NRAP cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NRAP
Entrez Gene ID	423899
Full Name	nebulin-related anchoring protein
General protein information	Preferred Names nebulin-related anchoring protein Names nebulin-related-anchoring protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004942280.3	XP_004942337.1	nebulin-related-anchoring protein isoform X2
XM_003641526.4	XP_003641574.1	nebulin-related-anchoring protein isoform X1
XM_025151837.1	XP_025007605.1	nebulin-related-anchoring protein isoform X3
XM_003641526.3	XP_003641574.1	nebulin-related-anchoring protein isoform X1
XM_004942280.2	XP_004942337.1	nebulin-related-anchoring protein isoform X2
XM_015288962.1	XP_015144448.1	nebulin-related-anchoring protein isoform X3
XM_015288962.2	XP_015144448.1	nebulin-related-anchoring protein isoform X4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nrap	NP_001107215.1
Xenopus tropicalis (tropical clawed frog)	nrap	XP_002939546.2
Bos taurus (cattle)	NRAP	NP_001091541.1
Canis lupus familiaris (dog)	NRAP	XP_851973.1
Pan troglodytes (chimpanzee)	NRAP	XP_521607.4
Mus musculus (house mouse)	Nrap	NP_032759.3
Homo sapiens (human)	NRAP	NP_001248392.1
	NRAP	XP_001090018.2
Gallus gallus (chicken)	NRAP	XP_003641574.1

Gene Ontology

The following NRAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa151689	XM_025151837.1 Latest version!	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa10474	XM_003641526.4 Latest version!	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa10475	XM_004942280.3 Latest version!	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa38032	XM_015288962.2 Latest version!	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OGa10474	XM_003641526.3	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa10475	XM_004942280.2	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa38032	XM_015288962.1	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NRAP ( XM_025151837.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025007605.1 Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X3, mRNA.

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CloneID	OGa151689
Clone ID Related Accession (Same CDS sequence)	XM_025151837.1
Accession Version	XM_025151837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	nebulin-related-anchoring protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTAA CAGTAAACAA CTTTGTTAGC CATGAGAAAA AGCCTTACTG TCAAGTGCAC 
AACCCAAAAA ACAATGCCTT CACAAGCATA TTTGAGACAC CAATAAATTT GAATGCCAAA 
AAACTATCGG AAGTGGTAAG TGAGGTAAAG TATCGAGAGG AGAGTGAGCA ATTCAAATCT 
GCCTTCCAGT GGGATGTGAG GTCCAGAGAC ATTGAAGCTG CTTATAAAGC CCAACATCTG 
TCCAGCCAGA ATGCTTACTA TGCTGCCTAT GAGGGGGGAA CGTCATGGTA CTCAGGAAAC 
ATGCCAGATC CTCAGATGGT CACAGTAACT CAGGCACAGA AGAATCTGAA TGATCTCCAA 
TATACAGAAG AATATGAGCT GCGTCAAGGC AAAGGCAGCT TTCCTGCTAT GATCACTCCA 
GGTTATCAGG TGGCTAAGAG AGCCACTCAA CTAAGCAGTA ATGTGGAATA TAGGCGGGGA 
CATGAAGAGA GAGTTTCAAA GTTCACCTCT GTTGTAGACA CTCCAGATAT ACTTCACGCA 
AAGGCTGGAG GGCAGCTTGC AAGTGATTTG AAATACACTG AAGACTTTGA AGAACAGAGA 
GGGAAAGGCA GCTTTCCTGC TATGATCACT CCAGCTTATC AAATTGCCAA GAGAGCAAAT 
GAATTAGCCA GTGATGTAAA GTATCATCAA ACGTACGAAA AAGAAATCAA GGGAAAAGCC 
AGCCATACAG CTGGAACAGA TGTTACTTTT ACAAGAGAAA ACGTAGATCA ATATGGCCAG 
GATTATATGA ATGAGTATGA AGAACGCAGA GGGAAAGGAA GCTTTCCTGC TATGATCACT 
CCAGCTTATC AAAATGCCAA GAAGGCAAAT GAATTGGCAA GTGATATAAA ATACAAGAAA 
GATCTTTCCA AGATGAAAGG TGCAGCTCAC TTTCACTCTC TAACAGCTGA AGACAACCTG 
GTCCTTAAAC AAGCCCAGAG TGCTAACAAG CTTGTTAGTG AGGTGGAATA CAAGAAGGAT 
CTTGGAAACA ACAGAGGCTA CAGCGTTAAC TACTGTGATA CTCCTCAATT CAAGAATGTG 
AGCAAGATCT CAAAATACAC CAGTGATATT AAGTACAAAG AAACGTACCA GAACCAAATG 
AAAGGCCATT ATATGGGTAT TGGTATGGAC AAGCGAATGT TACATGCCAT GAAAGTGGGC 
AACTTGGCAA GCAATATAGC CTATAAATCT GATTACAAAC ATGACGGTGT TGACTACAAT 
TACCCAGCTA CTCTCACTCC TTCATATCAG ACAACAAGGA AGTTGGTCCC TTTGAAAGAT 
GTAAACTACA GGCAAAGCAT AGACAAGATG AAGTATAGTT CTGTTGCCAG CACTCCAGAA 
ATTGCTCAAG CCAAAATAAA TGCTCAGCAG CTCAGTGATT TGAACTACCG AGCCCAGTAT 
GAGAAGACAA AAACAAATTA CACTCTACCT CAGGACATAC CTCAGTTAGT CAAGGCAAAA 
GCCAATGCAG AATTATATAG TGAGGTTAAG TACAAAGAAG GCTGGGAGAA GAGCAAGGGC 
CAGGGATTTG AAATGAAGCT TGATTCTCTG CCTTTACTTG CTGCCAAAGC TTCAAGGGAT 
CTTGCCAGTG ATATTAAGTA TAAAGAAGAA TATGAAAAAA CAAAAGGGAA AGCAATTGAA 
ACAAAAGATT CAAGACTTTT GCACTCTCTG CAGGTGGCCA AGATGAGTAG TGAGATTGCC 
TACAAAAAAG ATTTTGAGGA GAGTAAGACC CATTTCCATC TGCCTATGGA CATGGTAAAC 
CTGAGGCATG CTAAGAAGGC CCAAGCACTA GCTAGCGACT TGGATTATAG AAAGAGACTC 
CATGAATACA CAGTGGTTCC GGAAGATTTG AAGACAAAAT GGGCCAAGAA GGCCTATGGG 
CTCCAGAGTG ATCTTCAATA CAGGGAAGAC CTGATGTGGA TGAAAGGAGT TGGATGTATC 
ACAGAAGGAA GTCTTAACAT ACAGCAAGCC AAAAAGGCAG GAGATTTGGT CAGTGAGAAA 
AAATACCGAC AGAAGGTTGA TGCTCTGAAA TTCACCAGTG TGGCTGACAG CTCTCACATC 
AAACATGCCA AGAAAAGCCA GGAACTGCAA AGTGATGTTG CCTACAGGTC AGGAAAAGAG 
CAGTTTCTTC ATCAGTACAC CATCACCAAA GATGACCCTG TCTTCTTACT GGCCAAAGCA 
AATGCTGCCA ATATCAGTGA GAAACTGTAC AGAAGTAGCT GGGAGAAGCA GAAGGAAAAA 
GGATTTGTTC TTCGTCTGGA TGCTTTGTCA TTCTTGACAG CTAAGGCAAA GAGGGATCTG 
GCAAGTGATA TTAAATATAA GGAAGGTTAT GAGAAGATGA AGGGTAAGCT GATTGGAGTA 
AAAGCTGTGG AGGAAGATTC AAAGATGGCT CATTCCCTTC AAATGTCAAA ACTGCAGAGC 
GATCTGGAGT ACAAGAAGGC ATTTGAGGAC ACAAAGAATC AGTTCCAGGT CTCCATGGAC 
ATGGCTAACC TTGTCCATGC CAAAAAGGCT CAGAATTTGG CTTCAGATAA AGGATACAGG 
ACAGCCCTCC ATCACTACAC TGTCTTGCCT ACTGACAGGA AAGTGGCATG GGCCAAATGG 
GCGTATGGAT TGCAAAGTGA TAACCGATAC AGAGCAGATT TGAACTGGAT GAAAGGAGCT 
GGATGGATAG CCACTGGGTC ATTAAATGTG GAGCAGGCTA AGAAGGCAGG AGAACTCATT 
AGTGAGAAGA AGTACCGTCA GCATCCATAT GCTTTGAAGT TTACCAGTAT TAAAGACACT 
CCTGAGATGA TCCAGGCTAG AATTAGTTAT AACCAGGCTG TGGATCGGTT GTACAAGGAG 
CATGGAGAAA GCATAAAGCA TCAGTACACA CTAACAGCAG ATCTTCCTGA AATCCTTCGG 
GCTAAATTAA ACACCATGAA TATCAGTGAG ATTCGCTATA AGGAGTCCTG GCAAAAGATG 
AAGGATGGTG GATATCAGCT GAGACTCGAT GCCATTCCCT TCCAGGCAGC TAAGGCTTCG 
GGTGAAATCA TAAGCGATCA CAAGTATAAG GAGGCATTTG AGAAAATGAA AGGGCAGATG 
ATTGGTTCAT GTGGCCTGGA TGGTGATATT CGTATTGCTC ATTCAGTCCA TGCGTCATCA 
CTACAGAGTG ATGTGAAATA TAAGCAAGGT TTTGAGGATA CAAAGACTCA CTTCCATCTG 
CCACTCGATA TGATAAACTT GGTGCATGCC AGGAAAGCCC AGTCTTTGGT CAGTGAACAA 
GAATACAGAC AGCTGCTACA CCAGTACACT TCTTTGACTG ATGATCTGAG ATTGCAGTGT 
GCCAAGAATG CATATAAACT ACAGAGTGAG AATTTATATC GGTCTGACAT GAACTTTATG 
CGAGGAGTCG GATGTATTAC TCCAGGGGCC CTAGAGATTG AAGGAAAGAA GAAAGCCTCT 
GAACTCATCA GTGAGAGTAA ATATCGTCAG CAGCCACATT CCTTCAAGTA CACATCGGTG 
ACAGACTCTC CTGGTCTCCT TCATGCAAAA TTCAGCAATA TGATTGCTAA TGAGCGCCTT 
TATAAAGCAG CGGGAGAGGA CATTCAGCAT CACTATACAC CAACACTGGG TCTGCCTGAG 
TTCACACAAG CAAGAATAAA CGCAGCCAAC CTCAGTGATG TGAAATACAG GGAATCTTGG 
CATAATCTAT GTGCTCAAGG CTACAAGCTG ACAATGGAAG CACTCCCTTT CCAGACTGCG 
AGGGCCTCCA GAGAAATTGC CAGTGATTAC CAGTATAAAC ACAATTTTGT GATGGAGAGA 
GGAAAGCACA TTGGGGCCCG AAGTGTTCTG GATGATACCA GGTTGCTGCA TTGCTTGCAT 
GCTGCCAAAT TACAGAGTGA ACAGGAGTAT AAGAAAGGAT CCCAAGGAGT GTGGTCTCAG 
TACTACCTGC CCATGGACAT GGTGAATCTT GTCCATGCAA GGAAAGCCCA GGCCTTAGCA 
AGTGATCAAG AATATAGAAA GAGACTCCAT GAATTTACAG CGCTCCCAGA AGACTTGAAG 
ATGAAATGGG CCAAAAGAGC CCACATGCTA CAGAGTGAGC ATCGCTATAA ATCAGACCTG 
AACTTCATGA AAGGAGTTGG TTGGATGGCT CTGAGATCTC CGCAGATAGA AAGTGTAAAG 
AAGGCTGGCG AGCTCATCAG TGAGACCAAG TATCGTCAGA AGCCAGAAAG TTTGAAGTTC 
ACCGCGGTGG TTGACTCTCC AGATTTGATT CATGCTAAAA ATAGCTACCT CCAGTGCAAT 
GACAGGCTGT ACAAGGCTGG AGACTCTGAA GCCAGGCACA GGTATACCCT GCCTCCTGAT 
CATCCAGATT TTATCCGAGC TCGACAGAAT GCACTTAATA TCAGTGATAA AGTGTATAAA 
ACATCTTGGG AACAGACAAG AGCATCTGGC TATGATTTTA GGATTGACGC CATTCCATTC 
CAGACAGCAA AGGCTTCAAG AGAGATTGCT AGTGATTACA AATACAGAGA AGCCTTCCTT 
CGAGACAGAG GCCAGAGGAT TGGATTCTTC AGTGCAAATG ATGATGCCCA CACCAGACAT 
GTCCTCAGAG TAGGGAAACT CCAGAGCGAC AACCTGTACA GGAGCGGATA CGCCCAAAAC 
AGAGGACATT TTCAGAGTCA CCTTAACCAG CCTGGGTTCC TCCATGCTAA GAGAAGCCAG 
CAGCTTGCCA GCAATGTTAA CTACAAGCAG CCCTTGCACC AATACACTTG TGATCCTGAG 
CAGCTAAATG TGAAACATGC AAAGCAGGCC TATAAGCTGC AAAGCGATGT AAAATACAAG 
TCTGACCTGA ACTGGCTCAG AGGCATTGGC TGGACTCCTC CAGGTTCTTA CAAAGTGGAA 
AGGGCAAGAA GAGCTGCTGA GCTGGCGTAC CTTCGGGAAA TGGGGTTGCA GGCTGCTTCT 
GCTCAGTATG GACCTGAAGC TGACCAAGTG GGGCCAGAAG GATCACAGCA GGTCACAGTT 
AATCCTGATG CTTCGGAAAT TCTGCAAGTC AAGAGAAGGA AAATGCAGCT GTACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025007605.1
CDS	56..5155
Translation	MMLTVNNFVSHEKKPYCQVHNPKNNAFTSIFETPINLNAKKLSEVVSEVKYREESEQFKSAFQWDVRSRDIEAAYKAQHLSSQNAYYAAYEGGTSWYSGNMPDPQMVTVTQAQKNLNDLQYTEEYELRQGKGSFPAMITPGYQVAKRATQLSSNVEYRRGHEERVSKFTSVVDTPDILHAKAGGQLASDLKYTEDFEEQRGKGSFPAMITPAYQIAKRANELASDVKYHQTYEKEIKGKASHTAGTDVTFTRENVDQYGQDYMNEYEERRGKGSFPAMITPAYQNAKKANELASDIKYKKDLSKMKGAAHFHSLTAEDNLVLKQAQSANKLVSEVEYKKDLGNNRGYSVNYCDTPQFKNVSKISKYTSDIKYKETYQNQMKGHYMGIGMDKRMLHAMKVGNLASNIAYKSDYKHDGVDYNYPATLTPSYQTTRKLVPLKDVNYRQSIDKMKYSSVASTPEIAQAKINAQQLSDLNYRAQYEKTKTNYTLPQDIPQLVKAKANAELYSEVKYKEGWEKSKGQGFEMKLDSLPLLAAKASRDLASDIKYKEEYEKTKGKAIETKDSRLLHSLQVAKMSSEIAYKKDFEESKTHFHLPMDMVNLRHAKKAQALASDLDYRKRLHEYTVVPEDLKTKWAKKAYGLQSDLQYREDLMWMKGVGCITEGSLNIQQAKKAGDLVSEKKYRQKVDALKFTSVADSSHIKHAKKSQELQSDVAYRSGKEQFLHQYTITKDDPVFLLAKANAANISEKLYRSSWEKQKEKGFVLRLDALSFLTAKAKRDLASDIKYKEGYEKMKGKLIGVKAVEEDSKMAHSLQMSKLQSDLEYKKAFEDTKNQFQVSMDMANLVHAKKAQNLASDKGYRTALHHYTVLPTDRKVAWAKWAYGLQSDNRYRADLNWMKGAGWIATGSLNVEQAKKAGELISEKKYRQHPYALKFTSIKDTPEMIQARISYNQAVDRLYKEHGESIKHQYTLTADLPEILRAKLNTMNISEIRYKESWQKMKDGGYQLRLDAIPFQAAKASGEIISDHKYKEAFEKMKGQMIGSCGLDGDIRIAHSVHASSLQSDVKYKQGFEDTKTHFHLPLDMINLVHARKAQSLVSEQEYRQLLHQYTSLTDDLRLQCAKNAYKLQSENLYRSDMNFMRGVGCITPGALEIEGKKKASELISESKYRQQPHSFKYTSVTDSPGLLHAKFSNMIANERLYKAAGEDIQHHYTPTLGLPEFTQARINAANLSDVKYRESWHNLCAQGYKLTMEALPFQTARASREIASDYQYKHNFVMERGKHIGARSVLDDTRLLHCLHAAKLQSEQEYKKGSQGVWSQYYLPMDMVNLVHARKAQALASDQEYRKRLHEFTALPEDLKMKWAKRAHMLQSEHRYKSDLNFMKGVGWMALRSPQIESVKKAGELISETKYRQKPESLKFTAVVDSPDLIHAKNSYLQCNDRLYKAGDSEARHRYTLPPDHPDFIRARQNALNISDKVYKTSWEQTRASGYDFRIDAIPFQTAKASREIASDYKYREAFLRDRGQRIGFFSANDDAHTRHVLRVGKLQSDNLYRSGYAQNRGHFQSHLNQPGFLHAKRSQQLASNVNYKQPLHQYTCDPEQLNVKHAKQAYKLQSDVKYKSDLNWLRGIGWTPPGSYKVERARRAAELAYLREMGLQAASAQYGPEADQVGPEGSQQVTVNPDASEILQVKRRKMQLYK

Target ORF information:

RefSeq Version	XM_025151837.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025151837.1

ORF Insert Sequence:

