MYB176 cDNA ORF clone, Glycine max(soybean)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYB176
Entrez Gene ID	778059
Full Name	MYB transcription factor MYB176
Gene Type	protein-coding
Organism	Glycine max(soybean)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001249119.1	NP_001236048.1	MYB transcription factor MYB176
NM_001249119.2	NP_001236048.2	transcription factor MYB176

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide analysis of DWD proteins in soybean (Glycine max): Significance of Gm08DWD and GmMYB176 interaction in isoflavonoid biosynthesis.
Bian S, Li X, Mainali H, Chen L, Dhaubhadel S
PloS one12(6)e0178947(2017)

Soybean cyclophilin GmCYP1 interacts with an isoflavonoid regulator GmMYB176.
Mainali HR, Vadivel AK, Li X, Gijzen M, Dhaubhadel S
Scientific reports739550(2017 Jan)

14-3-3 proteins act as scaffolds for GmMYB62 and GmMYB176 and regulate their intracellular localization in soybean.
Li X, Dhaubhadel S
Plant signaling & behavior7(8)965-8(2012 Aug)

14-3-3 proteins regulate the intracellular localization of the transcriptional activator GmMYB176 and affect isoflavonoid synthesis in soybean.
Li X, Chen L, Dhaubhadel S
The Plant journal : for cell and molecular biology71(2)239-50(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Based on the functional investigation of Gm08DWD, we speculate that protein-protein interaction between Gm08DWD and GmMYB176 may lead to the degradation of GmMYB176 through CUL4-DDB1complex. [MYB176]
Title: Genome-wide analysis of DWD proteins in soybean (Glycine max): Significance of Gm08DWD and GmMYB176 interaction in isoflavonoid biosynthesis.

GmMYB62 is reported as a new R1 MYB client protein of soybean 14-3-3s that may function together with GmMYB176 for gene regulation in soybean.
Title: 14-3-3 proteins act as scaffolds for GmMYB62 and GmMYB176 and regulate their intracellular localization in soybean.

SGF14-GmMYB176 interaction regulates the intracellular localization of GmMYB176, thereby affecting isoflavonoid biosynthesis in soybean.
Title: 14-3-3 proteins regulate the intracellular localization of the transcriptional activator GmMYB176 and affect isoflavonoid synthesis in soybean.

GmMYB176 encodes an R1 MYB protein, and can trans-activate the CHS8 promoter. RNAi silencing of GmMYB176 in hairy roots resulted in reduced levels of isoflavonoids.
Title: A single-repeat MYB transcription factor, GmMYB176, regulates CHS8 gene expression and affects isoflavonoid biosynthesis in soybean.

The following MYB176 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYB176 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGl55258	NM_001249119.1 Latest version!	Glycine max MYB transcription factor MYB176 (MYB176), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OGl97322	NM_001249119.2 Latest version!	Glycine max transcription factor MYB176 (MYB176), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYB176 ( NM_001249119.1 ) cDNA ORF clone, Glycine max(soybean) -> NP_001236048.1 Glycine max MYB transcription factor MYB176 (MYB176), mRNA.

Price & Availability↑

CloneID	OGl55258
Clone ID Related Accession (Same CDS sequence)	NM_001249119.1
Accession Version	NM_001249119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-17
Organism	Glycine max(soybean)
Product	MYB transcription factor MYB176
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ822924.1. ##Evidence-Data-START## Transcript exon combination :: DQ822924.1, AK287076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00264986, SAMN00264988 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCGCG CCTCTTCCGC CGCCTCCGGC GAGATCATGC TGTTCGGGGT CAGAGTCGTC 
GTCGATTCGA TGAGGAAGAG CGTCAGCATG AACAACCTCT CACAGTACGA ACATCCTCTA 
GACGCCACCA CCACCACCAA CAACAAAGAC GCCGTCGCCG CCGGCTACGC CTCCGCCGAC 
GACGCCGCTC CTCAGAACTC CGGGCGCCAC CGCGAGCGCG AGCGAAAGCG AGGAGTTCCG 
TGGACGGAGG AAGAACACAA GTTGTTTTTG GTTGGATTGC AGAAAGTAGG GAAAGGTGAT 
TGGAGAGGAA TCTCCAAAAA CTACGTCAAA ACGCGAACTC CGACGCAGGT TGCCAGCCAT 
GCTCAGAAGT ACTTTCTCCG ACGAAGCAAC CTCAATCGCC GTCGCCGTAG ATCCAGCCTC 
TTTGACATCA CCACCGACAC GGTCTCTGCA ATTCCGATGG AGGAAGAACA GGTCCAGAAT 
CAAGACACCC TGTGTCATTC ACAACAACAA CCCGTGTTCC CTGCTGAAAC TAGCAAAATC 
AATGGGTTTC CAGCGATGCC AGTGTATCAG TTTGGGGTTG GTTCTTCTGG AGTGATTTCA 
GTCCAAGGTA CTGGAAACCC AATGGAAGAA CTCACTCTGG GACAAGGAAA CGTGGAAAAA 
CACAATGTGC CAAACAAGGC CTCTACAGTG TCTGGTATCA TCACCCCCGG TTCTTCTAGT 
TCTGCCATTG ACCCACCACC GACACTGTCC CTGGGGCTAT CCTTCTCATC TGACCAAAGA 
CAGACATCAT CAAGACATTC AGCTTTACAT GCCATGCAAT GTTTCAGCAA TGGAGATAGC 
ATCATTAGTG TTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001236048.1
CDS	120..977
Translation	MSRASSAASGEIMLFGVRVVVDSMRKSVSMNNLSQYEHPLDATTTTNNKDAVAAGYASADDAAPQNSGRHRERERKRGVPWTEEEHKLFLVGLQKVGKGDWRGISKNYVKTRTPTQVASHAQKYFLRRSNLNRRRRRSSLFDITTDTVSAIPMEEEQVQNQDTLCHSQQQPVFPAETSKINGFPAMPVYQFGVGSSGVISVQGTGNPMEELTLGQGNVEKHNVPNKASTVSGIITPGSSSSAIDPPPTLSLGLSFSSDQRQTSSRHSALHAMQCFSNGDSIISVA

