MED12L gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_006713487.3	XP_006713550.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
XM_011512399.3	XP_011510701.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X10
XM_017005678.1	XP_016861167.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
XM_017005681.1	XP_016861170.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X9
XM_017005677.1	XP_016861166.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
XM_011512390.2	XP_011510692.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
XM_017005680.1	XP_016861169.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X7
NM_053002.5	NP_443728.3	mediator of RNA polymerase II transcription subunit 12-like protein
XM_017005676.1	XP_016861165.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
XM_017005679.1	XP_016861168.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
XM_011512394.2	XP_011510696.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X8
XM_011512390.1	XP_011510692.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
XM_011512399.2	XP_011510701.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu75018	XM_011512399.3 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu75013	XM_011512394.2 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu20944	NM_053002.5 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu20944	XM_011512390.2 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu37689	XM_006713487.3 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu93784	XM_017005676.1 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu93785	XM_017005677.1 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu93786	XM_017005678.1 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu93787	XM_017005679.1 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu93788	XM_017005680.1 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu93789	XM_017005681.1 Latest version!	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu20944	XM_011512390.1	Homo sapiens mediator complex subunit 12-like (MED12L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu75018	XM_011512399.2	Homo sapiens mediator complex subunit 12 like (MED12L), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

MED12L ( XM_011512399.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510701.1 Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu75018
Clone ID Related Accession (Same CDS sequence)	XM_011512399.3 , XM_011512399.2
Accession Version	XM_011512399.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512399.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510701.1
CDS	402..4073
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEEQSIMAHMGIDSGTTNIFDEVDKSDFKTDFGSEFPIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGK

Target ORF information:

RefSeq Version	XM_011512399.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512399.3

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_011512399.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510701.1 Homo sapiens mediator complex subunit 12 like (MED12L), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu75018
Clone ID Related Accession (Same CDS sequence)	XM_011512399.3 , XM_011512399.2
Accession Version	XM_011512399.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510701.1
CDS	402..4073
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEEQSIMAHMGIDSGTTNIFDEVDKSDFKTDFGSEFPIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGK

Target ORF information:

RefSeq Version	XM_011512399.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12 like (MED12L), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512399.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_011512394.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510696.1 Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X8, mRNA.

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CloneID	OHu75013
Clone ID Related Accession (Same CDS sequence)	XM_011512394.2
Accession Version	XM_011512394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAGT GTCAGCTCTT TAAAGAATGA TGACTTCACC 
ATGAGAGGTT TGCGATGTGA TGGGAATGCT GATGATATCT GGACTGCCTC ACAAAATCCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACTGCCAATT TAAGAGAATA CGCTAGATAT 
GTACTGAGGA CTATCTGTCA ACAGGAATGG GTAGGAGAGC ATTGCTTAAA AGAACCTGAA 
AGATTATGTA CAGACAAAGA ACTTATATTG GACCCTGTGC TTTCAAATAT GCAAGCACAG 
AAATTACTGC AGCTTATCTG TTATCCTCAT GGCATTAAAG AATGTACCGA GGGGGACAAT 
CTGCAAAGAC AGCACATTAA GCGTATTCTT CAGAATCTTG AGCAGTGGAC ACTGAGGCAA 
TCCTGGTTAG AACTCCAGCT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCT 
GTGGCCGAAA TGAACAACTT ACTGGACAAT ATTGCAAAGG CAACAATAGA GGTATTCCAG 
CAGTCTGCAG ACCTAAATAA TTCTTCTAAT TCTGGCATGA GCCTCTTCAA CCCAAACAGT 
ATTGGAAGTG CTGATACAAG TAGCACGAGA CAGAATGGAA TAAAGACATT CCTAAGTTCC 
TCCGAACGCA GGGGTGTATG GTTGGTGGCC CCCCTCATCG CCAGGTTGCC AACTTCTGTG 
CAAGGAAGAG TGCTGAAAGC CGCTGGGGAA GAGCTGGAGA AGGGACAGCA CTTGGGTTCT 
TCTTCCAAAA AGGAAAGGGA CAGACAGAAA CAGAAAAGTA TGTCTCTTTT GAGTCAACAA 
CCCTTCCTCT CACTGGTACT TACCTGCCTT AAGGGACAAG ATGAACAAAG GGAAGGCCTC 
CTAACATCTC TCCAGAATCA AGTTAACCAG ATTTTAAGTA ACTGGAGAGA AGAACGATAC 
CAAGATGACA TAAAAGCGCG GCAGATGATG CACGAAGCAT TGCAACTCCG CCTAAATTTG 
GTAGGAGGAA TGTTTGACAC GGTGCAGAGG AGCACCCAGT GGACTACAGA CTGGGCCCTG 
CTACTCCTTC AGATCATTAC TTCAGGAACT GTTGACATGC ACACTAACAA TGAATTATTC 
ACAACAGTTC TTGACATGCT GGGTGTTTTA ATCAATGGAA CGTTAGCCTC TGACCTATCA 
AATGCATCCC CTGGGGGATC TGAAGAGAAC AAGCGTGCAT ACATGAATTT AGTAAAGAAA 
CTGAAAAAAG AGCTAGGAGA CAAGCGATCA GAAAGTATTG ACAAAGTTCG ACAGTTACTA 
CCTTTGCCGA AACAGACATG TGATGTCATC ACTTGTGAAC CTATGGGTTC CTTGATTGAC 
ACAAAAGGAA ACAAAATTGC TGGATTTGAC TCTATAGATA AAAAACAGGG TCTCCAGGTC 
TCTACGAAGC AGAAGGTGTC CCCGTGGGAC TTGTTTGAGG GTCAGAAGAA CCCAGCTCCT 
TTGTCCTGGG CCTGGTTTGG GACAGTCCGA GTGGACCGAA GAGTGATCAA GTACGAGGAG 
CAGCATCACC TCCTGCTGTA TCACACACAC CCCATGCCCA AGCCCCGCAG TTACTACCTC 
CAGCCACTGC CCCTGCCTCC TGAGGAGGAA GAGGAAGAGC CCACATCTCC AGTTTCTCAG 
GAACCAGAAA GGAAGTCCGC TGAGCTGTCA GATCAGGGAA AAACCACAAC AGATGAAGAA 
AAGAAAACAA AAGGAAGGAA GCGCAAGACG AAATCTAGCT CAAGAGTTGA TGAATATCCA 
CAGAGCAACA TATACCGAGT GCCTCCTAAT TACTCGCCTA TCTCCTCCCA AATGATGCAC 
CATCCACAGT CCACCTTGTG GGGTTACAAC CTCGTGGGCC AGCCCCAGCA GCCCGGCTTT 
TTCCTTCAGA ACCAATCTCT TACTCCAGGT GGCTCCAGAT TGGACCCTGC AGGCTCCTTT 
GTCCCAACCA ACACCAAACA AGCTCTGTCA AACATGCTAC AGCGGCGCTC AGGCGCCATG 
ATGCAGCCGC CTTCTCTTCA TGCAATCACA TCGCAGCAGC AGTTGATACA GATGAAGCTT 
CTGCAGCAGC AGCAGCAACA GCGACTTCTC AGGCAAGCCC AGACTCGGCC TTTCCAACAG 
AAGACAGTGA TCCATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510696.1
CDS	402..6242
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEEQSIMAHMGIDSGTTNIFDEVDKSDFKTDFGSEFPIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQKTVIHV

Target ORF information:

RefSeq Version	XM_011512394.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512394.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAGT GTCAGCTCTT TAAAGAATGA TGACTTCACC 
ATGAGAGGTT TGCGATGTGA TGGGAATGCT GATGATATCT GGACTGCCTC ACAAAATCCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACTGCCAATT TAAGAGAATA CGCTAGATAT 
GTACTGAGGA CTATCTGTCA ACAGGAATGG GTAGGAGAGC ATTGCTTAAA AGAACCTGAA 
AGATTATGTA CAGACAAAGA ACTTATATTG GACCCTGTGC TTTCAAATAT GCAAGCACAG 
AAATTACTGC AGCTTATCTG TTATCCTCAT GGCATTAAAG AATGTACCGA GGGGGACAAT 
CTGCAAAGAC AGCACATTAA GCGTATTCTT CAGAATCTTG AGCAGTGGAC ACTGAGGCAA 
TCCTGGTTAG AACTCCAGCT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCT 
GTGGCCGAAA TGAACAACTT ACTGGACAAT ATTGCAAAGG CAACAATAGA GGTATTCCAG 
CAGTCTGCAG ACCTAAATAA TTCTTCTAAT TCTGGCATGA GCCTCTTCAA CCCAAACAGT 
ATTGGAAGTG CTGATACAAG TAGCACGAGA CAGAATGGAA TAAAGACATT CCTAAGTTCC 
TCCGAACGCA GGGGTGTATG GTTGGTGGCC CCCCTCATCG CCAGGTTGCC AACTTCTGTG 
CAAGGAAGAG TGCTGAAAGC CGCTGGGGAA GAGCTGGAGA AGGGACAGCA CTTGGGTTCT 
TCTTCCAAAA AGGAAAGGGA CAGACAGAAA CAGAAAAGTA TGTCTCTTTT GAGTCAACAA 
CCCTTCCTCT CACTGGTACT TACCTGCCTT AAGGGACAAG ATGAACAAAG GGAAGGCCTC 
CTAACATCTC TCCAGAATCA AGTTAACCAG ATTTTAAGTA ACTGGAGAGA AGAACGATAC 
CAAGATGACA TAAAAGCGCG GCAGATGATG CACGAAGCAT TGCAACTCCG CCTAAATTTG 
GTAGGAGGAA TGTTTGACAC GGTGCAGAGG AGCACCCAGT GGACTACAGA CTGGGCCCTG 
CTACTCCTTC AGATCATTAC TTCAGGAACT GTTGACATGC ACACTAACAA TGAATTATTC 
ACAACAGTTC TTGACATGCT GGGTGTTTTA ATCAATGGAA CGTTAGCCTC TGACCTATCA 
AATGCATCCC CTGGGGGATC TGAAGAGAAC AAGCGTGCAT ACATGAATTT AGTAAAGAAA 
CTGAAAAAAG AGCTAGGAGA CAAGCGATCA GAAAGTATTG ACAAAGTTCG ACAGTTACTA 
CCTTTGCCGA AACAGACATG TGATGTCATC ACTTGTGAAC CTATGGGTTC CTTGATTGAC 
ACAAAAGGAA ACAAAATTGC TGGATTTGAC TCTATAGATA AAAAACAGGG TCTCCAGGTC 
TCTACGAAGC AGAAGGTGTC CCCGTGGGAC TTGTTTGAGG GTCAGAAGAA CCCAGCTCCT 
TTGTCCTGGG CCTGGTTTGG GACAGTCCGA GTGGACCGAA GAGTGATCAA GTACGAGGAG 
CAGCATCACC TCCTGCTGTA TCACACACAC CCCATGCCCA AGCCCCGCAG TTACTACCTC 
CAGCCACTGC CCCTGCCTCC TGAGGAGGAA GAGGAAGAGC CCACATCTCC AGTTTCTCAG 
GAACCAGAAA GGAAGTCCGC TGAGCTGTCA GATCAGGGAA AAACCACAAC AGATGAAGAA 
AAGAAAACAA AAGGAAGGAA GCGCAAGACG AAATCTAGCT CAAGAGTTGA TGAATATCCA 
CAGAGCAACA TATACCGAGT GCCTCCTAAT TACTCGCCTA TCTCCTCCCA AATGATGCAC 
CATCCACAGT CCACCTTGTG GGGTTACAAC CTCGTGGGCC AGCCCCAGCA GCCCGGCTTT 
TTCCTTCAGA ACCAATCTCT TACTCCAGGT GGCTCCAGAT TGGACCCTGC AGGCTCCTTT 
GTCCCAACCA ACACCAAACA AGCTCTGTCA AACATGCTAC AGCGGCGCTC AGGCGCCATG 
ATGCAGCCGC CTTCTCTTCA TGCAATCACA TCGCAGCAGC AGTTGATACA GATGAAGCTT 
CTGCAGCAGC AGCAGCAACA GCGACTTCTC AGGCAAGCCC AGACTCGGCC TTTCCAACAG 
AAGACAGTGA TCCATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( NM_053002.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_443728.3 Homo sapiens mediator complex subunit 12L (MED12L), mRNA.

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CloneID	OHu20944
Clone ID Related Accession (Same CDS sequence)	XM_011512390.2 , NM_053002.5 , XM_011512390.1
Accession Version	NM_053002.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK128165.1, AF399708.1, AC078816.16 and AC024886.20. This sequence is a reference standard in the RefSeqGene project. On Aug 22, 2014 this sequence version replaced NM_053002.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF399708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGAT TTTTTCTCCT 
ATGCCTGGAG AATCCTGTGA GAATGCCAAC ACTTCGTTGG GCAGAAGAAT GTCAGTTAAT 
TGTGAGAAGT TGGTGAAGAG GGAAAAGCCA AGGGAATTAA TTTTTCCATC TAATTATGAC 
CTCCTTCGCC ACTTACAGTA TGCAACACAT TTTCCTATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTC TATGGAGTCG GCAAAGAGCG TGATGAAGCA 
AGGCATCAGC TGAAGAAGAT TACCAAAGAT ATCCTGAAAA TTCTAAATAA GAAGAGCACC 
ACAGAGACAG GGGTTGGGGA CGAAGGACAA AAAGCCAGGA AGAACAAACA GGAGACATTT 
CCAACACTGG AGACTGTGTT CACTAAACTC CAGCTCCTTT CATATTTTGA TCAACATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC GTCAGGAACA 
TCCTATCATC TCCCTTTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTGAACATCA ACGGACTAAT TGACTTCGCA ATACAGTTAC TAAATGAACT GAGTGTTGTG 
GAAGCTGAAC TGCTCCTAAA ATCCTCCAGC CTGGCAGGAA GTTATACAAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGCTAT CACAGTTGTC TAATCTTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG GTTGTGTGGT GTGGTCAAGC ATGTCGTAAA CCCCTCAGAA 
TGTTCTTCCC CTGAAAGATG CATTTTAGCC TACCTCTATG ATCTCTATGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAATA ACGTGATGCC TGCAAATTCG AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC AGCCCGCAGC ATCAACTACT CAATGCTGGG CAAGATCCTC 
AGTGACAATG CGGCCAATCG CTACAGCTTT GTCTGCAATA CACTCATGAA TGTATGTATG 
GGCCATCAGG ATGCTGGCAG GATTAACGAC ATAGCCAATT TCTCCTCTGA GCTTACGGCT 
TGCTGCACTG TTCTTAGTTC AGAATGGCTG GGGGTTCTGA AGGCTCTTTG TTGTTCTTCA 
AATCACGTGT GGGGGTTTAA TGATGTACTT TGCACTGTAG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT TTCCCTGGAG 
GACGTCGTGC AGCATGTCGC ACTTCCCTCT CTTCTAGCAG CAGCTTGTGG GGATGCGGAC 
GCCGAGCCTG GGGCGAGAAT GACATGCCGA CTCTTGCTTC ATCTCTTCCG AGCTCCCCAG 
GCCTGCTTCT TACCTCAAGC AACGGGCAAA CCTTTCCCTG GAATAAGATC ATCTTGTGAT 
AGACACCTCT TAGCCGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAG 
AATGATGACT TCACCATGAG AGGTTTGCGA TGTGATGGGA ATGCTGATGA TATCTGGACT 
GCCTCACAAA ATCCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACTGC CAATTTAAGA 
GAATACGCTA GATATGTACT GAGGACTATC TGTCAACAGG AATGGGTAGG AGAGCATTGC 
TTAAAAGAAC CTGAAAGATT ATGTACAGAC AAAGAACTTA TATTGGACCC TGTGCTTTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTT ATCTGTTATC CTCATGGCAT TAAAGAATGT 
ACCGAGGGGG ACAATCTGCA AAGACAGCAC ATTAAGCGTA TTCTTCAGAA TCTTGAGCAG 
TGGACACTGA GGCAATCCTG GTTAGAACTC CAGCTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCTGTGGC CGAAATGAAC AACTTACTGG ACAATATTGC AAAGGCAACA 
ATAGAGGTAT TCCAGCAGTC TGCAGACCTA AATAATTCTT CTAATTCTGG CATGAGCCTC 
TTCAACCCAA ACAGTATTGG AAGTGCTGAT ACAAGTAGCA CGAGACAGAA TGGAATAAAG 
ACATTCCTAA GTTCCTCCGA ACGCAGGGGT GTATGGTTGG TGGCCCCCCT CATCGCCAGG 
TTGCCAACTT CTGTGCAAGG AAGAGTGCTG AAAGCCGCTG GGGAAGAGCT GGAGAAGGGA 
CAGCACTTGG GTTCTTCTTC CAAAAAGGAA AGGGACAGAC AGAAACAGAA AAGTATGTCT 
CTTTTGAGTC AACAACCCTT CCTCTCACTG GTACTTACCT GCCTTAAGGG ACAAGATGAA 
CAAAGGGAAG GCCTCCTAAC ATCTCTCCAG AATCAAGTTA ACCAGATTTT AAGTAACTGG 
AGAGAAGAAC GATACCAAGA TGACATAAAA GCGCGGCAGA TGATGCACGA AGCATTGCAA 
CTCCGCCTAA ATTTGGTAGG AGGAATGTTT GACACGGTGC AGAGGAGCAC CCAGTGGACT 
ACAGACTGGG CCCTGCTACT CCTTCAGATC ATTACTTCAG GAACTGTTGA CATGCACACT 
AACAATGAAT TATTCACAAC AGTTCTTGAC ATGCTGGGTG TTTTAATCAA TGGAACGTTA 
GCCTCTGACC TATCAAATGC ATCCCCTGGG GGATCTGAAG AGAACAAGCG TGCATACATG 
AATTTAGTAA AGAAACTGAA AAAAGAGCTA GGAGACAAGC GATCAGAAAG TATTGACAAA 
GTTCGACAGT TACTACCTTT GCCGAAACAG ACATGTGATG TCATCACTTG TGAACCTATG 
GGTTCCTTGA TTGACACAAA AGGAAACAAA ATTGCTGGAT TTGACTCTAT AGATAAAAAA 
CAGGGTCTCC AGGTCTCTAC GAAGCAGAAG GTGTCCCCGT GGGACTTGTT TGAGGGTCAG 
AAGAACCCAG CTCCTTTGTC CTGGGCCTGG TTTGGGACAG TCCGAGTGGA CCGAAGAGTG 
ATCAAGTACG AGGAGCAGCA TCACCTCCTG CTGTATCACA CACACCCCAT GCCCAAGCCC 
CGCAGTTACT ACCTCCAGCC ACTGCCCCTG CCTCCTGAGG AGGAAGAGGA AGAGCCCACA 
TCTCCAGTTT CTCAGGAACC AGAAAGGAAG TCCGCTGAGC TGTCAGATCA GGGAAAAACC 
ACAACAGATG AAGAAAAGAA AACAAAAGGA AGGAAGCGCA AGACGAAATC TAGCTCAAGA 
GTTGATGAAT ATCCACAGAG CAACATATAC CGAGTGCCTC CTAATTACTC GCCTATCTCC 
TCCCAAATGA TGCACCATCC ACAGTCCACC TTGTGGGGTT ACAACCTCGT GGGCCAGCCC 
CAGCAGCCCG GCTTTTTCCT TCAGAACCAA TCTCTTACTC CAGGTGGCTC CAGATTGGAC 
CCTGCAGGCT CCTTTGTCCC AACCAACACC AAACAAGCTC TGTCAAACAT GCTACAGCGG 
CGCTCAGGCG CCATGATGCA GCCGCCTTCT CTTCATGCAA TCACATCGCA GCAGCAGTTG 
ATACAGATGA AGCTTCTGCA GCAGCAGCAG CAACAGCGAC TTCTCAGGCA AGCCCAGACT 
CGGCCTTTCC AACAGGGCCA GCCGGGGGAC CAGGCTGCTC TCTTTGCTGC GCAAGCACGG 
CCCTCCCCTC AGCTCCCTCA GTATCCAGGG CTGCAGCAAG CACAGACCAT GCCACAGGGC 
TATACAATGT ATGGGACACA GATGCCTTTG CAGCAGACAT CGCAGCAGCA GGCTGGCAGT 
GTGGTCCTGT CTCCCAGCTA TAACTCCAGA GCCTATCCGG CCGCACATTC CAACCCCGTG 
CTAATGGAAA GACTCAGACA GATTCAGCAG CAGCCGAGTG GCTATGTTCA GCAGCAGGCC 
TCGCCGTACC TGCAGCCCCT GACTGGCTCT CAGAGACTGA ACCATCAGGC TCTACAGCAG 
AGCCCTCTGG TGGGCGGGGG AATTGATGCT GTGCTGACTT CTGCACATCC AAACCTTCCC 
TCCGTGCCCC TGCCTCAGGA TCCCATGAGA CCCAGACAGC CGCAAGTTCG ACAGCAGCAG 
AGACTCCTCC AGATGCAGCA GCCCCAGCAG CCCCAGCCCC AGCAGCCTCC CCAGCCCCAG 
CAGTCCTCGC AGTCCCAGAG TCAGACCCTT GGTCTCCAAG CAATGCAGCC CCAGCAGCCC 
TTGTTTCCCA GGCAAGGCTT GCAGCAGACC CAGCAGCAGC AGCAGACGGC CGCCTTGGTG 
CGGCAGCTCC AGAAGCAGCT TTCCAGCAAC CAGCCACAGC AAGGAGTGAC TCCGTATGGG 
CATCCTTCAC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443728.3
CDS	130..6567
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLPSVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	NM_053002.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053002.5

