FREM3 cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM3
Entrez Gene ID	101087833
Full Name	FRAS1 related extracellular matrix 3
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003984978.2	XP_003985027.1	FRAS1-related extracellular matrix protein 3 isoform X3
XM_019828920.1	XP_019684479.1	FRAS1-related extracellular matrix protein 3 isoform X1
XM_003984978.3	XP_003985027.1	FRAS1-related extracellular matrix protein 3
XM_019828922.1	XP_019684481.1	FRAS1-related extracellular matrix protein 3 isoform X4
XM_019828923.1	XP_019684482.1	FRAS1-related extracellular matrix protein 3 isoform X4
XM_019828921.1	XP_019684480.1	FRAS1-related extracellular matrix protein 3 isoform X2

Pathways from BioSystems
Gene Ontology

The following FREM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb41448	XM_019828920.1 Latest version!	Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFb41449	XM_019828921.1 Latest version!	Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFb41450	XM_019828923.1 Latest version!	Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFb41450	XM_019828922.1 Latest version!	Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFb05542	XM_003984978.3 Latest version!	Felis catus FRAS1 related extracellular matrix 3 (FREM3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OFb05542	XM_003984978.2	Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM3 ( XM_019828920.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019684479.1 Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X1, mRNA.

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CloneID	OFb41448
Clone ID Related Accession (Same CDS sequence)	XM_019828920.1
Accession Version	XM_019828920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	FRAS1-related extracellular matrix protein 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018726.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTTA TCCAGAGTCC AGTTTAAGGA GCCCAATTTA 
CAGGAGCACT GCCTGAAAAA GACAAGCAGC CAAGCACAGT CATTTTACTG GAGCAGAGGA 
GACACCAGCT GTCAGTCCAT ACACTGCTGT ACCCACCAGG TTTCAGCCCA GTTGATGATG 
GACCATGAGG AGCAGCCCAA CACTGATGAC TCCACAATCC ATTTTCTACC AGGGTACCAG 
AGAACTGGGA CAGTGGATTT GGAAGAAATG GACAGAGACT ATCACCAGCT CACAAGTAAA 
CTATACAGAC AACCTCTATG GGAATTATGG GCATCTGACT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019684479.1
CDS	731..7435
Translation	MAGTSRRPAGTPRQLAAALAYVLLSCPVLHGQVPSPGTEDHPALYWPTWGAPGLSSPDGPSVLIANPGLRVPVGRSLWLDPLRDLVIRVQPGDWCEVTVLEALPLSHGALSPGRFPCAFGPRQVQYTHFGSRSPGRARVRLQLRYDAPTYTLALPFTLAVDVVFPQPELVMRNRPLAVEKLRGWSHAIDKRVLDFASLASAGSAPSRCRLTLLPPQGGPLPKYGRLVDAMGAPLPRGKGVDCEAFVRAGVRYQHTATTLSPSRDYVPFMAELLGPEDRGVGSAEVLLREHFQLLVNIRDGVENTAPRPSFVALMMMEVNQFLLTALTPDALAAEDTESDPDDLVFNILNAPTTTGGHEGYVVSTDDPLGLPVSFFTQRELRELKIAYQPPTKKLDSEQLFQLELEVVDGDGATSDPFAFMVIMKPANTLAPVARYNRGLLLFEGQSKPLSSTHSLQISDNDNLEEVTVTAVRGLRHGQLMVLGAPAGCKHFTPVDLAAGRVVYQHDGSNTYSDNIIFRMEDGHHQVEFLFPVTIIPVDDEPPKVKANTGLSVTEGHVVQISPFVLSATDTDSEDSTIHFVLEDQPLEGKKEEGGRDLAPGSSYSSQYQGEMLLRQSEPPQSPEDEDWSYVEREGLYEKVVTEWLQQDIMGGKLFYRYLGPHSPQPVVARLTFRVQDDHEPPNLSNQHFFTVRVQPVDLKSPELFPGTTLEMTVQQYQLTHFQKKFLQYTDEDSDDQNLWYTLLTPPTDTDDNHQVLAGEIVLTDSPDMPIMRFTQAQVNLHQVAYQPPQKMLGVARRVVQFTYRVEDAAGNSVPGTFTLFLKPVDNQPPKVTNRGFTVLEGESFILSRNELGVIDPDTDVDQIVFILVWGPQHGHLYYLNKHMTPGELFMQADVINGSVSYQHSRDQTTNDTFHLEVSDGVHHIPITIQISVHPTTADKTPRISITGGPLLDVSIDVLENRAAEITMGIIHGKKKGTDGLMLSFILEDSPKLGTILVHGLPAERFTQEDLISGAVVYVHTGGEVGFQKQHDAFSLTLSKGSYQWLVGNSIVERVQVQVVVLPVDNVAPKVSVAESYIVDEGGKSPLTLQHLSIEDVDTPQDEILCTVTSQPASGYLENIAPAPGSKESRAGSPISAFSISDVQVRHINYVQSIHKGVEPQKDQFTFYCSDGINFSPNVVFPIIILPTNDEQPELFAGEFVVLEGMSLVIDTPLLNGADADLPQNELHFQLTALPQHGRIVQQLATGSQPIHSFTLQEIQEASTIVYEHDDSETTEDSFEVWLSDGKHVTHRKVPIVVILVDDETPQLTINDGLEVETRHTKVITNWVLKATDLDSDEKSLSFVLRSGPQQGLLQRLRKPRGEVRNNLTLGMNFTQDEINRGLICYTHTGQEGVLDIVKFDVTDGINPLIDRSFYITVGSLDRVSPEVVSKGITLTEGGRVTLTTNLLSTSDINSPNEQLHFRVTRAPSLGHLESSDHSGKPIASFTQLQLAGNKICYVHTSNDEIKMDSFEFQVTDGHNPVFRTFRIYIIDVDDKKPILTIHKLMLQEGESKQITPFELTVEDKDTPDDLLLFTITQLPIHGRILYNGHHPVTTFTKQDLNDNLISYWHDGSEATEDCFSLTVTDGTHADFYVFPDTVLETHKPQVMRIHISSLDNRLPQIAINRGASALKRLHTGHMSFLITSRSLRTEDQNSVHGLLKYKVTRGPEHGFIINTGLGNKSTRVFTQADIDESRICYVLNEGSKATKDLFYFSVEDNGGNKLTHQPFHLNWAWISLEKEYYIVDEDSTFLEVTLMRRGYLEETSFISIVTKDETAKKDKDFKGKSQKHVQFSPGQTTAKWRVRIMSDNKYEASETFQIILLNPVMAALEFPEMATVEIVDPEDESTVYIPEAEYKIEEHVGELLIPVRRSGDSSQELMVICSTHEGSASGSIPSTALSFSDYVSRPEDGASILHFDKDEVEKSCRVLIIDDSLYEEEESFSVSLSLPVGGRLGAQFPTTKVVILADPNDEPTLHFGNAEYHVDESAGSLEICVWRTGPDLSQASSVTVHSRKTEPASAEAGIDYVGISRNLGFAPGVHMQIFQVTILDDLGQPILEGPERFELVLQMPVGAVLGEPNKTTIFINDTITDLSRVQFKEPNLQEHCLKKTSSQAQSFYWSRGDTSCQSIHCCTHQVSAQLMMDHEEQPNTDDSTIHFLPGYQRTGTVDLEEMDRDYHQLTSKLYRQPLWELWASDS

