dib cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dib
Entrez Gene ID	45282
Full Name	disembodied
Synonyms	302a1,CG12028,CYP302A1,Cyp,Cyp302a1,Dib,Dmel\CG12028,l(3)64Ak,l(3)SH14,l(3)dib
General protein information	Preferred Names disembodied Names CG12028 gene product from transcript CG12028-RA CG12028-PA cytochrome P450 dib-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64A5-64A5\|3-12 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_080071.3	NP_524810.2	disembodied

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	dib	NP_524810.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005992	XP_316034.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

codes for a mitochondrial cytochrome P450 that mediates the last two hydroxylation reactions in the ecdysteroidogenic pathway in Drosophila
Title: Molecular and biochemical characterization of two P450 enzymes in the ecdysteroidogenic pathway of Drosophila melanogaster.

The following dib gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dib cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19397	NM_080071.3 Latest version!	Drosophila melanogaster disembodied (dib), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dib ( NM_080071.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524810.2 Drosophila melanogaster disembodied (dib), mRNA.

Price & Availability↑

CloneID	OFa19397
Clone ID Related Accession (Same CDS sequence)	NM_080071.3
Accession Version	NM_080071.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	disembodied
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080071.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACCA AACTGTTAAA GATTAGCTGC ACCTCGAGGC AGTGCACCTT TGCCAAGCCG 
TATCAGGCGA TACCAGGACC ACGAGGACCC TTTGGAATGG GTAATCTATA CAATTACCTG 
CCCGGAATCG GATCCTATTC CTGGCTAAGA TTGCACCAAG CCGGCCAGGA TAAGTATGAG 
AAATATGGCG CAATTGTGCG GGAAACTATA GTTCCTGGGC AGGACATTGT CTGGTTGTAC 
GATCCCAAGG ACATAGCTTT GCTGCTCAAC GAGCGGGATT GTCCGCAGCG AAGAAGTCAC 
CTGGCACTGG CTCAATATCG CAAGAGCCGA CCGGATGTCT ATAAAACCAC CGGCTTGCTG 
CCCACCAATG GTCCGGAATG GTGGCGTATA CGTGCCCAGG TGCAAAAGGA GCTGAGTGCA 
CCAAAGAGTG TGCGGAACTT TGTTCGCCAA GTGGATGGAG TGACCAAGGA GTTCATTAGA 
TTTCTACAAG AATCTCGCAA TGGTGGTGCC ATTGATATGC TGCCCAAGCT CACCAGATTG 
AATTTGGAAT TAACCTGTTT GCTTACCTTT GGAGCTCGTC TGCAGTCTTT TACTGCCCAG 
GAACAAGATC CTAGATCCCG ATCCACACGC TTGATGGATG CGGCTGAGAC CACCAATAGC 
TGCATCCTGC CCACAGATCA GGGCCTCCAG CTGTGGCGAT TTCTGGAGAC ACCTAGCTTT 
CGCAAACTAA GCCAGGCCCA ATCATATATG GAGAGTGTGG CCCTGGAGCT AGTGGAGGAG 
AATGTTAGGA ATGGTTCAGT GGGATCTTCA CTGATCTCGG CTTATGTAAA AAATCCCGAG 
CTTGATCGCA GTGACGTGGT GGGCACTGCT GCAGATTTAC TCTTGGCTGG CATCGATACC 
ACTTCGTATG CCTCGGCATT TCTGCTCTAT CACATAGCAC GAAATCCGGA GGTGCAGCAA 
AAACTGCACG AGGAGGCCAG GAGAGTGCTT CCGAGTGCCA AGGACGAGCT ATCCATGGAT 
GCCCTACGAA CAGATATCAC CTATACGAGG GCTGTCCTCA AGGAATCACT ACGCTTGAAT 
CCCATTGCCG TGGGCGTGGG CAGGATTCTT AATCAGGATG CGATCTTCAG TGGCTACTTT 
GTGCCAAAGG GGACCACCGT GGTTACCCAG AACATGGTAG CCTGTCGGCT GGAGCAGCAC 
TTCCAGGATC CCCTGCGCTT CCAACCAGAT CGATGGCTCC AGCACCGTAG TGCCCTCAAT 
CCCTATCTGG TCCTTCCCTT CGGTCACGGA ATGCGGGCCT GCATTGCCCG CCGTTTGGCC 
GAGCAGAATA TGCACATTTT GCTTCTCAGG CTGCTGCGTG AATACGAATT GATTTGGAGC 
GGATCCGATG ATGAGATGGG TGTGAAGACC CTGTTGATAA ATAAACCCGA TGCTCCAGTG 
CTGATCGATC TGCGATTGCG TAGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524810.2
CDS	49..1518
Translation	MLTKLLKISCTSRQCTFAKPYQAIPGPRGPFGMGNLYNYLPGIGSYSWLRLHQAGQDKYEKYGAIVRETIVPGQDIVWLYDPKDIALLLNERDCPQRRSHLALAQYRKSRPDVYKTTGLLPTNGPEWWRIRAQVQKELSAPKSVRNFVRQVDGVTKEFIRFLQESRNGGAIDMLPKLTRLNLELTCLLTFGARLQSFTAQEQDPRSRSTRLMDAAETTNSCILPTDQGLQLWRFLETPSFRKLSQAQSYMESVALELVEENVRNGSVGSSLISAYVKNPELDRSDVVGTAADLLLAGIDTTSYASAFLLYHIARNPEVQQKLHEEARRVLPSAKDELSMDALRTDITYTRAVLKESLRLNPIAVGVGRILNQDAIFSGYFVPKGTTVVTQNMVACRLEQHFQDPLRFQPDRWLQHRSALNPYLVLPFGHGMRACIARRLAEQNMHILLLRLLREYELIWSGSDDEMGVKTLLINKPDAPVLIDLRLRRE