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGAT TTTTTCTCCT 
ATGCCTGGAG AATCCTGTGA GAATGCCAAC ACTTCGTTGG GCAGAAGAAT GTCAGTTAAT 
TGTGAGAAGT TGGTGAAGAG GGAAAAGCCA AGGGAATTAA TTTTTCCATC TAATTATGAC 
CTCCTTCGCC ACTTACAGTA TGCAACACAT TTTCCTATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTC TATGGAGTCG GCAAAGAGCG TGATGAAGCA 
AGGCATCAGC TGAAGAAGAT TACCAAAGAT ATCCTGAAAA TTCTAAATAA GAAGAGCACC 
ACAGAGACAG GGGTTGGGGA CGAAGGACAA AAAGCCAGGA AGAACAAACA GGAGACATTT 
CCAACACTGG AGACTGTGTT CACTAAACTC CAGCTCCTTT CATATTTTGA TCAACATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC GTCAGGAACA 
TCCTATCATC TCCCTTTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTGAACATCA ACGGACTAAT TGACTTCGCA ATACAGTTAC TAAATGAACT GAGTGTTGTG 
GAAGCTGAAC TGCTCCTAAA ATCCTCCAGC CTGGCAGGAA GTTATACAAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGCTAT CACAGTTGTC TAATCTTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG GTTGTGTGGT GTGGTCAAGC ATGTCGTAAA CCCCTCAGAA 
TGTTCTTCCC CTGAAAGATG CATTTTAGCC TACCTCTATG ATCTCTATGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAATA ACGTGATGCC TGCAAATTCG AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC AGCCCGCAGC ATCAACTACT CAATGCTGGG CAAGATCCTC 
AGTGACAATG CGGCCAATCG CTACAGCTTT GTCTGCAATA CACTCATGAA TGTATGTATG 
GGCCATCAGG ATGCTGGCAG GATTAACGAC ATAGCCAATT TCTCCTCTGA GCTTACGGCT 
TGCTGCACTG TTCTTAGTTC AGAATGGCTG GGGGTTCTGA AGGCTCTTTG TTGTTCTTCA 
AATCACGTGT GGGGGTTTAA TGATGTACTT TGCACTGTAG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT TTCCCTGGAG 
GACGTCGTGC AGCATGTCGC ACTTCCCTCT CTTCTAGCAG CAGCTTGTGG GGATGCGGAC 
GCCGAGCCTG GGGCGAGAAT GACATGCCGA CTCTTGCTTC ATCTCTTCCG AGCTCCCCAG 
GCCTGCTTCT TACCTCAAGC AACGGGCAAA CCTTTCCCTG GAATAAGATC ATCTTGTGAT 
AGACACCTCT TAGCCGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAG 
AATGATGACT TCACCATGAG AGGTTTGCGA TGTGATGGGA ATGCTGATGA TATCTGGACT 
GCCTCACAAA ATCCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACTGC CAATTTAAGA 
GAATACGCTA GATATGTACT GAGGACTATC TGTCAACAGG AATGGGTAGG AGAGCATTGC 
TTAAAAGAAC CTGAAAGATT ATGTACAGAC AAAGAACTTA TATTGGACCC TGTGCTTTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTT ATCTGTTATC CTCATGGCAT TAAAGAATGT 
ACCGAGGGGG ACAATCTGCA AAGACAGCAC ATTAAGCGTA TTCTTCAGAA TCTTGAGCAG 
TGGACACTGA GGCAATCCTG GTTAGAACTC CAGCTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCTGTGGC CGAAATGAAC AACTTACTGG ACAATATTGC AAAGGCAACA 
ATAGAGGTAT TCCAGCAGTC TGCAGACCTA AATAATTCTT CTAATTCTGG CATGAGCCTC 
TTCAACCCAA ACAGTATTGG AAGTGCTGAT ACAAGTAGCA CGAGACAGAA TGGAATAAAG 
ACATTCCTAA GTTCCTCCGA ACGCAGGGGT GTATGGTTGG TGGCCCCCCT CATCGCCAGG 
TTGCCAACTT CTGTGCAAGG AAGAGTGCTG AAAGCCGCTG GGGAAGAGCT GGAGAAGGGA 
CAGCACTTGG GTTCTTCTTC CAAAAAGGAA AGGGACAGAC AGAAACAGAA AAGTATGTCT 
CTTTTGAGTC AACAACCCTT CCTCTCACTG GTACTTACCT GCCTTAAGGG ACAAGATGAA 
CAAAGGGAAG GCCTCCTAAC ATCTCTCCAG AATCAAGTTA ACCAGATTTT AAGTAACTGG 
AGAGAAGAAC GATACCAAGA TGACATAAAA GCGCGGCAGA TGATGCACGA AGCATTGCAA 
CTCCGCCTAA ATTTGGTAGG AGGAATGTTT GACACGGTGC AGAGGAGCAC CCAGTGGACT 
ACAGACTGGG CCCTGCTACT CCTTCAGATC ATTACTTCAG GAACTGTTGA CATGCACACT 
AACAATGAAT TATTCACAAC AGTTCTTGAC ATGCTGGGTG TTTTAATCAA TGGAACGTTA 
GCCTCTGACC TATCAAATGC ATCCCCTGGG GGATCTGAAG AGAACAAGCG TGCATACATG 
AATTTAGTAA AGAAACTGAA AAAAGAGCTA GGAGACAAGC GATCAGAAAG TATTGACAAA 
GTTCGACAGT TACTACCTTT GCCGAAACAG ACATGTGATG TCATCACTTG TGAACCTATG 
GGTTCCTTGA TTGACACAAA AGGAAACAAA ATTGCTGGAT TTGACTCTAT AGATAAAAAA 
CAGGGTCTCC AGGTCTCTAC GAAGCAGAAG GTGTCCCCGT GGGACTTGTT TGAGGGTCAG 
AAGAACCCAG CTCCTTTGTC CTGGGCCTGG TTTGGGACAG TCCGAGTGGA CCGAAGAGTG 
ATCAAGTACG AGGAGCAGCA TCACCTCCTG CTGTATCACA CACACCCCAT GCCCAAGCCC 
CGCAGTTACT ACCTCCAGCC ACTGCCCCTG CCTCCTGAGG AGGAAGAGGA AGAGCCCACA 
TCTCCAGTTT CTCAGGAACC AGAAAGGAAG TCCGCTGAGC TGTCAGATCA GGGAAAAACC 
ACAACAGATG AAGAAAAGAA AACAAAAGGA AGGAAGCGCA AGACGAAATC TAGCTCAAGA 
GTTGATGAAT ATCCACAGAG CAACATATAC CGAGTGCCTC CTAATTACTC GCCTATCTCC 
TCCCAAATGA TGCACCATCC ACAGTCCACC TTGTGGGGTT ACAACCTCGT GGGCCAGCCC 
CAGCAGCCCG GCTTTTTCCT TCAGAACCAA TCTCTTACTC CAGGTGGCTC CAGATTGGAC 
CCTGCAGGCT CCTTTGTCCC AACCAACACC AAACAAGCTC TGTCAAACAT GCTACAGCGG 
CGCTCAGGCG CCATGATGCA GCCGCCTTCT CTTCATGCAA TCACATCGCA GCAGCAGTTG 
ATACAGATGA AGCTTCTGCA GCAGCAGCAG CAACAGCGAC TTCTCAGGCA AGCCCAGACT 
CGGCCTTTCC AACAGGGCCA GCCGGGGGAC CAGGCTGCTC TCTTTGCTGC GCAAGCACGG 
CCCTCCCCTC AGCTCCCTCA GTATCCAGGG CTGCAGCAAG CACAGACCAT GCCACAGGGC 
TATACAATGT ATGGGACACA GATGCCTTTG CAGCAGACAT CGCAGCAGCA GGCTGGCAGT 
GTGGTCCTGT CTCCCAGCTA TAACTCCAGA GCCTATCCGG CCGCACATTC CAACCCCGTG 
CTAATGGAAA GACTCAGACA GATTCAGCAG CAGCCGAGTG GCTATGTTCA GCAGCAGGCC 
TCGCCGTACC TGCAGCCCCT GACTGGCTCT CAGAGACTGA ACCATCAGGC TCTACAGCAG 
AGCCCTCTGG TGGGCGGGGG AATTGATGCT GTGCTGACTT CTGCACATCC AAACCTTCCC 
TCCGTGCCCC TGCCTCAGGA TCCCATGAGA CCCAGACAGC CGCAAGTTCG ACAGCAGCAG 
AGACTCCTCC AGATGCAGCA GCCCCAGCAG CCCCAGCCCC AGCAGCCTCC CCAGCCCCAG 
CAGTCCTCGC AGTCCCAGAG TCAGACCCTT GGTCTCCAAG CAATGCAGCC CCAGCAGCCC 
TTGTTTCCCA GGCAAGGCTT GCAGCAGACC CAGCAGCAGC AGCAGACGGC CGCCTTGGTG 
CGGCAGCTCC AGAAGCAGCT TTCCAGCAAC CAGCCACAGC AAGGAGTGAC TCCGTATGGG 
CATCCTTCAC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_011512390.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510692.1 Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X5, mRNA.

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CloneID	OHu20944
Clone ID Related Accession (Same CDS sequence)	XM_011512390.2 , NM_053002.5 , XM_011512390.1
Accession Version	XM_011512390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGAT TTTTTCTCCT 
ATGCCTGGAG AATCCTGTGA GAATGCCAAC ACTTCGTTGG GCAGAAGAAT GTCAGTTAAT 
TGTGAGAAGT TGGTGAAGAG GGAAAAGCCA AGGGAATTAA TTTTTCCATC TAATTATGAC 
CTCCTTCGCC ACTTACAGTA TGCAACACAT TTTCCTATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTC TATGGAGTCG GCAAAGAGCG TGATGAAGCA 
AGGCATCAGC TGAAGAAGAT TACCAAAGAT ATCCTGAAAA TTCTAAATAA GAAGAGCACC 
ACAGAGACAG GGGTTGGGGA CGAAGGACAA AAAGCCAGGA AGAACAAACA GGAGACATTT 
CCAACACTGG AGACTGTGTT CACTAAACTC CAGCTCCTTT CATATTTTGA TCAACATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC GTCAGGAACA 
TCCTATCATC TCCCTTTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTGAACATCA ACGGACTAAT TGACTTCGCA ATACAGTTAC TAAATGAACT GAGTGTTGTG 
GAAGCTGAAC TGCTCCTAAA ATCCTCCAGC CTGGCAGGAA GTTATACAAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGCTAT CACAGTTGTC TAATCTTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG GTTGTGTGGT GTGGTCAAGC ATGTCGTAAA CCCCTCAGAA 
TGTTCTTCCC CTGAAAGATG CATTTTAGCC TACCTCTATG ATCTCTATGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAATA ACGTGATGCC TGCAAATTCG AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC AGCCCGCAGC ATCAACTACT CAATGCTGGG CAAGATCCTC 
AGTGACAATG CGGCCAATCG CTACAGCTTT GTCTGCAATA CACTCATGAA TGTATGTATG 
GGCCATCAGG ATGCTGGCAG GATTAACGAC ATAGCCAATT TCTCCTCTGA GCTTACGGCT 
TGCTGCACTG TTCTTAGTTC AGAATGGCTG GGGGTTCTGA AGGCTCTTTG TTGTTCTTCA 
AATCACGTGT GGGGGTTTAA TGATGTACTT TGCACTGTAG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT TTCCCTGGAG 
GACGTCGTGC AGCATGTCGC ACTTCCCTCT CTTCTAGCAG CAGCTTGTGG GGATGCGGAC 
GCCGAGCCTG GGGCGAGAAT GACATGCCGA CTCTTGCTTC ATCTCTTCCG AGCTCCCCAG 
GCCTGCTTCT TACCTCAAGC AACGGGCAAA CCTTTCCCTG GAATAAGATC ATCTTGTGAT 
AGACACCTCT TAGCCGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAG 
AATGATGACT TCACCATGAG AGGTTTGCGA TGTGATGGGA ATGCTGATGA TATCTGGACT 
GCCTCACAAA ATCCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACTGC CAATTTAAGA 
GAATACGCTA GATATGTACT GAGGACTATC TGTCAACAGG AATGGGTAGG AGAGCATTGC 
TTAAAAGAAC CTGAAAGATT ATGTACAGAC AAAGAACTTA TATTGGACCC TGTGCTTTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTT ATCTGTTATC CTCATGGCAT TAAAGAATGT 
ACCGAGGGGG ACAATCTGCA AAGACAGCAC ATTAAGCGTA TTCTTCAGAA TCTTGAGCAG 
TGGACACTGA GGCAATCCTG GTTAGAACTC CAGCTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCTGTGGC CGAAATGAAC AACTTACTGG ACAATATTGC AAAGGCAACA 
ATAGAGGTAT TCCAGCAGTC TGCAGACCTA AATAATTCTT CTAATTCTGG CATGAGCCTC 
TTCAACCCAA ACAGTATTGG AAGTGCTGAT ACAAGTAGCA CGAGACAGAA TGGAATAAAG 
ACATTCCTAA GTTCCTCCGA ACGCAGGGGT GTATGGTTGG TGGCCCCCCT CATCGCCAGG 
TTGCCAACTT CTGTGCAAGG AAGAGTGCTG AAAGCCGCTG GGGAAGAGCT GGAGAAGGGA 
CAGCACTTGG GTTCTTCTTC CAAAAAGGAA AGGGACAGAC AGAAACAGAA AAGTATGTCT 
CTTTTGAGTC AACAACCCTT CCTCTCACTG GTACTTACCT GCCTTAAGGG ACAAGATGAA 
CAAAGGGAAG GCCTCCTAAC ATCTCTCCAG AATCAAGTTA ACCAGATTTT AAGTAACTGG 
AGAGAAGAAC GATACCAAGA TGACATAAAA GCGCGGCAGA TGATGCACGA AGCATTGCAA 
CTCCGCCTAA ATTTGGTAGG AGGAATGTTT GACACGGTGC AGAGGAGCAC CCAGTGGACT 
ACAGACTGGG CCCTGCTACT CCTTCAGATC ATTACTTCAG GAACTGTTGA CATGCACACT 
AACAATGAAT TATTCACAAC AGTTCTTGAC ATGCTGGGTG TTTTAATCAA TGGAACGTTA 
GCCTCTGACC TATCAAATGC ATCCCCTGGG GGATCTGAAG AGAACAAGCG TGCATACATG 
AATTTAGTAA AGAAACTGAA AAAAGAGCTA GGAGACAAGC GATCAGAAAG TATTGACAAA 
GTTCGACAGT TACTACCTTT GCCGAAACAG ACATGTGATG TCATCACTTG TGAACCTATG 
GGTTCCTTGA TTGACACAAA AGGAAACAAA ATTGCTGGAT TTGACTCTAT AGATAAAAAA 
CAGGGTCTCC AGGTCTCTAC GAAGCAGAAG GTGTCCCCGT GGGACTTGTT TGAGGGTCAG 
AAGAACCCAG CTCCTTTGTC CTGGGCCTGG TTTGGGACAG TCCGAGTGGA CCGAAGAGTG 
ATCAAGTACG AGGAGCAGCA TCACCTCCTG CTGTATCACA CACACCCCAT GCCCAAGCCC 
CGCAGTTACT ACCTCCAGCC ACTGCCCCTG CCTCCTGAGG AGGAAGAGGA AGAGCCCACA 
TCTCCAGTTT CTCAGGAACC AGAAAGGAAG TCCGCTGAGC TGTCAGATCA GGGAAAAACC 
ACAACAGATG AAGAAAAGAA AACAAAAGGA AGGAAGCGCA AGACGAAATC TAGCTCAAGA 
GTTGATGAAT ATCCACAGAG CAACATATAC CGAGTGCCTC CTAATTACTC GCCTATCTCC 
TCCCAAATGA TGCACCATCC ACAGTCCACC TTGTGGGGTT ACAACCTCGT GGGCCAGCCC 
CAGCAGCCCG GCTTTTTCCT TCAGAACCAA TCTCTTACTC CAGGTGGCTC CAGATTGGAC 
CCTGCAGGCT CCTTTGTCCC AACCAACACC AAACAAGCTC TGTCAAACAT GCTACAGCGG 
CGCTCAGGCG CCATGATGCA GCCGCCTTCT CTTCATGCAA TCACATCGCA GCAGCAGTTG 
ATACAGATGA AGCTTCTGCA GCAGCAGCAG CAACAGCGAC TTCTCAGGCA AGCCCAGACT 
CGGCCTTTCC AACAGGGCCA GCCGGGGGAC CAGGCTGCTC TCTTTGCTGC GCAAGCACGG 
CCCTCCCCTC AGCTCCCTCA GTATCCAGGG CTGCAGCAAG CACAGACCAT GCCACAGGGC 
TATACAATGT ATGGGACACA GATGCCTTTG CAGCAGACAT CGCAGCAGCA GGCTGGCAGT 
GTGGTCCTGT CTCCCAGCTA TAACTCCAGA GCCTATCCGG CCGCACATTC CAACCCCGTG 
CTAATGGAAA GACTCAGACA GATTCAGCAG CAGCCGAGTG GCTATGTTCA GCAGCAGGCC 
TCGCCGTACC TGCAGCCCCT GACTGGCTCT CAGAGACTGA ACCATCAGGC TCTACAGCAG 
AGCCCTCTGG TGGGCGGGGG AATTGATGCT GTGCTGACTT CTGCACATCC AAACCTTCCC 
TCCGTGCCCC TGCCTCAGGA TCCCATGAGA CCCAGACAGC CGCAAGTTCG ACAGCAGCAG 
AGACTCCTCC AGATGCAGCA GCCCCAGCAG CCCCAGCCCC AGCAGCCTCC CCAGCCCCAG 
CAGTCCTCGC AGTCCCAGAG TCAGACCCTT GGTCTCCAAG CAATGCAGCC CCAGCAGCCC 
TTGTTTCCCA GGCAAGGCTT GCAGCAGACC CAGCAGCAGC AGCAGACGGC CGCCTTGGTG 
CGGCAGCTCC AGAAGCAGCT TTCCAGCAAC CAGCCACAGC AAGGAGTGAC TCCGTATGGG 
CATCCTTCAC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510692.1
CDS	402..6839
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLPSVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_011512390.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512390.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGAT TTTTTCTCCT 
ATGCCTGGAG AATCCTGTGA GAATGCCAAC ACTTCGTTGG GCAGAAGAAT GTCAGTTAAT 
TGTGAGAAGT TGGTGAAGAG GGAAAAGCCA AGGGAATTAA TTTTTCCATC TAATTATGAC 
CTCCTTCGCC ACTTACAGTA TGCAACACAT TTTCCTATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTC TATGGAGTCG GCAAAGAGCG TGATGAAGCA 
AGGCATCAGC TGAAGAAGAT TACCAAAGAT ATCCTGAAAA TTCTAAATAA GAAGAGCACC 
ACAGAGACAG GGGTTGGGGA CGAAGGACAA AAAGCCAGGA AGAACAAACA GGAGACATTT 
CCAACACTGG AGACTGTGTT CACTAAACTC CAGCTCCTTT CATATTTTGA TCAACATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC GTCAGGAACA 
TCCTATCATC TCCCTTTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTGAACATCA ACGGACTAAT TGACTTCGCA ATACAGTTAC TAAATGAACT GAGTGTTGTG 
GAAGCTGAAC TGCTCCTAAA ATCCTCCAGC CTGGCAGGAA GTTATACAAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGCTAT CACAGTTGTC TAATCTTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG GTTGTGTGGT GTGGTCAAGC ATGTCGTAAA CCCCTCAGAA 
TGTTCTTCCC CTGAAAGATG CATTTTAGCC TACCTCTATG ATCTCTATGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAATA ACGTGATGCC TGCAAATTCG AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC AGCCCGCAGC ATCAACTACT CAATGCTGGG CAAGATCCTC 
AGTGACAATG CGGCCAATCG CTACAGCTTT GTCTGCAATA CACTCATGAA TGTATGTATG 
GGCCATCAGG ATGCTGGCAG GATTAACGAC ATAGCCAATT TCTCCTCTGA GCTTACGGCT 
TGCTGCACTG TTCTTAGTTC AGAATGGCTG GGGGTTCTGA AGGCTCTTTG TTGTTCTTCA 
AATCACGTGT GGGGGTTTAA TGATGTACTT TGCACTGTAG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT TTCCCTGGAG 
GACGTCGTGC AGCATGTCGC ACTTCCCTCT CTTCTAGCAG CAGCTTGTGG GGATGCGGAC 
GCCGAGCCTG GGGCGAGAAT GACATGCCGA CTCTTGCTTC ATCTCTTCCG AGCTCCCCAG 
GCCTGCTTCT TACCTCAAGC AACGGGCAAA CCTTTCCCTG GAATAAGATC ATCTTGTGAT 
AGACACCTCT TAGCCGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAG 
AATGATGACT TCACCATGAG AGGTTTGCGA TGTGATGGGA ATGCTGATGA TATCTGGACT 
GCCTCACAAA ATCCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACTGC CAATTTAAGA 
GAATACGCTA GATATGTACT GAGGACTATC TGTCAACAGG AATGGGTAGG AGAGCATTGC 
TTAAAAGAAC CTGAAAGATT ATGTACAGAC AAAGAACTTA TATTGGACCC TGTGCTTTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTT ATCTGTTATC CTCATGGCAT TAAAGAATGT 
ACCGAGGGGG ACAATCTGCA AAGACAGCAC ATTAAGCGTA TTCTTCAGAA TCTTGAGCAG 
TGGACACTGA GGCAATCCTG GTTAGAACTC CAGCTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCTGTGGC CGAAATGAAC AACTTACTGG ACAATATTGC AAAGGCAACA 
ATAGAGGTAT TCCAGCAGTC TGCAGACCTA AATAATTCTT CTAATTCTGG CATGAGCCTC 
TTCAACCCAA ACAGTATTGG AAGTGCTGAT ACAAGTAGCA CGAGACAGAA TGGAATAAAG 
ACATTCCTAA GTTCCTCCGA ACGCAGGGGT GTATGGTTGG TGGCCCCCCT CATCGCCAGG 
TTGCCAACTT CTGTGCAAGG AAGAGTGCTG AAAGCCGCTG GGGAAGAGCT GGAGAAGGGA 
CAGCACTTGG GTTCTTCTTC CAAAAAGGAA AGGGACAGAC AGAAACAGAA AAGTATGTCT 
CTTTTGAGTC AACAACCCTT CCTCTCACTG GTACTTACCT GCCTTAAGGG ACAAGATGAA 
CAAAGGGAAG GCCTCCTAAC ATCTCTCCAG AATCAAGTTA ACCAGATTTT AAGTAACTGG 
AGAGAAGAAC GATACCAAGA TGACATAAAA GCGCGGCAGA TGATGCACGA AGCATTGCAA 
CTCCGCCTAA ATTTGGTAGG AGGAATGTTT GACACGGTGC AGAGGAGCAC CCAGTGGACT 
ACAGACTGGG CCCTGCTACT CCTTCAGATC ATTACTTCAG GAACTGTTGA CATGCACACT 
AACAATGAAT TATTCACAAC AGTTCTTGAC ATGCTGGGTG TTTTAATCAA TGGAACGTTA 
GCCTCTGACC TATCAAATGC ATCCCCTGGG GGATCTGAAG AGAACAAGCG TGCATACATG 
AATTTAGTAA AGAAACTGAA AAAAGAGCTA GGAGACAAGC GATCAGAAAG TATTGACAAA 
GTTCGACAGT TACTACCTTT GCCGAAACAG ACATGTGATG TCATCACTTG TGAACCTATG 
GGTTCCTTGA TTGACACAAA AGGAAACAAA ATTGCTGGAT TTGACTCTAT AGATAAAAAA 
CAGGGTCTCC AGGTCTCTAC GAAGCAGAAG GTGTCCCCGT GGGACTTGTT TGAGGGTCAG 
AAGAACCCAG CTCCTTTGTC CTGGGCCTGG TTTGGGACAG TCCGAGTGGA CCGAAGAGTG 
ATCAAGTACG AGGAGCAGCA TCACCTCCTG CTGTATCACA CACACCCCAT GCCCAAGCCC 
CGCAGTTACT ACCTCCAGCC ACTGCCCCTG CCTCCTGAGG AGGAAGAGGA AGAGCCCACA 
TCTCCAGTTT CTCAGGAACC AGAAAGGAAG TCCGCTGAGC TGTCAGATCA GGGAAAAACC 
ACAACAGATG AAGAAAAGAA AACAAAAGGA AGGAAGCGCA AGACGAAATC TAGCTCAAGA 
GTTGATGAAT ATCCACAGAG CAACATATAC CGAGTGCCTC CTAATTACTC GCCTATCTCC 
TCCCAAATGA TGCACCATCC ACAGTCCACC TTGTGGGGTT ACAACCTCGT GGGCCAGCCC 
CAGCAGCCCG GCTTTTTCCT TCAGAACCAA TCTCTTACTC CAGGTGGCTC CAGATTGGAC 
CCTGCAGGCT CCTTTGTCCC AACCAACACC AAACAAGCTC TGTCAAACAT GCTACAGCGG 
CGCTCAGGCG CCATGATGCA GCCGCCTTCT CTTCATGCAA TCACATCGCA GCAGCAGTTG 
ATACAGATGA AGCTTCTGCA GCAGCAGCAG CAACAGCGAC TTCTCAGGCA AGCCCAGACT 
CGGCCTTTCC AACAGGGCCA GCCGGGGGAC CAGGCTGCTC TCTTTGCTGC GCAAGCACGG 
CCCTCCCCTC AGCTCCCTCA GTATCCAGGG CTGCAGCAAG CACAGACCAT GCCACAGGGC 
TATACAATGT ATGGGACACA GATGCCTTTG CAGCAGACAT CGCAGCAGCA GGCTGGCAGT 
GTGGTCCTGT CTCCCAGCTA TAACTCCAGA GCCTATCCGG CCGCACATTC CAACCCCGTG 
CTAATGGAAA GACTCAGACA GATTCAGCAG CAGCCGAGTG GCTATGTTCA GCAGCAGGCC 
TCGCCGTACC TGCAGCCCCT GACTGGCTCT CAGAGACTGA ACCATCAGGC TCTACAGCAG 
AGCCCTCTGG TGGGCGGGGG AATTGATGCT GTGCTGACTT CTGCACATCC AAACCTTCCC 
TCCGTGCCCC TGCCTCAGGA TCCCATGAGA CCCAGACAGC CGCAAGTTCG ACAGCAGCAG 
AGACTCCTCC AGATGCAGCA GCCCCAGCAG CCCCAGCCCC AGCAGCCTCC CCAGCCCCAG 
CAGTCCTCGC AGTCCCAGAG TCAGACCCTT GGTCTCCAAG CAATGCAGCC CCAGCAGCCC 
TTGTTTCCCA GGCAAGGCTT GCAGCAGACC CAGCAGCAGC AGCAGACGGC CGCCTTGGTG 
CGGCAGCTCC AGAAGCAGCT TTCCAGCAAC CAGCCACAGC AAGGAGTGAC TCCGTATGGG 
CATCCTTCAC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_011512390.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510692.1 Homo sapiens mediator complex subunit 12-like (MED12L), transcript variant X6, mRNA.

