTGAGGAAT CTTCCAAGCT GCCTCGAACT 
TCCCGAGATC GTTACTCAGA GCGAAGACGT GAATGCCTGC AGAAAACGCA AAAGTTAATC 
AAAAATGATG ATAAGCACCC ACTCATAGCG GCCATGCATA GACATCAACA GGACCGCAAT 
AGTCGGGGTA TCGATAATAG CTTCGGCAGT CCGGATGTTC ATAACAATTC ATCCGCCTAC 
GAGGACGCAG AAGATGATTT CTATTCCCAT GCAGCCTTCT CTGCCAATCG ATCACGTCGT 
CAGTTAGAAG TCACCCCAGT CACTCCCATT GTTATGGCAC AACCGAGTCA GGAAATGGAG 
CAGGCTGTCA AGCAGATCAG CAAGTCACTG TGTGTGCTCA AGGACGATGT GAGTGTGGGA 
ATGGAAGCCA TGCGGCAGGA TATCAAAGTT CTTACTGAGA AGTTCACTGG CCTGGAGGAT 
CTGCTTAAGA AGATCAAGAT CAGCAGTCGA GACACGCCTA CACGAGATGT CGATCCCGCA 
GCTGCCTTAG GCCTGGACGA CCTCTTCATA ATTGAGGATG CACTTGCGGA GCATCAGCAG 
CAGCAACAGC ATCAGCAGCA GCAGAGCCAC AACCAAGGTG CAATACACCC AGTCGTACCA 
AATCCATATA CTTCTGGCTT CTACAACGGG ATGCCAAACA CGCCATCCGC CCAAGAGCGC 
TTTCTGCAGG GACCATACGG GAGTCCCATG TTTAACCAAA ACCAGATGTA CAACTACTAC 
GCTGCCCAAG CTCAGGCTCA AGCGCAAGCG CAGTTCTTGC GGACTCCTCC AGCTCCAGGA 
TCAATTCCAC CTCCGAATAT GTTTGGTCCT CGAAATCCAA ACTTTGGCCT GCCCAGTATG 
TTTCCCCCGC CGACCGTGCC TTCGGTAGCA CCTTACATTG ACGCCATGGG CAACTTTACC 
CAGCCTCCCC CAAGTTTGAT ACCTCCTCCC GCTCAGCCAG CAGCTCCTCC GGCTCCCTTG 
AATATTCTTG AGTCCAAGCC TGTGGTTGCA CTGCCAACTC CAGGATTCTT CAATACGACA 
ACACCGGTCT TTGGAGCTTC GCCCATTCAA GTTCCGCAGT CGAAACCATT AACTGTTCCA 
ACGGTGCCAA TACCTAGTAC TGCTCCTGCT CCACCAATTG CTGGAACTGT GAATCCACCG 
GCAACAACTG CTGTTCCGCC TCCTGTTCAT ATACCTCAAG TGGCTCCTTC TGTTCCTGCA 
CAGCCACCCG CACCCGCCCC TGTTAGTGTG CCGTCTATGT TTAACCGTGC GTTGAACAAT 
CAGCCCGTGG AAAAGGAGCC GCCAGCCAAT GTGGTCATAA CCAGTTCAGA TCCGTTGCCG 
AAACCCACGA CTGCCAGTGT CCAACCCACT TTAAGTGTGA CCATTCCAGC ACAGCATATC 
AAGCCAAGTT TGGTTCAAGC ACCGGAACAG CCGGCACAAT CGGCACAACC GGCACAGCCA 
TCGGTGTCAG GAGTAGGATC ACTCAGCTTT AACTTTGGCA GTAAGTCTTC CGAAAGTCCT 
TTTTCATTCA AGACACAGGT GGCTAAGGCA GCAGCCGAAA AACAAAAGGA ACAAGAGGAA 
GCTGAGCAAA ACCAAAGTGG GGCTACTGAT CCCAACAAAA CTCTGCCACA AGATACTTCA 
GCAGATGATT ACGATCCTCG TCCCGATTTC AAGCCCATCA TTCCACTACC CGATGAAGTG 
GAGGTTCGAA CAGGGGAGGA GGGCGAGGAT ATCAAGTTTA CCAGCCGAGC CAAGCTCTTT 
CGATATGTGG ACAAGGAGTG GAAGGAGCGC GGGACAGGGG TGATCAAAAT TTTATGCGAC 
AAAGCCACCG GCGTTTCGCG AGTCTTGATG CGTCGCGATC AAACTCACAA AGTGTGTGCT 
AATCACACAA TCACTGCAGA CATTACGATA AATGTTGCCA ACCAGGACAA GGATAAGAAA 
TCCCTTCTGT GGGCGGCAAA TGATTTTGCG GATGAGCAAG TTACATTGGA GAGATTCCTG 
GTGCGTTTCA AAACAGGGGA GTTGGCTGAA GAGTTCAGGG TGGCTTTTAC AAAGGCAAGC 
GAGGCCGCCA AGTCGAAGGA AACCGTGAAA CCGACGGTAA ACACAGCGGA GAAAGGATCA 
ACTGCGACTG CTCCAGCGGC ATTCAAGAGC TTTGTAACCA GCACCCCAGC AGCCAATTCG 
CTAATCAATA AGCCTCAGGA GCAGACAAAA ACTCAGCCTA ATCCGGACCC ACCAGCAACA 
GCAGCTAAGT CACTGTTTGG AACCCTGTCA GTCAGTGCTG CTCCTGCAAC ATCTGCGCCA 
GCCTCTGCCA CACCATTTGC CAGCTTTAGC TTCACGCCGA ATGGGTCATC AGGATTTGGA 
ACTTCCACCG CCTCTCCGTT CGGAAACCTT TCTTTTGGTA CCGCTTCTGC GGTGGGCAGC 
GGCAACAACA CTACTTTATT CACCACGGCT TTGATCAAGG ACAATACAGT TCAAGGCAAA 
ACGCTTCAGC AGGAGTCACA ATTAAACAAG TCAAACTCCT CGGACGCTGA GGAGGAGTAT 
GTGCCCACCG CTCAGTTCGT GCCTGTTATT GCCCTGCCCG ATATAGTGGA GGTGGTCACC 
GGCGAAGAGA ATGAGGATGT GCTCTTCGAG CACCGTGCCA AGCTTCTGCG TTGGGATAAG 
GAAGCGAATG AGTGGAAGGA GCGCGGCCTT GGTAATATGA AGCTCCTTCG GGATCGCACT 
GATCCCAACA AGGTTCGCCT GCTTATGCGG CGCGAGCAGG TCCATAAGCT GTGCTGCAAT 
CAGCGACTGT TGCCTGAGAC CAAGTTCACT TATGCAACGA ACTGTAAAGC GGCCGTCACA 
TGGGGTGCTC AGGATTATTC CGATGAGGAG TTAACCACCG CATTGCTCGC TGTGCGATTT 
AAGTCGCAAG ACATCTGTCA GCAATTCCTC GAGGCCGTGC AAAAGGCTCA ACAAAGTATT 
GGAAATGAAC CCAAAAAGGA GGAGGTCCCT TCTGCCGCTG GAGAAAAGGA GAAGCCAATT 
AAAGGCTTTG GAGATGCCTT TAAGCCTAAG GCAGGCAGTT GGAACTGCCA AGCCTGCTAT 
ACCAACAATG GCCAGGATCA GCTCTACTGC TTGGCCTGCC AGGAGCCCAA AGATGCCACT 
GTTCCCCCCA AGCAATCGGG GTTAGACCAA GGGAACGCGT TAAATCTTAC CACTAGCTCC 
TCAAACAAGT TTTCGTTTGG CTTTGCCTCC TCAGCGACTC TTCCAGCCAC AGGTGGATTT 
AGTTTCGGTG GCGCCACACA GCCAAAGGAG AAGCCAGCAG TGGCAGTTGT TACCGCCTCG 
GCTTCGGCAC CTACGTCAGT TCAAACGGCA GCCCTTGGAT TTGGAAAGTC TTCAATGACT 
TCTGGATTCG GGGACGCCTT CAAACCTGCA GTCGGTAGTT GGTCATGTAG CGCATGCTAT 
GTGAACAATC CCGGAGAGTC TCTTTACTGC AGCGCCTGTG ATGCACCCAA GAATGACACG 
GTACCACAGA AGGAGAAGTC ACTAGGATCG GGACTTAATC TGCCACCTAC CTCCAAGTTT 
AGCTTTGGTT TCGGAGCCGC AGCGGCTGGT GACAAAGATC AGGCTGGCGA TGGAGCCACG 
TTCAACTTCG CTGCGATGCC CGCAGCTGTT GCTCCGACTA CCTCAATTGG ATCCAGCAGC 
TTCACCTTCT CCATGACCAA GCCCAAGCCC GACCAGCAGC AGCCAAATAG TACAGCAGCT 
AAGGAGGATG AAGATAACGA TTCGCAAGAG GTGGAGGAGG AGGAGAACAA CACTTACTTT 
TCACCAGTAA TCCCATTACC CGATAAGATT GATGTAAAAA CAGGTGAGGA GGACGAGGAG 
CTATTGTATG TGCATAAGGC CAAGTTGTAC CGCCTAAACG AGAGCGATTG GAAAGAGCGC 
GGCTTGGGGG ATGTGAAGAT CCTGCGCCAT AGGCAGACCA AAAAGCTGCG CGTGGTCATG 
CGTCGGGAGC AGGTGTTCAA GATCTGTCTT AACCATGTGC TTAACGAGAA TGTGGTGTAC 
AGGGAGAAGA CTGAAACGTC GTGGATGTTT GCTGTACACG ATTTTAGCGA GGGCGAGAGT 
GTCCTAGAGC GATTCACTTT GCGTTTTAAA AACAAGGAGG TGGCACAAGG ATTTATGGAA 
GCGATCAAAA ATGCTCTTAA TGAAACCGCA AAGCCGATAG AAGATAGCCC AGTGGTCGGC 