ATGATGTTAA CAGTAAACAA CTTTGTTAGC CATGAGAAAA AGCCTTACTG TCAAGTGCAC 
AACCCAAAAA ACAATGCCTT CACAAGCATA TTTGAGACAC CAATAAATTT GAATGCCAAA 
AAACTATCGG AAGTGGTAAG TGAGGTAAAG TATCGAGAGG AGAGTGAGCA ATTCAAATCT 
GCCTTCCAGT GGGATGTGAG GTCCAGAGAC ATTGAAGCTG CTTATAAAGC CCAACATCTG 
TCCAGCCAGA ATGCTTACTA TGCTGCCTAT GAGGGGGGAA CGTCATGGTA CTCAGGAAAC 
ATGCCAGATC CTCAGATGGT CACAGTAACT CAGGCACAGA AGAATCTGAA TGATCTCCAA 
TATACAGAAG AATATGAGCT GCGTCAAGGC AAAGGCAGCT TTCCTGCTAT GATCACTCCA 
GGTTATCAGG TGGCTAAGAG AGCCACTCAA CTAAGCAGTA ATGTGGAATA TAGGCGGGGA 
CATGAAGAGA GAGTTTCAAA GTTCACCTCT GTTGTAGACA CTCCAGATAT ACTTCACGCA 
AAGGCTGGAG GGCAGCTTGC AAGTGATTTG AAATACACTG AAGACTTTGA AGAACAGAGA 
GGGAAAGGCA GCTTTCCTGC TATGATCACT CCAGCTTATC AAATTGCCAA GAGAGCAAAT 
GAATTAGCCA GTGATGTAAA GTATCATCAA ACGTACGAAA AAGAAATCAA GGGAAAAGCC 
AGCCATACAG CTGGAACAGA TGTTACTTTT ACAAGAGAAA ACGTAGATCA ATATGGCCAG 
GATTATATGA ATGAGTATGA AGAACGCAGA GGGAAAGGAA GCTTTCCTGC TATGATCACT 
CCAGCTTATC AAAATGCCAA GAAGGCAAAT GAATTGGCAA GTGATATAAA ATACAAGAAA 
GATCTTTCCA AGATGAAAGG TGCAGCTCAC TTTCACTCTC TAACAGCTGA AGACAACCTG 
GTCCTTAAAC AAGCCCAGAG TGCTAACAAG CTTGTTAGTG AGGTGGAATA CAAGAAGGAT 
CTTGGAAACA ACAGAGGCTA CAGCGTTAAC TACTGTGATA CTCCTCAATT CAAGAATGTG 
AGCAAGATCT CAAAATACAC CAGTGATATT AAGTACAAAG AAACGTACCA GAACCAAATG 
AAAGGCCATT ATATGGGTAT TGGTATGGAC AAGCGAATGT TACATGCCAT GAAAGTGGGC 
AACTTGGCAA GCAATATAGC CTATAAATCT GATTACAAAC ATGACGGTGT TGACTACAAT 
TACCCAGCTA CTCTCACTCC TTCATATCAG ACAACAAGGA AGTTGGTCCC TTTGAAAGAT 
GTAAACTACA GGCAAAGCAT AGACAAGATG AAGTATAGTT CTGTTGCCAG CACTCCAGAA 
ATTGCTCAAG CCAAAATAAA TGCTCAGCAG CTCAGTGATT TGAACTACCG AGCCCAGTAT 
GAGAAGACAA AAACAAATTA CACTCTACCT CAGGACATAC CTCAGTTAGT CAAGGCAAAA 
GCCAATGCAG AATTATATAG TGAGGTTAAG TACAAAGAAG GCTGGGAGAA GAGCAAGGGC 
CAGGGATTTG AAATGAAGCT TGATTCTCTG CCTTTACTTG CTGCCAAAGC TTCAAGGGAT 
CTTGCCAGTG ATATTAAGTA TAAAGAAGAA TATGAAAAAA CAAAAGGGAA AGCAATTGAA 
ACAAAAGATT CAAGACTTTT GCACTCTCTG CAGGTGGCCA AGATGAGTAG TGAGATTGCC 
TACAAAAAAG ATTTTGAGGA GAGTAAGACC CATTTCCATC TGCCTATGGA CATGGTAAAC 
CTGAGGCATG CTAAGAAGGC CCAAGCACTA GCTAGCGACT TGGATTATAG AAAGAGACTC 
CATGAATACA CAGTGGTTCC GGAAGATTTG AAGACAAAAT GGGCCAAGAA GGCCTATGGG 
CTCCAGAGTG ATCTTCAATA CAGGGAAGAC CTGATGTGGA TGAAAGGAGT TGGATGTATC 
ACAGAAGGAA GTCTTAACAT ACAGCAAGCC AAAAAGGCAG GAGATTTGGT CAGTGAGAAA 
AAATACCGAC AGAAGGTTGA TGCTCTGAAA TTCACCAGTG TGGCTGACAG CTCTCACATC 
AAACATGCCA AGAAAAGCCA GGAACTGCAA AGTGATGTTG CCTACAGGTC AGGAAAAGAG 
CAGTTTCTTC ATCAGTACAC CATCACCAAA GATGACCCTG TCTTCTTACT GGCCAAAGCA 
AATGCTGCCA ATATCAGTGA GAAACTGTAC AGAAGTAGCT GGGAGAAGCA GAAGGAAAAA 
GGATTTGTTC TTCGTCTGGA TGCTTTGTCA TTCTTGACAG CTAAGGCAAA GAGGGATCTG 
GCAAGTGATA TTAAATATAA GGAAGGTTAT GAGAAGATGA AGGGTAAGCT GATTGGAGTA 
AAAGCTGTGG AGGAAGATTC AAAGATGGCT CATTCCCTTC AAATGTCAAA ACTGCAGAGC 
GATCTGGAGT ACAAGAAGGC ATTTGAGGAC ACAAAGAATC AGTTCCAGGT CTCCATGGAC 
ATGGCTAACC TTGTCCATGC CAAAAAGGCT CAGAATTTGG CTTCAGATAA AGGATACAGG 
ACAGCCCTCC ATCACTACAC TGTCTTGCCT ACTGACAGGA AAGTGGCATG GGCCAAATGG 
GCGTATGGAT TGCAAAGTGA TAACCGATAC AGAGCAGATT TGAACTGGAT GAAAGGAGCT 
GGATGGATAG CCACTGGGTC ATTAAATGTG GAGCAGGCTA AGAAGGCAGG AGAACTCATT 
AGTGAGAAGA AGTACCGTCA GCATCCATAT GCTTTGAAGT TTACCAGTAT TAAAGACACT 
CCTGAGATGA TCCAGGCTAG AATTAGTTAT AACCAGGCTG TGGATCGGTT GTACAAGGAG 
CATGGAGAAA GCATAAAGCA TCAGTACACA CTAACAGCAG ATCTTCCTGA AATCCTTCGG 
GCTAAATTAA ACACCATGAA TATCAGTGAG ATTCGCTATA AGGAGTCCTG GCAAAAGATG 
AAGGATGGTG GATATCAGCT GAGACTCGAT GCCATTCCCT TCCAGGCAGC TAAGGCTTCG 
GGTGAAATCA TAAGCGATCA CAAGTATAAG GAGGCATTTG AGAAAATGAA AGGGCAGATG 
ATTGGTTCAT GTGGCCTGGA TGGTGATATT CGTATTGCTC ATTCAGTCCA TGCGTCATCA 
CTACAGAGTG ATGTGAAATA TAAGCAAGGT TTTGAGGATA CAAAGACTCA CTTCCATCTG 
CCACTCGATA TGATAAACTT GGTGCATGCC AGGAAAGCCC AGTCTTTGGT CAGTGAACAA 
GAATACAGAC AGCTGCTACA CCAGTACACT TCTTTGACTG ATGATCTGAG ATTGCAGTGT 
GCCAAGAATG CATATAAACT ACAGAGTGAG AATTTATATC GGTCTGACAT GAACTTTATG 
CGAGGAGTCG GATGTATTAC TCCAGGGGCC CTAGAGATTG AAGGAAAGAA GAAAGCCTCT 
GAACTCATCA GTGAGAGTAA ATATCGTCAG CAGCCACATT CCTTCAAGTA CACATCGGTG 
ACAGACTCTC CTGGTCTCCT TCATGCAAAA TTCAGCAATA TGATTGCTAA TGAGCGCCTT 
TATAAAGCAG CGGGAGAGGA CATTCAGCAT CACTATACAC CAACACTGGG TCTGCCTGAG 
TTCACACAAG CAAGAATAAA CGCAGCCAAC CTCAGTGATG TGAAATACAG GGAATCTTGG 
CATAATCTAT GTGCTCAAGG CTACAAGCTG ACAATGGAAG CACTCCCTTT CCAGACTGCG 
AGGGCCTCCA GAGAAATTGC CAGTGATTAC CAGTATAAAC ACAATTTTGT GATGGAGAGA 
GGAAAGCACA TTGGGGCCCG AAGTGTTCTG GATGATACCA GGTTGCTGCA TTGCTTGCAT 
GCTGCCAAAT TACAGAGTGA ACAGGAGTAT AAGAAAGGAT CCCAAGGAGT GTGGTCTCAG 
TACTACCTGC CCATGGACAT GGTGAATCTT GTCCATGCAA GGAAAGCCCA GGCCTTAGCA 
AGTGATCAAG AATATAGAAA GAGACTCCAT GAATTTACAG CGCTCCCAGA AGACTTGAAG 
ATGAAATGGG CCAAAAGAGC CCACATGCTA CAGAGTGAGC ATCGCTATAA ATCAGACCTG 
AACTTCATGA AAGGAGTTGG TTGGATGGCT CTGAGATCTC CGCAGATAGA AAGTGTAAAG 
AAGGCTGGCG AGCTCATCAG TGAGACCAAG TATCGTCAGA AGCCAGAAAG TTTGAAGTTC 
ACCGCGGTGG TTGACTCTCC AGATTTGATT CATGCTAAAA ATAGCTACCT CCAGTGCAAT 
GACAGGCTGT ACAAGGCTGG AGACTCTGAA GCCAGGCACA GGTATACCCT GCCTCCTGAT 
CATCCAGATT TTATCCGAGC TCGACAGAAT GCACTTAATA TCAGTGATAA AGTGTATAAA 
ACATCTTGGG AACAGACAAG AGCATCTGGC TATGATTTTA GGATTGACGC CATTCCATTC 
CAGACAGCAA AGGCTTCAAG AGAGATTGCT AGTGATTACA AATACAGAGA AGCCTTCCTT 
CGAGACAGAG GCCAGAGGAT TGGATTCTTC AGTGCAAATG ATGATGCCCA CACCAGACAT 
GTCCTCAGAG TAGGGAAACT CCAGAGCGAC AACCTGTACA GGAGCGGATA CGCCCAAAAC 
AGAGGACATT TTCAGAGTCA CCTTAACCAG CCTGGGTTCC TCCATGCTAA GAGAAGCCAG 
CAGCTTGCCA GCAATGTTAA CTACAAGCAG CCCTTGCACC AATACACTTG TGATCCTGAG 
CAGCTAAATG TGAAACATGC AAAGCAGGCC TATAAGCTGC AAAGCGATGT AAAATACAAG 
TCTGACCTGA ACTGGCTCAG AGGCATTGGC TGGACTCCTC CAGGTTCTTA CAAAGTGGAA 
AGGGCAAGAA GAGCTGCTGA GCTGGCGTAC CTTCGGGAAA TGGGGTTGCA GGCTGCTTCT 
GCTCAGTATG GACCTGAAGC TGACCAAGTG GGGCCAGAAG GATCACAGCA GGTCACAGTT 
AATCCTGATG CTTCGGAAAT TCTGCAAGTC AAGAGAAGGA AAATGCAGCT GTACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_003641526.4 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_003641574.1 Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X1, mRNA.

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CloneID	OGa10474
Clone ID Related Accession (Same CDS sequence)	XM_003641526.4 , XM_003641526.3
Accession Version	XM_003641526.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	nebulin-related-anchoring protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_003641526.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA GTGAGGTAAA GTATCGAGAG GAGAGTGAGC AATTCAAATC TGCCTTCCAG 
TGGGATGTGA GGTCCAGAGA CATTGAAGCT GCTTATAAAG CCCAACATCT GTCCAGCCAG 
AATGCTTACT ATGCTGCCTA TGAGGGGGGA ACGTCATGGT ACTCAGGAAA CATGCCAGAT 
CCTCAGATGG TCACAGTAAC TCAGGCACAG AAGAATCTGA ATGATCTCCA ATATACAGAA 
GAATATGAGC TGCGTCAAGG CAAAGGCAGC TTTCCTGCTA TGATCACTCC AGGTTATCAG 
GTGGCTAAGA GAGCCACTCA ACTAAGCAGT AATGTGGAAT ATAGGCGGGG ACATGAAGAG 
AGAGTTTCAA AGTTCACCTC TGTTGTAGAC ACTCCAGATA TACTTCACGC AAAGGCTGGA 
GGGCAGCTTG CAAGTGATTT GAAATACACT GAAGACTTTG AAGAACAGAG AGGGAAAGGC 
AGCTTTCCTG CTATGATCAC TCCAGCTTAT CAAATTGCCA AGAGAGCAAA TGAATTAGCC 
AGTGATGTAA AGTATCATCA AACGTACGAA AAAGAAATCA AGGGAAAAGC CAGCCATACA 
GCTGGAACAG ATGTTACTTT TACAAGAGAA AACGTAGATC AATATGGCCA GGATTATATG 
AATGAGTATG AAGAACGCAG AGGGAAAGGA AGCTTTCCTG CTATGATCAC TCCAGCTTAT 
CAAAATGCCA AGAAGGCAAA TGAATTGGCA AGTGATATAA AATACAAGAA AGATCTTTCC 
AAGATGAAAG GTGCAGCTCA CTTTCACTCT CTAACAGCTG AAGACAACCT GGTCCTTAAA 
CAAGCCCAGA GTGCTAACAA GCTTGTTAGT GAGGTGGAAT ACAAGAAGGA TCTTGGAAAC 
AACAGAGGCT ACAGCGTTAA CTACTGTGAT ACTCCTCAAT TCAAGAATGT GAGCAAGATC 
TCAAAATACA CCAGTGATAT TAAGTACAAA GAAACGTACC AGAACCAAAT GAAAGGCCAT 
TATATGGGTA TTGGTATGGA CAAGCGAATG TTACATGCCA TGAAAGTGGG CAACTTGGCA 
AGCAATATAG CCTATAAATC TGATTACAAA CATGACGGTG TTGACTACAA TTACCCAGCT 
ACTCTCACTC CTTCATATCA GACAACAAGG AAGTTGGTCC CTTTGAAAGA TGTAAACTAC 
AGGCAAAGCA TAGACAAGAT GAAGTATAGT TCTGTTGCCA GCACTCCAGA AATTGCTCAA 
GCCAAAATAA ATGCTCAGCA GCTCAGTGAT TTGAACTACC GAGCCCAGTA TGAGAAGACA 
AAAACAAATT ACACTCTACC TCAGGACATA CCTCAGTTAG TCAAGGCAAA AGCCAATGCA 
GAATTATATA GTGAGGTTAA GTACAAAGAA GGCTGGGAGA AGAGCAAGGG CCAGGGATTT 
GAAATGAAGC TTGATTCTCT GCCTTTACTT GCTGCCAAAG CTTCAAGGGA TCTTGCCAGT 
GATATTAAGT ATAAAGAAGA ATATGAAAAA ACAAAAGGGA AAGCAATTGA AACAAAAGAT 
TCAAGACTTT TGCACTCTCT GCAGGTGGCC AAGATGAGTA GTGAGATTGC CTACAAAAAA 
GATTTTGAGG AGAGTAAGAC CCATTTCCAT CTGCCTATGG ACATGGTAAA CCTGAGGCAT 
GCTAAGAAGG CCCAAGCACT AGCTAGCGAC TTGGATTATA GAAAGAGACT CCATGAATAC 
ACAGTGGTTC CGGAAGATTT GAAGACAAAA TGGGCCAAGA AGGCCTATGG GCTCCAGAGT 
GATCTTCAAT ACAGGGAAGA CCTGATGTGG ATGAAAGGAG TTGGATGTAT CACAGAAGGA 
AGTCTTAACA TACAGCAAGC CAAAAAGGCA GGAGATTTGG TCAGTGAGAA AAAATACCGA 
CAGAAGGTTG ATGCTCTGAA ATTCACCAGT GTGGCTGACA GCTCTCACAT CAAACATGCC 
AAGAAAAGCC AGGAACTGCA AAGTGATGTT GCCTACAGGT CAGGAAAAGA GCAGTTTCTT 
CATCAGTACA CCATCACCAA AGATGACCCT GTCTTCTTAC TGGCCAAAGC AAATGCTGCC 
AATATCAGTG AGAAACTGTA CAGAAGTAGC TGGGAGAAGC AGAAGGAAAA AGGATTTGTT 
CTTCGTCTGG ATGCTTTGTC ATTCTTGACA GCTAAGGCAA AGAGGGATCT GGCAAGTGAT 
ATTAAATATA AGGAAGGTTA TGAGAAGATG AAGGGTAAGC TGATTGGAGT AAAAGCTGTG 
GAGGAAGATT CAAAGATGGC TCATTCCCTT CAAATGTCAA AACTGCAGAG CGATCTGGAG 
TACAAGAAGG CATTTGAGGA CACAAAGAAT CAGTTCCAGG TCTCCATGGA CATGGCTAAC 
CTTGTCCATG CCAAAAAGGC TCAGAATTTG GCTTCAGATA AAGGATACAG GACAGCCCTC 
CATCACTACA CTGTCTTGCC TACTGACAGG AAAGTGGCAT GGGCCAAATG GGCGTATGGA 
TTGCAAAGTG ATAACCGATA CAGAGCAGAT TTGAACTGGA TGAAAGGAGC TGGATGGATA 
GCCACTGGGT CATTAAATGT GGAGCAGGCT AAGAAGGCAG GAGAACTCAT TAGTGAGAAG 
AAGTACCGTC AGCATCCATA TGCTTTGAAG TTTACCAGTA TTAAAGACAC TCCTGAGATG 
ATCCAGGCTA GAATTAGTTA TAACCAGGCT GTGGATCGGT TGTACAAGGA GCATGGAGAA 
AGCATAAAGC ATCAGTACAC ACTAACAGCA GATCTTCCTG AAATCCTTCG GGCTAAATTA 
AACACCATGA ATATCAGTGA GATTCGCTAT AAGGAGTCCT GGCAAAAGAT GAAGGATGGT 
GGATATCAGC TGAGACTCGA TGCCATTCCC TTCCAGGCAG CTAAGGCTTC GGGTGAAATC 
ATAAGCGATC ACAAGTATAA GGAGGCATTT GAGAAAATGA AAGGGCAGAT GATTGGTTCA 
TGTGGCCTGG ATGGTGATAT TCGTATTGCT CATTCAGTCC ATGCGTCATC ACTACAGAGT 
GATGTGAAAT ATAAGCAAGG TTTTGAGGAT ACAAAGACTC ACTTCCATCT GCCACTCGAT 
ATGATAAACT TGGTGCATGC CAGGAAAGCC CAGTCTTTGG TCAGTGAACA AGAATACAGA 
CAGCTGCTAC ACCAGTACAC TTCTTTGACT GATGATCTGA GATTGCAGTG TGCCAAGAAT 
GCATATAAAC TACAGAGTGA GAATTTATAT CGGTCTGACA TGAACTTTAT GCGAGGAGTC 
GGATGTATTA CTCCAGGGGC CCTAGAGATT GAAGGAAAGA AGAAAGCCTC TGAACTCATC 
AGTGAGAGTA AATATCGTCA GCAGCCACAT TCCTTCAAGT ACACATCGGT GACAGACTCT 
CCTGGTCTCC TTCATGCAAA ATTCAGCAAT ATGATTGCTA ATGAGCGCCT TTATAAAGCA 
GCGGGAGAGG ACATTCAGCA TCACTATACA CCAACACTGG GTCTGCCTGA GTTCACACAA 
GCAAGAATAA ACGCAGCCAA CCTCAGTGAT GTGAAATACA GGGAATCTTG GCATAATCTA 
TGTGCTCAAG GCTACAAGCT GACAATGGAA GCACTCCCTT TCCAGACTGC GAGGGCCTCC 
AGAGAAATTG CCAGTGATTA CCAGTATAAA CACAATTTTG TGATGGAGAG AGGAAAGCAC 
ATTGGGGCCC GAAGTGTTCT GGATGATACC AGGTTGCTGC ATTGCTTGCA TGCTGCCAAA 
TTACAGAGTG AACAGGAGTA TAAGAAAGGA TCCCAAGGAG TGTGGTCTCA GTACTACCTG 
CCCATGGACA TGGTGAATCT TGTCCATGCA AGGAAAGCCC AGGCCTTAGC AAGTGATCAA 
GAATATAGAA AGAGACTCCA TGAATTTACA GCGCTCCCAG AAGACTTGAA GATGAAATGG 
GCCAAAAGAG CCCACATGCT ACAGAGTGAG CATCGCTATA AATCAGACCT GAACTTCATG 
AAAGGAGTTG GTTGGATGGC TCTGAGATCT CCGCAGATAG AAAGTGTAAA GAAGGCTGGC 
GAGCTCATCA GTGAGACCAA GTATCGTCAG AAGCCAGAAA GTTTGAAGTT CACCGCGGTG 
GTTGACTCTC CAGATTTGAT TCATGCTAAA AATAGCTACC TCCAGTGCAA TGACAGGCTG 
TACAAGGCTG GAGACTCTGA AGCCAGGCAC AGGTATACCC TGCCTCCTGA TCATCCAGAT 
TTTATCCGAG CTCGACAGAA TGCACTTAAT ATCAGTGATA AAGTGTATAA AACATCTTGG 
GAACAGACAA GAGCATCTGG CTATGATTTT AGGATTGACG CCATTCCATT CCAGACAGCA 
AAGGCTTCAA GAGAGATTGC TAGTGATTAC AAATACAGAG AAGCCTTCCT TCGAGACAGA 
GGCCAGAGGA TTGGATTCTT CAGTGCAAAT GATGATGCCC ACACCAGACA TGTCCTCAGA 
GTAGGGAAAC TCCAGAGCGA CAACCTGTAC AGGAGCGGAT ACGCCCAAAA CAGAGGACAT 
TTTCAGAGTC ACCTTAACCA GCCTGGGTTC CTCCATGCTA AGAGAAGCCA GCAGCTTGCC 
AGCAATGTTA ACTACAAGCA GCCCTTGCAC CAATACACTT GTGATCCTGA GCAGCTAAAT 
GTGAAACATG CAAAGCAGGC CTATAAGCTG CAAAGCGATG TAAAATACAA GTCTGACCTG 
AACTGGCTCA GAGGCATTGG CTGGACTCCT CCAGGTTCTT ACAAAGTGGA AAGGGCAAGA 
AGAGCTGCTG AGCTGGCGTA CCTTCGGGAA ATGGGGTTGC AGGCTGCTTC TGCTCAGTAT 
GGACCTGAAG CTGACCAAGT GGGGCCAGAA GGATCACAGC AGGTCACAGT TAATCCTGAT 
GCTTCGGAAA TTCTGCAAGT CAAGAGAAGG AAAATGCAGC TGTACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003641574.1
CDS	198..5408
Translation	MNVQPCARCGYGVYPAEKINCIDQTWHKACFHCEVCKMMLTVNNFVSHEKKPYCQVHNPKNNAFTSIFETPINLNAKKLSEVVSEVKYREESEQFKSAFQWDVRSRDIEAAYKAQHLSSQNAYYAAYEGGTSWYSGNMPDPQMVTVTQAQKNLNDLQYTEEYELRQGKGSFPAMITPGYQVAKRATQLSSNVEYRRGHEERVSKFTSVVDTPDILHAKAGGQLASDLKYTEDFEEQRGKGSFPAMITPAYQIAKRANELASDVKYHQTYEKEIKGKASHTAGTDVTFTRENVDQYGQDYMNEYEERRGKGSFPAMITPAYQNAKKANELASDIKYKKDLSKMKGAAHFHSLTAEDNLVLKQAQSANKLVSEVEYKKDLGNNRGYSVNYCDTPQFKNVSKISKYTSDIKYKETYQNQMKGHYMGIGMDKRMLHAMKVGNLASNIAYKSDYKHDGVDYNYPATLTPSYQTTRKLVPLKDVNYRQSIDKMKYSSVASTPEIAQAKINAQQLSDLNYRAQYEKTKTNYTLPQDIPQLVKAKANAELYSEVKYKEGWEKSKGQGFEMKLDSLPLLAAKASRDLASDIKYKEEYEKTKGKAIETKDSRLLHSLQVAKMSSEIAYKKDFEESKTHFHLPMDMVNLRHAKKAQALASDLDYRKRLHEYTVVPEDLKTKWAKKAYGLQSDLQYREDLMWMKGVGCITEGSLNIQQAKKAGDLVSEKKYRQKVDALKFTSVADSSHIKHAKKSQELQSDVAYRSGKEQFLHQYTITKDDPVFLLAKANAANISEKLYRSSWEKQKEKGFVLRLDALSFLTAKAKRDLASDIKYKEGYEKMKGKLIGVKAVEEDSKMAHSLQMSKLQSDLEYKKAFEDTKNQFQVSMDMANLVHAKKAQNLASDKGYRTALHHYTVLPTDRKVAWAKWAYGLQSDNRYRADLNWMKGAGWIATGSLNVEQAKKAGELISEKKYRQHPYALKFTSIKDTPEMIQARISYNQAVDRLYKEHGESIKHQYTLTADLPEILRAKLNTMNISEIRYKESWQKMKDGGYQLRLDAIPFQAAKASGEIISDHKYKEAFEKMKGQMIGSCGLDGDIRIAHSVHASSLQSDVKYKQGFEDTKTHFHLPLDMINLVHARKAQSLVSEQEYRQLLHQYTSLTDDLRLQCAKNAYKLQSENLYRSDMNFMRGVGCITPGALEIEGKKKASELISESKYRQQPHSFKYTSVTDSPGLLHAKFSNMIANERLYKAAGEDIQHHYTPTLGLPEFTQARINAANLSDVKYRESWHNLCAQGYKLTMEALPFQTARASREIASDYQYKHNFVMERGKHIGARSVLDDTRLLHCLHAAKLQSEQEYKKGSQGVWSQYYLPMDMVNLVHARKAQALASDQEYRKRLHEFTALPEDLKMKWAKRAHMLQSEHRYKSDLNFMKGVGWMALRSPQIESVKKAGELISETKYRQKPESLKFTAVVDSPDLIHAKNSYLQCNDRLYKAGDSEARHRYTLPPDHPDFIRARQNALNISDKVYKTSWEQTRASGYDFRIDAIPFQTAKASREIASDYKYREAFLRDRGQRIGFFSANDDAHTRHVLRVGKLQSDNLYRSGYAQNRGHFQSHLNQPGFLHAKRSQQLASNVNYKQPLHQYTCDPEQLNVKHAKQAYKLQSDVKYKSDLNWLRGIGWTPPGSYKVERARRAAELAYLREMGLQAASAQYGPEADQVGPEGSQQVTVNPDASEILQVKRRKMQLYK

Target ORF information:

RefSeq Version	XM_003641526.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003641526.4

ORF Insert Sequence:

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA GTGAGGTAAA GTATCGAGAG GAGAGTGAGC AATTCAAATC TGCCTTCCAG 
TGGGATGTGA GGTCCAGAGA CATTGAAGCT GCTTATAAAG CCCAACATCT GTCCAGCCAG 
AATGCTTACT ATGCTGCCTA TGAGGGGGGA ACGTCATGGT ACTCAGGAAA CATGCCAGAT 
CCTCAGATGG TCACAGTAAC TCAGGCACAG AAGAATCTGA ATGATCTCCA ATATACAGAA 
GAATATGAGC TGCGTCAAGG CAAAGGCAGC TTTCCTGCTA TGATCACTCC AGGTTATCAG 
GTGGCTAAGA GAGCCACTCA ACTAAGCAGT AATGTGGAAT ATAGGCGGGG ACATGAAGAG 
AGAGTTTCAA AGTTCACCTC TGTTGTAGAC ACTCCAGATA TACTTCACGC AAAGGCTGGA 
GGGCAGCTTG CAAGTGATTT GAAATACACT GAAGACTTTG AAGAACAGAG AGGGAAAGGC 
AGCTTTCCTG CTATGATCAC TCCAGCTTAT CAAATTGCCA AGAGAGCAAA TGAATTAGCC 
AGTGATGTAA AGTATCATCA AACGTACGAA AAAGAAATCA AGGGAAAAGC CAGCCATACA 
GCTGGAACAG ATGTTACTTT TACAAGAGAA AACGTAGATC AATATGGCCA GGATTATATG 
AATGAGTATG AAGAACGCAG AGGGAAAGGA AGCTTTCCTG CTATGATCAC TCCAGCTTAT 
CAAAATGCCA AGAAGGCAAA TGAATTGGCA AGTGATATAA AATACAAGAA AGATCTTTCC 
AAGATGAAAG GTGCAGCTCA CTTTCACTCT CTAACAGCTG AAGACAACCT GGTCCTTAAA 
CAAGCCCAGA GTGCTAACAA GCTTGTTAGT GAGGTGGAAT ACAAGAAGGA TCTTGGAAAC 
AACAGAGGCT ACAGCGTTAA CTACTGTGAT ACTCCTCAAT TCAAGAATGT GAGCAAGATC 
TCAAAATACA CCAGTGATAT TAAGTACAAA GAAACGTACC AGAACCAAAT GAAAGGCCAT 
TATATGGGTA TTGGTATGGA CAAGCGAATG TTACATGCCA TGAAAGTGGG CAACTTGGCA 
AGCAATATAG CCTATAAATC TGATTACAAA CATGACGGTG TTGACTACAA TTACCCAGCT 
ACTCTCACTC CTTCATATCA GACAACAAGG AAGTTGGTCC CTTTGAAAGA TGTAAACTAC 
AGGCAAAGCA TAGACAAGAT GAAGTATAGT TCTGTTGCCA GCACTCCAGA AATTGCTCAA 
GCCAAAATAA ATGCTCAGCA GCTCAGTGAT TTGAACTACC GAGCCCAGTA TGAGAAGACA 
AAAACAAATT ACACTCTACC TCAGGACATA CCTCAGTTAG TCAAGGCAAA AGCCAATGCA 
GAATTATATA GTGAGGTTAA GTACAAAGAA GGCTGGGAGA AGAGCAAGGG CCAGGGATTT 
GAAATGAAGC TTGATTCTCT GCCTTTACTT GCTGCCAAAG CTTCAAGGGA TCTTGCCAGT 
GATATTAAGT ATAAAGAAGA ATATGAAAAA ACAAAAGGGA AAGCAATTGA AACAAAAGAT 
TCAAGACTTT TGCACTCTCT GCAGGTGGCC AAGATGAGTA GTGAGATTGC CTACAAAAAA 
GATTTTGAGG AGAGTAAGAC CCATTTCCAT CTGCCTATGG ACATGGTAAA CCTGAGGCAT 
GCTAAGAAGG CCCAAGCACT AGCTAGCGAC TTGGATTATA GAAAGAGACT CCATGAATAC 
ACAGTGGTTC CGGAAGATTT GAAGACAAAA TGGGCCAAGA AGGCCTATGG GCTCCAGAGT 
GATCTTCAAT ACAGGGAAGA CCTGATGTGG ATGAAAGGAG TTGGATGTAT CACAGAAGGA 
AGTCTTAACA TACAGCAAGC CAAAAAGGCA GGAGATTTGG TCAGTGAGAA AAAATACCGA 
CAGAAGGTTG ATGCTCTGAA ATTCACCAGT GTGGCTGACA GCTCTCACAT CAAACATGCC 
AAGAAAAGCC AGGAACTGCA AAGTGATGTT GCCTACAGGT CAGGAAAAGA GCAGTTTCTT 
CATCAGTACA CCATCACCAA AGATGACCCT GTCTTCTTAC TGGCCAAAGC AAATGCTGCC 
AATATCAGTG AGAAACTGTA CAGAAGTAGC TGGGAGAAGC AGAAGGAAAA AGGATTTGTT 
CTTCGTCTGG ATGCTTTGTC ATTCTTGACA GCTAAGGCAA AGAGGGATCT GGCAAGTGAT 
ATTAAATATA AGGAAGGTTA TGAGAAGATG AAGGGTAAGC TGATTGGAGT AAAAGCTGTG 
GAGGAAGATT CAAAGATGGC TCATTCCCTT CAAATGTCAA AACTGCAGAG CGATCTGGAG 
TACAAGAAGG CATTTGAGGA CACAAAGAAT CAGTTCCAGG TCTCCATGGA CATGGCTAAC 
CTTGTCCATG CCAAAAAGGC TCAGAATTTG GCTTCAGATA AAGGATACAG GACAGCCCTC 
CATCACTACA CTGTCTTGCC TACTGACAGG AAAGTGGCAT GGGCCAAATG GGCGTATGGA 
TTGCAAAGTG ATAACCGATA CAGAGCAGAT TTGAACTGGA TGAAAGGAGC TGGATGGATA 
GCCACTGGGT CATTAAATGT GGAGCAGGCT AAGAAGGCAG GAGAACTCAT TAGTGAGAAG 
AAGTACCGTC AGCATCCATA TGCTTTGAAG TTTACCAGTA TTAAAGACAC TCCTGAGATG 
ATCCAGGCTA GAATTAGTTA TAACCAGGCT GTGGATCGGT TGTACAAGGA GCATGGAGAA 
AGCATAAAGC ATCAGTACAC ACTAACAGCA GATCTTCCTG AAATCCTTCG GGCTAAATTA 
AACACCATGA ATATCAGTGA GATTCGCTAT AAGGAGTCCT GGCAAAAGAT GAAGGATGGT 
GGATATCAGC TGAGACTCGA TGCCATTCCC TTCCAGGCAG CTAAGGCTTC GGGTGAAATC 
ATAAGCGATC ACAAGTATAA GGAGGCATTT GAGAAAATGA AAGGGCAGAT GATTGGTTCA 
TGTGGCCTGG ATGGTGATAT TCGTATTGCT CATTCAGTCC ATGCGTCATC ACTACAGAGT 
GATGTGAAAT ATAAGCAAGG TTTTGAGGAT ACAAAGACTC ACTTCCATCT GCCACTCGAT 
ATGATAAACT TGGTGCATGC CAGGAAAGCC CAGTCTTTGG TCAGTGAACA AGAATACAGA 
CAGCTGCTAC ACCAGTACAC TTCTTTGACT GATGATCTGA GATTGCAGTG TGCCAAGAAT 
GCATATAAAC TACAGAGTGA GAATTTATAT CGGTCTGACA TGAACTTTAT GCGAGGAGTC 
GGATGTATTA CTCCAGGGGC CCTAGAGATT GAAGGAAAGA AGAAAGCCTC TGAACTCATC 
AGTGAGAGTA AATATCGTCA GCAGCCACAT TCCTTCAAGT ACACATCGGT GACAGACTCT 
CCTGGTCTCC TTCATGCAAA ATTCAGCAAT ATGATTGCTA ATGAGCGCCT TTATAAAGCA 
GCGGGAGAGG ACATTCAGCA TCACTATACA CCAACACTGG GTCTGCCTGA GTTCACACAA 
GCAAGAATAA ACGCAGCCAA CCTCAGTGAT GTGAAATACA GGGAATCTTG GCATAATCTA 
TGTGCTCAAG GCTACAAGCT GACAATGGAA GCACTCCCTT TCCAGACTGC GAGGGCCTCC 
AGAGAAATTG CCAGTGATTA CCAGTATAAA CACAATTTTG TGATGGAGAG AGGAAAGCAC 
ATTGGGGCCC GAAGTGTTCT GGATGATACC AGGTTGCTGC ATTGCTTGCA TGCTGCCAAA 
TTACAGAGTG AACAGGAGTA TAAGAAAGGA TCCCAAGGAG TGTGGTCTCA GTACTACCTG 
CCCATGGACA TGGTGAATCT TGTCCATGCA AGGAAAGCCC AGGCCTTAGC AAGTGATCAA 
GAATATAGAA AGAGACTCCA TGAATTTACA GCGCTCCCAG AAGACTTGAA GATGAAATGG 
GCCAAAAGAG CCCACATGCT ACAGAGTGAG CATCGCTATA AATCAGACCT GAACTTCATG 
AAAGGAGTTG GTTGGATGGC TCTGAGATCT CCGCAGATAG AAAGTGTAAA GAAGGCTGGC 
GAGCTCATCA GTGAGACCAA GTATCGTCAG AAGCCAGAAA GTTTGAAGTT CACCGCGGTG 
GTTGACTCTC CAGATTTGAT TCATGCTAAA AATAGCTACC TCCAGTGCAA TGACAGGCTG 
TACAAGGCTG GAGACTCTGA AGCCAGGCAC AGGTATACCC TGCCTCCTGA TCATCCAGAT 
TTTATCCGAG CTCGACAGAA TGCACTTAAT ATCAGTGATA AAGTGTATAA AACATCTTGG 
GAACAGACAA GAGCATCTGG CTATGATTTT AGGATTGACG CCATTCCATT CCAGACAGCA 
AAGGCTTCAA GAGAGATTGC TAGTGATTAC AAATACAGAG AAGCCTTCCT TCGAGACAGA 
GGCCAGAGGA TTGGATTCTT CAGTGCAAAT GATGATGCCC ACACCAGACA TGTCCTCAGA 
GTAGGGAAAC TCCAGAGCGA CAACCTGTAC AGGAGCGGAT ACGCCCAAAA CAGAGGACAT 
TTTCAGAGTC ACCTTAACCA GCCTGGGTTC CTCCATGCTA AGAGAAGCCA GCAGCTTGCC 
AGCAATGTTA ACTACAAGCA GCCCTTGCAC CAATACACTT GTGATCCTGA GCAGCTAAAT 
GTGAAACATG CAAAGCAGGC CTATAAGCTG CAAAGCGATG TAAAATACAA GTCTGACCTG 
AACTGGCTCA GAGGCATTGG CTGGACTCCT CCAGGTTCTT ACAAAGTGGA AAGGGCAAGA 
AGAGCTGCTG AGCTGGCGTA CCTTCGGGAA ATGGGGTTGC AGGCTGCTTC TGCTCAGTAT 
GGACCTGAAG CTGACCAAGT GGGGCCAGAA GGATCACAGC AGGTCACAGT TAATCCTGAT 
GCTTCGGAAA TTCTGCAAGT CAAGAGAAGG AAAATGCAGC TGTACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_003641526.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_003641574.1 Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa10474
Clone ID Related Accession (Same CDS sequence)	XM_003641526.4 , XM_003641526.3
Accession Version	XM_003641526.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	nebulin-related-anchoring protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455898.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_003641526.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA GTGAGGTAAA GTATCGAGAG GAGAGTGAGC AATTCAAATC TGCCTTCCAG 
TGGGATGTGA GGTCCAGAGA CATTGAAGCT GCTTATAAAG CCCAACATCT GTCCAGCCAG 
AATGCTTACT ATGCTGCCTA TGAGGGGGGA ACGTCATGGT ACTCAGGAAA CATGCCAGAT 
CCTCAGATGG TCACAGTAAC TCAGGCACAG AAGAATCTGA ATGATCTCCA ATATACAGAA 
GAATATGAGC TGCGTCAAGG CAAAGGCAGC TTTCCTGCTA TGATCACTCC AGGTTATCAG 
GTGGCTAAGA GAGCCACTCA ACTAAGCAGT AATGTGGAAT ATAGGCGGGG ACATGAAGAG 
AGAGTTTCAA AGTTCACCTC TGTTGTAGAC ACTCCAGATA TACTTCACGC AAAGGCTGGA 
GGGCAGCTTG CAAGTGATTT GAAATACACT GAAGACTTTG AAGAACAGAG AGGGAAAGGC 
AGCTTTCCTG CTATGATCAC TCCAGCTTAT CAAATTGCCA AGAGAGCAAA TGAATTAGCC 
AGTGATGTAA AGTATCATCA AACGTACGAA AAAGAAATCA AGGGAAAAGC CAGCCATACA 
GCTGGAACAG ATGTTACTTT TACAAGAGAA AACGTAGATC AATATGGCCA GGATTATATG 
AATGAGTATG AAGAACGCAG AGGGAAAGGA AGCTTTCCTG CTATGATCAC TCCAGCTTAT 
CAAAATGCCA AGAAGGCAAA TGAATTGGCA AGTGATATAA AATACAAGAA AGATCTTTCC 
AAGATGAAAG GTGCAGCTCA CTTTCACTCT CTAACAGCTG AAGACAACCT GGTCCTTAAA 
CAAGCCCAGA GTGCTAACAA GCTTGTTAGT GAGGTGGAAT ACAAGAAGGA TCTTGGAAAC 
AACAGAGGCT ACAGCGTTAA CTACTGTGAT ACTCCTCAAT TCAAGAATGT GAGCAAGATC 
TCAAAATACA CCAGTGATAT TAAGTACAAA GAAACGTACC AGAACCAAAT GAAAGGCCAT 
TATATGGGTA TTGGTATGGA CAAGCGAATG TTACATGCCA TGAAAGTGGG CAACTTGGCA 
AGCAATATAG CCTATAAATC TGATTACAAA CATGACGGTG TTGACTACAA TTACCCAGCT 
ACTCTCACTC CTTCATATCA GACAACAAGG AAGTTGGTCC CTTTGAAAGA TGTAAACTAC 
AGGCAAAGCA TAGACAAGAT GAAGTATAGT TCTGTTGCCA GCACTCCAGA AATTGCTCAA 
GCCAAAATAA ATGCTCAGCA GCTCAGTGAT TTGAACTACC GAGCCCAGTA TGAGAAGACA 
AAAACAAATT ACACTCTACC TCAGGACATA CCTCAGTTAG TCAAGGCAAA AGCCAATGCA 
GAATTATATA GTGAGGTTAA GTACAAAGAA GGCTGGGAGA AGAGCAAGGG CCAGGGATTT 
GAAATGAAGC TTGATTCTCT GCCTTTACTT GCTGCCAAAG CTTCAAGGGA TCTTGCCAGT 
GATATTAAGT ATAAAGAAGA ATATGAAAAA ACAAAAGGGA AAGCAATTGA AACAAAAGAT 
TCAAGACTTT TGCACTCTCT GCAGGTGGCC AAGATGAGTA GTGAGATTGC CTACAAAAAA 
GATTTTGAGG AGAGTAAGAC CCATTTCCAT CTGCCTATGG ACATGGTAAA CCTGAGGCAT 
GCTAAGAAGG CCCAAGCACT AGCTAGCGAC TTGGATTATA GAAAGAGACT CCATGAATAC 
ACAGTGGTTC CGGAAGATTT GAAGACAAAA TGGGCCAAGA AGGCCTATGG GCTCCAGAGT 
GATCTTCAAT ACAGGGAAGA CCTGATGTGG ATGAAAGGAG TTGGATGTAT CACAGAAGGA 
AGTCTTAACA TACAGCAAGC CAAAAAGGCA GGAGATTTGG TCAGTGAGAA AAAATACCGA 
CAGAAGGTTG ATGCTCTGAA ATTCACCAGT GTGGCTGACA GCTCTCACAT CAAACATGCC 
AAGAAAAGCC AGGAACTGCA AAGTGATGTT GCCTACAGGT CAGGAAAAGA GCAGTTTCTT 
CATCAGTACA CCATCACCAA AGATGACCCT GTCTTCTTAC TGGCCAAAGC AAATGCTGCC 
AATATCAGTG AGAAACTGTA CAGAAGTAGC TGGGAGAAGC AGAAGGAAAA AGGATTTGTT 
CTTCGTCTGG ATGCTTTGTC ATTCTTGACA GCTAAGGCAA AGAGGGATCT GGCAAGTGAT 
ATTAAATATA AGGAAGGTTA TGAGAAGATG AAGGGTAAGC TGATTGGAGT AAAAGCTGTG 
GAGGAAGATT CAAAGATGGC TCATTCCCTT CAAATGTCAA AACTGCAGAG CGATCTGGAG 
TACAAGAAGG CATTTGAGGA CACAAAGAAT CAGTTCCAGG TCTCCATGGA CATGGCTAAC 
CTTGTCCATG CCAAAAAGGC TCAGAATTTG GCTTCAGATA AAGGATACAG GACAGCCCTC 
CATCACTACA CTGTCTTGCC TACTGACAGG AAAGTGGCAT GGGCCAAATG GGCGTATGGA 
TTGCAAAGTG ATAACCGATA CAGAGCAGAT TTGAACTGGA TGAAAGGAGC TGGATGGATA 
GCCACTGGGT CATTAAATGT GGAGCAGGCT AAGAAGGCAG GAGAACTCAT TAGTGAGAAG 
AAGTACCGTC AGCATCCATA TGCTTTGAAG TTTACCAGTA TTAAAGACAC TCCTGAGATG 
ATCCAGGCTA GAATTAGTTA TAACCAGGCT GTGGATCGGT TGTACAAGGA GCATGGAGAA 
AGCATAAAGC ATCAGTACAC ACTAACAGCA GATCTTCCTG AAATCCTTCG GGCTAAATTA 
AACACCATGA ATATCAGTGA GATTCGCTAT AAGGAGTCCT GGCAAAAGAT GAAGGATGGT 
GGATATCAGC TGAGACTCGA TGCCATTCCC TTCCAGGCAG CTAAGGCTTC GGGTGAAATC 
ATAAGCGATC ACAAGTATAA GGAGGCATTT GAGAAAATGA AAGGGCAGAT GATTGGTTCA 
TGTGGCCTGG ATGGTGATAT TCGTATTGCT CATTCAGTCC ATGCGTCATC ACTACAGAGT 
GATGTGAAAT ATAAGCAAGG TTTTGAGGAT ACAAAGACTC ACTTCCATCT GCCACTCGAT 
ATGATAAACT TGGTGCATGC CAGGAAAGCC CAGTCTTTGG TCAGTGAACA AGAATACAGA 
CAGCTGCTAC ACCAGTACAC TTCTTTGACT GATGATCTGA GATTGCAGTG TGCCAAGAAT 
GCATATAAAC TACAGAGTGA GAATTTATAT CGGTCTGACA TGAACTTTAT GCGAGGAGTC 
GGATGTATTA CTCCAGGGGC CCTAGAGATT GAAGGAAAGA AGAAAGCCTC TGAACTCATC 
AGTGAGAGTA AATATCGTCA GCAGCCACAT TCCTTCAAGT ACACATCGGT GACAGACTCT 
CCTGGTCTCC TTCATGCAAA ATTCAGCAAT ATGATTGCTA ATGAGCGCCT TTATAAAGCA 
GCGGGAGAGG ACATTCAGCA TCACTATACA CCAACACTGG GTCTGCCTGA GTTCACACAA 
GCAAGAATAA ACGCAGCCAA CCTCAGTGAT GTGAAATACA GGGAATCTTG GCATAATCTA 
TGTGCTCAAG GCTACAAGCT GACAATGGAA GCACTCCCTT TCCAGACTGC GAGGGCCTCC 
AGAGAAATTG CCAGTGATTA CCAGTATAAA CACAATTTTG TGATGGAGAG AGGAAAGCAC 
ATTGGGGCCC GAAGTGTTCT GGATGATACC AGGTTGCTGC ATTGCTTGCA TGCTGCCAAA 
TTACAGAGTG AACAGGAGTA TAAGAAAGGA TCCCAAGGAG TGTGGTCTCA GTACTACCTG 
CCCATGGACA TGGTGAATCT TGTCCATGCA AGGAAAGCCC AGGCCTTAGC AAGTGATCAA 
GAATATAGAA AGAGACTCCA TGAATTTACA GCGCTCCCAG AAGACTTGAA GATGAAATGG 
GCCAAAAGAG CCCACATGCT ACAGAGTGAG CATCGCTATA AATCAGACCT GAACTTCATG 
AAAGGAGTTG GTTGGATGGC TCTGAGATCT CCGCAGATAG AAAGTGTAAA GAAGGCTGGC 
GAGCTCATCA GTGAGACCAA GTATCGTCAG AAGCCAGAAA GTTTGAAGTT CACCGCGGTG 
GTTGACTCTC CAGATTTGAT TCATGCTAAA AATAGCTACC TCCAGTGCAA TGACAGGCTG 
TACAAGGCTG GAGACTCTGA AGCCAGGCAC AGGTATACCC TGCCTCCTGA TCATCCAGAT 
TTTATCCGAG CTCGACAGAA TGCACTTAAT ATCAGTGATA AAGTGTATAA AACATCTTGG 
GAACAGACAA GAGCATCTGG CTATGATTTT AGGATTGACG CCATTCCATT CCAGACAGCA 
AAGGCTTCAA GAGAGATTGC TAGTGATTAC AAATACAGAG AAGCCTTCCT TCGAGACAGA 
GGCCAGAGGA TTGGATTCTT CAGTGCAAAT GATGATGCCC ACACCAGACA TGTCCTCAGA 
GTAGGGAAAC TCCAGAGCGA CAACCTGTAC AGGAGCGGAT ACGCCCAAAA CAGAGGACAT 
TTTCAGAGTC ACCTTAACCA GCCTGGGTTC CTCCATGCTA AGAGAAGCCA GCAGCTTGCC 
AGCAATGTTA ACTACAAGCA GCCCTTGCAC CAATACACTT GTGATCCTGA GCAGCTAAAT 
GTGAAACATG CAAAGCAGGC CTATAAGCTG CAAAGCGATG TAAAATACAA GTCTGACCTG 
AACTGGCTCA GAGGCATTGG CTGGACTCCT CCAGGTTCTT ACAAAGTGGA AAGGGCAAGA 
AGAGCTGCTG AGCTGGCGTA CCTTCGGGAA ATGGGGTTGC AGGCTGCTTC TGCTCAGTAT 
GGACCTGAAG CTGACCAAGT GGGGCCAGAA GGATCACAGC AGGTCACAGT TAATCCTGAT 
GCTTCGGAAA TTCTGCAAGT CAAGAGAAGG AAAATGCAGC TGTACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003641574.1
CDS	193..5403
Translation	MNVQPCARCGYGVYPAEKINCIDQTWHKACFHCEVCKMMLTVNNFVSHEKKPYCQVHNPKNNAFTSIFETPINLNAKKLSEVVSEVKYREESEQFKSAFQWDVRSRDIEAAYKAQHLSSQNAYYAAYEGGTSWYSGNMPDPQMVTVTQAQKNLNDLQYTEEYELRQGKGSFPAMITPGYQVAKRATQLSSNVEYRRGHEERVSKFTSVVDTPDILHAKAGGQLASDLKYTEDFEEQRGKGSFPAMITPAYQIAKRANELASDVKYHQTYEKEIKGKASHTAGTDVTFTRENVDQYGQDYMNEYEERRGKGSFPAMITPAYQNAKKANELASDIKYKKDLSKMKGAAHFHSLTAEDNLVLKQAQSANKLVSEVEYKKDLGNNRGYSVNYCDTPQFKNVSKISKYTSDIKYKETYQNQMKGHYMGIGMDKRMLHAMKVGNLASNIAYKSDYKHDGVDYNYPATLTPSYQTTRKLVPLKDVNYRQSIDKMKYSSVASTPEIAQAKINAQQLSDLNYRAQYEKTKTNYTLPQDIPQLVKAKANAELYSEVKYKEGWEKSKGQGFEMKLDSLPLLAAKASRDLASDIKYKEEYEKTKGKAIETKDSRLLHSLQVAKMSSEIAYKKDFEESKTHFHLPMDMVNLRHAKKAQALASDLDYRKRLHEYTVVPEDLKTKWAKKAYGLQSDLQYREDLMWMKGVGCITEGSLNIQQAKKAGDLVSEKKYRQKVDALKFTSVADSSHIKHAKKSQELQSDVAYRSGKEQFLHQYTITKDDPVFLLAKANAANISEKLYRSSWEKQKEKGFVLRLDALSFLTAKAKRDLASDIKYKEGYEKMKGKLIGVKAVEEDSKMAHSLQMSKLQSDLEYKKAFEDTKNQFQVSMDMANLVHAKKAQNLASDKGYRTALHHYTVLPTDRKVAWAKWAYGLQSDNRYRADLNWMKGAGWIATGSLNVEQAKKAGELISEKKYRQHPYALKFTSIKDTPEMIQARISYNQAVDRLYKEHGESIKHQYTLTADLPEILRAKLNTMNISEIRYKESWQKMKDGGYQLRLDAIPFQAAKASGEIISDHKYKEAFEKMKGQMIGSCGLDGDIRIAHSVHASSLQSDVKYKQGFEDTKTHFHLPLDMINLVHARKAQSLVSEQEYRQLLHQYTSLTDDLRLQCAKNAYKLQSENLYRSDMNFMRGVGCITPGALEIEGKKKASELISESKYRQQPHSFKYTSVTDSPGLLHAKFSNMIANERLYKAAGEDIQHHYTPTLGLPEFTQARINAANLSDVKYRESWHNLCAQGYKLTMEALPFQTARASREIASDYQYKHNFVMERGKHIGARSVLDDTRLLHCLHAAKLQSEQEYKKGSQGVWSQYYLPMDMVNLVHARKAQALASDQEYRKRLHEFTALPEDLKMKWAKRAHMLQSEHRYKSDLNFMKGVGWMALRSPQIESVKKAGELISETKYRQKPESLKFTAVVDSPDLIHAKNSYLQCNDRLYKAGDSEARHRYTLPPDHPDFIRARQNALNISDKVYKTSWEQTRASGYDFRIDAIPFQTAKASREIASDYKYREAFLRDRGQRIGFFSANDDAHTRHVLRVGKLQSDNLYRSGYAQNRGHFQSHLNQPGFLHAKRSQQLASNVNYKQPLHQYTCDPEQLNVKHAKQAYKLQSDVKYKSDLNWLRGIGWTPPGSYKVERARRAAELAYLREMGLQAASAQYGPEADQVGPEGSQQVTVNPDASEILQVKRRKMQLYK