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CloneID	OHu20944
Clone ID Related Accession (Same CDS sequence)	XM_011512390.2 , NM_053002.5 , XM_011512390.1
Accession Version	XM_011512390.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
Comment

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGAT TTTTTCTCCT 
ATGCCTGGAG AATCCTGTGA GAATGCCAAC ACTTCGTTGG GCAGAAGAAT GTCAGTTAAT 
TGTGAGAAGT TGGTGAAGAG GGAAAAGCCA AGGGAATTAA TTTTTCCATC TAATTATGAC 
CTCCTTCGCC ACTTACAGTA TGCAACACAT TTTCCTATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTC TATGGAGTCG GCAAAGAGCG TGATGAAGCA 
AGGCATCAGC TGAAGAAGAT TACCAAAGAT ATCCTGAAAA TTCTAAATAA GAAGAGCACC 
ACAGAGACAG GGGTTGGGGA CGAAGGACAA AAAGCCAGGA AGAACAAACA GGAGACATTT 
CCAACACTGG AGACTGTGTT CACTAAACTC CAGCTCCTTT CATATTTTGA TCAACATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC GTCAGGAACA 
TCCTATCATC TCCCTTTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTGAACATCA ACGGACTAAT TGACTTCGCA ATACAGTTAC TAAATGAACT GAGTGTTGTG 
GAAGCTGAAC TGCTCCTAAA ATCCTCCAGC CTGGCAGGAA GTTATACAAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGCTAT CACAGTTGTC TAATCTTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG GTTGTGTGGT GTGGTCAAGC ATGTCGTAAA CCCCTCAGAA 
TGTTCTTCCC CTGAAAGATG CATTTTAGCC TACCTCTATG ATCTCTATGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAATA ACGTGATGCC TGCAAATTCG AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC AGCCCGCAGC ATCAACTACT CAATGCTGGG CAAGATCCTC 
AGTGACAATG CGGCCAATCG CTACAGCTTT GTCTGCAATA CACTCATGAA TGTATGTATG 
GGCCATCAGG ATGCTGGCAG GATTAACGAC ATAGCCAATT TCTCCTCTGA GCTTACGGCT 
TGCTGCACTG TTCTTAGTTC AGAATGGCTG GGGGTTCTGA AGGCTCTTTG TTGTTCTTCA 
AATCACGTGT GGGGGTTTAA TGATGTACTT TGCACTGTAG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT TTCCCTGGAG 
GACGTCGTGC AGCATGTCGC ACTTCCCTCT CTTCTAGCAG CAGCTTGTGG GGATGCGGAC 
GCCGAGCCTG GGGCGAGAAT GACATGCCGA CTCTTGCTTC ATCTCTTCCG AGCTCCCCAG 
GCCTGCTTCT TACCTCAAGC AACGGGCAAA CCTTTCCCTG GAATAAGATC ATCTTGTGAT 
AGACACCTCT TAGCCGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAG 
AATGATGACT TCACCATGAG AGGTTTGCGA TGTGATGGGA ATGCTGATGA TATCTGGACT 
GCCTCACAAA ATCCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACTGC CAATTTAAGA 
GAATACGCTA GATATGTACT GAGGACTATC TGTCAACAGG AATGGGTAGG AGAGCATTGC 
TTAAAAGAAC CTGAAAGATT ATGTACAGAC AAAGAACTTA TATTGGACCC TGTGCTTTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTT ATCTGTTATC CTCATGGCAT TAAAGAATGT 
ACCGAGGGGG ACAATCTGCA AAGACAGCAC ATTAAGCGTA TTCTTCAGAA TCTTGAGCAG 
TGGACACTGA GGCAATCCTG GTTAGAACTC CAGCTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCTGTGGC CGAAATGAAC AACTTACTGG ACAATATTGC AAAGGCAACA 
ATAGAGGTAT TCCAGCAGTC TGCAGACCTA AATAATTCTT CTAATTCTGG CATGAGCCTC 
TTCAACCCAA ACAGTATTGG AAGTGCTGAT ACAAGTAGCA CGAGACAGAA TGGAATAAAG 
ACATTCCTAA GTTCCTCCGA ACGCAGGGGT GTATGGTTGG TGGCCCCCCT CATCGCCAGG 
TTGCCAACTT CTGTGCAAGG AAGAGTGCTG AAAGCCGCTG GGGAAGAGCT GGAGAAGGGA 
CAGCACTTGG GTTCTTCTTC CAAAAAGGAA AGGGACAGAC AGAAACAGAA AAGTATGTCT 
CTTTTGAGTC AACAACCCTT CCTCTCACTG GTACTTACCT GCCTTAAGGG ACAAGATGAA 
CAAAGGGAAG GCCTCCTAAC ATCTCTCCAG AATCAAGTTA ACCAGATTTT AAGTAACTGG 
AGAGAAGAAC GATACCAAGA TGACATAAAA GCGCGGCAGA TGATGCACGA AGCATTGCAA 
CTCCGCCTAA ATTTGGTAGG AGGAATGTTT GACACGGTGC AGAGGAGCAC CCAGTGGACT 
ACAGACTGGG CCCTGCTACT CCTTCAGATC ATTACTTCAG GAACTGTTGA CATGCACACT 
AACAATGAAT TATTCACAAC AGTTCTTGAC ATGCTGGGTG TTTTAATCAA TGGAACGTTA 
GCCTCTGACC TATCAAATGC ATCCCCTGGG GGATCTGAAG AGAACAAGCG TGCATACATG 
AATTTAGTAA AGAAACTGAA AAAAGAGCTA GGAGACAAGC GATCAGAAAG TATTGACAAA 
GTTCGACAGT TACTACCTTT GCCGAAACAG ACATGTGATG TCATCACTTG TGAACCTATG 
GGTTCCTTGA TTGACACAAA AGGAAACAAA ATTGCTGGAT TTGACTCTAT AGATAAAAAA 
CAGGGTCTCC AGGTCTCTAC GAAGCAGAAG GTGTCCCCGT GGGACTTGTT TGAGGGTCAG 
AAGAACCCAG CTCCTTTGTC CTGGGCCTGG TTTGGGACAG TCCGAGTGGA CCGAAGAGTG 
ATCAAGTACG AGGAGCAGCA TCACCTCCTG CTGTATCACA CACACCCCAT GCCCAAGCCC 
CGCAGTTACT ACCTCCAGCC ACTGCCCCTG CCTCCTGAGG AGGAAGAGGA AGAGCCCACA 
TCTCCAGTTT CTCAGGAACC AGAAAGGAAG TCCGCTGAGC TGTCAGATCA GGGAAAAACC 
ACAACAGATG AAGAAAAGAA AACAAAAGGA AGGAAGCGCA AGACGAAATC TAGCTCAAGA 
GTTGATGAAT ATCCACAGAG CAACATATAC CGAGTGCCTC CTAATTACTC GCCTATCTCC 
TCCCAAATGA TGCACCATCC ACAGTCCACC TTGTGGGGTT ACAACCTCGT GGGCCAGCCC 
CAGCAGCCCG GCTTTTTCCT TCAGAACCAA TCTCTTACTC CAGGTGGCTC CAGATTGGAC 
CCTGCAGGCT CCTTTGTCCC AACCAACACC AAACAAGCTC TGTCAAACAT GCTACAGCGG 
CGCTCAGGCG CCATGATGCA GCCGCCTTCT CTTCATGCAA TCACATCGCA GCAGCAGTTG 
ATACAGATGA AGCTTCTGCA GCAGCAGCAG CAACAGCGAC TTCTCAGGCA AGCCCAGACT 
CGGCCTTTCC AACAGGGCCA GCCGGGGGAC CAGGCTGCTC TCTTTGCTGC GCAAGCACGG 
CCCTCCCCTC AGCTCCCTCA GTATCCAGGG CTGCAGCAAG CACAGACCAT GCCACAGGGC 
TATACAATGT ATGGGACACA GATGCCTTTG CAGCAGACAT CGCAGCAGCA GGCTGGCAGT 
GTGGTCCTGT CTCCCAGCTA TAACTCCAGA GCCTATCCGG CCGCACATTC CAACCCCGTG 
CTAATGGAAA GACTCAGACA GATTCAGCAG CAGCCGAGTG GCTATGTTCA GCAGCAGGCC 
TCGCCGTACC TGCAGCCCCT GACTGGCTCT CAGAGACTGA ACCATCAGGC TCTACAGCAG 
AGCCCTCTGG TGGGCGGGGG AATTGATGCT GTGCTGACTT CTGCACATCC AAACCTTCCC 
TCCGTGCCCC TGCCTCAGGA TCCCATGAGA CCCAGACAGC CGCAAGTTCG ACAGCAGCAG 
AGACTCCTCC AGATGCAGCA GCCCCAGCAG CCCCAGCCCC AGCAGCCTCC CCAGCCCCAG 
CAGTCCTCGC AGTCCCAGAG TCAGACCCTT GGTCTCCAAG CAATGCAGCC CCAGCAGCCC 
TTGTTTCCCA GGCAAGGCTT GCAGCAGACC CAGCAGCAGC AGCAGACGGC CGCCTTGGTG 
CGGCAGCTCC AGAAGCAGCT TTCCAGCAAC CAGCCACAGC AAGGAGTGAC TCCGTATGGG 
CATCCTTCAC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011510692.1

Translation

MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLPSVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_011512390.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator complex subunit 12-like (MED12L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512390.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGAT TTTTTCTCCT 
ATGCCTGGAG AATCCTGTGA GAATGCCAAC ACTTCGTTGG GCAGAAGAAT GTCAGTTAAT 
TGTGAGAAGT TGGTGAAGAG GGAAAAGCCA AGGGAATTAA TTTTTCCATC TAATTATGAC 
CTCCTTCGCC ACTTACAGTA TGCAACACAT TTTCCTATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTC TATGGAGTCG GCAAAGAGCG TGATGAAGCA 
AGGCATCAGC TGAAGAAGAT TACCAAAGAT ATCCTGAAAA TTCTAAATAA GAAGAGCACC 
ACAGAGACAG GGGTTGGGGA CGAAGGACAA AAAGCCAGGA AGAACAAACA GGAGACATTT 
CCAACACTGG AGACTGTGTT CACTAAACTC CAGCTCCTTT CATATTTTGA TCAACATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC GTCAGGAACA 
TCCTATCATC TCCCTTTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTGAACATCA ACGGACTAAT TGACTTCGCA ATACAGTTAC TAAATGAACT GAGTGTTGTG 
GAAGCTGAAC TGCTCCTAAA ATCCTCCAGC CTGGCAGGAA GTTATACAAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGCTAT CACAGTTGTC TAATCTTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG GTTGTGTGGT GTGGTCAAGC ATGTCGTAAA CCCCTCAGAA 
TGTTCTTCCC CTGAAAGATG CATTTTAGCC TACCTCTATG ATCTCTATGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAATA ACGTGATGCC TGCAAATTCG AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC AGCCCGCAGC ATCAACTACT CAATGCTGGG CAAGATCCTC 
AGTGACAATG CGGCCAATCG CTACAGCTTT GTCTGCAATA CACTCATGAA TGTATGTATG 
GGCCATCAGG ATGCTGGCAG GATTAACGAC ATAGCCAATT TCTCCTCTGA GCTTACGGCT 
TGCTGCACTG TTCTTAGTTC AGAATGGCTG GGGGTTCTGA AGGCTCTTTG TTGTTCTTCA 
AATCACGTGT GGGGGTTTAA TGATGTACTT TGCACTGTAG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT TTCCCTGGAG 
GACGTCGTGC AGCATGTCGC ACTTCCCTCT CTTCTAGCAG CAGCTTGTGG GGATGCGGAC 
GCCGAGCCTG GGGCGAGAAT GACATGCCGA CTCTTGCTTC ATCTCTTCCG AGCTCCCCAG 
GCCTGCTTCT TACCTCAAGC AACGGGCAAA CCTTTCCCTG GAATAAGATC ATCTTGTGAT 
AGACACCTCT TAGCCGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAG 
AATGATGACT TCACCATGAG AGGTTTGCGA TGTGATGGGA ATGCTGATGA TATCTGGACT 
GCCTCACAAA ATCCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACTGC CAATTTAAGA 
GAATACGCTA GATATGTACT GAGGACTATC TGTCAACAGG AATGGGTAGG AGAGCATTGC 
TTAAAAGAAC CTGAAAGATT ATGTACAGAC AAAGAACTTA TATTGGACCC TGTGCTTTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTT ATCTGTTATC CTCATGGCAT TAAAGAATGT 
ACCGAGGGGG ACAATCTGCA AAGACAGCAC ATTAAGCGTA TTCTTCAGAA TCTTGAGCAG 
TGGACACTGA GGCAATCCTG GTTAGAACTC CAGCTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCTGTGGC CGAAATGAAC AACTTACTGG ACAATATTGC AAAGGCAACA 
ATAGAGGTAT TCCAGCAGTC TGCAGACCTA AATAATTCTT CTAATTCTGG CATGAGCCTC 
TTCAACCCAA ACAGTATTGG AAGTGCTGAT ACAAGTAGCA CGAGACAGAA TGGAATAAAG 
ACATTCCTAA GTTCCTCCGA ACGCAGGGGT GTATGGTTGG TGGCCCCCCT CATCGCCAGG 
TTGCCAACTT CTGTGCAAGG AAGAGTGCTG AAAGCCGCTG GGGAAGAGCT GGAGAAGGGA 
CAGCACTTGG GTTCTTCTTC CAAAAAGGAA AGGGACAGAC AGAAACAGAA AAGTATGTCT 
CTTTTGAGTC AACAACCCTT CCTCTCACTG GTACTTACCT GCCTTAAGGG ACAAGATGAA 
CAAAGGGAAG GCCTCCTAAC ATCTCTCCAG AATCAAGTTA ACCAGATTTT AAGTAACTGG 
AGAGAAGAAC GATACCAAGA TGACATAAAA GCGCGGCAGA TGATGCACGA AGCATTGCAA 
CTCCGCCTAA ATTTGGTAGG AGGAATGTTT GACACGGTGC AGAGGAGCAC CCAGTGGACT 
ACAGACTGGG CCCTGCTACT CCTTCAGATC ATTACTTCAG GAACTGTTGA CATGCACACT 
AACAATGAAT TATTCACAAC AGTTCTTGAC ATGCTGGGTG TTTTAATCAA TGGAACGTTA 
GCCTCTGACC TATCAAATGC ATCCCCTGGG GGATCTGAAG AGAACAAGCG TGCATACATG 
AATTTAGTAA AGAAACTGAA AAAAGAGCTA GGAGACAAGC GATCAGAAAG TATTGACAAA 
GTTCGACAGT TACTACCTTT GCCGAAACAG ACATGTGATG TCATCACTTG TGAACCTATG 
GGTTCCTTGA TTGACACAAA AGGAAACAAA ATTGCTGGAT TTGACTCTAT AGATAAAAAA 
CAGGGTCTCC AGGTCTCTAC GAAGCAGAAG GTGTCCCCGT GGGACTTGTT TGAGGGTCAG 
AAGAACCCAG CTCCTTTGTC CTGGGCCTGG TTTGGGACAG TCCGAGTGGA CCGAAGAGTG 
ATCAAGTACG AGGAGCAGCA TCACCTCCTG CTGTATCACA CACACCCCAT GCCCAAGCCC 
CGCAGTTACT ACCTCCAGCC ACTGCCCCTG CCTCCTGAGG AGGAAGAGGA AGAGCCCACA 
TCTCCAGTTT CTCAGGAACC AGAAAGGAAG TCCGCTGAGC TGTCAGATCA GGGAAAAACC 
ACAACAGATG AAGAAAAGAA AACAAAAGGA AGGAAGCGCA AGACGAAATC TAGCTCAAGA 
GTTGATGAAT ATCCACAGAG CAACATATAC CGAGTGCCTC CTAATTACTC GCCTATCTCC 
TCCCAAATGA TGCACCATCC ACAGTCCACC TTGTGGGGTT ACAACCTCGT GGGCCAGCCC 
CAGCAGCCCG GCTTTTTCCT TCAGAACCAA TCTCTTACTC CAGGTGGCTC CAGATTGGAC 
CCTGCAGGCT CCTTTGTCCC AACCAACACC AAACAAGCTC TGTCAAACAT GCTACAGCGG 
CGCTCAGGCG CCATGATGCA GCCGCCTTCT CTTCATGCAA TCACATCGCA GCAGCAGTTG 
ATACAGATGA AGCTTCTGCA GCAGCAGCAG CAACAGCGAC TTCTCAGGCA AGCCCAGACT 
CGGCCTTTCC AACAGGGCCA GCCGGGGGAC CAGGCTGCTC TCTTTGCTGC GCAAGCACGG 
CCCTCCCCTC AGCTCCCTCA GTATCCAGGG CTGCAGCAAG CACAGACCAT GCCACAGGGC 
TATACAATGT ATGGGACACA GATGCCTTTG CAGCAGACAT CGCAGCAGCA GGCTGGCAGT 
GTGGTCCTGT CTCCCAGCTA TAACTCCAGA GCCTATCCGG CCGCACATTC CAACCCCGTG 
CTAATGGAAA GACTCAGACA GATTCAGCAG CAGCCGAGTG GCTATGTTCA GCAGCAGGCC 
TCGCCGTACC TGCAGCCCCT GACTGGCTCT CAGAGACTGA ACCATCAGGC TCTACAGCAG 
AGCCCTCTGG TGGGCGGGGG AATTGATGCT GTGCTGACTT CTGCACATCC AAACCTTCCC 
TCCGTGCCCC TGCCTCAGGA TCCCATGAGA CCCAGACAGC CGCAAGTTCG ACAGCAGCAG 
AGACTCCTCC AGATGCAGCA GCCCCAGCAG CCCCAGCCCC AGCAGCCTCC CCAGCCCCAG 
CAGTCCTCGC AGTCCCAGAG TCAGACCCTT GGTCTCCAAG CAATGCAGCC CCAGCAGCCC 
TTGTTTCCCA GGCAAGGCTT GCAGCAGACC CAGCAGCAGC AGCAGACGGC CGCCTTGGTG 
CGGCAGCTCC AGAAGCAGCT TTCCAGCAAC CAGCCACAGC AAGGAGTGAC TCCGTATGGG 
CATCCTTCAC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_006713487.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713550.1 Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X3, mRNA.

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CloneID	OHu37689
Clone ID Related Accession (Same CDS sequence)	XM_006713487.3
Accession Version	XM_006713487.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAGT GTCAGCTCTT TAAAGAATGA TGACTTCACC 
ATGAGAGGTT TGCGATGTGA TGGGAATGCT GATGATATCT GGACTGCCTC ACAAAATCCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACTGCCAATT TAAGAGAATA CGCTAGATAT 
GTACTGAGGA CTATCTGTCA ACAGGAATGG GTAGGAGAGC ATTGCTTAAA AGAACCTGAA 
AGATTATGTA CAGACAAAGA ACTTATATTG GACCCTGTGC TTTCAAATAT GCAAGCACAG 
AAATTACTGC AGCTTATCTG TTATCCTCAT GGCATTAAAG AATGTACCGA GGGGGACAAT 
CTGCAAAGAC AGCACATTAA GCGTATTCTT CAGAATCTTG AGCAGTGGAC ACTGAGGCAA 
TCCTGGTTAG AACTCCAGCT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCT 
GTGGCCGAAA TGAACAACTT ACTGGACAAT ATTGCAAAGG CAACAATAGA GGTATTCCAG 
CAGTCTGCAG ACCTAAATAA TTCTTCTAAT TCTGGCATGA GCCTCTTCAA CCCAAACAGT 
ATTGGAAGTG CTGATACAAG TAGCACGAGA CAGAATGGAA TAAAGACATT CCTAAGTTCC 
TCCGAACGCA GGGGTGTATG GTTGGTGGCC CCCCTCATCG CCAGGTTGCC AACTTCTGTG 
CAAGGAAGAG TGCTGAAAGC CGCTGGGGAA GAGCTGGAGA AGGGACAGCA CTTGGGTTCT 
TCTTCCAAAA AGGAAAGGGA CAGACAGAAA CAGAAAAGTA TGTCTCTTTT GAGTCAACAA 
CCCTTCCTCT CACTGGTACT TACCTGCCTT AAGGGACAAG ATGAACAAAG GGAAGGCCTC 
CTAACATCTC TCCAGAATCA AGTTAACCAG ATTTTAAGTA ACTGGAGAGA AGAACGATAC 
CAAGATGACA TAAAAGCGCG GCAGATGATG CACGAAGCAT TGCAACTCCG CCTAAATTTG 
GTAGGAGGAA TGTTTGACAC GGTGCAGAGG AGCACCCAGT GGACTACAGA CTGGGCCCTG 
CTACTCCTTC AGATCATTAC TTCAGGAACT GTTGACATGC ACACTAACAA TGAATTATTC 
ACAACAGTTC TTGACATGCT GGGTGTTTTA ATCAATGGAA CGTTAGCCTC TGACCTATCA 
AATGCATCCC CTGGGGGATC TGAAGAGAAC AAGCGTGCAT ACATGAATTT AGTAAAGAAA 
CTGAAAAAAG AGCTAGGAGA CAAGCGATCA GAAAGTATTG ACAAAGTTCG ACAGTTACTA 
CCTTTGCCGA AACAGACATG TGATGTCATC ACTTGTGAAC CTATGGGTTC CTTGATTGAC 
ACAAAAGGAA ACAAAATTGC TGGATTTGAC TCTATAGATA AAAAACAGGG TCTCCAGGTC 
TCTACGAAGC AGAAGGTGTC CCCGTGGGAC TTGTTTGAGG GTCAGAAGAA CCCAGCTCCT 
TTGTCCTGGG CCTGGTTTGG GACAGTCCGA GTGGACCGAA GAGTGATCAA GTACGAGGAG 
CAGCATCACC TCCTGCTGTA TCACACACAC CCCATGCCCA AGCCCCGCAG TTACTACCTC 
CAGCCACTGC CCCTGCCTCC TGAGGAGGAA GAGGAAGAGC CCACATCTCC AGTTTCTCAG 
GAACCAGAAA GGAAGTCCGC TGAGCTGTCA GATCAGGGAA AAACCACAAC AGATGAAGAA 
AAGAAAACAA AAGGAAGGAA GCGCAAGACG AAATCTAGCT CAAGAGTTGA TGAATATCCA 
CAGAGCAACA TATACCGAGT GCCTCCTAAT TACTCGCCTA TCTCCTCCCA AATGATGCAC 
CATCCACAGT CCACCTTGTG GGGTTACAAC CTCGTGGGCC AGCCCCAGCA GCCCGGCTTT 
TTCCTTCAGA ACCAATCTCT TACTCCAGGT GGCTCCAGAT TGGACCCTGC AGGCTCCTTT 
GTCCCAACCA ACACCAAACA AGCTCTGTCA AACATGCTAC AGCGGCGCTC AGGCGCCATG 
ATGCAGCCGC CTTCTCTTCA TGCAATCACA TCGCAGCAGC AGTTGATACA GATGAAGCTT 
CTGCAGCAGC AGCAGCAACA GCGACTTCTC AGGCAAGCCC AGACTCGGCC TTTCCAACAG 
GGCCAGCCGG GGGACCAGGC TGCTCTCTTT GCTGCGCAAG CACGGCCCTC CCCTCAGCTC 
CCTCAGTATC CAGGGCTGCA GCAAGCACAG ACCATGCCAC AGGGCTATAC AATGTATGGG 
ACACAGATGC CTTTGCAGCA GACATCGCAG CAGCAGGCTG GCAGTGTGGT CCTGTCTCCC 
AGCTATAACT CCAGAGCCTA TCCGGCCGCA CATTCCAACC CCGTGCTAAT GGAAAGACTC 
AGACAGATTC AGCAGCAGCC GAGTGGCTAT GTTCAGCAGC AGGCCTCGCC GTACCTGCAG 
CCCCTGACTG GCTCTCAGAG ACTGAACCAT CAGGCTCTAC AGCAGAGCCC TCTGGTGGGC 
GGGGGAATTG ATGCTGTGCT GACTTCTGCA CATCCAAACC TTCCCTCCGT GCCCCTGCCT 
CAGGATCCCA TGAGACCCAG ACAGCCGCAA GTTCGACAGC AGCAGAGACT CCTCCAGATG 
CAGCAGCCCC AGCAGCCCCA GCCCCAGCAG CCTCCCCAGC CCCAGCAGTC CTCGCAGTCC 
CAGAGTCAGA CCCTTGGTCT CCAAGCAATG CAGCCCCAGC AGCCCTTGTT TCCCAGGCAA 
GGCTTGCAGC AGACCCAGCA GCAGCAGCAG ACGGCCGCCT TGGTGCGGCA GCTCCAGAAG 
CAGCTTTCCA GCAACCAGCC ACAGCAAGGA GTGACTCCGT ATGGGCATCC TTCACACTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713550.1
CDS	402..6944
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEEQSIMAHMGIDSGTTNIFDEVDKSDFKTDFGSEFPIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLPSVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_006713487.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713487.3

