×

DCTN1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol DCTN1
Entrez Gene ID 1639
Full Name dynactin 1
Synonyms DAP-150, DP-150, P135
General protein information
Preferred Names
dynactin subunit 1
Names
dynactin subunit 1
150 kDa dynein-associated polypeptide
dynactin 1 (p150, glued homolog, Drosophila)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p13

Summary This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]. lac of sum
Disorder MIM:

601143

Disorder Html: Neuropathy, distal hereditary motor, type VIIB, 607641 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001190836 NP_001177765 dynactin subunit 1 isoform 5
NM_001190837 NP_001177766 dynactin subunit 1 isoform 6
NM_001135040 NP_001128512 dynactin subunit 1 isoform 3
NM_001135041 NP_001128513 dynactin subunit 1 isoform 4
NM_004082 NP_004073 dynactin subunit 1 isoform 1
NM_023019 NP_075408 dynactin subunit 1 isoform 2



Homo sapiens (human) DCTN1 NP_004073.2
Pan troglodytes (chimpanzee) DCTN1 XP_001156535.1
Macaca mulatta (Rhesus monkey) DCTN1 XP_001108486.2
Canis lupus familiaris (dog) DCTN1 XP_005630593.1
Bos taurus (cattle) DCTN1 NP_001092404.1
Mus musculus (house mouse) Dctn1 NP_031861.2
Rattus norvegicus (Norway rat) Dctn1 NP_077044.1
Gallus gallus (chicken) DCTN1 NP_001026538.1
Danio rerio (zebrafish) dctn1b XP_001342673.3
Drosophila melanogaster (fruit fly) Gl NP_524061.1
Xenopus (Silurana) tropicalis (western clawed frog) dctn1 XP_002936330.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DCTN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCTN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Free ORF Clone
for Every Scientist
More Detail >>