Target ORF information:

RefSeq Version	XM_003641526.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003641526.3

ORF Insert Sequence:

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA GTGAGGTAAA GTATCGAGAG GAGAGTGAGC AATTCAAATC TGCCTTCCAG 
TGGGATGTGA GGTCCAGAGA CATTGAAGCT GCTTATAAAG CCCAACATCT GTCCAGCCAG 
AATGCTTACT ATGCTGCCTA TGAGGGGGGA ACGTCATGGT ACTCAGGAAA CATGCCAGAT 
CCTCAGATGG TCACAGTAAC TCAGGCACAG AAGAATCTGA ATGATCTCCA ATATACAGAA 
GAATATGAGC TGCGTCAAGG CAAAGGCAGC TTTCCTGCTA TGATCACTCC AGGTTATCAG 
GTGGCTAAGA GAGCCACTCA ACTAAGCAGT AATGTGGAAT ATAGGCGGGG ACATGAAGAG 
AGAGTTTCAA AGTTCACCTC TGTTGTAGAC ACTCCAGATA TACTTCACGC AAAGGCTGGA 
GGGCAGCTTG CAAGTGATTT GAAATACACT GAAGACTTTG AAGAACAGAG AGGGAAAGGC 
AGCTTTCCTG CTATGATCAC TCCAGCTTAT CAAATTGCCA AGAGAGCAAA TGAATTAGCC 
AGTGATGTAA AGTATCATCA AACGTACGAA AAAGAAATCA AGGGAAAAGC CAGCCATACA 
GCTGGAACAG ATGTTACTTT TACAAGAGAA AACGTAGATC AATATGGCCA GGATTATATG 
AATGAGTATG AAGAACGCAG AGGGAAAGGA AGCTTTCCTG CTATGATCAC TCCAGCTTAT 
CAAAATGCCA AGAAGGCAAA TGAATTGGCA AGTGATATAA AATACAAGAA AGATCTTTCC 
AAGATGAAAG GTGCAGCTCA CTTTCACTCT CTAACAGCTG AAGACAACCT GGTCCTTAAA 
CAAGCCCAGA GTGCTAACAA GCTTGTTAGT GAGGTGGAAT ACAAGAAGGA TCTTGGAAAC 
AACAGAGGCT ACAGCGTTAA CTACTGTGAT ACTCCTCAAT TCAAGAATGT GAGCAAGATC 
TCAAAATACA CCAGTGATAT TAAGTACAAA GAAACGTACC AGAACCAAAT GAAAGGCCAT 
TATATGGGTA TTGGTATGGA CAAGCGAATG TTACATGCCA TGAAAGTGGG CAACTTGGCA 
AGCAATATAG CCTATAAATC TGATTACAAA CATGACGGTG TTGACTACAA TTACCCAGCT 
ACTCTCACTC CTTCATATCA GACAACAAGG AAGTTGGTCC CTTTGAAAGA TGTAAACTAC 
AGGCAAAGCA TAGACAAGAT GAAGTATAGT TCTGTTGCCA GCACTCCAGA AATTGCTCAA 
GCCAAAATAA ATGCTCAGCA GCTCAGTGAT TTGAACTACC GAGCCCAGTA TGAGAAGACA 
AAAACAAATT ACACTCTACC TCAGGACATA CCTCAGTTAG TCAAGGCAAA AGCCAATGCA 
GAATTATATA GTGAGGTTAA GTACAAAGAA GGCTGGGAGA AGAGCAAGGG CCAGGGATTT 
GAAATGAAGC TTGATTCTCT GCCTTTACTT GCTGCCAAAG CTTCAAGGGA TCTTGCCAGT 
GATATTAAGT ATAAAGAAGA ATATGAAAAA ACAAAAGGGA AAGCAATTGA AACAAAAGAT 
TCAAGACTTT TGCACTCTCT GCAGGTGGCC AAGATGAGTA GTGAGATTGC CTACAAAAAA 
GATTTTGAGG AGAGTAAGAC CCATTTCCAT CTGCCTATGG ACATGGTAAA CCTGAGGCAT 
GCTAAGAAGG CCCAAGCACT AGCTAGCGAC TTGGATTATA GAAAGAGACT CCATGAATAC 
ACAGTGGTTC CGGAAGATTT GAAGACAAAA TGGGCCAAGA AGGCCTATGG GCTCCAGAGT 
GATCTTCAAT ACAGGGAAGA CCTGATGTGG ATGAAAGGAG TTGGATGTAT CACAGAAGGA 
AGTCTTAACA TACAGCAAGC CAAAAAGGCA GGAGATTTGG TCAGTGAGAA AAAATACCGA 
CAGAAGGTTG ATGCTCTGAA ATTCACCAGT GTGGCTGACA GCTCTCACAT CAAACATGCC 
AAGAAAAGCC AGGAACTGCA AAGTGATGTT GCCTACAGGT CAGGAAAAGA GCAGTTTCTT 
CATCAGTACA CCATCACCAA AGATGACCCT GTCTTCTTAC TGGCCAAAGC AAATGCTGCC 
AATATCAGTG AGAAACTGTA CAGAAGTAGC TGGGAGAAGC AGAAGGAAAA AGGATTTGTT 
CTTCGTCTGG ATGCTTTGTC ATTCTTGACA GCTAAGGCAA AGAGGGATCT GGCAAGTGAT 
ATTAAATATA AGGAAGGTTA TGAGAAGATG AAGGGTAAGC TGATTGGAGT AAAAGCTGTG 
GAGGAAGATT CAAAGATGGC TCATTCCCTT CAAATGTCAA AACTGCAGAG CGATCTGGAG 
TACAAGAAGG CATTTGAGGA CACAAAGAAT CAGTTCCAGG TCTCCATGGA CATGGCTAAC 
CTTGTCCATG CCAAAAAGGC TCAGAATTTG GCTTCAGATA AAGGATACAG GACAGCCCTC 
CATCACTACA CTGTCTTGCC TACTGACAGG AAAGTGGCAT GGGCCAAATG GGCGTATGGA 
TTGCAAAGTG ATAACCGATA CAGAGCAGAT TTGAACTGGA TGAAAGGAGC TGGATGGATA 
GCCACTGGGT CATTAAATGT GGAGCAGGCT AAGAAGGCAG GAGAACTCAT TAGTGAGAAG 
AAGTACCGTC AGCATCCATA TGCTTTGAAG TTTACCAGTA TTAAAGACAC TCCTGAGATG 
ATCCAGGCTA GAATTAGTTA TAACCAGGCT GTGGATCGGT TGTACAAGGA GCATGGAGAA 
AGCATAAAGC ATCAGTACAC ACTAACAGCA GATCTTCCTG AAATCCTTCG GGCTAAATTA 
AACACCATGA ATATCAGTGA GATTCGCTAT AAGGAGTCCT GGCAAAAGAT GAAGGATGGT 
GGATATCAGC TGAGACTCGA TGCCATTCCC TTCCAGGCAG CTAAGGCTTC GGGTGAAATC 
ATAAGCGATC ACAAGTATAA GGAGGCATTT GAGAAAATGA AAGGGCAGAT GATTGGTTCA 
TGTGGCCTGG ATGGTGATAT TCGTATTGCT CATTCAGTCC ATGCGTCATC ACTACAGAGT 
GATGTGAAAT ATAAGCAAGG TTTTGAGGAT ACAAAGACTC ACTTCCATCT GCCACTCGAT 
ATGATAAACT TGGTGCATGC CAGGAAAGCC CAGTCTTTGG TCAGTGAACA AGAATACAGA 
CAGCTGCTAC ACCAGTACAC TTCTTTGACT GATGATCTGA GATTGCAGTG TGCCAAGAAT 
GCATATAAAC TACAGAGTGA GAATTTATAT CGGTCTGACA TGAACTTTAT GCGAGGAGTC 
GGATGTATTA CTCCAGGGGC CCTAGAGATT GAAGGAAAGA AGAAAGCCTC TGAACTCATC 
AGTGAGAGTA AATATCGTCA GCAGCCACAT TCCTTCAAGT ACACATCGGT GACAGACTCT 
CCTGGTCTCC TTCATGCAAA ATTCAGCAAT ATGATTGCTA ATGAGCGCCT TTATAAAGCA 
GCGGGAGAGG ACATTCAGCA TCACTATACA CCAACACTGG GTCTGCCTGA GTTCACACAA 
GCAAGAATAA ACGCAGCCAA CCTCAGTGAT GTGAAATACA GGGAATCTTG GCATAATCTA 
TGTGCTCAAG GCTACAAGCT GACAATGGAA GCACTCCCTT TCCAGACTGC GAGGGCCTCC 
AGAGAAATTG CCAGTGATTA CCAGTATAAA CACAATTTTG TGATGGAGAG AGGAAAGCAC 
ATTGGGGCCC GAAGTGTTCT GGATGATACC AGGTTGCTGC ATTGCTTGCA TGCTGCCAAA 
TTACAGAGTG AACAGGAGTA TAAGAAAGGA TCCCAAGGAG TGTGGTCTCA GTACTACCTG 
CCCATGGACA TGGTGAATCT TGTCCATGCA AGGAAAGCCC AGGCCTTAGC AAGTGATCAA 
GAATATAGAA AGAGACTCCA TGAATTTACA GCGCTCCCAG AAGACTTGAA GATGAAATGG 
GCCAAAAGAG CCCACATGCT ACAGAGTGAG CATCGCTATA AATCAGACCT GAACTTCATG 
AAAGGAGTTG GTTGGATGGC TCTGAGATCT CCGCAGATAG AAAGTGTAAA GAAGGCTGGC 
GAGCTCATCA GTGAGACCAA GTATCGTCAG AAGCCAGAAA GTTTGAAGTT CACCGCGGTG 
GTTGACTCTC CAGATTTGAT TCATGCTAAA AATAGCTACC TCCAGTGCAA TGACAGGCTG 
TACAAGGCTG GAGACTCTGA AGCCAGGCAC AGGTATACCC TGCCTCCTGA TCATCCAGAT 
TTTATCCGAG CTCGACAGAA TGCACTTAAT ATCAGTGATA AAGTGTATAA AACATCTTGG 
GAACAGACAA GAGCATCTGG CTATGATTTT AGGATTGACG CCATTCCATT CCAGACAGCA 
AAGGCTTCAA GAGAGATTGC TAGTGATTAC AAATACAGAG AAGCCTTCCT TCGAGACAGA 
GGCCAGAGGA TTGGATTCTT CAGTGCAAAT GATGATGCCC ACACCAGACA TGTCCTCAGA 
GTAGGGAAAC TCCAGAGCGA CAACCTGTAC AGGAGCGGAT ACGCCCAAAA CAGAGGACAT 
TTTCAGAGTC ACCTTAACCA GCCTGGGTTC CTCCATGCTA AGAGAAGCCA GCAGCTTGCC 
AGCAATGTTA ACTACAAGCA GCCCTTGCAC CAATACACTT GTGATCCTGA GCAGCTAAAT 
GTGAAACATG CAAAGCAGGC CTATAAGCTG CAAAGCGATG TAAAATACAA GTCTGACCTG 
AACTGGCTCA GAGGCATTGG CTGGACTCCT CCAGGTTCTT ACAAAGTGGA AAGGGCAAGA 
AGAGCTGCTG AGCTGGCGTA CCTTCGGGAA ATGGGGTTGC AGGCTGCTTC TGCTCAGTAT 
GGACCTGAAG CTGACCAAGT GGGGCCAGAA GGATCACAGC AGGTCACAGT TAATCCTGAT 
GCTTCGGAAA TTCTGCAAGT CAAGAGAAGG AAAATGCAGC TGTACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_004942280.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004942337.1 Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X2, mRNA.

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CloneID	OGa10475
Clone ID Related Accession (Same CDS sequence)	XM_004942280.3 , XM_004942280.2
Accession Version	XM_004942280.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	nebulin-related-anchoring protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004942280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA AGTATCGAGA GGAGAGTGAG CAATTCAAAT CTGCCTTCCA GTGGGATGTG 
AGGTCCAGAG ACATTGAAGC TGCTTATAAA GCCCAACATC TGTCCAGCCA GAATGCTTAC 
TATGCTGCCT ATGAGGGGGG AACGTCATGG TACTCAGGAA ACATGCCAGA TCCTCAGATG 
GTCACAGTAA CTCAGGCACA GAAGAATCTG AATGATCTCC AATATACAGA AGAATATGAG 
CTGCGTCAAG GCAAAGGCAG CTTTCCTGCT ATGATCACTC CAGGTTATCA GGTGGCTAAG 
AGAGCCACTC AACTAAGCAG TAATGTGGAA TATAGGCGGG GACATGAAGA GAGAGTTTCA 
AAGTTCACCT CTGTTGTAGA CACTCCAGAT ATACTTCACG CAAAGGCTGG AGGGCAGCTT 
GCAAGTGATT TGAAATACAC TGAAGACTTT GAAGAACAGA GAGGGAAAGG CAGCTTTCCT 
GCTATGATCA CTCCAGCTTA TCAAATTGCC AAGAGAGCAA ATGAATTAGC CAGTGATGTA 
AAGTATCATC AAACGTACGA AAAAGAAATC AAGGGAAAAG CCAGCCATAC AGCTGGAACA 
GATGTTACTT TTACAAGAGA AAACGTAGAT CAATATGGCC AGGATTATAT GAATGAGTAT 
GAAGAACGCA GAGGGAAAGG AAGCTTTCCT GCTATGATCA CTCCAGCTTA TCAAAATGCC 
AAGAAGGCAA ATGAATTGGC AAGTGATATA AAATACAAGA AAGATCTTTC CAAGATGAAA 
GGTGCAGCTC ACTTTCACTC TCTAACAGCT GAAGACAACC TGGTCCTTAA ACAAGCCCAG 
AGTGCTAACA AGCTTGTTAG TGAGGTGGAA TACAAGAAGG ATCTTGGAAA CAACAGAGGC 
TACAGCGTTA ACTACTGTGA TACTCCTCAA TTCAAGAATG TGAGCAAGAT CTCAAAATAC 
ACCAGTGATA TTAAGTACAA AGAAACGTAC CAGAACCAAA TGAAAGGCCA TTATATGGGT 
ATTGGTATGG ACAAGCGAAT GTTACATGCC ATGAAAGTGG GCAACTTGGC AAGCAATATA 
GCCTATAAAT CTGATTACAA ACATGACGGT GTTGACTACA ATTACCCAGC TACTCTCACT 
CCTTCATATC AGACAACAAG GAAGTTGGTC CCTTTGAAAG ATGTAAACTA CAGGCAAAGC 
ATAGACAAGA TGAAGTATAG TTCTGTTGCC AGCACTCCAG AAATTGCTCA AGCCAAAATA 
AATGCTCAGC AGCTCAGTGA TTTGAACTAC CGAGCCCAGT ATGAGAAGAC AAAAACAAAT 
TACACTCTAC CTCAGGACAT ACCTCAGTTA GTCAAGGCAA AAGCCAATGC AGAATTATAT 
AGTGAGGTTA AGTACAAAGA AGGCTGGGAG AAGAGCAAGG GCCAGGGATT TGAAATGAAG 
CTTGATTCTC TGCCTTTACT TGCTGCCAAA GCTTCAAGGG ATCTTGCCAG TGATATTAAG 
TATAAAGAAG AATATGAAAA AACAAAAGGG AAAGCAATTG AAACAAAAGA TTCAAGACTT 
TTGCACTCTC TGCAGGTGGC CAAGATGAGT AGTGAGATTG CCTACAAAAA AGATTTTGAG 
GAGAGTAAGA CCCATTTCCA TCTGCCTATG GACATGGTAA ACCTGAGGCA TGCTAAGAAG 
GCCCAAGCAC TAGCTAGCGA CTTGGATTAT AGAAAGAGAC TCCATGAATA CACAGTGGTT 
CCGGAAGATT TGAAGACAAA ATGGGCCAAG AAGGCCTATG GGCTCCAGAG TGATCTTCAA 
TACAGGGAAG ACCTGATGTG GATGAAAGGA GTTGGATGTA TCACAGAAGG AAGTCTTAAC 
ATACAGCAAG CCAAAAAGGC AGGAGATTTG GTCAGTGAGA AAAAATACCG ACAGAAGGTT 
GATGCTCTGA AATTCACCAG TGTGGCTGAC AGCTCTCACA TCAAACATGC CAAGAAAAGC 
CAGGAACTGC AAAGTGATGT TGCCTACAGG TCAGGAAAAG AGCAGTTTCT TCATCAGTAC 
ACCATCACCA AAGATGACCC TGTCTTCTTA CTGGCCAAAG CAAATGCTGC CAATATCAGT 
GAGAAACTGT ACAGAAGTAG CTGGGAGAAG CAGAAGGAAA AAGGATTTGT TCTTCGTCTG 
GATGCTTTGT CATTCTTGAC AGCTAAGGCA AAGAGGGATC TGGCAAGTGA TATTAAATAT 
AAGGAAGGTT ATGAGAAGAT GAAGGGTAAG CTGATTGGAG TAAAAGCTGT GGAGGAAGAT 
TCAAAGATGG CTCATTCCCT TCAAATGTCA AAACTGCAGA GCGATCTGGA GTACAAGAAG 
GCATTTGAGG ACACAAAGAA TCAGTTCCAG GTCTCCATGG ACATGGCTAA CCTTGTCCAT 
GCCAAAAAGG CTCAGAATTT GGCTTCAGAT AAAGGATACA GGACAGCCCT CCATCACTAC 
ACTGTCTTGC CTACTGACAG GAAAGTGGCA TGGGCCAAAT GGGCGTATGG ATTGCAAAGT 
GATAACCGAT ACAGAGCAGA TTTGAACTGG ATGAAAGGAG CTGGATGGAT AGCCACTGGG 
TCATTAAATG TGGAGCAGGC TAAGAAGGCA GGAGAACTCA TTAGTGAGAA GAAGTACCGT 
CAGCATCCAT ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCTGAGAT GATCCAGGCT 
AGAATTAGTT ATAACCAGGC TGTGGATCGG TTGTACAAGG AGCATGGAGA AAGCATAAAG 
CATCAGTACA CACTAACAGC AGATCTTCCT GAAATCCTTC GGGCTAAATT AAACACCATG 
AATATCAGTG AGATTCGCTA TAAGGAGTCC TGGCAAAAGA TGAAGGATGG TGGATATCAG 
CTGAGACTCG ATGCCATTCC CTTCCAGGCA GCTAAGGCTT CGGGTGAAAT CATAAGCGAT 
CACAAGTATA AGGAGGCATT TGAGAAAATG AAAGGGCAGA TGATTGGTTC ATGTGGCCTG 
GATGGTGATA TTCGTATTGC TCATTCAGTC CATGCGTCAT CACTACAGAG TGATGTGAAA 
TATAAGCAAG GTTTTGAGGA TACAAAGACT CACTTCCATC TGCCACTCGA TATGATAAAC 
TTGGTGCATG CCAGGAAAGC CCAGTCTTTG GTCAGTGAAC AAGAATACAG ACAGCTGCTA 
CACCAGTACA CTTCTTTGAC TGATGATCTG AGATTGCAGT GTGCCAAGAA TGCATATAAA 
CTACAGAGTG AGAATTTATA TCGGTCTGAC ATGAACTTTA TGCGAGGAGT CGGATGTATT 
ACTCCAGGGG CCCTAGAGAT TGAAGGAAAG AAGAAAGCCT CTGAACTCAT CAGTGAGAGT 
AAATATCGTC AGCAGCCACA TTCCTTCAAG TACACATCGG TGACAGACTC TCCTGGTCTC 
CTTCATGCAA AATTCAGCAA TATGATTGCT AATGAGCGCC TTTATAAAGC AGCGGGAGAG 
GACATTCAGC ATCACTATAC ACCAACACTG GGTCTGCCTG AGTTCACACA AGCAAGAATA 
AACGCAGCCA ACCTCAGTGA TGTGAAATAC AGGGAATCTT GGCATAATCT ATGTGCTCAA 
GGCTACAAGC TGACAATGGA AGCACTCCCT TTCCAGACTG CGAGGGCCTC CAGAGAAATT 
GCCAGTGATT ACCAGTATAA ACACAATTTT GTGATGGAGA GAGGAAAGCA CATTGGGGCC 
CGAAGTGTTC TGGATGATAC CAGGTTGCTG CATTGCTTGC ATGCTGCCAA ATTACAGAGT 
GAACAGGAGT ATAAGAAAGG ATCCCAAGGA GTGTGGTCTC AGTACTACCT GCCCATGGAC 
ATGGTGAATC TTGTCCATGC AAGGAAAGCC CAGGCCTTAG CAAGTGATCA AGAATATAGA 
AAGAGACTCC ATGAATTTAC AGCGCTCCCA GAAGACTTGA AGATGAAATG GGCCAAAAGA 
GCCCACATGC TACAGAGTGA GCATCGCTAT AAATCAGACC TGAACTTCAT GAAAGGAGTT 
GGTTGGATGG CTCTGAGATC TCCGCAGATA GAAAGTGTAA AGAAGGCTGG CGAGCTCATC 
AGTGAGACCA AGTATCGTCA GAAGCCAGAA AGTTTGAAGT TCACCGCGGT GGTTGACTCT 
CCAGATTTGA TTCATGCTAA AAATAGCTAC CTCCAGTGCA ATGACAGGCT GTACAAGGCT 
GGAGACTCTG AAGCCAGGCA CAGGTATACC CTGCCTCCTG ATCATCCAGA TTTTATCCGA 
GCTCGACAGA ATGCACTTAA TATCAGTGAT AAAGTGTATA AAACATCTTG GGAACAGACA 
AGAGCATCTG GCTATGATTT TAGGATTGAC GCCATTCCAT TCCAGACAGC AAAGGCTTCA 
AGAGAGATTG CTAGTGATTA CAAATACAGA GAAGCCTTCC TTCGAGACAG AGGCCAGAGG 
ATTGGATTCT TCAGTGCAAA TGATGATGCC CACACCAGAC ATGTCCTCAG AGTAGGGAAA 
CTCCAGAGCG ACAACCTGTA CAGGAGCGGA TACGCCCAAA ACAGAGGACA TTTTCAGAGT 
CACCTTAACC AGCCTGGGTT CCTCCATGCT AAGAGAAGCC AGCAGCTTGC CAGCAATGTT 
AACTACAAGC AGCCCTTGCA CCAATACACT TGTGATCCTG AGCAGCTAAA TGTGAAACAT 
GCAAAGCAGG CCTATAAGCT GCAAAGCGAT GTAAAATACA AGTCTGACCT GAACTGGCTC 
AGAGGCATTG GCTGGACTCC TCCAGGTTCT TACAAAGTGG AAAGGGCAAG AAGAGCTGCT 
GAGCTGGCGT ACCTTCGGGA AATGGGGTTG CAGGCTGCTT CTGCTCAGTA TGGACCTGAA 
GCTGACCAAG TGGGGCCAGA AGGATCACAG CAGGTCACAG TTAATCCTGA TGCTTCGGAA 
ATTCTGCAAG TCAAGAGAAG GAAAATGCAG CTGTACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004942337.1
CDS	198..5399
Translation	MNVQPCARCGYGVYPAEKINCIDQTWHKACFHCEVCKMMLTVNNFVSHEKKPYCQVHNPKNNAFTSIFETPINLNAKKLSEVVKYREESEQFKSAFQWDVRSRDIEAAYKAQHLSSQNAYYAAYEGGTSWYSGNMPDPQMVTVTQAQKNLNDLQYTEEYELRQGKGSFPAMITPGYQVAKRATQLSSNVEYRRGHEERVSKFTSVVDTPDILHAKAGGQLASDLKYTEDFEEQRGKGSFPAMITPAYQIAKRANELASDVKYHQTYEKEIKGKASHTAGTDVTFTRENVDQYGQDYMNEYEERRGKGSFPAMITPAYQNAKKANELASDIKYKKDLSKMKGAAHFHSLTAEDNLVLKQAQSANKLVSEVEYKKDLGNNRGYSVNYCDTPQFKNVSKISKYTSDIKYKETYQNQMKGHYMGIGMDKRMLHAMKVGNLASNIAYKSDYKHDGVDYNYPATLTPSYQTTRKLVPLKDVNYRQSIDKMKYSSVASTPEIAQAKINAQQLSDLNYRAQYEKTKTNYTLPQDIPQLVKAKANAELYSEVKYKEGWEKSKGQGFEMKLDSLPLLAAKASRDLASDIKYKEEYEKTKGKAIETKDSRLLHSLQVAKMSSEIAYKKDFEESKTHFHLPMDMVNLRHAKKAQALASDLDYRKRLHEYTVVPEDLKTKWAKKAYGLQSDLQYREDLMWMKGVGCITEGSLNIQQAKKAGDLVSEKKYRQKVDALKFTSVADSSHIKHAKKSQELQSDVAYRSGKEQFLHQYTITKDDPVFLLAKANAANISEKLYRSSWEKQKEKGFVLRLDALSFLTAKAKRDLASDIKYKEGYEKMKGKLIGVKAVEEDSKMAHSLQMSKLQSDLEYKKAFEDTKNQFQVSMDMANLVHAKKAQNLASDKGYRTALHHYTVLPTDRKVAWAKWAYGLQSDNRYRADLNWMKGAGWIATGSLNVEQAKKAGELISEKKYRQHPYALKFTSIKDTPEMIQARISYNQAVDRLYKEHGESIKHQYTLTADLPEILRAKLNTMNISEIRYKESWQKMKDGGYQLRLDAIPFQAAKASGEIISDHKYKEAFEKMKGQMIGSCGLDGDIRIAHSVHASSLQSDVKYKQGFEDTKTHFHLPLDMINLVHARKAQSLVSEQEYRQLLHQYTSLTDDLRLQCAKNAYKLQSENLYRSDMNFMRGVGCITPGALEIEGKKKASELISESKYRQQPHSFKYTSVTDSPGLLHAKFSNMIANERLYKAAGEDIQHHYTPTLGLPEFTQARINAANLSDVKYRESWHNLCAQGYKLTMEALPFQTARASREIASDYQYKHNFVMERGKHIGARSVLDDTRLLHCLHAAKLQSEQEYKKGSQGVWSQYYLPMDMVNLVHARKAQALASDQEYRKRLHEFTALPEDLKMKWAKRAHMLQSEHRYKSDLNFMKGVGWMALRSPQIESVKKAGELISETKYRQKPESLKFTAVVDSPDLIHAKNSYLQCNDRLYKAGDSEARHRYTLPPDHPDFIRARQNALNISDKVYKTSWEQTRASGYDFRIDAIPFQTAKASREIASDYKYREAFLRDRGQRIGFFSANDDAHTRHVLRVGKLQSDNLYRSGYAQNRGHFQSHLNQPGFLHAKRSQQLASNVNYKQPLHQYTCDPEQLNVKHAKQAYKLQSDVKYKSDLNWLRGIGWTPPGSYKVERARRAAELAYLREMGLQAASAQYGPEADQVGPEGSQQVTVNPDASEILQVKRRKMQLYK