TCCGTTTCCC AATCGACGGA AGCAAATAAA CCGTCCCAAA AAAATGATGG GGCGGCAAAG 
AGCAGAGGAG GCGAATCGGA AGTACTTGAT GTTGGCAAAA CCTCTTCTGT AAGGCCAACC 
ACGCACGAAG TAATCCCTCC ACTTCCCATG ACCCTTCCCC TACTGACTCT CCCCCAGCCA 
TTGGCCAAGC CTAACGATTA CCAAACACCT GCCACCATAT TGTTCAAAGG CAGTTCGCTG 
AGCAGAAATA ACTCTTCGGC TTCTGAGGCA AGCAAAACGC CTAGTTCAGC ATTCATTTTC 
GGTAGCACGG ATAAATCGGA GCCTGGAAAG GATGCTGGCC CATTAGCTAA TCTTCAAAAA 
CTAGCATCAG GCGAAGGACA AGGAAACGTT TTGGGATCTA TATTCCGTAG CGGCTCGTCA 
AACGAAAATT CGTCTGACGG TTCTGTCAAG TTCTTTTTCG GGGGTGGAAA TAAAGCGGCT 
GAGCAACAGA AGAAAGATTC CTCAGAGTCT GTCTTCGGAG GCAATAAAGC GGATTCCCAA 
TCGCCAGCGA CCCAAGAAGC ACCGAAGTTG GCCTTCGGTG GAATTGCCGC ACCTGTGTTC 
GGAGACGCCA ATCCTTTTGG AGGCCACAAA GTTAATTTGC AAAAATCAGA TGGCAAGGAA 
GAACCAAAAT CTATCATTGG CGGAACCCCA TTGTTATTTG GAGGGTCCAA CGCCTTTGGA 
ATACCCAAAA TTGAAACCCA GTCACCTGCT AAAGACTTTG TGTTTGGCAG TGCACCCGCT 
TTTGGACAAA TGGCAACCTT CTCATTTACA GCTGCAAAAA ATGAAAAGGA AAAGGACATA 
ACCTCGAATA ACACCACTGA TCTTAAAGCC GAAGGCAAGG AAAAGAAGGA GTTGGTGCCG 
GAGACGACCA GCACATTTGC CGATTTGGCC AAGACAGGCA GCACGTTTGC AGATTTGGCA 
AGCAATCCGG GCGGCACTTT TGCGGACTTA GCGAACAAGA CGGGCAACGA CTTTGCCAAC 
CTGTCGGCCA ATAGCCAGGG CACTACCGTG GGATTCAACA AGTCCGCCGG GGGCGGCTTT 
TATAACCTCA CGCATCAGAA CGCTTTCAAG AACTTCGAGT CGCCGCAAGC CACAGAAGAG 
TGCGATGATG ACGGCGACGC GACCACTGAT GACAACTACG ATCCGCACTA TGATGCCATT 
GTAGAGCTGC CGGATGAGAT AGTTGTCACC ACGGGAGAGG AGAACGAGAC TAAGCTGTTT 
GGCGAGCGGG CGAAGCTTTA TCGCTATGAC GCCGAATCGA AACAATGGAA GGAGCGAGGT 
GTCGGTGAGA TAAAGGTGCT GGAGCACCCG GAGCTGCAGA CATTCCGACT GATCATGCGG 
CAGGAGCAGA TCCACAAGCT GGTGCTTAAC ATGAATATCT CCGCCTCCCT GCAAATGGAT 
TACATGAACG CGCAGATGAA GAGCTTCCTG TGGGCCGGCT ACAACTACGC GGTGGACGCA 
GAGGGTAAAG TTGACACCGA GGGCGTCCTG GAACGCCTTG CCTGTCGATT CGCCAAGGAA 
GAGATCGCCA GTGAGTTCCT CAACACGGTC AATTCGTGCA TAAAACGAGC CAAGGCCTTG 
CAAGGTGATG AGGAGAACAA GAATGACGAC GCGCCGGAGG AGCAGGCGAG CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247302.1
CDS	284..8440
Translation	MFTTRKEVDAHVHKMLGKLQPGRERDIKGLAVARLYMKVQEYPKAIEYLNGYLRVRDDAVGHNMIATCYSRLNPPDVTEALQHYQRSIQIDPRQSEVVIDACELLVKENNASITECARYWLDQANSLDLSGNKQVFNLRMRVNLADSNGERDDTSGGDGEQNTLEILMYKELQARPQDVNIRIQLLRSYVEKMKIDQAFNYALKTELESKNCTSQSNEWYEQIWMVLFKIEMAKDVKKNWRFWHFALHTLDRLVQLSLEGSGLADSSKQLFRLDQYLFKFSTSIERSGDAPQRDLHQACIDHFTGQLLLHAVTLIFKREVLANKNKWMSTLRSALPLLLLGYQVRPIDDSSTNQWIKHCDAEQKQLIQMWRPQGAFRCAQLGRTLLGCLDRSQMEIKNDRENAEFDENKNSGNSMPGLFADSEELLASAHQQCLDKSWRSQIYQQLFTHAEHKLKDTSSHLVRNLRLQLPLFEWPNLAHIENYELQALVLPPHSLAQHVYLALGTDPNKLGDAPRVVFYEGFQRDVKQNLNYCGQDSISQVDVDLYLYATTIQTRRKLQIQREVYDSSNLGNRNAAARPHMMPFANLVGQLGAPEQSNWWDLVVRLNSNQLITEGNRAEQRAQLQHGLEAVRGVNGPKADAIIIFQLGKILNSRSDRSSLEARIDTLYRQGFSILRHQHNQQMESYVRVFKYGSAGSTAAWQDLQSLAEEAVTYFSEKMFRIGQYEQFLDEVRGLHLPMAYFLQSEACHHLEESSKLPRTSRDRYSERRRECLQKTQKLIKNDDKHPLIAAMHRHQQDRNSRGIDNSFGSPDVHNNSSAYEDAEDDFYSHAAFSANRSRRQLEVTPVTPIVMAQPSQEMEQAVKQISKSLCVLKDDVSVGMEAMRQDIKVLTEKFTGLEDLLKKIKISSRDTPTRDVDPAAALGLDDLFIIEDALAEHQQQQQHQQQQSHNQGAIHPVVPNPYTSGFYNGMPNTPSAQERFLQGPYGSPMFNQNQMYNYYAAQAQAQAQAQFLRTPPAPGSIPPPNMFGPRNPNFGLPSMFPPPTVPSVAPYIDAMGNFTQPPPSLIPPPAQPAAPPAPLNILESKPVVALPTPGFFNTTTPVFGASPIQVPQSKPLTVPTVPIPSTAPAPPIAGTVNPPATTAVPPPVHIPQVAPSVPAQPPAPAPVSVPSMFNRALNNQPVEKEPPANVVITSSDPLPKPTTASVQPTLSVTIPAQHIKPSLVQAPEQPAQSAQPAQPSVSGVGSLSFNFGSKSSESPFSFKTQVAKAAAEKQKEQEEAEQNQSGATDPNKTLPQDTSADDYDPRPDFKPIIPLPDEVEVRTGEEGEDIKFTSRAKLFRYVDKEWKERGTGVIKILCDKATGVSRVLMRRDQTHKVCANHTITADITINVANQDKDKKSLLWAANDFADEQVTLERFLVRFKTGELAEEFRVAFTKASEAAKSKETVKPTVNTAEKGSTATAPAAFKSFVTSTPAANSLINKPQEQTKTQPNPDPPATAAKSLFGTLSVSAAPATSAPASATPFASFSFTPNGSSGFGTSTASPFGNLSFGTASAVGSGNNTTLFTTALIKDNTVQGKTLQQESQLNKSNSSDAEEEYVPTAQFVPVIALPDIVEVVTGEENEDVLFEHRAKLLRWDKEANEWKERGLGNMKLLRDRTDPNKVRLLMRREQVHKLCCNQRLLPETKFTYATNCKAAVTWGAQDYSDEELTTALLAVRFKSQDICQQFLEAVQKAQQSIGNEPKKEEVPSAAGEKEKPIKGFGDAFKPKAGSWNCQACYTNNGQDQLYCLACQEPKDATVPPKQSGLDQGNALNLTTSSSNKFSFGFASSATLPATGGFSFGGATQPKEKPAVAVVTASASAPTSVQTAALGFGKSSMTSGFGDAFKPAVGSWSCSACYVNNPGESLYCSACDAPKNDTVPQKEKSLGSGLNLPPTSKFSFGFGAAAAGDKDQAGDGATFNFAAMPAAVAPTTSIGSSSFTFSMTKPKPDQQQPNSTAAKEDEDNDSQEVEEEENNTYFSPVIPLPDKIDVKTGEEDEELLYVHKAKLYRLNESDWKERGLGDVKILRHRQTKKLRVVMRREQVFKICLNHVLNENVVYREKTETSWMFAVHDFSEGESVLERFTLRFKNKEVAQGFMEAIKNALNETAKPIEDSPVVGSVSQSTEANKPSQKNDGAAKSRGGESEVLDVGKTSSVRPTTHEVIPPLPMTLPLLTLPQPLAKPNDYQTPATILFKGSSLSRNNSSASEASKTPSSAFIFGSTDKSEPGKDAGPLANLQKLASGEGQGNVLGSIFRSGSSNENSSDGSVKFFFGGGNKAAEQQKKDSSESVFGGNKADSQSPATQEAPKLAFGGIAAPVFGDANPFGGHKVNLQKSDGKEEPKSIIGGTPLLFGGSNAFGIPKIETQSPAKDFVFGSAPAFGQMATFSFTAAKNEKEKDITSNNTTDLKAEGKEKKELVPETTSTFADLAKTGSTFADLASNPGGTFADLANKTGNDFANLSANSQGTTVGFNKSAGGGFYNLTHQNAFKNFESPQATEECDDDGDATTDDNYDPHYDAIVELPDEIVVTTGEENETKLFGERAKLYRYDAESKQWKERGVGEIKVLEHPELQTFRLIMRQEQIHKLVLNMNISASLQMDYMNAQMKSFLWAGYNYAVDAEGKVDTEGVLERLACRFAKEEIASEFLNTVNSCIKRAKALQGDEENKNDDAPEEQASS

Target ORF information:

RefSeq Version	NM_001260373.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 358kD, transcript variant B (Nup358), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260373.2

ORF Insert Sequence:

ATGTTTACAA CGCGAAAAGA AGTGGACGCA CACGTCCACA AAATGTTGGG AAAACTTCAG 
CCGGGAAGAG AGCGCGATAT CAAAGGCTTG GCTGTAGCTC GATTGTACAT GAAAGTTCAG 
GAATACCCAA AAGCCATCGA GTATTTGAAC GGCTATCTCC GGGTGAGGGA CGACGCTGTA 
GGCCATAACA TGATTGCCAC TTGCTACAGT AGGCTGAACC CGCCCGATGT GACAGAGGCC 
CTGCAGCACT ACCAACGTTC TATTCAAATT GATCCGCGTC AGTCAGAGGT TGTCATTGAC 
GCCTGCGAAC TTCTGGTGAA GGAGAACAAC GCATCGATCA CGGAGTGCGC CAGGTACTGG 
TTGGATCAGG CTAATAGCCT TGATTTAAGC GGCAACAAGC AAGTCTTCAA CCTGCGAATG 
CGTGTAAATC TTGCGGACAG TAACGGCGAA AGAGATGACA CCAGTGGCGG AGACGGCGAG 
CAAAATACCC TCGAGATTCT TATGTACAAG GAGCTGCAGG CGCGTCCCCA GGATGTTAAT 
ATACGCATAC AACTGCTGCG CAGCTATGTT GAAAAAATGA AAATAGACCA GGCCTTCAAC 
TATGCTCTAA AAACGGAACT GGAGTCGAAA AACTGCACGA GCCAGTCGAA CGAATGGTAC 
GAGCAGATCT GGATGGTCCT TTTCAAGATA GAAATGGCCA AGGATGTAAA GAAGAACTGG 
CGATTTTGGC ACTTCGCCTT GCACACTCTC GATCGTCTCG TGCAGCTAAG CCTGGAGGGC 
AGTGGACTGG CAGACAGCAG CAAGCAGTTG TTCAGACTTG ATCAATATCT ATTCAAGTTT 
AGCACTTCGA TAGAGAGGTC AGGGGACGCT CCGCAGCGGG ATCTCCACCA GGCTTGTATT 
GACCACTTTA CCGGCCAGCT TCTGCTTCAC GCGGTGACAC TGATTTTCAA ACGTGAGGTT 
CTGGCAAACA AAAACAAATG GATGAGCACG CTTCGCTCCG CTTTGCCACT TCTGCTCCTT 
GGTTACCAGG TGCGACCGAT CGATGATTCG AGTACCAACC AATGGATAAA ACACTGCGAC 
GCGGAACAGA AGCAGTTGAT ACAGATGTGG CGCCCTCAGG GCGCCTTTCG ATGTGCCCAG 
CTTGGACGAA CCCTTTTGGG CTGCTTGGAC CGGTCACAAA TGGAAATTAA AAACGATAGA 
GAAAATGCCG AGTTTGATGA GAATAAGAAT TCGGGAAATT CTATGCCGGG ATTATTTGCC 
GACTCCGAAG AGCTGTTGGC CAGCGCTCAC CAGCAATGCT TGGACAAGAG CTGGCGAAGT 
CAAATTTACC AACAGCTCTT TACCCACGCC GAACACAAGC TAAAGGATAC GTCTTCGCAT 
CTGGTGCGGA ACCTGCGTCT GCAGCTGCCT CTTTTTGAGT GGCCGAATCT GGCGCATATC 
GAGAACTACG AACTGCAAGC GTTGGTGCTG CCGCCACACT CCTTAGCCCA ACATGTCTAC 
CTGGCTCTGG GCACCGACCC GAACAAACTT GGAGATGCGC CACGCGTGGT CTTTTATGAG 
GGTTTCCAAC GGGATGTTAA GCAGAATCTA AACTACTGTG GGCAAGATTC AATCAGCCAG 
GTAGATGTGG ACCTCTACCT TTATGCCACA ACCATTCAGA CCAGACGTAA ATTGCAAATC 
CAGCGTGAGG TGTATGACTC CTCCAATTTG GGAAACCGCA ATGCTGCCGC CCGGCCACAT 
ATGATGCCAT TTGCCAATCT AGTGGGTCAA CTAGGCGCAC CGGAGCAAAG TAACTGGTGG 
GATCTGGTGG TGCGACTTAA CTCGAACCAG CTTATCACTG AAGGCAATCG CGCCGAGCAA 
CGAGCTCAAC TACAACATGG ATTGGAAGCG GTGCGCGGCG TTAATGGCCC AAAAGCAGAT 
GCCATCATCA TTTTTCAATT GGGAAAGATT TTAAATTCCC GATCGGATCG TTCTTCTCTA 
GAAGCGCGTA TCGATACCCT GTACAGGCAA GGATTTTCAA TTTTACGCCA CCAACACAAC 
CAGCAGATGG AATCCTATGT CCGCGTCTTT AAGTACGGAT CCGCCGGCTC AACGGCAGCA 
TGGCAAGATC TTCAGTCGTT GGCTGAGGAA GCCGTCACCT ATTTTAGCGA GAAAATGTTT 
AGGATTGGAC AGTATGAGCA GTTTTTGGAC GAGGTCCGGG GACTTCATCT GCCCATGGCC 
TACTTTTTGC AATCGGAGGC GTGTCATCAT CTTGAGGAAT CTTCCAAGCT GCCTCGAACT 
TCCCGAGATC GTTACTCAGA GCGAAGACGT GAATGCCTGC AGAAAACGCA AAAGTTAATC 
AAAAATGATG ATAAGCACCC ACTCATAGCG GCCATGCATA GACATCAACA GGACCGCAAT 
AGTCGGGGTA TCGATAATAG CTTCGGCAGT CCGGATGTTC ATAACAATTC ATCCGCCTAC 
GAGGACGCAG AAGATGATTT CTATTCCCAT GCAGCCTTCT CTGCCAATCG ATCACGTCGT 
CAGTTAGAAG TCACCCCAGT CACTCCCATT GTTATGGCAC AACCGAGTCA GGAAATGGAG 
CAGGCTGTCA AGCAGATCAG CAAGTCACTG TGTGTGCTCA AGGACGATGT GAGTGTGGGA 
ATGGAAGCCA TGCGGCAGGA TATCAAAGTT CTTACTGAGA AGTTCACTGG CCTGGAGGAT 
CTGCTTAAGA AGATCAAGAT CAGCAGTCGA GACACGCCTA CACGAGATGT CGATCCCGCA 
GCTGCCTTAG GCCTGGACGA CCTCTTCATA ATTGAGGATG CACTTGCGGA GCATCAGCAG 
CAGCAACAGC ATCAGCAGCA GCAGAGCCAC AACCAAGGTG CAATACACCC AGTCGTACCA 
AATCCATATA CTTCTGGCTT CTACAACGGG ATGCCAAACA CGCCATCCGC CCAAGAGCGC 
TTTCTGCAGG GACCATACGG GAGTCCCATG TTTAACCAAA ACCAGATGTA CAACTACTAC 
GCTGCCCAAG CTCAGGCTCA AGCGCAAGCG CAGTTCTTGC GGACTCCTCC AGCTCCAGGA 
TCAATTCCAC CTCCGAATAT GTTTGGTCCT CGAAATCCAA ACTTTGGCCT GCCCAGTATG 
TTTCCCCCGC CGACCGTGCC TTCGGTAGCA CCTTACATTG ACGCCATGGG CAACTTTACC 
CAGCCTCCCC CAAGTTTGAT ACCTCCTCCC GCTCAGCCAG CAGCTCCTCC GGCTCCCTTG 
AATATTCTTG AGTCCAAGCC TGTGGTTGCA CTGCCAACTC CAGGATTCTT CAATACGACA 
ACACCGGTCT TTGGAGCTTC GCCCATTCAA GTTCCGCAGT CGAAACCATT AACTGTTCCA 
ACGGTGCCAA TACCTAGTAC TGCTCCTGCT CCACCAATTG CTGGAACTGT GAATCCACCG 
GCAACAACTG CTGTTCCGCC TCCTGTTCAT ATACCTCAAG TGGCTCCTTC TGTTCCTGCA 
CAGCCACCCG CACCCGCCCC TGTTAGTGTG CCGTCTATGT TTAACCGTGC GTTGAACAAT 
CAGCCCGTGG AAAAGGAGCC GCCAGCCAAT GTGGTCATAA CCAGTTCAGA TCCGTTGCCG 
AAACCCACGA CTGCCAGTGT CCAACCCACT TTAAGTGTGA CCATTCCAGC ACAGCATATC 
AAGCCAAGTT TGGTTCAAGC ACCGGAACAG CCGGCACAAT CGGCACAACC GGCACAGCCA 
TCGGTGTCAG GAGTAGGATC ACTCAGCTTT AACTTTGGCA GTAAGTCTTC CGAAAGTCCT 
TTTTCATTCA AGACACAGGT GGCTAAGGCA GCAGCCGAAA AACAAAAGGA ACAAGAGGAA 
GCTGAGCAAA ACCAAAGTGG GGCTACTGAT CCCAACAAAA CTCTGCCACA AGATACTTCA 
GCAGATGATT ACGATCCTCG TCCCGATTTC AAGCCCATCA TTCCACTACC CGATGAAGTG 
GAGGTTCGAA CAGGGGAGGA GGGCGAGGAT ATCAAGTTTA CCAGCCGAGC CAAGCTCTTT 
CGATATGTGG ACAAGGAGTG GAAGGAGCGC GGGACAGGGG TGATCAAAAT TTTATGCGAC 