Target ORF information:

RefSeq Version	XM_019828920.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019828920.1

ORF Insert Sequence:

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTTA TCCAGAGTCC AGTTTAAGGA GCCCAATTTA 
CAGGAGCACT GCCTGAAAAA GACAAGCAGC CAAGCACAGT CATTTTACTG GAGCAGAGGA 
GACACCAGCT GTCAGTCCAT ACACTGCTGT ACCCACCAGG TTTCAGCCCA GTTGATGATG 
GACCATGAGG AGCAGCCCAA CACTGATGAC TCCACAATCC ATTTTCTACC AGGGTACCAG 
AGAACTGGGA CAGTGGATTT GGAAGAAATG GACAGAGACT ATCACCAGCT CACAAGTAAA 
CTATACAGAC AACCTCTATG GGAATTATGG GCATCTGACT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM3 ( XM_019828921.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019684480.1 Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X2, mRNA.

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CloneID	OFb41449
Clone ID Related Accession (Same CDS sequence)	XM_019828921.1
Accession Version	XM_019828921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	FRAS1-related extracellular matrix protein 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018726.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTTA TCCAGAGTCC AGTTTAAGGA GCCCAATTTA 
CAGGAGCACT GCCTGAAAAA GACAAGCAGC CAAGCACAGT CATTTTACTG GAGCAGAGGA 
GACACCAGCT GTCAGTCCAT ACACTGCTGT ACCCACCAGG TTTCAGCCCA GTTGATGATG 
GACCATGAGG AGCAGCCCAA CACTGATGAC TCCACAATCC ATTTTCTACC AGATGGCCAA 
ACCTATTGTG TGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019684480.1
CDS	731..7348
Translation	MAGTSRRPAGTPRQLAAALAYVLLSCPVLHGQVPSPGTEDHPALYWPTWGAPGLSSPDGPSVLIANPGLRVPVGRSLWLDPLRDLVIRVQPGDWCEVTVLEALPLSHGALSPGRFPCAFGPRQVQYTHFGSRSPGRARVRLQLRYDAPTYTLALPFTLAVDVVFPQPELVMRNRPLAVEKLRGWSHAIDKRVLDFASLASAGSAPSRCRLTLLPPQGGPLPKYGRLVDAMGAPLPRGKGVDCEAFVRAGVRYQHTATTLSPSRDYVPFMAELLGPEDRGVGSAEVLLREHFQLLVNIRDGVENTAPRPSFVALMMMEVNQFLLTALTPDALAAEDTESDPDDLVFNILNAPTTTGGHEGYVVSTDDPLGLPVSFFTQRELRELKIAYQPPTKKLDSEQLFQLELEVVDGDGATSDPFAFMVIMKPANTLAPVARYNRGLLLFEGQSKPLSSTHSLQISDNDNLEEVTVTAVRGLRHGQLMVLGAPAGCKHFTPVDLAAGRVVYQHDGSNTYSDNIIFRMEDGHHQVEFLFPVTIIPVDDEPPKVKANTGLSVTEGHVVQISPFVLSATDTDSEDSTIHFVLEDQPLEGKKEEGGRDLAPGSSYSSQYQGEMLLRQSEPPQSPEDEDWSYVEREGLYEKVVTEWLQQDIMGGKLFYRYLGPHSPQPVVARLTFRVQDDHEPPNLSNQHFFTVRVQPVDLKSPELFPGTTLEMTVQQYQLTHFQKKFLQYTDEDSDDQNLWYTLLTPPTDTDDNHQVLAGEIVLTDSPDMPIMRFTQAQVNLHQVAYQPPQKMLGVARRVVQFTYRVEDAAGNSVPGTFTLFLKPVDNQPPKVTNRGFTVLEGESFILSRNELGVIDPDTDVDQIVFILVWGPQHGHLYYLNKHMTPGELFMQADVINGSVSYQHSRDQTTNDTFHLEVSDGVHHIPITIQISVHPTTADKTPRISITGGPLLDVSIDVLENRAAEITMGIIHGKKKGTDGLMLSFILEDSPKLGTILVHGLPAERFTQEDLISGAVVYVHTGGEVGFQKQHDAFSLTLSKGSYQWLVGNSIVERVQVQVVVLPVDNVAPKVSVAESYIVDEGGKSPLTLQHLSIEDVDTPQDEILCTVTSQPASGYLENIAPAPGSKESRAGSPISAFSISDVQVRHINYVQSIHKGVEPQKDQFTFYCSDGINFSPNVVFPIIILPTNDEQPELFAGEFVVLEGMSLVIDTPLLNGADADLPQNELHFQLTALPQHGRIVQQLATGSQPIHSFTLQEIQEASTIVYEHDDSETTEDSFEVWLSDGKHVTHRKVPIVVILVDDETPQLTINDGLEVETRHTKVITNWVLKATDLDSDEKSLSFVLRSGPQQGLLQRLRKPRGEVRNNLTLGMNFTQDEINRGLICYTHTGQEGVLDIVKFDVTDGINPLIDRSFYITVGSLDRVSPEVVSKGITLTEGGRVTLTTNLLSTSDINSPNEQLHFRVTRAPSLGHLESSDHSGKPIASFTQLQLAGNKICYVHTSNDEIKMDSFEFQVTDGHNPVFRTFRIYIIDVDDKKPILTIHKLMLQEGESKQITPFELTVEDKDTPDDLLLFTITQLPIHGRILYNGHHPVTTFTKQDLNDNLISYWHDGSEATEDCFSLTVTDGTHADFYVFPDTVLETHKPQVMRIHISSLDNRLPQIAINRGASALKRLHTGHMSFLITSRSLRTEDQNSVHGLLKYKVTRGPEHGFIINTGLGNKSTRVFTQADIDESRICYVLNEGSKATKDLFYFSVEDNGGNKLTHQPFHLNWAWISLEKEYYIVDEDSTFLEVTLMRRGYLEETSFISIVTKDETAKKDKDFKGKSQKHVQFSPGQTTAKWRVRIMSDNKYEASETFQIILLNPVMAALEFPEMATVEIVDPEDESTVYIPEAEYKIEEHVGELLIPVRRSGDSSQELMVICSTHEGSASGSIPSTALSFSDYVSRPEDGASILHFDKDEVEKSCRVLIIDDSLYEEEESFSVSLSLPVGGRLGAQFPTTKVVILADPNDEPTLHFGNAEYHVDESAGSLEICVWRTGPDLSQASSVTVHSRKTEPASAEAGIDYVGISRNLGFAPGVHMQIFQVTILDDLGQPILEGPERFELVLQMPVGAVLGEPNKTTIFINDTITDLSRVQFKEPNLQEHCLKKTSSQAQSFYWSRGDTSCQSIHCCTHQVSAQLMMDHEEQPNTDDSTIHFLPDGQTYCVH