Target ORF information:

RefSeq Version	XM_004942280.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004942280.3

ORF Insert Sequence:

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA AGTATCGAGA GGAGAGTGAG CAATTCAAAT CTGCCTTCCA GTGGGATGTG 
AGGTCCAGAG ACATTGAAGC TGCTTATAAA GCCCAACATC TGTCCAGCCA GAATGCTTAC 
TATGCTGCCT ATGAGGGGGG AACGTCATGG TACTCAGGAA ACATGCCAGA TCCTCAGATG 
GTCACAGTAA CTCAGGCACA GAAGAATCTG AATGATCTCC AATATACAGA AGAATATGAG 
CTGCGTCAAG GCAAAGGCAG CTTTCCTGCT ATGATCACTC CAGGTTATCA GGTGGCTAAG 
AGAGCCACTC AACTAAGCAG TAATGTGGAA TATAGGCGGG GACATGAAGA GAGAGTTTCA 
AAGTTCACCT CTGTTGTAGA CACTCCAGAT ATACTTCACG CAAAGGCTGG AGGGCAGCTT 
GCAAGTGATT TGAAATACAC TGAAGACTTT GAAGAACAGA GAGGGAAAGG CAGCTTTCCT 
GCTATGATCA CTCCAGCTTA TCAAATTGCC AAGAGAGCAA ATGAATTAGC CAGTGATGTA 
AAGTATCATC AAACGTACGA AAAAGAAATC AAGGGAAAAG CCAGCCATAC AGCTGGAACA 
GATGTTACTT TTACAAGAGA AAACGTAGAT CAATATGGCC AGGATTATAT GAATGAGTAT 
GAAGAACGCA GAGGGAAAGG AAGCTTTCCT GCTATGATCA CTCCAGCTTA TCAAAATGCC 
AAGAAGGCAA ATGAATTGGC AAGTGATATA AAATACAAGA AAGATCTTTC CAAGATGAAA 
GGTGCAGCTC ACTTTCACTC TCTAACAGCT GAAGACAACC TGGTCCTTAA ACAAGCCCAG 
AGTGCTAACA AGCTTGTTAG TGAGGTGGAA TACAAGAAGG ATCTTGGAAA CAACAGAGGC 
TACAGCGTTA ACTACTGTGA TACTCCTCAA TTCAAGAATG TGAGCAAGAT CTCAAAATAC 
ACCAGTGATA TTAAGTACAA AGAAACGTAC CAGAACCAAA TGAAAGGCCA TTATATGGGT 
ATTGGTATGG ACAAGCGAAT GTTACATGCC ATGAAAGTGG GCAACTTGGC AAGCAATATA 
GCCTATAAAT CTGATTACAA ACATGACGGT GTTGACTACA ATTACCCAGC TACTCTCACT 
CCTTCATATC AGACAACAAG GAAGTTGGTC CCTTTGAAAG ATGTAAACTA CAGGCAAAGC 
ATAGACAAGA TGAAGTATAG TTCTGTTGCC AGCACTCCAG AAATTGCTCA AGCCAAAATA 
AATGCTCAGC AGCTCAGTGA TTTGAACTAC CGAGCCCAGT ATGAGAAGAC AAAAACAAAT 
TACACTCTAC CTCAGGACAT ACCTCAGTTA GTCAAGGCAA AAGCCAATGC AGAATTATAT 
AGTGAGGTTA AGTACAAAGA AGGCTGGGAG AAGAGCAAGG GCCAGGGATT TGAAATGAAG 
CTTGATTCTC TGCCTTTACT TGCTGCCAAA GCTTCAAGGG ATCTTGCCAG TGATATTAAG 
TATAAAGAAG AATATGAAAA AACAAAAGGG AAAGCAATTG AAACAAAAGA TTCAAGACTT 
TTGCACTCTC TGCAGGTGGC CAAGATGAGT AGTGAGATTG CCTACAAAAA AGATTTTGAG 
GAGAGTAAGA CCCATTTCCA TCTGCCTATG GACATGGTAA ACCTGAGGCA TGCTAAGAAG 
GCCCAAGCAC TAGCTAGCGA CTTGGATTAT AGAAAGAGAC TCCATGAATA CACAGTGGTT 
CCGGAAGATT TGAAGACAAA ATGGGCCAAG AAGGCCTATG GGCTCCAGAG TGATCTTCAA 
TACAGGGAAG ACCTGATGTG GATGAAAGGA GTTGGATGTA TCACAGAAGG AAGTCTTAAC 
ATACAGCAAG CCAAAAAGGC AGGAGATTTG GTCAGTGAGA AAAAATACCG ACAGAAGGTT 
GATGCTCTGA AATTCACCAG TGTGGCTGAC AGCTCTCACA TCAAACATGC CAAGAAAAGC 
CAGGAACTGC AAAGTGATGT TGCCTACAGG TCAGGAAAAG AGCAGTTTCT TCATCAGTAC 
ACCATCACCA AAGATGACCC TGTCTTCTTA CTGGCCAAAG CAAATGCTGC CAATATCAGT 
GAGAAACTGT ACAGAAGTAG CTGGGAGAAG CAGAAGGAAA AAGGATTTGT TCTTCGTCTG 
GATGCTTTGT CATTCTTGAC AGCTAAGGCA AAGAGGGATC TGGCAAGTGA TATTAAATAT 
AAGGAAGGTT ATGAGAAGAT GAAGGGTAAG CTGATTGGAG TAAAAGCTGT GGAGGAAGAT 
TCAAAGATGG CTCATTCCCT TCAAATGTCA AAACTGCAGA GCGATCTGGA GTACAAGAAG 
GCATTTGAGG ACACAAAGAA TCAGTTCCAG GTCTCCATGG ACATGGCTAA CCTTGTCCAT 
GCCAAAAAGG CTCAGAATTT GGCTTCAGAT AAAGGATACA GGACAGCCCT CCATCACTAC 
ACTGTCTTGC CTACTGACAG GAAAGTGGCA TGGGCCAAAT GGGCGTATGG ATTGCAAAGT 
GATAACCGAT ACAGAGCAGA TTTGAACTGG ATGAAAGGAG CTGGATGGAT AGCCACTGGG 
TCATTAAATG TGGAGCAGGC TAAGAAGGCA GGAGAACTCA TTAGTGAGAA GAAGTACCGT 
CAGCATCCAT ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCTGAGAT GATCCAGGCT 
AGAATTAGTT ATAACCAGGC TGTGGATCGG TTGTACAAGG AGCATGGAGA AAGCATAAAG 
CATCAGTACA CACTAACAGC AGATCTTCCT GAAATCCTTC GGGCTAAATT AAACACCATG 
AATATCAGTG AGATTCGCTA TAAGGAGTCC TGGCAAAAGA TGAAGGATGG TGGATATCAG 
CTGAGACTCG ATGCCATTCC CTTCCAGGCA GCTAAGGCTT CGGGTGAAAT CATAAGCGAT 
CACAAGTATA AGGAGGCATT TGAGAAAATG AAAGGGCAGA TGATTGGTTC ATGTGGCCTG 
GATGGTGATA TTCGTATTGC TCATTCAGTC CATGCGTCAT CACTACAGAG TGATGTGAAA 
TATAAGCAAG GTTTTGAGGA TACAAAGACT CACTTCCATC TGCCACTCGA TATGATAAAC 
TTGGTGCATG CCAGGAAAGC CCAGTCTTTG GTCAGTGAAC AAGAATACAG ACAGCTGCTA 
CACCAGTACA CTTCTTTGAC TGATGATCTG AGATTGCAGT GTGCCAAGAA TGCATATAAA 
CTACAGAGTG AGAATTTATA TCGGTCTGAC ATGAACTTTA TGCGAGGAGT CGGATGTATT 
ACTCCAGGGG CCCTAGAGAT TGAAGGAAAG AAGAAAGCCT CTGAACTCAT CAGTGAGAGT 
AAATATCGTC AGCAGCCACA TTCCTTCAAG TACACATCGG TGACAGACTC TCCTGGTCTC 
CTTCATGCAA AATTCAGCAA TATGATTGCT AATGAGCGCC TTTATAAAGC AGCGGGAGAG 
GACATTCAGC ATCACTATAC ACCAACACTG GGTCTGCCTG AGTTCACACA AGCAAGAATA 
AACGCAGCCA ACCTCAGTGA TGTGAAATAC AGGGAATCTT GGCATAATCT ATGTGCTCAA 
GGCTACAAGC TGACAATGGA AGCACTCCCT TTCCAGACTG CGAGGGCCTC CAGAGAAATT 
GCCAGTGATT ACCAGTATAA ACACAATTTT GTGATGGAGA GAGGAAAGCA CATTGGGGCC 
CGAAGTGTTC TGGATGATAC CAGGTTGCTG CATTGCTTGC ATGCTGCCAA ATTACAGAGT 
GAACAGGAGT ATAAGAAAGG ATCCCAAGGA GTGTGGTCTC AGTACTACCT GCCCATGGAC 
ATGGTGAATC TTGTCCATGC AAGGAAAGCC CAGGCCTTAG CAAGTGATCA AGAATATAGA 
AAGAGACTCC ATGAATTTAC AGCGCTCCCA GAAGACTTGA AGATGAAATG GGCCAAAAGA 
GCCCACATGC TACAGAGTGA GCATCGCTAT AAATCAGACC TGAACTTCAT GAAAGGAGTT 
GGTTGGATGG CTCTGAGATC TCCGCAGATA GAAAGTGTAA AGAAGGCTGG CGAGCTCATC 
AGTGAGACCA AGTATCGTCA GAAGCCAGAA AGTTTGAAGT TCACCGCGGT GGTTGACTCT 
CCAGATTTGA TTCATGCTAA AAATAGCTAC CTCCAGTGCA ATGACAGGCT GTACAAGGCT 
GGAGACTCTG AAGCCAGGCA CAGGTATACC CTGCCTCCTG ATCATCCAGA TTTTATCCGA 
GCTCGACAGA ATGCACTTAA TATCAGTGAT AAAGTGTATA AAACATCTTG GGAACAGACA 
AGAGCATCTG GCTATGATTT TAGGATTGAC GCCATTCCAT TCCAGACAGC AAAGGCTTCA 
AGAGAGATTG CTAGTGATTA CAAATACAGA GAAGCCTTCC TTCGAGACAG AGGCCAGAGG 
ATTGGATTCT TCAGTGCAAA TGATGATGCC CACACCAGAC ATGTCCTCAG AGTAGGGAAA 
CTCCAGAGCG ACAACCTGTA CAGGAGCGGA TACGCCCAAA ACAGAGGACA TTTTCAGAGT 
CACCTTAACC AGCCTGGGTT CCTCCATGCT AAGAGAAGCC AGCAGCTTGC CAGCAATGTT 
AACTACAAGC AGCCCTTGCA CCAATACACT TGTGATCCTG AGCAGCTAAA TGTGAAACAT 
GCAAAGCAGG CCTATAAGCT GCAAAGCGAT GTAAAATACA AGTCTGACCT GAACTGGCTC 
AGAGGCATTG GCTGGACTCC TCCAGGTTCT TACAAAGTGG AAAGGGCAAG AAGAGCTGCT 
GAGCTGGCGT ACCTTCGGGA AATGGGGTTG CAGGCTGCTT CTGCTCAGTA TGGACCTGAA 
GCTGACCAAG TGGGGCCAGA AGGATCACAG CAGGTCACAG TTAATCCTGA TGCTTCGGAA 
ATTCTGCAAG TCAAGAGAAG GAAAATGCAG CTGTACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_004942280.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004942337.1 Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X2, mRNA.