AAAGCCACCG GCGTTTCGCG AGTCTTGATG CGTCGCGATC AAACTCACAA AGTGTGTGCT 
AATCACACAA TCACTGCAGA CATTACGATA AATGTTGCCA ACCAGGACAA GGATAAGAAA 
TCCCTTCTGT GGGCGGCAAA TGATTTTGCG GATGAGCAAG TTACATTGGA GAGATTCCTG 
GTGCGTTTCA AAACAGGGGA GTTGGCTGAA GAGTTCAGGG TGGCTTTTAC AAAGGCAAGC 
GAGGCCGCCA AGTCGAAGGA AACCGTGAAA CCGACGGTAA ACACAGCGGA GAAAGGATCA 
ACTGCGACTG CTCCAGCGGC ATTCAAGAGC TTTGTAACCA GCACCCCAGC AGCCAATTCG 
CTAATCAATA AGCCTCAGGA GCAGACAAAA ACTCAGCCTA ATCCGGACCC ACCAGCAACA 
GCAGCTAAGT CACTGTTTGG AACCCTGTCA GTCAGTGCTG CTCCTGCAAC ATCTGCGCCA 
GCCTCTGCCA CACCATTTGC CAGCTTTAGC TTCACGCCGA ATGGGTCATC AGGATTTGGA 
ACTTCCACCG CCTCTCCGTT CGGAAACCTT TCTTTTGGTA CCGCTTCTGC GGTGGGCAGC 
GGCAACAACA CTACTTTATT CACCACGGCT TTGATCAAGG ACAATACAGT TCAAGGCAAA 
ACGCTTCAGC AGGAGTCACA ATTAAACAAG TCAAACTCCT CGGACGCTGA GGAGGAGTAT 
GTGCCCACCG CTCAGTTCGT GCCTGTTATT GCCCTGCCCG ATATAGTGGA GGTGGTCACC 
GGCGAAGAGA ATGAGGATGT GCTCTTCGAG CACCGTGCCA AGCTTCTGCG TTGGGATAAG 
GAAGCGAATG AGTGGAAGGA GCGCGGCCTT GGTAATATGA AGCTCCTTCG GGATCGCACT 
GATCCCAACA AGGTTCGCCT GCTTATGCGG CGCGAGCAGG TCCATAAGCT GTGCTGCAAT 
CAGCGACTGT TGCCTGAGAC CAAGTTCACT TATGCAACGA ACTGTAAAGC GGCCGTCACA 
TGGGGTGCTC AGGATTATTC CGATGAGGAG TTAACCACCG CATTGCTCGC TGTGCGATTT 
AAGTCGCAAG ACATCTGTCA GCAATTCCTC GAGGCCGTGC AAAAGGCTCA ACAAAGTATT 
GGAAATGAAC CCAAAAAGGA GGAGGTCCCT TCTGCCGCTG GAGAAAAGGA GAAGCCAATT 
AAAGGCTTTG GAGATGCCTT TAAGCCTAAG GCAGGCAGTT GGAACTGCCA AGCCTGCTAT 
ACCAACAATG GCCAGGATCA GCTCTACTGC TTGGCCTGCC AGGAGCCCAA AGATGCCACT 
GTTCCCCCCA AGCAATCGGG GTTAGACCAA GGGAACGCGT TAAATCTTAC CACTAGCTCC 
TCAAACAAGT TTTCGTTTGG CTTTGCCTCC TCAGCGACTC TTCCAGCCAC AGGTGGATTT 
AGTTTCGGTG GCGCCACACA GCCAAAGGAG AAGCCAGCAG TGGCAGTTGT TACCGCCTCG 
GCTTCGGCAC CTACGTCAGT TCAAACGGCA GCCCTTGGAT TTGGAAAGTC TTCAATGACT 
TCTGGATTCG GGGACGCCTT CAAACCTGCA GTCGGTAGTT GGTCATGTAG CGCATGCTAT 
GTGAACAATC CCGGAGAGTC TCTTTACTGC AGCGCCTGTG ATGCACCCAA GAATGACACG 
GTACCACAGA AGGAGAAGTC ACTAGGATCG GGACTTAATC TGCCACCTAC CTCCAAGTTT 
AGCTTTGGTT TCGGAGCCGC AGCGGCTGGT GACAAAGATC AGGCTGGCGA TGGAGCCACG 
TTCAACTTCG CTGCGATGCC CGCAGCTGTT GCTCCGACTA CCTCAATTGG ATCCAGCAGC 
TTCACCTTCT CCATGACCAA GCCCAAGCCC GACCAGCAGC AGCCAAATAG TACAGCAGCT 
AAGGAGGATG AAGATAACGA TTCGCAAGAG GTGGAGGAGG AGGAGAACAA CACTTACTTT 
TCACCAGTAA TCCCATTACC CGATAAGATT GATGTAAAAA CAGGTGAGGA GGACGAGGAG 
CTATTGTATG TGCATAAGGC CAAGTTGTAC CGCCTAAACG AGAGCGATTG GAAAGAGCGC 
GGCTTGGGGG ATGTGAAGAT CCTGCGCCAT AGGCAGACCA AAAAGCTGCG CGTGGTCATG 
CGTCGGGAGC AGGTGTTCAA GATCTGTCTT AACCATGTGC TTAACGAGAA TGTGGTGTAC 
AGGGAGAAGA CTGAAACGTC GTGGATGTTT GCTGTACACG ATTTTAGCGA GGGCGAGAGT 
GTCCTAGAGC GATTCACTTT GCGTTTTAAA AACAAGGAGG TGGCACAAGG ATTTATGGAA 
GCGATCAAAA ATGCTCTTAA TGAAACCGCA AAGCCGATAG AAGATAGCCC AGTGGTCGGC 
TCCGTTTCCC AATCGACGGA AGCAAATAAA CCGTCCCAAA AAAATGATGG GGCGGCAAAG 
AGCAGAGGAG GCGAATCGGA AGTACTTGAT GTTGGCAAAA CCTCTTCTGT AAGGCCAACC 
ACGCACGAAG TAATCCCTCC ACTTCCCATG ACCCTTCCCC TACTGACTCT CCCCCAGCCA 
TTGGCCAAGC CTAACGATTA CCAAACACCT GCCACCATAT TGTTCAAAGG CAGTTCGCTG 
AGCAGAAATA ACTCTTCGGC TTCTGAGGCA AGCAAAACGC CTAGTTCAGC ATTCATTTTC 
GGTAGCACGG ATAAATCGGA GCCTGGAAAG GATGCTGGCC CATTAGCTAA TCTTCAAAAA 
CTAGCATCAG GCGAAGGACA AGGAAACGTT TTGGGATCTA TATTCCGTAG CGGCTCGTCA 
AACGAAAATT CGTCTGACGG TTCTGTCAAG TTCTTTTTCG GGGGTGGAAA TAAAGCGGCT 
GAGCAACAGA AGAAAGATTC CTCAGAGTCT GTCTTCGGAG GCAATAAAGC GGATTCCCAA 
TCGCCAGCGA CCCAAGAAGC ACCGAAGTTG GCCTTCGGTG GAATTGCCGC ACCTGTGTTC 
GGAGACGCCA ATCCTTTTGG AGGCCACAAA GTTAATTTGC AAAAATCAGA TGGCAAGGAA 
GAACCAAAAT CTATCATTGG CGGAACCCCA TTGTTATTTG GAGGGTCCAA CGCCTTTGGA 
ATACCCAAAA TTGAAACCCA GTCACCTGCT AAAGACTTTG TGTTTGGCAG TGCACCCGCT 
TTTGGACAAA TGGCAACCTT CTCATTTACA GCTGCAAAAA ATGAAAAGGA AAAGGACATA 
ACCTCGAATA ACACCACTGA TCTTAAAGCC GAAGGCAAGG AAAAGAAGGA GTTGGTGCCG 
GAGACGACCA GCACATTTGC CGATTTGGCC AAGACAGGCA GCACGTTTGC AGATTTGGCA 
AGCAATCCGG GCGGCACTTT TGCGGACTTA GCGAACAAGA CGGGCAACGA CTTTGCCAAC 
CTGTCGGCCA ATAGCCAGGG CACTACCGTG GGATTCAACA AGTCCGCCGG GGGCGGCTTT 
TATAACCTCA CGCATCAGAA CGCTTTCAAG AACTTCGAGT CGCCGCAAGC CACAGAAGAG 
TGCGATGATG ACGGCGACGC GACCACTGAT GACAACTACG ATCCGCACTA TGATGCCATT 
GTAGAGCTGC CGGATGAGAT AGTTGTCACC ACGGGAGAGG AGAACGAGAC TAAGCTGTTT 
GGCGAGCGGG CGAAGCTTTA TCGCTATGAC GCCGAATCGA AACAATGGAA GGAGCGAGGT 
GTCGGTGAGA TAAAGGTGCT GGAGCACCCG GAGCTGCAGA CATTCCGACT GATCATGCGG 
CAGGAGCAGA TCCACAAGCT GGTGCTTAAC ATGAATATCT CCGCCTCCCT GCAAATGGAT 
TACATGAACG CGCAGATGAA GAGCTTCCTG TGGGCCGGCT ACAACTACGC GGTGGACGCA 
GAGGGTAAAG TTGACACCGA GGGCGTCCTG GAACGCCTTG CCTGTCGATT CGCCAAGGAA 
GAGATCGCCA GTGAGTTCCT CAACACGGTC AATTCGTGCA TAAAACGAGC CAAGGCCTTG 
CAAGGTGATG AGGAGAACAA GAATGACGAC GCGCCGGAGG AGCAGGCGAG CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup358 ( NM_143104.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651361.2 Drosophila melanogaster nucleoporin 358kD, transcript variant A (Nup358), mRNA.

