MSLN cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following MSLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu21046 NM_005823.6
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu21046 NM_001177355.3
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17473 NM_013404.4
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu17473 XM_011522348.2
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu17473 XM_017022857.1
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu48743 XM_005255034.4
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu63786 XM_017022858.1
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu85806 XM_017022853.1
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu85807 XM_017022854.1
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu85808 XM_017022855.1
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu85809 XM_017022856.1
Latest version!
Homo sapiens mesothelin (MSLN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu21046 NM_001177355.2 Homo sapiens mesothelin (MSLN), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu21046 NM_005823.5 Homo sapiens mesothelin (MSLN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu21046
    Clone ID Related Accession (Same CDS sequence) NM_001177355.3 , NM_005823.6 , NM_001177355.2 , NM_005823.5
    Accession Version NM_001177355.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product mesothelin isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL031258.12, AY743922.1, BC009272.2 and BC003512.1. On May 31, 2019 this sequence version replaced NM_001177355.2. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009272.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GTGGCCACCC TGATCGACCG CTTTGTGAAG
    GGAAGGGGCC AGCTAGACAA AGACACCCTA GACACCCTGA CCGCCTTCTA CCCTGGGTAC
    CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC
    AGGCCCCAGG ACCTGGACAC GTGTGACCCA AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC
    CGCCTTGCTT TCCAGAACAT GAACGGGTCC GAATACTTCG TGAAGATCCA GTCCTTCCTG
    GGTGGGGCCC CCACGGAGGA TTTGAAGGCG CTCAGTCAGC AGAATGTGAG CATGGACTTG
    GCCACGTTCA TGAAGCTGCG GACGGATGCG GTGCTGCCGT TGACTGTGGC TGAGGTGCAG
    AAACTTCTGG GACCCCACGT GGAGGGCCTG AAGGCGGAGG AGCGGCACCG CCCGGTGCGG
    GACTGGATCC TACGGCAGCG GCAGGACGAC CTGGACACGC TGGGGCTGGG GCTACAGGGC
    GGCATCCCCA ACGGCTACCT GGTCCTAGAC CTCAGCATGC AAGAGGCCCT CTCGGGGACG
    CCCTGCCTCC TAGGACCTGG ACCTGTTCTC ACCGTCCTGG CACTGCTCCT AGCCTCCACC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001170826.1
    CDS140..2008
    Translation

    Target ORF information:

    RefSeq Version NM_001177355.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177355.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GTGGCCACCC TGATCGACCG CTTTGTGAAG
    GGAAGGGGCC AGCTAGACAA AGACACCCTA GACACCCTGA CCGCCTTCTA CCCTGGGTAC
    CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC
    AGGCCCCAGG ACCTGGACAC GTGTGACCCA AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC
    CGCCTTGCTT TCCAGAACAT GAACGGGTCC GAATACTTCG TGAAGATCCA GTCCTTCCTG
    GGTGGGGCCC CCACGGAGGA TTTGAAGGCG CTCAGTCAGC AGAATGTGAG CATGGACTTG
    GCCACGTTCA TGAAGCTGCG GACGGATGCG GTGCTGCCGT TGACTGTGGC TGAGGTGCAG
    AAACTTCTGG GACCCCACGT GGAGGGCCTG AAGGCGGAGG AGCGGCACCG CCCGGTGCGG
    GACTGGATCC TACGGCAGCG GCAGGACGAC CTGGACACGC TGGGGCTGGG GCTACAGGGC
    GGCATCCCCA ACGGCTACCT GGTCCTAGAC CTCAGCATGC AAGAGGCCCT CTCGGGGACG
    CCCTGCCTCC TAGGACCTGG ACCTGTTCTC ACCGTCCTGG CACTGCTCCT AGCCTCCACC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21046
    Clone ID Related Accession (Same CDS sequence) NM_001177355.3 , NM_005823.6 , NM_001177355.2 , NM_005823.5
    Accession Version NM_005823.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product mesothelin isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL031258.12, AY743922.1 and BC003512.1. On Nov 23, 2018 this sequence version replaced NM_005823.5. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Both variants 1 and 3 encode the same isoform isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY743922.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000545450.7/ ENSP00000442965.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GTGGCCACCC TGATCGACCG CTTTGTGAAG
    GGAAGGGGCC AGCTAGACAA AGACACCCTA GACACCCTGA CCGCCTTCTA CCCTGGGTAC
    CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC
    AGGCCCCAGG ACCTGGACAC GTGTGACCCA AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC
    CGCCTTGCTT TCCAGAACAT GAACGGGTCC GAATACTTCG TGAAGATCCA GTCCTTCCTG
    GGTGGGGCCC CCACGGAGGA TTTGAAGGCG CTCAGTCAGC AGAATGTGAG CATGGACTTG
    GCCACGTTCA TGAAGCTGCG GACGGATGCG GTGCTGCCGT TGACTGTGGC TGAGGTGCAG
    AAACTTCTGG GACCCCACGT GGAGGGCCTG AAGGCGGAGG AGCGGCACCG CCCGGTGCGG
    GACTGGATCC TACGGCAGCG GCAGGACGAC CTGGACACGC TGGGGCTGGG GCTACAGGGC
    GGCATCCCCA ACGGCTACCT GGTCCTAGAC CTCAGCATGC AAGAGGCCCT CTCGGGGACG
    CCCTGCCTCC TAGGACCTGG ACCTGTTCTC ACCGTCCTGG CACTGCTCCT AGCCTCCACC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005814.2
    CDS200..2068
    Translation

    Target ORF information:

    RefSeq Version NM_005823.6
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005823.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GTGGCCACCC TGATCGACCG CTTTGTGAAG
    GGAAGGGGCC AGCTAGACAA AGACACCCTA GACACCCTGA CCGCCTTCTA CCCTGGGTAC
    CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC
    AGGCCCCAGG ACCTGGACAC GTGTGACCCA AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC
    CGCCTTGCTT TCCAGAACAT GAACGGGTCC GAATACTTCG TGAAGATCCA GTCCTTCCTG
    GGTGGGGCCC CCACGGAGGA TTTGAAGGCG CTCAGTCAGC AGAATGTGAG CATGGACTTG
    GCCACGTTCA TGAAGCTGCG GACGGATGCG GTGCTGCCGT TGACTGTGGC TGAGGTGCAG
    AAACTTCTGG GACCCCACGT GGAGGGCCTG AAGGCGGAGG AGCGGCACCG CCCGGTGCGG
    GACTGGATCC TACGGCAGCG GCAGGACGAC CTGGACACGC TGGGGCTGGG GCTACAGGGC
    GGCATCCCCA ACGGCTACCT GGTCCTAGAC CTCAGCATGC AAGAGGCCCT CTCGGGGACG
    CCCTGCCTCC TAGGACCTGG ACCTGTTCTC ACCGTCCTGG CACTGCTCCT AGCCTCCACC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21046
    Clone ID Related Accession (Same CDS sequence) NM_001177355.3 , NM_005823.6 , NM_001177355.2 , NM_005823.5
    Accession Version NM_001177355.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product mesothelin isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY743922.1, BC009272.2, AL031258.12 and BC003512.1. On Feb 26, 2016 this sequence version replaced NM_001177355.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009272.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GTGGCCACCC TGATCGACCG CTTTGTGAAG
    GGAAGGGGCC AGCTAGACAA AGACACCCTA GACACCCTGA CCGCCTTCTA CCCTGGGTAC
    CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC
    AGGCCCCAGG ACCTGGACAC GTGTGACCCA AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC
    CGCCTTGCTT TCCAGAACAT GAACGGGTCC GAATACTTCG TGAAGATCCA GTCCTTCCTG
    GGTGGGGCCC CCACGGAGGA TTTGAAGGCG CTCAGTCAGC AGAATGTGAG CATGGACTTG
    GCCACGTTCA TGAAGCTGCG GACGGATGCG GTGCTGCCGT TGACTGTGGC TGAGGTGCAG
    AAACTTCTGG GACCCCACGT GGAGGGCCTG AAGGCGGAGG AGCGGCACCG CCCGGTGCGG
    GACTGGATCC TACGGCAGCG GCAGGACGAC CTGGACACGC TGGGGCTGGG GCTACAGGGC
    GGCATCCCCA ACGGCTACCT GGTCCTAGAC CTCAGCATGC AAGAGGCCCT CTCGGGGACG
    CCCTGCCTCC TAGGACCTGG ACCTGTTCTC ACCGTCCTGG CACTGCTCCT AGCCTCCACC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001170826.1
    CDS109..1977
    Translation

    Target ORF information:

    RefSeq Version NM_001177355.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177355.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GTGGCCACCC TGATCGACCG CTTTGTGAAG
    GGAAGGGGCC AGCTAGACAA AGACACCCTA GACACCCTGA CCGCCTTCTA CCCTGGGTAC
    CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC
    AGGCCCCAGG ACCTGGACAC GTGTGACCCA AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC
    CGCCTTGCTT TCCAGAACAT GAACGGGTCC GAATACTTCG TGAAGATCCA GTCCTTCCTG
    GGTGGGGCCC CCACGGAGGA TTTGAAGGCG CTCAGTCAGC AGAATGTGAG CATGGACTTG
    GCCACGTTCA TGAAGCTGCG GACGGATGCG GTGCTGCCGT TGACTGTGGC TGAGGTGCAG
    AAACTTCTGG GACCCCACGT GGAGGGCCTG AAGGCGGAGG AGCGGCACCG CCCGGTGCGG
    GACTGGATCC TACGGCAGCG GCAGGACGAC CTGGACACGC TGGGGCTGGG GCTACAGGGC
    GGCATCCCCA ACGGCTACCT GGTCCTAGAC CTCAGCATGC AAGAGGCCCT CTCGGGGACG
    CCCTGCCTCC TAGGACCTGG ACCTGTTCTC ACCGTCCTGG CACTGCTCCT AGCCTCCACC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21046
    Clone ID Related Accession (Same CDS sequence) NM_001177355.3 , NM_005823.6 , NM_001177355.2 , NM_005823.5
    Accession Version NM_005823.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-10
    Organism Homo sapiens(human)
    Product mesothelin isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY743922.1, AL031258.12 and BC003512.1. On Apr 7, 2010 this sequence version replaced NM_005823.4. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Both variants 1 and 3 encode the same isoform isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY743922.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GTGGCCACCC TGATCGACCG CTTTGTGAAG
    GGAAGGGGCC AGCTAGACAA AGACACCCTA GACACCCTGA CCGCCTTCTA CCCTGGGTAC
    CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC
    AGGCCCCAGG ACCTGGACAC GTGTGACCCA AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC
    CGCCTTGCTT TCCAGAACAT GAACGGGTCC GAATACTTCG TGAAGATCCA GTCCTTCCTG
    GGTGGGGCCC CCACGGAGGA TTTGAAGGCG CTCAGTCAGC AGAATGTGAG CATGGACTTG
    GCCACGTTCA TGAAGCTGCG GACGGATGCG GTGCTGCCGT TGACTGTGGC TGAGGTGCAG
    AAACTTCTGG GACCCCACGT GGAGGGCCTG AAGGCGGAGG AGCGGCACCG CCCGGTGCGG
    GACTGGATCC TACGGCAGCG GCAGGACGAC CTGGACACGC TGGGGCTGGG GCTACAGGGC
    GGCATCCCCA ACGGCTACCT GGTCCTAGAC CTCAGCATGC AAGAGGCCCT CTCGGGGACG
    CCCTGCCTCC TAGGACCTGG ACCTGTTCTC ACCGTCCTGG CACTGCTCCT AGCCTCCACC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005814.2
    CDS169..2037
    Translation

    Target ORF information:

    RefSeq Version NM_005823.5
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005823.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GTGGCCACCC TGATCGACCG CTTTGTGAAG
    GGAAGGGGCC AGCTAGACAA AGACACCCTA GACACCCTGA CCGCCTTCTA CCCTGGGTAC
    CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC
    AGGCCCCAGG ACCTGGACAC GTGTGACCCA AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC
    CGCCTTGCTT TCCAGAACAT GAACGGGTCC GAATACTTCG TGAAGATCCA GTCCTTCCTG
    GGTGGGGCCC CCACGGAGGA TTTGAAGGCG CTCAGTCAGC AGAATGTGAG CATGGACTTG
    GCCACGTTCA TGAAGCTGCG GACGGATGCG GTGCTGCCGT TGACTGTGGC TGAGGTGCAG
    AAACTTCTGG GACCCCACGT GGAGGGCCTG AAGGCGGAGG AGCGGCACCG CCCGGTGCGG
    GACTGGATCC TACGGCAGCG GCAGGACGAC CTGGACACGC TGGGGCTGGG GCTACAGGGC
    GGCATCCCCA ACGGCTACCT GGTCCTAGAC CTCAGCATGC AAGAGGCCCT CTCGGGGACG
    CCCTGCCTCC TAGGACCTGG ACCTGTTCTC ACCGTCCTGG CACTGCTCCT AGCCTCCACC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17473
    Clone ID Related Accession (Same CDS sequence) XM_017022857.1 , XM_011522348.2 , NM_013404.4
    Accession Version NM_013404.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product mesothelin isoform 2 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL545401.3, D49441.1, CB266931.1, BC009272.2 and BC003512.1. On Apr 7, 2010 this sequence version replaced NM_013404.3. Transcript Variant: This transcript variant (2) uses an alternate splice site in the coding region, compared to variant 1. Variant 2 encodes isoform 2 which is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U40434.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GCTCCTCGGC GGCCCCTCCC ACAGGTGGCC
    ACCCTGATCG ACCGCTTTGT GAAGGGAAGG GGCCAGCTAG ACAAAGACAC CCTAGACACC
    CTGACCGCCT TCTACCCTGG GTACCTGTGC TCCCTCAGCC CCGAGGAGCT GAGCTCCGTG
    CCCCCCAGCA GCATCTGGGC GGTCAGGCCC CAGGACCTGG ACACGTGTGA CCCAAGGCAG
    CTGGACGTCC TCTATCCCAA GGCCCGCCTT GCTTTCCAGA ACATGAACGG GTCCGAATAC
    TTCGTGAAGA TCCAGTCCTT CCTGGGTGGG GCCCCCACGG AGGATTTGAA GGCGCTCAGT
    CAGCAGAATG TGAGCATGGA CTTGGCCACG TTCATGAAGC TGCGGACGGA TGCGGTGCTG
    CCGTTGACTG TGGCTGAGGT GCAGAAACTT CTGGGACCCC ACGTGGAGGG CCTGAAGGCG
    GAGGAGCGGC ACCGCCCGGT GCGGGACTGG ATCCTACGGC AGCGGCAGGA CGACCTGGAC
    ACGCTGGGGC TGGGGCTACA GGGCGGCATC CCCAACGGCT ACCTGGTCCT AGACCTCAGC
    ATGCAAGAGG CCCTCTCGGG GACGCCCTGC CTCCTAGGAC CTGGACCTGT TCTCACCGTC
    CTGGCACTGC TCCTAGCCTC CACCCTGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_037536.2
    CDS112..2004
    Translation

    Target ORF information:

    RefSeq Version NM_013404.4
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_013404.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GCTCCTCGGC GGCCCCTCCC ACAGGTGGCC
    ACCCTGATCG ACCGCTTTGT GAAGGGAAGG GGCCAGCTAG ACAAAGACAC CCTAGACACC
    CTGACCGCCT TCTACCCTGG GTACCTGTGC TCCCTCAGCC CCGAGGAGCT GAGCTCCGTG
    CCCCCCAGCA GCATCTGGGC GGTCAGGCCC CAGGACCTGG ACACGTGTGA CCCAAGGCAG
    CTGGACGTCC TCTATCCCAA GGCCCGCCTT GCTTTCCAGA ACATGAACGG GTCCGAATAC
    TTCGTGAAGA TCCAGTCCTT CCTGGGTGGG GCCCCCACGG AGGATTTGAA GGCGCTCAGT
    CAGCAGAATG TGAGCATGGA CTTGGCCACG TTCATGAAGC TGCGGACGGA TGCGGTGCTG
    CCGTTGACTG TGGCTGAGGT GCAGAAACTT CTGGGACCCC ACGTGGAGGG CCTGAAGGCG
    GAGGAGCGGC ACCGCCCGGT GCGGGACTGG ATCCTACGGC AGCGGCAGGA CGACCTGGAC
    ACGCTGGGGC TGGGGCTACA GGGCGGCATC CCCAACGGCT ACCTGGTCCT AGACCTCAGC
    ATGCAAGAGG CCCTCTCGGG GACGCCCTGC CTCCTAGGAC CTGGACCTGT TCTCACCGTC
    CTGGCACTGC TCCTAGCCTC CACCCTGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17473
    Clone ID Related Accession (Same CDS sequence) XM_017022857.1 , XM_011522348.2 , NM_013404.4
    Accession Version XM_017022857.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mesothelin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GCTCCTCGGC GGCCCCTCCC ACAGGTGGCC
    ACCCTGATCG ACCGCTTTGT GAAGGGAAGG GGCCAGCTAG ACAAAGACAC CCTAGACACC
    CTGACCGCCT TCTACCCTGG GTACCTGTGC TCCCTCAGCC CCGAGGAGCT GAGCTCCGTG
    CCCCCCAGCA GCATCTGGGC GGTCAGGCCC CAGGACCTGG ACACGTGTGA CCCAAGGCAG
    CTGGACGTCC TCTATCCCAA GGCCCGCCTT GCTTTCCAGA ACATGAACGG GTCCGAATAC
    TTCGTGAAGA TCCAGTCCTT CCTGGGTGGG GCCCCCACGG AGGATTTGAA GGCGCTCAGT
    CAGCAGAATG TGAGCATGGA CTTGGCCACG TTCATGAAGC TGCGGACGGA TGCGGTGCTG
    CCGTTGACTG TGGCTGAGGT GCAGAAACTT CTGGGACCCC ACGTGGAGGG CCTGAAGGCG
    GAGGAGCGGC ACCGCCCGGT GCGGGACTGG ATCCTACGGC AGCGGCAGGA CGACCTGGAC
    ACGCTGGGGC TGGGGCTACA GGGCGGCATC CCCAACGGCT ACCTGGTCCT AGACCTCAGC
    ATGCAAGAGG CCCTCTCGGG GACGCCCTGC CTCCTAGGAC CTGGACCTGT TCTCACCGTC
    CTGGCACTGC TCCTAGCCTC CACCCTGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878346.1
    CDS259..2151
    Translation

    Target ORF information:

    RefSeq Version XM_017022857.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022857.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GCTCCTCGGC GGCCCCTCCC ACAGGTGGCC
    ACCCTGATCG ACCGCTTTGT GAAGGGAAGG GGCCAGCTAG ACAAAGACAC CCTAGACACC
    CTGACCGCCT TCTACCCTGG GTACCTGTGC TCCCTCAGCC CCGAGGAGCT GAGCTCCGTG
    CCCCCCAGCA GCATCTGGGC GGTCAGGCCC CAGGACCTGG ACACGTGTGA CCCAAGGCAG
    CTGGACGTCC TCTATCCCAA GGCCCGCCTT GCTTTCCAGA ACATGAACGG GTCCGAATAC
    TTCGTGAAGA TCCAGTCCTT CCTGGGTGGG GCCCCCACGG AGGATTTGAA GGCGCTCAGT
    CAGCAGAATG TGAGCATGGA CTTGGCCACG TTCATGAAGC TGCGGACGGA TGCGGTGCTG
    CCGTTGACTG TGGCTGAGGT GCAGAAACTT CTGGGACCCC ACGTGGAGGG CCTGAAGGCG
    GAGGAGCGGC ACCGCCCGGT GCGGGACTGG ATCCTACGGC AGCGGCAGGA CGACCTGGAC
    ACGCTGGGGC TGGGGCTACA GGGCGGCATC CCCAACGGCT ACCTGGTCCT AGACCTCAGC
    ATGCAAGAGG CCCTCTCGGG GACGCCCTGC CTCCTAGGAC CTGGACCTGT TCTCACCGTC
    CTGGCACTGC TCCTAGCCTC CACCCTGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17473
    Clone ID Related Accession (Same CDS sequence) XM_017022857.1 , XM_011522348.2 , NM_013404.4
    Accession Version XM_011522348.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mesothelin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GCTCCTCGGC GGCCCCTCCC ACAGGTGGCC
    ACCCTGATCG ACCGCTTTGT GAAGGGAAGG GGCCAGCTAG ACAAAGACAC CCTAGACACC
    CTGACCGCCT TCTACCCTGG GTACCTGTGC TCCCTCAGCC CCGAGGAGCT GAGCTCCGTG
    CCCCCCAGCA GCATCTGGGC GGTCAGGCCC CAGGACCTGG ACACGTGTGA CCCAAGGCAG
    CTGGACGTCC TCTATCCCAA GGCCCGCCTT GCTTTCCAGA ACATGAACGG GTCCGAATAC
    TTCGTGAAGA TCCAGTCCTT CCTGGGTGGG GCCCCCACGG AGGATTTGAA GGCGCTCAGT
    CAGCAGAATG TGAGCATGGA CTTGGCCACG TTCATGAAGC TGCGGACGGA TGCGGTGCTG
    CCGTTGACTG TGGCTGAGGT GCAGAAACTT CTGGGACCCC ACGTGGAGGG CCTGAAGGCG
    GAGGAGCGGC ACCGCCCGGT GCGGGACTGG ATCCTACGGC AGCGGCAGGA CGACCTGGAC
    ACGCTGGGGC TGGGGCTACA GGGCGGCATC CCCAACGGCT ACCTGGTCCT AGACCTCAGC
    ATGCAAGAGG CCCTCTCGGG GACGCCCTGC CTCCTAGGAC CTGGACCTGT TCTCACCGTC
    CTGGCACTGC TCCTAGCCTC CACCCTGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520650.1
    CDS182..2074
    Translation

    Target ORF information:

    RefSeq Version XM_011522348.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011522348.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG AGGCTGCGCC CCTGGACGGA GTCCTGGCCA ACCCACCTAA CATTTCCAGC
    CTCTCCCCTC GCCAACTCCT TGGCTTCCCG TGTGCGGAGG TGTCCGGCCT GAGCACGGAG
    CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA CAGAAGAATG TCAAGCTCTC AACAGAGCAG
    CTGCGCTGTC TGGCTCACCG GCTCTCTGAG CCCCCCGAGG ACCTGGACGC CCTCCCATTG
    GACCTGCTGC TATTCCTCAA CCCAGATGCG TTCTCGGGGC CCCAGGCCTG CACCCGTTTC
    TTCTCCCGCA TCACGAAGGC CAATGTGGAC CTGCTCCCGA GGGGGGCTCC CGAGCGACAG
    CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT
    GATGTGCGGG CTCTGGGAGG CCTGGCTTGC GACCTGCCTG GGCGCTTTGT GGCCGAGTCG
    GCCGAAGTGC TGCTACCCCG GCTGGTGAGC TGCCCGGGAC CCCTGGACCA GGACCAGCAG
    GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG GGACCCCCCT ACGGCCCCCC GTCGACATGG
    TCTGTCTCCA CGATGGACGC TCTGCGGGGC CTGCTGCCCG TGCTGGGCCA GCCCATCATC
    CGCAGCATCC CGCAGGGCAT CGTGGCCGCG TGGCGGCAAC GCTCCTCTCG GGACCCATCC
    TGGCGGCAGC CTGAACGGAC CATCCTCCGG CCGCGGTTCC GGCGGGAAGT GGAGAAGACA
    GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG ATAGACGAGA GCCTCATCTT CTACAAGAAG
    TGGGAGCTGG AAGCCTGCGT GGATGCGGCC CTGCTGGCCA CCCAGATGGA CCGCGTGAAC
    GCCATCCCCT TCACCTACGA GCAGCTGGAC GTCCTAAAGC ATAAACTGGA TGAGCTCTAC
    CCACAAGGTT ACCCCGAGTC TGTGATCCAG CACCTGGGCT ACCTCTTCCT CAAGATGAGC
    CCTGAGGACA TTCGCAAGTG GAATGTGACG TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA
    GTCAACAAAG GGCACGAAAT GAGTCCTCAG GCTCCTCGGC GGCCCCTCCC ACAGGTGGCC
    ACCCTGATCG ACCGCTTTGT GAAGGGAAGG GGCCAGCTAG ACAAAGACAC CCTAGACACC
    CTGACCGCCT TCTACCCTGG GTACCTGTGC TCCCTCAGCC CCGAGGAGCT GAGCTCCGTG
    CCCCCCAGCA GCATCTGGGC GGTCAGGCCC CAGGACCTGG ACACGTGTGA CCCAAGGCAG
    CTGGACGTCC TCTATCCCAA GGCCCGCCTT GCTTTCCAGA ACATGAACGG GTCCGAATAC
    TTCGTGAAGA TCCAGTCCTT CCTGGGTGGG GCCCCCACGG AGGATTTGAA GGCGCTCAGT
    CAGCAGAATG TGAGCATGGA CTTGGCCACG TTCATGAAGC TGCGGACGGA TGCGGTGCTG
    CCGTTGACTG TGGCTGAGGT GCAGAAACTT CTGGGACCCC ACGTGGAGGG CCTGAAGGCG
    GAGGAGCGGC ACCGCCCGGT GCGGGACTGG ATCCTACGGC AGCGGCAGGA CGACCTGGAC
    ACGCTGGGGC TGGGGCTACA GGGCGGCATC CCCAACGGCT ACCTGGTCCT AGACCTCAGC
    ATGCAAGAGG CCCTCTCGGG GACGCCCTGC CTCCTAGGAC CTGGACCTGT TCTCACCGTC
    CTGGCACTGC TCCTAGCCTC CACCCTGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48743
    Clone ID Related Accession (Same CDS sequence) XM_005255034.4
    Accession Version XM_005255034.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mesothelin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG CTGCGCCCCT GGACGGAGTC CTGGCCAACC CACCTAACAT TTCCAGCCTC
    TCCCCTCGCC AACTCCTTGG CTTCCCGTGT GCGGAGGTGT CCGGCCTGAG CACGGAGCGT
    GTCCGGGAGC TGGCTGTGGC CTTGGCACAG AAGAATGTCA AGCTCTCAAC AGAGCAGCTG
    CGCTGTCTGG CTCACCGGCT CTCTGAGCCC CCCGAGGACC TGGACGCCCT CCCATTGGAC
    CTGCTGCTAT TCCTCAACCC AGATGCGTTC TCGGGGCCCC AGGCCTGCAC CCGTTTCTTC
    TCCCGCATCA CGAAGGCCAA TGTGGACCTG CTCCCGAGGG GGGCTCCCGA GCGACAGCGG
    CTGCTGCCTG CGGCTCTGGC CTGCTGGGGT GTGCGGGGGT CTCTGCTGAG CGAGGCTGAT
    GTGCGGGCTC TGGGAGGCCT GGCTTGCGAC CTGCCTGGGC GCTTTGTGGC CGAGTCGGCC
    GAAGTGCTGC TACCCCGGCT GGTGAGCTGC CCGGGACCCC TGGACCAGGA CCAGCAGGAG
    GCAGCCAGGG CGGCTCTGCA GGGCGGGGGA CCCCCCTACG GCCCCCCGTC GACATGGTCT
    GTCTCCACGA TGGACGCTCT GCGGGGCCTG CTGCCCGTGC TGGGCCAGCC CATCATCCGC
    AGCATCCCGC AGGGCATCGT GGCCGCGTGG CGGCAACGCT CCTCTCGGGA CCCATCCTGG
    CGGCAGCCTG AACGGACCAT CCTCCGGCCG CGGTTCCGGC GGGAAGTGGA GAAGACAGCC
    TGTCCTTCAG GCAAGAAGGC CCGCGAGATA GACGAGAGCC TCATCTTCTA CAAGAAGTGG
    GAGCTGGAAG CCTGCGTGGA TGCGGCCCTG CTGGCCACCC AGATGGACCG CGTGAACGCC
    ATCCCCTTCA CCTACGAGCA GCTGGACGTC CTAAAGCATA AACTGGATGA GCTCTACCCA
    CAAGGTTACC CCGAGTCTGT GATCCAGCAC CTGGGCTACC TCTTCCTCAA GATGAGCCCT
    GAGGACATTC GCAAGTGGAA TGTGACGTCC CTGGAGACCC TGAAGGCTTT GCTTGAAGTC
    AACAAAGGGC ACGAAATGAG TCCTCAGGTG GCCACCCTGA TCGACCGCTT TGTGAAGGGA
    AGGGGCCAGC TAGACAAAGA CACCCTAGAC ACCCTGACCG CCTTCTACCC TGGGTACCTG
    TGCTCCCTCA GCCCCGAGGA GCTGAGCTCC GTGCCCCCCA GCAGCATCTG GGCGGTCAGG
    CCCCAGGACC TGGACACGTG TGACCCAAGG CAGCTGGACG TCCTCTATCC CAAGGCCCGC
    CTTGCTTTCC AGAACATGAA CGGGTCCGAA TACTTCGTGA AGATCCAGTC CTTCCTGGGT
    GGGGCCCCCA CGGAGGATTT GAAGGCGCTC AGTCAGCAGA ATGTGAGCAT GGACTTGGCC
    ACGTTCATGA AGCTGCGGAC GGATGCGGTG CTGCCGTTGA CTGTGGCTGA GGTGCAGAAA
    CTTCTGGGAC CCCACGTGGA GGGCCTGAAG GCGGAGGAGC GGCACCGCCC GGTGCGGGAC
    TGGATCCTAC GGCAGCGGCA GGACGACCTG GACACGCTGG GGCTGGGGCT ACAGGGCGGC
    ATCCCCAACG GCTACCTGGT CCTAGACCTC AGCATGCAAG AGGCCCTCTC GGGGACGCCC
    TGCCTCCTAG GACCTGGACC TGTTCTCACC GTCCTGGCAC TGCTCCTAGC CTCCACCCTG
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005255091.1
    CDS418..2283
    Translation