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CloneID	OGa10475
Clone ID Related Accession (Same CDS sequence)	XM_004942280.3 , XM_004942280.2
Accession Version	XM_004942280.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	nebulin-related-anchoring protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455898.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004942280.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA AGTATCGAGA GGAGAGTGAG CAATTCAAAT CTGCCTTCCA GTGGGATGTG 
AGGTCCAGAG ACATTGAAGC TGCTTATAAA GCCCAACATC TGTCCAGCCA GAATGCTTAC 
TATGCTGCCT ATGAGGGGGG AACGTCATGG TACTCAGGAA ACATGCCAGA TCCTCAGATG 
GTCACAGTAA CTCAGGCACA GAAGAATCTG AATGATCTCC AATATACAGA AGAATATGAG 
CTGCGTCAAG GCAAAGGCAG CTTTCCTGCT ATGATCACTC CAGGTTATCA GGTGGCTAAG 
AGAGCCACTC AACTAAGCAG TAATGTGGAA TATAGGCGGG GACATGAAGA GAGAGTTTCA 
AAGTTCACCT CTGTTGTAGA CACTCCAGAT ATACTTCACG CAAAGGCTGG AGGGCAGCTT 
GCAAGTGATT TGAAATACAC TGAAGACTTT GAAGAACAGA GAGGGAAAGG CAGCTTTCCT 
GCTATGATCA CTCCAGCTTA TCAAATTGCC AAGAGAGCAA ATGAATTAGC CAGTGATGTA 
AAGTATCATC AAACGTACGA AAAAGAAATC AAGGGAAAAG CCAGCCATAC AGCTGGAACA 
GATGTTACTT TTACAAGAGA AAACGTAGAT CAATATGGCC AGGATTATAT GAATGAGTAT 
GAAGAACGCA GAGGGAAAGG AAGCTTTCCT GCTATGATCA CTCCAGCTTA TCAAAATGCC 
AAGAAGGCAA ATGAATTGGC AAGTGATATA AAATACAAGA AAGATCTTTC CAAGATGAAA 
GGTGCAGCTC ACTTTCACTC TCTAACAGCT GAAGACAACC TGGTCCTTAA ACAAGCCCAG 
AGTGCTAACA AGCTTGTTAG TGAGGTGGAA TACAAGAAGG ATCTTGGAAA CAACAGAGGC 
TACAGCGTTA ACTACTGTGA TACTCCTCAA TTCAAGAATG TGAGCAAGAT CTCAAAATAC 
ACCAGTGATA TTAAGTACAA AGAAACGTAC CAGAACCAAA TGAAAGGCCA TTATATGGGT 
ATTGGTATGG ACAAGCGAAT GTTACATGCC ATGAAAGTGG GCAACTTGGC AAGCAATATA 
GCCTATAAAT CTGATTACAA ACATGACGGT GTTGACTACA ATTACCCAGC TACTCTCACT 
CCTTCATATC AGACAACAAG GAAGTTGGTC CCTTTGAAAG ATGTAAACTA CAGGCAAAGC 
ATAGACAAGA TGAAGTATAG TTCTGTTGCC AGCACTCCAG AAATTGCTCA AGCCAAAATA 
AATGCTCAGC AGCTCAGTGA TTTGAACTAC CGAGCCCAGT ATGAGAAGAC AAAAACAAAT 
TACACTCTAC CTCAGGACAT ACCTCAGTTA GTCAAGGCAA AAGCCAATGC AGAATTATAT 
AGTGAGGTTA AGTACAAAGA AGGCTGGGAG AAGAGCAAGG GCCAGGGATT TGAAATGAAG 
CTTGATTCTC TGCCTTTACT TGCTGCCAAA GCTTCAAGGG ATCTTGCCAG TGATATTAAG 
TATAAAGAAG AATATGAAAA AACAAAAGGG AAAGCAATTG AAACAAAAGA TTCAAGACTT 
TTGCACTCTC TGCAGGTGGC CAAGATGAGT AGTGAGATTG CCTACAAAAA AGATTTTGAG 
GAGAGTAAGA CCCATTTCCA TCTGCCTATG GACATGGTAA ACCTGAGGCA TGCTAAGAAG 
GCCCAAGCAC TAGCTAGCGA CTTGGATTAT AGAAAGAGAC TCCATGAATA CACAGTGGTT 
CCGGAAGATT TGAAGACAAA ATGGGCCAAG AAGGCCTATG GGCTCCAGAG TGATCTTCAA 
TACAGGGAAG ACCTGATGTG GATGAAAGGA GTTGGATGTA TCACAGAAGG AAGTCTTAAC 
ATACAGCAAG CCAAAAAGGC AGGAGATTTG GTCAGTGAGA AAAAATACCG ACAGAAGGTT 
GATGCTCTGA AATTCACCAG TGTGGCTGAC AGCTCTCACA TCAAACATGC CAAGAAAAGC 
CAGGAACTGC AAAGTGATGT TGCCTACAGG TCAGGAAAAG AGCAGTTTCT TCATCAGTAC 
ACCATCACCA AAGATGACCC TGTCTTCTTA CTGGCCAAAG CAAATGCTGC CAATATCAGT 
GAGAAACTGT ACAGAAGTAG CTGGGAGAAG CAGAAGGAAA AAGGATTTGT TCTTCGTCTG 
GATGCTTTGT CATTCTTGAC AGCTAAGGCA AAGAGGGATC TGGCAAGTGA TATTAAATAT 
AAGGAAGGTT ATGAGAAGAT GAAGGGTAAG CTGATTGGAG TAAAAGCTGT GGAGGAAGAT 
TCAAAGATGG CTCATTCCCT TCAAATGTCA AAACTGCAGA GCGATCTGGA GTACAAGAAG 
GCATTTGAGG ACACAAAGAA TCAGTTCCAG GTCTCCATGG ACATGGCTAA CCTTGTCCAT 
GCCAAAAAGG CTCAGAATTT GGCTTCAGAT AAAGGATACA GGACAGCCCT CCATCACTAC 
ACTGTCTTGC CTACTGACAG GAAAGTGGCA TGGGCCAAAT GGGCGTATGG ATTGCAAAGT 
GATAACCGAT ACAGAGCAGA TTTGAACTGG ATGAAAGGAG CTGGATGGAT AGCCACTGGG 
TCATTAAATG TGGAGCAGGC TAAGAAGGCA GGAGAACTCA TTAGTGAGAA GAAGTACCGT 
CAGCATCCAT ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCTGAGAT GATCCAGGCT 
AGAATTAGTT ATAACCAGGC TGTGGATCGG TTGTACAAGG AGCATGGAGA AAGCATAAAG 
CATCAGTACA CACTAACAGC AGATCTTCCT GAAATCCTTC GGGCTAAATT AAACACCATG 
AATATCAGTG AGATTCGCTA TAAGGAGTCC TGGCAAAAGA TGAAGGATGG TGGATATCAG 
CTGAGACTCG ATGCCATTCC CTTCCAGGCA GCTAAGGCTT CGGGTGAAAT CATAAGCGAT 
CACAAGTATA AGGAGGCATT TGAGAAAATG AAAGGGCAGA TGATTGGTTC ATGTGGCCTG 
GATGGTGATA TTCGTATTGC TCATTCAGTC CATGCGTCAT CACTACAGAG TGATGTGAAA 
TATAAGCAAG GTTTTGAGGA TACAAAGACT CACTTCCATC TGCCACTCGA TATGATAAAC 
TTGGTGCATG CCAGGAAAGC CCAGTCTTTG GTCAGTGAAC AAGAATACAG ACAGCTGCTA 
CACCAGTACA CTTCTTTGAC TGATGATCTG AGATTGCAGT GTGCCAAGAA TGCATATAAA 
CTACAGAGTG AGAATTTATA TCGGTCTGAC ATGAACTTTA TGCGAGGAGT CGGATGTATT 
ACTCCAGGGG CCCTAGAGAT TGAAGGAAAG AAGAAAGCCT CTGAACTCAT CAGTGAGAGT 
AAATATCGTC AGCAGCCACA TTCCTTCAAG TACACATCGG TGACAGACTC TCCTGGTCTC 
CTTCATGCAA AATTCAGCAA TATGATTGCT AATGAGCGCC TTTATAAAGC AGCGGGAGAG 
GACATTCAGC ATCACTATAC ACCAACACTG GGTCTGCCTG AGTTCACACA AGCAAGAATA 
AACGCAGCCA ACCTCAGTGA TGTGAAATAC AGGGAATCTT GGCATAATCT ATGTGCTCAA 
GGCTACAAGC TGACAATGGA AGCACTCCCT TTCCAGACTG CGAGGGCCTC CAGAGAAATT 
GCCAGTGATT ACCAGTATAA ACACAATTTT GTGATGGAGA GAGGAAAGCA CATTGGGGCC 
CGAAGTGTTC TGGATGATAC CAGGTTGCTG CATTGCTTGC ATGCTGCCAA ATTACAGAGT 
GAACAGGAGT ATAAGAAAGG ATCCCAAGGA GTGTGGTCTC AGTACTACCT GCCCATGGAC 
ATGGTGAATC TTGTCCATGC AAGGAAAGCC CAGGCCTTAG CAAGTGATCA AGAATATAGA 
AAGAGACTCC ATGAATTTAC AGCGCTCCCA GAAGACTTGA AGATGAAATG GGCCAAAAGA 
GCCCACATGC TACAGAGTGA GCATCGCTAT AAATCAGACC TGAACTTCAT GAAAGGAGTT 
GGTTGGATGG CTCTGAGATC TCCGCAGATA GAAAGTGTAA AGAAGGCTGG CGAGCTCATC 
AGTGAGACCA AGTATCGTCA GAAGCCAGAA AGTTTGAAGT TCACCGCGGT GGTTGACTCT 
CCAGATTTGA TTCATGCTAA AAATAGCTAC CTCCAGTGCA ATGACAGGCT GTACAAGGCT 
GGAGACTCTG AAGCCAGGCA CAGGTATACC CTGCCTCCTG ATCATCCAGA TTTTATCCGA 
GCTCGACAGA ATGCACTTAA TATCAGTGAT AAAGTGTATA AAACATCTTG GGAACAGACA 
AGAGCATCTG GCTATGATTT TAGGATTGAC GCCATTCCAT TCCAGACAGC AAAGGCTTCA 
AGAGAGATTG CTAGTGATTA CAAATACAGA GAAGCCTTCC TTCGAGACAG AGGCCAGAGG 
ATTGGATTCT TCAGTGCAAA TGATGATGCC CACACCAGAC ATGTCCTCAG AGTAGGGAAA 
CTCCAGAGCG ACAACCTGTA CAGGAGCGGA TACGCCCAAA ACAGAGGACA TTTTCAGAGT 
CACCTTAACC AGCCTGGGTT CCTCCATGCT AAGAGAAGCC AGCAGCTTGC CAGCAATGTT 
AACTACAAGC AGCCCTTGCA CCAATACACT TGTGATCCTG AGCAGCTAAA TGTGAAACAT 
GCAAAGCAGG CCTATAAGCT GCAAAGCGAT GTAAAATACA AGTCTGACCT GAACTGGCTC 
AGAGGCATTG GCTGGACTCC TCCAGGTTCT TACAAAGTGG AAAGGGCAAG AAGAGCTGCT 
GAGCTGGCGT ACCTTCGGGA AATGGGGTTG CAGGCTGCTT CTGCTCAGTA TGGACCTGAA 
GCTGACCAAG TGGGGCCAGA AGGATCACAG CAGGTCACAG TTAATCCTGA TGCTTCGGAA 
ATTCTGCAAG TCAAGAGAAG GAAAATGCAG CTGTACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004942337.1
CDS	193..5394
Translation	MNVQPCARCGYGVYPAEKINCIDQTWHKACFHCEVCKMMLTVNNFVSHEKKPYCQVHNPKNNAFTSIFETPINLNAKKLSEVVKYREESEQFKSAFQWDVRSRDIEAAYKAQHLSSQNAYYAAYEGGTSWYSGNMPDPQMVTVTQAQKNLNDLQYTEEYELRQGKGSFPAMITPGYQVAKRATQLSSNVEYRRGHEERVSKFTSVVDTPDILHAKAGGQLASDLKYTEDFEEQRGKGSFPAMITPAYQIAKRANELASDVKYHQTYEKEIKGKASHTAGTDVTFTRENVDQYGQDYMNEYEERRGKGSFPAMITPAYQNAKKANELASDIKYKKDLSKMKGAAHFHSLTAEDNLVLKQAQSANKLVSEVEYKKDLGNNRGYSVNYCDTPQFKNVSKISKYTSDIKYKETYQNQMKGHYMGIGMDKRMLHAMKVGNLASNIAYKSDYKHDGVDYNYPATLTPSYQTTRKLVPLKDVNYRQSIDKMKYSSVASTPEIAQAKINAQQLSDLNYRAQYEKTKTNYTLPQDIPQLVKAKANAELYSEVKYKEGWEKSKGQGFEMKLDSLPLLAAKASRDLASDIKYKEEYEKTKGKAIETKDSRLLHSLQVAKMSSEIAYKKDFEESKTHFHLPMDMVNLRHAKKAQALASDLDYRKRLHEYTVVPEDLKTKWAKKAYGLQSDLQYREDLMWMKGVGCITEGSLNIQQAKKAGDLVSEKKYRQKVDALKFTSVADSSHIKHAKKSQELQSDVAYRSGKEQFLHQYTITKDDPVFLLAKANAANISEKLYRSSWEKQKEKGFVLRLDALSFLTAKAKRDLASDIKYKEGYEKMKGKLIGVKAVEEDSKMAHSLQMSKLQSDLEYKKAFEDTKNQFQVSMDMANLVHAKKAQNLASDKGYRTALHHYTVLPTDRKVAWAKWAYGLQSDNRYRADLNWMKGAGWIATGSLNVEQAKKAGELISEKKYRQHPYALKFTSIKDTPEMIQARISYNQAVDRLYKEHGESIKHQYTLTADLPEILRAKLNTMNISEIRYKESWQKMKDGGYQLRLDAIPFQAAKASGEIISDHKYKEAFEKMKGQMIGSCGLDGDIRIAHSVHASSLQSDVKYKQGFEDTKTHFHLPLDMINLVHARKAQSLVSEQEYRQLLHQYTSLTDDLRLQCAKNAYKLQSENLYRSDMNFMRGVGCITPGALEIEGKKKASELISESKYRQQPHSFKYTSVTDSPGLLHAKFSNMIANERLYKAAGEDIQHHYTPTLGLPEFTQARINAANLSDVKYRESWHNLCAQGYKLTMEALPFQTARASREIASDYQYKHNFVMERGKHIGARSVLDDTRLLHCLHAAKLQSEQEYKKGSQGVWSQYYLPMDMVNLVHARKAQALASDQEYRKRLHEFTALPEDLKMKWAKRAHMLQSEHRYKSDLNFMKGVGWMALRSPQIESVKKAGELISETKYRQKPESLKFTAVVDSPDLIHAKNSYLQCNDRLYKAGDSEARHRYTLPPDHPDFIRARQNALNISDKVYKTSWEQTRASGYDFRIDAIPFQTAKASREIASDYKYREAFLRDRGQRIGFFSANDDAHTRHVLRVGKLQSDNLYRSGYAQNRGHFQSHLNQPGFLHAKRSQQLASNVNYKQPLHQYTCDPEQLNVKHAKQAYKLQSDVKYKSDLNWLRGIGWTPPGSYKVERARRAAELAYLREMGLQAASAQYGPEADQVGPEGSQQVTVNPDASEILQVKRRKMQLYK

Target ORF information:

RefSeq Version	XM_004942280.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004942280.2

ORF Insert Sequence:

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA AGTATCGAGA GGAGAGTGAG CAATTCAAAT CTGCCTTCCA GTGGGATGTG 
AGGTCCAGAG ACATTGAAGC TGCTTATAAA GCCCAACATC TGTCCAGCCA GAATGCTTAC 
TATGCTGCCT ATGAGGGGGG AACGTCATGG TACTCAGGAA ACATGCCAGA TCCTCAGATG 
GTCACAGTAA CTCAGGCACA GAAGAATCTG AATGATCTCC AATATACAGA AGAATATGAG 
CTGCGTCAAG GCAAAGGCAG CTTTCCTGCT ATGATCACTC CAGGTTATCA GGTGGCTAAG 
AGAGCCACTC AACTAAGCAG TAATGTGGAA TATAGGCGGG GACATGAAGA GAGAGTTTCA 
AAGTTCACCT CTGTTGTAGA CACTCCAGAT ATACTTCACG CAAAGGCTGG AGGGCAGCTT 
GCAAGTGATT TGAAATACAC TGAAGACTTT GAAGAACAGA GAGGGAAAGG CAGCTTTCCT 
GCTATGATCA CTCCAGCTTA TCAAATTGCC AAGAGAGCAA ATGAATTAGC CAGTGATGTA 
AAGTATCATC AAACGTACGA AAAAGAAATC AAGGGAAAAG CCAGCCATAC AGCTGGAACA 
GATGTTACTT TTACAAGAGA AAACGTAGAT CAATATGGCC AGGATTATAT GAATGAGTAT 
GAAGAACGCA GAGGGAAAGG AAGCTTTCCT GCTATGATCA CTCCAGCTTA TCAAAATGCC 
AAGAAGGCAA ATGAATTGGC AAGTGATATA AAATACAAGA AAGATCTTTC CAAGATGAAA 
GGTGCAGCTC ACTTTCACTC TCTAACAGCT GAAGACAACC TGGTCCTTAA ACAAGCCCAG 
AGTGCTAACA AGCTTGTTAG TGAGGTGGAA TACAAGAAGG ATCTTGGAAA CAACAGAGGC 
TACAGCGTTA ACTACTGTGA TACTCCTCAA TTCAAGAATG TGAGCAAGAT CTCAAAATAC 
ACCAGTGATA TTAAGTACAA AGAAACGTAC CAGAACCAAA TGAAAGGCCA TTATATGGGT 
ATTGGTATGG ACAAGCGAAT GTTACATGCC ATGAAAGTGG GCAACTTGGC AAGCAATATA 
GCCTATAAAT CTGATTACAA ACATGACGGT GTTGACTACA ATTACCCAGC TACTCTCACT 
CCTTCATATC AGACAACAAG GAAGTTGGTC CCTTTGAAAG ATGTAAACTA CAGGCAAAGC 
ATAGACAAGA TGAAGTATAG TTCTGTTGCC AGCACTCCAG AAATTGCTCA AGCCAAAATA 
AATGCTCAGC AGCTCAGTGA TTTGAACTAC CGAGCCCAGT ATGAGAAGAC AAAAACAAAT 
TACACTCTAC CTCAGGACAT ACCTCAGTTA GTCAAGGCAA AAGCCAATGC AGAATTATAT 
AGTGAGGTTA AGTACAAAGA AGGCTGGGAG AAGAGCAAGG GCCAGGGATT TGAAATGAAG 
CTTGATTCTC TGCCTTTACT TGCTGCCAAA GCTTCAAGGG ATCTTGCCAG TGATATTAAG 
TATAAAGAAG AATATGAAAA AACAAAAGGG AAAGCAATTG AAACAAAAGA TTCAAGACTT 
TTGCACTCTC TGCAGGTGGC CAAGATGAGT AGTGAGATTG CCTACAAAAA AGATTTTGAG 
GAGAGTAAGA CCCATTTCCA TCTGCCTATG GACATGGTAA ACCTGAGGCA TGCTAAGAAG 
GCCCAAGCAC TAGCTAGCGA CTTGGATTAT AGAAAGAGAC TCCATGAATA CACAGTGGTT 
CCGGAAGATT TGAAGACAAA ATGGGCCAAG AAGGCCTATG GGCTCCAGAG TGATCTTCAA 
TACAGGGAAG ACCTGATGTG GATGAAAGGA GTTGGATGTA TCACAGAAGG AAGTCTTAAC 
ATACAGCAAG CCAAAAAGGC AGGAGATTTG GTCAGTGAGA AAAAATACCG ACAGAAGGTT 
GATGCTCTGA AATTCACCAG TGTGGCTGAC AGCTCTCACA TCAAACATGC CAAGAAAAGC 
CAGGAACTGC AAAGTGATGT TGCCTACAGG TCAGGAAAAG AGCAGTTTCT TCATCAGTAC 
ACCATCACCA AAGATGACCC TGTCTTCTTA CTGGCCAAAG CAAATGCTGC CAATATCAGT 
GAGAAACTGT ACAGAAGTAG CTGGGAGAAG CAGAAGGAAA AAGGATTTGT TCTTCGTCTG 
GATGCTTTGT CATTCTTGAC AGCTAAGGCA AAGAGGGATC TGGCAAGTGA TATTAAATAT 
AAGGAAGGTT ATGAGAAGAT GAAGGGTAAG CTGATTGGAG TAAAAGCTGT GGAGGAAGAT 
TCAAAGATGG CTCATTCCCT TCAAATGTCA AAACTGCAGA GCGATCTGGA GTACAAGAAG 
GCATTTGAGG ACACAAAGAA TCAGTTCCAG GTCTCCATGG ACATGGCTAA CCTTGTCCAT 
GCCAAAAAGG CTCAGAATTT GGCTTCAGAT AAAGGATACA GGACAGCCCT CCATCACTAC 
ACTGTCTTGC CTACTGACAG GAAAGTGGCA TGGGCCAAAT GGGCGTATGG ATTGCAAAGT 
GATAACCGAT ACAGAGCAGA TTTGAACTGG ATGAAAGGAG CTGGATGGAT AGCCACTGGG 
TCATTAAATG TGGAGCAGGC TAAGAAGGCA GGAGAACTCA TTAGTGAGAA GAAGTACCGT 
CAGCATCCAT ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCTGAGAT GATCCAGGCT 
AGAATTAGTT ATAACCAGGC TGTGGATCGG TTGTACAAGG AGCATGGAGA AAGCATAAAG 
CATCAGTACA CACTAACAGC AGATCTTCCT GAAATCCTTC GGGCTAAATT AAACACCATG 
AATATCAGTG AGATTCGCTA TAAGGAGTCC TGGCAAAAGA TGAAGGATGG TGGATATCAG 
CTGAGACTCG ATGCCATTCC CTTCCAGGCA GCTAAGGCTT CGGGTGAAAT CATAAGCGAT 
CACAAGTATA AGGAGGCATT TGAGAAAATG AAAGGGCAGA TGATTGGTTC ATGTGGCCTG 
GATGGTGATA TTCGTATTGC TCATTCAGTC CATGCGTCAT CACTACAGAG TGATGTGAAA 
TATAAGCAAG GTTTTGAGGA TACAAAGACT CACTTCCATC TGCCACTCGA TATGATAAAC 
TTGGTGCATG CCAGGAAAGC CCAGTCTTTG GTCAGTGAAC AAGAATACAG ACAGCTGCTA 
CACCAGTACA CTTCTTTGAC TGATGATCTG AGATTGCAGT GTGCCAAGAA TGCATATAAA 
CTACAGAGTG AGAATTTATA TCGGTCTGAC ATGAACTTTA TGCGAGGAGT CGGATGTATT 
ACTCCAGGGG CCCTAGAGAT TGAAGGAAAG AAGAAAGCCT CTGAACTCAT CAGTGAGAGT 
AAATATCGTC AGCAGCCACA TTCCTTCAAG TACACATCGG TGACAGACTC TCCTGGTCTC 
CTTCATGCAA AATTCAGCAA TATGATTGCT AATGAGCGCC TTTATAAAGC AGCGGGAGAG 
GACATTCAGC ATCACTATAC ACCAACACTG GGTCTGCCTG AGTTCACACA AGCAAGAATA 
AACGCAGCCA ACCTCAGTGA TGTGAAATAC AGGGAATCTT GGCATAATCT ATGTGCTCAA 
GGCTACAAGC TGACAATGGA AGCACTCCCT TTCCAGACTG CGAGGGCCTC CAGAGAAATT 
GCCAGTGATT ACCAGTATAA ACACAATTTT GTGATGGAGA GAGGAAAGCA CATTGGGGCC 
CGAAGTGTTC TGGATGATAC CAGGTTGCTG CATTGCTTGC ATGCTGCCAA ATTACAGAGT 
GAACAGGAGT ATAAGAAAGG ATCCCAAGGA GTGTGGTCTC AGTACTACCT GCCCATGGAC 
ATGGTGAATC TTGTCCATGC AAGGAAAGCC CAGGCCTTAG CAAGTGATCA AGAATATAGA 
AAGAGACTCC ATGAATTTAC AGCGCTCCCA GAAGACTTGA AGATGAAATG GGCCAAAAGA 
GCCCACATGC TACAGAGTGA GCATCGCTAT AAATCAGACC TGAACTTCAT GAAAGGAGTT 
GGTTGGATGG CTCTGAGATC TCCGCAGATA GAAAGTGTAA AGAAGGCTGG CGAGCTCATC 
AGTGAGACCA AGTATCGTCA GAAGCCAGAA AGTTTGAAGT TCACCGCGGT GGTTGACTCT 
CCAGATTTGA TTCATGCTAA AAATAGCTAC CTCCAGTGCA ATGACAGGCT GTACAAGGCT 
GGAGACTCTG AAGCCAGGCA CAGGTATACC CTGCCTCCTG ATCATCCAGA TTTTATCCGA 
GCTCGACAGA ATGCACTTAA TATCAGTGAT AAAGTGTATA AAACATCTTG GGAACAGACA 
AGAGCATCTG GCTATGATTT TAGGATTGAC GCCATTCCAT TCCAGACAGC AAAGGCTTCA 
AGAGAGATTG CTAGTGATTA CAAATACAGA GAAGCCTTCC TTCGAGACAG AGGCCAGAGG 
ATTGGATTCT TCAGTGCAAA TGATGATGCC CACACCAGAC ATGTCCTCAG AGTAGGGAAA 
CTCCAGAGCG ACAACCTGTA CAGGAGCGGA TACGCCCAAA ACAGAGGACA TTTTCAGAGT 
CACCTTAACC AGCCTGGGTT CCTCCATGCT AAGAGAAGCC AGCAGCTTGC CAGCAATGTT 
AACTACAAGC AGCCCTTGCA CCAATACACT TGTGATCCTG AGCAGCTAAA TGTGAAACAT 
GCAAAGCAGG CCTATAAGCT GCAAAGCGAT GTAAAATACA AGTCTGACCT GAACTGGCTC 
AGAGGCATTG GCTGGACTCC TCCAGGTTCT TACAAAGTGG AAAGGGCAAG AAGAGCTGCT 
GAGCTGGCGT ACCTTCGGGA AATGGGGTTG CAGGCTGCTT CTGCTCAGTA TGGACCTGAA 
GCTGACCAAG TGGGGCCAGA AGGATCACAG CAGGTCACAG TTAATCCTGA TGCTTCGGAA 
ATTCTGCAAG TCAAGAGAAG GAAAATGCAG CTGTACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_015288962.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144448.1 Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X3, mRNA.