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CloneID	OFa08829
Clone ID Related Accession (Same CDS sequence)	NM_143104.3
Accession Version	NM_143104.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 358kD, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143104.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTACAA CGCGAAAAGA AGTGGACGCA CACGTCCACA AAATGTTGGG AAAACTTCAG 
CCGGGAAGAG AGCGCGATAT CAAAGGCTTG GCTGTAGCTC GATTGTACAT GAAAGTTCAG 
GAATACCCAA AAGCCATCGA GTATTTGAAC GGCTATCTCC GGGTGAGGGA CGACGCTGTA 
GGCCATAACA TGATTGCCAC TTGCTACAGT AGGCTGAACC CGCCCGATGT GACAGAGGCC 
CTGCAGCACT ACCAACGTTC TATTCAAATT GATCCGCGTC AGTCAGAGGT TGTCATTGAC 
GCCTGCGAAC TTCTGGTGAA GGAGAACAAC GCATCGATCA CGGAGTGCGC CAGGTACTGG 
TTGGATCAGG CTAATAGCCT TGATTTAAGC GGCAACAAGC AAGTCTTCAA CCTGCGAATG 
CGTGTAAATC TTGCGGACAG TAACGGCGAA AGAGATGACA CCAGTGGCGG AGACGGCGAG 
CAAAATACCC TCGAGATTCT TATGTACAAG GAGCTGCAGG CGCGTCCCCA GGATGTTAAT 
ATACGCATAC AACTGCTGCG CAGCTATGTT GAAAAAATGA AAATAGACCA GGCCTTCAAC 
TATGCTCTAA AAACGGAACT GGAGTCGAAA AACTGCACGA GCCAGTCGAA CGAATGGTAC 
GAGCAGATCT GGATGGTCCT TTTCAAGATA GAAATGGCCA AGGATGTAAA GAAGAACTGG 
CGATTTTGGC ACTTCGCCTT GCACACTCTC GATCGTCTCG TGCAGCTAAG CCTGGAGGGC 
AGTGGACTGG CAGACAGCAG CAAGCAGTTG TTCAGACTTG ATCAATATCT ATTCAAGTTT 
AGCACTTCGA TAGAGAGGTC AGGGGACGCT CCGCAGCGGG ATCTCCACCA GGCTTGTATT 
GACCACTTTA CCGGCCAGCT TCTGCTTCAC GCGGTGACAC TGATTTTCAA ACGTGAGGTT 
CTGGCAAACA AAAACAAATG GATGAGCACG CTTCGCTCCG CTTTGCCACT TCTGCTCCTT 
GGTTACCAGG TGCGACCGAT CGATGATTCG AGTACCAACC AATGGATAAA ACACTGCGAC 
GCGGAACAGA AGCAGTTGAT ACAGATGTGG CGCCCTCAGG GCGCCTTTCG ATGTGCCCAG 
CTTGGACGAA CCCTTTTGGG CTGCTTGGAC CGGTCACAAA TGGAAATTAA AAACGATAGA 
GAAAATGCCG AGTTTGATGA GAATAAGAAT TCGGGAAATT CTATGCCGGG ATTATTTGCC 
GACTCCGAAG AGCTGTTGGC CAGCGCTCAC CAGCAATGCT TGGACAAGAG CTGGCGAAGT 
CAAATTTACC AACAGCTCTT TACCCACGCC GAACACAAGC TAAAGGATAC GTCTTCGCAT 
CTGGTGCGGA ACCTGCGTCT GCAGCTGCCT CTTTTTGAGT GGCCGAATCT GGCGCATATC 
GAGAACTACG AACTGCAAGC GTTGGTGCTG CCGCCACACT CCTTAGCCCA ACATGTCTAC 
CTGGCTCTGG GCACCGACCC GAACAAACTT GGAGATGCGC CACGCGTGGT CTTTTATGAG 
GGTTTCCAAC GGGATGTTAA GCAGAATCTA AACTACTGTG GGCAAGATTC AATCAGCCAG 
GTAGATGTGG ACCTCTACCT TTATGCCACA ACCATTCAGA CCAGACGTAA ATTGCAAATC 
CAGCGTGAGG TGTATGACTC CTCCAATTTG GGAAACCGCA ATGCTGCCGC CCGGCCACAT 
ATGATGCCAT TTGCCAATCT AGTGGGTCAA CTAGGCGCAC CGGAGCAAAG TAACTGGTGG 
GATCTGGTGG TGCGACTTAA CTCGAACCAG CTTATCACTG AAGGCAATCG CGCCGAGCAA 
CGAGCTCAAC TACAACATGG ATTGGAAGCG GTGCGCGGCG TTAATGGCCC AAAAGCAGAT 
GCCATCATCA TTTTTCAATT GGGAAAGATT TTAAATTCCC GATCGGATCG TTCTTCTCTA 
GAAGCGCGTA TCGATACCCT GTACAGGCAA GGATTTTCAA TTTTACGCCA CCAACACAAC 
CAGCAGATGG AATCCTATGT CCGCGTCTTT AAGTACGGAT CCGCCGGCTC AACGGCAGCA 
TGGCAAGATC TTCAGTCGTT GGCTGAGGAA GCCGTCACCT ATTTTAGCGA GAAAATGTTT 
AGGATTGGAC AGTATGAGCA GTTTTTGGAC GAGGTCCGGG GACTTCATCT GCCCATGGCC 
TACTTTTTGC AATCGGAGGC GTGTCATCAT CTTGAGGAAT CTTCCAAGCT GCCTCGAACT 
TCCCGAGATC GTTACTCAGA GCGAAGACGT GAATGCCTGC AGAAAACGCA AAAGTTAATC 
AAAAATGATG ATAAGCACCC ACTCATAGCG GCCATGCATA GACATCAACA GGACCGCAAT 
AGTCGGGGTA TCGATAATAG CTTCGGCAGT CCGGATGTTC ATAACAATTC ATCCGCCTAC 
GAGGACGCAG AAGATGATTT CTATTCCCAT GCAGCCTTCT CTGCCAATCG ATCACGTCGT 
CAGTTAGAAG TCACCCCAGT CACTCCCATT GTTATGGCAC AACCGAGTCA GGAAATGGAG 
CAGGCTGTCA AGCAGATCAG CAAGTCACTG TGTGTGCTCA AGGACGATGT GAGTGTGGGA 
ATGGAAGCCA TGCGGCAGGA TATCAAAGTT CTTACTGAGA AGTTCACTGG CCTGGAGGAT 
CTGCTTAAGA AGATCAAGAT CAGCAGTCGA GACACGCCTA CACGAGATGT CGATCCCGCA 
GCTGCCTTAG GCCTGGACGA CCTCTTCATA ATTGAGGATG CACTTGCGGA GCATCAGCAG 
CAGCAACAGC ATCAGCAGCA GCAGAGCCAC AACCAAGGTG CAATACACCC AGTCGTACCA 
AATCCATATA CTTCTGGCTT CTACAACGGG ATGCCAAACA CGCCATCCGC CCAAGAGCGC 
TTTCTGCAGG GACCATACGG GAGTCCCATG TTTAACCAAA ACCAGATGTA CAACTACTAC 
GCTGCCCAAG CTCAGGCTCA AGCGCAAGCG CAGTTCTTGC GGACTCCTCC AGCTCCAGGA 
TCAATTCCAC CTCCGAATAT GTTTGGTCCT CGAAATCCAA ACTTTGGCCT GCCCAGTATG 
TTTCCCCCGC CGACCGTGCC TTCGGTAGCA CCTTACATTG ACGCCATGGG CAACTTTACC 
CAGCCTCCCC CAAGTTTGAT ACCTCCTCCC GCTCAGCCAG CAGCTCCTCC GGCTCCCTTG 
AATATTCTTG AGTCCAAGCC TGTGGTTGCA CTGCCAACTC CAGGATTCTT CAATACGACA 
ACACCGGTCT TTGGAGCTTC GCCCATTCAA GTTCCGCAGT CGAAACCATT AACTGTTCCA 
ACGGTGCCAA TACCTAGTAC TGCTCCTGCT CCACCAATTG CTGGAACTGT GAATCCACCG 
GCAACAACTG CTGTTCCGCC TCCTGTTCAT ATACCTCAAG TGGCTCCTTC TGTTCCTGCA 
CAGCCACCCG CACCCGCCCC TGTTAGTGTG CCGTCTATGT TTAACCGTGC GTTGAACAAT 
CAGCCCGTGG AAAAGGAGCC GCCAGCCAAT GTGGTCATAA CCAGTTCAGA TCCGTTGCCG 
AAACCCACGA CTGCCAGTGT CCAACCCACT TTAAGTGTGA CCATTCCAGC ACAGCATATC 
AAGCCAAGTT TGGTTCAAGC ACCGGAACAG CCGGCACAAT CGGCACAACC GGCACAGCCA 
TCGGTGTCAG GAGTAGGATC ACTCAGCTTT AACTTTGGCA GTAAGTCTTC CGAAAGTCCT 
TTTTCATTCA AGACACAGGT GGCTAAGGCA GCAGCCGAAA AACAAAAGGA ACAAGAGGAA 
GCTGAGCAAA ACCAAAGTGG GGCTACTGAT CCCAACAAAA CTCTGCCACA AGATACTTCA 
GCAGATGATT ACGATCCTCG TCCCGATTTC AAGCCCATCA TTCCACTACC CGATGAAGTG 
GAGGTTCGAA CAGGGGAGGA GGGCGAGGAT ATCAAGTTTA CCAGCCGAGC CAAGCTCTTT 
CGATATGTGG ACAAGGAGTG GAAGGAGCGC GGGACAGGGG TGATCAAAAT TTTATGCGAC 
AAAGCCACCG GCGTTTCGCG AGTCTTGATG CGTCGCGATC AAACTCACAA AGTGTGTGCT 
AATCACACAA TCACTGCAGA CATTACGATA AATGTTGCCA ACCAGGACAA GGATAAGAAA 
TCCCTTCTGT GGGCGGCAAA TGATTTTGCG GATGAGCAAG TTACATTGGA GAGATTCCTG 
GTGCGTTTCA AAACAGGGGA GTTGGCTGAA GAGTTCAGGG TGGCTTTTAC AAAGGCAAGC 
GAGGCCGCCA AGTCGAAGGA AACCGTGAAA CCGACGGTAA ACACAGCGGA GAAAGGATCA 
ACTGCGACTG CTCCAGCGGC ATTCAAGAGC TTTGTAACCA GCACCCCAGC AGCCAATTCG 
CTAATCAATA AGCCTCAGGA GCAGACAAAA ACTCAGCCTA ATCCGGACCC ACCAGCAACA 
GCAGCTAAGT CACTGTTTGG AACCCTGTCA GTCAGTGCTG CTCCTGCAAC ATCTGCGCCA 