Target ORF information:

RefSeq Version	XM_019828921.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019828921.1

ORF Insert Sequence:

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTTA TCCAGAGTCC AGTTTAAGGA GCCCAATTTA 
CAGGAGCACT GCCTGAAAAA GACAAGCAGC CAAGCACAGT CATTTTACTG GAGCAGAGGA 
GACACCAGCT GTCAGTCCAT ACACTGCTGT ACCCACCAGG TTTCAGCCCA GTTGATGATG 
GACCATGAGG AGCAGCCCAA CACTGATGAC TCCACAATCC ATTTTCTACC AGATGGCCAA 
ACCTATTGTG TGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM3 ( XM_019828922.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019684481.1 Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X4, mRNA.

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CloneID	OFb41450
Clone ID Related Accession (Same CDS sequence)	XM_019828922.1 , XM_019828923.1
Accession Version	XM_019828922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	FRAS1-related extracellular matrix protein 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018726.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTGT TTCAGCCCAG TTGATGATGG ACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019684481.1
CDS	730..7146
Translation	MAGTSRRPAGTPRQLAAALAYVLLSCPVLHGQVPSPGTEDHPALYWPTWGAPGLSSPDGPSVLIANPGLRVPVGRSLWLDPLRDLVIRVQPGDWCEVTVLEALPLSHGALSPGRFPCAFGPRQVQYTHFGSRSPGRARVRLQLRYDAPTYTLALPFTLAVDVVFPQPELVMRNRPLAVEKLRGWSHAIDKRVLDFASLASAGSAPSRCRLTLLPPQGGPLPKYGRLVDAMGAPLPRGKGVDCEAFVRAGVRYQHTATTLSPSRDYVPFMAELLGPEDRGVGSAEVLLREHFQLLVNIRDGVENTAPRPSFVALMMMEVNQFLLTALTPDALAAEDTESDPDDLVFNILNAPTTTGGHEGYVVSTDDPLGLPVSFFTQRELRELKIAYQPPTKKLDSEQLFQLELEVVDGDGATSDPFAFMVIMKPANTLAPVARYNRGLLLFEGQSKPLSSTHSLQISDNDNLEEVTVTAVRGLRHGQLMVLGAPAGCKHFTPVDLAAGRVVYQHDGSNTYSDNIIFRMEDGHHQVEFLFPVTIIPVDDEPPKVKANTGLSVTEGHVVQISPFVLSATDTDSEDSTIHFVLEDQPLEGKKEEGGRDLAPGSSYSSQYQGEMLLRQSEPPQSPEDEDWSYVEREGLYEKVVTEWLQQDIMGGKLFYRYLGPHSPQPVVARLTFRVQDDHEPPNLSNQHFFTVRVQPVDLKSPELFPGTTLEMTVQQYQLTHFQKKFLQYTDEDSDDQNLWYTLLTPPTDTDDNHQVLAGEIVLTDSPDMPIMRFTQAQVNLHQVAYQPPQKMLGVARRVVQFTYRVEDAAGNSVPGTFTLFLKPVDNQPPKVTNRGFTVLEGESFILSRNELGVIDPDTDVDQIVFILVWGPQHGHLYYLNKHMTPGELFMQADVINGSVSYQHSRDQTTNDTFHLEVSDGVHHIPITIQISVHPTTADKTPRISITGGPLLDVSIDVLENRAAEITMGIIHGKKKGTDGLMLSFILEDSPKLGTILVHGLPAERFTQEDLISGAVVYVHTGGEVGFQKQHDAFSLTLSKGSYQWLVGNSIVERVQVQVVVLPVDNVAPKVSVAESYIVDEGGKSPLTLQHLSIEDVDTPQDEILCTVTSQPASGYLENIAPAPGSKESRAGSPISAFSISDVQVRHINYVQSIHKGVEPQKDQFTFYCSDGINFSPNVVFPIIILPTNDEQPELFAGEFVVLEGMSLVIDTPLLNGADADLPQNELHFQLTALPQHGRIVQQLATGSQPIHSFTLQEIQEASTIVYEHDDSETTEDSFEVWLSDGKHVTHRKVPIVVILVDDETPQLTINDGLEVETRHTKVITNWVLKATDLDSDEKSLSFVLRSGPQQGLLQRLRKPRGEVRNNLTLGMNFTQDEINRGLICYTHTGQEGVLDIVKFDVTDGINPLIDRSFYITVGSLDRVSPEVVSKGITLTEGGRVTLTTNLLSTSDINSPNEQLHFRVTRAPSLGHLESSDHSGKPIASFTQLQLAGNKICYVHTSNDEIKMDSFEFQVTDGHNPVFRTFRIYIIDVDDKKPILTIHKLMLQEGESKQITPFELTVEDKDTPDDLLLFTITQLPIHGRILYNGHHPVTTFTKQDLNDNLISYWHDGSEATEDCFSLTVTDGTHADFYVFPDTVLETHKPQVMRIHISSLDNRLPQIAINRGASALKRLHTGHMSFLITSRSLRTEDQNSVHGLLKYKVTRGPEHGFIINTGLGNKSTRVFTQADIDESRICYVLNEGSKATKDLFYFSVEDNGGNKLTHQPFHLNWAWISLEKEYYIVDEDSTFLEVTLMRRGYLEETSFISIVTKDETAKKDKDFKGKSQKHVQFSPGQTTAKWRVRIMSDNKYEASETFQIILLNPVMAALEFPEMATVEIVDPEDESTVYIPEAEYKIEEHVGELLIPVRRSGDSSQELMVICSTHEGSASGSIPSTALSFSDYVSRPEDGASILHFDKDEVEKSCRVLIIDDSLYEEEESFSVSLSLPVGGRLGAQFPTTKVVILADPNDEPTLHFGNAEYHVDESAGSLEICVWRTGPDLSQASSVTVHSRKTEPASAEAGIDYVGISRNLGFAPGVHMQIFQVTILDDLGQPILEGPERFELVLQMPVGAVLGEPNKTTIFINDTITDCFSPVDDGP

Target ORF information:

RefSeq Version	XM_019828922.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019828922.1

ORF Insert Sequence:

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTGT TTCAGCCCAG TTGATGATGG ACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM3 ( XM_019828923.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019684482.1 Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X5, mRNA.