    Target ORF information:

    RefSeq Version XM_005255034.4
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005255034.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG CTGCGCCCCT GGACGGAGTC CTGGCCAACC CACCTAACAT TTCCAGCCTC
    TCCCCTCGCC AACTCCTTGG CTTCCCGTGT GCGGAGGTGT CCGGCCTGAG CACGGAGCGT
    GTCCGGGAGC TGGCTGTGGC CTTGGCACAG AAGAATGTCA AGCTCTCAAC AGAGCAGCTG
    CGCTGTCTGG CTCACCGGCT CTCTGAGCCC CCCGAGGACC TGGACGCCCT CCCATTGGAC
    CTGCTGCTAT TCCTCAACCC AGATGCGTTC TCGGGGCCCC AGGCCTGCAC CCGTTTCTTC
    TCCCGCATCA CGAAGGCCAA TGTGGACCTG CTCCCGAGGG GGGCTCCCGA GCGACAGCGG
    CTGCTGCCTG CGGCTCTGGC CTGCTGGGGT GTGCGGGGGT CTCTGCTGAG CGAGGCTGAT
    GTGCGGGCTC TGGGAGGCCT GGCTTGCGAC CTGCCTGGGC GCTTTGTGGC CGAGTCGGCC
    GAAGTGCTGC TACCCCGGCT GGTGAGCTGC CCGGGACCCC TGGACCAGGA CCAGCAGGAG
    GCAGCCAGGG CGGCTCTGCA GGGCGGGGGA CCCCCCTACG GCCCCCCGTC GACATGGTCT
    GTCTCCACGA TGGACGCTCT GCGGGGCCTG CTGCCCGTGC TGGGCCAGCC CATCATCCGC
    AGCATCCCGC AGGGCATCGT GGCCGCGTGG CGGCAACGCT CCTCTCGGGA CCCATCCTGG
    CGGCAGCCTG AACGGACCAT CCTCCGGCCG CGGTTCCGGC GGGAAGTGGA GAAGACAGCC
    TGTCCTTCAG GCAAGAAGGC CCGCGAGATA GACGAGAGCC TCATCTTCTA CAAGAAGTGG
    GAGCTGGAAG CCTGCGTGGA TGCGGCCCTG CTGGCCACCC AGATGGACCG CGTGAACGCC
    ATCCCCTTCA CCTACGAGCA GCTGGACGTC CTAAAGCATA AACTGGATGA GCTCTACCCA
    CAAGGTTACC CCGAGTCTGT GATCCAGCAC CTGGGCTACC TCTTCCTCAA GATGAGCCCT
    GAGGACATTC GCAAGTGGAA TGTGACGTCC CTGGAGACCC TGAAGGCTTT GCTTGAAGTC
    AACAAAGGGC ACGAAATGAG TCCTCAGGTG GCCACCCTGA TCGACCGCTT TGTGAAGGGA
    AGGGGCCAGC TAGACAAAGA CACCCTAGAC ACCCTGACCG CCTTCTACCC TGGGTACCTG
    TGCTCCCTCA GCCCCGAGGA GCTGAGCTCC GTGCCCCCCA GCAGCATCTG GGCGGTCAGG
    CCCCAGGACC TGGACACGTG TGACCCAAGG CAGCTGGACG TCCTCTATCC CAAGGCCCGC
    CTTGCTTTCC AGAACATGAA CGGGTCCGAA TACTTCGTGA AGATCCAGTC CTTCCTGGGT
    GGGGCCCCCA CGGAGGATTT GAAGGCGCTC AGTCAGCAGA ATGTGAGCAT GGACTTGGCC
    ACGTTCATGA AGCTGCGGAC GGATGCGGTG CTGCCGTTGA CTGTGGCTGA GGTGCAGAAA
    CTTCTGGGAC CCCACGTGGA GGGCCTGAAG GCGGAGGAGC GGCACCGCCC GGTGCGGGAC
    TGGATCCTAC GGCAGCGGCA GGACGACCTG GACACGCTGG GGCTGGGGCT ACAGGGCGGC
    ATCCCCAACG GCTACCTGGT CCTAGACCTC AGCATGCAAG AGGCCCTCTC GGGGACGCCC
    TGCCTCCTAG GACCTGGACC TGTTCTCACC GTCCTGGCAC TGCTCCTAGC CTCCACCCTG
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63786
    Clone ID Related Accession (Same CDS sequence) XM_017022858.1
    Accession Version XM_017022858.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mesothelin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG CTGCGCCCCT GGACGGAGTC CTGGCCAACC CACCTAACAT TTCCAGCCTC
    TCCCCTCGCC AACTCCTTGG CTTCCCGTGT GCGGAGGTGT CCGGCCTGAG CACGGAGCGT
    GTCCGGGAGC TGGCTGTGGC CTTGGCACAG AAGAATGTCA AGCTCTCAAC AGAGCAGCTG
    CGCTGTCTGG CTCACCGGCT CTCTGAGCCC CCCGAGGACC TGGACGCCCT CCCATTGGAC
    CTGCTGCTAT TCCTCAACCC AGATGCGTTC TCGGGGCCCC AGGCCTGCAC CCGTTTCTTC
    TCCCGCATCA CGAAGGCCAA TGTGGACCTG CTCCCGAGGG GGGCTCCCGA GCGACAGCGG
    CTGCTGCCTG CGGCTCTGGC CTGCTGGGGT GTGCGGGGGT CTCTGCTGAG CGAGGCTGAT
    GTGCGGGCTC TGGGAGGCCT GGCTTGCGAC CTGCCTGGGC GCTTTGTGGC CGAGTCGGCC
    GAAGTGCTGC TACCCCGGCT GGTGAGCTGC CCGGGACCCC TGGACCAGGA CCAGCAGGAG
    GCAGCCAGGG CGGCTCTGCA GGGCGGGGGA CCCCCCTACG GCCCCCCGTC GACATGGTCT
    GTCTCCACGA TGGACGCTCT GCGGGGCCTG CTGCCCGTGC TGGGCCAGCC CATCATCCGC
    AGCATCCCGC AGGGCATCGT GGCCGCGTGG CGGCAACGCT CCTCTCGGGA CCCATCCTGG
    CGGCAGCCTG AACGGACCAT CCTCCGGCCG CGGTTCCGGC GGGAAGTGGA GAAGACAGCC
    TGTCCTTCAG GCAAGAAGGC CCGCGAGATA GACGAGAGCC TCATCTTCTA CAAGAAGTGG
    GAGCTGGAAG CCTGCGTGGA TGCGGCCCTG CTGGCCACCC AGATGGACCG CGTGAACGCC
    ATCCCCTTCA CCTACGAGCA GCTGGACGTC CTAAAGCATA AACTGGATGA GCTCTACCCA
    CAAGGTTACC CCGAGTCTGT GATCCAGCAC CTGGGCTACC TCTTCCTCAA GATGAGCCCT
    GAGGACATTC GCAAGTGGAA TGTGACGTCC CTGGAGACCC TGAAGGCTTT GCTTGAAGTC
    AACAAAGGGC ACGAAATGAG TCCTCAGGCT CCTCGGCGGC CCCTCCCACA GGTGGCCACC
    CTGATCGACC GCTTTGTGAA GGGAAGGGGC CAGCTAGACA AAGACACCCT AGACACCCTG
    ACCGCCTTCT ACCCTGGGTA CCTGTGCTCC CTCAGCCCCG AGGAGCTGAG CTCCGTGCCC
    CCCAGCAGCA TCTGGGCGGT CAGGCCCCAG GACCTGGACA CGTGTGACCC AAGGCAGCTG
    GACGTCCTCT ATCCCAAGGC CCGCCTTGCT TTCCAGAACA TGAACGGGTC CGAATACTTC
    GTGAAGATCC AGTCCTTCCT GGGTGGGGCC CCCACGGAGG ATTTGAAGGC GCTCAGTCAG
    CAGAATGTGA GCATGGACTT GGCCACGTTC ATGAAGCTGC GGACGGATGC GGTGCTGCCG
    TTGACTGTGG CTGAGGTGCA GAAACTTCTG GGACCCCACG TGGAGGGCCT GAAGGCGGAG
    GAGCGGCACC GCCCGGTGCG GGACTGGATC CTACGGCAGC GGCAGGACGA CCTGGACACG
    CTGGGGCTGG GGCTACAGGG CGGCATCCCC AACGGCTACC TGGTCCTAGA CCTCAGCATG
    CAAGAGGCCC TCTCGGGGAC GCCCTGCCTC CTAGGACCTG GACCTGTTCT CACCGTCCTG
    GCACTGCTCC TAGCCTCCAC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878347.1
    CDS182..2071
    Translation

    Target ORF information:

    RefSeq Version XM_017022858.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022858.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCTTGC CAACGGCTCG ACCCCTGTTG GGGTCCTGTG GGACCCCCGC CCTCGGCAGC 
    CTCCTGTTCC TGCTCTTCAG CCTCGGATGG GTGCAGCCCT CGAGGACCCT GGCTGGAGAG
    ACAGGGCAGG CTGCGCCCCT GGACGGAGTC CTGGCCAACC CACCTAACAT TTCCAGCCTC
    TCCCCTCGCC AACTCCTTGG CTTCCCGTGT GCGGAGGTGT CCGGCCTGAG CACGGAGCGT
    GTCCGGGAGC TGGCTGTGGC CTTGGCACAG AAGAATGTCA AGCTCTCAAC AGAGCAGCTG
    CGCTGTCTGG CTCACCGGCT CTCTGAGCCC CCCGAGGACC TGGACGCCCT CCCATTGGAC
    CTGCTGCTAT TCCTCAACCC AGATGCGTTC TCGGGGCCCC AGGCCTGCAC CCGTTTCTTC
    TCCCGCATCA CGAAGGCCAA TGTGGACCTG CTCCCGAGGG GGGCTCCCGA GCGACAGCGG
    CTGCTGCCTG CGGCTCTGGC CTGCTGGGGT GTGCGGGGGT CTCTGCTGAG CGAGGCTGAT
    GTGCGGGCTC TGGGAGGCCT GGCTTGCGAC CTGCCTGGGC GCTTTGTGGC CGAGTCGGCC
    GAAGTGCTGC TACCCCGGCT GGTGAGCTGC CCGGGACCCC TGGACCAGGA CCAGCAGGAG
    GCAGCCAGGG CGGCTCTGCA GGGCGGGGGA CCCCCCTACG GCCCCCCGTC GACATGGTCT
    GTCTCCACGA TGGACGCTCT GCGGGGCCTG CTGCCCGTGC TGGGCCAGCC CATCATCCGC
    AGCATCCCGC AGGGCATCGT GGCCGCGTGG CGGCAACGCT CCTCTCGGGA CCCATCCTGG
    CGGCAGCCTG AACGGACCAT CCTCCGGCCG CGGTTCCGGC GGGAAGTGGA GAAGACAGCC
    TGTCCTTCAG GCAAGAAGGC CCGCGAGATA GACGAGAGCC TCATCTTCTA CAAGAAGTGG
    GAGCTGGAAG CCTGCGTGGA TGCGGCCCTG CTGGCCACCC AGATGGACCG CGTGAACGCC
    ATCCCCTTCA CCTACGAGCA GCTGGACGTC CTAAAGCATA AACTGGATGA GCTCTACCCA
    CAAGGTTACC CCGAGTCTGT GATCCAGCAC CTGGGCTACC TCTTCCTCAA GATGAGCCCT
    GAGGACATTC GCAAGTGGAA TGTGACGTCC CTGGAGACCC TGAAGGCTTT GCTTGAAGTC
    AACAAAGGGC ACGAAATGAG TCCTCAGGCT CCTCGGCGGC CCCTCCCACA GGTGGCCACC
    CTGATCGACC GCTTTGTGAA GGGAAGGGGC CAGCTAGACA AAGACACCCT AGACACCCTG
    ACCGCCTTCT ACCCTGGGTA CCTGTGCTCC CTCAGCCCCG AGGAGCTGAG CTCCGTGCCC
    CCCAGCAGCA TCTGGGCGGT CAGGCCCCAG GACCTGGACA CGTGTGACCC AAGGCAGCTG
    GACGTCCTCT ATCCCAAGGC CCGCCTTGCT TTCCAGAACA TGAACGGGTC CGAATACTTC
    GTGAAGATCC AGTCCTTCCT GGGTGGGGCC CCCACGGAGG ATTTGAAGGC GCTCAGTCAG
    CAGAATGTGA GCATGGACTT GGCCACGTTC ATGAAGCTGC GGACGGATGC GGTGCTGCCG
    TTGACTGTGG CTGAGGTGCA GAAACTTCTG GGACCCCACG TGGAGGGCCT GAAGGCGGAG
    GAGCGGCACC GCCCGGTGCG GGACTGGATC CTACGGCAGC GGCAGGACGA CCTGGACACG
    CTGGGGCTGG GGCTACAGGG CGGCATCCCC AACGGCTACC TGGTCCTAGA CCTCAGCATG
    CAAGAGGCCC TCTCGGGGAC GCCCTGCCTC CTAGGACCTG GACCTGTTCT CACCGTCCTG
    GCACTGCTCC TAGCCTCCAC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85806
    Clone ID Related Accession (Same CDS sequence) XM_017022853.1
    Accession Version XM_017022853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mesothelin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGGCCT CCCTGAAAGG GACACAGACC ATGGCCTTGC CAACGGCTCG ACCCCTGTTG 
    GGGTCCTGTG GGACCCCCGC CCTCGGCAGC CTCCTGTTCC TGCTCTTCAG CCTCGGATGG
    GTGCAGCCCT CGAGGACCCT GGCTGGAGAG ACAGGGCAGG AGGCTGCGCC CCTGGACGGA
    GTCCTGGCCA ACCCACCTAA CATTTCCAGC CTCTCCCCTC GCCAACTCCT TGGCTTCCCG
    TGTGCGGAGG TGTCCGGCCT GAGCACGGAG CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA
    CAGAAGAATG TCAAGCTCTC AACAGAGCAG CTGCGCTGTC TGGCTCACCG GCTCTCTGAG
    CCCCCCGAGG ACCTGGACGC CCTCCCATTG GACCTGCTGC TATTCCTCAA CCCAGATGCG
    TTCTCGGGGC CCCAGGCCTG CACCCGTTTC TTCTCCCGCA TCACGAAGGC CAATGTGGAC
    CTGCTCCCGA GGGGGGCTCC CGAGCGACAG CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG
    GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT GATGTGCGGG CTCTGGGAGG CCTGGCTTGC
    GACCTGCCTG GGCGCTTTGT GGCCGAGTCG GCCGAAGTGC TGCTACCCCG GCTGGTGAGC
    TGCCCGGGAC CCCTGGACCA GGACCAGCAG GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG
    GGACCCCCCT ACGGCCCCCC GTCGACATGG TCTGTCTCCA CGATGGACGC TCTGCGGGGC
    CTGCTGCCCG TGCTGGGCCA GCCCATCATC CGCAGCATCC CGCAGGGCAT CGTGGCCGCG
    TGGCGGCAAC GCTCCTCTCG GGACCCATCC TGGCGGCAGC CTGAACGGAC CATCCTCCGG
    CCGCGGTTCC GGCGGGAAGT GGAGAAGACA GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG
    ATAGACGAGA GCCTCATCTT CTACAAGAAG TGGGAGCTGG AAGCCTGCGT GGATGCGGCC
    CTGCTGGCCA CCCAGATGGA CCGCGTGAAC GCCATCCCCT TCACCTACGA GCAGCTGGAC
    GTCCTAAAGC ATAAACTGGA TGAGCTCTAC CCACAAGGTT ACCCCGAGTC TGTGATCCAG
    CACCTGGGCT ACCTCTTCCT CAAGATGAGC CCTGAGGACA TTCGCAAGTG GAATGTGACG
    TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA GTCAACAAAG GGCACGAAAT GAGTCCTCAG
    GCTCCTCGGC GGCCCCTCCC ACAGGTGGCC ACCCTGATCG ACCGCTTTGT GAAGGGAAGG
    GGCCAGCTAG ACAAAGACAC CCTAGACACC CTGACCGCCT TCTACCCTGG GTACCTGTGC
    TCCCTCAGCC CCGAGGAGCT GAGCTCCGTG CCCCCCAGCA GCATCTGGGC GGTCAGGCCC
    CAGGACCTGG ACACGTGTGA CCCAAGGCAG CTGGACGTCC TCTATCCCAA GGCCCGCCTT
    GCTTTCCAGA ACATGAACGG GTCCGAATAC TTCGTGAAGA TCCAGTCCTT CCTGGGTGGG
    GCCCCCACGG AGGATTTGAA GGCGCTCAGT CAGCAGAATG TGAGCATGGA CTTGGCCACG
    TTCATGAAGC TGCGGACGGA TGCGGTGCTG CCGTTGACTG TGGCTGAGGT GCAGAAACTT
    CTGGGACCCC ACGTGGAGGG CCTGAAGGCG GAGGAGCGGC ACCGCCCGGT GCGGGACTGG
    ATCCTACGGC AGCGGCAGGA CGACCTGGAC ACGCTGGGGC TGGGGCTACA GGGCGGCATC
    CCCAACGGCT ACCTGGTCCT AGACCTCAGC ATGCAAGAGG CCCTCTCGGG GACGCCCTGC
    CTCCTAGGAC CTGGACCTGT TCTCACCGTC CTGGCACTGC TCCTAGCCTC CACCCTGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878342.1
    CDS299..2221
    Translation

    Target ORF information:

    RefSeq Version XM_017022853.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGGCCT CCCTGAAAGG GACACAGACC ATGGCCTTGC CAACGGCTCG ACCCCTGTTG 
    GGGTCCTGTG GGACCCCCGC CCTCGGCAGC CTCCTGTTCC TGCTCTTCAG CCTCGGATGG
    GTGCAGCCCT CGAGGACCCT GGCTGGAGAG ACAGGGCAGG AGGCTGCGCC CCTGGACGGA
    GTCCTGGCCA ACCCACCTAA CATTTCCAGC CTCTCCCCTC GCCAACTCCT TGGCTTCCCG
    TGTGCGGAGG TGTCCGGCCT GAGCACGGAG CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA
    CAGAAGAATG TCAAGCTCTC AACAGAGCAG CTGCGCTGTC TGGCTCACCG GCTCTCTGAG
    CCCCCCGAGG ACCTGGACGC CCTCCCATTG GACCTGCTGC TATTCCTCAA CCCAGATGCG
    TTCTCGGGGC CCCAGGCCTG CACCCGTTTC TTCTCCCGCA TCACGAAGGC CAATGTGGAC
    CTGCTCCCGA GGGGGGCTCC CGAGCGACAG CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG
    GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT GATGTGCGGG CTCTGGGAGG CCTGGCTTGC
    GACCTGCCTG GGCGCTTTGT GGCCGAGTCG GCCGAAGTGC TGCTACCCCG GCTGGTGAGC
    TGCCCGGGAC CCCTGGACCA GGACCAGCAG GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG
    GGACCCCCCT ACGGCCCCCC GTCGACATGG TCTGTCTCCA CGATGGACGC TCTGCGGGGC
    CTGCTGCCCG TGCTGGGCCA GCCCATCATC CGCAGCATCC CGCAGGGCAT CGTGGCCGCG
    TGGCGGCAAC GCTCCTCTCG GGACCCATCC TGGCGGCAGC CTGAACGGAC CATCCTCCGG
    CCGCGGTTCC GGCGGGAAGT GGAGAAGACA GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG
    ATAGACGAGA GCCTCATCTT CTACAAGAAG TGGGAGCTGG AAGCCTGCGT GGATGCGGCC
    CTGCTGGCCA CCCAGATGGA CCGCGTGAAC GCCATCCCCT TCACCTACGA GCAGCTGGAC
    GTCCTAAAGC ATAAACTGGA TGAGCTCTAC CCACAAGGTT ACCCCGAGTC TGTGATCCAG
    CACCTGGGCT ACCTCTTCCT CAAGATGAGC CCTGAGGACA TTCGCAAGTG GAATGTGACG
    TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA GTCAACAAAG GGCACGAAAT GAGTCCTCAG
    GCTCCTCGGC GGCCCCTCCC ACAGGTGGCC ACCCTGATCG ACCGCTTTGT GAAGGGAAGG
    GGCCAGCTAG ACAAAGACAC CCTAGACACC CTGACCGCCT TCTACCCTGG GTACCTGTGC
    TCCCTCAGCC CCGAGGAGCT GAGCTCCGTG CCCCCCAGCA GCATCTGGGC GGTCAGGCCC
    CAGGACCTGG ACACGTGTGA CCCAAGGCAG CTGGACGTCC TCTATCCCAA GGCCCGCCTT
    GCTTTCCAGA ACATGAACGG GTCCGAATAC TTCGTGAAGA TCCAGTCCTT CCTGGGTGGG
    GCCCCCACGG AGGATTTGAA GGCGCTCAGT CAGCAGAATG TGAGCATGGA CTTGGCCACG
    TTCATGAAGC TGCGGACGGA TGCGGTGCTG CCGTTGACTG TGGCTGAGGT GCAGAAACTT
    CTGGGACCCC ACGTGGAGGG CCTGAAGGCG GAGGAGCGGC ACCGCCCGGT GCGGGACTGG
    ATCCTACGGC AGCGGCAGGA CGACCTGGAC ACGCTGGGGC TGGGGCTACA GGGCGGCATC
    CCCAACGGCT ACCTGGTCCT AGACCTCAGC ATGCAAGAGG CCCTCTCGGG GACGCCCTGC
    CTCCTAGGAC CTGGACCTGT TCTCACCGTC CTGGCACTGC TCCTAGCCTC CACCCTGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85807
    Clone ID Related Accession (Same CDS sequence) XM_017022854.1
    Accession Version XM_017022854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mesothelin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTGGCCT CCCTGAAAGG GACACAGACC ATGGCCTTGC CAACGGCTCG ACCCCTGTTG 
    GGGTCCTGTG GGACCCCCGC CCTCGGCAGC CTCCTGTTCC TGCTCTTCAG CCTCGGATGG
    GTGCAGCCCT CGAGGACCCT GGCTGGAGAG ACAGGGCAGG CTGCGCCCCT GGACGGAGTC
    CTGGCCAACC CACCTAACAT TTCCAGCCTC TCCCCTCGCC AACTCCTTGG CTTCCCGTGT
    GCGGAGGTGT CCGGCCTGAG CACGGAGCGT GTCCGGGAGC TGGCTGTGGC CTTGGCACAG
    AAGAATGTCA AGCTCTCAAC AGAGCAGCTG CGCTGTCTGG CTCACCGGCT CTCTGAGCCC
    CCCGAGGACC TGGACGCCCT CCCATTGGAC CTGCTGCTAT TCCTCAACCC AGATGCGTTC
    TCGGGGCCCC AGGCCTGCAC CCGTTTCTTC TCCCGCATCA CGAAGGCCAA TGTGGACCTG
    CTCCCGAGGG GGGCTCCCGA GCGACAGCGG CTGCTGCCTG CGGCTCTGGC CTGCTGGGGT
    GTGCGGGGGT CTCTGCTGAG CGAGGCTGAT GTGCGGGCTC TGGGAGGCCT GGCTTGCGAC
    CTGCCTGGGC GCTTTGTGGC CGAGTCGGCC GAAGTGCTGC TACCCCGGCT GGTGAGCTGC
    CCGGGACCCC TGGACCAGGA CCAGCAGGAG GCAGCCAGGG CGGCTCTGCA GGGCGGGGGA
    CCCCCCTACG GCCCCCCGTC GACATGGTCT GTCTCCACGA TGGACGCTCT GCGGGGCCTG
    CTGCCCGTGC TGGGCCAGCC CATCATCCGC AGCATCCCGC AGGGCATCGT GGCCGCGTGG
    CGGCAACGCT CCTCTCGGGA CCCATCCTGG CGGCAGCCTG AACGGACCAT CCTCCGGCCG
    CGGTTCCGGC GGGAAGTGGA GAAGACAGCC TGTCCTTCAG GCAAGAAGGC CCGCGAGATA
    GACGAGAGCC TCATCTTCTA CAAGAAGTGG GAGCTGGAAG CCTGCGTGGA TGCGGCCCTG
    CTGGCCACCC AGATGGACCG CGTGAACGCC ATCCCCTTCA CCTACGAGCA GCTGGACGTC
    CTAAAGCATA AACTGGATGA GCTCTACCCA CAAGGTTACC CCGAGTCTGT GATCCAGCAC
    CTGGGCTACC TCTTCCTCAA GATGAGCCCT GAGGACATTC GCAAGTGGAA TGTGACGTCC
    CTGGAGACCC TGAAGGCTTT GCTTGAAGTC AACAAAGGGC ACGAAATGAG TCCTCAGGCT
    CCTCGGCGGC CCCTCCCACA GGTGGCCACC CTGATCGACC GCTTTGTGAA GGGAAGGGGC
    CAGCTAGACA AAGACACCCT AGACACCCTG ACCGCCTTCT ACCCTGGGTA CCTGTGCTCC
    CTCAGCCCCG AGGAGCTGAG CTCCGTGCCC CCCAGCAGCA TCTGGGCGGT CAGGCCCCAG
    GACCTGGACA CGTGTGACCC AAGGCAGCTG GACGTCCTCT ATCCCAAGGC CCGCCTTGCT
    TTCCAGAACA TGAACGGGTC CGAATACTTC GTGAAGATCC AGTCCTTCCT GGGTGGGGCC
    CCCACGGAGG ATTTGAAGGC GCTCAGTCAG CAGAATGTGA GCATGGACTT GGCCACGTTC
    ATGAAGCTGC GGACGGATGC GGTGCTGCCG TTGACTGTGG CTGAGGTGCA GAAACTTCTG
    GGACCCCACG TGGAGGGCCT GAAGGCGGAG GAGCGGCACC GCCCGGTGCG GGACTGGATC
    CTACGGCAGC GGCAGGACGA CCTGGACACG CTGGGGCTGG GGCTACAGGG CGGCATCCCC
    AACGGCTACC TGGTCCTAGA CCTCAGCATG CAAGAGGCCC TCTCGGGGAC GCCCTGCCTC
    CTAGGACCTG GACCTGTTCT CACCGTCCTG GCACTGCTCC TAGCCTCCAC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878343.1
    CDS313..2232
    Translation

    Target ORF information:

    RefSeq Version XM_017022854.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTGGCCT CCCTGAAAGG GACACAGACC ATGGCCTTGC CAACGGCTCG ACCCCTGTTG 
    GGGTCCTGTG GGACCCCCGC CCTCGGCAGC CTCCTGTTCC TGCTCTTCAG CCTCGGATGG
    GTGCAGCCCT CGAGGACCCT GGCTGGAGAG ACAGGGCAGG CTGCGCCCCT GGACGGAGTC
    CTGGCCAACC CACCTAACAT TTCCAGCCTC TCCCCTCGCC AACTCCTTGG CTTCCCGTGT
    GCGGAGGTGT CCGGCCTGAG CACGGAGCGT GTCCGGGAGC TGGCTGTGGC CTTGGCACAG
    AAGAATGTCA AGCTCTCAAC AGAGCAGCTG CGCTGTCTGG CTCACCGGCT CTCTGAGCCC
    CCCGAGGACC TGGACGCCCT CCCATTGGAC CTGCTGCTAT TCCTCAACCC AGATGCGTTC
    TCGGGGCCCC AGGCCTGCAC CCGTTTCTTC TCCCGCATCA CGAAGGCCAA TGTGGACCTG
    CTCCCGAGGG GGGCTCCCGA GCGACAGCGG CTGCTGCCTG CGGCTCTGGC CTGCTGGGGT
    GTGCGGGGGT CTCTGCTGAG CGAGGCTGAT GTGCGGGCTC TGGGAGGCCT GGCTTGCGAC
    CTGCCTGGGC GCTTTGTGGC CGAGTCGGCC GAAGTGCTGC TACCCCGGCT GGTGAGCTGC
    CCGGGACCCC TGGACCAGGA CCAGCAGGAG GCAGCCAGGG CGGCTCTGCA GGGCGGGGGA
    CCCCCCTACG GCCCCCCGTC GACATGGTCT GTCTCCACGA TGGACGCTCT GCGGGGCCTG
    CTGCCCGTGC TGGGCCAGCC CATCATCCGC AGCATCCCGC AGGGCATCGT GGCCGCGTGG
    CGGCAACGCT CCTCTCGGGA CCCATCCTGG CGGCAGCCTG AACGGACCAT CCTCCGGCCG
    CGGTTCCGGC GGGAAGTGGA GAAGACAGCC TGTCCTTCAG GCAAGAAGGC CCGCGAGATA
    GACGAGAGCC TCATCTTCTA CAAGAAGTGG GAGCTGGAAG CCTGCGTGGA TGCGGCCCTG
    CTGGCCACCC AGATGGACCG CGTGAACGCC ATCCCCTTCA CCTACGAGCA GCTGGACGTC
    CTAAAGCATA AACTGGATGA GCTCTACCCA CAAGGTTACC CCGAGTCTGT GATCCAGCAC
    CTGGGCTACC TCTTCCTCAA GATGAGCCCT GAGGACATTC GCAAGTGGAA TGTGACGTCC
    CTGGAGACCC TGAAGGCTTT GCTTGAAGTC AACAAAGGGC ACGAAATGAG TCCTCAGGCT
    CCTCGGCGGC CCCTCCCACA GGTGGCCACC CTGATCGACC GCTTTGTGAA GGGAAGGGGC
    CAGCTAGACA AAGACACCCT AGACACCCTG ACCGCCTTCT ACCCTGGGTA CCTGTGCTCC
    CTCAGCCCCG AGGAGCTGAG CTCCGTGCCC CCCAGCAGCA TCTGGGCGGT CAGGCCCCAG
    GACCTGGACA CGTGTGACCC AAGGCAGCTG GACGTCCTCT ATCCCAAGGC CCGCCTTGCT
    TTCCAGAACA TGAACGGGTC CGAATACTTC GTGAAGATCC AGTCCTTCCT GGGTGGGGCC
    CCCACGGAGG ATTTGAAGGC GCTCAGTCAG CAGAATGTGA GCATGGACTT GGCCACGTTC
    ATGAAGCTGC GGACGGATGC GGTGCTGCCG TTGACTGTGG CTGAGGTGCA GAAACTTCTG
    GGACCCCACG TGGAGGGCCT GAAGGCGGAG GAGCGGCACC GCCCGGTGCG GGACTGGATC
    CTACGGCAGC GGCAGGACGA CCTGGACACG CTGGGGCTGG GGCTACAGGG CGGCATCCCC
    AACGGCTACC TGGTCCTAGA CCTCAGCATG CAAGAGGCCC TCTCGGGGAC GCCCTGCCTC
    CTAGGACCTG GACCTGTTCT CACCGTCCTG GCACTGCTCC TAGCCTCCAC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85808
    Clone ID Related Accession (Same CDS sequence) XM_017022855.1
    Accession Version XM_017022855.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mesothelin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTGGCCT CCCTGAAAGG GACACAGACC ATGGCCTTGC CAACGGCTCG ACCCCTGTTG 
    GGGTCCTGTG GGACCCCCGC CCTCGGCAGC CTCCTGTTCC TGCTCTTCAG CCTCGGATGG
    GTGCAGCCCT CGAGGACCCT GGCTGGAGAG ACAGGGCAGG AGGCTGCGCC CCTGGACGGA
    GTCCTGGCCA ACCCACCTAA CATTTCCAGC CTCTCCCCTC GCCAACTCCT TGGCTTCCCG
    TGTGCGGAGG TGTCCGGCCT GAGCACGGAG CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA
    CAGAAGAATG TCAAGCTCTC AACAGAGCAG CTGCGCTGTC TGGCTCACCG GCTCTCTGAG
    CCCCCCGAGG ACCTGGACGC CCTCCCATTG GACCTGCTGC TATTCCTCAA CCCAGATGCG
    TTCTCGGGGC CCCAGGCCTG CACCCGTTTC TTCTCCCGCA TCACGAAGGC CAATGTGGAC
    CTGCTCCCGA GGGGGGCTCC CGAGCGACAG CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG
    GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT GATGTGCGGG CTCTGGGAGG CCTGGCTTGC
    GACCTGCCTG GGCGCTTTGT GGCCGAGTCG GCCGAAGTGC TGCTACCCCG GCTGGTGAGC
    TGCCCGGGAC CCCTGGACCA GGACCAGCAG GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG
    GGACCCCCCT ACGGCCCCCC GTCGACATGG TCTGTCTCCA CGATGGACGC TCTGCGGGGC
    CTGCTGCCCG TGCTGGGCCA GCCCATCATC CGCAGCATCC CGCAGGGCAT CGTGGCCGCG
    TGGCGGCAAC GCTCCTCTCG GGACCCATCC TGGCGGCAGC CTGAACGGAC CATCCTCCGG
    CCGCGGTTCC GGCGGGAAGT GGAGAAGACA GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG
    ATAGACGAGA GCCTCATCTT CTACAAGAAG TGGGAGCTGG AAGCCTGCGT GGATGCGGCC
    CTGCTGGCCA CCCAGATGGA CCGCGTGAAC GCCATCCCCT TCACCTACGA GCAGCTGGAC
    GTCCTAAAGC ATAAACTGGA TGAGCTCTAC CCACAAGGTT ACCCCGAGTC TGTGATCCAG
    CACCTGGGCT ACCTCTTCCT CAAGATGAGC CCTGAGGACA TTCGCAAGTG GAATGTGACG
    TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA GTCAACAAAG GGCACGAAAT GAGTCCTCAG
    GTGGCCACCC TGATCGACCG CTTTGTGAAG GGAAGGGGCC AGCTAGACAA AGACACCCTA
    GACACCCTGA CCGCCTTCTA CCCTGGGTAC CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC
    TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC AGGCCCCAGG ACCTGGACAC GTGTGACCCA
    AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC CGCCTTGCTT TCCAGAACAT GAACGGGTCC
    GAATACTTCG TGAAGATCCA GTCCTTCCTG GGTGGGGCCC CCACGGAGGA TTTGAAGGCG
    CTCAGTCAGC AGAATGTGAG CATGGACTTG GCCACGTTCA TGAAGCTGCG GACGGATGCG
    GTGCTGCCGT TGACTGTGGC TGAGGTGCAG AAACTTCTGG GACCCCACGT GGAGGGCCTG
    AAGGCGGAGG AGCGGCACCG CCCGGTGCGG GACTGGATCC TACGGCAGCG GCAGGACGAC
    CTGGACACGC TGGGGCTGGG GCTACAGGGC GGCATCCCCA ACGGCTACCT GGTCCTAGAC
    CTCAGCATGC AAGAGGCCCT CTCGGGGACG CCCTGCCTCC TAGGACCTGG ACCTGTTCTC
    ACCGTCCTGG CACTGCTCCT AGCCTCCACC CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878344.1
    CDS232..2130
    Translation

    Target ORF information:

    RefSeq Version XM_017022855.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022855.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTGGCCT CCCTGAAAGG GACACAGACC ATGGCCTTGC CAACGGCTCG ACCCCTGTTG 
    GGGTCCTGTG GGACCCCCGC CCTCGGCAGC CTCCTGTTCC TGCTCTTCAG CCTCGGATGG
    GTGCAGCCCT CGAGGACCCT GGCTGGAGAG ACAGGGCAGG AGGCTGCGCC CCTGGACGGA
    GTCCTGGCCA ACCCACCTAA CATTTCCAGC CTCTCCCCTC GCCAACTCCT TGGCTTCCCG
    TGTGCGGAGG TGTCCGGCCT GAGCACGGAG CGTGTCCGGG AGCTGGCTGT GGCCTTGGCA
    CAGAAGAATG TCAAGCTCTC AACAGAGCAG CTGCGCTGTC TGGCTCACCG GCTCTCTGAG
    CCCCCCGAGG ACCTGGACGC CCTCCCATTG GACCTGCTGC TATTCCTCAA CCCAGATGCG
    TTCTCGGGGC CCCAGGCCTG CACCCGTTTC TTCTCCCGCA TCACGAAGGC CAATGTGGAC
    CTGCTCCCGA GGGGGGCTCC CGAGCGACAG CGGCTGCTGC CTGCGGCTCT GGCCTGCTGG
    GGTGTGCGGG GGTCTCTGCT GAGCGAGGCT GATGTGCGGG CTCTGGGAGG CCTGGCTTGC
    GACCTGCCTG GGCGCTTTGT GGCCGAGTCG GCCGAAGTGC TGCTACCCCG GCTGGTGAGC
    TGCCCGGGAC CCCTGGACCA GGACCAGCAG GAGGCAGCCA GGGCGGCTCT GCAGGGCGGG
    GGACCCCCCT ACGGCCCCCC GTCGACATGG TCTGTCTCCA CGATGGACGC TCTGCGGGGC
    CTGCTGCCCG TGCTGGGCCA GCCCATCATC CGCAGCATCC CGCAGGGCAT CGTGGCCGCG
    TGGCGGCAAC GCTCCTCTCG GGACCCATCC TGGCGGCAGC CTGAACGGAC CATCCTCCGG
    CCGCGGTTCC GGCGGGAAGT GGAGAAGACA GCCTGTCCTT CAGGCAAGAA GGCCCGCGAG
    ATAGACGAGA GCCTCATCTT CTACAAGAAG TGGGAGCTGG AAGCCTGCGT GGATGCGGCC
    CTGCTGGCCA CCCAGATGGA CCGCGTGAAC GCCATCCCCT TCACCTACGA GCAGCTGGAC
    GTCCTAAAGC ATAAACTGGA TGAGCTCTAC CCACAAGGTT ACCCCGAGTC TGTGATCCAG
    CACCTGGGCT ACCTCTTCCT CAAGATGAGC CCTGAGGACA TTCGCAAGTG GAATGTGACG
    TCCCTGGAGA CCCTGAAGGC TTTGCTTGAA GTCAACAAAG GGCACGAAAT GAGTCCTCAG
    GTGGCCACCC TGATCGACCG CTTTGTGAAG GGAAGGGGCC AGCTAGACAA AGACACCCTA
    GACACCCTGA CCGCCTTCTA CCCTGGGTAC CTGTGCTCCC TCAGCCCCGA GGAGCTGAGC
    TCCGTGCCCC CCAGCAGCAT CTGGGCGGTC AGGCCCCAGG ACCTGGACAC GTGTGACCCA
    AGGCAGCTGG ACGTCCTCTA TCCCAAGGCC CGCCTTGCTT TCCAGAACAT GAACGGGTCC
    GAATACTTCG TGAAGATCCA GTCCTTCCTG GGTGGGGCCC CCACGGAGGA TTTGAAGGCG
    CTCAGTCAGC AGAATGTGAG CATGGACTTG GCCACGTTCA TGAAGCTGCG GACGGATGCG
    GTGCTGCCGT TGACTGTGGC TGAGGTGCAG AAACTTCTGG GACCCCACGT GGAGGGCCTG
    AAGGCGGAGG AGCGGCACCG CCCGGTGCGG GACTGGATCC TACGGCAGCG GCAGGACGAC
    CTGGACACGC TGGGGCTGGG GCTACAGGGC GGCATCCCCA ACGGCTACCT GGTCCTAGAC
    CTCAGCATGC AAGAGGCCCT CTCGGGGACG CCCTGCCTCC TAGGACCTGG ACCTGTTCTC
    ACCGTCCTGG CACTGCTCCT AGCCTCCACC CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85809
    Clone ID Related Accession (Same CDS sequence) XM_017022856.1
    Accession Version XM_017022856.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mesothelin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTGGCCT CCCTGAAAGG GACACAGACC ATGGCCTTGC CAACGGCTCG ACCCCTGTTG 
    GGGTCCTGTG GGACCCCCGC CCTCGGCAGC CTCCTGTTCC TGCTCTTCAG CCTCGGATGG
    GTGCAGCCCT CGAGGACCCT GGCTGGAGAG ACAGGGCAGG CTGCGCCCCT GGACGGAGTC
    CTGGCCAACC CACCTAACAT TTCCAGCCTC TCCCCTCGCC AACTCCTTGG CTTCCCGTGT
    GCGGAGGTGT CCGGCCTGAG CACGGAGCGT GTCCGGGAGC TGGCTGTGGC CTTGGCACAG
    AAGAATGTCA AGCTCTCAAC AGAGCAGCTG CGCTGTCTGG CTCACCGGCT CTCTGAGCCC
    CCCGAGGACC TGGACGCCCT CCCATTGGAC CTGCTGCTAT TCCTCAACCC AGATGCGTTC
    TCGGGGCCCC AGGCCTGCAC CCGTTTCTTC TCCCGCATCA CGAAGGCCAA TGTGGACCTG
    CTCCCGAGGG GGGCTCCCGA GCGACAGCGG CTGCTGCCTG CGGCTCTGGC CTGCTGGGGT
    GTGCGGGGGT CTCTGCTGAG CGAGGCTGAT GTGCGGGCTC TGGGAGGCCT GGCTTGCGAC
    CTGCCTGGGC GCTTTGTGGC CGAGTCGGCC GAAGTGCTGC TACCCCGGCT GGTGAGCTGC
    CCGGGACCCC TGGACCAGGA CCAGCAGGAG GCAGCCAGGG CGGCTCTGCA GGGCGGGGGA
    CCCCCCTACG GCCCCCCGTC GACATGGTCT GTCTCCACGA TGGACGCTCT GCGGGGCCTG
    CTGCCCGTGC TGGGCCAGCC CATCATCCGC AGCATCCCGC AGGGCATCGT GGCCGCGTGG
    CGGCAACGCT CCTCTCGGGA CCCATCCTGG CGGCAGCCTG AACGGACCAT CCTCCGGCCG
    CGGTTCCGGC GGGAAGTGGA GAAGACAGCC TGTCCTTCAG GCAAGAAGGC CCGCGAGATA
    GACGAGAGCC TCATCTTCTA CAAGAAGTGG GAGCTGGAAG CCTGCGTGGA TGCGGCCCTG
    CTGGCCACCC AGATGGACCG CGTGAACGCC ATCCCCTTCA CCTACGAGCA GCTGGACGTC
    CTAAAGCATA AACTGGATGA GCTCTACCCA CAAGGTTACC CCGAGTCTGT GATCCAGCAC
    CTGGGCTACC TCTTCCTCAA GATGAGCCCT GAGGACATTC GCAAGTGGAA TGTGACGTCC
    CTGGAGACCC TGAAGGCTTT GCTTGAAGTC AACAAAGGGC ACGAAATGAG TCCTCAGGTG
    GCCACCCTGA TCGACCGCTT TGTGAAGGGA AGGGGCCAGC TAGACAAAGA CACCCTAGAC
    ACCCTGACCG CCTTCTACCC TGGGTACCTG TGCTCCCTCA GCCCCGAGGA GCTGAGCTCC
    GTGCCCCCCA GCAGCATCTG GGCGGTCAGG CCCCAGGACC TGGACACGTG TGACCCAAGG
    CAGCTGGACG TCCTCTATCC CAAGGCCCGC CTTGCTTTCC AGAACATGAA CGGGTCCGAA
    TACTTCGTGA AGATCCAGTC CTTCCTGGGT GGGGCCCCCA CGGAGGATTT GAAGGCGCTC
    AGTCAGCAGA ATGTGAGCAT GGACTTGGCC ACGTTCATGA AGCTGCGGAC GGATGCGGTG
    CTGCCGTTGA CTGTGGCTGA GGTGCAGAAA CTTCTGGGAC CCCACGTGGA GGGCCTGAAG
    GCGGAGGAGC GGCACCGCCC GGTGCGGGAC TGGATCCTAC GGCAGCGGCA GGACGACCTG
    GACACGCTGG GGCTGGGGCT ACAGGGCGGC ATCCCCAACG GCTACCTGGT CCTAGACCTC
    AGCATGCAAG AGGCCCTCTC GGGGACGCCC TGCCTCCTAG GACCTGGACC TGTTCTCACC
    GTCCTGGCAC TGCTCCTAGC CTCCACCCTG GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878345.1
    CDS236..2131
    Translation

    Target ORF information:

    RefSeq Version XM_017022856.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mesothelin (MSLN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022856.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGTGGCCT CCCTGAAAGG GACACAGACC ATGGCCTTGC CAACGGCTCG ACCCCTGTTG 
    GGGTCCTGTG GGACCCCCGC CCTCGGCAGC CTCCTGTTCC TGCTCTTCAG CCTCGGATGG
    GTGCAGCCCT CGAGGACCCT GGCTGGAGAG ACAGGGCAGG CTGCGCCCCT GGACGGAGTC
    CTGGCCAACC CACCTAACAT TTCCAGCCTC TCCCCTCGCC AACTCCTTGG CTTCCCGTGT
    GCGGAGGTGT CCGGCCTGAG CACGGAGCGT GTCCGGGAGC TGGCTGTGGC CTTGGCACAG
    AAGAATGTCA AGCTCTCAAC AGAGCAGCTG CGCTGTCTGG CTCACCGGCT CTCTGAGCCC
    CCCGAGGACC TGGACGCCCT CCCATTGGAC CTGCTGCTAT TCCTCAACCC AGATGCGTTC
    TCGGGGCCCC AGGCCTGCAC CCGTTTCTTC TCCCGCATCA CGAAGGCCAA TGTGGACCTG
    CTCCCGAGGG GGGCTCCCGA GCGACAGCGG CTGCTGCCTG CGGCTCTGGC CTGCTGGGGT
    GTGCGGGGGT CTCTGCTGAG CGAGGCTGAT GTGCGGGCTC TGGGAGGCCT GGCTTGCGAC
    CTGCCTGGGC GCTTTGTGGC CGAGTCGGCC GAAGTGCTGC TACCCCGGCT GGTGAGCTGC
    CCGGGACCCC TGGACCAGGA CCAGCAGGAG GCAGCCAGGG CGGCTCTGCA GGGCGGGGGA
    CCCCCCTACG GCCCCCCGTC GACATGGTCT GTCTCCACGA TGGACGCTCT GCGGGGCCTG
    CTGCCCGTGC TGGGCCAGCC CATCATCCGC AGCATCCCGC AGGGCATCGT GGCCGCGTGG
    CGGCAACGCT CCTCTCGGGA CCCATCCTGG CGGCAGCCTG AACGGACCAT CCTCCGGCCG
    CGGTTCCGGC GGGAAGTGGA GAAGACAGCC TGTCCTTCAG GCAAGAAGGC CCGCGAGATA
    GACGAGAGCC TCATCTTCTA CAAGAAGTGG GAGCTGGAAG CCTGCGTGGA TGCGGCCCTG
    CTGGCCACCC AGATGGACCG CGTGAACGCC ATCCCCTTCA CCTACGAGCA GCTGGACGTC
    CTAAAGCATA AACTGGATGA GCTCTACCCA CAAGGTTACC CCGAGTCTGT GATCCAGCAC
    CTGGGCTACC TCTTCCTCAA GATGAGCCCT GAGGACATTC GCAAGTGGAA TGTGACGTCC
    CTGGAGACCC TGAAGGCTTT GCTTGAAGTC AACAAAGGGC ACGAAATGAG TCCTCAGGTG
    GCCACCCTGA TCGACCGCTT TGTGAAGGGA AGGGGCCAGC TAGACAAAGA CACCCTAGAC
    ACCCTGACCG CCTTCTACCC TGGGTACCTG TGCTCCCTCA GCCCCGAGGA GCTGAGCTCC
    GTGCCCCCCA GCAGCATCTG GGCGGTCAGG CCCCAGGACC TGGACACGTG TGACCCAAGG
    CAGCTGGACG TCCTCTATCC CAAGGCCCGC CTTGCTTTCC AGAACATGAA CGGGTCCGAA
    TACTTCGTGA AGATCCAGTC CTTCCTGGGT GGGGCCCCCA CGGAGGATTT GAAGGCGCTC
    AGTCAGCAGA ATGTGAGCAT GGACTTGGCC ACGTTCATGA AGCTGCGGAC GGATGCGGTG
    CTGCCGTTGA CTGTGGCTGA GGTGCAGAAA CTTCTGGGAC CCCACGTGGA GGGCCTGAAG
    GCGGAGGAGC GGCACCGCCC GGTGCGGGAC TGGATCCTAC GGCAGCGGCA GGACGACCTG
    GACACGCTGG GGCTGGGGCT ACAGGGCGGC ATCCCCAACG GCTACCTGGT CCTAGACCTC
    AGCATGCAAG AGGCCCTCTC GGGGACGCCC TGCCTCCTAG GACCTGGACC TGTTCTCACC
    GTCCTGGCAC TGCTCCTAGC CTCCACCCTG GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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