NKX2-6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NKX2-6
Entrez Gene ID	137814
Full Name	NK2 homeobox 6
Synonyms	CSX2,CTHM,NKX2F,NKX4-2
General protein information	Preferred Names NK2 homeobox 6 Names homeobox protein Nkx-2.6 NK2 transcription factor related, locus 6 homeobox protein NK2 homolog F homeobox protein NKX2.6 tinman paralog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p21.2
Summary	This gene encodes a homeobox-containing protein that belongs to the NK-2 homeobox family. This protein is a vertebrate homolog of Drosophila homeobox-containing protein called 'tinman', which has been shown to be essential for development of the heart-like dorsal vessel. In conjunction with related gene, NKX2-5, this gene may play a role in both pharyngeal and cardiac embryonic development. Mutations in this gene are associated with persistent truncus arteriosus.[provided by RefSeq, Aug 2011].
Disorder MIM:	611770
Disorder Html:	Persistent truncus arteriosus, 217095 (3); Conotruncal heart malformations, 217095 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001136271.2	NP_001129743.2	homeobox protein Nkx-2.6

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nkx2-6	NP_035050.2
Gallus gallus (chicken)	NKX2-6	NP_990468.1
Homo sapiens (human)	NKX2-6	NP_001129743.2
Pan troglodytes (chimpanzee)	NKX2-6	XP_519662.2
Canis lupus familiaris (dog)	NKX2-6	XP_543239.3
Rattus norvegicus (Norway rat)	Nkx2-6	NP_001121125.1
Xenopus tropicalis (tropical clawed frog)	nkx2-6	XP_002932599.1
	LOC717445	XP_001108914.2
Bos taurus (cattle)	NKX2-6	XP_003586459.1
Danio rerio (zebrafish)	nkx2.7	NP_571494.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel NKX2.6 mutation associated with congenital ventricular septal defect.
Wang J, Mao JH, Ding KK, Xu WJ, Liu XY, Qiu XB, Li RG, Qu XK, Xu YJ, Huang RT, Xue S, Yang YQ
Pediatric cardiology36(3)646-56(2015 Mar)

NKX2-6 mutation predisposes to familial atrial fibrillation.
Wang J, Zhang DF, Sun YM, Li RG, Qiu XB, Qu XK, Liu X, Fang WY, Yang YQ
International journal of molecular medicine34(6)1581-90(2014 Dec)

Prevalence and spectrum of Nkx2.6 mutations in patients with congenital heart disease.
Zhao L, Ni SH, Liu XY, Wei D, Yuan F, Xu L, Xin-Li, Li RG, Qu XK, Xu YJ, Fang WY, Yang YQ, Qiu XB
European journal of medical genetics57(10)579-86(2014 Oct)

Conotruncal malformations and absent thymus due to a deleterious NKX2-6 mutation.
Ta-Shma A, El-lahham N, Edvardson S, Stepensky P, Nir A, Perles Z, Gavri S, Golender J, Yaakobi-Simhayoff N, Shaag A, Rein AJ, Elpeleg O
Journal of medical genetics51(4)268-70(2014 Apr)

Beyond single-marker analyses: mining whole genome scans for insights into treatment responses in severe sepsis.
Man M, Close SL, Shaw AD, Bernard GR, Douglas IS, Kaner RJ, Payen D, Vincent JL, Fossceco S, Janes JM, Leishman AG, O'Brien L, Williams MD, Garcia JG
The pharmacogenomics journal13(3)218-26(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study firstly links NKX2.6 loss-of-function mutation with increased susceptibility to isolated VSD.
Title: A novel NKX2.6 mutation associated with congenital ventricular septal defect.

Data associated genetically defective NK2 homeobox 6 (NKX2-6) with enhanced susceptibility to atrial fibrillation (AF).
Title: NKX2-6 mutation predisposes to familial atrial fibrillation.

Heterozygous mutations in Nkx2.6, p.V176M and p.K177X, were identified in 2 unrelated patients, one with tetralogy of Fallot and one with double outlet of right ventricle and ventricular septal defect. Increased vulnerability to TOF or DORV is discussed.
Title: Prevalence and spectrum of Nkx2.6 mutations in patients with congenital heart disease.