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CloneID	OFb41450
Clone ID Related Accession (Same CDS sequence)	XM_019828922.1 , XM_019828923.1
Accession Version	XM_019828923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	FRAS1-related extracellular matrix protein 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018726.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTGT TTCAGCCCAG TTGATGATGG ACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019684482.1
CDS	730..7146
Translation	MAGTSRRPAGTPRQLAAALAYVLLSCPVLHGQVPSPGTEDHPALYWPTWGAPGLSSPDGPSVLIANPGLRVPVGRSLWLDPLRDLVIRVQPGDWCEVTVLEALPLSHGALSPGRFPCAFGPRQVQYTHFGSRSPGRARVRLQLRYDAPTYTLALPFTLAVDVVFPQPELVMRNRPLAVEKLRGWSHAIDKRVLDFASLASAGSAPSRCRLTLLPPQGGPLPKYGRLVDAMGAPLPRGKGVDCEAFVRAGVRYQHTATTLSPSRDYVPFMAELLGPEDRGVGSAEVLLREHFQLLVNIRDGVENTAPRPSFVALMMMEVNQFLLTALTPDALAAEDTESDPDDLVFNILNAPTTTGGHEGYVVSTDDPLGLPVSFFTQRELRELKIAYQPPTKKLDSEQLFQLELEVVDGDGATSDPFAFMVIMKPANTLAPVARYNRGLLLFEGQSKPLSSTHSLQISDNDNLEEVTVTAVRGLRHGQLMVLGAPAGCKHFTPVDLAAGRVVYQHDGSNTYSDNIIFRMEDGHHQVEFLFPVTIIPVDDEPPKVKANTGLSVTEGHVVQISPFVLSATDTDSEDSTIHFVLEDQPLEGKKEEGGRDLAPGSSYSSQYQGEMLLRQSEPPQSPEDEDWSYVEREGLYEKVVTEWLQQDIMGGKLFYRYLGPHSPQPVVARLTFRVQDDHEPPNLSNQHFFTVRVQPVDLKSPELFPGTTLEMTVQQYQLTHFQKKFLQYTDEDSDDQNLWYTLLTPPTDTDDNHQVLAGEIVLTDSPDMPIMRFTQAQVNLHQVAYQPPQKMLGVARRVVQFTYRVEDAAGNSVPGTFTLFLKPVDNQPPKVTNRGFTVLEGESFILSRNELGVIDPDTDVDQIVFILVWGPQHGHLYYLNKHMTPGELFMQADVINGSVSYQHSRDQTTNDTFHLEVSDGVHHIPITIQISVHPTTADKTPRISITGGPLLDVSIDVLENRAAEITMGIIHGKKKGTDGLMLSFILEDSPKLGTILVHGLPAERFTQEDLISGAVVYVHTGGEVGFQKQHDAFSLTLSKGSYQWLVGNSIVERVQVQVVVLPVDNVAPKVSVAESYIVDEGGKSPLTLQHLSIEDVDTPQDEILCTVTSQPASGYLENIAPAPGSKESRAGSPISAFSISDVQVRHINYVQSIHKGVEPQKDQFTFYCSDGINFSPNVVFPIIILPTNDEQPELFAGEFVVLEGMSLVIDTPLLNGADADLPQNELHFQLTALPQHGRIVQQLATGSQPIHSFTLQEIQEASTIVYEHDDSETTEDSFEVWLSDGKHVTHRKVPIVVILVDDETPQLTINDGLEVETRHTKVITNWVLKATDLDSDEKSLSFVLRSGPQQGLLQRLRKPRGEVRNNLTLGMNFTQDEINRGLICYTHTGQEGVLDIVKFDVTDGINPLIDRSFYITVGSLDRVSPEVVSKGITLTEGGRVTLTTNLLSTSDINSPNEQLHFRVTRAPSLGHLESSDHSGKPIASFTQLQLAGNKICYVHTSNDEIKMDSFEFQVTDGHNPVFRTFRIYIIDVDDKKPILTIHKLMLQEGESKQITPFELTVEDKDTPDDLLLFTITQLPIHGRILYNGHHPVTTFTKQDLNDNLISYWHDGSEATEDCFSLTVTDGTHADFYVFPDTVLETHKPQVMRIHISSLDNRLPQIAINRGASALKRLHTGHMSFLITSRSLRTEDQNSVHGLLKYKVTRGPEHGFIINTGLGNKSTRVFTQADIDESRICYVLNEGSKATKDLFYFSVEDNGGNKLTHQPFHLNWAWISLEKEYYIVDEDSTFLEVTLMRRGYLEETSFISIVTKDETAKKDKDFKGKSQKHVQFSPGQTTAKWRVRIMSDNKYEASETFQIILLNPVMAALEFPEMATVEIVDPEDESTVYIPEAEYKIEEHVGELLIPVRRSGDSSQELMVICSTHEGSASGSIPSTALSFSDYVSRPEDGASILHFDKDEVEKSCRVLIIDDSLYEEEESFSVSLSLPVGGRLGAQFPTTKVVILADPNDEPTLHFGNAEYHVDESAGSLEICVWRTGPDLSQASSVTVHSRKTEPASAEAGIDYVGISRNLGFAPGVHMQIFQVTILDDLGQPILEGPERFELVLQMPVGAVLGEPNKTTIFINDTITDCFSPVDDGP

Target ORF information:

RefSeq Version	XM_019828923.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019828923.1

ORF Insert Sequence:

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTGT TTCAGCCCAG TTGATGATGG ACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM3 ( XM_003984978.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003985027.1 Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X3, mRNA.