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CloneID	OGa38032
Clone ID Related Accession (Same CDS sequence)	XM_015288962.1 , XM_015288962.2
Accession Version	XM_015288962.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	nebulin-related-anchoring protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455898.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA GTGAGGTAAA GTATCGAGAG GAGAGTGAGC AATTCAAATC TGCCTTCCAG 
TGGGATGTGA GGTCCAGAGA CATTGAAGCT GCTTATAAAG CCCAACATCT GTCCAGCCAG 
AATGCTTACT ATGCTGCCTA TGAGGGGGGA ACGTCATGGT ACTCAGGAAA CATGCCAGAT 
CCTCAGATGG TCACAGTAAC TCAGGCACAG AAGAATCTGA ATGATCTCCA ATATACAGAA 
GAATATGAGC TGCGTCAAGG CAAAGGCAGC TTTCCTGCTA TGATCACTCC AGGTTATCAG 
GTGGCTAAGA GAGCCACTCA ACTAAGCAGT AATGTGGAAT ATAGGCGGGG ACATGAAGAG 
AGAGTTTCAA AGTTCACCTC TGTTGTAGAC ACTCCAGATA TACTTCACGC AAAGGCTGGA 
GGGCAGCTTG CAAGTGATTT GAAATACACT GAAGACTTTG AAGAACAGAG AGGGAAAGGC 
AGCTTTCCTG CTATGATCAC TCCAGCTTAT CAAATTGCCA AGAGAGCAAA TGAATTAGCC 
AGTGATGTAA AGTATCATCA AACGTACGAA AAAGAAATCA AGGGAAAAGC CAGCCATACA 
GCTGGAACAG ATGTTACTTT TACAAGAGAA AACGTAGATC AATATGGCCA GGATTATATG 
AATGAGTATG AAGAACGCAG AGGGAAAGGA AGCTTTCCTG CTATGATCAC TCCAGCTTAT 
CAAAATGCCA AGAAGGCAAA TGAATTGGCA AGTGATATAA AATACAAGAA AGATCTTTCC 
AAGATGAAAG GTGCAGCTCA CTTTCACTCT CTAACAGCTG AAGACAACCT GGTCCTTAAA 
CAAGCCCAGA GTGCTAACAA GCTTGTTAGT GAGGTGGAAT ACAAGAAGGA TCTTGGAAAC 
AACAGAGGCT ACAGCGTTAA CTACTGTGAT ACTCCTCAAT TCAAGAATGT GAGCAAGATC 
TCAAAATACA CCAGTGATAT TAAGTACAAA GAAACGTACC AGAACCAAAT GAAAGGCCAT 
TATATGGGTA TTGGTATGGA CAAGCGAATG TTACATGCCA TGAAAGTGGG CAACTTGGCA 
AGCAATATAG CCTATAAATC TGATTACAAA CATGACGGTG TTGACTACAA TTACCCAGCT 
ACTCTCACTC CTTCATATCA GACAACAAGG AAGTTGGTCC CTTTGAAAGA TGTAAACTAC 
AGGCAAAGCA TAGACAAGAT GAAGTATAGT TCTGTTGCCA GCACTCCAGA AATTGCTCAA 
GCCAAAATAA ATGCTCAGCA GCTCAGTGAT TTGAACTACC GAGCCCAGTA TGAGAAGACA 
AAAACAAATT ACACTCTACC TCAGGACATA CCTCAGTTAG TCAAGGCAAA AGCCAATGCA 
GAATTATATA GTGAGGTTAA GTACAAAGAA GGCTGGGAGA AGAGCAAGGG CCAGGGATTT 
GAAATGAAGC TTGATTCTCT GCCTTTACTT GCTGCCAAAG CTTCAAGGGA TCTTGCCAGT 
GATATTAAGT ATAAAGAAGA ATATGAAAAA ACAAAAGGGA AAGCAATTGA AACAAAAGAT 
TCAAGACTTT TGCACTCTCT GCAGGTGGCC AAGATGAGTA GTGAGATTGC CTACAAAAAA 
GATTTTGAGG AGAGTAAGAC CCATTTCCAT CTGCCTATGG ACATGGTAAA CCTGAGGCAT 
GCTAAGAAGG CCCAAGCACT AGCTAGCGAC TTGGATTATA GAAAGAGACT CCATGAATAC 
ACAGTGGTTC CGGAAGATTT GAAGACAAAA TGGGCCAAGA AGGCCTATGG GCTCCAGAGT 
GATCTTCAAT ACAGGGAAGA CCTGATGTGG ATGAAAGGAG TTGGATGTAT CACAGAAGGA 
AGTCTTAACA TACAGCAAGC CAAAAAGGCA GGAGATTTGG TCAGTGAGAA AAAATACCGA 
CAGAAGGTTG ATGCTCTGAA ATTCACCAGT GTGGCTGACA GCTCTCACAT CAAACATGCC 
AAGAAAAGCC AGGAACTGCA AAGTGATAAC CGATACAGAG CAGATTTGAA CTGGATGAAA 
GGAGCTGGAT GGATAGCCAC TGGGTCATTA AATGTGGAGC AGGCTAAGAA GGCAGGAGAA 
CTCATTAGTG AGAAGAAGTA CCGTCAGCAT CCATATGCTT TGAAGTTTAC CAGTATTAAA 
GACACTCCTG AGATGATCCA GGCTAGAATT AGTTATAACC AGGCTGTGGA TCGGTTGTAC 
AAGGAGCATG GAGAAAGCAT AAAGCATCAG TACACACTAA CAGCAGATCT TCCTGAAATC 
CTTCGGGCTA AATTAAACAC CATGAATATC AGTGAGATTC GCTATAAGGA GTCCTGGCAA 
AAGATGAAGG ATGGTGGATA TCAGCTGAGA CTCGATGCCA TTCCCTTCCA GGCAGCTAAG 
GCTTCGGGTG AAATCATAAG CGATCACAAG TATAAGGAGG CATTTGAGAA AATGAAAGGG 
CAGATGATTG GTTCATGTGG CCTGGATGGT GATATTCGTA TTGCTCATTC AGTCCATGCG 
TCATCACTAC AGAGTGATGT GAAATATAAG CAAGGTTTTG AGGATACAAA GACTCACTTC 
CATCTGCCAC TCGATATGAT AAACTTGGTG CATGCCAGGA AAGCCCAGTC TTTGGTCAGT 
GAACAAGAAT ACAGACAGCT GCTACACCAG TACACTTCTT TGACTGATGA TCTGAGATTG 
CAGTGTGCCA AGAATGCATA TAAACTACAG AGTGAGAATT TATATCGGTC TGACATGAAC 
TTTATGCGAG GAGTCGGATG TATTACTCCA GGGGCCCTAG AGATTGAAGG AAAGAAGAAA 
GCCTCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCAGC CACATTCCTT CAAGTACACA 
TCGGTGACAG ACTCTCCTGG TCTCCTTCAT GCAAAATTCA GCAATATGAT TGCTAATGAG 
CGCCTTTATA AAGCAGCGGG AGAGGACATT CAGCATCACT ATACACCAAC ACTGGGTCTG 
CCTGAGTTCA CACAAGCAAG AATAAACGCA GCCAACCTCA GTGATGTGAA ATACAGGGAA 
TCTTGGCATA ATCTATGTGC TCAAGGCTAC AAGCTGACAA TGGAAGCACT CCCTTTCCAG 
ACTGCGAGGG CCTCCAGAGA AATTGCCAGT GATTACCAGT ATAAACACAA TTTTGTGATG 
GAGAGAGGAA AGCACATTGG GGCCCGAAGT GTTCTGGATG ATACCAGGTT GCTGCATTGC 
TTGCATGCTG CCAAATTACA GAGTGAACAG GAGTATAAGA AAGGATCCCA AGGAGTGTGG 
TCTCAGTACT ACCTGCCCAT GGACATGGTG AATCTTGTCC ATGCAAGGAA AGCCCAGGCC 
TTAGCAAGTG ATCAAGAATA TAGAAAGAGA CTCCATGAAT TTACAGCGCT CCCAGAAGAC 
TTGAAGATGA AATGGGCCAA AAGAGCCCAC ATGCTACAGA GTGAGCATCG CTATAAATCA 
GACCTGAACT TCATGAAAGG AGTTGGTTGG ATGGCTCTGA GATCTCCGCA GATAGAAAGT 
GTAAAGAAGG CTGGCGAGCT CATCAGTGAG ACCAAGTATC GTCAGAAGCC AGAAAGTTTG 
AAGTTCACCG CGGTGGTTGA CTCTCCAGAT TTGATTCATG CTAAAAATAG CTACCTCCAG 
TGCAATGACA GGCTGTACAA GGCTGGAGAC TCTGAAGCCA GGCACAGGTA TACCCTGCCT 
CCTGATCATC CAGATTTTAT CCGAGCTCGA CAGAATGCAC TTAATATCAG TGATAAAGTG 
TATAAAACAT CTTGGGAACA GACAAGAGCA TCTGGCTATG ATTTTAGGAT TGACGCCATT 
CCATTCCAGA CAGCAAAGGC TTCAAGAGAG ATTGCTAGTG ATTACAAATA CAGAGAAGCC 
TTCCTTCGAG ACAGAGGCCA GAGGATTGGA TTCTTCAGTG CAAATGATGA TGCCCACACC 
AGACATGTCC TCAGAGTAGG GAAACTCCAG AGCGACAACC TGTACAGGAG CGGATACGCC 
CAAAACAGAG GACATTTTCA GAGTCACCTT AACCAGCCTG GGTTCCTCCA TGCTAAGAGA 
AGCCAGCAGC TTGCCAGCAA TGTTAACTAC AAGCAGCCCT TGCACCAATA CACTTGTGAT 
CCTGAGCAGC TAAATGTGAA ACATGCAAAG CAGGCCTATA AGCTGCAAAG CGATGTAAAA 
TACAAGTCTG ACCTGAACTG GCTCAGAGGC ATTGGCTGGA CTCCTCCAGG TTCTTACAAA 
GTGGAAAGGG CAAGAAGAGC TGCTGAGCTG GCGTACCTTC GGGAAATGGG GTTGCAGGCT 
GCTTCTGCTC AGTATGGACC TGAAGCTGAC CAAGTGGGGC CAGAAGGATC ACAGCAGGTC 
ACAGTTAATC CTGATGCTTC GGAAATTCTG CAAGTCAAGA GAAGGAAAAT GCAGCTGTAC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144448.1
CDS	193..4878
Translation	MNVQPCARCGYGVYPAEKINCIDQTWHKACFHCEVCKMMLTVNNFVSHEKKPYCQVHNPKNNAFTSIFETPINLNAKKLSEVVSEVKYREESEQFKSAFQWDVRSRDIEAAYKAQHLSSQNAYYAAYEGGTSWYSGNMPDPQMVTVTQAQKNLNDLQYTEEYELRQGKGSFPAMITPGYQVAKRATQLSSNVEYRRGHEERVSKFTSVVDTPDILHAKAGGQLASDLKYTEDFEEQRGKGSFPAMITPAYQIAKRANELASDVKYHQTYEKEIKGKASHTAGTDVTFTRENVDQYGQDYMNEYEERRGKGSFPAMITPAYQNAKKANELASDIKYKKDLSKMKGAAHFHSLTAEDNLVLKQAQSANKLVSEVEYKKDLGNNRGYSVNYCDTPQFKNVSKISKYTSDIKYKETYQNQMKGHYMGIGMDKRMLHAMKVGNLASNIAYKSDYKHDGVDYNYPATLTPSYQTTRKLVPLKDVNYRQSIDKMKYSSVASTPEIAQAKINAQQLSDLNYRAQYEKTKTNYTLPQDIPQLVKAKANAELYSEVKYKEGWEKSKGQGFEMKLDSLPLLAAKASRDLASDIKYKEEYEKTKGKAIETKDSRLLHSLQVAKMSSEIAYKKDFEESKTHFHLPMDMVNLRHAKKAQALASDLDYRKRLHEYTVVPEDLKTKWAKKAYGLQSDLQYREDLMWMKGVGCITEGSLNIQQAKKAGDLVSEKKYRQKVDALKFTSVADSSHIKHAKKSQELQSDNRYRADLNWMKGAGWIATGSLNVEQAKKAGELISEKKYRQHPYALKFTSIKDTPEMIQARISYNQAVDRLYKEHGESIKHQYTLTADLPEILRAKLNTMNISEIRYKESWQKMKDGGYQLRLDAIPFQAAKASGEIISDHKYKEAFEKMKGQMIGSCGLDGDIRIAHSVHASSLQSDVKYKQGFEDTKTHFHLPLDMINLVHARKAQSLVSEQEYRQLLHQYTSLTDDLRLQCAKNAYKLQSENLYRSDMNFMRGVGCITPGALEIEGKKKASELISESKYRQQPHSFKYTSVTDSPGLLHAKFSNMIANERLYKAAGEDIQHHYTPTLGLPEFTQARINAANLSDVKYRESWHNLCAQGYKLTMEALPFQTARASREIASDYQYKHNFVMERGKHIGARSVLDDTRLLHCLHAAKLQSEQEYKKGSQGVWSQYYLPMDMVNLVHARKAQALASDQEYRKRLHEFTALPEDLKMKWAKRAHMLQSEHRYKSDLNFMKGVGWMALRSPQIESVKKAGELISETKYRQKPESLKFTAVVDSPDLIHAKNSYLQCNDRLYKAGDSEARHRYTLPPDHPDFIRARQNALNISDKVYKTSWEQTRASGYDFRIDAIPFQTAKASREIASDYKYREAFLRDRGQRIGFFSANDDAHTRHVLRVGKLQSDNLYRSGYAQNRGHFQSHLNQPGFLHAKRSQQLASNVNYKQPLHQYTCDPEQLNVKHAKQAYKLQSDVKYKSDLNWLRGIGWTPPGSYKVERARRAAELAYLREMGLQAASAQYGPEADQVGPEGSQQVTVNPDASEILQVKRRKMQLYK

Target ORF information:

RefSeq Version	XM_015288962.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288962.1

ORF Insert Sequence:

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA GTGAGGTAAA GTATCGAGAG GAGAGTGAGC AATTCAAATC TGCCTTCCAG 
TGGGATGTGA GGTCCAGAGA CATTGAAGCT GCTTATAAAG CCCAACATCT GTCCAGCCAG 
AATGCTTACT ATGCTGCCTA TGAGGGGGGA ACGTCATGGT ACTCAGGAAA CATGCCAGAT 
CCTCAGATGG TCACAGTAAC TCAGGCACAG AAGAATCTGA ATGATCTCCA ATATACAGAA 
GAATATGAGC TGCGTCAAGG CAAAGGCAGC TTTCCTGCTA TGATCACTCC AGGTTATCAG 
GTGGCTAAGA GAGCCACTCA ACTAAGCAGT AATGTGGAAT ATAGGCGGGG ACATGAAGAG 
AGAGTTTCAA AGTTCACCTC TGTTGTAGAC ACTCCAGATA TACTTCACGC AAAGGCTGGA 
GGGCAGCTTG CAAGTGATTT GAAATACACT GAAGACTTTG AAGAACAGAG AGGGAAAGGC 
AGCTTTCCTG CTATGATCAC TCCAGCTTAT CAAATTGCCA AGAGAGCAAA TGAATTAGCC 
AGTGATGTAA AGTATCATCA AACGTACGAA AAAGAAATCA AGGGAAAAGC CAGCCATACA 
GCTGGAACAG ATGTTACTTT TACAAGAGAA AACGTAGATC AATATGGCCA GGATTATATG 
AATGAGTATG AAGAACGCAG AGGGAAAGGA AGCTTTCCTG CTATGATCAC TCCAGCTTAT 
CAAAATGCCA AGAAGGCAAA TGAATTGGCA AGTGATATAA AATACAAGAA AGATCTTTCC 
AAGATGAAAG GTGCAGCTCA CTTTCACTCT CTAACAGCTG AAGACAACCT GGTCCTTAAA 
CAAGCCCAGA GTGCTAACAA GCTTGTTAGT GAGGTGGAAT ACAAGAAGGA TCTTGGAAAC 
AACAGAGGCT ACAGCGTTAA CTACTGTGAT ACTCCTCAAT TCAAGAATGT GAGCAAGATC 
TCAAAATACA CCAGTGATAT TAAGTACAAA GAAACGTACC AGAACCAAAT GAAAGGCCAT 
TATATGGGTA TTGGTATGGA CAAGCGAATG TTACATGCCA TGAAAGTGGG CAACTTGGCA 
AGCAATATAG CCTATAAATC TGATTACAAA CATGACGGTG TTGACTACAA TTACCCAGCT 
ACTCTCACTC CTTCATATCA GACAACAAGG AAGTTGGTCC CTTTGAAAGA TGTAAACTAC 
AGGCAAAGCA TAGACAAGAT GAAGTATAGT TCTGTTGCCA GCACTCCAGA AATTGCTCAA 
GCCAAAATAA ATGCTCAGCA GCTCAGTGAT TTGAACTACC GAGCCCAGTA TGAGAAGACA 
AAAACAAATT ACACTCTACC TCAGGACATA CCTCAGTTAG TCAAGGCAAA AGCCAATGCA 
GAATTATATA GTGAGGTTAA GTACAAAGAA GGCTGGGAGA AGAGCAAGGG CCAGGGATTT 
GAAATGAAGC TTGATTCTCT GCCTTTACTT GCTGCCAAAG CTTCAAGGGA TCTTGCCAGT 
GATATTAAGT ATAAAGAAGA ATATGAAAAA ACAAAAGGGA AAGCAATTGA AACAAAAGAT 
TCAAGACTTT TGCACTCTCT GCAGGTGGCC AAGATGAGTA GTGAGATTGC CTACAAAAAA 
GATTTTGAGG AGAGTAAGAC CCATTTCCAT CTGCCTATGG ACATGGTAAA CCTGAGGCAT 
GCTAAGAAGG CCCAAGCACT AGCTAGCGAC TTGGATTATA GAAAGAGACT CCATGAATAC 
ACAGTGGTTC CGGAAGATTT GAAGACAAAA TGGGCCAAGA AGGCCTATGG GCTCCAGAGT 
GATCTTCAAT ACAGGGAAGA CCTGATGTGG ATGAAAGGAG TTGGATGTAT CACAGAAGGA 
AGTCTTAACA TACAGCAAGC CAAAAAGGCA GGAGATTTGG TCAGTGAGAA AAAATACCGA 
CAGAAGGTTG ATGCTCTGAA ATTCACCAGT GTGGCTGACA GCTCTCACAT CAAACATGCC 
AAGAAAAGCC AGGAACTGCA AAGTGATAAC CGATACAGAG CAGATTTGAA CTGGATGAAA 
GGAGCTGGAT GGATAGCCAC TGGGTCATTA AATGTGGAGC AGGCTAAGAA GGCAGGAGAA 
CTCATTAGTG AGAAGAAGTA CCGTCAGCAT CCATATGCTT TGAAGTTTAC CAGTATTAAA 
GACACTCCTG AGATGATCCA GGCTAGAATT AGTTATAACC AGGCTGTGGA TCGGTTGTAC 
AAGGAGCATG GAGAAAGCAT AAAGCATCAG TACACACTAA CAGCAGATCT TCCTGAAATC 
CTTCGGGCTA AATTAAACAC CATGAATATC AGTGAGATTC GCTATAAGGA GTCCTGGCAA 
AAGATGAAGG ATGGTGGATA TCAGCTGAGA CTCGATGCCA TTCCCTTCCA GGCAGCTAAG 
GCTTCGGGTG AAATCATAAG CGATCACAAG TATAAGGAGG CATTTGAGAA AATGAAAGGG 
CAGATGATTG GTTCATGTGG CCTGGATGGT GATATTCGTA TTGCTCATTC AGTCCATGCG 
TCATCACTAC AGAGTGATGT GAAATATAAG CAAGGTTTTG AGGATACAAA GACTCACTTC 
CATCTGCCAC TCGATATGAT AAACTTGGTG CATGCCAGGA AAGCCCAGTC TTTGGTCAGT 
GAACAAGAAT ACAGACAGCT GCTACACCAG TACACTTCTT TGACTGATGA TCTGAGATTG 
CAGTGTGCCA AGAATGCATA TAAACTACAG AGTGAGAATT TATATCGGTC TGACATGAAC 
TTTATGCGAG GAGTCGGATG TATTACTCCA GGGGCCCTAG AGATTGAAGG AAAGAAGAAA 
GCCTCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCAGC CACATTCCTT CAAGTACACA 
TCGGTGACAG ACTCTCCTGG TCTCCTTCAT GCAAAATTCA GCAATATGAT TGCTAATGAG 
CGCCTTTATA AAGCAGCGGG AGAGGACATT CAGCATCACT ATACACCAAC ACTGGGTCTG 
CCTGAGTTCA CACAAGCAAG AATAAACGCA GCCAACCTCA GTGATGTGAA ATACAGGGAA 
TCTTGGCATA ATCTATGTGC TCAAGGCTAC AAGCTGACAA TGGAAGCACT CCCTTTCCAG 
ACTGCGAGGG CCTCCAGAGA AATTGCCAGT GATTACCAGT ATAAACACAA TTTTGTGATG 
GAGAGAGGAA AGCACATTGG GGCCCGAAGT GTTCTGGATG ATACCAGGTT GCTGCATTGC 
TTGCATGCTG CCAAATTACA GAGTGAACAG GAGTATAAGA AAGGATCCCA AGGAGTGTGG 
TCTCAGTACT ACCTGCCCAT GGACATGGTG AATCTTGTCC ATGCAAGGAA AGCCCAGGCC 
TTAGCAAGTG ATCAAGAATA TAGAAAGAGA CTCCATGAAT TTACAGCGCT CCCAGAAGAC 
TTGAAGATGA AATGGGCCAA AAGAGCCCAC ATGCTACAGA GTGAGCATCG CTATAAATCA 
GACCTGAACT TCATGAAAGG AGTTGGTTGG ATGGCTCTGA GATCTCCGCA GATAGAAAGT 
GTAAAGAAGG CTGGCGAGCT CATCAGTGAG ACCAAGTATC GTCAGAAGCC AGAAAGTTTG 
AAGTTCACCG CGGTGGTTGA CTCTCCAGAT TTGATTCATG CTAAAAATAG CTACCTCCAG 
TGCAATGACA GGCTGTACAA GGCTGGAGAC TCTGAAGCCA GGCACAGGTA TACCCTGCCT 
CCTGATCATC CAGATTTTAT CCGAGCTCGA CAGAATGCAC TTAATATCAG TGATAAAGTG 
TATAAAACAT CTTGGGAACA GACAAGAGCA TCTGGCTATG ATTTTAGGAT TGACGCCATT 
CCATTCCAGA CAGCAAAGGC TTCAAGAGAG ATTGCTAGTG ATTACAAATA CAGAGAAGCC 
TTCCTTCGAG ACAGAGGCCA GAGGATTGGA TTCTTCAGTG CAAATGATGA TGCCCACACC 
AGACATGTCC TCAGAGTAGG GAAACTCCAG AGCGACAACC TGTACAGGAG CGGATACGCC 
CAAAACAGAG GACATTTTCA GAGTCACCTT AACCAGCCTG GGTTCCTCCA TGCTAAGAGA 
AGCCAGCAGC TTGCCAGCAA TGTTAACTAC AAGCAGCCCT TGCACCAATA CACTTGTGAT 
CCTGAGCAGC TAAATGTGAA ACATGCAAAG CAGGCCTATA AGCTGCAAAG CGATGTAAAA 
TACAAGTCTG ACCTGAACTG GCTCAGAGGC ATTGGCTGGA CTCCTCCAGG TTCTTACAAA 
GTGGAAAGGG CAAGAAGAGC TGCTGAGCTG GCGTACCTTC GGGAAATGGG GTTGCAGGCT 
GCTTCTGCTC AGTATGGACC TGAAGCTGAC CAAGTGGGGC CAGAAGGATC ACAGCAGGTC 
ACAGTTAATC CTGATGCTTC GGAAATTCTG CAAGTCAAGA GAAGGAAAAT GCAGCTGTAC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_015288962.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144448.1 Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X4, mRNA.

