ARFGEF1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARFGEF1
Entrez Gene ID	420170
Full Name	ADP ribosylation factor guanine nucleotide exchange factor 1
General protein information	Preferred Names ADP ribosylation factor guanine nucleotide exchange factor 1 Names brefeldin A-inhibited guanine nucleotide-exchange protein 1 ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_418283.5	XP_418283.3	brefeldin A-inhibited guanine nucleotide-exchange protein 1
XM_418283.6	XP_418283.3	brefeldin A-inhibited guanine nucleotide-exchange protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Neurospora crassa	NCU07658	XP_962785.2
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1

Gene Ontology

The following ARFGEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARFGEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa146435	XM_418283.6 Latest version!	Gallus gallus ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa05306	XM_418283.5 Latest version!	Gallus gallus ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (ARFGEF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARFGEF1 ( XM_418283.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_418283.3 Gallus gallus ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.

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CloneID	OGa146435
Clone ID Related Accession (Same CDS sequence)	XM_418283.6
Accession Version	XM_418283.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_418283.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG GGAAGAAGAC GAAAAACATG TTCTTGACCC GGGCCCTGGA GAAGATCCTG 
GCCGACAAGG AGGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCC 
CTCGAGGAAA TAAAGACTGA AACAGAGAAG CAAAGTCCCC CTCATGGAGA AGCAAAACCT 
GGTTCCAGCA CTCTGCCACC TGTGAAATCA AAGACGAGCT TCATTGAAGC AGACAAGTAC 
TTCCTACCAT TTGAATTGGC ATGCCAGTCA AAATGCCCCA GGATTGTCAG TACGTCTCTA 
GATTGTTTAC AGAAACTTAT TGCGTATGGT CATCTGACAG GGAATGCCCC AGACAGTACA 
GCTCCAGGCA AAAAATTAAT TGATAGAATT ATTGAGACAA TATGTGGCTG TTTTCAAGGA 
CCTCAAACAG ATGAAGGAGT TCAGCTACAA ATAATAAAGG CTTTACTTAC TGCAGTGACA 
TCTCAACATA TAGAAATCCA CGAAGGAACA GTGCTGCAGG CTGTTAGAAC ATGCTACAAT 
ATCTATCTAG CAAGCAAAAA TCTCATAAAT CAAACCACAG CCAAGGCCAC TCTAACACAG 
ATGCTGAATG TCATATTTGC ACGTATGGAA AACCAAGCAC TTCAGGAAGC CAAACAGATG 
GAAAAAGAAA GACACAGACA GCAGCACCAT TTACAGCAGT CTCCAGTAAG CCAGCATGAG 
CCTGAGTCAC CTCAGTTGAG ACATATTCCA ACACAGGCTG ATCAGCTGTC TAAAGAACAT 
GAGAGAGAGC TTGACCACAA TACGAATGAT GTGGACAAGA ACCTTCAAGA AGATATAGAG 
ACAGAAAATG GATCTGATAT TTCTAGTGCT GAAAATGAGC AGACAGAAGC TGACCAAGCC 
ACAGCAGCAG AAAATCTTTC TAAAGCTGAT GGAGAAACGT ATGATGATGA AAGCAATGAA 
TGTGAAGAGA AGGCACAAGA AATAGTAGAA AGTATTGTGC AGGAAATGGT GAACATAGTA 
GTTGGAGATA TAGAAGGATC TGCTGAAAGT GCAAGTGGTG ATGGAACAAT TGTAACATTA 
GAGGATGGCA GTGACAGTGA AAACATTCAG GCAAATGGAA TTCCAGGGAC TCCAATTTCT 
GTTGCTTATA CACCATCTTT ACCGGATGAC AGATTATCCG TCTCTTCCAA TGATACTCAG 
GAATCTGGAA ATTCTTCAGG ACCTACACCT GGTGCTAAGT TTTCCCACAT TTTACAAAAG 
GATGCCTTCC TTGTATTCAG GTCACTGTGT AAACTCTCCA TGAAGCCCCT ATCAGATGGA 
CCACCAGATC CAAAATCTCA TGAACTGCGG TCAAAGATTC TTTCATTGCA GTTGCTTCTG 
TCCATTCTAC AAAATGCAGG ACCTGTCTTC AGGACAAATG AGATGTTTAT TAATGCTATT 
AAGCAGTATC TTTGTGTTGC ACTGTCAAAA AATGGAGTCT CGTCAGTTCC AGAAGTTTTT 
GAACTTTCAC TTTCCATATT TCTTACCCTT CTCTCAAACT TTAAGACTCA TCTGAAGATG 
CAGATAGAGG TTTTCTTCAA AGAAATTTTC CTCTATATCT TGGAAACATC TACTAGCTCA 
TTTGATCATA AGTGGATGGT TATACAGACG CTAACACGGA TATGTGCAGA TGCCCAGAGT 
GTAGTGGACA TCTACGTGAA CTATGATTGT GATTTAAATG CAGCAAATAT ATTTGAAAGG 
CTGGTTAATG ACCTATCAAA GATAGCTCAA GGAAGAGGTA GCCAAGAACT TGGCATGTCC 
AACATTCAGG AATTAAGTTT GAGGAAGAAA GGATTGGAGT GCTTAGTATC AATCTTGAAA 
TGCATGGTGG AGTGGAGTAA GGATCAGTAT GTAAATCCCA ATTCTCAGAC AACACTTGGG 
CAAGAAAAAC CTACAGAACA GGACAGCAAT GAAACCAAAC ACCCTGAGAC AATTAACAGA 
TATGGAAGCT TAAATTCCCT GGATTCTACT GCTTCATCAG GAATTGGCAG CTACAGCACA 
CAGATGTCTG GCACTGACAA CCCGGAGCAG TTTGAGGTCC TAAAGCAACA AAAGGAAATA 