Target ORF information:

RefSeq Version	NM_001249119.1
Organism	Glycine max(soybean)
Definition	Glycine max MYB transcription factor MYB176 (MYB176), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001249119.1

ORF Insert Sequence:

ATGTCTCGCG CCTCTTCCGC CGCCTCCGGC GAGATCATGC TGTTCGGGGT CAGAGTCGTC 
GTCGATTCGA TGAGGAAGAG CGTCAGCATG AACAACCTCT CACAGTACGA ACATCCTCTA 
GACGCCACCA CCACCACCAA CAACAAAGAC GCCGTCGCCG CCGGCTACGC CTCCGCCGAC 
GACGCCGCTC CTCAGAACTC CGGGCGCCAC CGCGAGCGCG AGCGAAAGCG AGGAGTTCCG 
TGGACGGAGG AAGAACACAA GTTGTTTTTG GTTGGATTGC AGAAAGTAGG GAAAGGTGAT 
TGGAGAGGAA TCTCCAAAAA CTACGTCAAA ACGCGAACTC CGACGCAGGT TGCCAGCCAT 
GCTCAGAAGT ACTTTCTCCG ACGAAGCAAC CTCAATCGCC GTCGCCGTAG ATCCAGCCTC 
TTTGACATCA CCACCGACAC GGTCTCTGCA ATTCCGATGG AGGAAGAACA GGTCCAGAAT 
CAAGACACCC TGTGTCATTC ACAACAACAA CCCGTGTTCC CTGCTGAAAC TAGCAAAATC 
AATGGGTTTC CAGCGATGCC AGTGTATCAG TTTGGGGTTG GTTCTTCTGG AGTGATTTCA 
GTCCAAGGTA CTGGAAACCC AATGGAAGAA CTCACTCTGG GACAAGGAAA CGTGGAAAAA 
CACAATGTGC CAAACAAGGC CTCTACAGTG TCTGGTATCA TCACCCCCGG TTCTTCTAGT 
TCTGCCATTG ACCCACCACC GACACTGTCC CTGGGGCTAT CCTTCTCATC TGACCAAAGA 
CAGACATCAT CAAGACATTC AGCTTTACAT GCCATGCAAT GTTTCAGCAA TGGAGATAGC 
ATCATTAGTG TTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYB176 ( NM_001249119.2 ) cDNA ORF clone, Glycine max(soybean) -> NP_001236048.2 Glycine max transcription factor MYB176 (MYB176), mRNA.