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CloneID	OGa38032
Clone ID Related Accession (Same CDS sequence)	XM_015288962.1 , XM_015288962.2
Accession Version	XM_015288962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	nebulin-related-anchoring protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015288962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA GTGAGGTAAA GTATCGAGAG GAGAGTGAGC AATTCAAATC TGCCTTCCAG 
TGGGATGTGA GGTCCAGAGA CATTGAAGCT GCTTATAAAG CCCAACATCT GTCCAGCCAG 
AATGCTTACT ATGCTGCCTA TGAGGGGGGA ACGTCATGGT ACTCAGGAAA CATGCCAGAT 
CCTCAGATGG TCACAGTAAC TCAGGCACAG AAGAATCTGA ATGATCTCCA ATATACAGAA 
GAATATGAGC TGCGTCAAGG CAAAGGCAGC TTTCCTGCTA TGATCACTCC AGGTTATCAG 
GTGGCTAAGA GAGCCACTCA ACTAAGCAGT AATGTGGAAT ATAGGCGGGG ACATGAAGAG 
AGAGTTTCAA AGTTCACCTC TGTTGTAGAC ACTCCAGATA TACTTCACGC AAAGGCTGGA 
GGGCAGCTTG CAAGTGATTT GAAATACACT GAAGACTTTG AAGAACAGAG AGGGAAAGGC 
AGCTTTCCTG CTATGATCAC TCCAGCTTAT CAAATTGCCA AGAGAGCAAA TGAATTAGCC 
AGTGATGTAA AGTATCATCA AACGTACGAA AAAGAAATCA AGGGAAAAGC CAGCCATACA 
GCTGGAACAG ATGTTACTTT TACAAGAGAA AACGTAGATC AATATGGCCA GGATTATATG 
AATGAGTATG AAGAACGCAG AGGGAAAGGA AGCTTTCCTG CTATGATCAC TCCAGCTTAT 
CAAAATGCCA AGAAGGCAAA TGAATTGGCA AGTGATATAA AATACAAGAA AGATCTTTCC 
AAGATGAAAG GTGCAGCTCA CTTTCACTCT CTAACAGCTG AAGACAACCT GGTCCTTAAA 
CAAGCCCAGA GTGCTAACAA GCTTGTTAGT GAGGTGGAAT ACAAGAAGGA TCTTGGAAAC 
AACAGAGGCT ACAGCGTTAA CTACTGTGAT ACTCCTCAAT TCAAGAATGT GAGCAAGATC 
TCAAAATACA CCAGTGATAT TAAGTACAAA GAAACGTACC AGAACCAAAT GAAAGGCCAT 
TATATGGGTA TTGGTATGGA CAAGCGAATG TTACATGCCA TGAAAGTGGG CAACTTGGCA 
AGCAATATAG CCTATAAATC TGATTACAAA CATGACGGTG TTGACTACAA TTACCCAGCT 
ACTCTCACTC CTTCATATCA GACAACAAGG AAGTTGGTCC CTTTGAAAGA TGTAAACTAC 
AGGCAAAGCA TAGACAAGAT GAAGTATAGT TCTGTTGCCA GCACTCCAGA AATTGCTCAA 
GCCAAAATAA ATGCTCAGCA GCTCAGTGAT TTGAACTACC GAGCCCAGTA TGAGAAGACA 
AAAACAAATT ACACTCTACC TCAGGACATA CCTCAGTTAG TCAAGGCAAA AGCCAATGCA 
GAATTATATA GTGAGGTTAA GTACAAAGAA GGCTGGGAGA AGAGCAAGGG CCAGGGATTT 
GAAATGAAGC TTGATTCTCT GCCTTTACTT GCTGCCAAAG CTTCAAGGGA TCTTGCCAGT 
GATATTAAGT ATAAAGAAGA ATATGAAAAA ACAAAAGGGA AAGCAATTGA AACAAAAGAT 
TCAAGACTTT TGCACTCTCT GCAGGTGGCC AAGATGAGTA GTGAGATTGC CTACAAAAAA 
GATTTTGAGG AGAGTAAGAC CCATTTCCAT CTGCCTATGG ACATGGTAAA CCTGAGGCAT 
GCTAAGAAGG CCCAAGCACT AGCTAGCGAC TTGGATTATA GAAAGAGACT CCATGAATAC 
ACAGTGGTTC CGGAAGATTT GAAGACAAAA TGGGCCAAGA AGGCCTATGG GCTCCAGAGT 
GATCTTCAAT ACAGGGAAGA CCTGATGTGG ATGAAAGGAG TTGGATGTAT CACAGAAGGA 
AGTCTTAACA TACAGCAAGC CAAAAAGGCA GGAGATTTGG TCAGTGAGAA AAAATACCGA 
CAGAAGGTTG ATGCTCTGAA ATTCACCAGT GTGGCTGACA GCTCTCACAT CAAACATGCC 
AAGAAAAGCC AGGAACTGCA AAGTGATAAC CGATACAGAG CAGATTTGAA CTGGATGAAA 
GGAGCTGGAT GGATAGCCAC TGGGTCATTA AATGTGGAGC AGGCTAAGAA GGCAGGAGAA 
CTCATTAGTG AGAAGAAGTA CCGTCAGCAT CCATATGCTT TGAAGTTTAC CAGTATTAAA 
GACACTCCTG AGATGATCCA GGCTAGAATT AGTTATAACC AGGCTGTGGA TCGGTTGTAC 
AAGGAGCATG GAGAAAGCAT AAAGCATCAG TACACACTAA CAGCAGATCT TCCTGAAATC 
CTTCGGGCTA AATTAAACAC CATGAATATC AGTGAGATTC GCTATAAGGA GTCCTGGCAA 
AAGATGAAGG ATGGTGGATA TCAGCTGAGA CTCGATGCCA TTCCCTTCCA GGCAGCTAAG 
GCTTCGGGTG AAATCATAAG CGATCACAAG TATAAGGAGG CATTTGAGAA AATGAAAGGG 
CAGATGATTG GTTCATGTGG CCTGGATGGT GATATTCGTA TTGCTCATTC AGTCCATGCG 
TCATCACTAC AGAGTGATGT GAAATATAAG CAAGGTTTTG AGGATACAAA GACTCACTTC 
CATCTGCCAC TCGATATGAT AAACTTGGTG CATGCCAGGA AAGCCCAGTC TTTGGTCAGT 
GAACAAGAAT ACAGACAGCT GCTACACCAG TACACTTCTT TGACTGATGA TCTGAGATTG 
CAGTGTGCCA AGAATGCATA TAAACTACAG AGTGAGAATT TATATCGGTC TGACATGAAC 
TTTATGCGAG GAGTCGGATG TATTACTCCA GGGGCCCTAG AGATTGAAGG AAAGAAGAAA 
GCCTCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCAGC CACATTCCTT CAAGTACACA 
TCGGTGACAG ACTCTCCTGG TCTCCTTCAT GCAAAATTCA GCAATATGAT TGCTAATGAG 
CGCCTTTATA AAGCAGCGGG AGAGGACATT CAGCATCACT ATACACCAAC ACTGGGTCTG 
CCTGAGTTCA CACAAGCAAG AATAAACGCA GCCAACCTCA GTGATGTGAA ATACAGGGAA 
TCTTGGCATA ATCTATGTGC TCAAGGCTAC AAGCTGACAA TGGAAGCACT CCCTTTCCAG 
ACTGCGAGGG CCTCCAGAGA AATTGCCAGT GATTACCAGT ATAAACACAA TTTTGTGATG 
GAGAGAGGAA AGCACATTGG GGCCCGAAGT GTTCTGGATG ATACCAGGTT GCTGCATTGC 
TTGCATGCTG CCAAATTACA GAGTGAACAG GAGTATAAGA AAGGATCCCA AGGAGTGTGG 
TCTCAGTACT ACCTGCCCAT GGACATGGTG AATCTTGTCC ATGCAAGGAA AGCCCAGGCC 
TTAGCAAGTG ATCAAGAATA TAGAAAGAGA CTCCATGAAT TTACAGCGCT CCCAGAAGAC 
TTGAAGATGA AATGGGCCAA AAGAGCCCAC ATGCTACAGA GTGAGCATCG CTATAAATCA 
GACCTGAACT TCATGAAAGG AGTTGGTTGG ATGGCTCTGA GATCTCCGCA GATAGAAAGT 
GTAAAGAAGG CTGGCGAGCT CATCAGTGAG ACCAAGTATC GTCAGAAGCC AGAAAGTTTG 
AAGTTCACCG CGGTGGTTGA CTCTCCAGAT TTGATTCATG CTAAAAATAG CTACCTCCAG 
TGCAATGACA GGCTGTACAA GGCTGGAGAC TCTGAAGCCA GGCACAGGTA TACCCTGCCT 
CCTGATCATC CAGATTTTAT CCGAGCTCGA CAGAATGCAC TTAATATCAG TGATAAAGTG 
TATAAAACAT CTTGGGAACA GACAAGAGCA TCTGGCTATG ATTTTAGGAT TGACGCCATT 
CCATTCCAGA CAGCAAAGGC TTCAAGAGAG ATTGCTAGTG ATTACAAATA CAGAGAAGCC 
TTCCTTCGAG ACAGAGGCCA GAGGATTGGA TTCTTCAGTG CAAATGATGA TGCCCACACC 
AGACATGTCC TCAGAGTAGG GAAACTCCAG AGCGACAACC TGTACAGGAG CGGATACGCC 
CAAAACAGAG GACATTTTCA GAGTCACCTT AACCAGCCTG GGTTCCTCCA TGCTAAGAGA 
AGCCAGCAGC TTGCCAGCAA TGTTAACTAC AAGCAGCCCT TGCACCAATA CACTTGTGAT 
CCTGAGCAGC TAAATGTGAA ACATGCAAAG CAGGCCTATA AGCTGCAAAG CGATGTAAAA 
TACAAGTCTG ACCTGAACTG GCTCAGAGGC ATTGGCTGGA CTCCTCCAGG TTCTTACAAA 
GTGGAAAGGG CAAGAAGAGC TGCTGAGCTG GCGTACCTTC GGGAAATGGG GTTGCAGGCT 
GCTTCTGCTC AGTATGGACC TGAAGCTGAC CAAGTGGGGC CAGAAGGATC ACAGCAGGTC 
ACAGTTAATC CTGATGCTTC GGAAATTCTG CAAGTCAAGA GAAGGAAAAT GCAGCTGTAC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144448.1
CDS	198..4883
Translation	MNVQPCARCGYGVYPAEKINCIDQTWHKACFHCEVCKMMLTVNNFVSHEKKPYCQVHNPKNNAFTSIFETPINLNAKKLSEVVSEVKYREESEQFKSAFQWDVRSRDIEAAYKAQHLSSQNAYYAAYEGGTSWYSGNMPDPQMVTVTQAQKNLNDLQYTEEYELRQGKGSFPAMITPGYQVAKRATQLSSNVEYRRGHEERVSKFTSVVDTPDILHAKAGGQLASDLKYTEDFEEQRGKGSFPAMITPAYQIAKRANELASDVKYHQTYEKEIKGKASHTAGTDVTFTRENVDQYGQDYMNEYEERRGKGSFPAMITPAYQNAKKANELASDIKYKKDLSKMKGAAHFHSLTAEDNLVLKQAQSANKLVSEVEYKKDLGNNRGYSVNYCDTPQFKNVSKISKYTSDIKYKETYQNQMKGHYMGIGMDKRMLHAMKVGNLASNIAYKSDYKHDGVDYNYPATLTPSYQTTRKLVPLKDVNYRQSIDKMKYSSVASTPEIAQAKINAQQLSDLNYRAQYEKTKTNYTLPQDIPQLVKAKANAELYSEVKYKEGWEKSKGQGFEMKLDSLPLLAAKASRDLASDIKYKEEYEKTKGKAIETKDSRLLHSLQVAKMSSEIAYKKDFEESKTHFHLPMDMVNLRHAKKAQALASDLDYRKRLHEYTVVPEDLKTKWAKKAYGLQSDLQYREDLMWMKGVGCITEGSLNIQQAKKAGDLVSEKKYRQKVDALKFTSVADSSHIKHAKKSQELQSDNRYRADLNWMKGAGWIATGSLNVEQAKKAGELISEKKYRQHPYALKFTSIKDTPEMIQARISYNQAVDRLYKEHGESIKHQYTLTADLPEILRAKLNTMNISEIRYKESWQKMKDGGYQLRLDAIPFQAAKASGEIISDHKYKEAFEKMKGQMIGSCGLDGDIRIAHSVHASSLQSDVKYKQGFEDTKTHFHLPLDMINLVHARKAQSLVSEQEYRQLLHQYTSLTDDLRLQCAKNAYKLQSENLYRSDMNFMRGVGCITPGALEIEGKKKASELISESKYRQQPHSFKYTSVTDSPGLLHAKFSNMIANERLYKAAGEDIQHHYTPTLGLPEFTQARINAANLSDVKYRESWHNLCAQGYKLTMEALPFQTARASREIASDYQYKHNFVMERGKHIGARSVLDDTRLLHCLHAAKLQSEQEYKKGSQGVWSQYYLPMDMVNLVHARKAQALASDQEYRKRLHEFTALPEDLKMKWAKRAHMLQSEHRYKSDLNFMKGVGWMALRSPQIESVKKAGELISETKYRQKPESLKFTAVVDSPDLIHAKNSYLQCNDRLYKAGDSEARHRYTLPPDHPDFIRARQNALNISDKVYKTSWEQTRASGYDFRIDAIPFQTAKASREIASDYKYREAFLRDRGQRIGFFSANDDAHTRHVLRVGKLQSDNLYRSGYAQNRGHFQSHLNQPGFLHAKRSQQLASNVNYKQPLHQYTCDPEQLNVKHAKQAYKLQSDVKYKSDLNWLRGIGWTPPGSYKVERARRAAELAYLREMGLQAASAQYGPEADQVGPEGSQQVTVNPDASEILQVKRRKMQLYK

Target ORF information:

RefSeq Version	XM_015288962.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nebulin-related anchoring protein (NRAP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015288962.2

ORF Insert Sequence:

ATGAATGTGC AGCCGTGTGC TAGATGTGGC TATGGGGTTT ACCCTGCTGA GAAGATTAAC 
TGTATAGATC AGACGTGGCA TAAAGCCTGT TTTCACTGTG AAGTGTGCAA GATGATGTTA 
ACAGTAAACA ACTTTGTTAG CCATGAGAAA AAGCCTTACT GTCAAGTGCA CAACCCAAAA 
AACAATGCCT TCACAAGCAT ATTTGAGACA CCAATAAATT TGAATGCCAA AAAACTATCG 
GAAGTGGTAA GTGAGGTAAA GTATCGAGAG GAGAGTGAGC AATTCAAATC TGCCTTCCAG 
TGGGATGTGA GGTCCAGAGA CATTGAAGCT GCTTATAAAG CCCAACATCT GTCCAGCCAG 
AATGCTTACT ATGCTGCCTA TGAGGGGGGA ACGTCATGGT ACTCAGGAAA CATGCCAGAT 
CCTCAGATGG TCACAGTAAC TCAGGCACAG AAGAATCTGA ATGATCTCCA ATATACAGAA 
GAATATGAGC TGCGTCAAGG CAAAGGCAGC TTTCCTGCTA TGATCACTCC AGGTTATCAG 
GTGGCTAAGA GAGCCACTCA ACTAAGCAGT AATGTGGAAT ATAGGCGGGG ACATGAAGAG 
AGAGTTTCAA AGTTCACCTC TGTTGTAGAC ACTCCAGATA TACTTCACGC AAAGGCTGGA 
GGGCAGCTTG CAAGTGATTT GAAATACACT GAAGACTTTG AAGAACAGAG AGGGAAAGGC 
AGCTTTCCTG CTATGATCAC TCCAGCTTAT CAAATTGCCA AGAGAGCAAA TGAATTAGCC 
AGTGATGTAA AGTATCATCA AACGTACGAA AAAGAAATCA AGGGAAAAGC CAGCCATACA 
GCTGGAACAG ATGTTACTTT TACAAGAGAA AACGTAGATC AATATGGCCA GGATTATATG 
AATGAGTATG AAGAACGCAG AGGGAAAGGA AGCTTTCCTG CTATGATCAC TCCAGCTTAT 
CAAAATGCCA AGAAGGCAAA TGAATTGGCA AGTGATATAA AATACAAGAA AGATCTTTCC 
AAGATGAAAG GTGCAGCTCA CTTTCACTCT CTAACAGCTG AAGACAACCT GGTCCTTAAA 
CAAGCCCAGA GTGCTAACAA GCTTGTTAGT GAGGTGGAAT ACAAGAAGGA TCTTGGAAAC 
AACAGAGGCT ACAGCGTTAA CTACTGTGAT ACTCCTCAAT TCAAGAATGT GAGCAAGATC 
TCAAAATACA CCAGTGATAT TAAGTACAAA GAAACGTACC AGAACCAAAT GAAAGGCCAT 
TATATGGGTA TTGGTATGGA CAAGCGAATG TTACATGCCA TGAAAGTGGG CAACTTGGCA 
AGCAATATAG CCTATAAATC TGATTACAAA CATGACGGTG TTGACTACAA TTACCCAGCT 
ACTCTCACTC CTTCATATCA GACAACAAGG AAGTTGGTCC CTTTGAAAGA TGTAAACTAC 
AGGCAAAGCA TAGACAAGAT GAAGTATAGT TCTGTTGCCA GCACTCCAGA AATTGCTCAA 
GCCAAAATAA ATGCTCAGCA GCTCAGTGAT TTGAACTACC GAGCCCAGTA TGAGAAGACA 
AAAACAAATT ACACTCTACC TCAGGACATA CCTCAGTTAG TCAAGGCAAA AGCCAATGCA 
GAATTATATA GTGAGGTTAA GTACAAAGAA GGCTGGGAGA AGAGCAAGGG CCAGGGATTT 
GAAATGAAGC TTGATTCTCT GCCTTTACTT GCTGCCAAAG CTTCAAGGGA TCTTGCCAGT 
GATATTAAGT ATAAAGAAGA ATATGAAAAA ACAAAAGGGA AAGCAATTGA AACAAAAGAT 
TCAAGACTTT TGCACTCTCT GCAGGTGGCC AAGATGAGTA GTGAGATTGC CTACAAAAAA 
GATTTTGAGG AGAGTAAGAC CCATTTCCAT CTGCCTATGG ACATGGTAAA CCTGAGGCAT 
GCTAAGAAGG CCCAAGCACT AGCTAGCGAC TTGGATTATA GAAAGAGACT CCATGAATAC 
ACAGTGGTTC CGGAAGATTT GAAGACAAAA TGGGCCAAGA AGGCCTATGG GCTCCAGAGT 
GATCTTCAAT ACAGGGAAGA CCTGATGTGG ATGAAAGGAG TTGGATGTAT CACAGAAGGA 
AGTCTTAACA TACAGCAAGC CAAAAAGGCA GGAGATTTGG TCAGTGAGAA AAAATACCGA 
CAGAAGGTTG ATGCTCTGAA ATTCACCAGT GTGGCTGACA GCTCTCACAT CAAACATGCC 
AAGAAAAGCC AGGAACTGCA AAGTGATAAC CGATACAGAG CAGATTTGAA CTGGATGAAA 
GGAGCTGGAT GGATAGCCAC TGGGTCATTA AATGTGGAGC AGGCTAAGAA GGCAGGAGAA 
CTCATTAGTG AGAAGAAGTA CCGTCAGCAT CCATATGCTT TGAAGTTTAC CAGTATTAAA 
GACACTCCTG AGATGATCCA GGCTAGAATT AGTTATAACC AGGCTGTGGA TCGGTTGTAC 
AAGGAGCATG GAGAAAGCAT AAAGCATCAG TACACACTAA CAGCAGATCT TCCTGAAATC 
CTTCGGGCTA AATTAAACAC CATGAATATC AGTGAGATTC GCTATAAGGA GTCCTGGCAA 
AAGATGAAGG ATGGTGGATA TCAGCTGAGA CTCGATGCCA TTCCCTTCCA GGCAGCTAAG 
GCTTCGGGTG AAATCATAAG CGATCACAAG TATAAGGAGG CATTTGAGAA AATGAAAGGG 
CAGATGATTG GTTCATGTGG CCTGGATGGT GATATTCGTA TTGCTCATTC AGTCCATGCG 
TCATCACTAC AGAGTGATGT GAAATATAAG CAAGGTTTTG AGGATACAAA GACTCACTTC 
CATCTGCCAC TCGATATGAT AAACTTGGTG CATGCCAGGA AAGCCCAGTC TTTGGTCAGT 
GAACAAGAAT ACAGACAGCT GCTACACCAG TACACTTCTT TGACTGATGA TCTGAGATTG 
CAGTGTGCCA AGAATGCATA TAAACTACAG AGTGAGAATT TATATCGGTC TGACATGAAC 
TTTATGCGAG GAGTCGGATG TATTACTCCA GGGGCCCTAG AGATTGAAGG AAAGAAGAAA 
GCCTCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCAGC CACATTCCTT CAAGTACACA 
TCGGTGACAG ACTCTCCTGG TCTCCTTCAT GCAAAATTCA GCAATATGAT TGCTAATGAG 
CGCCTTTATA AAGCAGCGGG AGAGGACATT CAGCATCACT ATACACCAAC ACTGGGTCTG 
CCTGAGTTCA CACAAGCAAG AATAAACGCA GCCAACCTCA GTGATGTGAA ATACAGGGAA 
TCTTGGCATA ATCTATGTGC TCAAGGCTAC AAGCTGACAA TGGAAGCACT CCCTTTCCAG 
ACTGCGAGGG CCTCCAGAGA AATTGCCAGT GATTACCAGT ATAAACACAA TTTTGTGATG 
GAGAGAGGAA AGCACATTGG GGCCCGAAGT GTTCTGGATG ATACCAGGTT GCTGCATTGC 
TTGCATGCTG CCAAATTACA GAGTGAACAG GAGTATAAGA AAGGATCCCA AGGAGTGTGG 
TCTCAGTACT ACCTGCCCAT GGACATGGTG AATCTTGTCC ATGCAAGGAA AGCCCAGGCC 
TTAGCAAGTG ATCAAGAATA TAGAAAGAGA CTCCATGAAT TTACAGCGCT CCCAGAAGAC 
TTGAAGATGA AATGGGCCAA AAGAGCCCAC ATGCTACAGA GTGAGCATCG CTATAAATCA 
GACCTGAACT TCATGAAAGG AGTTGGTTGG ATGGCTCTGA GATCTCCGCA GATAGAAAGT 
GTAAAGAAGG CTGGCGAGCT CATCAGTGAG ACCAAGTATC GTCAGAAGCC AGAAAGTTTG 
AAGTTCACCG CGGTGGTTGA CTCTCCAGAT TTGATTCATG CTAAAAATAG CTACCTCCAG 
TGCAATGACA GGCTGTACAA GGCTGGAGAC TCTGAAGCCA GGCACAGGTA TACCCTGCCT 
CCTGATCATC CAGATTTTAT CCGAGCTCGA CAGAATGCAC TTAATATCAG TGATAAAGTG 
TATAAAACAT CTTGGGAACA GACAAGAGCA TCTGGCTATG ATTTTAGGAT TGACGCCATT 
CCATTCCAGA CAGCAAAGGC TTCAAGAGAG ATTGCTAGTG ATTACAAATA CAGAGAAGCC 
TTCCTTCGAG ACAGAGGCCA GAGGATTGGA TTCTTCAGTG CAAATGATGA TGCCCACACC 
AGACATGTCC TCAGAGTAGG GAAACTCCAG AGCGACAACC TGTACAGGAG CGGATACGCC 
CAAAACAGAG GACATTTTCA GAGTCACCTT AACCAGCCTG GGTTCCTCCA TGCTAAGAGA 
AGCCAGCAGC TTGCCAGCAA TGTTAACTAC AAGCAGCCCT TGCACCAATA CACTTGTGAT 
CCTGAGCAGC TAAATGTGAA ACATGCAAAG CAGGCCTATA AGCTGCAAAG CGATGTAAAA 
TACAAGTCTG ACCTGAACTG GCTCAGAGGC ATTGGCTGGA CTCCTCCAGG TTCTTACAAA 
GTGGAAAGGG CAAGAAGAGC TGCTGAGCTG GCGTACCTTC GGGAAATGGG GTTGCAGGCT 
GCTTCTGCTC AGTATGGACC TGAAGCTGAC CAAGTGGGGC CAGAAGGATC ACAGCAGGTC 
ACAGTTAATC CTGATGCTTC GGAAATTCTG CAAGTCAAGA GAAGGAAAAT GCAGCTGTAC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NRAP cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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