ATAGAACAAG GAATTGACTT ATTCAATAAG AAGCCAAAGA GGGGGATTCA GTACCTGCAA 
GAGCAAGGAA TGCTTGGCAC TACCCCTGAA GATATTGCTC AGTTCTTGCA CCAGGAGGAG 
AGATTAGATT CAACTCAGGT TGGGGAGTTC CTGGGAGACA ATGATAAATT CAACAAAGAA 
GTCATGTATG CATACGTAGA CCAACACGAC TTCTCAGGAA AGGATTTTGT ATCAGCTCTA 
CGTCTGTTTC TGGAGGGATT TCGCCTTCCA GGAGAGGCTC AAAAAATTGA TCGGCTAATG 
GAGAAATTTG CTGCTAGATA TTTAGAATGT AACCAAGGGC AAACTCTTTT TGCTAGTGCA 
GACACTGCGT ATGTCTTAGC TTACTCAATT ATCATGTTGA CAACAGATCT TCACAGTCCA 
CAGGTTAAGA ATAAAATGAC AAAAGAACAA TATATTAAAA TGAATAGAGG TATCAATGAC 
AGTAAAGATC TCCCTGAAGA ATATCTGTCT GCTATCTATA ATGAAATAGC TGGAAAGAAG 
ATTTCAATGA AAGAAACAAA AGAATTAACA ATACCTACAA AATCCAGTAA GCAAAGTGTT 
GCCAGTGAAA AGCAGAGAAG GCTTCTGTAT AATCTGGAAA TGGAACAAAT GGCTAAGACA 
GCTAAAGCTC TTATGGAGGC AGTGAGTCAT GTTCAGGCAC CATTTACCAG TGCAACACAC 
CTGGAGCATG TGAGACCCAT GTTTAAGTTG GCATGGACAC CTTTCCTAGC TGCCTTCAGT 
GTGGGTCTAC AGGACTGTGA TGACACAGAA GTTGCATCTC TTTGTTTGGA GGGCATACGG 
TGTGCCATCA GGATTGCCTG CATTTTCAAT ATACAGCTTG AAAGAGATGC CTACGTCCAA 
GCATTAGCAA GATTTACATT GCTTACAGTG AGTTCTGGTA TTACTGAAAT GAAGCAGAAG 
AATATTGACA CAATTAAAAC TCTCATCACA GTGGCTCATA CAGATGGAAA CTATTTAGGA 
AATTCCTGGC ATGAGATTCT GAAATGCATC AGTCAACTTG AGCTGGCACA GCTAATAGGA 
ACTGGAGTAA AACCTCGTTA CATCTCAGGG ACAGTGCGTG GCAGGGAAGG TTCTTTTACA 
GGAACAAAAG ATCAGGCACC TGATGAATTT GTGGGGCTAG GGCTGGTTGG TGGAAACGTG 
GATTGGAAGC AGATAGCAAG TATTCAAGAG TCCATTGGTG AAACTAGCTC CCAAAGTGTT 
GTCGTTGCTG TAGACAGAAT ATTTACAGGA TCTACAAGAT TGGATGGAAA TGCTATTGTG 
GATTTCGTTC GTTGGCTTTG TGCTGTATCT ATGGATGAGC TGCTGTCTGC TACTCACCCT 
CGAATGTTCA GCCTGCAGAA GATAGTAGAG ATTTCTTACT ACAACATGGG TCGGATTAGG 
CTACAGTGGT CTAGAATCTG GGAAGTTATT GGAGATCATT TTAATAAGGT TGGCTGCAAT 
CCCAATGAAG ATGTAGCTAT TTTTGCGGTA GACTCATTAA GGCAGTTATC CATGAAGTTC 
CTAGAAAAAG GAGAACTTGC TAATTTCCGA TTTCAGAAGG ACTTCTTAAG ACCGTTTGAA 
CATATAATGA AGAGAAACAG GTCTCCTACT ATTCGAGACA TGGTTGTGCG GTGCATTGCG 
CAGATGGTGA ATTCCCAAGC TGCCAACATC CGTTCTGGTT GGAAGAATAT TTTTTCAGTT 
TTTCATTTGG CTGCCTCAGA TCAGGATGAA AGTATAGTGG AACTTGCATT CCAGACTACA 
GGGCACATTG TCACGATTGT GTTTGAAAAA CACTTCCCAG CAACCATAGA TTCTTTCCAG 
GATGCAGTAA AGTGCTTGTC TGAATTTGCC TGCAATGCAG CATTTCCAGA TACCAGTATG 
GAAGCCATCC GCCTCATTCG CCACTGTGCA AAATACGTAT CTGATAGACC TCAGGCCTTC 
AAGGAATATA CGAGTGATGA TATGAATGTA GCTCCTGAAG ACAGAGTGTG GGTGAGAGGA 
TGGTTCCCAA TTCTGTTTGA GTTATCCTGT ATCATCAATA GATGCAAATT AGATGTAAGA 
ACCAGGGGCT TAACAGTAAT GTTTGAGATA ATGAAGACAT ATGGCCATAC GTATGAAAAA 
CACTGGTGGC AAGATTTATT TCGTATTGTC TTCAGGATAT TTGACAACAT GAAACTGCCA 
GAACAGCAGA CTGAGAAAGC TGAATGGATG ACGACTACTT GCAACCATGC ACTTTATGCA 
ATATGTGATG TATTCACACA ATATCTGGAA GTACTTAGTG ATGTTCTCCT GGATGACATA 
TTTGCACAGC TCTATTGGTG TGTGCAGCAA GACAATGAAC AACTGGCACG GTCTGGTACA 
AACTGTTTGG AGAATGTTGT TATCCTAAAT GGCGAGAAGT TTACCCTAGA AATCTGGGAT 
AAAACCTGCA CTTGCATGCT GGACATCTTC AAAACTACAA TTCCTCATGC GCTGCTGACT 
TGGAAGCCGG TCAGTGGGGG CTTTGGCCCT GTATCACCCT CTGATGCAAA AGAAAAACTG 
GATACAATCT CCCAGAAGTC TGTAGATATT CATGATACTG TTCAACCGAG ATCTTCAGAT 
GGACGGCCAC ATCAACCCAG TGCAGTGCCT ACAGGAAGTG AAGAAGTGAG CAAAGCCAGA 
TCAACAGCAA AATTTCCAGA ACAGAAGTTA TTTGCTGCTC TTCTGATCAA GTGTGTTGTA 
CAACTGGAAC TCATTCAGAC TATCGACAAC ATCGTGTTCT TCCCTGCAAC TAGCAAGAAA 
GAGGATGCAG AGAACCTAGC AGCAGCACAA AGAGATGCAG TAGATTTTGA TGTCCACGTG 
GATACCCAAG ATCAAGGGAT GTATCGTTTT CTGACATCAC AGCAGCTTTT CAAACTCCTG 
GATTGTCTGC TAGAATCTCA CAGGTTTGCT AAGGCTTTTA ATTCCAACAA TGAACAAAGA 
ACTGCTCTCT GGAAAGCAGG TTTTAAAGGC AAATCCAAGC CCAATCTTCT GAAGCAGGAG 
ACAAGCAGTC TTGCCTGTGG GTTACGCATT CTGTTCCGCA TGTACATGGA TGAAAGCCGA 
ATCAGTGCGT GGGAAGAAGT CCAGCAAAGG CTGCTAAATG TCTGCAGTGA GGCTCTGAGT 
TACTTTCTTA CTCTCACGTC AGAAAGCCAT CGGGAGGCCT GGACTAATCT ACTGCTCTTA 
TTTTTGACTA AAGTTCTGAA GATAAGTGAT GAAAGGTTCA AGGCTCACGC CTCTTTCTAC 
TATCCTCTCT TGTGTGAAAT TATGCAGTTT GACCTTATCC CTGAACTCCG AGCTGTTCTG 
CGAAGGTTCT TCCTGCGGAT CGGCGTGGTT TTTCAGATAT CCCAACCACC GGAGCACGAG 
TCTGCAATAA GCAAGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_418283.3