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAGT GTCAGCTCTT TAAAGAATGA TGACTTCACC 
ATGAGAGGTT TGCGATGTGA TGGGAATGCT GATGATATCT GGACTGCCTC ACAAAATCCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACTGCCAATT TAAGAGAATA CGCTAGATAT 
GTACTGAGGA CTATCTGTCA ACAGGAATGG GTAGGAGAGC ATTGCTTAAA AGAACCTGAA 
AGATTATGTA CAGACAAAGA ACTTATATTG GACCCTGTGC TTTCAAATAT GCAAGCACAG 
AAATTACTGC AGCTTATCTG TTATCCTCAT GGCATTAAAG AATGTACCGA GGGGGACAAT 
CTGCAAAGAC AGCACATTAA GCGTATTCTT CAGAATCTTG AGCAGTGGAC ACTGAGGCAA 
TCCTGGTTAG AACTCCAGCT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCT 
GTGGCCGAAA TGAACAACTT ACTGGACAAT ATTGCAAAGG CAACAATAGA GGTATTCCAG 
CAGTCTGCAG ACCTAAATAA TTCTTCTAAT TCTGGCATGA GCCTCTTCAA CCCAAACAGT 
ATTGGAAGTG CTGATACAAG TAGCACGAGA CAGAATGGAA TAAAGACATT CCTAAGTTCC 
TCCGAACGCA GGGGTGTATG GTTGGTGGCC CCCCTCATCG CCAGGTTGCC AACTTCTGTG 
CAAGGAAGAG TGCTGAAAGC CGCTGGGGAA GAGCTGGAGA AGGGACAGCA CTTGGGTTCT 
TCTTCCAAAA AGGAAAGGGA CAGACAGAAA CAGAAAAGTA TGTCTCTTTT GAGTCAACAA 
CCCTTCCTCT CACTGGTACT TACCTGCCTT AAGGGACAAG ATGAACAAAG GGAAGGCCTC 
CTAACATCTC TCCAGAATCA AGTTAACCAG ATTTTAAGTA ACTGGAGAGA AGAACGATAC 
CAAGATGACA TAAAAGCGCG GCAGATGATG CACGAAGCAT TGCAACTCCG CCTAAATTTG 
GTAGGAGGAA TGTTTGACAC GGTGCAGAGG AGCACCCAGT GGACTACAGA CTGGGCCCTG 
CTACTCCTTC AGATCATTAC TTCAGGAACT GTTGACATGC ACACTAACAA TGAATTATTC 
ACAACAGTTC TTGACATGCT GGGTGTTTTA ATCAATGGAA CGTTAGCCTC TGACCTATCA 
AATGCATCCC CTGGGGGATC TGAAGAGAAC AAGCGTGCAT ACATGAATTT AGTAAAGAAA 
CTGAAAAAAG AGCTAGGAGA CAAGCGATCA GAAAGTATTG ACAAAGTTCG ACAGTTACTA 
CCTTTGCCGA AACAGACATG TGATGTCATC ACTTGTGAAC CTATGGGTTC CTTGATTGAC 
ACAAAAGGAA ACAAAATTGC TGGATTTGAC TCTATAGATA AAAAACAGGG TCTCCAGGTC 
TCTACGAAGC AGAAGGTGTC CCCGTGGGAC TTGTTTGAGG GTCAGAAGAA CCCAGCTCCT 
TTGTCCTGGG CCTGGTTTGG GACAGTCCGA GTGGACCGAA GAGTGATCAA GTACGAGGAG 
CAGCATCACC TCCTGCTGTA TCACACACAC CCCATGCCCA AGCCCCGCAG TTACTACCTC 
CAGCCACTGC CCCTGCCTCC TGAGGAGGAA GAGGAAGAGC CCACATCTCC AGTTTCTCAG 
GAACCAGAAA GGAAGTCCGC TGAGCTGTCA GATCAGGGAA AAACCACAAC AGATGAAGAA 
AAGAAAACAA AAGGAAGGAA GCGCAAGACG AAATCTAGCT CAAGAGTTGA TGAATATCCA 
CAGAGCAACA TATACCGAGT GCCTCCTAAT TACTCGCCTA TCTCCTCCCA AATGATGCAC 
CATCCACAGT CCACCTTGTG GGGTTACAAC CTCGTGGGCC AGCCCCAGCA GCCCGGCTTT 
TTCCTTCAGA ACCAATCTCT TACTCCAGGT GGCTCCAGAT TGGACCCTGC AGGCTCCTTT 
GTCCCAACCA ACACCAAACA AGCTCTGTCA AACATGCTAC AGCGGCGCTC AGGCGCCATG 
ATGCAGCCGC CTTCTCTTCA TGCAATCACA TCGCAGCAGC AGTTGATACA GATGAAGCTT 
CTGCAGCAGC AGCAGCAACA GCGACTTCTC AGGCAAGCCC AGACTCGGCC TTTCCAACAG 
GGCCAGCCGG GGGACCAGGC TGCTCTCTTT GCTGCGCAAG CACGGCCCTC CCCTCAGCTC 
CCTCAGTATC CAGGGCTGCA GCAAGCACAG ACCATGCCAC AGGGCTATAC AATGTATGGG 
ACACAGATGC CTTTGCAGCA GACATCGCAG CAGCAGGCTG GCAGTGTGGT CCTGTCTCCC 
AGCTATAACT CCAGAGCCTA TCCGGCCGCA CATTCCAACC CCGTGCTAAT GGAAAGACTC 
AGACAGATTC AGCAGCAGCC GAGTGGCTAT GTTCAGCAGC AGGCCTCGCC GTACCTGCAG 
CCCCTGACTG GCTCTCAGAG ACTGAACCAT CAGGCTCTAC AGCAGAGCCC TCTGGTGGGC 
GGGGGAATTG ATGCTGTGCT GACTTCTGCA CATCCAAACC TTCCCTCCGT GCCCCTGCCT 
CAGGATCCCA TGAGACCCAG ACAGCCGCAA GTTCGACAGC AGCAGAGACT CCTCCAGATG 
CAGCAGCCCC AGCAGCCCCA GCCCCAGCAG CCTCCCCAGC CCCAGCAGTC CTCGCAGTCC 
CAGAGTCAGA CCCTTGGTCT CCAAGCAATG CAGCCCCAGC AGCCCTTGTT TCCCAGGCAA 
GGCTTGCAGC AGACCCAGCA GCAGCAGCAG ACGGCCGCCT TGGTGCGGCA GCTCCAGAAG 
CAGCTTTCCA GCAACCAGCC ACAGCAAGGA GTGACTCCGT ATGGGCATCC TTCACACTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_017005676.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861165.1 Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X1, mRNA.

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CloneID	OHu93784
Clone ID Related Accession (Same CDS sequence)	XM_017005676.1
Accession Version	XM_017005676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAGT GTCAGCTCTT TAAAGAATGA TGACTTCACC 
ATGAGAGGTT TGCGATGTGA TGGGAATGCT GATGATATCT GGACTGCCTC ACAAAATCCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACTGCCAATT TAAGAGAATA CGCTAGATAT 
GTACTGAGGA CTATCTGTCA ACAGGAATGG GTAGGAGAGC ATTGCTTAAA AGAACCTGAA 
AGATTATGTA CAGACAAAGA ACTTATATTG GACCCTGTGC TTTCAAATAT GCAAGCACAG 
AAATTACTGC AGCTTATCTG TTATCCTCAT GGCATTAAAG AATGTACCGA GGGGGACAAT 
CTGCAAAGAC AGCACATTAA GCGTATTCTT CAGAATCTTG AGCAGTGGAC ACTGAGGCAA 
TCCTGGTTAG AACTCCAGCT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCT 
GTGGCCGAAA TGAACAACTT ACTGGACAAT ATTGCAAAGG CAACAATAGA GGTATTCCAG 
CAGTCTGCAG ACCTAAATAA TTCTTCTAAT TCTGGCATGA GCCTCTTCAA CCCAAACAGT 
ATTGGAAGTG CTGATACAAG TAGCACGAGA CAGAATGGAA TAAAGACATT CCTAAGTTCC 
TCCGAACGCA GGGGTGTATG GTTGGTGGCC CCCCTCATCG CCAGGTTGCC AACTTCTGTG 
CAAGGAAGAG TGCTGAAAGC CGCTGGGGAA GAGCTGGAGA AGGGACAGCA CTTGGGTTCT 
TCTTCCAAAA AGGAAAGGGA CAGACAGAAA CAGAAAAGTA TGTCTCTTTT GAGTCAACAA 
CCCTTCCTCT CACTGGTACT TACCTGCCTT AAGGGACAAG ATGAACAAAG GGAAGGCCTC 
CTAACATCTC TCCAGAATCA AGTTAACCAG ATTTTAAGTA ACTGGAGAGA AGAACGATAC 
CAAGATGACA TAAAAGCGCG GCAGATGATG CACGAAGCAT TGCAACTCCG CCTAAATTTG 
GTAGGAGGAA TGTTTGACAC GGTGCAGAGG AGCACCCAGT GGACTACAGA CTGGGCCCTG 
CTACTCCTTC AGATCATTAC TTCAGGAACT GTTGACATGC ACACTAACAA TGAATTATTC 
ACAACAGTTC TTGACATGCT GGGTGTTTTA ATCAATGGAA CGTTAGCCTC TGACCTATCA 
AATGCATCCC CTGGGGGATC TGAAGAGAAC AAGCGTGCAT ACATGAATTT AGTAAAGAAA 
CTGAAAAAAG AGCTAGGAGA CAAGCGATCA GAAAGTATTG ACAAAGTTCG ACAGTTACTA 
CCTTTGCCGA AACAGACATG TGATGTCATC ACTTGTGAAC CTATGGGTTC CTTGATTGAC 
ACAAAAGGAA ACAAAATTGC TGGATTTGAC TCTATAGATA AAAAACAGGG TCTCCAGGTC 
TCTACGAAGC AGAAGGTGTC CCCGTGGGAC TTGTTTGAGG GTCAGAAGAA CCCAGCTCCT 
TTGTCCTGGG CCTGGTTTGG GACAGTCCGA GTGGACCGAA GAGTGATCAA GTACGAGGAG 
CAGCATCACC TCCTGCTGTA TCACACACAC CCCATGCCCA AGCCCCGCAG TTACTACCTC 
CAGCCACTGC CCCTGCCTCC TGAGGAGGAA GAGGAAGAGC CCACATCTCC AGTTTCTCAG 
GAACCAGAAA GGAAGTCCGC TGAGCTGTCA GATCAGGGAA AAACCACAAC AGATGAAGAA 
AAGAAAACAA AAGGAAGGAA GCGCAAGACG AAATCTAGCT CAAGAGTTGA TGAATATCCA 
CAGAGCAACA TATACCGAGT GCCTCCTAAT TACTCGCCTA TCTCCTCCCA AATGATGCAC 
CATCCACAGT CCACCTTGTG GGGTTACAAC CTCGTGGGCC AGCCCCAGCA GCCCGGCTTT 
TTCCTTCAGA ACCAATCTCT TACTCCAGGT GGCTCCAGAT TGGACCCTGC AGGCTCCTTT 
GTCCCAACCA ACACCAAACA AGCTCTGTCA AACATGCTAC AGCGGCGCTC AGGCGCCATG 
ATGCAGCCGC CTTCTCTTCA TGCAATCACA TCGCAGCAGC AGTTGATACA GATGAAGCTT 
CTGCAGCAGC AGCAGCAACA GCGACTTCTC AGGCAAGCCC AGACTCGGCC TTTCCAACAG 
GACTTCTGTA ACTCTGCTGT CTCCCTTCTG GAAGACTTTG ACAACATAAT GGGCCAGCCG 
GGGGACCAGG CTGCTCTCTT TGCTGCGCAA GCACGGCCCT CCCCTCAGCT CCCTCAGTAT 
CCAGGGCTGC AGCAAGCACA GACCATGCCA CAGGGCTATA CAATGTATGG GACACAGATG 
CCTTTGCAGC AGACATCGCA GCAGCAGGCT GGCAGTGTGG TCCTGTCTCC CAGCTATAAC 
TCCAGAGCCT ATCCGGCCGC ACATTCCAAC CCCGTGCTAA TGGAAAGACT CAGACAGATT 
CAGCAGCAGC CGAGTGGCTA TGTTCAGCAG CAGGCCTCGC CGTACCTGCA GCCCCTGACT 
GGCTCTCAGA GACTGAACCA TCAGGCTCTA CAGCAGAGCC CTCTGGTGGG CGGGGGAATT 
GATGCTGTGC TGACTTCTGC ACATCCAAAC CTTCCCTCCG TGCCCCTGCC TCAGGATCCC 
ATGAGACCCA GACAGCCGCA AGTTCGACAG CAGCAGAGAC TCCTCCAGAT GCAGCAGCCC 
CAGCAGCCCC AGCCCCAGCA GCCTCCCCAG CCCCAGCAGT CCTCGCAGTC CCAGAGTCAG 
ACCCTTGGTC TCCAAGCAAT GCAGCCCCAG CAGCCCTTGT TTCCCAGGCA AGGCTTGCAG 
CAGACCCAGC AGCAGCAGCA GACGGCCGCC TTGGTGCGGC AGCTCCAGAA GCAGCTTTCC 
AGCAACCAGC CACAGCAAGG AGTGACTCCG TATGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861165.1
CDS	402..6995
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEEQSIMAHMGIDSGTTNIFDEVDKSDFKTDFGSEFPIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQDFCNSAVSLLEDFDNIMGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLPSVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_017005676.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005676.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAGT GTCAGCTCTT TAAAGAATGA TGACTTCACC 
ATGAGAGGTT TGCGATGTGA TGGGAATGCT GATGATATCT GGACTGCCTC ACAAAATCCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACTGCCAATT TAAGAGAATA CGCTAGATAT 
GTACTGAGGA CTATCTGTCA ACAGGAATGG GTAGGAGAGC ATTGCTTAAA AGAACCTGAA 
AGATTATGTA CAGACAAAGA ACTTATATTG GACCCTGTGC TTTCAAATAT GCAAGCACAG 
AAATTACTGC AGCTTATCTG TTATCCTCAT GGCATTAAAG AATGTACCGA GGGGGACAAT 
CTGCAAAGAC AGCACATTAA GCGTATTCTT CAGAATCTTG AGCAGTGGAC ACTGAGGCAA 
TCCTGGTTAG AACTCCAGCT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCT 
GTGGCCGAAA TGAACAACTT ACTGGACAAT ATTGCAAAGG CAACAATAGA GGTATTCCAG 
CAGTCTGCAG ACCTAAATAA TTCTTCTAAT TCTGGCATGA GCCTCTTCAA CCCAAACAGT 
ATTGGAAGTG CTGATACAAG TAGCACGAGA CAGAATGGAA TAAAGACATT CCTAAGTTCC 
TCCGAACGCA GGGGTGTATG GTTGGTGGCC CCCCTCATCG CCAGGTTGCC AACTTCTGTG 
CAAGGAAGAG TGCTGAAAGC CGCTGGGGAA GAGCTGGAGA AGGGACAGCA CTTGGGTTCT 
TCTTCCAAAA AGGAAAGGGA CAGACAGAAA CAGAAAAGTA TGTCTCTTTT GAGTCAACAA 
CCCTTCCTCT CACTGGTACT TACCTGCCTT AAGGGACAAG ATGAACAAAG GGAAGGCCTC 
CTAACATCTC TCCAGAATCA AGTTAACCAG ATTTTAAGTA ACTGGAGAGA AGAACGATAC 
CAAGATGACA TAAAAGCGCG GCAGATGATG CACGAAGCAT TGCAACTCCG CCTAAATTTG 
GTAGGAGGAA TGTTTGACAC GGTGCAGAGG AGCACCCAGT GGACTACAGA CTGGGCCCTG 
CTACTCCTTC AGATCATTAC TTCAGGAACT GTTGACATGC ACACTAACAA TGAATTATTC 
ACAACAGTTC TTGACATGCT GGGTGTTTTA ATCAATGGAA CGTTAGCCTC TGACCTATCA 
AATGCATCCC CTGGGGGATC TGAAGAGAAC AAGCGTGCAT ACATGAATTT AGTAAAGAAA 
CTGAAAAAAG AGCTAGGAGA CAAGCGATCA GAAAGTATTG ACAAAGTTCG ACAGTTACTA 
CCTTTGCCGA AACAGACATG TGATGTCATC ACTTGTGAAC CTATGGGTTC CTTGATTGAC 
ACAAAAGGAA ACAAAATTGC TGGATTTGAC TCTATAGATA AAAAACAGGG TCTCCAGGTC 
TCTACGAAGC AGAAGGTGTC CCCGTGGGAC TTGTTTGAGG GTCAGAAGAA CCCAGCTCCT 
TTGTCCTGGG CCTGGTTTGG GACAGTCCGA GTGGACCGAA GAGTGATCAA GTACGAGGAG 
CAGCATCACC TCCTGCTGTA TCACACACAC CCCATGCCCA AGCCCCGCAG TTACTACCTC 
CAGCCACTGC CCCTGCCTCC TGAGGAGGAA GAGGAAGAGC CCACATCTCC AGTTTCTCAG 
GAACCAGAAA GGAAGTCCGC TGAGCTGTCA GATCAGGGAA AAACCACAAC AGATGAAGAA 
AAGAAAACAA AAGGAAGGAA GCGCAAGACG AAATCTAGCT CAAGAGTTGA TGAATATCCA 
CAGAGCAACA TATACCGAGT GCCTCCTAAT TACTCGCCTA TCTCCTCCCA AATGATGCAC 
CATCCACAGT CCACCTTGTG GGGTTACAAC CTCGTGGGCC AGCCCCAGCA GCCCGGCTTT 
TTCCTTCAGA ACCAATCTCT TACTCCAGGT GGCTCCAGAT TGGACCCTGC AGGCTCCTTT 
GTCCCAACCA ACACCAAACA AGCTCTGTCA AACATGCTAC AGCGGCGCTC AGGCGCCATG 
ATGCAGCCGC CTTCTCTTCA TGCAATCACA TCGCAGCAGC AGTTGATACA GATGAAGCTT 
CTGCAGCAGC AGCAGCAACA GCGACTTCTC AGGCAAGCCC AGACTCGGCC TTTCCAACAG 
GACTTCTGTA ACTCTGCTGT CTCCCTTCTG GAAGACTTTG ACAACATAAT GGGCCAGCCG 
GGGGACCAGG CTGCTCTCTT TGCTGCGCAA GCACGGCCCT CCCCTCAGCT CCCTCAGTAT 
CCAGGGCTGC AGCAAGCACA GACCATGCCA CAGGGCTATA CAATGTATGG GACACAGATG 
CCTTTGCAGC AGACATCGCA GCAGCAGGCT GGCAGTGTGG TCCTGTCTCC CAGCTATAAC 
TCCAGAGCCT ATCCGGCCGC ACATTCCAAC CCCGTGCTAA TGGAAAGACT CAGACAGATT 
CAGCAGCAGC CGAGTGGCTA TGTTCAGCAG CAGGCCTCGC CGTACCTGCA GCCCCTGACT 
GGCTCTCAGA GACTGAACCA TCAGGCTCTA CAGCAGAGCC CTCTGGTGGG CGGGGGAATT 
GATGCTGTGC TGACTTCTGC ACATCCAAAC CTTCCCTCCG TGCCCCTGCC TCAGGATCCC 
ATGAGACCCA GACAGCCGCA AGTTCGACAG CAGCAGAGAC TCCTCCAGAT GCAGCAGCCC 
CAGCAGCCCC AGCCCCAGCA GCCTCCCCAG CCCCAGCAGT CCTCGCAGTC CCAGAGTCAG 
ACCCTTGGTC TCCAAGCAAT GCAGCCCCAG CAGCCCTTGT TTCCCAGGCA AGGCTTGCAG 
CAGACCCAGC AGCAGCAGCA GACGGCCGCC TTGGTGCGGC AGCTCCAGAA GCAGCTTTCC 
AGCAACCAGC CACAGCAAGG AGTGACTCCG TATGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_017005677.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861166.1 Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X2, mRNA.