NKX2-6 plays a role in human cardiogenesis.
Title: Conotruncal malformations and absent thymus due to a deleterious NKX2-6 mutation.

NKX2.5/NKX2.6 mutations are not a common cause of isolated type 1 truncus arteriosus in a small cohort of multiethnic cases.
Title: NKX2.5/NKX2.6 mutations are not a common cause of isolated type 1 truncus arteriosus in a small cohort of multiethnic cases.

The following NKX2-6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NKX2-6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu21269	NM_001136271.2 Latest version!	Homo sapiens NK2 homeobox 6 (NKX2-6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NKX2-6 ( NM_001136271.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129743.2 Homo sapiens NK2 homeobox 6 (NKX2-6), mRNA.

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CloneID	OHu21269
Clone ID Related Accession (Same CDS sequence)	NM_001136271.2
Accession Version	NM_001136271.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	homeobox protein Nkx-2.6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012574.7 and CN272646.1. This sequence is a reference standard in the RefSeqGene project. On Aug 10, 2011 this sequence version replaced NM_001136271.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments, and homology data from other species. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN272646.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGCTGCTGA GCCCCGTCAC CTCCACCCCC TTCTCGGTCA AGGACATCCT GCGACTGGAG 
CGCGAGCGGA GCTGCCCCGC GGCTTCGCCA CATCCGCGGG TGCGGAAGAG CCCGGAAAAC 
TTTCAGTACC TGAGAATGGA CGCAGAGCCG CGAGGGTCAG AGGTTCACAA CGCTGGTGGC 
GGCGGCGGTG ACAGAAAGCT GGATGGTTCG GAGCCTCCTG GGGGTCCCTG TGAGGCAGTC 
TTGGAGATGG ACGCGGAACG GATGGGGGAG CCACAGCCCG GCCTGAACGC GGCCTCGCCC 
CTCGGCGGCG GGACCAGGGT GCCAGAGCGC GGCGTTGGCA ACAGCGGCGA CAGCGTGCGG 
GGTGGCCGCT CGGAGCAGCC CAAGGCGCGG CAACGACGGA AGCCGCGCGT GCTCTTTTCG 
CAGGCGCAGG TGCTGGCCCT GGAGCGGCGC TTCAAGCAGC AGCGGTACCT GTCAGCGCCC 
GAGCGCGAGC ACCTGGCCAG CGCGCTGCAG CTCACGTCCA CGCAGGTCAA GATCTGGTTC 
CAGAACCGAC GCTACAAATG CAAGAGACAG CGCCAGGACA AGTCGCTGGA ACTGGCTGGC 
CACCCTCTAA CGCCGCGCCG AGTAGCTGTG CCCGTCCTGG TGCGCGATGG CAAGCCCTGC 
CTGGGCCCCG GGCCCGGCGC ACCTGCCTTC CCCAGCCCCT ACAGTGCAGC AGTGTCGCCC 
TACTCTTGCT ACGGAGGCTA CAGCGGAGCA CCCTACGGCG CAGGCTACGG CACCTGCTAC 
GCGGGCGCGC CCTCGGGTCC TGCGCCACAC ACACCACTGG CCAGCGCGGG CTTCGGACAC 
GGTGGCCAGA ATGCCACCCC GCAGGGCCAT CTGGCAGCCA CGCTGCAGGG TGTCAGGGCC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129743.2
CDS	1..906
Translation	MLLSPVTSTPFSVKDILRLERERSCPAASPHPRVRKSPENFQYLRMDAEPRGSEVHNAGGGGGDRKLDGSEPPGGPCEAVLEMDAERMGEPQPGLNAASPLGGGTRVPERGVGNSGDSVRGGRSEQPKARQRRKPRVLFSQAQVLALERRFKQQRYLSAPEREHLASALQLTSTQVKIWFQNRRYKCKRQRQDKSLELAGHPLTPRRVAVPVLVRDGKPCLGPGPGAPAFPSPYSAAVSPYSCYGGYSGAPYGAGYGTCYAGAPSGPAPHTPLASAGFGHGGQNATPQGHLAATLQGVRAW

Target ORF information:

RefSeq Version	NM_001136271.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NK2 homeobox 6 (NKX2-6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136271.2

ORF Insert Sequence:

ATGCTGCTGA GCCCCGTCAC CTCCACCCCC TTCTCGGTCA AGGACATCCT GCGACTGGAG 
CGCGAGCGGA GCTGCCCCGC GGCTTCGCCA CATCCGCGGG TGCGGAAGAG CCCGGAAAAC 
TTTCAGTACC TGAGAATGGA CGCAGAGCCG CGAGGGTCAG AGGTTCACAA CGCTGGTGGC 
GGCGGCGGTG ACAGAAAGCT GGATGGTTCG GAGCCTCCTG GGGGTCCCTG TGAGGCAGTC 
TTGGAGATGG ACGCGGAACG GATGGGGGAG CCACAGCCCG GCCTGAACGC GGCCTCGCCC 
CTCGGCGGCG GGACCAGGGT GCCAGAGCGC GGCGTTGGCA ACAGCGGCGA CAGCGTGCGG 
GGTGGCCGCT CGGAGCAGCC CAAGGCGCGG CAACGACGGA AGCCGCGCGT GCTCTTTTCG 
CAGGCGCAGG TGCTGGCCCT GGAGCGGCGC TTCAAGCAGC AGCGGTACCT GTCAGCGCCC 
GAGCGCGAGC ACCTGGCCAG CGCGCTGCAG CTCACGTCCA CGCAGGTCAA GATCTGGTTC 
CAGAACCGAC GCTACAAATG CAAGAGACAG CGCCAGGACA AGTCGCTGGA ACTGGCTGGC 
CACCCTCTAA CGCCGCGCCG AGTAGCTGTG CCCGTCCTGG TGCGCGATGG CAAGCCCTGC 
CTGGGCCCCG GGCCCGGCGC ACCTGCCTTC CCCAGCCCCT ACAGTGCAGC AGTGTCGCCC 
TACTCTTGCT ACGGAGGCTA CAGCGGAGCA CCCTACGGCG CAGGCTACGG CACCTGCTAC 
GCGGGCGCGC CCTCGGGTCC TGCGCCACAC ACACCACTGG CCAGCGCGGG CTTCGGACAC 
GGTGGCCAGA ATGCCACCCC GCAGGGCCAT CTGGCAGCCA CGCTGCAGGG TGTCAGGGCC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel NKX2.6 mutation associated with congenital ventricular septal defect.
Pediatric cardiology36(3)646-56(2015 Mar)
Wang J,Mao JH,Ding KK,Xu WJ,Liu XY,Qiu XB,Li RG,Qu XK,Xu YJ,Huang RT,Xue S,Yang YQ

NKX2-6 mutation predisposes to familial atrial fibrillation.
International journal of molecular medicine34(6)1581-90(2014 Dec)
Wang J,Zhang DF,Sun YM,Li RG,Qiu XB,Qu XK,Liu X,Fang WY,Yang YQ

Prevalence and spectrum of Nkx2.6 mutations in patients with congenital heart disease.
European journal of medical genetics57(10)579-86(2014 Oct)
Zhao L,Ni SH,Liu XY,Wei D,Yuan F,Xu L,Xin-Li,Li RG,Qu XK,Xu YJ,Fang WY,Yang YQ,Qiu XB

Conotruncal malformations and absent thymus due to a deleterious NKX2-6 mutation.
Journal of medical genetics51(4)268-70(2014 Apr)
Ta-Shma A,El-lahham N,Edvardson S,Stepensky P,Nir A,Perles Z,Gavri S,Golender J,Yaakobi-Simhayoff N,Shaag A,Rein AJ,Elpeleg O

Beyond single-marker analyses: mining whole genome scans for insights into treatment responses in severe sepsis.
The pharmacogenomics journal13(3)218-26(2013 Jun)
Man M,Close SL,Shaw AD,Bernard GR,Douglas IS,Kaner RJ,Payen D,Vincent JL,Fossceco S,Janes JM,Leishman AG,O'Brien L,Williams MD,Garcia JG

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NKX2-6 cDNA ORF clone, Homo sapiens(human)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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