CDS	190..5730
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKTETEKQSPPHGEAKPGSSTLPPVKSKTSFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTAPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQQHHLQQSPVSQHEPESPQLRHIPTQADQLSKEHERELDHNTNDVDKNLQEDIETENGSDISSAENEQTEADQATAAENLSKADGETYDDESNECEEKAQEIVESIVQEMVNIVVGDIEGSAESASGDGTIVTLEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPTPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNIQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPTEQDSNETKHPETINRYGSLNSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRLFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQSVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFNIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSFTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSATHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTIVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCTCMLDIFKTTIPHALLTWKPVSGGFGPVSPSDAKEKLDTISQKSVDIHDTVQPRSSDGRPHQPSAVPTGSEEVSKARSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVHVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRISAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDERFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEHESAISKQ

Target ORF information:

RefSeq Version	XM_418283.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_418283.6

ORF Insert Sequence:

ATGTATGAAG GGAAGAAGAC GAAAAACATG TTCTTGACCC GGGCCCTGGA GAAGATCCTG 
GCCGACAAGG AGGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCC 
CTCGAGGAAA TAAAGACTGA AACAGAGAAG CAAAGTCCCC CTCATGGAGA AGCAAAACCT 
GGTTCCAGCA CTCTGCCACC TGTGAAATCA AAGACGAGCT TCATTGAAGC AGACAAGTAC 
TTCCTACCAT TTGAATTGGC ATGCCAGTCA AAATGCCCCA GGATTGTCAG TACGTCTCTA 
GATTGTTTAC AGAAACTTAT TGCGTATGGT CATCTGACAG GGAATGCCCC AGACAGTACA 
GCTCCAGGCA AAAAATTAAT TGATAGAATT ATTGAGACAA TATGTGGCTG TTTTCAAGGA 
CCTCAAACAG ATGAAGGAGT TCAGCTACAA ATAATAAAGG CTTTACTTAC TGCAGTGACA 
TCTCAACATA TAGAAATCCA CGAAGGAACA GTGCTGCAGG CTGTTAGAAC ATGCTACAAT 
ATCTATCTAG CAAGCAAAAA TCTCATAAAT CAAACCACAG CCAAGGCCAC TCTAACACAG 
ATGCTGAATG TCATATTTGC ACGTATGGAA AACCAAGCAC TTCAGGAAGC CAAACAGATG 
GAAAAAGAAA GACACAGACA GCAGCACCAT TTACAGCAGT CTCCAGTAAG CCAGCATGAG 
CCTGAGTCAC CTCAGTTGAG ACATATTCCA ACACAGGCTG ATCAGCTGTC TAAAGAACAT 
GAGAGAGAGC TTGACCACAA TACGAATGAT GTGGACAAGA ACCTTCAAGA AGATATAGAG 
ACAGAAAATG GATCTGATAT TTCTAGTGCT GAAAATGAGC AGACAGAAGC TGACCAAGCC 
ACAGCAGCAG AAAATCTTTC TAAAGCTGAT GGAGAAACGT ATGATGATGA AAGCAATGAA 
TGTGAAGAGA AGGCACAAGA AATAGTAGAA AGTATTGTGC AGGAAATGGT GAACATAGTA 
GTTGGAGATA TAGAAGGATC TGCTGAAAGT GCAAGTGGTG ATGGAACAAT TGTAACATTA 
GAGGATGGCA GTGACAGTGA AAACATTCAG GCAAATGGAA TTCCAGGGAC TCCAATTTCT 
GTTGCTTATA CACCATCTTT ACCGGATGAC AGATTATCCG TCTCTTCCAA TGATACTCAG 
GAATCTGGAA ATTCTTCAGG ACCTACACCT GGTGCTAAGT TTTCCCACAT TTTACAAAAG 
GATGCCTTCC TTGTATTCAG GTCACTGTGT AAACTCTCCA TGAAGCCCCT ATCAGATGGA 
CCACCAGATC CAAAATCTCA TGAACTGCGG TCAAAGATTC TTTCATTGCA GTTGCTTCTG 
TCCATTCTAC AAAATGCAGG ACCTGTCTTC AGGACAAATG AGATGTTTAT TAATGCTATT 
AAGCAGTATC TTTGTGTTGC ACTGTCAAAA AATGGAGTCT CGTCAGTTCC AGAAGTTTTT 
GAACTTTCAC TTTCCATATT TCTTACCCTT CTCTCAAACT TTAAGACTCA TCTGAAGATG 
CAGATAGAGG TTTTCTTCAA AGAAATTTTC CTCTATATCT TGGAAACATC TACTAGCTCA 
TTTGATCATA AGTGGATGGT TATACAGACG CTAACACGGA TATGTGCAGA TGCCCAGAGT 
GTAGTGGACA TCTACGTGAA CTATGATTGT GATTTAAATG CAGCAAATAT ATTTGAAAGG 
CTGGTTAATG ACCTATCAAA GATAGCTCAA GGAAGAGGTA GCCAAGAACT TGGCATGTCC 
AACATTCAGG AATTAAGTTT GAGGAAGAAA GGATTGGAGT GCTTAGTATC AATCTTGAAA 
TGCATGGTGG AGTGGAGTAA GGATCAGTAT GTAAATCCCA ATTCTCAGAC AACACTTGGG 
CAAGAAAAAC CTACAGAACA GGACAGCAAT GAAACCAAAC ACCCTGAGAC AATTAACAGA 
TATGGAAGCT TAAATTCCCT GGATTCTACT GCTTCATCAG GAATTGGCAG CTACAGCACA 
CAGATGTCTG GCACTGACAA CCCGGAGCAG TTTGAGGTCC TAAAGCAACA AAAGGAAATA 
ATAGAACAAG GAATTGACTT ATTCAATAAG AAGCCAAAGA GGGGGATTCA GTACCTGCAA 
GAGCAAGGAA TGCTTGGCAC TACCCCTGAA GATATTGCTC AGTTCTTGCA CCAGGAGGAG 