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CloneID	OHu93785
Clone ID Related Accession (Same CDS sequence)	XM_017005677.1
Accession Version	XM_017005677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTGC CTGTTCAAAA GTAAAGCAAA CCATATATAA TAACGTGATG 
CCTGCAAATT CGAACTTGCG ATGGGATCCA GACTTCATGA TGGATTTTAT TGAGAATCCC 
TCAGCCCGCA GCATCAACTA CTCAATGCTG GGCAAGATCC TCAGTGACAA TGCGGCCAAT 
CGCTACAGCT TTGTCTGCAA TACACTCATG AATGTATGTA TGGGCCATCA GGATGCTGGC 
AGGATTAACG ACATAGCCAA TTTCTCCTCT GAGCTTACGG CTTGCTGCAC TGTTCTTAGT 
TCAGAATGGC TGGGGGTTCT GAAGGCTCTT TGTTGTTCTT CAAATCACGT GTGGGGGTTT 
AATGATGTAC TTTGCACTGT AGATGTGAGT GACCTTTCAT TCCATGATTC ATTAGCTACT 
TTCATTGCTA TTCTGATAGC ACGACAGTGT TTTTCCCTGG AGGACGTCGT GCAGCATGTC 
GCACTTCCCT CTCTTCTAGC AGCAGCTTGT GGGGATGCGG ACGCCGAGCC TGGGGCGAGA 
ATGACATGCC GACTCTTGCT TCATCTCTTC CGAGCTCCCC AGGCCTGCTT CTTACCTCAA 
GCAACGGGCA AACCTTTCCC TGGAATAAGA TCATCTTGTG ATAGACACCT CTTAGCCGCT 
GCTCACAACA GCATTGAAGT GGGAGCCGTG TTTGCTGTCT TAAAAGCAAT TATGATGCTT 
GGAGATGCCA AAATTGGCAA TAACAGTGTC AGCTCTTTAA AGAATGATGA CTTCACCATG 
AGAGGTTTGC GATGTGATGG GAATGCTGAT GATATCTGGA CTGCCTCACA AAATCCAAAA 
TCCTGTGGGA AAAGCATTTC CATAGAAACT GCCAATTTAA GAGAATACGC TAGATATGTA 
CTGAGGACTA TCTGTCAACA GGAATGGGTA GGAGAGCATT GCTTAAAAGA ACCTGAAAGA 
TTATGTACAG ACAAAGAACT TATATTGGAC CCTGTGCTTT CAAATATGCA AGCACAGAAA 
TTACTGCAGC TTATCTGTTA TCCTCATGGC ATTAAAGAAT GTACCGAGGG GGACAATCTG 
CAAAGACAGC ACATTAAGCG TATTCTTCAG AATCTTGAGC AGTGGACACT GAGGCAATCC 
TGGTTAGAAC TCCAGCTAAT GATCAAACAG TGCTTGAAGG ACCCTGGCTC TGGTTCTGTG 
GCCGAAATGA ACAACTTACT GGACAATATT GCAAAGGCAA CAATAGAGGT ATTCCAGCAG 
TCTGCAGACC TAAATAATTC TTCTAATTCT GGCATGAGCC TCTTCAACCC AAACAGTATT 
GGAAGTGCTG ATACAAGTAG CACGAGACAG AATGGAATAA AGACATTCCT AAGTTCCTCC 
GAACGCAGGG GTGTATGGTT GGTGGCCCCC CTCATCGCCA GGTTGCCAAC TTCTGTGCAA 
GGAAGAGTGC TGAAAGCCGC TGGGGAAGAG CTGGAGAAGG GACAGCACTT GGGTTCTTCT 
TCCAAAAAGG AAAGGGACAG ACAGAAACAG AAAAGTATGT CTCTTTTGAG TCAACAACCC 
TTCCTCTCAC TGGTACTTAC CTGCCTTAAG GGACAAGATG AACAAAGGGA AGGCCTCCTA 
ACATCTCTCC AGAATCAAGT TAACCAGATT TTAAGTAACT GGAGAGAAGA ACGATACCAA 
GATGACATAA AAGCGCGGCA GATGATGCAC GAAGCATTGC AACTCCGCCT AAATTTGGTA 
GGAGGAATGT TTGACACGGT GCAGAGGAGC ACCCAGTGGA CTACAGACTG GGCCCTGCTA 
CTCCTTCAGA TCATTACTTC AGGAACTGTT GACATGCACA CTAACAATGA ATTATTCACA 
ACAGTTCTTG ACATGCTGGG TGTTTTAATC AATGGAACGT TAGCCTCTGA CCTATCAAAT 
GCATCCCCTG GGGGATCTGA AGAGAACAAG CGTGCATACA TGAATTTAGT AAAGAAACTG 
AAAAAAGAGC TAGGAGACAA GCGATCAGAA AGTATTGACA AAGTTCGACA GTTACTACCT 
TTGCCGAAAC AGACATGTGA TGTCATCACT TGTGAACCTA TGGGTTCCTT GATTGACACA 
AAAGGAAACA AAATTGCTGG ATTTGACTCT ATAGATAAAA AACAGGGTCT CCAGGTCTCT 
ACGAAGCAGA AGGTGTCCCC GTGGGACTTG TTTGAGGGTC AGAAGAACCC AGCTCCTTTG 
TCCTGGGCCT GGTTTGGGAC AGTCCGAGTG GACCGAAGAG TGATCAAGTA CGAGGAGCAG 
CATCACCTCC TGCTGTATCA CACACACCCC ATGCCCAAGC CCCGCAGTTA CTACCTCCAG 
CCACTGCCCC TGCCTCCTGA GGAGGAAGAG GAAGAGCCCA CATCTCCAGT TTCTCAGGAA 
CCAGAAAGGA AGTCCGCTGA GCTGTCAGAT CAGGGAAAAA CCACAACAGA TGAAGAAAAG 
AAAACAAAAG GAAGGAAGCG CAAGACGAAA TCTAGCTCAA GAGTTGATGA ATATCCACAG 
AGCAACATAT ACCGAGTGCC TCCTAATTAC TCGCCTATCT CCTCCCAAAT GATGCACCAT 
CCACAGTCCA CCTTGTGGGG TTACAACCTC GTGGGCCAGC CCCAGCAGCC CGGCTTTTTC 
CTTCAGAACC AATCTCTTAC TCCAGGTGGC TCCAGATTGG ACCCTGCAGG CTCCTTTGTC 
CCAACCAACA CCAAACAAGC TCTGTCAAAC ATGCTACAGC GGCGCTCAGG CGCCATGATG 
CAGCCGCCTT CTCTTCATGC AATCACATCG CAGCAGCAGT TGATACAGAT GAAGCTTCTG 
CAGCAGCAGC AGCAACAGCG ACTTCTCAGG CAAGCCCAGA CTCGGCCTTT CCAACAGGAC 
TTCTGTAACT CTGCTGTCTC CCTTCTGGAA GACTTTGACA ACATAATGGG CCAGCCGGGG 
GACCAGGCTG CTCTCTTTGC TGCGCAAGCA CGGCCCTCCC CTCAGCTCCC TCAGTATCCA 
GGGCTGCAGC AAGCACAGAC CATGCCACAG GGCTATACAA TGTATGGGAC ACAGATGCCT 
TTGCAGCAGA CATCGCAGCA GCAGGCTGGC AGTGTGGTCC TGTCTCCCAG CTATAACTCC 
AGAGCCTATC CGGCCGCACA TTCCAACCCC GTGCTAATGG AAAGACTCAG ACAGATTCAG 
CAGCAGCCGA GTGGCTATGT TCAGCAGCAG GCCTCGCCGT ACCTGCAGCC CCTGACTGGC 
TCTCAGAGAC TGAACCATCA GGCTCTACAG CAGAGCCCTC TGGTGGGCGG GGGAATTGAT 
GCTGTGCTGA CTTCTGCACA TCCAAACCTT CCCTCCGTGC CCCTGCCTCA GGATCCCATG 
AGACCCAGAC AGCCGCAAGT TCGACAGCAG CAGAGACTCC TCCAGATGCA GCAGCCCCAG 
CAGCCCCAGC CCCAGCAGCC TCCCCAGCCC CAGCAGTCCT CGCAGTCCCA GAGTCAGACC 
CTTGGTCTCC AAGCAATGCA GCCCCAGCAG CCCTTGTTTC CCAGGCAAGG CTTGCAGCAG 
ACCCAGCAGC AGCAGCAGAC GGCCGCCTTG GTGCGGCAGC TCCAGAAGCA GCTTTCCAGC 
AACCAGCCAC AGCAAGGAGT GACTCCGTAT GGGCATCCTT CACACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861166.1
CDS	402..6992
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEEQSIMAHMGIDSGTTNIFDEVDKSDFKTDFGSEFPIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQDFCNSAVSLLEDFDNIMGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLPSVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_017005677.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005677.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTGC CTGTTCAAAA GTAAAGCAAA CCATATATAA TAACGTGATG 
CCTGCAAATT CGAACTTGCG ATGGGATCCA GACTTCATGA TGGATTTTAT TGAGAATCCC 
TCAGCCCGCA GCATCAACTA CTCAATGCTG GGCAAGATCC TCAGTGACAA TGCGGCCAAT 
CGCTACAGCT TTGTCTGCAA TACACTCATG AATGTATGTA TGGGCCATCA GGATGCTGGC 
AGGATTAACG ACATAGCCAA TTTCTCCTCT GAGCTTACGG CTTGCTGCAC TGTTCTTAGT 
TCAGAATGGC TGGGGGTTCT GAAGGCTCTT TGTTGTTCTT CAAATCACGT GTGGGGGTTT 
AATGATGTAC TTTGCACTGT AGATGTGAGT GACCTTTCAT TCCATGATTC ATTAGCTACT 
TTCATTGCTA TTCTGATAGC ACGACAGTGT TTTTCCCTGG AGGACGTCGT GCAGCATGTC 
GCACTTCCCT CTCTTCTAGC AGCAGCTTGT GGGGATGCGG ACGCCGAGCC TGGGGCGAGA 
ATGACATGCC GACTCTTGCT TCATCTCTTC CGAGCTCCCC AGGCCTGCTT CTTACCTCAA 
GCAACGGGCA AACCTTTCCC TGGAATAAGA TCATCTTGTG ATAGACACCT CTTAGCCGCT 
GCTCACAACA GCATTGAAGT GGGAGCCGTG TTTGCTGTCT TAAAAGCAAT TATGATGCTT 
GGAGATGCCA AAATTGGCAA TAACAGTGTC AGCTCTTTAA AGAATGATGA CTTCACCATG 
AGAGGTTTGC GATGTGATGG GAATGCTGAT GATATCTGGA CTGCCTCACA AAATCCAAAA 
TCCTGTGGGA AAAGCATTTC CATAGAAACT GCCAATTTAA GAGAATACGC TAGATATGTA 
CTGAGGACTA TCTGTCAACA GGAATGGGTA GGAGAGCATT GCTTAAAAGA ACCTGAAAGA 
TTATGTACAG ACAAAGAACT TATATTGGAC CCTGTGCTTT CAAATATGCA AGCACAGAAA 
TTACTGCAGC TTATCTGTTA TCCTCATGGC ATTAAAGAAT GTACCGAGGG GGACAATCTG 
CAAAGACAGC ACATTAAGCG TATTCTTCAG AATCTTGAGC AGTGGACACT GAGGCAATCC 
TGGTTAGAAC TCCAGCTAAT GATCAAACAG TGCTTGAAGG ACCCTGGCTC TGGTTCTGTG 
GCCGAAATGA ACAACTTACT GGACAATATT GCAAAGGCAA CAATAGAGGT ATTCCAGCAG 
TCTGCAGACC TAAATAATTC TTCTAATTCT GGCATGAGCC TCTTCAACCC AAACAGTATT 
GGAAGTGCTG ATACAAGTAG CACGAGACAG AATGGAATAA AGACATTCCT AAGTTCCTCC 
GAACGCAGGG GTGTATGGTT GGTGGCCCCC CTCATCGCCA GGTTGCCAAC TTCTGTGCAA 
GGAAGAGTGC TGAAAGCCGC TGGGGAAGAG CTGGAGAAGG GACAGCACTT GGGTTCTTCT 
TCCAAAAAGG AAAGGGACAG ACAGAAACAG AAAAGTATGT CTCTTTTGAG TCAACAACCC 
TTCCTCTCAC TGGTACTTAC CTGCCTTAAG GGACAAGATG AACAAAGGGA AGGCCTCCTA 
ACATCTCTCC AGAATCAAGT TAACCAGATT TTAAGTAACT GGAGAGAAGA ACGATACCAA 
GATGACATAA AAGCGCGGCA GATGATGCAC GAAGCATTGC AACTCCGCCT AAATTTGGTA 
GGAGGAATGT TTGACACGGT GCAGAGGAGC ACCCAGTGGA CTACAGACTG GGCCCTGCTA 
CTCCTTCAGA TCATTACTTC AGGAACTGTT GACATGCACA CTAACAATGA ATTATTCACA 
ACAGTTCTTG ACATGCTGGG TGTTTTAATC AATGGAACGT TAGCCTCTGA CCTATCAAAT 
GCATCCCCTG GGGGATCTGA AGAGAACAAG CGTGCATACA TGAATTTAGT AAAGAAACTG 
AAAAAAGAGC TAGGAGACAA GCGATCAGAA AGTATTGACA AAGTTCGACA GTTACTACCT 
TTGCCGAAAC AGACATGTGA TGTCATCACT TGTGAACCTA TGGGTTCCTT GATTGACACA 
AAAGGAAACA AAATTGCTGG ATTTGACTCT ATAGATAAAA AACAGGGTCT CCAGGTCTCT 
ACGAAGCAGA AGGTGTCCCC GTGGGACTTG TTTGAGGGTC AGAAGAACCC AGCTCCTTTG 
TCCTGGGCCT GGTTTGGGAC AGTCCGAGTG GACCGAAGAG TGATCAAGTA CGAGGAGCAG 
CATCACCTCC TGCTGTATCA CACACACCCC ATGCCCAAGC CCCGCAGTTA CTACCTCCAG 
CCACTGCCCC TGCCTCCTGA GGAGGAAGAG GAAGAGCCCA CATCTCCAGT TTCTCAGGAA 
CCAGAAAGGA AGTCCGCTGA GCTGTCAGAT CAGGGAAAAA CCACAACAGA TGAAGAAAAG 
AAAACAAAAG GAAGGAAGCG CAAGACGAAA TCTAGCTCAA GAGTTGATGA ATATCCACAG 
AGCAACATAT ACCGAGTGCC TCCTAATTAC TCGCCTATCT CCTCCCAAAT GATGCACCAT 
CCACAGTCCA CCTTGTGGGG TTACAACCTC GTGGGCCAGC CCCAGCAGCC CGGCTTTTTC 
CTTCAGAACC AATCTCTTAC TCCAGGTGGC TCCAGATTGG ACCCTGCAGG CTCCTTTGTC 
CCAACCAACA CCAAACAAGC TCTGTCAAAC ATGCTACAGC GGCGCTCAGG CGCCATGATG 
CAGCCGCCTT CTCTTCATGC AATCACATCG CAGCAGCAGT TGATACAGAT GAAGCTTCTG 
CAGCAGCAGC AGCAACAGCG ACTTCTCAGG CAAGCCCAGA CTCGGCCTTT CCAACAGGAC 
TTCTGTAACT CTGCTGTCTC CCTTCTGGAA GACTTTGACA ACATAATGGG CCAGCCGGGG 
GACCAGGCTG CTCTCTTTGC TGCGCAAGCA CGGCCCTCCC CTCAGCTCCC TCAGTATCCA 
GGGCTGCAGC AAGCACAGAC CATGCCACAG GGCTATACAA TGTATGGGAC ACAGATGCCT 
TTGCAGCAGA CATCGCAGCA GCAGGCTGGC AGTGTGGTCC TGTCTCCCAG CTATAACTCC 
AGAGCCTATC CGGCCGCACA TTCCAACCCC GTGCTAATGG AAAGACTCAG ACAGATTCAG 
CAGCAGCCGA GTGGCTATGT TCAGCAGCAG GCCTCGCCGT ACCTGCAGCC CCTGACTGGC 
TCTCAGAGAC TGAACCATCA GGCTCTACAG CAGAGCCCTC TGGTGGGCGG GGGAATTGAT 
GCTGTGCTGA CTTCTGCACA TCCAAACCTT CCCTCCGTGC CCCTGCCTCA GGATCCCATG 
AGACCCAGAC AGCCGCAAGT TCGACAGCAG CAGAGACTCC TCCAGATGCA GCAGCCCCAG 
CAGCCCCAGC CCCAGCAGCC TCCCCAGCCC CAGCAGTCCT CGCAGTCCCA GAGTCAGACC 
CTTGGTCTCC AAGCAATGCA GCCCCAGCAG CCCTTGTTTC CCAGGCAAGG CTTGCAGCAG 
ACCCAGCAGC AGCAGCAGAC GGCCGCCTTG GTGCGGCAGC TCCAGAAGCA GCTTTCCAGC 
AACCAGCCAC AGCAAGGAGT GACTCCGTAT GGGCATCCTT CACACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_017005678.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861167.1 Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X4, mRNA.

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CloneID	OHu93786
Clone ID Related Accession (Same CDS sequence)	XM_017005678.1
Accession Version	XM_017005678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAGT GTCAGCTCTT TAAAGAATGA TGACTTCACC 
ATGAGAGGTT TGCGATGTGA TGGGAATGCT GATGATATCT GGACTGCCTC ACAAAATCCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACTGCCAATT TAAGAGAATA CGCTAGATAT 
GTACTGAGGA CTATCTGTCA ACAGGAATGG GTAGGAGAGC ATTGCTTAAA AGAACCTGAA 
AGATTATGTA CAGACAAAGA ACTTATATTG GACCCTGTGC TTTCAAATAT GCAAGCACAG 
AAATTACTGC AGCTTATCTG TTATCCTCAT GGCATTAAAG AATGTACCGA GGGGGACAAT 
CTGCAAAGAC AGCACATTAA GCGTATTCTT CAGAATCTTG AGCAGTGGAC ACTGAGGCAA 
TCCTGGTTAG AACTCCAGCT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCT 
GTGGCCGAAA TGAACAACTT ACTGGACAAT ATTGCAAAGG CAACAATAGA GGTATTCCAG 
CAGTCTGCAG ACCTAAATAA TTCTTCTAAT TCTGGCATGA GCCTCTTCAA CCCAAACAGT 
ATTGGAAGTG CTGATACAAG TAGCACGAGA CAGAATGGAA TAAAGACATT CCTAAGTTCC 
TCCGAACGCA GGGGTGTATG GTTGGTGGCC CCCCTCATCG CCAGGTTGCC AACTTCTGTG 
CAAGGAAGAG TGCTGAAAGC CGCTGGGGAA GAGCTGGAGA AGGGACAGCA CTTGGGTTCT 
TCTTCCAAAA AGGAAAGGGA CAGACAGAAA CAGAAAAGTA TGTCTCTTTT GAGTCAACAA 
CCCTTCCTCT CACTGGTACT TACCTGCCTT AAGGGACAAG ATGAACAAAG GGAAGGCCTC 
CTAACATCTC TCCAGAATCA AGTTAACCAG ATTTTAAGTA ACTGGAGAGA AGAACGATAC 
CAAGATGACA TAAAAGCGCG GCAGATGATG CACGAAGCAT TGCAACTCCG CCTAAATTTG 
GTAGGAGGAA TGTTTGACAC GGTGCAGAGG AGCACCCAGT GGACTACAGA CTGGGCCCTG 
CTACTCCTTC AGATCATTAC TTCAGGAACT GTTGACATGC ACACTAACAA TGAATTATTC 
ACAACAGTTC TTGACATGCT GGGTGTTTTA ATCAATGGAA CGTTAGCCTC TGACCTATCA 
AATGCATCCC CTGGGGGATC TGAAGAGAAC AAGCGTGCAT ACATGAATTT AGTAAAGAAA 
CTGAAAAAAG AGCTAGGAGA CAAGCGATCA GAAAGTATTG ACAAAGTTCG ACAGTTACTA 
CCTTTGCCGA AACAGACATG TGATGTCATC ACTTGTGAAC CTATGGGTTC CTTGATTGAC 
ACAAAAGGAA ACAAAATTGC TGGATTTGAC TCTATAGATA AAAAACAGGG TCTCCAGGTC 
TCTACGAAGC AGAAGGTGTC CCCGTGGGAC TTGTTTGAGG GTCAGAAGAA CCCAGCTCCT 
TTGTCCTGGG CCTGGTTTGG GACAGTCCGA GTGGACCGAA GAGTGATCAA GTACGAGGAG 
CAGCATCACC TCCTGCTGTA TCACACACAC CCCATGCCCA AGCCCCGCAG TTACTACCTC 
CAGCCACTGC CCCTGCCTCC TGAGGAGGAA GAGGAAGAGC CCACATCTCC AGTTTCTCAG 
GAACCAGAAA GGAAGTCCGC TGAGCTGTCA GATCAGGGAA AAACCACAAC AGATGAAGAA 
AAGAAAACAA AAGGAAGGAA GCGCAAGACG AAATCTAGCT CAAGAGTTGA TGAATATCCA 
CAGAGCAACA TATACCGAGT GCCTCCTAAT TACTCGCCTA TCTCCTCCCA AATGATGCAC 
CATCCACAGT CCACCTTGTG GGGTTACAAC CTCGTGGGCC AGCCCCAGCA GCCCGGCTTT 
TTCCTTCAGA ACCAATCTCT TACTCCAGGT GGCTCCAGAT TGGACCCTGC AGGCTCCTTT 
GTCCCAACCA ACACCAAACA AGCTCTGTCA AACATGCTAC AGCGGCGCTC AGGCGCCATG 
ATGCAGCCGC CTTCTCTTCA TGCAATCACA TCGCAGCAGC AGTTGATACA GATGAAGCTT 
CTGCAGCAGC AGCAGCAACA GCGACTTCTC AGGCAAGCCC AGACTCGGCC TTTCCAACAG 
ACCATGCCAC AGGGCTATAC AATGTATGGG ACACAGATGC CTTTGCAGCA GACATCGCAG 
CAGCAGGCTG GCAGTGTGGT CCTGTCTCCC AGCTATAACT CCAGAGCCTA TCCGGCCGCA 
CATTCCAACC CCGTGCTAAT GGAAAGACTC AGACAGATTC AGCAGCAGCC GAGTGGCTAT 
GTTCAGCAGC AGGCCTCGCC GTACCTGCAG CCCCTGACTG GCTCTCAGAG ACTGAACCAT 
CAGGCTCTAC AGCAGAGCCC TCTGGTGGGC GGGGGAATTG ATGCTGTGCT GACTTCTGCA 
CATCCAAACC TTCCCTCCGT GCCCCTGCCT CAGGATCCCA TGAGACCCAG ACAGCCGCAA 
GTTCGACAGC AGCAGAGACT CCTCCAGATG CAGCAGCCCC AGCAGCCCCA GCCCCAGCAG 
CCTCCCCAGC CCCAGCAGTC CTCGCAGTCC CAGAGTCAGA CCCTTGGTCT CCAAGCAATG 
CAGCCCCAGC AGCCCTTGTT TCCCAGGCAA GGCTTGCAGC AGACCCAGCA GCAGCAGCAG 
ACGGCCGCCT TGGTGCGGCA GCTCCAGAAG CAGCTTTCCA GCAACCAGCC ACAGCAAGGA 
GTGACTCCGT ATGGGCATCC TTCACACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861167.1
CDS	402..6854
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEEQSIMAHMGIDSGTTNIFDEVDKSDFKTDFGSEFPIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLPSVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_017005678.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005678.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA ACAGAGTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACC ACTAACATTT TTGATGAAGT AGACAAGAGT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCTATGCC TGGAGAATCC 
TGTGAGAATG CCAACACTTC GTTGGGCAGA AGAATGTCAG TTAATTGTGA GAAGTTGGTG 
AAGAGGGAAA AGCCAAGGGA ATTAATTTTT CCATCTAATT ATGACCTCCT TCGCCACTTA 
CAGTATGCAA CACATTTTCC TATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTCTATGG AGTCGGCAAA GAGCGTGATG AAGCAAGGCA TCAGCTGAAG 
AAGATTACCA AAGATATCCT GAAAATTCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGGGACGAAG GACAAAAAGC CAGGAAGAAC AAACAGGAGA CATTTCCAAC ACTGGAGACT 
GTGTTCACTA AACTCCAGCT CCTTTCATAT TTTGATCAAC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCGTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTGAA CATCAACGGA 
CTAATTGACT TCGCAATACA GTTACTAAAT GAACTGAGTG TTGTGGAAGC TGAACTGCTC 
CTAAAATCCT CCAGCCTGGC AGGAAGTTAT ACAACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GCTATCACAG TTGTCTAATC TTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT CAAGCATGTC GTAAACCCCT CAGAATGTTC TTCCCCTGAA 
AGATGCATTT TAGCCTACCT CTATGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAATAACGTG 
ATGCCTGCAA ATTCGAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCAGCCC GCAGCATCAA CTACTCAATG CTGGGCAAGA TCCTCAGTGA CAATGCGGCC 
AATCGCTACA GCTTTGTCTG CAATACACTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGCAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTTA CGGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA CGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTTTCCC TGGAGGACGT CGTGCAGCAT 
GTCGCACTTC CCTCTCTTCT AGCAGCAGCT TGTGGGGATG CGGACGCCGA GCCTGGGGCG 
AGAATGACAT GCCGACTCTT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTCTTACCT 
CAAGCAACGG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAGT GTCAGCTCTT TAAAGAATGA TGACTTCACC 
ATGAGAGGTT TGCGATGTGA TGGGAATGCT GATGATATCT GGACTGCCTC ACAAAATCCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACTGCCAATT TAAGAGAATA CGCTAGATAT 
GTACTGAGGA CTATCTGTCA ACAGGAATGG GTAGGAGAGC ATTGCTTAAA AGAACCTGAA 
AGATTATGTA CAGACAAAGA ACTTATATTG GACCCTGTGC TTTCAAATAT GCAAGCACAG 
AAATTACTGC AGCTTATCTG TTATCCTCAT GGCATTAAAG AATGTACCGA GGGGGACAAT 
CTGCAAAGAC AGCACATTAA GCGTATTCTT CAGAATCTTG AGCAGTGGAC ACTGAGGCAA 
TCCTGGTTAG AACTCCAGCT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCT 
GTGGCCGAAA TGAACAACTT ACTGGACAAT ATTGCAAAGG CAACAATAGA GGTATTCCAG 
CAGTCTGCAG ACCTAAATAA TTCTTCTAAT TCTGGCATGA GCCTCTTCAA CCCAAACAGT 
ATTGGAAGTG CTGATACAAG TAGCACGAGA CAGAATGGAA TAAAGACATT CCTAAGTTCC 
TCCGAACGCA GGGGTGTATG GTTGGTGGCC CCCCTCATCG CCAGGTTGCC AACTTCTGTG 
CAAGGAAGAG TGCTGAAAGC CGCTGGGGAA GAGCTGGAGA AGGGACAGCA CTTGGGTTCT 
TCTTCCAAAA AGGAAAGGGA CAGACAGAAA CAGAAAAGTA TGTCTCTTTT GAGTCAACAA 
CCCTTCCTCT CACTGGTACT TACCTGCCTT AAGGGACAAG ATGAACAAAG GGAAGGCCTC 
CTAACATCTC TCCAGAATCA AGTTAACCAG ATTTTAAGTA ACTGGAGAGA AGAACGATAC 
CAAGATGACA TAAAAGCGCG GCAGATGATG CACGAAGCAT TGCAACTCCG CCTAAATTTG 
GTAGGAGGAA TGTTTGACAC GGTGCAGAGG AGCACCCAGT GGACTACAGA CTGGGCCCTG 
CTACTCCTTC AGATCATTAC TTCAGGAACT GTTGACATGC ACACTAACAA TGAATTATTC 
ACAACAGTTC TTGACATGCT GGGTGTTTTA ATCAATGGAA CGTTAGCCTC TGACCTATCA 
AATGCATCCC CTGGGGGATC TGAAGAGAAC AAGCGTGCAT ACATGAATTT AGTAAAGAAA 
CTGAAAAAAG AGCTAGGAGA CAAGCGATCA GAAAGTATTG ACAAAGTTCG ACAGTTACTA 
CCTTTGCCGA AACAGACATG TGATGTCATC ACTTGTGAAC CTATGGGTTC CTTGATTGAC 
ACAAAAGGAA ACAAAATTGC TGGATTTGAC TCTATAGATA AAAAACAGGG TCTCCAGGTC 
TCTACGAAGC AGAAGGTGTC CCCGTGGGAC TTGTTTGAGG GTCAGAAGAA CCCAGCTCCT 
TTGTCCTGGG CCTGGTTTGG GACAGTCCGA GTGGACCGAA GAGTGATCAA GTACGAGGAG 
CAGCATCACC TCCTGCTGTA TCACACACAC CCCATGCCCA AGCCCCGCAG TTACTACCTC 
CAGCCACTGC CCCTGCCTCC TGAGGAGGAA GAGGAAGAGC CCACATCTCC AGTTTCTCAG 
GAACCAGAAA GGAAGTCCGC TGAGCTGTCA GATCAGGGAA AAACCACAAC AGATGAAGAA 
AAGAAAACAA AAGGAAGGAA GCGCAAGACG AAATCTAGCT CAAGAGTTGA TGAATATCCA 
CAGAGCAACA TATACCGAGT GCCTCCTAAT TACTCGCCTA TCTCCTCCCA AATGATGCAC 
CATCCACAGT CCACCTTGTG GGGTTACAAC CTCGTGGGCC AGCCCCAGCA GCCCGGCTTT 
TTCCTTCAGA ACCAATCTCT TACTCCAGGT GGCTCCAGAT TGGACCCTGC AGGCTCCTTT 
GTCCCAACCA ACACCAAACA AGCTCTGTCA AACATGCTAC AGCGGCGCTC AGGCGCCATG 
ATGCAGCCGC CTTCTCTTCA TGCAATCACA TCGCAGCAGC AGTTGATACA GATGAAGCTT 
CTGCAGCAGC AGCAGCAACA GCGACTTCTC AGGCAAGCCC AGACTCGGCC TTTCCAACAG 
ACCATGCCAC AGGGCTATAC AATGTATGGG ACACAGATGC CTTTGCAGCA GACATCGCAG 
CAGCAGGCTG GCAGTGTGGT CCTGTCTCCC AGCTATAACT CCAGAGCCTA TCCGGCCGCA 
CATTCCAACC CCGTGCTAAT GGAAAGACTC AGACAGATTC AGCAGCAGCC GAGTGGCTAT 
GTTCAGCAGC AGGCCTCGCC GTACCTGCAG CCCCTGACTG GCTCTCAGAG ACTGAACCAT 
CAGGCTCTAC AGCAGAGCCC TCTGGTGGGC GGGGGAATTG ATGCTGTGCT GACTTCTGCA 
CATCCAAACC TTCCCTCCGT GCCCCTGCCT CAGGATCCCA TGAGACCCAG ACAGCCGCAA 
GTTCGACAGC AGCAGAGACT CCTCCAGATG CAGCAGCCCC AGCAGCCCCA GCCCCAGCAG 
CCTCCCCAGC CCCAGCAGTC CTCGCAGTCC CAGAGTCAGA CCCTTGGTCT CCAAGCAATG 
CAGCCCCAGC AGCCCTTGTT TCCCAGGCAA GGCTTGCAGC AGACCCAGCA GCAGCAGCAG 
ACGGCCGCCT TGGTGCGGCA GCTCCAGAAG CAGCTTTCCA GCAACCAGCC ACAGCAAGGA 
GTGACTCCGT ATGGGCATCC TTCACACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_017005679.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861168.1 Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X6, mRNA.