GCCTCTGCCA CACCATTTGC CAGCTTTAGC TTCACGCCGA ATGGGTCATC AGGATTTGGA 
ACTTCCACCG CCTCTCCGTT CGGAAACCTT TCTTTTGGTA CCGCTTCTGC GGTGGGCAGC 
GGCAACAACA CTACTTTATT CACCACGGCT TTGATCAAGG ACAATACAGT TCAAGGCAAA 
ACGCTTCAGC AGGAGTCACA ATTAAACAAG TCAAACTCCT CGGACGCTGA GGAGGAGTAT 
GTGCCCACCG CTCAGTTCGT GCCTGTTATT GCCCTGCCCG ATATAGTGGA GGTGGTCACC 
GGCGAAGAGA ATGAGGATGT GCTCTTCGAG CACCGTGCCA AGCTTCTGCG TTGGGATAAG 
GAAGCGAATG AGTGGAAGGA GCGCGGCCTT GGTAATATGA AGCTCCTTCG GGATCGCACT 
GATCCCAACA AGGTTCGCCT GCTTATGCGG CGCGAGCAGG TCCATAAGCT GTGCTGCAAT 
CAGCGACTGT TGCCTGAGAC CAAGTTCACT TATGCAACGA ACTGTAAAGC GGCCGTCACA 
TGGGGTGCTC AGGATTATTC CGATGAGGAG TTAACCACCG CATTGCTCGC TGTGCGATTT 
AAGTCGCAAG ACATCTGTCA GCAATTCCTC GAGGCCGTGC AAAAGGCTCA ACAAAGTATT 
GGAAATGAAC CCAAAAAGGA GGAGGTCCCT TCTGCCGCTG GAGAAAAGGA GAAGCCAATT 
AAAGGCTTTG GAGATGCCTT TAAGCCTAAG GCAGGCAGTT GGAACTGCCA AGCCTGCTAT 
ACCAACAATG GCCAGGATCA GCTCTACTGC TTGGCCTGCC AGGAGCCCAA AGATGCCACT 
GTTCCCCCCA AGCAATCGGG GTTAGACCAA GGGAACGCGT TAAATCTTAC CACTAGCTCC 
TCAAACAAGT TTTCGTTTGG CTTTGCCTCC TCAGCGACTC TTCCAGCCAC AGGTGGATTT 
AGTTTCGGTG GCGCCACACA GCCAAAGGAG AAGCCAGCAG TGGCAGTTGT TACCGCCTCG 
GCTTCGGCAC CTACGTCAGT TCAAACGGCA GCCCTTGGAT TTGGAAAGTC TTCAATGACT 
TCTGGATTCG GGGACGCCTT CAAACCTGCA GTCGGTAGTT GGTCATGTAG CGCATGCTAT 
GTGAACAATC CCGGAGAGTC TCTTTACTGC AGCGCCTGTG ATGCACCCAA GAATGACACG 
GTACCACAGA AGGAGAAGTC ACTAGGATCG GGACTTAATC TGCCACCTAC CTCCAAGTTT 
AGCTTTGGTT TCGGAGCCGC AGCGGCTGGT GACAAAGATC AGGCTGGCGA TGGAGCCACG 
TTCAACTTCG CTGCGATGCC CGCAGCTGTT GCTCCGACTA CCTCAATTGG ATCCAGCAGC 
TTCACCTTCT CCATGACCAA GCCCAAGCCC GACCAGCAGC AGCCAAATAG TACAGCAGCT 
AAGGAGGATG AAGATAACGA TTCGCAAGAG GTGGAGGAGG AGGAGAACAA CACTTACTTT 
TCACCAGTAA TCCCATTACC CGATAAGATT GATGTAAAAA CAGGTGAGGA GGACGAGGAG 
CTATTGTATG TGCATAAGGC CAAGTTGTAC CGCCTAAACG AGAGCGATTG GAAAGAGCGC 
GGCTTGGGGG ATGTGAAGAT CCTGCGCCAT AGGCAGACCA AAAAGCTGCG CGTGGTCATG 
CGTCGGGAGC AGGTGTTCAA GATCTGTCTT AACCATGTGC TTAACGAGAA TGTGGTGTAC 
AGGGAGAAGA CTGAAACGTC GTGGATGTTT GCTGTACACG ATTTTAGCGA GGGCGAGAGT 
GTCCTAGAGC GATTCACTTT GCGTTTTAAA AACAAGGAGG TGGCACAAGG ATTTATGGAA 
GCGATCAAAA ATGCTCTTAA TGAAACCGCA AAGCCGATAG AAGATAGCCC AGTGGTCGGC 
TCCGTTTCCC AATCGACGGA AGCAAATAAA CCGTCCCAAA AAAATGATGG GGCGGCAAAG 
AGCAGAGGAG GCGAATCGGA AGTACTTGAT GTTGGCAAAA CCTCTTCTCC ATTGGCCAAG 
CCTAACGATT ACCAAACACC TGCCACCATA TTGTTCAAAG GCAGTTCGCT GAGCAGAAAT 
AACTCTTCGG CTTCTGAGGC AAGCAAAACG CCTAGTTCAG CATTCATTTT CGGTAGCACG 
GATAAATCGG AGCCTGGAAA GGATGCTGGC CCATTAGCTA ATCTTCAAAA ACTAGCATCA 
GGCGAAGGAC AAGGAAACGT TTTGGGATCT ATATTCCGTA GCGGCTCGTC AAACGAAAAT 
TCGTCTGACG GTTCTGTCAA GTTCTTTTTC GGGGGTGGAA ATAAAGCGGC TGAGCAACAG 
AAGAAAGATT CCTCAGAGTC TGTCTTCGGA GGCAATAAAG CGGATTCCCA ATCGCCAGCG 
ACCCAAGAAG CACCGAAGTT GGCCTTCGGT GGAATTGCCG CACCTGTGTT CGGAGACGCC 
AATCCTTTTG GAGGCCACAA AGTTAATTTG CAAAAATCAG ATGGCAAGGA AGAACCAAAA 
TCTATCATTG GCGGAACCCC ATTGTTATTT GGAGGGTCCA ACGCCTTTGG AATACCCAAA 
ATTGAAACCC AGTCACCTGC TAAAGACTTT GTGTTTGGCA GTGCACCCGC TTTTGGACAA 
ATGGCAACCT TCTCATTTAC AGCTGCAAAA AATGAAAAGG AAAAGGACAT AACCTCGAAT 
AACACCACTG ATCTTAAAGC CGAAGGCAAG GAAAAGAAGG AGTTGGTGCC GGAGACGACC 
AGCACATTTG CCGATTTGGC CAAGACAGGC AGCACGTTTG CAGATTTGGC AAGCAATCCG 
GGCGGCACTT TTGCGGACTT AGCGAACAAG ACGGGCAACG ACTTTGCCAA CCTGTCGGCC 
AATAGCCAGG GCACTACCGT GGGATTCAAC AAGTCCGCCG GGGGCGGCTT TTATAACCTC 
ACGCATCAGA ACGCTTTCAA GAACTTCGAG TCGCCGCAAG CCACAGAAGA GTGCGATGAT 
GACGGCGACG CGACCACTGA TGACAACTAC GATCCGCACT ATGATGCCAT TGTAGAGCTG 
CCGGATGAGA TAGTTGTCAC CACGGGAGAG GAGAACGAGA CTAAGCTGTT TGGCGAGCGG 
GCGAAGCTTT ATCGCTATGA CGCCGAATCG AAACAATGGA AGGAGCGAGG TGTCGGTGAG 
ATAAAGGTGC TGGAGCACCC GGAGCTGCAG ACATTCCGAC TGATCATGCG GCAGGAGCAG 
ATCCACAAGC TGGTGCTTAA CATGAATATC TCCGCCTCCC TGCAAATGGA TTACATGAAC 
GCGCAGATGA AGAGCTTCCT GTGGGCCGGC TACAACTACG CGGTGGACGC AGAGGGTAAA 
GTTGACACCG AGGGCGTCCT GGAACGCCTT GCCTGTCGAT TCGCCAAGGA AGAGATCGCC 
AGTGAGTTCC TCAACACGGT CAATTCGTGC ATAAAACGAG CCAAGGCCTT GCAAGGTGAT 
GAGGAGAACA AGAATGACGA CGCGCCGGAG GAGCAGGCGA GCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651361.2
CDS	284..8371
Translation	MFTTRKEVDAHVHKMLGKLQPGRERDIKGLAVARLYMKVQEYPKAIEYLNGYLRVRDDAVGHNMIATCYSRLNPPDVTEALQHYQRSIQIDPRQSEVVIDACELLVKENNASITECARYWLDQANSLDLSGNKQVFNLRMRVNLADSNGERDDTSGGDGEQNTLEILMYKELQARPQDVNIRIQLLRSYVEKMKIDQAFNYALKTELESKNCTSQSNEWYEQIWMVLFKIEMAKDVKKNWRFWHFALHTLDRLVQLSLEGSGLADSSKQLFRLDQYLFKFSTSIERSGDAPQRDLHQACIDHFTGQLLLHAVTLIFKREVLANKNKWMSTLRSALPLLLLGYQVRPIDDSSTNQWIKHCDAEQKQLIQMWRPQGAFRCAQLGRTLLGCLDRSQMEIKNDRENAEFDENKNSGNSMPGLFADSEELLASAHQQCLDKSWRSQIYQQLFTHAEHKLKDTSSHLVRNLRLQLPLFEWPNLAHIENYELQALVLPPHSLAQHVYLALGTDPNKLGDAPRVVFYEGFQRDVKQNLNYCGQDSISQVDVDLYLYATTIQTRRKLQIQREVYDSSNLGNRNAAARPHMMPFANLVGQLGAPEQSNWWDLVVRLNSNQLITEGNRAEQRAQLQHGLEAVRGVNGPKADAIIIFQLGKILNSRSDRSSLEARIDTLYRQGFSILRHQHNQQMESYVRVFKYGSAGSTAAWQDLQSLAEEAVTYFSEKMFRIGQYEQFLDEVRGLHLPMAYFLQSEACHHLEESSKLPRTSRDRYSERRRECLQKTQKLIKNDDKHPLIAAMHRHQQDRNSRGIDNSFGSPDVHNNSSAYEDAEDDFYSHAAFSANRSRRQLEVTPVTPIVMAQPSQEMEQAVKQISKSLCVLKDDVSVGMEAMRQDIKVLTEKFTGLEDLLKKIKISSRDTPTRDVDPAAALGLDDLFIIEDALAEHQQQQQHQQQQSHNQGAIHPVVPNPYTSGFYNGMPNTPSAQERFLQGPYGSPMFNQNQMYNYYAAQAQAQAQAQFLRTPPAPGSIPPPNMFGPRNPNFGLPSMFPPPTVPSVAPYIDAMGNFTQPPPSLIPPPAQPAAPPAPLNILESKPVVALPTPGFFNTTTPVFGASPIQVPQSKPLTVPTVPIPSTAPAPPIAGTVNPPATTAVPPPVHIPQVAPSVPAQPPAPAPVSVPSMFNRALNNQPVEKEPPANVVITSSDPLPKPTTASVQPTLSVTIPAQHIKPSLVQAPEQPAQSAQPAQPSVSGVGSLSFNFGSKSSESPFSFKTQVAKAAAEKQKEQEEAEQNQSGATDPNKTLPQDTSADDYDPRPDFKPIIPLPDEVEVRTGEEGEDIKFTSRAKLFRYVDKEWKERGTGVIKILCDKATGVSRVLMRRDQTHKVCANHTITADITINVANQDKDKKSLLWAANDFADEQVTLERFLVRFKTGELAEEFRVAFTKASEAAKSKETVKPTVNTAEKGSTATAPAAFKSFVTSTPAANSLINKPQEQTKTQPNPDPPATAAKSLFGTLSVSAAPATSAPASATPFASFSFTPNGSSGFGTSTASPFGNLSFGTASAVGSGNNTTLFTTALIKDNTVQGKTLQQESQLNKSNSSDAEEEYVPTAQFVPVIALPDIVEVVTGEENEDVLFEHRAKLLRWDKEANEWKERGLGNMKLLRDRTDPNKVRLLMRREQVHKLCCNQRLLPETKFTYATNCKAAVTWGAQDYSDEELTTALLAVRFKSQDICQQFLEAVQKAQQSIGNEPKKEEVPSAAGEKEKPIKGFGDAFKPKAGSWNCQACYTNNGQDQLYCLACQEPKDATVPPKQSGLDQGNALNLTTSSSNKFSFGFASSATLPATGGFSFGGATQPKEKPAVAVVTASASAPTSVQTAALGFGKSSMTSGFGDAFKPAVGSWSCSACYVNNPGESLYCSACDAPKNDTVPQKEKSLGSGLNLPPTSKFSFGFGAAAAGDKDQAGDGATFNFAAMPAAVAPTTSIGSSSFTFSMTKPKPDQQQPNSTAAKEDEDNDSQEVEEEENNTYFSPVIPLPDKIDVKTGEEDEELLYVHKAKLYRLNESDWKERGLGDVKILRHRQTKKLRVVMRREQVFKICLNHVLNENVVYREKTETSWMFAVHDFSEGESVLERFTLRFKNKEVAQGFMEAIKNALNETAKPIEDSPVVGSVSQSTEANKPSQKNDGAAKSRGGESEVLDVGKTSSPLAKPNDYQTPATILFKGSSLSRNNSSASEASKTPSSAFIFGSTDKSEPGKDAGPLANLQKLASGEGQGNVLGSIFRSGSSNENSSDGSVKFFFGGGNKAAEQQKKDSSESVFGGNKADSQSPATQEAPKLAFGGIAAPVFGDANPFGGHKVNLQKSDGKEEPKSIIGGTPLLFGGSNAFGIPKIETQSPAKDFVFGSAPAFGQMATFSFTAAKNEKEKDITSNNTTDLKAEGKEKKELVPETTSTFADLAKTGSTFADLASNPGGTFADLANKTGNDFANLSANSQGTTVGFNKSAGGGFYNLTHQNAFKNFESPQATEECDDDGDATTDDNYDPHYDAIVELPDEIVVTTGEENETKLFGERAKLYRYDAESKQWKERGVGEIKVLEHPELQTFRLIMRQEQIHKLVLNMNISASLQMDYMNAQMKSFLWAGYNYAVDAEGKVDTEGVLERLACRFAKEEIASEFLNTVNSCIKRAKALQGDEENKNDDAPEEQASS

Target ORF information:

RefSeq Version	NM_143104.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 358kD, transcript variant A (Nup358), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143104.3

ORF Insert Sequence:

ATGTTTACAA CGCGAAAAGA AGTGGACGCA CACGTCCACA AAATGTTGGG AAAACTTCAG 
CCGGGAAGAG AGCGCGATAT CAAAGGCTTG GCTGTAGCTC GATTGTACAT GAAAGTTCAG 
GAATACCCAA AAGCCATCGA GTATTTGAAC GGCTATCTCC GGGTGAGGGA CGACGCTGTA 
GGCCATAACA TGATTGCCAC TTGCTACAGT AGGCTGAACC CGCCCGATGT GACAGAGGCC 
CTGCAGCACT ACCAACGTTC TATTCAAATT GATCCGCGTC AGTCAGAGGT TGTCATTGAC 
GCCTGCGAAC TTCTGGTGAA GGAGAACAAC GCATCGATCA CGGAGTGCGC CAGGTACTGG 
TTGGATCAGG CTAATAGCCT TGATTTAAGC GGCAACAAGC AAGTCTTCAA CCTGCGAATG 
CGTGTAAATC TTGCGGACAG TAACGGCGAA AGAGATGACA CCAGTGGCGG AGACGGCGAG 
CAAAATACCC TCGAGATTCT TATGTACAAG GAGCTGCAGG CGCGTCCCCA GGATGTTAAT 
ATACGCATAC AACTGCTGCG CAGCTATGTT GAAAAAATGA AAATAGACCA GGCCTTCAAC 
TATGCTCTAA AAACGGAACT GGAGTCGAAA AACTGCACGA GCCAGTCGAA CGAATGGTAC 
GAGCAGATCT GGATGGTCCT TTTCAAGATA GAAATGGCCA AGGATGTAAA GAAGAACTGG 
CGATTTTGGC ACTTCGCCTT GCACACTCTC GATCGTCTCG TGCAGCTAAG CCTGGAGGGC 
AGTGGACTGG CAGACAGCAG CAAGCAGTTG TTCAGACTTG ATCAATATCT ATTCAAGTTT 
AGCACTTCGA TAGAGAGGTC AGGGGACGCT CCGCAGCGGG ATCTCCACCA GGCTTGTATT 
GACCACTTTA CCGGCCAGCT TCTGCTTCAC GCGGTGACAC TGATTTTCAA ACGTGAGGTT 
CTGGCAAACA AAAACAAATG GATGAGCACG CTTCGCTCCG CTTTGCCACT TCTGCTCCTT 
GGTTACCAGG TGCGACCGAT CGATGATTCG AGTACCAACC AATGGATAAA ACACTGCGAC 
GCGGAACAGA AGCAGTTGAT ACAGATGTGG CGCCCTCAGG GCGCCTTTCG ATGTGCCCAG 
CTTGGACGAA CCCTTTTGGG CTGCTTGGAC CGGTCACAAA TGGAAATTAA AAACGATAGA 
GAAAATGCCG AGTTTGATGA GAATAAGAAT TCGGGAAATT CTATGCCGGG ATTATTTGCC 
GACTCCGAAG AGCTGTTGGC CAGCGCTCAC CAGCAATGCT TGGACAAGAG CTGGCGAAGT 
CAAATTTACC AACAGCTCTT TACCCACGCC GAACACAAGC TAAAGGATAC GTCTTCGCAT 
CTGGTGCGGA ACCTGCGTCT GCAGCTGCCT CTTTTTGAGT GGCCGAATCT GGCGCATATC 
GAGAACTACG AACTGCAAGC GTTGGTGCTG CCGCCACACT CCTTAGCCCA ACATGTCTAC 
CTGGCTCTGG GCACCGACCC GAACAAACTT GGAGATGCGC CACGCGTGGT CTTTTATGAG 
GGTTTCCAAC GGGATGTTAA GCAGAATCTA AACTACTGTG GGCAAGATTC AATCAGCCAG 
GTAGATGTGG ACCTCTACCT TTATGCCACA ACCATTCAGA CCAGACGTAA ATTGCAAATC 
CAGCGTGAGG TGTATGACTC CTCCAATTTG GGAAACCGCA ATGCTGCCGC CCGGCCACAT 
ATGATGCCAT TTGCCAATCT AGTGGGTCAA CTAGGCGCAC CGGAGCAAAG TAACTGGTGG 
GATCTGGTGG TGCGACTTAA CTCGAACCAG CTTATCACTG AAGGCAATCG CGCCGAGCAA 
CGAGCTCAAC TACAACATGG ATTGGAAGCG GTGCGCGGCG TTAATGGCCC AAAAGCAGAT 
GCCATCATCA TTTTTCAATT GGGAAAGATT TTAAATTCCC GATCGGATCG TTCTTCTCTA 
GAAGCGCGTA TCGATACCCT GTACAGGCAA GGATTTTCAA TTTTACGCCA CCAACACAAC 
CAGCAGATGG AATCCTATGT CCGCGTCTTT AAGTACGGAT CCGCCGGCTC AACGGCAGCA 
TGGCAAGATC TTCAGTCGTT GGCTGAGGAA GCCGTCACCT ATTTTAGCGA GAAAATGTTT 
AGGATTGGAC AGTATGAGCA GTTTTTGGAC GAGGTCCGGG GACTTCATCT GCCCATGGCC 
TACTTTTTGC AATCGGAGGC GTGTCATCAT CTTGAGGAAT CTTCCAAGCT GCCTCGAACT 
TCCCGAGATC GTTACTCAGA GCGAAGACGT GAATGCCTGC AGAAAACGCA AAAGTTAATC 
AAAAATGATG ATAAGCACCC ACTCATAGCG GCCATGCATA GACATCAACA GGACCGCAAT 
AGTCGGGGTA TCGATAATAG CTTCGGCAGT CCGGATGTTC ATAACAATTC ATCCGCCTAC 
GAGGACGCAG AAGATGATTT CTATTCCCAT GCAGCCTTCT CTGCCAATCG ATCACGTCGT 
CAGTTAGAAG TCACCCCAGT CACTCCCATT GTTATGGCAC AACCGAGTCA GGAAATGGAG 
CAGGCTGTCA AGCAGATCAG CAAGTCACTG TGTGTGCTCA AGGACGATGT GAGTGTGGGA 
ATGGAAGCCA TGCGGCAGGA TATCAAAGTT CTTACTGAGA AGTTCACTGG CCTGGAGGAT 
CTGCTTAAGA AGATCAAGAT CAGCAGTCGA GACACGCCTA CACGAGATGT CGATCCCGCA 
GCTGCCTTAG GCCTGGACGA CCTCTTCATA ATTGAGGATG CACTTGCGGA GCATCAGCAG 
CAGCAACAGC ATCAGCAGCA GCAGAGCCAC AACCAAGGTG CAATACACCC AGTCGTACCA 
AATCCATATA CTTCTGGCTT CTACAACGGG ATGCCAAACA CGCCATCCGC CCAAGAGCGC 
TTTCTGCAGG GACCATACGG GAGTCCCATG TTTAACCAAA ACCAGATGTA CAACTACTAC 
GCTGCCCAAG CTCAGGCTCA AGCGCAAGCG CAGTTCTTGC GGACTCCTCC AGCTCCAGGA 
TCAATTCCAC CTCCGAATAT GTTTGGTCCT CGAAATCCAA ACTTTGGCCT GCCCAGTATG 
TTTCCCCCGC CGACCGTGCC TTCGGTAGCA CCTTACATTG ACGCCATGGG CAACTTTACC 
CAGCCTCCCC CAAGTTTGAT ACCTCCTCCC GCTCAGCCAG CAGCTCCTCC GGCTCCCTTG 
AATATTCTTG AGTCCAAGCC TGTGGTTGCA CTGCCAACTC CAGGATTCTT CAATACGACA 
ACACCGGTCT TTGGAGCTTC GCCCATTCAA GTTCCGCAGT CGAAACCATT AACTGTTCCA 
ACGGTGCCAA TACCTAGTAC TGCTCCTGCT CCACCAATTG CTGGAACTGT GAATCCACCG 