AGATTAGATT CAACTCAGGT TGGGGAGTTC CTGGGAGACA ATGATAAATT CAACAAAGAA 
GTCATGTATG CATACGTAGA CCAACACGAC TTCTCAGGAA AGGATTTTGT ATCAGCTCTA 
CGTCTGTTTC TGGAGGGATT TCGCCTTCCA GGAGAGGCTC AAAAAATTGA TCGGCTAATG 
GAGAAATTTG CTGCTAGATA TTTAGAATGT AACCAAGGGC AAACTCTTTT TGCTAGTGCA 
GACACTGCGT ATGTCTTAGC TTACTCAATT ATCATGTTGA CAACAGATCT TCACAGTCCA 
CAGGTTAAGA ATAAAATGAC AAAAGAACAA TATATTAAAA TGAATAGAGG TATCAATGAC 
AGTAAAGATC TCCCTGAAGA ATATCTGTCT GCTATCTATA ATGAAATAGC TGGAAAGAAG 
ATTTCAATGA AAGAAACAAA AGAATTAACA ATACCTACAA AATCCAGTAA GCAAAGTGTT 
GCCAGTGAAA AGCAGAGAAG GCTTCTGTAT AATCTGGAAA TGGAACAAAT GGCTAAGACA 
GCTAAAGCTC TTATGGAGGC AGTGAGTCAT GTTCAGGCAC CATTTACCAG TGCAACACAC 
CTGGAGCATG TGAGACCCAT GTTTAAGTTG GCATGGACAC CTTTCCTAGC TGCCTTCAGT 
GTGGGTCTAC AGGACTGTGA TGACACAGAA GTTGCATCTC TTTGTTTGGA GGGCATACGG 
TGTGCCATCA GGATTGCCTG CATTTTCAAT ATACAGCTTG AAAGAGATGC CTACGTCCAA 
GCATTAGCAA GATTTACATT GCTTACAGTG AGTTCTGGTA TTACTGAAAT GAAGCAGAAG 
AATATTGACA CAATTAAAAC TCTCATCACA GTGGCTCATA CAGATGGAAA CTATTTAGGA 
AATTCCTGGC ATGAGATTCT GAAATGCATC AGTCAACTTG AGCTGGCACA GCTAATAGGA 
ACTGGAGTAA AACCTCGTTA CATCTCAGGG ACAGTGCGTG GCAGGGAAGG TTCTTTTACA 
GGAACAAAAG ATCAGGCACC TGATGAATTT GTGGGGCTAG GGCTGGTTGG TGGAAACGTG 
GATTGGAAGC AGATAGCAAG TATTCAAGAG TCCATTGGTG AAACTAGCTC CCAAAGTGTT 
GTCGTTGCTG TAGACAGAAT ATTTACAGGA TCTACAAGAT TGGATGGAAA TGCTATTGTG 
GATTTCGTTC GTTGGCTTTG TGCTGTATCT ATGGATGAGC TGCTGTCTGC TACTCACCCT 
CGAATGTTCA GCCTGCAGAA GATAGTAGAG ATTTCTTACT ACAACATGGG TCGGATTAGG 
CTACAGTGGT CTAGAATCTG GGAAGTTATT GGAGATCATT TTAATAAGGT TGGCTGCAAT 
CCCAATGAAG ATGTAGCTAT TTTTGCGGTA GACTCATTAA GGCAGTTATC CATGAAGTTC 
CTAGAAAAAG GAGAACTTGC TAATTTCCGA TTTCAGAAGG ACTTCTTAAG ACCGTTTGAA 
CATATAATGA AGAGAAACAG GTCTCCTACT ATTCGAGACA TGGTTGTGCG GTGCATTGCG 
CAGATGGTGA ATTCCCAAGC TGCCAACATC CGTTCTGGTT GGAAGAATAT TTTTTCAGTT 
TTTCATTTGG CTGCCTCAGA TCAGGATGAA AGTATAGTGG AACTTGCATT CCAGACTACA 
GGGCACATTG TCACGATTGT GTTTGAAAAA CACTTCCCAG CAACCATAGA TTCTTTCCAG 
GATGCAGTAA AGTGCTTGTC TGAATTTGCC TGCAATGCAG CATTTCCAGA TACCAGTATG 
GAAGCCATCC GCCTCATTCG CCACTGTGCA AAATACGTAT CTGATAGACC TCAGGCCTTC 
AAGGAATATA CGAGTGATGA TATGAATGTA GCTCCTGAAG ACAGAGTGTG GGTGAGAGGA 
TGGTTCCCAA TTCTGTTTGA GTTATCCTGT ATCATCAATA GATGCAAATT AGATGTAAGA 
ACCAGGGGCT TAACAGTAAT GTTTGAGATA ATGAAGACAT ATGGCCATAC GTATGAAAAA 
CACTGGTGGC AAGATTTATT TCGTATTGTC TTCAGGATAT TTGACAACAT GAAACTGCCA 
GAACAGCAGA CTGAGAAAGC TGAATGGATG ACGACTACTT GCAACCATGC ACTTTATGCA 
ATATGTGATG TATTCACACA ATATCTGGAA GTACTTAGTG ATGTTCTCCT GGATGACATA 
TTTGCACAGC TCTATTGGTG TGTGCAGCAA GACAATGAAC AACTGGCACG GTCTGGTACA 
AACTGTTTGG AGAATGTTGT TATCCTAAAT GGCGAGAAGT TTACCCTAGA AATCTGGGAT 
AAAACCTGCA CTTGCATGCT GGACATCTTC AAAACTACAA TTCCTCATGC GCTGCTGACT 
TGGAAGCCGG TCAGTGGGGG CTTTGGCCCT GTATCACCCT CTGATGCAAA AGAAAAACTG 
GATACAATCT CCCAGAAGTC TGTAGATATT CATGATACTG TTCAACCGAG ATCTTCAGAT 
GGACGGCCAC ATCAACCCAG TGCAGTGCCT ACAGGAAGTG AAGAAGTGAG CAAAGCCAGA 
TCAACAGCAA AATTTCCAGA ACAGAAGTTA TTTGCTGCTC TTCTGATCAA GTGTGTTGTA 
CAACTGGAAC TCATTCAGAC TATCGACAAC ATCGTGTTCT TCCCTGCAAC TAGCAAGAAA 
GAGGATGCAG AGAACCTAGC AGCAGCACAA AGAGATGCAG TAGATTTTGA TGTCCACGTG 
GATACCCAAG ATCAAGGGAT GTATCGTTTT CTGACATCAC AGCAGCTTTT CAAACTCCTG 
GATTGTCTGC TAGAATCTCA CAGGTTTGCT AAGGCTTTTA ATTCCAACAA TGAACAAAGA 
ACTGCTCTCT GGAAAGCAGG TTTTAAAGGC AAATCCAAGC CCAATCTTCT GAAGCAGGAG 
ACAAGCAGTC TTGCCTGTGG GTTACGCATT CTGTTCCGCA TGTACATGGA TGAAAGCCGA 
ATCAGTGCGT GGGAAGAAGT CCAGCAAAGG CTGCTAAATG TCTGCAGTGA GGCTCTGAGT 
TACTTTCTTA CTCTCACGTC AGAAAGCCAT CGGGAGGCCT GGACTAATCT ACTGCTCTTA 
TTTTTGACTA AAGTTCTGAA GATAAGTGAT GAAAGGTTCA AGGCTCACGC CTCTTTCTAC 
TATCCTCTCT TGTGTGAAAT TATGCAGTTT GACCTTATCC CTGAACTCCG AGCTGTTCTG 
CGAAGGTTCT TCCTGCGGAT CGGCGTGGTT TTTCAGATAT CCCAACCACC GGAGCACGAG 
TCTGCAATAA GCAAGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( XM_418283.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_418283.3 Gallus gallus ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (ARFGEF1), mRNA.