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CloneID	OHu93787
Clone ID Related Accession (Same CDS sequence)	XM_017005679.1
Accession Version	XM_017005679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGACT 
GTCACTCTCT GTTGCCCAAG TGCCTTGGTG TGGAATTATT CCACAAATGA AAATAAGAGC 
GCAAACCCAG GCTCACCCCT GGATCTGCTG CAGGTGGCCC CGTCCAGCCT CCCCATGCCG 
GGTGGGAACA CGGCTTTCAA TCAGCAGGTT CGGGCAAGGA TTTATGAAGT AGAACAACAG 
ATAAAACAAA GAGGCCGTGC AGTGGAAGTT CGGTGGTCAT TTGACAAGTG CCAAGAATCC 
ACAGCAGGGG TGACTATTAG TCGGGTTTTG CACACGTTGG AAGTTTTGGA TCGTCACTGT 
TTTGACCGAA CTGATTCCAG CAATTCCATG GAGACACTTT ATCATAAGAT TTTCTGGGCA 
AACCAAAACA AAGATAACCA AGAGGTTGCG CCCAACGATG AAGCTGTGGT GACGCTGTTA 
TGTGAATGGG CCGTGAGCTG CAAACGGTCT GGCAAGCACA GGGCCATGGC TGTGGCAAAA 
CTCCTGGAGA AGAGGCAAGC AGAAATTGAG GCAGAGAGAT GTGGTGAATC AGAAGTCTTA 
GATGAGAAGG AGTCTATTTC TTCATCCTCT CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG 
AATGTGCTGT TAAGGTTTTT AGATACACAG GCCCCCTCTT TGTCGGACCC AAACAGTGAA 
TGTGAAAAGG TGGAATTTGT GAACCTGGTG CTGCTCTTCT GCGAGTTCAT CCGCCATGAT 
GTCTTCTCCC ATGACGCATA CATGTGTACC CTCATATCTC GAGGAGATTT GTCAGTCACT 
GCCTCAACTC GGCCGCGGTC ACCAGTAGGG GAAAATGCAG ATGAACACTA TTCAAAGGAC 
CATGATGTGA AAATGGAGGA ACAGAGTATT ATGGCGCATA TGGGCATTGA CTCAGGAACC 
ACTAACATTT TTGATGAAGT AGACAAGAGT GACTTTAAAA CTGACTTTGG TTCGGAATTT 
CCAATTTTTT CTCCTATGCC TGGAGAATCC TGTGAGAATG CCAACACTTC GTTGGGCAGA 
AGAATGTCAG TTAATTGTGA GAAGTTGGTG AAGAGGGAAA AGCCAAGGGA ATTAATTTTT 
CCATCTAATT ATGACCTCCT TCGCCACTTA CAGTATGCAA CACATTTTCC TATACCTCTG 
GATGAATCTT CAAGTCATGA ATGTAACCAG CGCACAATCC TTCTCTATGG AGTCGGCAAA 
GAGCGTGATG AAGCAAGGCA TCAGCTGAAG AAGATTACCA AAGATATCCT GAAAATTCTA 
AATAAGAAGA GCACCACAGA GACAGGGGTT GGGGACGAAG GACAAAAAGC CAGGAAGAAC 
AAACAGGAGA CATTTCCAAC ACTGGAGACT GTGTTCACTA AACTCCAGCT CCTTTCATAT 
TTTGATCAAC ATCAAGTGAC ATCTCAGATT TCTAACAATG TGCTAGAACA AATCACAAGC 
TTTGCGTCAG GAACATCCTA TCATCTCCCT TTGGCTCACC ACATTCAGCT CATCTTTGAT 
CTCATGGAGC CAGCACTGAA CATCAACGGA CTAATTGACT TCGCAATACA GTTACTAAAT 
GAACTGAGTG TTGTGGAAGC TGAACTGCTC CTAAAATCCT CCAGCCTGGC AGGAAGTTAT 
ACAACAGGAC TGTGTGTCTG CATCGTGGCT GTTCTCAGGC GCTATCACAG TTGTCTAATC 
TTGAATCCTG ATCAGACAGC CCAGGTGTTT GAAGGGTTGT GTGGTGTGGT CAAGCATGTC 
GTAAACCCCT CAGAATGTTC TTCCCCTGAA AGATGCATTT TAGCCTACCT CTATGATCTC 
TATGTGTCAT GTAGCCACCT CAGAAGTAAA TTTGGAGACC TCTTCAGTAG TGCCTGTTCA 
AAAGTAAAGC AAACCATATA TAATAACGTG ATGCCTGCAA ATTCGAACTT GCGATGGGAT 
CCAGACTTCA TGATGGATTT TATTGAGAAT CCCTCAGCCC GCAGCATCAA CTACTCAATG 
CTGGGCAAGA TCCTCAGTGA CAATGCGGCC AATCGCTACA GCTTTGTCTG CAATACACTC 
ATGAATGTAT GTATGGGCCA TCAGGATGCT GGCAGGATTA ACGACATAGC CAATTTCTCC 
TCTGAGCTTA CGGCTTGCTG CACTGTTCTT AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT 
CTTTGTTGTT CTTCAAATCA CGTGTGGGGG TTTAATGATG TACTTTGCAC TGTAGATGTG 
AGTGACCTTT CATTCCATGA TTCATTAGCT ACTTTCATTG CTATTCTGAT AGCACGACAG 
TGTTTTTCCC TGGAGGACGT CGTGCAGCAT GTCGCACTTC CCTCTCTTCT AGCAGCAGCT 
TGTGGGGATG CGGACGCCGA GCCTGGGGCG AGAATGACAT GCCGACTCTT GCTTCATCTC 
TTCCGAGCTC CCCAGGCCTG CTTCTTACCT CAAGCAACGG GCAAACCTTT CCCTGGAATA 
AGATCATCTT GTGATAGACA CCTCTTAGCC GCTGCTCACA ACAGCATTGA AGTGGGAGCC 
GTGTTTGCTG TCTTAAAAGC AATTATGATG CTTGGAGATG CCAAAATTGG CAATAACAGT 
GTCAGCTCTT TAAAGAATGA TGACTTCACC ATGAGAGGTT TGCGATGTGA TGGGAATGCT 
GATGATATCT GGACTGCCTC ACAAAATCCA AAATCCTGTG GGAAAAGCAT TTCCATAGAA 
ACTGCCAATT TAAGAGAATA CGCTAGATAT GTACTGAGGA CTATCTGTCA ACAGGAATGG 
GTAGGAGAGC ATTGCTTAAA AGAACCTGAA AGATTATGTA CAGACAAAGA ACTTATATTG 
GACCCTGTGC TTTCAAATAT GCAAGCACAG AAATTACTGC AGCTTATCTG TTATCCTCAT 
GGCATTAAAG AATGTACCGA GGGGGACAAT CTGCAAAGAC AGCACATTAA GCGTATTCTT 
CAGAATCTTG AGCAGTGGAC ACTGAGGCAA TCCTGGTTAG AACTCCAGCT AATGATCAAA 
CAGTGCTTGA AGGACCCTGG CTCTGGTTCT GTGGCCGAAA TGAACAACTT ACTGGACAAT 
ATTGCAAAGG CAACAATAGA GGTATTCCAG CAGTCTGCAG ACCTAAATAA TTCTTCTAAT 
TCTGGCATGA GCCTCTTCAA CCCAAACAGT ATTGGAAGTG CTGATACAAG TAGCACGAGA 
CAGAATGGAA TAAAGACATT CCTAAGTTCC TCCGAACGCA GGGGTGTATG GTTGGTGGCC 
CCCCTCATCG CCAGGTTGCC AACTTCTGTG CAAGGAAGAG TGCTGAAAGC CGCTGGGGAA 
GAGCTGGAGA AGGGACAGCA CTTGGGTTCT TCTTCCAAAA AGGAAAGGGA CAGACAGAAA 
CAGAAAAGTA TGTCTCTTTT GAGTCAACAA CCCTTCCTCT CACTGGTACT TACCTGCCTT 
AAGGGACAAG ATGAACAAAG GGAAGGCCTC CTAACATCTC TCCAGAATCA AGTTAACCAG 
ATTTTAAGTA ACTGGAGAGA AGAACGATAC CAAGATGACA TAAAAGCGCG GCAGATGATG 
CACGAAGCAT TGCAACTCCG CCTAAATTTG GTAGGAGGAA TGTTTGACAC GGTGCAGAGG 
AGCACCCAGT GGACTACAGA CTGGGCCCTG CTACTCCTTC AGATCATTAC TTCAGGAACT 
GTTGACATGC ACACTAACAA TGAATTATTC ACAACAGTTC TTGACATGCT GGGTGTTTTA 
ATCAATGGAA CGTTAGCCTC TGACCTATCA AATGCATCCC CTGGGGGATC TGAAGAGAAC 
AAGCGTGCAT ACATGAATTT AGTAAAGAAA CTGAAAAAAG AGCTAGGAGA CAAGCGATCA 
GAAAGTATTG ACAAAGTTCG ACAGTTACTA CCTTTGCCGA AACAGACATG TGATGTCATC 
ACTTGTGAAC CTATGGGTTC CTTGATTGAC ACAAAAGGAA ACAAAATTGC TGGATTTGAC 
TCTATAGATA AAAAACAGGG TCTCCAGGTC TCTACGAAGC AGAAGGTGTC CCCGTGGGAC 
TTGTTTGAGG GTCAGAAGAA CCCAGCTCCT TTGTCCTGGG CCTGGTTTGG GACAGTCCGA 
GTGGACCGAA GAGTGATCAA GTACGAGGAG CAGCATCACC TCCTGCTGTA TCACACACAC 
CCCATGCCCA AGCCCCGCAG TTACTACCTC CAGCCACTGC CCCTGCCTCC TGAGGAGGAA 
GAGGAAGAGC CCACATCTCC AGTTTCTCAG GAACCAGAAA GGAAGTCCGC TGAGCTGTCA 
GATCAGGGAA AAACCACAAC AGATGAAGAA AAGAAAACAA AAGGAAGGAA GCGCAAGACG 
AAATCTAGCT CAAGAGTTGA TGAATATCCA CAGAGCAACA TATACCGAGT GCCTCCTAAT 
TACTCGCCTA TCTCCTCCCA AATGATGCAC CATCCACAGT CCACCTTGTG GGGTTACAAC 
CTCGTGGGCC AGCCCCAGCA GCCCGGCTTT TTCCTTCAGA ACCAATCTCT TACTCCAGGT 
GGCTCCAGAT TGGACCCTGC AGGCTCCTTT GTCCCAACCA ACACCAAACA AGCTCTGTCA 
AACATGCTAC AGCGGCGCTC AGGCGCCATG ATGCAGCCGC CTTCTCTTCA TGCAATCACA 
TCGCAGCAGC AGTTGATACA GATGAAGCTT CTGCAGCAGC AGCAGCAACA GCGACTTCTC 
AGGCAAGCCC AGACTCGGCC TTTCCAACAG GACTTCTGTA ACTCTGCTGT CTCCCTTCTG 
GAAGACTTTG ACAACATAAT GGGCCAGCCG GGGGACCAGG CTGCTCTCTT TGCTGCGCAA 
GCACGGCCCT CCCCTCAGCT CCCTCAGTAT CCAGGGCTGC AGCAAGCACA GACCATGCCA 
CAGGGCTATA CAATGTATGG GACACAGATG CCTTTGCAGC AGACATCGCA GCAGCAGGCT 
GGCAGTGTGG TCCTGTCTCC CAGCTATAAC TCCAGAGCCT ATCCGGCCGC ACATTCCAAC 
CCCGTGCTAA TGGAAAGACT CAGACAGATT CAGCAGCAGC CGAGTGGCTA TGTTCAGCAG 
CAGGCCTCGC CGTACCTGCA GCCCCTGACT GGCTCTCAGA GACTGAACCA TCAGGCTCTA 
CAGCAGAGCC CTCTGGTGGG CGGGGGAATT GATGCTGTGC TGACTTCTGC ACATCCAAAC 
CTTCCCTCCG TGCCCCTGCC TCAGGATCCC ATGAGACCCA GACAGCCGCA AGTTCGACAG 
CAGCAGAGAC TCCTCCAGAT GCAGCAGCCC CAGCAGCCCC AGCCCCAGCA GCCTCCCCAG 
CCCCAGCAGT CCTCGCAGTC CCAGAGTCAG ACCCTTGGTC TCCAAGCAAT GCAGCCCCAG 
CAGCCCTTGT TTCCCAGGCA AGGCTTGCAG CAGACCCAGC AGCAGCAGCA GACGGCCGCC 
TTGGTGCGGC AGCTCCAGAA GCAGCTTTCC AGCAACCAGC CACAGCAAGG AGTGACTCCG 
TATGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861168.1
CDS	402..6725
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEEQSIMAHMGIDSGTTNIFDEVDKSDFKTDFGSEFPIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQDFCNSAVSLLEDFDNIMGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLPSVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_017005679.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005679.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGACT 
GTCACTCTCT GTTGCCCAAG TGCCTTGGTG TGGAATTATT CCACAAATGA AAATAAGAGC 
GCAAACCCAG GCTCACCCCT GGATCTGCTG CAGGTGGCCC CGTCCAGCCT CCCCATGCCG 
GGTGGGAACA CGGCTTTCAA TCAGCAGGTT CGGGCAAGGA TTTATGAAGT AGAACAACAG 
ATAAAACAAA GAGGCCGTGC AGTGGAAGTT CGGTGGTCAT TTGACAAGTG CCAAGAATCC 
ACAGCAGGGG TGACTATTAG TCGGGTTTTG CACACGTTGG AAGTTTTGGA TCGTCACTGT 
TTTGACCGAA CTGATTCCAG CAATTCCATG GAGACACTTT ATCATAAGAT TTTCTGGGCA 
AACCAAAACA AAGATAACCA AGAGGTTGCG CCCAACGATG AAGCTGTGGT GACGCTGTTA 
TGTGAATGGG CCGTGAGCTG CAAACGGTCT GGCAAGCACA GGGCCATGGC TGTGGCAAAA 
CTCCTGGAGA AGAGGCAAGC AGAAATTGAG GCAGAGAGAT GTGGTGAATC AGAAGTCTTA 
GATGAGAAGG AGTCTATTTC TTCATCCTCT CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG 
AATGTGCTGT TAAGGTTTTT AGATACACAG GCCCCCTCTT TGTCGGACCC AAACAGTGAA 
TGTGAAAAGG TGGAATTTGT GAACCTGGTG CTGCTCTTCT GCGAGTTCAT CCGCCATGAT 
GTCTTCTCCC ATGACGCATA CATGTGTACC CTCATATCTC GAGGAGATTT GTCAGTCACT 
GCCTCAACTC GGCCGCGGTC ACCAGTAGGG GAAAATGCAG ATGAACACTA TTCAAAGGAC 
CATGATGTGA AAATGGAGGA ACAGAGTATT ATGGCGCATA TGGGCATTGA CTCAGGAACC 
ACTAACATTT TTGATGAAGT AGACAAGAGT GACTTTAAAA CTGACTTTGG TTCGGAATTT 
CCAATTTTTT CTCCTATGCC TGGAGAATCC TGTGAGAATG CCAACACTTC GTTGGGCAGA 
AGAATGTCAG TTAATTGTGA GAAGTTGGTG AAGAGGGAAA AGCCAAGGGA ATTAATTTTT 
CCATCTAATT ATGACCTCCT TCGCCACTTA CAGTATGCAA CACATTTTCC TATACCTCTG 
GATGAATCTT CAAGTCATGA ATGTAACCAG CGCACAATCC TTCTCTATGG AGTCGGCAAA 
GAGCGTGATG AAGCAAGGCA TCAGCTGAAG AAGATTACCA AAGATATCCT GAAAATTCTA 
AATAAGAAGA GCACCACAGA GACAGGGGTT GGGGACGAAG GACAAAAAGC CAGGAAGAAC 
AAACAGGAGA CATTTCCAAC ACTGGAGACT GTGTTCACTA AACTCCAGCT CCTTTCATAT 
TTTGATCAAC ATCAAGTGAC ATCTCAGATT TCTAACAATG TGCTAGAACA AATCACAAGC 
TTTGCGTCAG GAACATCCTA TCATCTCCCT TTGGCTCACC ACATTCAGCT CATCTTTGAT 
CTCATGGAGC CAGCACTGAA CATCAACGGA CTAATTGACT TCGCAATACA GTTACTAAAT 
GAACTGAGTG TTGTGGAAGC TGAACTGCTC CTAAAATCCT CCAGCCTGGC AGGAAGTTAT 
ACAACAGGAC TGTGTGTCTG CATCGTGGCT GTTCTCAGGC GCTATCACAG TTGTCTAATC 
TTGAATCCTG ATCAGACAGC CCAGGTGTTT GAAGGGTTGT GTGGTGTGGT CAAGCATGTC 
GTAAACCCCT CAGAATGTTC TTCCCCTGAA AGATGCATTT TAGCCTACCT CTATGATCTC 
TATGTGTCAT GTAGCCACCT CAGAAGTAAA TTTGGAGACC TCTTCAGTAG TGCCTGTTCA 
AAAGTAAAGC AAACCATATA TAATAACGTG ATGCCTGCAA ATTCGAACTT GCGATGGGAT 
CCAGACTTCA TGATGGATTT TATTGAGAAT CCCTCAGCCC GCAGCATCAA CTACTCAATG 
CTGGGCAAGA TCCTCAGTGA CAATGCGGCC AATCGCTACA GCTTTGTCTG CAATACACTC 
ATGAATGTAT GTATGGGCCA TCAGGATGCT GGCAGGATTA ACGACATAGC CAATTTCTCC 
TCTGAGCTTA CGGCTTGCTG CACTGTTCTT AGTTCAGAAT GGCTGGGGGT TCTGAAGGCT 
CTTTGTTGTT CTTCAAATCA CGTGTGGGGG TTTAATGATG TACTTTGCAC TGTAGATGTG 
AGTGACCTTT CATTCCATGA TTCATTAGCT ACTTTCATTG CTATTCTGAT AGCACGACAG 
TGTTTTTCCC TGGAGGACGT CGTGCAGCAT GTCGCACTTC CCTCTCTTCT AGCAGCAGCT 
TGTGGGGATG CGGACGCCGA GCCTGGGGCG AGAATGACAT GCCGACTCTT GCTTCATCTC 
TTCCGAGCTC CCCAGGCCTG CTTCTTACCT CAAGCAACGG GCAAACCTTT CCCTGGAATA 
AGATCATCTT GTGATAGACA CCTCTTAGCC GCTGCTCACA ACAGCATTGA AGTGGGAGCC 
GTGTTTGCTG TCTTAAAAGC AATTATGATG CTTGGAGATG CCAAAATTGG CAATAACAGT 
GTCAGCTCTT TAAAGAATGA TGACTTCACC ATGAGAGGTT TGCGATGTGA TGGGAATGCT 
GATGATATCT GGACTGCCTC ACAAAATCCA AAATCCTGTG GGAAAAGCAT TTCCATAGAA 
ACTGCCAATT TAAGAGAATA CGCTAGATAT GTACTGAGGA CTATCTGTCA ACAGGAATGG 
GTAGGAGAGC ATTGCTTAAA AGAACCTGAA AGATTATGTA CAGACAAAGA ACTTATATTG 
GACCCTGTGC TTTCAAATAT GCAAGCACAG AAATTACTGC AGCTTATCTG TTATCCTCAT 
GGCATTAAAG AATGTACCGA GGGGGACAAT CTGCAAAGAC AGCACATTAA GCGTATTCTT 
CAGAATCTTG AGCAGTGGAC ACTGAGGCAA TCCTGGTTAG AACTCCAGCT AATGATCAAA 
CAGTGCTTGA AGGACCCTGG CTCTGGTTCT GTGGCCGAAA TGAACAACTT ACTGGACAAT 
ATTGCAAAGG CAACAATAGA GGTATTCCAG CAGTCTGCAG ACCTAAATAA TTCTTCTAAT 
TCTGGCATGA GCCTCTTCAA CCCAAACAGT ATTGGAAGTG CTGATACAAG TAGCACGAGA 
CAGAATGGAA TAAAGACATT CCTAAGTTCC TCCGAACGCA GGGGTGTATG GTTGGTGGCC 
CCCCTCATCG CCAGGTTGCC AACTTCTGTG CAAGGAAGAG TGCTGAAAGC CGCTGGGGAA 
GAGCTGGAGA AGGGACAGCA CTTGGGTTCT TCTTCCAAAA AGGAAAGGGA CAGACAGAAA 
CAGAAAAGTA TGTCTCTTTT GAGTCAACAA CCCTTCCTCT CACTGGTACT TACCTGCCTT 
AAGGGACAAG ATGAACAAAG GGAAGGCCTC CTAACATCTC TCCAGAATCA AGTTAACCAG 
ATTTTAAGTA ACTGGAGAGA AGAACGATAC CAAGATGACA TAAAAGCGCG GCAGATGATG 
CACGAAGCAT TGCAACTCCG CCTAAATTTG GTAGGAGGAA TGTTTGACAC GGTGCAGAGG 
AGCACCCAGT GGACTACAGA CTGGGCCCTG CTACTCCTTC AGATCATTAC TTCAGGAACT 
GTTGACATGC ACACTAACAA TGAATTATTC ACAACAGTTC TTGACATGCT GGGTGTTTTA 
ATCAATGGAA CGTTAGCCTC TGACCTATCA AATGCATCCC CTGGGGGATC TGAAGAGAAC 
AAGCGTGCAT ACATGAATTT AGTAAAGAAA CTGAAAAAAG AGCTAGGAGA CAAGCGATCA 
GAAAGTATTG ACAAAGTTCG ACAGTTACTA CCTTTGCCGA AACAGACATG TGATGTCATC 
ACTTGTGAAC CTATGGGTTC CTTGATTGAC ACAAAAGGAA ACAAAATTGC TGGATTTGAC 
TCTATAGATA AAAAACAGGG TCTCCAGGTC TCTACGAAGC AGAAGGTGTC CCCGTGGGAC 
TTGTTTGAGG GTCAGAAGAA CCCAGCTCCT TTGTCCTGGG CCTGGTTTGG GACAGTCCGA 
GTGGACCGAA GAGTGATCAA GTACGAGGAG CAGCATCACC TCCTGCTGTA TCACACACAC 
CCCATGCCCA AGCCCCGCAG TTACTACCTC CAGCCACTGC CCCTGCCTCC TGAGGAGGAA 
GAGGAAGAGC CCACATCTCC AGTTTCTCAG GAACCAGAAA GGAAGTCCGC TGAGCTGTCA 
GATCAGGGAA AAACCACAAC AGATGAAGAA AAGAAAACAA AAGGAAGGAA GCGCAAGACG 
AAATCTAGCT CAAGAGTTGA TGAATATCCA CAGAGCAACA TATACCGAGT GCCTCCTAAT 
TACTCGCCTA TCTCCTCCCA AATGATGCAC CATCCACAGT CCACCTTGTG GGGTTACAAC 
CTCGTGGGCC AGCCCCAGCA GCCCGGCTTT TTCCTTCAGA ACCAATCTCT TACTCCAGGT 
GGCTCCAGAT TGGACCCTGC AGGCTCCTTT GTCCCAACCA ACACCAAACA AGCTCTGTCA 
AACATGCTAC AGCGGCGCTC AGGCGCCATG ATGCAGCCGC CTTCTCTTCA TGCAATCACA 
TCGCAGCAGC AGTTGATACA GATGAAGCTT CTGCAGCAGC AGCAGCAACA GCGACTTCTC 
AGGCAAGCCC AGACTCGGCC TTTCCAACAG GACTTCTGTA ACTCTGCTGT CTCCCTTCTG 
GAAGACTTTG ACAACATAAT GGGCCAGCCG GGGGACCAGG CTGCTCTCTT TGCTGCGCAA 
GCACGGCCCT CCCCTCAGCT CCCTCAGTAT CCAGGGCTGC AGCAAGCACA GACCATGCCA 
CAGGGCTATA CAATGTATGG GACACAGATG CCTTTGCAGC AGACATCGCA GCAGCAGGCT 
GGCAGTGTGG TCCTGTCTCC CAGCTATAAC TCCAGAGCCT ATCCGGCCGC ACATTCCAAC 
CCCGTGCTAA TGGAAAGACT CAGACAGATT CAGCAGCAGC CGAGTGGCTA TGTTCAGCAG 
CAGGCCTCGC CGTACCTGCA GCCCCTGACT GGCTCTCAGA GACTGAACCA TCAGGCTCTA 
CAGCAGAGCC CTCTGGTGGG CGGGGGAATT GATGCTGTGC TGACTTCTGC ACATCCAAAC 
CTTCCCTCCG TGCCCCTGCC TCAGGATCCC ATGAGACCCA GACAGCCGCA AGTTCGACAG 
CAGCAGAGAC TCCTCCAGAT GCAGCAGCCC CAGCAGCCCC AGCCCCAGCA GCCTCCCCAG 
CCCCAGCAGT CCTCGCAGTC CCAGAGTCAG ACCCTTGGTC TCCAAGCAAT GCAGCCCCAG 
CAGCCCTTGT TTCCCAGGCA AGGCTTGCAG CAGACCCAGC AGCAGCAGCA GACGGCCGCC 
TTGGTGCGGC AGCTCCAGAA GCAGCTTTCC AGCAACCAGC CACAGCAAGG AGTGACTCCG 
TATGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_017005680.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861169.1 Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X7, mRNA.