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CloneID	OFb05542
Clone ID Related Accession (Same CDS sequence)	XM_003984978.2 , XM_003984978.3
Accession Version	XM_003984978.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	FRAS1-related extracellular matrix protein 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018726.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003984978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTGT AAGCAGAGTA CTTGTAGTTC CTTTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003985027.1
CDS	729..7148
Translation	MAGTSRRPAGTPRQLAAALAYVLLSCPVLHGQVPSPGTEDHPALYWPTWGAPGLSSPDGPSVLIANPGLRVPVGRSLWLDPLRDLVIRVQPGDWCEVTVLEALPLSHGALSPGRFPCAFGPRQVQYTHFGSRSPGRARVRLQLRYDAPTYTLALPFTLAVDVVFPQPELVMRNRPLAVEKLRGWSHAIDKRVLDFASLASAGSAPSRCRLTLLPPQGGPLPKYGRLVDAMGAPLPRGKGVDCEAFVRAGVRYQHTATTLSPSRDYVPFMAELLGPEDRGVGSAEVLLREHFQLLVNIRDGVENTAPRPSFVALMMMEVNQFLLTALTPDALAAEDTESDPDDLVFNILNAPTTTGGHEGYVVSTDDPLGLPVSFFTQRELRELKIAYQPPTKKLDSEQLFQLELEVVDGDGATSDPFAFMVIMKPANTLAPVARYNRGLLLFEGQSKPLSSTHSLQISDNDNLEEVTVTAVRGLRHGQLMVLGAPAGCKHFTPVDLAAGRVVYQHDGSNTYSDNIIFRMEDGHHQVEFLFPVTIIPVDDEPPKVKANTGLSVTEGHVVQISPFVLSATDTDSEDSTIHFVLEDQPLEGKKEEGGRDLAPGSSYSSQYQGEMLLRQSEPPQSPEDEDWSYVEREGLYEKVVTEWLQQDIMGGKLFYRYLGPHSPQPVVARLTFRVQDDHEPPNLSNQHFFTVRVQPVDLKSPELFPGTTLEMTVQQYQLTHFQKKFLQYTDEDSDDQNLWYTLLTPPTDTDDNHQVLAGEIVLTDSPDMPIMRFTQAQVNLHQVAYQPPQKMLGVARRVVQFTYRVEDAAGNSVPGTFTLFLKPVDNQPPKVTNRGFTVLEGESFILSRNELGVIDPDTDVDQIVFILVWGPQHGHLYYLNKHMTPGELFMQADVINGSVSYQHSRDQTTNDTFHLEVSDGVHHIPITIQISVHPTTADKTPRISITGGPLLDVSIDVLENRAAEITMGIIHGKKKGTDGLMLSFILEDSPKLGTILVHGLPAERFTQEDLISGAVVYVHTGGEVGFQKQHDAFSLTLSKGSYQWLVGNSIVERVQVQVVVLPVDNVAPKVSVAESYIVDEGGKSPLTLQHLSIEDVDTPQDEILCTVTSQPASGYLENIAPAPGSKESRAGSPISAFSISDVQVRHINYVQSIHKGVEPQKDQFTFYCSDGINFSPNVVFPIIILPTNDEQPELFAGEFVVLEGMSLVIDTPLLNGADADLPQNELHFQLTALPQHGRIVQQLATGSQPIHSFTLQEIQEASTIVYEHDDSETTEDSFEVWLSDGKHVTHRKVPIVVILVDDETPQLTINDGLEVETRHTKVITNWVLKATDLDSDEKSLSFVLRSGPQQGLLQRLRKPRGEVRNNLTLGMNFTQDEINRGLICYTHTGQEGVLDIVKFDVTDGINPLIDRSFYITVGSLDRVSPEVVSKGITLTEGGRVTLTTNLLSTSDINSPNEQLHFRVTRAPSLGHLESSDHSGKPIASFTQLQLAGNKICYVHTSNDEIKMDSFEFQVTDGHNPVFRTFRIYIIDVDDKKPILTIHKLMLQEGESKQITPFELTVEDKDTPDDLLLFTITQLPIHGRILYNGHHPVTTFTKQDLNDNLISYWHDGSEATEDCFSLTVTDGTHADFYVFPDTVLETHKPQVMRIHISSLDNRLPQIAINRGASALKRLHTGHMSFLITSRSLRTEDQNSVHGLLKYKVTRGPEHGFIINTGLGNKSTRVFTQADIDESRICYVLNEGSKATKDLFYFSVEDNGGNKLTHQPFHLNWAWISLEKEYYIVDEDSTFLEVTLMRRGYLEETSFISIVTKDETAKKDKDFKGKSQKHVQFSPGQTTAKWRVRIMSDNKYEASETFQIILLNPVMAALEFPEMATVEIVDPEDESTVYIPEAEYKIEEHVGELLIPVRRSGDSSQELMVICSTHEGSASGSIPSTALSFSDYVSRPEDGASILHFDKDEVEKSCRVLIIDDSLYEEEESFSVSLSLPVGGRLGAQFPTTKVVILADPNDEPTLHFGNAEYHVDESAGSLEICVWRTGPDLSQASSVTVHSRKTEPASAEAGIDYVGISRNLGFAPGVHMQIFQVTILDDLGQPILEGPERFELVLQMPVGAVLGEPNKTTIFINDTITDCKQSTCSSFD

Target ORF information:

RefSeq Version	XM_003984978.2
Organism	Felis catus(Domestic cat)
Definition	Felis catus FRAS1 related extracellular matrix 3 (FREM3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003984978.2

ORF Insert Sequence:

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTGT AAGCAGAGTA CTTGTAGTTC CTTTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM3 ( XM_003984978.3 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003985027.1 Felis catus FRAS1 related extracellular matrix 3 (FREM3), mRNA.