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu16693 NM_001190836 Homo sapiens dynactin 1 (DCTN1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu16952 NM_001190837 Homo sapiens dynactin 1 (DCTN1), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu16796 NM_001135040 Homo sapiens dynactin 1 (DCTN1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu16697 NM_001135041 Homo sapiens dynactin 1 (DCTN1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu18647
NM_004082 Homo sapiens dynactin 1 (DCTN1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$459.50-$643.30
$919.00
OHu16728 NM_023019 Homo sapiens dynactin 1 (DCTN1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16693
Clone ID Related Accession (Same CDS sequence) NM_001190836
Accession Version NM_001190836.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dynactin subunit 1 isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC309469.1, BC013978.2, AK297286.1 and CA448857.1. Transcript Variant: This variant (5) has multiple differences in the 5' end, compared to variant 4. These differences cause translation initiation from an upstream AUG and a protein (isoform 5) with a longer N-terminus, compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013978.2, AK297286.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGAGTGCGG AGGCAAGCGC CCGGCCTCTG CGGGTGGGCT CCCGTGTAGA GGTGATTGGA 
AAAGGCCACC GAGGCACTGT GGCCTATGTT GGAGCCACAC TGTTTGCCAC TGGCAAATGG
GTAGGCGTGA TTCTGGATGA AGCAAAGGGC AAAAATGATG GAACTGTTCA AGGCAGGAAG
TACTTCACTT GTGATGAAGG GCATGGCATC TTTGTGCGCC AGTCCCAGAT CCAGGTATTT
GAAGATGGAG CAGATACTAC TTCCCCAGAG ACACCTGATT CTTCTGCTTC AAAAGTCCTC
AAAAGAGAGG GAACTGATAC AACTGCAAAG ACTAGCAAAC TGCCCACGCG CCCAGCCAGT
ACTGGGGTGG CTGGGGCCAG TAGCTCCCTG GGCCCCTCTG GCTCAGCGTC AGCAGGTGAG
CTGAGCAGCA GTGAGCCCAG CACCCCGGCT CAGACTCCGC TGGCAGCACC CATCATCCCC
ACGCCGGTCC TCACCTCTCC TGGAGCAGTC CCCCCGCTTC CTTCCCCATC CAAGGAGGAG
GAGGGACTAA GGGCTCAGGT GCGGGACCTG GAGGAGAAAC TAGAGACCCT GAGACTGAAA
CGGGCAGAAG ACAAAGCAAA GCTAAAAGAG CTGGAGAAAC ACAAAATCCA GCTGGAGCAG
GTGCAGGAAT GGAAGAGCAA AATGCAGGAG CAGCAGGCCG ACCTGCAGCG GCGCCTCAAG
GAGGCGAGAA AGGAAGCCAA GGAGGCGCTG GAGGCAAAGG AACGCTATAT GGAGGAGATG
GCTGATACTG CTGATGCCAT TGAGATGGCC ACTTTGGACA AGGAGATGGC TGAAGAGCGG
GCTGAGTCCC TGCAGCAGGA GGTGGAGGCA CTGAAGGAGC GGGTGGACGA GCTCACTACT
GACTTAGAGA TCCTCAAGGC TGAGATTGAA GAGAAGGGCT CAGATGGCGC TGCATCCAGT
TATCAGCTCA AGCAGCTTGA GGAGCAGAAT GCCCGCCTGA AGGATGCCCT GGTGAGGATG
CGGGATCTTT CTTCCTCAGA GAAGCAGGAG CATGTGAAGC TCCAGAAGCT CATGGAAAAG
AAGAACCAAG AGCTGGAAGT TGTGAGGCAA CAGCGGGAGC GTCTGCAGGA GGAGCTAAGC
CAGGCAGAGA GCACCATTGA TGAGCTCAAG GAGCAGGTGG ATGCTGCTCT GGGTGCTGAG
GAGATGGTGG AGATGCTGAC AGATCGGAAC CTGAATCTGG AAGAGAAAGT GCGCGAGTTG
AGGGAGACTG TGGGAGACTT GGAAGCGATG AATGAGATGA ACGATGAGCT GCAGGAGAAT
GCACGTGAGA CAGAACTGGA GCTGCGGGAG CAGCTGGACA TGGCAGGCGC GCGGGTTCGT
GAGGCCCAGA AGCGTGTGGA GGCAGCCCAG GAGACGGTTG CAGACTACCA GCAGACCATC
AAGAAGTACC GCCAGCTGAC CGCCCATCTA CAGGATGTGA ATCGGGAACT GACAAACCAG