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CloneID	OHu93788
Clone ID Related Accession (Same CDS sequence)	XM_017005680.1
Accession Version	XM_017005680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA TGAATCTTCA 
AGTCATGAAT GTAACCAGCG CACAATCCTT CTCTATGGAG TCGGCAAAGA GCGTGATGAA 
GCAAGGCATC AGCTGAAGAA GATTACCAAA GATATCCTGA AAATTCTAAA TAAGAAGAGC 
ACCACAGAGA CAGGGGTTGG GGACGAAGGA CAAAAAGCCA GGAAGAACAA ACAGGAGACA 
TTTCCAACAC TGGAGACTGT GTTCACTAAA CTCCAGCTCC TTTCATATTT TGATCAACAT 
CAAGTGACAT CTCAGATTTC TAACAATGTG CTAGAACAAA TCACAAGCTT TGCGTCAGGA 
ACATCCTATC ATCTCCCTTT GGCTCACCAC ATTCAGCTCA TCTTTGATCT CATGGAGCCA 
GCACTGAACA TCAACGGACT AATTGACTTC GCAATACAGT TACTAAATGA ACTGAGTGTT 
GTGGAAGCTG AACTGCTCCT AAAATCCTCC AGCCTGGCAG GAAGTTATAC AACAGGACTG 
TGTGTCTGCA TCGTGGCTGT TCTCAGGCGC TATCACAGTT GTCTAATCTT GAATCCTGAT 
CAGACAGCCC AGGTGTTTGA AGGGTTGTGT GGTGTGGTCA AGCATGTCGT AAACCCCTCA 
GAATGTTCTT CCCCTGAAAG ATGCATTTTA GCCTACCTCT ATGATCTCTA TGTGTCATGT 
AGCCACCTCA GAAGTAAATT TGGAGACCTC TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA 
ACCATATATA ATAACGTGAT GCCTGCAAAT TCGAACTTGC GATGGGATCC AGACTTCATG 
ATGGATTTTA TTGAGAATCC CTCAGCCCGC AGCATCAACT ACTCAATGCT GGGCAAGATC 
CTCAGTGACA ATGCGGCCAA TCGCTACAGC TTTGTCTGCA ATACACTCAT GAATGTATGT 
ATGGGCCATC AGGATGCTGG CAGGATTAAC GACATAGCCA ATTTCTCCTC TGAGCTTACG 
GCTTGCTGCA CTGTTCTTAG TTCAGAATGG CTGGGGGTTC TGAAGGCTCT TTGTTGTTCT 
TCAAATCACG TGTGGGGGTT TAATGATGTA CTTTGCACTG TAGATGTGAG TGACCTTTCA 
TTCCATGATT CATTAGCTAC TTTCATTGCT ATTCTGATAG CACGACAGTG TTTTTCCCTG 
GAGGACGTCG TGCAGCATGT CGCACTTCCC TCTCTTCTAG CAGCAGCTTG TGGGGATGCG 
GACGCCGAGC CTGGGGCGAG AATGACATGC CGACTCTTGC TTCATCTCTT CCGAGCTCCC 
CAGGCCTGCT TCTTACCTCA AGCAACGGGC AAACCTTTCC CTGGAATAAG ATCATCTTGT 
GATAGACACC TCTTAGCCGC TGCTCACAAC AGCATTGAAG TGGGAGCCGT GTTTGCTGTC 
TTAAAAGCAA TTATGATGCT TGGAGATGCC AAAATTGGCA ATAACAGTGT CAGCTCTTTA 
AAGAATGATG ACTTCACCAT GAGAGGTTTG CGATGTGATG GGAATGCTGA TGATATCTGG 
ACTGCCTCAC AAAATCCAAA ATCCTGTGGG AAAAGCATTT CCATAGAAAC TGCCAATTTA 
AGAGAATACG CTAGATATGT ACTGAGGACT ATCTGTCAAC AGGAATGGGT AGGAGAGCAT 
TGCTTAAAAG AACCTGAAAG ATTATGTACA GACAAAGAAC TTATATTGGA CCCTGTGCTT 
TCAAATATGC AAGCACAGAA ATTACTGCAG CTTATCTGTT ATCCTCATGG CATTAAAGAA 
TGTACCGAGG GGGACAATCT GCAAAGACAG CACATTAAGC GTATTCTTCA GAATCTTGAG 
CAGTGGACAC TGAGGCAATC CTGGTTAGAA CTCCAGCTAA TGATCAAACA GTGCTTGAAG 
GACCCTGGCT CTGGTTCTGT GGCCGAAATG AACAACTTAC TGGACAATAT TGCAAAGGCA 
ACAATAGAGG TATTCCAGCA GTCTGCAGAC CTAAATAATT CTTCTAATTC TGGCATGAGC 
CTCTTCAACC CAAACAGTAT TGGAAGTGCT GATACAAGTA GCACGAGACA GAATGGAATA 
AAGACATTCC TAAGTTCCTC CGAACGCAGG GGTGTATGGT TGGTGGCCCC CCTCATCGCC 
AGGTTGCCAA CTTCTGTGCA AGGAAGAGTG CTGAAAGCCG CTGGGGAAGA GCTGGAGAAG 
GGACAGCACT TGGGTTCTTC TTCCAAAAAG GAAAGGGACA GACAGAAACA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC CTTCCTCTCA CTGGTACTTA CCTGCCTTAA GGGACAAGAT 
GAACAAAGGG AAGGCCTCCT AACATCTCTC CAGAATCAAG TTAACCAGAT TTTAAGTAAC 
TGGAGAGAAG AACGATACCA AGATGACATA AAAGCGCGGC AGATGATGCA CGAAGCATTG 
CAACTCCGCC TAAATTTGGT AGGAGGAATG TTTGACACGG TGCAGAGGAG CACCCAGTGG 
ACTACAGACT GGGCCCTGCT ACTCCTTCAG ATCATTACTT CAGGAACTGT TGACATGCAC 
ACTAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTTTTAAT CAATGGAACG 
TTAGCCTCTG ACCTATCAAA TGCATCCCCT GGGGGATCTG AAGAGAACAA GCGTGCATAC 
ATGAATTTAG TAAAGAAACT GAAAAAAGAG CTAGGAGACA AGCGATCAGA AAGTATTGAC 
AAAGTTCGAC AGTTACTACC TTTGCCGAAA CAGACATGTG ATGTCATCAC TTGTGAACCT 
ATGGGTTCCT TGATTGACAC AAAAGGAAAC AAAATTGCTG GATTTGACTC TATAGATAAA 
AAACAGGGTC TCCAGGTCTC TACGAAGCAG AAGGTGTCCC CGTGGGACTT GTTTGAGGGT 
CAGAAGAACC CAGCTCCTTT GTCCTGGGCC TGGTTTGGGA CAGTCCGAGT GGACCGAAGA 
GTGATCAAGT ACGAGGAGCA GCATCACCTC CTGCTGTATC ACACACACCC CATGCCCAAG 
CCCCGCAGTT ACTACCTCCA GCCACTGCCC CTGCCTCCTG AGGAGGAAGA GGAAGAGCCC 
ACATCTCCAG TTTCTCAGGA ACCAGAAAGG AAGTCCGCTG AGCTGTCAGA TCAGGGAAAA 
ACCACAACAG ATGAAGAAAA GAAAACAAAA GGAAGGAAGC GCAAGACGAA ATCTAGCTCA 
AGAGTTGATG AATATCCACA GAGCAACATA TACCGAGTGC CTCCTAATTA CTCGCCTATC 
TCCTCCCAAA TGATGCACCA TCCACAGTCC ACCTTGTGGG GTTACAACCT CGTGGGCCAG 
CCCCAGCAGC CCGGCTTTTT CCTTCAGAAC CAATCTCTTA CTCCAGGTGG CTCCAGATTG 
GACCCTGCAG GCTCCTTTGT CCCAACCAAC ACCAAACAAG CTCTGTCAAA CATGCTACAG 
CGGCGCTCAG GCGCCATGAT GCAGCCGCCT TCTCTTCATG CAATCACATC GCAGCAGCAG 
TTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAACAGC GACTTCTCAG GCAAGCCCAG 
ACTCGGCCTT TCCAACAGGA CTTCTGTAAC TCTGCTGTCT CCCTTCTGGA AGACTTTGAC 
AACATAATGG GCCAGCCGGG GGACCAGGCT GCTCTCTTTG CTGCGCAAGC ACGGCCCTCC 
CCTCAGCTCC CTCAGTATCC AGGGCTGCAG CAAGCACAGA CCATGCCACA GGGCTATACA 
ATGTATGGGA CACAGATGCC TTTGCAGCAG ACATCGCAGC AGCAGGCTGG CAGTGTGGTC 
CTGTCTCCCA GCTATAACTC CAGAGCCTAT CCGGCCGCAC ATTCCAACCC CGTGCTAATG 
GAAAGACTCA GACAGATTCA GCAGCAGCCG AGTGGCTATG TTCAGCAGCA GGCCTCGCCG 
TACCTGCAGC CCCTGACTGG CTCTCAGAGA CTGAACCATC AGGCTCTACA GCAGAGCCCT 
CTGGTGGGCG GGGGAATTGA TGCTGTGCTG ACTTCTGCAC ATCCAAACCT TCCCTCCGTG 
CCCCTGCCTC AGGATCCCAT GAGACCCAGA CAGCCGCAAG TTCGACAGCA GCAGAGACTC 
CTCCAGATGC AGCAGCCCCA GCAGCCCCAG CCCCAGCAGC CTCCCCAGCC CCAGCAGTCC 
TCGCAGTCCC AGAGTCAGAC CCTTGGTCTC CAAGCAATGC AGCCCCAGCA GCCCTTGTTT 
CCCAGGCAAG GCTTGCAGCA GACCCAGCAG CAGCAGCAGA CGGCCGCCTT GGTGCGGCAG 
CTCCAGAAGC AGCTTTCCAG CAACCAGCCA CAGCAAGGAG TGACTCCGTA TGGGCATCCT 
TCACACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861169.1
CDS	402..6713
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQDFCNSAVSLLEDFDNIMGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLPSVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_017005680.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005680.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAGCGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCACA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTAAAGCAAG GCTTCAATAA TCAGCCAGCC TTCACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTGAAGCTTA ACACTTTCCA GGACACGGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT TTCTGACTTA 
GCAGGAAATA AGCCACTTTC TATTTTGGCA AAAAAGGTTC CTATCCTTAG TAAAAAAGAG 
GATGTTTTTG CATATTTAGC TAAATATTCT GTGCCAATGG TTCGAGCAAC GTGGCTGATC 
AAGATGACTT GTGCCTATTA TTCTGCTATA TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
CCTGATCCGA ATTTGGAGTG GACACAGATA TCTACCAGAT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCCTCC AGCACGGGCG ATGGCCCTGT CCCTGTGCCA 
CCAGAGGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGCTAGC ATTTCACATG 
TTCCAGGAAG GAATGTTAGA AAAACACGAA TATTTGACAT GGATCCTGGA TGTTTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTGC TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTTCAGTC GGCCTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTTCCT TGCTGCTGAG CGATAGCCCC AACCTCCTTG CTGCCCACTC ACCCCACATG 
ATGATAGGAC CAAACAACTC GAGTATCGGG GCCCCCAGCC CTGGCCCCCC CGGCCCTGGC 
ATGAGCCCCG TGCAGCTGGC CTTCTCAGAT TTTCTTTCCT GTGCACAGCA TGGTCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAATGA AAATAAGAGC GCAAACCCAG GCTCACCCCT GGATCTGCTG 
CAGGTGGCCC CGTCCAGCCT CCCCATGCCG GGTGGGAACA CGGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAAGT AGAACAACAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCAT TTGACAAGTG CCAAGAATCC ACAGCAGGGG TGACTATTAG TCGGGTTTTG 
CACACGTTGG AAGTTTTGGA TCGTCACTGT TTTGACCGAA CTGATTCCAG CAATTCCATG 
GAGACACTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTTGCG 
CCCAACGATG AAGCTGTGGT GACGCTGTTA TGTGAATGGG CCGTGAGCTG CAAACGGTCT 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCCTGGAGA AGAGGCAAGC AGAAATTGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCTATTTC TTCATCCTCT 
CTTGCTGGAT CCAGTTTGCC TGTTTTCCAG AATGTGCTGT TAAGGTTTTT AGATACACAG 
GCCCCCTCTT TGTCGGACCC AAACAGTGAA TGTGAAAAGG TGGAATTTGT GAACCTGGTG 
CTGCTCTTCT GCGAGTTCAT CCGCCATGAT GTCTTCTCCC ATGACGCATA CATGTGTACC 
CTCATATCTC GAGGAGATTT GTCAGTCACT GCCTCAACTC GGCCGCGGTC ACCAGTAGGG 
GAAAATGCAG ATGAACACTA TTCAAAGGAC CATGATGTGA AAATGGAGGA TGAATCTTCA 
AGTCATGAAT GTAACCAGCG CACAATCCTT CTCTATGGAG TCGGCAAAGA GCGTGATGAA 
GCAAGGCATC AGCTGAAGAA GATTACCAAA GATATCCTGA AAATTCTAAA TAAGAAGAGC 
ACCACAGAGA CAGGGGTTGG GGACGAAGGA CAAAAAGCCA GGAAGAACAA ACAGGAGACA 
TTTCCAACAC TGGAGACTGT GTTCACTAAA CTCCAGCTCC TTTCATATTT TGATCAACAT 
CAAGTGACAT CTCAGATTTC TAACAATGTG CTAGAACAAA TCACAAGCTT TGCGTCAGGA 
ACATCCTATC ATCTCCCTTT GGCTCACCAC ATTCAGCTCA TCTTTGATCT CATGGAGCCA 
GCACTGAACA TCAACGGACT AATTGACTTC GCAATACAGT TACTAAATGA ACTGAGTGTT 
GTGGAAGCTG AACTGCTCCT AAAATCCTCC AGCCTGGCAG GAAGTTATAC AACAGGACTG 
TGTGTCTGCA TCGTGGCTGT TCTCAGGCGC TATCACAGTT GTCTAATCTT GAATCCTGAT 
CAGACAGCCC AGGTGTTTGA AGGGTTGTGT GGTGTGGTCA AGCATGTCGT AAACCCCTCA 
GAATGTTCTT CCCCTGAAAG ATGCATTTTA GCCTACCTCT ATGATCTCTA TGTGTCATGT 
AGCCACCTCA GAAGTAAATT TGGAGACCTC TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA 
ACCATATATA ATAACGTGAT GCCTGCAAAT TCGAACTTGC GATGGGATCC AGACTTCATG 
ATGGATTTTA TTGAGAATCC CTCAGCCCGC AGCATCAACT ACTCAATGCT GGGCAAGATC 
CTCAGTGACA ATGCGGCCAA TCGCTACAGC TTTGTCTGCA ATACACTCAT GAATGTATGT 
ATGGGCCATC AGGATGCTGG CAGGATTAAC GACATAGCCA ATTTCTCCTC TGAGCTTACG 
GCTTGCTGCA CTGTTCTTAG TTCAGAATGG CTGGGGGTTC TGAAGGCTCT TTGTTGTTCT 
TCAAATCACG TGTGGGGGTT TAATGATGTA CTTTGCACTG TAGATGTGAG TGACCTTTCA 
TTCCATGATT CATTAGCTAC TTTCATTGCT ATTCTGATAG CACGACAGTG TTTTTCCCTG 
GAGGACGTCG TGCAGCATGT CGCACTTCCC TCTCTTCTAG CAGCAGCTTG TGGGGATGCG 
GACGCCGAGC CTGGGGCGAG AATGACATGC CGACTCTTGC TTCATCTCTT CCGAGCTCCC 
CAGGCCTGCT TCTTACCTCA AGCAACGGGC AAACCTTTCC CTGGAATAAG ATCATCTTGT 
GATAGACACC TCTTAGCCGC TGCTCACAAC AGCATTGAAG TGGGAGCCGT GTTTGCTGTC 
TTAAAAGCAA TTATGATGCT TGGAGATGCC AAAATTGGCA ATAACAGTGT CAGCTCTTTA 
AAGAATGATG ACTTCACCAT GAGAGGTTTG CGATGTGATG GGAATGCTGA TGATATCTGG 
ACTGCCTCAC AAAATCCAAA ATCCTGTGGG AAAAGCATTT CCATAGAAAC TGCCAATTTA 
AGAGAATACG CTAGATATGT ACTGAGGACT ATCTGTCAAC AGGAATGGGT AGGAGAGCAT 
TGCTTAAAAG AACCTGAAAG ATTATGTACA GACAAAGAAC TTATATTGGA CCCTGTGCTT 
TCAAATATGC AAGCACAGAA ATTACTGCAG CTTATCTGTT ATCCTCATGG CATTAAAGAA 
TGTACCGAGG GGGACAATCT GCAAAGACAG CACATTAAGC GTATTCTTCA GAATCTTGAG 
CAGTGGACAC TGAGGCAATC CTGGTTAGAA CTCCAGCTAA TGATCAAACA GTGCTTGAAG 
GACCCTGGCT CTGGTTCTGT GGCCGAAATG AACAACTTAC TGGACAATAT TGCAAAGGCA 
ACAATAGAGG TATTCCAGCA GTCTGCAGAC CTAAATAATT CTTCTAATTC TGGCATGAGC 
CTCTTCAACC CAAACAGTAT TGGAAGTGCT GATACAAGTA GCACGAGACA GAATGGAATA 
AAGACATTCC TAAGTTCCTC CGAACGCAGG GGTGTATGGT TGGTGGCCCC CCTCATCGCC 
AGGTTGCCAA CTTCTGTGCA AGGAAGAGTG CTGAAAGCCG CTGGGGAAGA GCTGGAGAAG 
GGACAGCACT TGGGTTCTTC TTCCAAAAAG GAAAGGGACA GACAGAAACA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC CTTCCTCTCA CTGGTACTTA CCTGCCTTAA GGGACAAGAT 
GAACAAAGGG AAGGCCTCCT AACATCTCTC CAGAATCAAG TTAACCAGAT TTTAAGTAAC 
TGGAGAGAAG AACGATACCA AGATGACATA AAAGCGCGGC AGATGATGCA CGAAGCATTG 
CAACTCCGCC TAAATTTGGT AGGAGGAATG TTTGACACGG TGCAGAGGAG CACCCAGTGG 
ACTACAGACT GGGCCCTGCT ACTCCTTCAG ATCATTACTT CAGGAACTGT TGACATGCAC 
ACTAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTTTTAAT CAATGGAACG 
TTAGCCTCTG ACCTATCAAA TGCATCCCCT GGGGGATCTG AAGAGAACAA GCGTGCATAC 
ATGAATTTAG TAAAGAAACT GAAAAAAGAG CTAGGAGACA AGCGATCAGA AAGTATTGAC 
AAAGTTCGAC AGTTACTACC TTTGCCGAAA CAGACATGTG ATGTCATCAC TTGTGAACCT 
ATGGGTTCCT TGATTGACAC AAAAGGAAAC AAAATTGCTG GATTTGACTC TATAGATAAA 
AAACAGGGTC TCCAGGTCTC TACGAAGCAG AAGGTGTCCC CGTGGGACTT GTTTGAGGGT 
CAGAAGAACC CAGCTCCTTT GTCCTGGGCC TGGTTTGGGA CAGTCCGAGT GGACCGAAGA 
GTGATCAAGT ACGAGGAGCA GCATCACCTC CTGCTGTATC ACACACACCC CATGCCCAAG 
CCCCGCAGTT ACTACCTCCA GCCACTGCCC CTGCCTCCTG AGGAGGAAGA GGAAGAGCCC 
ACATCTCCAG TTTCTCAGGA ACCAGAAAGG AAGTCCGCTG AGCTGTCAGA TCAGGGAAAA 
ACCACAACAG ATGAAGAAAA GAAAACAAAA GGAAGGAAGC GCAAGACGAA ATCTAGCTCA 
AGAGTTGATG AATATCCACA GAGCAACATA TACCGAGTGC CTCCTAATTA CTCGCCTATC 
TCCTCCCAAA TGATGCACCA TCCACAGTCC ACCTTGTGGG GTTACAACCT CGTGGGCCAG 
CCCCAGCAGC CCGGCTTTTT CCTTCAGAAC CAATCTCTTA CTCCAGGTGG CTCCAGATTG 
GACCCTGCAG GCTCCTTTGT CCCAACCAAC ACCAAACAAG CTCTGTCAAA CATGCTACAG 
CGGCGCTCAG GCGCCATGAT GCAGCCGCCT TCTCTTCATG CAATCACATC GCAGCAGCAG 
TTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAACAGC GACTTCTCAG GCAAGCCCAG 
ACTCGGCCTT TCCAACAGGA CTTCTGTAAC TCTGCTGTCT CCCTTCTGGA AGACTTTGAC 
AACATAATGG GCCAGCCGGG GGACCAGGCT GCTCTCTTTG CTGCGCAAGC ACGGCCCTCC 
CCTCAGCTCC CTCAGTATCC AGGGCTGCAG CAAGCACAGA CCATGCCACA GGGCTATACA 
ATGTATGGGA CACAGATGCC TTTGCAGCAG ACATCGCAGC AGCAGGCTGG CAGTGTGGTC 
CTGTCTCCCA GCTATAACTC CAGAGCCTAT CCGGCCGCAC ATTCCAACCC CGTGCTAATG 
GAAAGACTCA GACAGATTCA GCAGCAGCCG AGTGGCTATG TTCAGCAGCA GGCCTCGCCG 
TACCTGCAGC CCCTGACTGG CTCTCAGAGA CTGAACCATC AGGCTCTACA GCAGAGCCCT 
CTGGTGGGCG GGGGAATTGA TGCTGTGCTG ACTTCTGCAC ATCCAAACCT TCCCTCCGTG 
CCCCTGCCTC AGGATCCCAT GAGACCCAGA CAGCCGCAAG TTCGACAGCA GCAGAGACTC 
CTCCAGATGC AGCAGCCCCA GCAGCCCCAG CCCCAGCAGC CTCCCCAGCC CCAGCAGTCC 
TCGCAGTCCC AGAGTCAGAC CCTTGGTCTC CAAGCAATGC AGCCCCAGCA GCCCTTGTTT 
CCCAGGCAAG GCTTGCAGCA GACCCAGCAG CAGCAGCAGA CGGCCGCCTT GGTGCGGCAG 
CTCCAGAAGC AGCTTTCCAG CAACCAGCCA CAGCAAGGAG TGACTCCGTA TGGGCATCCT 
TCACACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_017005681.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861170.1 Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X10, mRNA.