Price & Availability↑

CloneID	OGl97322
Clone ID Related Accession (Same CDS sequence)	NM_001249119.2
Accession Version	NM_001249119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-26
Organism	Glycine max(soybean)
Product	transcription factor MYB176
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from ACUP03002809.1. On Jun 27, 2019 this sequence version replaced NM_001249119.1. ##Evidence-Data-START## Transcript exon combination :: DQ822924.1, AK287076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00264986, SAMN00264988 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCGCG CCTCTTCCGC CGCCTCCGGC GAGATCATGC TGTTCGGGGT CAGAGTCGTC 
GTCGATTCGA TGAGGAAGAG CGTCAGCATG AACAACCTCT CACAGTACGA ACATCCTTTA 
GACGCCACCA CCACCAACAA CAACAAAGAC GCCGTCGCCG CCGGCTACGC CTCCGCCGAC 
GACGCCGCTC CTCAGAACTC CGGGCGCCAC CGCGAGCGCG AGCGAAAGCG AGGAGTTCCG 
TGGACGGAGG AAGAACACAA GTTGTTTTTG GTTGGATTGC AGAAAGTAGG GAAAGGTGAT 
TGGAGAGGAA TCTCCAAAAA CTACGTCAAA ACGCGAACTC CGACGCAGGT TGCCAGCCAT 
GCTCAGAAGT ACTTTCTCCG ACGAAGCAAC CTCAATCGCC GTCGCCGTAG ATCCAGCCTC 
TTTGACATCA CCACCGACAC GGTCTCTGCA ATTCCGATGG AGGAAGAACA GGTCCAGAAT 
CAAGACACCC TGTGTCATTC ACAACAACAA CCCGTGTTCC CTGCTGAAAC TAGCAAAATC 
AATGGGTTTC CAGCGATGCC AGTGTATCAG TTTGGGGTTG GTTCTTCTGG AGTGATTTCA 
GTCCAAGGTA CTGGAAACCC AATGGAAGAA CTCACTCTGG GACAAGGAAA CGTGGAAAAA 
CACAATGTGC CAAACAAGGC CTCTACAGTG TCTGGTATCA TCACCCCCGG TTCTTCTAGT 
TCTGCCATTG ACCCACCACC GACACTGTCC CTGGGGCTAT CCTTCTCATC TGACCAAAGA 
CAGACATCAT CAAGACATTC AGCTTTACAT GCCATGCAAT GTTTCAGCAA TGGAGATAGC 
ATCATTAGTG TTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001236048.2
CDS	134..991
Translation	MSRASSAASGEIMLFGVRVVVDSMRKSVSMNNLSQYEHPLDATTTNNNKDAVAAGYASADDAAPQNSGRHRERERKRGVPWTEEEHKLFLVGLQKVGKGDWRGISKNYVKTRTPTQVASHAQKYFLRRSNLNRRRRRSSLFDITTDTVSAIPMEEEQVQNQDTLCHSQQQPVFPAETSKINGFPAMPVYQFGVGSSGVISVQGTGNPMEELTLGQGNVEKHNVPNKASTVSGIITPGSSSSAIDPPPTLSLGLSFSSDQRQTSSRHSALHAMQCFSNGDSIISVA

Target ORF information:

RefSeq Version	NM_001249119.2
Organism	Glycine max(soybean)
Definition	Glycine max transcription factor MYB176 (MYB176), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001249119.2

ORF Insert Sequence:

ATGTCTCGCG CCTCTTCCGC CGCCTCCGGC GAGATCATGC TGTTCGGGGT CAGAGTCGTC 
GTCGATTCGA TGAGGAAGAG CGTCAGCATG AACAACCTCT CACAGTACGA ACATCCTTTA 
GACGCCACCA CCACCAACAA CAACAAAGAC GCCGTCGCCG CCGGCTACGC CTCCGCCGAC 
GACGCCGCTC CTCAGAACTC CGGGCGCCAC CGCGAGCGCG AGCGAAAGCG AGGAGTTCCG 
TGGACGGAGG AAGAACACAA GTTGTTTTTG GTTGGATTGC AGAAAGTAGG GAAAGGTGAT 
TGGAGAGGAA TCTCCAAAAA CTACGTCAAA ACGCGAACTC CGACGCAGGT TGCCAGCCAT 
GCTCAGAAGT ACTTTCTCCG ACGAAGCAAC CTCAATCGCC GTCGCCGTAG ATCCAGCCTC 
TTTGACATCA CCACCGACAC GGTCTCTGCA ATTCCGATGG AGGAAGAACA GGTCCAGAAT 
CAAGACACCC TGTGTCATTC ACAACAACAA CCCGTGTTCC CTGCTGAAAC TAGCAAAATC 
AATGGGTTTC CAGCGATGCC AGTGTATCAG TTTGGGGTTG GTTCTTCTGG AGTGATTTCA 
GTCCAAGGTA CTGGAAACCC AATGGAAGAA CTCACTCTGG GACAAGGAAA CGTGGAAAAA 
CACAATGTGC CAAACAAGGC CTCTACAGTG TCTGGTATCA TCACCCCCGG TTCTTCTAGT 
TCTGCCATTG ACCCACCACC GACACTGTCC CTGGGGCTAT CCTTCTCATC TGACCAAAGA 
CAGACATCAT CAAGACATTC AGCTTTACAT GCCATGCAAT GTTTCAGCAA TGGAGATAGC 
ATCATTAGTG TTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide analysis of DWD proteins in soybean (Glycine max): Significance of Gm08DWD and GmMYB176 interaction in isoflavonoid biosynthesis.
PloS one12(6)e0178947(2017)
Bian S,Li X,Mainali H,Chen L,Dhaubhadel S

Soybean cyclophilin GmCYP1 interacts with an isoflavonoid regulator GmMYB176.
Scientific reports739550(2017 Jan)
Mainali HR,Vadivel AK,Li X,Gijzen M,Dhaubhadel S

14-3-3 proteins act as scaffolds for GmMYB62 and GmMYB176 and regulate their intracellular localization in soybean.
Plant signaling & behavior7(8)965-8(2012 Aug)
Li X,Dhaubhadel S

14-3-3 proteins regulate the intracellular localization of the transcriptional activator GmMYB176 and affect isoflavonoid synthesis in soybean.
The Plant journal : for cell and molecular biology71(2)239-50(2012 Jul)
Li X,Chen L,Dhaubhadel S