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CloneID	OGa05306
Clone ID Related Accession (Same CDS sequence)	XM_418283.5
Accession Version	XM_418283.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455809.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_418283.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG GGAAGAAGAC GAAAAACATG TTCTTGACCC GGGCCCTGGA GAAGATCCTG 
GCCGACAAGG AGGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCC 
CTCGAGGAAA TAAAGACTGA AACAGAGAAG CAAAGTCCCC CTCATGGAGA AGCAAAACCT 
GGTTCCAGCA CTCTGCCACC TGTGAAATCA AAGACGAGCT TCATTGAAGC AGACAAGTAC 
TTCCTACCAT TTGAATTGGC ATGCCAGTCA AAATGCCCCA GGATTGTCAG TACGTCTCTA 
GATTGTTTAC AGAAACTTAT TGCGTATGGT CATCTGACAG GGAATGCCCC AGACAGTACA 
GCTCCAGGCA AAAAATTAAT TGATAGAATT ATTGAGACAA TATGTGGCTG TTTTCAAGGA 
CCTCAAACAG ATGAAGGAGT TCAGCTACAA ATAATAAAGG CTTTACTTAC TGCAGTGACA 
TCTCAACATA TAGAAATCCA CGAAGGAACA GTGCTGCAGG CTGTTAGAAC ATGCTACAAT 
ATCTATCTAG CAAGCAAAAA TCTCATAAAT CAAACCACAG CCAAGGCCAC TCTAACACAG 
ATGCTGAATG TCATATTTGC ACGTATGGAA AACCAAGCAC TTCAGGAAGC CAAACAGATG 
GAAAAAGAAA GACACAGACA GCAGCACCAT TTACAGCAGT CTCCAGTAAG CCAGCATGAG 
CCTGAGTCAC CTCAGTTGAG ACATATTCCA ACACAGGCTG ATCAGCTGTC TAAAGAACAT 
GAGAGAGAGC TTGACCACAA TACGAATGAT GTGGACAAGA ACCTTCAAGA AGATATAGAG 
ACAGAAAATG GATCTGATAT TTCTAGTGCT GAAAATGAGC AGACAGAAGC TGACCAAGCC 
ACAGCAGCAG AAAATCTTTC TAAAGCTGAT GGAGAAACGT ATGATGATGA AAGCAATGAA 
TGTGAAGAGA AGGCACAAGA AATAGTAGAA AGTATTGTGC AGGAAATGGT GAACATAGTA 
GTTGGAGATA TAGAAGGATC TGCTGAAAGT GCAAGTGGTG ATGGAACAAT TGTAACATTA 
GAGGATGGCA GTGACAGTGA AAACATTCAG GCAAATGGAA TTCCAGGGAC TCCAATTTCT 
GTTGCTTATA CACCATCTTT ACCGGATGAC AGATTATCCG TCTCTTCCAA TGATACTCAG 
GAATCTGGAA ATTCTTCAGG ACCTACACCT GGTGCTAAGT TTTCCCACAT TTTACAAAAG 
GATGCCTTCC TTGTATTCAG GTCACTGTGT AAACTCTCCA TGAAGCCCCT ATCAGATGGA 
CCACCAGATC CAAAATCTCA TGAACTGCGG TCAAAGATTC TTTCATTGCA GTTGCTTCTG 
TCCATTCTAC AAAATGCAGG ACCTGTCTTC AGGACAAATG AGATGTTTAT TAATGCTATT 
AAGCAGTATC TTTGTGTTGC ACTGTCAAAA AATGGAGTCT CGTCAGTTCC AGAAGTTTTT 
GAACTTTCAC TTTCCATATT TCTTACCCTT CTCTCAAACT TTAAGACTCA TCTGAAGATG 
CAGATAGAGG TTTTCTTCAA AGAAATTTTC CTCTATATCT TGGAAACATC TACTAGCTCA 
TTTGATCATA AGTGGATGGT TATACAGACG CTAACACGGA TATGTGCAGA TGCCCAGAGT 
GTAGTGGACA TCTACGTGAA CTATGATTGT GATTTAAATG CAGCAAATAT ATTTGAAAGG 
CTGGTTAATG ACCTATCAAA GATAGCTCAA GGAAGAGGTA GCCAAGAACT TGGCATGTCC 
AACATTCAGG AATTAAGTTT GAGGAAGAAA GGATTGGAGT GCTTAGTATC AATCTTGAAA 
TGCATGGTGG AGTGGAGTAA GGATCAGTAT GTAAATCCCA ATTCTCAGAC AACACTTGGG 
CAAGAAAAAC CTACAGAACA GGACAGCAAT GAAACCAAAC ACCCTGAGAC AATTAACAGA 
TATGGAAGCT TAAATTCCCT GGATTCTACT GCTTCATCAG GAATTGGCAG CTACAGCACA 
CAGATGTCTG GCACTGACAA CCCGGAGCAG TTTGAGGTCC TAAAGCAACA AAAGGAAATA 
ATAGAACAAG GAATTGACTT ATTCAATAAG AAGCCAAAGA GGGGGATTCA GTACCTGCAA 
GAGCAAGGAA TGCTTGGCAC TACCCCTGAA GATATTGCTC AGTTCTTGCA CCAGGAGGAG 
AGATTAGATT CAACTCAGGT TGGGGAGTTC CTGGGAGACA ATGATAAATT CAACAAAGAA 
GTCATGTATG CATACGTAGA CCAACACGAC TTCTCAGGAA AGGATTTTGT ATCAGCTCTA 
CGTCTGTTTC TGGAGGGATT TCGCCTTCCA GGAGAGGCTC AAAAAATTGA TCGGCTAATG 
GAGAAATTTG CTGCTAGATA TTTAGAATGT AACCAAGGGC AAACTCTTTT TGCTAGTGCA 
GACACTGCGT ATGTCTTAGC TTACTCAATT ATCATGTTGA CAACAGATCT TCACAGTCCA 
CAGGTTAAGA ATAAAATGAC AAAAGAACAA TATATTAAAA TGAATAGAGG TATCAATGAC 
AGTAAAGATC TCCCTGAAGA ATATCTGTCT GCTATCTATA ATGAAATAGC TGGAAAGAAG 
ATTTCAATGA AAGAAACAAA AGAATTAACA ATACCTACAA AATCCAGTAA GCAAAGTGTT 
GCCAGTGAAA AGCAGAGAAG GCTTCTGTAT AATCTGGAAA TGGAACAAAT GGCTAAGACA 
GCTAAAGCTC TTATGGAGGC AGTGAGTCAT GTTCAGGCAC CATTTACCAG TGCAACACAC 
CTGGAGCATG TGAGACCCAT GTTTAAGTTG GCATGGACAC CTTTCCTAGC TGCCTTCAGT 
GTGGGTCTAC AGGACTGTGA TGACACAGAA GTTGCATCTC TTTGTTTGGA GGGCATACGG 