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CloneID	OHu93789
Clone ID Related Accession (Same CDS sequence)	XM_017005681.1
Accession Version	XM_017005681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAG CACTGAACAT CAACGGACTA ATTGACTTCG CAATACAGTT ACTAAATGAA 
CTGAGTGTTG TGGAAGCTGA ACTGCTCCTA AAATCCTCCA GCCTGGCAGG AAGTTATACA 
ACAGGACTGT GTGTCTGCAT CGTGGCTGTT CTCAGGCGCT ATCACAGTTG TCTAATCTTG 
AATCCTGATC AGACAGCCCA GGTGTTTGAA GGGTTGTGTG GTGTGGTCAA GCATGTCGTA 
AACCCCTCAG AATGTTCTTC CCCTGAAAGA TGCATTTTAG CCTACCTCTA TGATCTCTAT 
GTGTCATGTA GCCACCTCAG AAGTAAATTT GGAGACCTCT TCAGTAGTGC CTGTTCAAAA 
GTAAAGCAAA CCATATATAA TAACGTGATG CCTGCAAATT CGAACTTGCG ATGGGATCCA 
GACTTCATGA TGGATTTTAT TGAGAATCCC TCAGCCCGCA GCATCAACTA CTCAATGCTG 
GGCAAGATCC TCAGTGACAA TGCGGCCAAT CGCTACAGCT TTGTCTGCAA TACACTCATG 
AATGTATGTA TGGGCCATCA GGATGCTGGC AGGATTAACG ACATAGCCAA TTTCTCCTCT 
GAGCTTACGG CTTGCTGCAC TGTTCTTAGT TCAGAATGGC TGGGGGTTCT GAAGGCTCTT 
TGTTGTTCTT CAAATCACGT GTGGGGGTTT AATGATGTAC TTTGCACTGT AGATGTGAGT 
GACCTTTCAT TCCATGATTC ATTAGCTACT TTCATTGCTA TTCTGATAGC ACGACAGTGT 
TTTTCCCTGG AGGACGTCGT GCAGCATGTC GCACTTCCCT CTCTTCTAGC AGCAGCTTGT 
GGGGATGCGG ACGCCGAGCC TGGGGCGAGA ATGACATGCC GACTCTTGCT TCATCTCTTC 
CGAGCTCCCC AGGCCTGCTT CTTACCTCAA GCAACGGGCA AACCTTTCCC TGGAATAAGA 
TCATCTTGTG ATAGACACCT CTTAGCCGCT GCTCACAACA GCATTGAAGT GGGAGCCGTG 
TTTGCTGTCT TAAAAGCAAT TATGATGCTT GGAGATGCCA AAATTGGCAA TAACAGTGTC 
AGCTCTTTAA AGAATGATGA CTTCACCATG AGAGGTTTGC GATGTGATGG GAATGCTGAT 
GATATCTGGA CTGCCTCACA AAATCCAAAA TCCTGTGGGA AAAGCATTTC CATAGAAACT 
GCCAATTTAA GAGAATACGC TAGATATGTA CTGAGGACTA TCTGTCAACA GGAATGGGTA 
GGAGAGCATT GCTTAAAAGA ACCTGAAAGA TTATGTACAG ACAAAGAACT TATATTGGAC 
CCTGTGCTTT CAAATATGCA AGCACAGAAA TTACTGCAGC TTATCTGTTA TCCTCATGGC 
ATTAAAGAAT GTACCGAGGG GGACAATCTG CAAAGACAGC ACATTAAGCG TATTCTTCAG 
AATCTTGAGC AGTGGACACT GAGGCAATCC TGGTTAGAAC TCCAGCTAAT GATCAAACAG 
TGCTTGAAGG ACCCTGGCTC TGGTTCTGTG GCCGAAATGA ACAACTTACT GGACAATATT 
GCAAAGGCAA CAATAGAGGT ATTCCAGCAG TCTGCAGACC TAAATAATTC TTCTAATTCT 
GGCATGAGCC TCTTCAACCC AAACAGTATT GGAAGTGCTG ATACAAGTAG CACGAGACAG 
AATGGAATAA AGACATTCCT AAGTTCCTCC GAACGCAGGG GTGTATGGTT GGTGGCCCCC 
CTCATCGCCA GGTTGCCAAC TTCTGTGCAA GGAAGAGTGC TGAAAGCCGC TGGGGAAGAG 
CTGGAGAAGG GACAGCACTT GGGTTCTTCT TCCAAAAAGG AAAGGGACAG ACAGAAACAG 
AAAAGTATGT CTCTTTTGAG TCAACAACCC TTCCTCTCAC TGGTACTTAC CTGCCTTAAG 
GGACAAGATG AACAAAGGGA AGGCCTCCTA ACATCTCTCC AGAATCAAGT TAACCAGATT 
TTAAGTAACT GGAGAGAAGA ACGATACCAA GATGACATAA AAGCGCGGCA GATGATGCAC 
GAAGCATTGC AACTCCGCCT AAATTTGGTA GGAGGAATGT TTGACACGGT GCAGAGGAGC 
ACCCAGTGGA CTACAGACTG GGCCCTGCTA CTCCTTCAGA TCATTACTTC AGGAACTGTT 
GACATGCACA CTAACAATGA ATTATTCACA ACAGTTCTTG ACATGCTGGG TGTTTTAATC 
AATGGAACGT TAGCCTCTGA CCTATCAAAT GCATCCCCTG GGGGATCTGA AGAGAACAAG 
CGTGCATACA TGAATTTAGT AAAGAAACTG AAAAAAGAGC TAGGAGACAA GCGATCAGAA 
AGTATTGACA AAGTTCGACA GTTACTACCT TTGCCGAAAC AGACATGTGA TGTCATCACT 
TGTGAACCTA TGGGTTCCTT GATTGACACA AAAGGAAACA AAATTGCTGG ATTTGACTCT 
ATAGATAAAA AACAGGGTCT CCAGGTCTCT ACGAAGCAGA AGGTGTCCCC GTGGGACTTG 
TTTGAGGGTC AGAAGAACCC AGCTCCTTTG TCCTGGGCCT GGTTTGGGAC AGTCCGAGTG 
GACCGAAGAG TGATCAAGTA CGAGGAGCAG CATCACCTCC TGCTGTATCA CACACACCCC 
ATGCCCAAGC CCCGCAGTTA CTACCTCCAG CCACTGCCCC TGCCTCCTGA GGAGGAAGAG 
GAAGAGCCCA CATCTCCAGT TTCTCAGGAA CCAGAAAGGA AGTCCGCTGA GCTGTCAGAT 
CAGGGAAAAA CCACAACAGA TGAAGAAAAG AAAACAAAAG GAAGGAAGCG CAAGACGAAA 
TCTAGCTCAA GAGTTGATGA ATATCCACAG AGCAACATAT ACCGAGTGCC TCCTAATTAC 
TCGCCTATCT CCTCCCAAAT GATGCACCAT CCACAGTCCA CCTTGTGGGG TTACAACCTC 
GTGGGCCAGC CCCAGCAGCC CGGCTTTTTC CTTCAGAACC AATCTCTTAC TCCAGGTGGC 
TCCAGATTGG ACCCTGCAGG CTCCTTTGTC CCAACCAACA CCAAACAAGC TCTGTCAAAC 
ATGCTACAGC GGCGCTCAGG CGCCATGATG CAGCCGCCTT CTCTTCATGC AATCACATCG 
CAGCAGCAGT TGATACAGAT GAAGCTTCTG CAGCAGCAGC AGCAACAGCG ACTTCTCAGG 
CAAGCCCAGA CTCGGCCTTT CCAACAGGGC CAGCCGGGGG ACCAGGCTGC TCTCTTTGCT 
GCGCAAGCAC GGCCCTCCCC TCAGCTCCCT CAGTATCCAG GGCTGCAGCA AGCACAGACC 
ATGCCACAGG GCTATACAAT GTATGGGACA CAGATGCCTT TGCAGCAGAC ATCGCAGCAG 
CAGGCTGGCA GTGTGGTCCT GTCTCCCAGC TATAACTCCA GAGCCTATCC GGCCGCACAT 
TCCAACCCCG TGCTAATGGA AAGACTCAGA CAGATTCAGC AGCAGCCGAG TGGCTATGTT 
CAGCAGCAGG CCTCGCCGTA CCTGCAGCCC CTGACTGGCT CTCAGAGACT GAACCATCAG 
GCTCTACAGC AGAGCCCTCT GGTGGGCGGG GGAATTGATG CTGTGCTGAC TTCTGCACAT 
CCAAACCTTC CCTCCGTGCC CCTGCCTCAG GATCCCATGA GACCCAGACA GCCGCAAGTT 
CGACAGCAGC AGAGACTCCT CCAGATGCAG CAGCCCCAGC AGCCCCAGCC CCAGCAGCCT 
CCCCAGCCCC AGCAGTCCTC GCAGTCCCAG AGTCAGACCC TTGGTCTCCA AGCAATGCAG 
CCCCAGCAGC CCTTGTTTCC CAGGCAAGGC TTGCAGCAGA CCCAGCAGCA GCAGCAGACG 
GCCGCCTTGG TGCGGCAGCT CCAGAAGCAG CTTTCCAGCA ACCAGCCACA GCAAGGAGTG 
ACTCCGTATG GGCATCCTTC ACACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861170.1
CDS	401..4330
Translation	MEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSLFNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPTSPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLPSVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_017005681.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 12L (MED12L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005681.1

ORF Insert Sequence:

ATGGAGCCAG CACTGAACAT CAACGGACTA ATTGACTTCG CAATACAGTT ACTAAATGAA 
CTGAGTGTTG TGGAAGCTGA ACTGCTCCTA AAATCCTCCA GCCTGGCAGG AAGTTATACA 
ACAGGACTGT GTGTCTGCAT CGTGGCTGTT CTCAGGCGCT ATCACAGTTG TCTAATCTTG 
AATCCTGATC AGACAGCCCA GGTGTTTGAA GGGTTGTGTG GTGTGGTCAA GCATGTCGTA 
AACCCCTCAG AATGTTCTTC CCCTGAAAGA TGCATTTTAG CCTACCTCTA TGATCTCTAT 
GTGTCATGTA GCCACCTCAG AAGTAAATTT GGAGACCTCT TCAGTAGTGC CTGTTCAAAA 
GTAAAGCAAA CCATATATAA TAACGTGATG CCTGCAAATT CGAACTTGCG ATGGGATCCA 
GACTTCATGA TGGATTTTAT TGAGAATCCC TCAGCCCGCA GCATCAACTA CTCAATGCTG 
GGCAAGATCC TCAGTGACAA TGCGGCCAAT CGCTACAGCT TTGTCTGCAA TACACTCATG 
AATGTATGTA TGGGCCATCA GGATGCTGGC AGGATTAACG ACATAGCCAA TTTCTCCTCT 
GAGCTTACGG CTTGCTGCAC TGTTCTTAGT TCAGAATGGC TGGGGGTTCT GAAGGCTCTT 
TGTTGTTCTT CAAATCACGT GTGGGGGTTT AATGATGTAC TTTGCACTGT AGATGTGAGT 
GACCTTTCAT TCCATGATTC ATTAGCTACT TTCATTGCTA TTCTGATAGC ACGACAGTGT 
TTTTCCCTGG AGGACGTCGT GCAGCATGTC GCACTTCCCT CTCTTCTAGC AGCAGCTTGT 
GGGGATGCGG ACGCCGAGCC TGGGGCGAGA ATGACATGCC GACTCTTGCT TCATCTCTTC 
CGAGCTCCCC AGGCCTGCTT CTTACCTCAA GCAACGGGCA AACCTTTCCC TGGAATAAGA 
TCATCTTGTG ATAGACACCT CTTAGCCGCT GCTCACAACA GCATTGAAGT GGGAGCCGTG 
TTTGCTGTCT TAAAAGCAAT TATGATGCTT GGAGATGCCA AAATTGGCAA TAACAGTGTC 
AGCTCTTTAA AGAATGATGA CTTCACCATG AGAGGTTTGC GATGTGATGG GAATGCTGAT 
GATATCTGGA CTGCCTCACA AAATCCAAAA TCCTGTGGGA AAAGCATTTC CATAGAAACT 
GCCAATTTAA GAGAATACGC TAGATATGTA CTGAGGACTA TCTGTCAACA GGAATGGGTA 
GGAGAGCATT GCTTAAAAGA ACCTGAAAGA TTATGTACAG ACAAAGAACT TATATTGGAC 
CCTGTGCTTT CAAATATGCA AGCACAGAAA TTACTGCAGC TTATCTGTTA TCCTCATGGC 
ATTAAAGAAT GTACCGAGGG GGACAATCTG CAAAGACAGC ACATTAAGCG TATTCTTCAG 
AATCTTGAGC AGTGGACACT GAGGCAATCC TGGTTAGAAC TCCAGCTAAT GATCAAACAG 
TGCTTGAAGG ACCCTGGCTC TGGTTCTGTG GCCGAAATGA ACAACTTACT GGACAATATT 
GCAAAGGCAA CAATAGAGGT ATTCCAGCAG TCTGCAGACC TAAATAATTC TTCTAATTCT 
GGCATGAGCC TCTTCAACCC AAACAGTATT GGAAGTGCTG ATACAAGTAG CACGAGACAG 
AATGGAATAA AGACATTCCT AAGTTCCTCC GAACGCAGGG GTGTATGGTT GGTGGCCCCC 
CTCATCGCCA GGTTGCCAAC TTCTGTGCAA GGAAGAGTGC TGAAAGCCGC TGGGGAAGAG 
CTGGAGAAGG GACAGCACTT GGGTTCTTCT TCCAAAAAGG AAAGGGACAG ACAGAAACAG 
AAAAGTATGT CTCTTTTGAG TCAACAACCC TTCCTCTCAC TGGTACTTAC CTGCCTTAAG 
GGACAAGATG AACAAAGGGA AGGCCTCCTA ACATCTCTCC AGAATCAAGT TAACCAGATT 
TTAAGTAACT GGAGAGAAGA ACGATACCAA GATGACATAA AAGCGCGGCA GATGATGCAC 
GAAGCATTGC AACTCCGCCT AAATTTGGTA GGAGGAATGT TTGACACGGT GCAGAGGAGC 
ACCCAGTGGA CTACAGACTG GGCCCTGCTA CTCCTTCAGA TCATTACTTC AGGAACTGTT 
GACATGCACA CTAACAATGA ATTATTCACA ACAGTTCTTG ACATGCTGGG TGTTTTAATC 
AATGGAACGT TAGCCTCTGA CCTATCAAAT GCATCCCCTG GGGGATCTGA AGAGAACAAG 
CGTGCATACA TGAATTTAGT AAAGAAACTG AAAAAAGAGC TAGGAGACAA GCGATCAGAA 
AGTATTGACA AAGTTCGACA GTTACTACCT TTGCCGAAAC AGACATGTGA TGTCATCACT 
TGTGAACCTA TGGGTTCCTT GATTGACACA AAAGGAAACA AAATTGCTGG ATTTGACTCT 
ATAGATAAAA AACAGGGTCT CCAGGTCTCT ACGAAGCAGA AGGTGTCCCC GTGGGACTTG 
TTTGAGGGTC AGAAGAACCC AGCTCCTTTG TCCTGGGCCT GGTTTGGGAC AGTCCGAGTG 
GACCGAAGAG TGATCAAGTA CGAGGAGCAG CATCACCTCC TGCTGTATCA CACACACCCC 
ATGCCCAAGC CCCGCAGTTA CTACCTCCAG CCACTGCCCC TGCCTCCTGA GGAGGAAGAG 
GAAGAGCCCA CATCTCCAGT TTCTCAGGAA CCAGAAAGGA AGTCCGCTGA GCTGTCAGAT 
CAGGGAAAAA CCACAACAGA TGAAGAAAAG AAAACAAAAG GAAGGAAGCG CAAGACGAAA 
TCTAGCTCAA GAGTTGATGA ATATCCACAG AGCAACATAT ACCGAGTGCC TCCTAATTAC 
TCGCCTATCT CCTCCCAAAT GATGCACCAT CCACAGTCCA CCTTGTGGGG TTACAACCTC 
GTGGGCCAGC CCCAGCAGCC CGGCTTTTTC CTTCAGAACC AATCTCTTAC TCCAGGTGGC 
TCCAGATTGG ACCCTGCAGG CTCCTTTGTC CCAACCAACA CCAAACAAGC TCTGTCAAAC 
ATGCTACAGC GGCGCTCAGG CGCCATGATG CAGCCGCCTT CTCTTCATGC AATCACATCG 
CAGCAGCAGT TGATACAGAT GAAGCTTCTG CAGCAGCAGC AGCAACAGCG ACTTCTCAGG 
CAAGCCCAGA CTCGGCCTTT CCAACAGGGC CAGCCGGGGG ACCAGGCTGC TCTCTTTGCT 
GCGCAAGCAC GGCCCTCCCC TCAGCTCCCT CAGTATCCAG GGCTGCAGCA AGCACAGACC 
ATGCCACAGG GCTATACAAT GTATGGGACA CAGATGCCTT TGCAGCAGAC ATCGCAGCAG 
CAGGCTGGCA GTGTGGTCCT GTCTCCCAGC TATAACTCCA GAGCCTATCC GGCCGCACAT 
TCCAACCCCG TGCTAATGGA AAGACTCAGA CAGATTCAGC AGCAGCCGAG TGGCTATGTT 
CAGCAGCAGG CCTCGCCGTA CCTGCAGCCC CTGACTGGCT CTCAGAGACT GAACCATCAG 
GCTCTACAGC AGAGCCCTCT GGTGGGCGGG GGAATTGATG CTGTGCTGAC TTCTGCACAT 
CCAAACCTTC CCTCCGTGCC CCTGCCTCAG GATCCCATGA GACCCAGACA GCCGCAAGTT 
CGACAGCAGC AGAGACTCCT CCAGATGCAG CAGCCCCAGC AGCCCCAGCC CCAGCAGCCT 
CCCCAGCCCC AGCAGTCCTC GCAGTCCCAG AGTCAGACCC TTGGTCTCCA AGCAATGCAG 
CCCCAGCAGC CCTTGTTTCC CAGGCAAGGC TTGCAGCAGA CCCAGCAGCA GCAGCAGACG 
GCCGCCTTGG TGCGGCAGCT CCAGAAGCAG CTTTCCAGCA ACCAGCCACA GCAAGGAGTG 
ACTCCGTATG GGCATCCTTC ACACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MED12L
Entrez Gene ID	116931
Full Name	mediator complex subunit 12 like
Synonyms	NOPAR,TNRC11L,TRALP,TRALPUSH
General protein information	Preferred Names mediator complex subunit 12 like Names mediator of RNA polymerase II transcription subunit 12-like protein mediator of RNA polymerase II transcription, subunit 12 homolog-like no opposite paired repeat protein thyroid hormone receptor-associated-like protein trinucleotide repeat-containing gene 11 protein-like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q25.1
Summary	The protein encoded by this gene is part of the Mediator complex, which is involved in transcriptional coactivation of nearly all RNA polymerase II-dependent genes. The Mediator complex links gene-specific transcriptional activators with the basal transcription machinery. [provided by RefSeq, May 2010].
Disorder MIM:	611318

Homo sapiens (human)	MED12L	NP_443728.3
Pan troglodytes (chimpanzee)	MED12L	XP_516821.2
Mus musculus (house mouse)	Med12l	NP_808523.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002523	XP_312414.5
Drosophila melanogaster (fruit fly)	kto	NP_524786.1
	MED12L	XP_001107022.2
Gallus gallus (chicken)	MED12L	XP_422838.4
Canis lupus familiaris (dog)	MED12L	XP_005634712.1
Bos taurus (cattle)	MED12L	NP_001193434.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MED12L cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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