Target ORF information:

RefSeq Version	NM_080071.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster disembodied (dib), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080071.3

ORF Insert Sequence:

ATGTTGACCA AACTGTTAAA GATTAGCTGC ACCTCGAGGC AGTGCACCTT TGCCAAGCCG 
TATCAGGCGA TACCAGGACC ACGAGGACCC TTTGGAATGG GTAATCTATA CAATTACCTG 
CCCGGAATCG GATCCTATTC CTGGCTAAGA TTGCACCAAG CCGGCCAGGA TAAGTATGAG 
AAATATGGCG CAATTGTGCG GGAAACTATA GTTCCTGGGC AGGACATTGT CTGGTTGTAC 
GATCCCAAGG ACATAGCTTT GCTGCTCAAC GAGCGGGATT GTCCGCAGCG AAGAAGTCAC 
CTGGCACTGG CTCAATATCG CAAGAGCCGA CCGGATGTCT ATAAAACCAC CGGCTTGCTG 
CCCACCAATG GTCCGGAATG GTGGCGTATA CGTGCCCAGG TGCAAAAGGA GCTGAGTGCA 
CCAAAGAGTG TGCGGAACTT TGTTCGCCAA GTGGATGGAG TGACCAAGGA GTTCATTAGA 
TTTCTACAAG AATCTCGCAA TGGTGGTGCC ATTGATATGC TGCCCAAGCT CACCAGATTG 
AATTTGGAAT TAACCTGTTT GCTTACCTTT GGAGCTCGTC TGCAGTCTTT TACTGCCCAG 
GAACAAGATC CTAGATCCCG ATCCACACGC TTGATGGATG CGGCTGAGAC CACCAATAGC 
TGCATCCTGC CCACAGATCA GGGCCTCCAG CTGTGGCGAT TTCTGGAGAC ACCTAGCTTT 
CGCAAACTAA GCCAGGCCCA ATCATATATG GAGAGTGTGG CCCTGGAGCT AGTGGAGGAG 
AATGTTAGGA ATGGTTCAGT GGGATCTTCA CTGATCTCGG CTTATGTAAA AAATCCCGAG 
CTTGATCGCA GTGACGTGGT GGGCACTGCT GCAGATTTAC TCTTGGCTGG CATCGATACC 
ACTTCGTATG CCTCGGCATT TCTGCTCTAT CACATAGCAC GAAATCCGGA GGTGCAGCAA 
AAACTGCACG AGGAGGCCAG GAGAGTGCTT CCGAGTGCCA AGGACGAGCT ATCCATGGAT 
GCCCTACGAA CAGATATCAC CTATACGAGG GCTGTCCTCA AGGAATCACT ACGCTTGAAT 
CCCATTGCCG TGGGCGTGGG CAGGATTCTT AATCAGGATG CGATCTTCAG TGGCTACTTT 
GTGCCAAAGG GGACCACCGT GGTTACCCAG AACATGGTAG CCTGTCGGCT GGAGCAGCAC 
TTCCAGGATC CCCTGCGCTT CCAACCAGAT CGATGGCTCC AGCACCGTAG TGCCCTCAAT 
CCCTATCTGG TCCTTCCCTT CGGTCACGGA ATGCGGGCCT GCATTGCCCG CCGTTTGGCC 
GAGCAGAATA TGCACATTTT GCTTCTCAGG CTGCTGCGTG AATACGAATT GATTTGGAGC 
GGATCCGATG ATGAGATGGG TGTGAAGACC CTGTTGATAA ATAAACCCGA TGCTCCAGTG 
CTGATCGATC TGCGATTGCG TAGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dib cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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