HIDH cDNA ORF clone, Glycine max(soybean)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIDH
Entrez Gene ID	547489
Full Name	2-hydroxyisoflavanone dehydratase
Gene Type	protein-coding
Organism	Glycine max(soybean)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001250299.2	NP_001237228.1	2-hydroxyisoflavanone dehydratase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Pseudomonas syringae type III effector HopZ1 targets a host enzyme to suppress isoflavone biosynthesis and promote infection in soybean.
Zhou H, Lin J, Johnson A, Morgan RL, Zhong W, Ma W
Cell host & microbe9(3)177-186(2011 Mar)

Genome sequence of the palaeopolyploid soybean.
Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA
Nature463(7278)178-83(2010 Jan)

Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis.
Akashi T, Aoki T, Ayabe S
Plant physiology137(3)882-91(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Silencing 2-hydroxyisoflavanone dehydratase increases susceptibility to Pseudomonas syringae infection, supporting a role for 2-hydroxyisoflavanone dehydratase in innate immunity
Title: Pseudomonas syringae type III effector HopZ1 targets a host enzyme to suppress isoflavone biosynthesis and promote infection in soybean.

The following HIDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGl56361	NM_001250299.2 Latest version!	Glycine max 2-hydroxyisoflavanone dehydratase (HIDH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIDH ( NM_001250299.2 ) cDNA ORF clone, Glycine max(soybean) -> NP_001237228.1 Glycine max 2-hydroxyisoflavanone dehydratase (HIDH), mRNA.

Price & Availability↑

CloneID	OGl56361
Clone ID Related Accession (Same CDS sequence)	NM_001250299.2
Accession Version	NM_001250299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Glycine max(soybean)
Product	2-hydroxyisoflavanone dehydratase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT097440.1. On Sep 1, 2012 this sequence version replaced NM_001250299.1. ##Evidence-Data-START## Transcript is intronless :: BT097440.1, AK245894.1 [ECO:0000345] ##Evidence-Data-END##

ATGGCGAAGG AGATAGTGAA AGAGCTTCTT CCTCTAATTC GAGTGTACAA GGATGGCAGC 
GTGGAGCGTC TTCTAAGCTC TGAAAACGTG GCAGCCTCCC CTGAAGATCC CCAAACTGGA 
GTCTCATCCA AAGACATAGT CATCGCAGAC AACCCCTACG TCTCCGCTCG CATTTTCCTT 
CCCAAATCCC ACCACACTAA CAACAAACTC CCCATCTTCC TCTACTTCCA CGGTGGCGCC 
TTTTGCGTCG AATCCGCCTT CTCCTTTTTC GTCCACCGCT ATCTCAACAT CTTGGCCTCA 
GAAGCCAACA TAATAGCCAT CTCCGTCGAC TTCAGACTCC TCCCACACCA CCCTATCCCT 
GCTGCCTACG AAGACGGTTG GACCACCCTC AAATGGATTG CTTCCCACGC CAACAACACC 
AACACCACCA ACCCGGAGCC ATGGCTACTC AACCACGCCG ACTTCACCAA AGTCTACGTA 
GGAGGTGAAA CCAGCGGTGC TAACATCGCA CACAACCTGC TTTTGCGTGC AGGTAACGAA 
TCCCTCCCCG GGGATCTGAA AATATTGGGT GGATTACTAT GCTGCCCCTT CTTCTGGGGC 
TCGAAGCCAA TTGGGTCGGA GGCTGTTGAG GGGCACGAGC AGAGTTTGGC CATGAAGGTC 
TGGAACTTTG CCTGCCCTGA TGCCCCCGGT GGAATCGATA ACCCCTGGAT CAACCCCTGT 
GTTCCTGGGG CACCCTCTTT GGCCACTCTT GCCTGCTCTA AGTTGCTCGT TACTATCACT 
GGCAAAGACG AGTTCAGAGA CAGAGATATT CTCTACCACC ACACCGTTGA GCAAAGTGGC 
TGGCAAGGTG AACTTCAACT CTTTGATGCT GGCGATGAGG AGCATGCTTT CCAGCTCTTC 
AAGCCTGAGA CTCATCTTGC TAAAGCCATG ATCAAACGCT TGGCTTCTTT TCTGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001237228.1
CDS	10..969
Translation	MAKEIVKELLPLIRVYKDGSVERLLSSENVAASPEDPQTGVSSKDIVIADNPYVSARIFLPKSHHTNNKLPIFLYFHGGAFCVESAFSFFVHRYLNILASEANIIAISVDFRLLPHHPIPAAYEDGWTTLKWIASHANNTNTTNPEPWLLNHADFTKVYVGGETSGANIAHNLLLRAGNESLPGDLKILGGLLCCPFFWGSKPIGSEAVEGHEQSLAMKVWNFACPDAPGGIDNPWINPCVPGAPSLATLACSKLLVTITGKDEFRDRDILYHHTVEQSGWQGELQLFDAGDEEHAFQLFKPETHLAKAMIKRLASFLV