TGTGCCATCA GGATTGCCTG CATTTTCAAT ATACAGCTTG AAAGAGATGC CTACGTCCAA 
GCATTAGCAA GATTTACATT GCTTACAGTG AGTTCTGGTA TTACTGAAAT GAAGCAGAAG 
AATATTGACA CAATTAAAAC TCTCATCACA GTGGCTCATA CAGATGGAAA CTATTTAGGA 
AATTCCTGGC ATGAGATTCT GAAATGCATC AGTCAACTTG AGCTGGCACA GCTAATAGGA 
ACTGGAGTAA AACCTCGTTA CATCTCAGGG ACAGTGCGTG GCAGGGAAGG TTCTTTTACA 
GGAACAAAAG ATCAGGCACC TGATGAATTT GTGGGGCTAG GGCTGGTTGG TGGAAACGTG 
GATTGGAAGC AGATAGCAAG TATTCAAGAG TCCATTGGTG AAACTAGCTC CCAAAGTGTT 
GTCGTTGCTG TAGACAGAAT ATTTACAGGA TCTACAAGAT TGGATGGAAA TGCTATTGTG 
GATTTCGTTC GTTGGCTTTG TGCTGTATCT ATGGATGAGC TGCTGTCTGC TACTCACCCT 
CGAATGTTCA GCCTGCAGAA GATAGTAGAG ATTTCTTACT ACAACATGGG TCGGATTAGG 
CTACAGTGGT CTAGAATCTG GGAAGTTATT GGAGATCATT TTAATAAGGT TGGCTGCAAT 
CCCAATGAAG ATGTAGCTAT TTTTGCGGTA GACTCATTAA GGCAGTTATC CATGAAGTTC 
CTAGAAAAAG GAGAACTTGC TAATTTCCGA TTTCAGAAGG ACTTCTTAAG ACCGTTTGAA 
CATATAATGA AGAGAAACAG GTCTCCTACT ATTCGAGACA TGGTTGTGCG GTGCATTGCG 
CAGATGGTGA ATTCCCAAGC TGCCAACATC CGTTCTGGTT GGAAGAATAT TTTTTCAGTT 
TTTCATTTGG CTGCCTCAGA TCAGGATGAA AGTATAGTGG AACTTGCATT CCAGACTACA 
GGGCACATTG TCACGATTGT GTTTGAAAAA CACTTCCCAG CAACCATAGA TTCTTTCCAG 
GATGCAGTAA AGTGCTTGTC TGAATTTGCC TGCAATGCAG CATTTCCAGA TACCAGTATG 
GAAGCCATCC GCCTCATTCG CCACTGTGCA AAATACGTAT CTGATAGACC TCAGGCCTTC 
AAGGAATATA CGAGTGATGA TATGAATGTA GCTCCTGAAG ACAGAGTGTG GGTGAGAGGA 
TGGTTCCCAA TTCTGTTTGA GTTATCCTGT ATCATCAATA GATGCAAATT AGATGTAAGA 
ACCAGGGGCT TAACAGTAAT GTTTGAGATA ATGAAGACAT ATGGCCATAC GTATGAAAAA 
CACTGGTGGC AAGATTTATT TCGTATTGTC TTCAGGATAT TTGACAACAT GAAACTGCCA 
GAACAGCAGA CTGAGAAAGC TGAATGGATG ACGACTACTT GCAACCATGC ACTTTATGCA 
ATATGTGATG TATTCACACA ATATCTGGAA GTACTTAGTG ATGTTCTCCT GGATGACATA 
TTTGCACAGC TCTATTGGTG TGTGCAGCAA GACAATGAAC AACTGGCACG GTCTGGTACA 
AACTGTTTGG AGAATGTTGT TATCCTAAAT GGCGAGAAGT TTACCCTAGA AATCTGGGAT 
AAAACCTGCA CTTGCATGCT GGACATCTTC AAAACTACAA TTCCTCATGC GCTGCTGACT 
TGGAAGCCGG TCAGTGGGGG CTTTGGCCCT GTATCACCCT CTGATGCAAA AGAAAAACTG 
GATACAATCT CCCAGAAGTC TGTAGATATT CATGATACTG TTCAACCGAG ATCTTCAGAT 
GGACGGCCAC ATCAACCCAG TGCAGTGCCT ACAGGAAGTG AAGAAGTGAG CAAAGCCAGA 
TCAACAGCAA AATTTCCAGA ACAGAAGTTA TTTGCTGCTC TTCTGATCAA GTGTGTTGTA 
CAACTGGAAC TCATTCAGAC TATCGACAAC ATCGTGTTCT TCCCTGCAAC TAGCAAGAAA 
GAGGATGCAG AGAACCTAGC AGCAGCACAA AGAGATGCAG TAGATTTTGA TGTCCACGTG 
GATACCCAAG ATCAAGGGAT GTATCGTTTT CTGACATCAC AGCAGCTTTT CAAACTCCTG 
GATTGTCTGC TAGAATCTCA CAGGTTTGCT AAGGCTTTTA ATTCCAACAA TGAACAAAGA 
ACTGCTCTCT GGAAAGCAGG TTTTAAAGGC AAATCCAAGC CCAATCTTCT GAAGCAGGAG 
ACAAGCAGTC TTGCCTGTGG GTTACGCATT CTGTTCCGCA TGTACATGGA TGAAAGCCGA 
ATCAGTGCGT GGGAAGAAGT CCAGCAAAGG CTGCTAAATG TCTGCAGTGA GGCTCTGAGT 
TACTTTCTTA CTCTCACGTC AGAAAGCCAT CGGGAGGCCT GGACTAATCT ACTGCTCTTA 
TTTTTGACTA AAGTTCTGAA GATAAGTGAT GAAAGGTTCA AGGCTCACGC CTCTTTCTAC 
TATCCTCTCT TGTGTGAAAT TATGCAGTTT GACCTTATCC CTGAGCTCCG AGCTGTTCTG 
CGAAGGTTCT TCCTGCGGAT CGGCGTGGTT TTTCAGATAT CCCAACCACC GGAGCACGAG 
TCTGCAATAA GCAAGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_418283.3
CDS	232..5772
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKTETEKQSPPHGEAKPGSSTLPPVKSKTSFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTAPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQQHHLQQSPVSQHEPESPQLRHIPTQADQLSKEHERELDHNTNDVDKNLQEDIETENGSDISSAENEQTEADQATAAENLSKADGETYDDESNECEEKAQEIVESIVQEMVNIVVGDIEGSAESASGDGTIVTLEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPTPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNIQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPTEQDSNETKHPETINRYGSLNSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRLFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQSVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFNIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSFTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSATHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTIVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCTCMLDIFKTTIPHALLTWKPVSGGFGPVSPSDAKEKLDTISQKSVDIHDTVQPRSSDGRPHQPSAVPTGSEEVSKARSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVHVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRISAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDERFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEHESAISKQ

Target ORF information:

RefSeq Version	XM_418283.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (ARFGEF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_418283.5

ORF Insert Sequence:

ATGTATGAAG GGAAGAAGAC GAAAAACATG TTCTTGACCC GGGCCCTGGA GAAGATCCTG 
GCCGACAAGG AGGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCTTG CGAGGTGGCC 
CTCGAGGAAA TAAAGACTGA AACAGAGAAG CAAAGTCCCC CTCATGGAGA AGCAAAACCT 
GGTTCCAGCA CTCTGCCACC TGTGAAATCA AAGACGAGCT TCATTGAAGC AGACAAGTAC 
TTCCTACCAT TTGAATTGGC ATGCCAGTCA AAATGCCCCA GGATTGTCAG TACGTCTCTA 
GATTGTTTAC AGAAACTTAT TGCGTATGGT CATCTGACAG GGAATGCCCC AGACAGTACA 
GCTCCAGGCA AAAAATTAAT TGATAGAATT ATTGAGACAA TATGTGGCTG TTTTCAAGGA 
CCTCAAACAG ATGAAGGAGT TCAGCTACAA ATAATAAAGG CTTTACTTAC TGCAGTGACA 
TCTCAACATA TAGAAATCCA CGAAGGAACA GTGCTGCAGG CTGTTAGAAC ATGCTACAAT 
ATCTATCTAG CAAGCAAAAA TCTCATAAAT CAAACCACAG CCAAGGCCAC TCTAACACAG 
ATGCTGAATG TCATATTTGC ACGTATGGAA AACCAAGCAC TTCAGGAAGC CAAACAGATG 
GAAAAAGAAA GACACAGACA GCAGCACCAT TTACAGCAGT CTCCAGTAAG CCAGCATGAG 
CCTGAGTCAC CTCAGTTGAG ACATATTCCA ACACAGGCTG ATCAGCTGTC TAAAGAACAT 
GAGAGAGAGC TTGACCACAA TACGAATGAT GTGGACAAGA ACCTTCAAGA AGATATAGAG 
ACAGAAAATG GATCTGATAT TTCTAGTGCT GAAAATGAGC AGACAGAAGC TGACCAAGCC 
ACAGCAGCAG AAAATCTTTC TAAAGCTGAT GGAGAAACGT ATGATGATGA AAGCAATGAA 
TGTGAAGAGA AGGCACAAGA AATAGTAGAA AGTATTGTGC AGGAAATGGT GAACATAGTA 
GTTGGAGATA TAGAAGGATC TGCTGAAAGT GCAAGTGGTG ATGGAACAAT TGTAACATTA 
GAGGATGGCA GTGACAGTGA AAACATTCAG GCAAATGGAA TTCCAGGGAC TCCAATTTCT 
GTTGCTTATA CACCATCTTT ACCGGATGAC AGATTATCCG TCTCTTCCAA TGATACTCAG 
GAATCTGGAA ATTCTTCAGG ACCTACACCT GGTGCTAAGT TTTCCCACAT TTTACAAAAG 
GATGCCTTCC TTGTATTCAG GTCACTGTGT AAACTCTCCA TGAAGCCCCT ATCAGATGGA 
CCACCAGATC CAAAATCTCA TGAACTGCGG TCAAAGATTC TTTCATTGCA GTTGCTTCTG 
TCCATTCTAC AAAATGCAGG ACCTGTCTTC AGGACAAATG AGATGTTTAT TAATGCTATT 
AAGCAGTATC TTTGTGTTGC ACTGTCAAAA AATGGAGTCT CGTCAGTTCC AGAAGTTTTT 
GAACTTTCAC TTTCCATATT TCTTACCCTT CTCTCAAACT TTAAGACTCA TCTGAAGATG 
CAGATAGAGG TTTTCTTCAA AGAAATTTTC CTCTATATCT TGGAAACATC TACTAGCTCA 
TTTGATCATA AGTGGATGGT TATACAGACG CTAACACGGA TATGTGCAGA TGCCCAGAGT 
GTAGTGGACA TCTACGTGAA CTATGATTGT GATTTAAATG CAGCAAATAT ATTTGAAAGG 
CTGGTTAATG ACCTATCAAA GATAGCTCAA GGAAGAGGTA GCCAAGAACT TGGCATGTCC 
AACATTCAGG AATTAAGTTT GAGGAAGAAA GGATTGGAGT GCTTAGTATC AATCTTGAAA 
TGCATGGTGG AGTGGAGTAA GGATCAGTAT GTAAATCCCA ATTCTCAGAC AACACTTGGG 
CAAGAAAAAC CTACAGAACA GGACAGCAAT GAAACCAAAC ACCCTGAGAC AATTAACAGA 
TATGGAAGCT TAAATTCCCT GGATTCTACT GCTTCATCAG GAATTGGCAG CTACAGCACA 
CAGATGTCTG GCACTGACAA CCCGGAGCAG TTTGAGGTCC TAAAGCAACA AAAGGAAATA 
ATAGAACAAG GAATTGACTT ATTCAATAAG AAGCCAAAGA GGGGGATTCA GTACCTGCAA 
GAGCAAGGAA TGCTTGGCAC TACCCCTGAA GATATTGCTC AGTTCTTGCA CCAGGAGGAG 
AGATTAGATT CAACTCAGGT TGGGGAGTTC CTGGGAGACA ATGATAAATT CAACAAAGAA 
GTCATGTATG CATACGTAGA CCAACACGAC TTCTCAGGAA AGGATTTTGT ATCAGCTCTA 
CGTCTGTTTC TGGAGGGATT TCGCCTTCCA GGAGAGGCTC AAAAAATTGA TCGGCTAATG 
GAGAAATTTG CTGCTAGATA TTTAGAATGT AACCAAGGGC AAACTCTTTT TGCTAGTGCA 