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CloneID	OFb05542
Clone ID Related Accession (Same CDS sequence)	XM_003984978.2 , XM_003984978.3
Accession Version	XM_003984978.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	FRAS1-related extracellular matrix protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018726.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_003984978.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTGT AAGCAGAGTA CTTGTAGTTC CTTTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003985027.1
CDS	750..7169
Translation	MAGTSRRPAGTPRQLAAALAYVLLSCPVLHGQVPSPGTEDHPALYWPTWGAPGLSSPDGPSVLIANPGLRVPVGRSLWLDPLRDLVIRVQPGDWCEVTVLEALPLSHGALSPGRFPCAFGPRQVQYTHFGSRSPGRARVRLQLRYDAPTYTLALPFTLAVDVVFPQPELVMRNRPLAVEKLRGWSHAIDKRVLDFASLASAGSAPSRCRLTLLPPQGGPLPKYGRLVDAMGAPLPRGKGVDCEAFVRAGVRYQHTATTLSPSRDYVPFMAELLGPEDRGVGSAEVLLREHFQLLVNIRDGVENTAPRPSFVALMMMEVNQFLLTALTPDALAAEDTESDPDDLVFNILNAPTTTGGHEGYVVSTDDPLGLPVSFFTQRELRELKIAYQPPTKKLDSEQLFQLELEVVDGDGATSDPFAFMVIMKPANTLAPVARYNRGLLLFEGQSKPLSSTHSLQISDNDNLEEVTVTAVRGLRHGQLMVLGAPAGCKHFTPVDLAAGRVVYQHDGSNTYSDNIIFRMEDGHHQVEFLFPVTIIPVDDEPPKVKANTGLSVTEGHVVQISPFVLSATDTDSEDSTIHFVLEDQPLEGKKEEGGRDLAPGSSYSSQYQGEMLLRQSEPPQSPEDEDWSYVEREGLYEKVVTEWLQQDIMGGKLFYRYLGPHSPQPVVARLTFRVQDDHEPPNLSNQHFFTVRVQPVDLKSPELFPGTTLEMTVQQYQLTHFQKKFLQYTDEDSDDQNLWYTLLTPPTDTDDNHQVLAGEIVLTDSPDMPIMRFTQAQVNLHQVAYQPPQKMLGVARRVVQFTYRVEDAAGNSVPGTFTLFLKPVDNQPPKVTNRGFTVLEGESFILSRNELGVIDPDTDVDQIVFILVWGPQHGHLYYLNKHMTPGELFMQADVINGSVSYQHSRDQTTNDTFHLEVSDGVHHIPITIQISVHPTTADKTPRISITGGPLLDVSIDVLENRAAEITMGIIHGKKKGTDGLMLSFILEDSPKLGTILVHGLPAERFTQEDLISGAVVYVHTGGEVGFQKQHDAFSLTLSKGSYQWLVGNSIVERVQVQVVVLPVDNVAPKVSVAESYIVDEGGKSPLTLQHLSIEDVDTPQDEILCTVTSQPASGYLENIAPAPGSKESRAGSPISAFSISDVQVRHINYVQSIHKGVEPQKDQFTFYCSDGINFSPNVVFPIIILPTNDEQPELFAGEFVVLEGMSLVIDTPLLNGADADLPQNELHFQLTALPQHGRIVQQLATGSQPIHSFTLQEIQEASTIVYEHDDSETTEDSFEVWLSDGKHVTHRKVPIVVILVDDETPQLTINDGLEVETRHTKVITNWVLKATDLDSDEKSLSFVLRSGPQQGLLQRLRKPRGEVRNNLTLGMNFTQDEINRGLICYTHTGQEGVLDIVKFDVTDGINPLIDRSFYITVGSLDRVSPEVVSKGITLTEGGRVTLTTNLLSTSDINSPNEQLHFRVTRAPSLGHLESSDHSGKPIASFTQLQLAGNKICYVHTSNDEIKMDSFEFQVTDGHNPVFRTFRIYIIDVDDKKPILTIHKLMLQEGESKQITPFELTVEDKDTPDDLLLFTITQLPIHGRILYNGHHPVTTFTKQDLNDNLISYWHDGSEATEDCFSLTVTDGTHADFYVFPDTVLETHKPQVMRIHISSLDNRLPQIAINRGASALKRLHTGHMSFLITSRSLRTEDQNSVHGLLKYKVTRGPEHGFIINTGLGNKSTRVFTQADIDESRICYVLNEGSKATKDLFYFSVEDNGGNKLTHQPFHLNWAWISLEKEYYIVDEDSTFLEVTLMRRGYLEETSFISIVTKDETAKKDKDFKGKSQKHVQFSPGQTTAKWRVRIMSDNKYEASETFQIILLNPVMAALEFPEMATVEIVDPEDESTVYIPEAEYKIEEHVGELLIPVRRSGDSSQELMVICSTHEGSASGSIPSTALSFSDYVSRPEDGASILHFDKDEVEKSCRVLIIDDSLYEEEESFSVSLSLPVGGRLGAQFPTTKVVILADPNDEPTLHFGNAEYHVDESAGSLEICVWRTGPDLSQASSVTVHSRKTEPASAEAGIDYVGISRNLGFAPGVHMQIFQVTILDDLGQPILEGPERFELVLQMPVGAVLGEPNKTTIFINDTITDCKQSTCSSFD

Target ORF information:

RefSeq Version	XM_003984978.3
Organism	Felis catus(domestic cat)
Definition	Felis catus FRAS1 related extracellular matrix 3 (FREM3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003984978.3

ORF Insert Sequence:

ATGGCTGGGA CTTCTCGGCG CCCCGCTGGG ACGCCCCGGC AGCTTGCCGC GGCGCTCGCC 
TACGTGCTCC TGAGCTGCCC CGTGCTACAT GGACAGGTGC CCTCGCCTGG GACTGAAGAC 
CACCCTGCGC TTTACTGGCC CACCTGGGGC GCACCGGGTC TCAGTAGCCC CGACGGCCCT 
AGCGTCCTGA TCGCCAACCC CGGACTCCGG GTTCCTGTGG GCCGCTCGCT TTGGCTCGAC 
CCGCTCCGGG ACCTGGTGAT TCGAGTGCAG CCGGGGGACT GGTGCGAGGT GACGGTGCTG 
GAAGCCCTGC CGCTGAGCCA CGGCGCGCTC TCCCCCGGCC GCTTCCCCTG CGCCTTCGGA 
CCCCGCCAGG TCCAGTACAC CCACTTCGGC TCCCGAAGCC CCGGGCGCGC TCGGGTGCGA 
CTGCAGCTGC GCTACGACGC CCCGACTTAC ACGCTAGCAC TGCCCTTCAC GCTGGCTGTG 
GACGTGGTGT TTCCCCAGCC GGAGCTAGTG ATGCGCAACA GGCCTTTGGC GGTGGAGAAG 
CTGCGGGGCT GGAGCCATGC CATCGACAAG AGAGTGCTGG ACTTTGCGTC CCTGGCGTCC 
GCAGGCTCTG CCCCCAGCAG GTGCCGGCTC ACCCTTCTTC CTCCCCAGGG CGGCCCACTG 
CCCAAGTATG GGCGTTTGGT GGACGCCATG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTTGTCAG AGCTGGGGTG CGCTACCAGC ATACCGCCAC CACCCTGTCG 
CCCAGTCGGG ACTATGTGCC CTTTATGGCG GAGCTGCTAG GGCCAGAAGA CAGAGGCGTG 
GGGTCAGCCG AGGTGCTGCT CCGTGAGCAT TTCCAGCTGC TGGTGAACAT CCGCGACGGA 
GTAGAGAACA CCGCACCTCG GCCCAGTTTT GTGGCCCTGA TGATGATGGA GGTCAATCAG 
TTCTTGCTGA CAGCCCTGAC CCCTGACGCA CTGGCTGCGG AAGACACGGA GTCTGACCCT 
GATGACCTGG TCTTCAACAT TCTCAATGCC CCCACAACCA CAGGAGGGCA CGAGGGGTAC 
GTGGTCAGCA CTGACGACCC CCTGGGCCTT CCAGTCTCCT TCTTCACCCA GCGGGAGCTT 
CGGGAACTGA AAATTGCCTA TCAGCCCCCT ACAAAGAAAT TGGATTCAGA GCAGCTCTTC 
CAGTTGGAGC TGGAGGTGGT GGATGGAGAT GGTGCCACCT CAGACCCCTT TGCCTTCATG 
GTGATAATGA AGCCCGCGAA CACTCTGGCC CCAGTGGCTA GATATAACCG AGGACTGCTG 
CTATTTGAAG GTCAGTCCAA GCCCCTGTCC AGCACCCACA GCCTTCAGAT CAGTGATAAC 
GATAACCTGG AAGAAGTGAC AGTAACTGCA GTTAGGGGCT TAAGACATGG GCAGCTGATG 
GTGCTTGGGG CACCCGCTGG GTGCAAGCAT TTCACACCGG TGGACCTGGC AGCAGGGCGA 
GTGGTTTACC AGCATGATGG CAGCAACACC TACAGTGACA ACATCATCTT CCGGATGGAG 
GATGGACACC ACCAGGTGGA ATTCCTCTTC CCCGTCACCA TCATACCTGT CGATGATGAA 
CCACCAAAGG TCAAGGCCAA CACCGGGCTC TCTGTTACAG AAGGACACGT GGTCCAGATT 
TCCCCCTTTG TCCTGAGTGC TACTGATACT GACTCTGAGG ACTCAACCAT CCACTTTGTG 
CTGGAGGACC AGCCTCTGGA AGGGAAAAAG GAAGAGGGAG GGCGGGATCT AGCCCCTGGT 
TCCTCCTACT CCAGTCAGTA CCAGGGGGAG ATGCTGCTGC GGCAGAGTGA ACCACCTCAG 
TCCCCTGAAG ATGAGGACTG GTCTTATGTG GAAAGAGAAG GACTTTATGA GAAGGTGGTG 
ACTGAATGGC TTCAGCAAGA CATAATGGGA GGGAAACTCT TCTACCGCTA TCTTGGACCC 
CATAGCCCTC AACCTGTAGT GGCCCGGCTG ACTTTCCGTG TACAGGATGA CCATGAGCCA 
CCCAATCTAT CCAACCAACA CTTCTTCACT GTCAGGGTCC AACCAGTGGA TCTGAAGAGT 
CCGGAACTAT TTCCAGGAAC TACTCTAGAA ATGACTGTGC AACAATACCA ACTCACTCAC 
TTCCAGAAGA AATTCCTCCA ATATACTGAT GAGGACTCAG ATGACCAGAA CCTGTGGTAC 
ACCCTGCTGA CACCCCCAAC TGACACAGAT GACAACCACC AAGTCCTGGC TGGAGAAATT 
GTCCTCACTG ATTCGCCAGA CATGCCCATC ATGCGCTTCA CCCAGGCCCA GGTAAATCTG 
CACCAAGTTG CCTATCAACC CCCACAGAAG ATGCTGGGTG TCGCACGACG GGTTGTGCAG 
TTCACCTACC GAGTGGAGGA TGCTGCAGGC AATAGTGTGC CTGGCACATT CACGTTATTC 
CTGAAGCCTG TGGACAATCA GCCTCCCAAA GTCACCAACA GAGGCTTTAC TGTCTTAGAG 
GGAGAAAGTT TTATCCTCAG CAGGAATGAA CTGGGTGTCA TAGATCCTGA CACAGATGTT 
GACCAGATTG TCTTCATATT AGTCTGGGGT CCCCAACATG GACACTTGTA CTACTTAAAC 
AAACATATGA CCCCAGGAGA GCTTTTCATG CAAGCTGATG TAATTAATGG GAGTGTCTCT 
TACCAGCACA GCAGAGACCA GACTACCAAT GACACCTTCC ATCTGGAGGT AAGTGACGGG 
GTTCACCACA TACCCATCAC CATCCAGATT TCTGTGCATC CCACTACAGC TGACAAAACT 
CCCAGGATCT CTATTACAGG TGGTCCGTTA TTAGATGTTT CCATTGATGT ATTAGAGAAC 
AGAGCTGCTG AGATCACCAT GGGCATCATA CATGGCAAAA AGAAGGGCAC AGATGGCTTG 
ATGTTGTCTT TCATTTTGGA AGATAGCCCA AAATTGGGTA CTATTCTCGT GCATGGTTTA 
CCTGCAGAAC GATTCACCCA AGAGGACCTC ATCAGTGGGG CAGTAGTCTA TGTCCACACT 
GGGGGTGAAG TTGGTTTCCA GAAACAACAT GATGCCTTCA GCCTCACCCT CTCCAAGGGT 
TCTTATCAGT GGTTGGTGGG GAACAGCATA GTGGAAAGAG TGCAGGTGCA AGTGGTTGTG 
CTGCCTGTGG ACAATGTGGC ACCCAAAGTC TCAGTGGCAG AGTCTTATAT TGTTGATGAA 
GGTGGCAAGA GTCCCTTGAC CTTACAACAT CTCAGTATTG AAGATGTGGA CACACCCCAA 