Target ORF information:

RefSeq Version	NM_001250299.2
Organism	Glycine max(soybean)
Definition	Glycine max 2-hydroxyisoflavanone dehydratase (HIDH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001250299.2

ORF Insert Sequence:

ATGGCGAAGG AGATAGTGAA AGAGCTTCTT CCTCTAATTC GAGTGTACAA GGATGGCAGC 
GTGGAGCGTC TTCTAAGCTC TGAAAACGTG GCAGCCTCCC CTGAAGATCC CCAAACTGGA 
GTCTCATCCA AAGACATAGT CATCGCAGAC AACCCCTACG TCTCCGCTCG CATTTTCCTT 
CCCAAATCCC ACCACACTAA CAACAAACTC CCCATCTTCC TCTACTTCCA CGGTGGCGCC 
TTTTGCGTCG AATCCGCCTT CTCCTTTTTC GTCCACCGCT ATCTCAACAT CTTGGCCTCA 
GAAGCCAACA TAATAGCCAT CTCCGTCGAC TTCAGACTCC TCCCACACCA CCCTATCCCT 
GCTGCCTACG AAGACGGTTG GACCACCCTC AAATGGATTG CTTCCCACGC CAACAACACC 
AACACCACCA ACCCGGAGCC ATGGCTACTC AACCACGCCG ACTTCACCAA AGTCTACGTA 
GGAGGTGAAA CCAGCGGTGC TAACATCGCA CACAACCTGC TTTTGCGTGC AGGTAACGAA 
TCCCTCCCCG GGGATCTGAA AATATTGGGT GGATTACTAT GCTGCCCCTT CTTCTGGGGC 
TCGAAGCCAA TTGGGTCGGA GGCTGTTGAG GGGCACGAGC AGAGTTTGGC CATGAAGGTC 
TGGAACTTTG CCTGCCCTGA TGCCCCCGGT GGAATCGATA ACCCCTGGAT CAACCCCTGT 
GTTCCTGGGG CACCCTCTTT GGCCACTCTT GCCTGCTCTA AGTTGCTCGT TACTATCACT 
GGCAAAGACG AGTTCAGAGA CAGAGATATT CTCTACCACC ACACCGTTGA GCAAAGTGGC 
TGGCAAGGTG AACTTCAACT CTTTGATGCT GGCGATGAGG AGCATGCTTT CCAGCTCTTC 
AAGCCTGAGA CTCATCTTGC TAAAGCCATG ATCAAACGCT TGGCTTCTTT TCTGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pseudomonas syringae type III effector HopZ1 targets a host enzyme to suppress isoflavone biosynthesis and promote infection in soybean.
Cell host & microbe9(3)177-186(2011 Mar)
Zhou H,Lin J,Johnson A,Morgan RL,Zhong W,Ma W

Genome sequence of the palaeopolyploid soybean.
Nature463(7278)178-83(2010 Jan)
Schmutz J,Cannon SB,Schlueter J,Ma J,Mitros T,Nelson W,Hyten DL,Song Q,Thelen JJ,Cheng J,Xu D,Hellsten U,May GD,Yu Y,Sakurai T,Umezawa T,Bhattacharyya MK,Sandhu D,Valliyodan B,Lindquist E,Peto M,Grant D,Shu S,Goodstein D,Barry K,Futrell-Griggs M,Abernathy B,Du J,Tian Z,Zhu L,Gill N,Joshi T,Libault M,Sethuraman A,Zhang XC,Shinozaki K,Nguyen HT,Wing RA,Cregan P,Specht J,Grimwood J,Rokhsar D,Stacey G,Shoemaker RC,Jackson SA

Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis.
Plant physiology137(3)882-91(2005 Mar)
Akashi T,Aoki T,Ayabe S