GACACTGCGT ATGTCTTAGC TTACTCAATT ATCATGTTGA CAACAGATCT TCACAGTCCA 
CAGGTTAAGA ATAAAATGAC AAAAGAACAA TATATTAAAA TGAATAGAGG TATCAATGAC 
AGTAAAGATC TCCCTGAAGA ATATCTGTCT GCTATCTATA ATGAAATAGC TGGAAAGAAG 
ATTTCAATGA AAGAAACAAA AGAATTAACA ATACCTACAA AATCCAGTAA GCAAAGTGTT 
GCCAGTGAAA AGCAGAGAAG GCTTCTGTAT AATCTGGAAA TGGAACAAAT GGCTAAGACA 
GCTAAAGCTC TTATGGAGGC AGTGAGTCAT GTTCAGGCAC CATTTACCAG TGCAACACAC 
CTGGAGCATG TGAGACCCAT GTTTAAGTTG GCATGGACAC CTTTCCTAGC TGCCTTCAGT 
GTGGGTCTAC AGGACTGTGA TGACACAGAA GTTGCATCTC TTTGTTTGGA GGGCATACGG 
TGTGCCATCA GGATTGCCTG CATTTTCAAT ATACAGCTTG AAAGAGATGC CTACGTCCAA 
GCATTAGCAA GATTTACATT GCTTACAGTG AGTTCTGGTA TTACTGAAAT GAAGCAGAAG 
AATATTGACA CAATTAAAAC TCTCATCACA GTGGCTCATA CAGATGGAAA CTATTTAGGA 
AATTCCTGGC ATGAGATTCT GAAATGCATC AGTCAACTTG AGCTGGCACA GCTAATAGGA 
ACTGGAGTAA AACCTCGTTA CATCTCAGGG ACAGTGCGTG GCAGGGAAGG TTCTTTTACA 
GGAACAAAAG ATCAGGCACC TGATGAATTT GTGGGGCTAG GGCTGGTTGG TGGAAACGTG 
GATTGGAAGC AGATAGCAAG TATTCAAGAG TCCATTGGTG AAACTAGCTC CCAAAGTGTT 
GTCGTTGCTG TAGACAGAAT ATTTACAGGA TCTACAAGAT TGGATGGAAA TGCTATTGTG 
GATTTCGTTC GTTGGCTTTG TGCTGTATCT ATGGATGAGC TGCTGTCTGC TACTCACCCT 
CGAATGTTCA GCCTGCAGAA GATAGTAGAG ATTTCTTACT ACAACATGGG TCGGATTAGG 
CTACAGTGGT CTAGAATCTG GGAAGTTATT GGAGATCATT TTAATAAGGT TGGCTGCAAT 
CCCAATGAAG ATGTAGCTAT TTTTGCGGTA GACTCATTAA GGCAGTTATC CATGAAGTTC 
CTAGAAAAAG GAGAACTTGC TAATTTCCGA TTTCAGAAGG ACTTCTTAAG ACCGTTTGAA 
CATATAATGA AGAGAAACAG GTCTCCTACT ATTCGAGACA TGGTTGTGCG GTGCATTGCG 
CAGATGGTGA ATTCCCAAGC TGCCAACATC CGTTCTGGTT GGAAGAATAT TTTTTCAGTT 
TTTCATTTGG CTGCCTCAGA TCAGGATGAA AGTATAGTGG AACTTGCATT CCAGACTACA 
GGGCACATTG TCACGATTGT GTTTGAAAAA CACTTCCCAG CAACCATAGA TTCTTTCCAG 
GATGCAGTAA AGTGCTTGTC TGAATTTGCC TGCAATGCAG CATTTCCAGA TACCAGTATG 
GAAGCCATCC GCCTCATTCG CCACTGTGCA AAATACGTAT CTGATAGACC TCAGGCCTTC 
AAGGAATATA CGAGTGATGA TATGAATGTA GCTCCTGAAG ACAGAGTGTG GGTGAGAGGA 
TGGTTCCCAA TTCTGTTTGA GTTATCCTGT ATCATCAATA GATGCAAATT AGATGTAAGA 
ACCAGGGGCT TAACAGTAAT GTTTGAGATA ATGAAGACAT ATGGCCATAC GTATGAAAAA 
CACTGGTGGC AAGATTTATT TCGTATTGTC TTCAGGATAT TTGACAACAT GAAACTGCCA 
GAACAGCAGA CTGAGAAAGC TGAATGGATG ACGACTACTT GCAACCATGC ACTTTATGCA 
ATATGTGATG TATTCACACA ATATCTGGAA GTACTTAGTG ATGTTCTCCT GGATGACATA 
TTTGCACAGC TCTATTGGTG TGTGCAGCAA GACAATGAAC AACTGGCACG GTCTGGTACA 
AACTGTTTGG AGAATGTTGT TATCCTAAAT GGCGAGAAGT TTACCCTAGA AATCTGGGAT 
AAAACCTGCA CTTGCATGCT GGACATCTTC AAAACTACAA TTCCTCATGC GCTGCTGACT 
TGGAAGCCGG TCAGTGGGGG CTTTGGCCCT GTATCACCCT CTGATGCAAA AGAAAAACTG 
GATACAATCT CCCAGAAGTC TGTAGATATT CATGATACTG TTCAACCGAG ATCTTCAGAT 
GGACGGCCAC ATCAACCCAG TGCAGTGCCT ACAGGAAGTG AAGAAGTGAG CAAAGCCAGA 
TCAACAGCAA AATTTCCAGA ACAGAAGTTA TTTGCTGCTC TTCTGATCAA GTGTGTTGTA 
CAACTGGAAC TCATTCAGAC TATCGACAAC ATCGTGTTCT TCCCTGCAAC TAGCAAGAAA 
GAGGATGCAG AGAACCTAGC AGCAGCACAA AGAGATGCAG TAGATTTTGA TGTCCACGTG 
GATACCCAAG ATCAAGGGAT GTATCGTTTT CTGACATCAC AGCAGCTTTT CAAACTCCTG 
GATTGTCTGC TAGAATCTCA CAGGTTTGCT AAGGCTTTTA ATTCCAACAA TGAACAAAGA 
ACTGCTCTCT GGAAAGCAGG TTTTAAAGGC AAATCCAAGC CCAATCTTCT GAAGCAGGAG 
ACAAGCAGTC TTGCCTGTGG GTTACGCATT CTGTTCCGCA TGTACATGGA TGAAAGCCGA 
ATCAGTGCGT GGGAAGAAGT CCAGCAAAGG CTGCTAAATG TCTGCAGTGA GGCTCTGAGT 
TACTTTCTTA CTCTCACGTC AGAAAGCCAT CGGGAGGCCT GGACTAATCT ACTGCTCTTA 
TTTTTGACTA AAGTTCTGAA GATAAGTGAT GAAAGGTTCA AGGCTCACGC CTCTTTCTAC 
TATCCTCTCT TGTGTGAAAT TATGCAGTTT GACCTTATCC CTGAGCTCCG AGCTGTTCTG 
CGAAGGTTCT TCCTGCGGAT CGGCGTGGTT TTTCAGATAT CCCAACCACC GGAGCACGAG 
TCTGCAATAA GCAAGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ARFGEF1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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