GCAACAACTG CTGTTCCGCC TCCTGTTCAT ATACCTCAAG TGGCTCCTTC TGTTCCTGCA 
CAGCCACCCG CACCCGCCCC TGTTAGTGTG CCGTCTATGT TTAACCGTGC GTTGAACAAT 
CAGCCCGTGG AAAAGGAGCC GCCAGCCAAT GTGGTCATAA CCAGTTCAGA TCCGTTGCCG 
AAACCCACGA CTGCCAGTGT CCAACCCACT TTAAGTGTGA CCATTCCAGC ACAGCATATC 
AAGCCAAGTT TGGTTCAAGC ACCGGAACAG CCGGCACAAT CGGCACAACC GGCACAGCCA 
TCGGTGTCAG GAGTAGGATC ACTCAGCTTT AACTTTGGCA GTAAGTCTTC CGAAAGTCCT 
TTTTCATTCA AGACACAGGT GGCTAAGGCA GCAGCCGAAA AACAAAAGGA ACAAGAGGAA 
GCTGAGCAAA ACCAAAGTGG GGCTACTGAT CCCAACAAAA CTCTGCCACA AGATACTTCA 
GCAGATGATT ACGATCCTCG TCCCGATTTC AAGCCCATCA TTCCACTACC CGATGAAGTG 
GAGGTTCGAA CAGGGGAGGA GGGCGAGGAT ATCAAGTTTA CCAGCCGAGC CAAGCTCTTT 
CGATATGTGG ACAAGGAGTG GAAGGAGCGC GGGACAGGGG TGATCAAAAT TTTATGCGAC 
AAAGCCACCG GCGTTTCGCG AGTCTTGATG CGTCGCGATC AAACTCACAA AGTGTGTGCT 
AATCACACAA TCACTGCAGA CATTACGATA AATGTTGCCA ACCAGGACAA GGATAAGAAA 
TCCCTTCTGT GGGCGGCAAA TGATTTTGCG GATGAGCAAG TTACATTGGA GAGATTCCTG 
GTGCGTTTCA AAACAGGGGA GTTGGCTGAA GAGTTCAGGG TGGCTTTTAC AAAGGCAAGC 
GAGGCCGCCA AGTCGAAGGA AACCGTGAAA CCGACGGTAA ACACAGCGGA GAAAGGATCA 
ACTGCGACTG CTCCAGCGGC ATTCAAGAGC TTTGTAACCA GCACCCCAGC AGCCAATTCG 
CTAATCAATA AGCCTCAGGA GCAGACAAAA ACTCAGCCTA ATCCGGACCC ACCAGCAACA 
GCAGCTAAGT CACTGTTTGG AACCCTGTCA GTCAGTGCTG CTCCTGCAAC ATCTGCGCCA 
GCCTCTGCCA CACCATTTGC CAGCTTTAGC TTCACGCCGA ATGGGTCATC AGGATTTGGA 
ACTTCCACCG CCTCTCCGTT CGGAAACCTT TCTTTTGGTA CCGCTTCTGC GGTGGGCAGC 
GGCAACAACA CTACTTTATT CACCACGGCT TTGATCAAGG ACAATACAGT TCAAGGCAAA 
ACGCTTCAGC AGGAGTCACA ATTAAACAAG TCAAACTCCT CGGACGCTGA GGAGGAGTAT 
GTGCCCACCG CTCAGTTCGT GCCTGTTATT GCCCTGCCCG ATATAGTGGA GGTGGTCACC 
GGCGAAGAGA ATGAGGATGT GCTCTTCGAG CACCGTGCCA AGCTTCTGCG TTGGGATAAG 
GAAGCGAATG AGTGGAAGGA GCGCGGCCTT GGTAATATGA AGCTCCTTCG GGATCGCACT 
GATCCCAACA AGGTTCGCCT GCTTATGCGG CGCGAGCAGG TCCATAAGCT GTGCTGCAAT 
CAGCGACTGT TGCCTGAGAC CAAGTTCACT TATGCAACGA ACTGTAAAGC GGCCGTCACA 
TGGGGTGCTC AGGATTATTC CGATGAGGAG TTAACCACCG CATTGCTCGC TGTGCGATTT 
AAGTCGCAAG ACATCTGTCA GCAATTCCTC GAGGCCGTGC AAAAGGCTCA ACAAAGTATT 
GGAAATGAAC CCAAAAAGGA GGAGGTCCCT TCTGCCGCTG GAGAAAAGGA GAAGCCAATT 
AAAGGCTTTG GAGATGCCTT TAAGCCTAAG GCAGGCAGTT GGAACTGCCA AGCCTGCTAT 
ACCAACAATG GCCAGGATCA GCTCTACTGC TTGGCCTGCC AGGAGCCCAA AGATGCCACT 
GTTCCCCCCA AGCAATCGGG GTTAGACCAA GGGAACGCGT TAAATCTTAC CACTAGCTCC 
TCAAACAAGT TTTCGTTTGG CTTTGCCTCC TCAGCGACTC TTCCAGCCAC AGGTGGATTT 
AGTTTCGGTG GCGCCACACA GCCAAAGGAG AAGCCAGCAG TGGCAGTTGT TACCGCCTCG 
GCTTCGGCAC CTACGTCAGT TCAAACGGCA GCCCTTGGAT TTGGAAAGTC TTCAATGACT 
TCTGGATTCG GGGACGCCTT CAAACCTGCA GTCGGTAGTT GGTCATGTAG CGCATGCTAT 
GTGAACAATC CCGGAGAGTC TCTTTACTGC AGCGCCTGTG ATGCACCCAA GAATGACACG 
GTACCACAGA AGGAGAAGTC ACTAGGATCG GGACTTAATC TGCCACCTAC CTCCAAGTTT 
AGCTTTGGTT TCGGAGCCGC AGCGGCTGGT GACAAAGATC AGGCTGGCGA TGGAGCCACG 
TTCAACTTCG CTGCGATGCC CGCAGCTGTT GCTCCGACTA CCTCAATTGG ATCCAGCAGC 
TTCACCTTCT CCATGACCAA GCCCAAGCCC GACCAGCAGC AGCCAAATAG TACAGCAGCT 
AAGGAGGATG AAGATAACGA TTCGCAAGAG GTGGAGGAGG AGGAGAACAA CACTTACTTT 
TCACCAGTAA TCCCATTACC CGATAAGATT GATGTAAAAA CAGGTGAGGA GGACGAGGAG 
CTATTGTATG TGCATAAGGC CAAGTTGTAC CGCCTAAACG AGAGCGATTG GAAAGAGCGC 
GGCTTGGGGG ATGTGAAGAT CCTGCGCCAT AGGCAGACCA AAAAGCTGCG CGTGGTCATG 
CGTCGGGAGC AGGTGTTCAA GATCTGTCTT AACCATGTGC TTAACGAGAA TGTGGTGTAC 
AGGGAGAAGA CTGAAACGTC GTGGATGTTT GCTGTACACG ATTTTAGCGA GGGCGAGAGT 
GTCCTAGAGC GATTCACTTT GCGTTTTAAA AACAAGGAGG TGGCACAAGG ATTTATGGAA 
GCGATCAAAA ATGCTCTTAA TGAAACCGCA AAGCCGATAG AAGATAGCCC AGTGGTCGGC 
TCCGTTTCCC AATCGACGGA AGCAAATAAA CCGTCCCAAA AAAATGATGG GGCGGCAAAG 
AGCAGAGGAG GCGAATCGGA AGTACTTGAT GTTGGCAAAA CCTCTTCTCC ATTGGCCAAG 
CCTAACGATT ACCAAACACC TGCCACCATA TTGTTCAAAG GCAGTTCGCT GAGCAGAAAT 
AACTCTTCGG CTTCTGAGGC AAGCAAAACG CCTAGTTCAG CATTCATTTT CGGTAGCACG 
GATAAATCGG AGCCTGGAAA GGATGCTGGC CCATTAGCTA ATCTTCAAAA ACTAGCATCA 
GGCGAAGGAC AAGGAAACGT TTTGGGATCT ATATTCCGTA GCGGCTCGTC AAACGAAAAT 
TCGTCTGACG GTTCTGTCAA GTTCTTTTTC GGGGGTGGAA ATAAAGCGGC TGAGCAACAG 
AAGAAAGATT CCTCAGAGTC TGTCTTCGGA GGCAATAAAG CGGATTCCCA ATCGCCAGCG 
ACCCAAGAAG CACCGAAGTT GGCCTTCGGT GGAATTGCCG CACCTGTGTT CGGAGACGCC 
AATCCTTTTG GAGGCCACAA AGTTAATTTG CAAAAATCAG ATGGCAAGGA AGAACCAAAA 
TCTATCATTG GCGGAACCCC ATTGTTATTT GGAGGGTCCA ACGCCTTTGG AATACCCAAA 
ATTGAAACCC AGTCACCTGC TAAAGACTTT GTGTTTGGCA GTGCACCCGC TTTTGGACAA 
ATGGCAACCT TCTCATTTAC AGCTGCAAAA AATGAAAAGG AAAAGGACAT AACCTCGAAT 
AACACCACTG ATCTTAAAGC CGAAGGCAAG GAAAAGAAGG AGTTGGTGCC GGAGACGACC 
AGCACATTTG CCGATTTGGC CAAGACAGGC AGCACGTTTG CAGATTTGGC AAGCAATCCG 
GGCGGCACTT TTGCGGACTT AGCGAACAAG ACGGGCAACG ACTTTGCCAA CCTGTCGGCC 
AATAGCCAGG GCACTACCGT GGGATTCAAC AAGTCCGCCG GGGGCGGCTT TTATAACCTC 
ACGCATCAGA ACGCTTTCAA GAACTTCGAG TCGCCGCAAG CCACAGAAGA GTGCGATGAT 
GACGGCGACG CGACCACTGA TGACAACTAC GATCCGCACT ATGATGCCAT TGTAGAGCTG 
CCGGATGAGA TAGTTGTCAC CACGGGAGAG GAGAACGAGA CTAAGCTGTT TGGCGAGCGG 
GCGAAGCTTT ATCGCTATGA CGCCGAATCG AAACAATGGA AGGAGCGAGG TGTCGGTGAG 
ATAAAGGTGC TGGAGCACCC GGAGCTGCAG ACATTCCGAC TGATCATGCG GCAGGAGCAG 
ATCCACAAGC TGGTGCTTAA CATGAATATC TCCGCCTCCC TGCAAATGGA TTACATGAAC 
GCGCAGATGA AGAGCTTCCT GTGGGCCGGC TACAACTACG CGGTGGACGC AGAGGGTAAA 
GTTGACACCG AGGGCGTCCT GGAACGCCTT GCCTGTCGAT TCGCCAAGGA AGAGATCGCC 
AGTGAGTTCC TCAACACGGT CAATTCGTGC ATAAAACGAG CCAAGGCCTT GCAAGGTGAT 
GAGGAGAACA AGAATGACGA CGCGCCGGAG GAGCAGGCGA GCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nup358 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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