GATGAAATCC TATGCACAGT GACCAGTCAG CCAGCCTCTG GCTACCTGGA AAACATTGCA 
CCAGCTCCTG GCTCAAAAGA ATCACGGGCT GGTAGCCCCA TCAGTGCTTT CTCCATCAGT 
GATGTCCAAG TGAGGCATAT CAATTATGTA CAGAGTATTC ACAAGGGGGT AGAGCCACAA 
AAAGATCAAT TCACCTTTTA TTGTTCTGAT GGCATCAACT TCTCCCCAAA TGTTGTCTTC 
CCCATAATCA TTTTACCCAC CAATGACGAG CAGCCTGAAC TTTTTGCTGG TGAGTTTGTG 
GTATTAGAGG GGATGAGCCT GGTCATAGAC ACCCCACTGC TCAATGGAGC AGATGCTGAC 
TTGCCACAGA ATGAGCTTCA CTTTCAGCTC ACGGCCCTCC CTCAGCATGG ACGAATCGTA 
CAGCAGCTGG CCACAGGCAG CCAGCCCATC CACAGTTTTA CCTTACAGGA GATCCAGGAG 
GCCTCCACCA TTGTGTATGA GCATGATGAC TCCGAGACCA CAGAGGACAG TTTTGAGGTC 
TGGCTGAGTG ACGGCAAGCA CGTTACCCAC AGGAAGGTTC CCATTGTGGT GATCCTAGTG 
GATGATGAAA CCCCTCAGCT GACCATTAAT GATGGGCTGG AAGTAGAGAC CAGACACACT 
AAGGTCATTA CAAATTGGGT CCTCAAAGCC ACAGATCTTG ACTCCGATGA GAAGAGCCTC 
AGTTTTGTCC TCCGTTCAGG GCCTCAACAA GGGCTTCTAC AGCGGCTGAG AAAACCCAGA 
GGGGAGGTGA GGAACAACCT CACCCTTGGG ATGAACTTTA CCCAGGATGA GATTAACAGA 
GGCCTCATCT GTTACACTCA CACAGGCCAA GAAGGAGTTC TTGACATTGT CAAATTTGAT 
GTGACTGATG GGATTAACCC TTTGATAGAC CGCTCCTTCT ACATCACCGT TGGCAGTTTA 
GACAGAGTCT CACCTGAGGT GGTTAGCAAA GGGATTACCC TGACAGAAGG TGGCAGAGTG 
ACCCTTACCA CCAATCTGCT TAGCACCAGC GACATCAACA GCCCTAATGA ACAGCTTCAC 
TTCAGAGTCA CACGGGCTCC TAGCCTGGGG CACTTGGAGA GCTCTGACCA CTCTGGGAAG 
CCCATTGCCT CTTTCACTCA GCTACAGTTG GCTGGCAACA AAATATGCTA TGTCCACACT 
TCAAATGATG AGATAAAGAT GGACAGCTTT GAGTTTCAAG TGACTGATGG ACACAACCCT 
GTGTTCCGAA CCTTCAGGAT CTACATCATA GATGTGGATG ATAAGAAACC TATCTTGACC 
ATCCACAAAC TAATGCTGCA AGAAGGGGAA AGCAAACAGA TCACTCCCTT TGAGTTAACA 
GTGGAAGATA AGGATACTCC AGATGATCTT CTCCTCTTTA CTATCACCCA GCTCCCCATC 
CATGGCAGGA TTTTGTATAA TGGCCACCAT CCTGTGACCA CCTTCACCAA GCAAGACCTG 
AATGACAACC TGATTAGCTA CTGGCATGAC GGCAGTGAGG CGACTGAAGA CTGTTTCTCT 
TTGACTGTGA CAGATGGCAC TCATGCTGAC TTCTATGTCT TCCCAGACAC TGTCCTAGAG 
ACACACAAAC CTCAGGTAAT GAGGATCCAC ATAAGTTCCC TAGATAACAG ACTCCCCCAG 
ATTGCCATTA ACAGAGGTGC ATCGGCCTTG AAGCGCCTTC ACACTGGACA CATGAGCTTC 
TTGATTACCA GCAGGTCTCT GAGGACAGAG GATCAGAACA GTGTCCATGG GCTCCTGAAG 
TATAAAGTGA CCAGAGGACC AGAGCATGGC TTCATTATCA ACACTGGCCT TGGGAACAAG 
AGTACGCGAG TGTTTACACA AGCTGACATT GATGAGAGCA GGATCTGCTA TGTGTTGAAT 
GAAGGCAGCA AAGCAACAAA GGACCTCTTC TACTTCTCTG TTGAAGACAA TGGAGGAAAT 
AAGCTAACAC ATCAACCTTT CCATCTGAAC TGGGCTTGGA TTTCTCTGGA GAAAGAATAC 
TACATTGTGG ATGAAGACTC CACGTTCTTA GAAGTGACCC TCATGCGCAG AGGGTATCTT 
GAAGAAACAT CCTTTATCAG CATTGTAACC AAAGATGAAA CTGCAAAGAA AGATAAAGAT 
TTTAAAGGAA AGTCCCAGAA ACATGTCCAA TTCAGTCCTG GACAGACTAC AGCCAAGTGG 
AGAGTGAGAA TTATGTCCGA CAATAAATAT GAGGCTTCGG AGACCTTCCA GATCATTCTG 
TTGAACCCTG TCATGGCTGC ACTGGAGTTT CCAGAAATGG CAACTGTTGA AATTGTAGAC 
CCTGAAGATG AATCAACAGT TTATATTCCA GAGGCAGAAT ACAAAATAGA GGAGCATGTT 
GGAGAACTTT TGATTCCTGT AAGGCGGTCT GGAGATTCTA GCCAAGAACT GATGGTCATT 
TGCTCTACAC ATGAAGGTTC TGCCTCAGGC TCCATTCCTT CAACTGCATT ATCTTTCTCT 
GACTACGTCT CCCGTCCAGA AGATGGCGCC AGCATCCTCC ACTTTGACAA GGATGAGGTT 
GAGAAAAGCT GCCGGGTCTT GATCATCGAT GACTCCCTCT ATGAAGAGGA GGAATCCTTC 
AGTGTTTCAC TGAGCCTGCC AGTGGGAGGG CGACTGGGAG CCCAGTTCCC CACCACCAAG 
GTGGTTATCC TGGCTGATCC TAATGATGAG CCTACCCTGC ACTTTGGGAA TGCTGAGTAT 
CATGTCGATG AAAGTGCTGG CTCCTTAGAG ATCTGTGTCT GGAGGACAGG CCCTGACCTT 
TCACAAGCAT CATCTGTCAC TGTGCACTCC AGGAAGACAG AGCCAGCATC AGCAGAAGCT 
GGAATAGATT ATGTTGGCAT CAGCCGAAAT CTGGGCTTTG CACCAGGAGT GCATATGCAG 
ATATTCCAAG TGACAATCCT TGATGACCTG GGACAACCCA TCTTGGAGGG TCCAGAGAGG 
TTTGAATTAG TTCTCCAGAT GCCAGTGGGT GCAGTGCTTG GAGAACCAAA TAAAACCACC 
ATTTTCATCA ATGACACCAT CACTGACTGT AAGCAGAGTA CTTGTAGTTC CTTTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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FREM3 cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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