CAGGAAGCAT CTGTGGAGAG GCAACAGCAG CCACCTCCAG AGACCTTTGA CTTCAAAATC
AAGTTTGCTG AGACTAAGGC CCATGCCAAG GCAATTGAGA TGGAATTGAG GCAGATGGAG
GTGGCCCAGG CCAATCGACA CATGTCCCTG CTGACAGCCT TCATGCCTGA CAGCTTCCTT
CGGCCAGGTG GGGACCATGA CTGCGTTCTG GTGCTGTTGC TCATGCCTCG TCTCATTTGC
AAGGCAGAGC TGATCCGGAA GCAGGCCCAG GAGAAGTTTG AACTAAGTGA GAACTGTTCA
GAGCGGCCTG GGCTGCGAGG AGCTGCTGGG GAGCAACTCA GCTTTGCTGC TGGACTGGTG
TACTCGCTGA GCCTGCTGCA GGCCACGCTA CACCGCTATG AGCATGCCCT CTCTCAGTGC
AGTGTGGATG TGTATAAGAA AGTGGGCAGC CTGTACCCTG AGATGAGTGC CCATGAGCGC
TCCTTGGATT TCCTCATTGA ACTGCTGCAC AAGGATCAGC TGGATGAGAC TGTCAATGTG
GAGCCTCTCA CCAAGGCCAT CAAGTACTAT CAGCATCTGT ACAGCATCCA CCTTGCCGAA
CAGCCTGAGG ACTGTACTAT GCAGCTGGCT GACCACATTA AGTTCACGCA GAGTGCTCTG
GACTGCATGA GTGTGGAGGT AGGACGGCTG CGTGCCTTCT TGCAGGGTGG GCAGGAGGCT
ACAGATATTG CCCTCCTGCT CCGGGATCTG GAAACTTCAT GCAGTGACAT CCGCCAGTTC
TGCAAGAAGA TCCGAAGGCG AATGCCAGGG ACAGATGCTC CTGGGATCCC AGCTGCACTG
GCCTTTGGAC CACAGGTATC TGACACGCTC CTAGACTGCA GGAAACACTT GACGTGGGTC
GTGGCTGTGC TGCAGGAGGT GGCAGCTGCT GCTGCCCAGC TCATTGCCCC ACTGGCAGAG
AATGAGGGGC TACTTGTGGC TGCTCTGGAG GAACTGGCTT TCAAAGCAAG CGAGCAGATC
TATGGGACCC CCTCCAGCAG CCCCTATGAG TGTCTGCGCC AGTCATGCAA CATCCTCATC
AGTACCATGA ACAAGCTGGC CACAGCCATG CAGGAGGGGG AGTATGATGC AGAGCGGCCC
CCCAGCAAGC CTCCACCGGT TGAACTGCGG GCTGCTGCCC TTCGTGCAGA GATCACAGAT
GCTGAAGGCC TGGGTTTGAA GCTCGAAGAT CGAGAGACAG TTATTAAGGA GTTGAAGAAG
TCACTCAAGA TTAAGGGAGA GGAGCTAAGT GAGGCCAATG TGCGGCTGAG CCTCCTGGAG
AAGAAGTTGG ACAGTGCTGC CAAGGATGCA GATGAGCGCA TCGAGAAAGT CCAGACTCGG
CTGGAGGAGA CCCAGGCACT GCTGCGAAAG AAGGAGAAAG AGTTTGAGGA GACAATGGAT
GCACTCCAGG CTGACATCGA CCAGCTGGAG GCAGAGAAGG CAGAACTAAA GCAGCGTCTG
AACAGCCAGT CCAAACGCAC GATTGAGGGA CTCCGGGGCC CTCCTCCTTC AGGCATTGCT
ACTCTGGTCT CTGGCATTGC TGGTGGAGCC ATCCCTGGGC AGGCTCCAGG GTCTGTGCCA
GGCCCAGGGC TGGTGAAGGA CTCACCACTG CTGCTTCAGC AGATCTCTGC CATGAGGCTG
CACATCTCCC AGCTCCAGCA TGAGAACAGC ATCCTCAAGG GAGCCCAGAT GAAGGCATCC
TTGGCATCCC TGCCCCCTCT GCATGTTGCA AAGCTATCCC ATGAGGGCCC TGGCAGTGAG
TTACCAGCTG GAGCGCTGTA TCGTAAGACC AGCCAGCTGC TGGAGACATT GAATCAATTG
AGCACACACA CGCACGTAGT AGACATCACT CGCACCAGCC CTGCTGCCAA GAGCCCGTCG
GCCCAACTTA TGGAGCAAGT GGCTCAGCTT AAGTCCCTGA GTGACACCGT CGAGAAGCTC
AAGGATGAGG TCCTCAAGGA GACAGTATCT CAGCGCCCTG GAGCCACAGT ACCCACTGAC
TTTGCCACCT TCCCTTCATC AGCCTTCCTC AGGGCCAAGG AGGAGCAGCA GGATGACACA
GTCTACATGG GCAAAGTGAC CTTCTCATGT GCGGCTGGTT TTGGACAGCG ACACCGGCTG
GTGCTGACCC AGGAGCAGCT GCACCAGCTT CACAGTCGCC TCATCTCCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001177765.1
CDS313..4023
Misc Feature(1)346..546(+)
Misc Feature(2)832..>1191(+)
Misc Feature(3)937..1323(+)
Misc Feature(4)955..1494(+)
Misc Feature(5)1141..1698(+)
Misc Feature(6)1777..2616(+)
Misc Feature(7)3118..3345(+)
Exon (1)1..294
Gene:DCTN1
Gene Synonym:
Exon (2)295..540
Gene:DCTN1
Gene Synonym:
Exon (3)541..619
Gene:DCTN1
Gene Synonym:
Exon (4)620..654
Gene:DCTN1
Gene Synonym:
Exon (5)655..846
Gene:DCTN1
Gene Synonym:
Exon (6)847..1044
Gene:DCTN1
Gene Synonym:
Exon (7)1045..1249
Gene:DCTN1
Gene Synonym:
Exon (8)1250..1328
Gene:DCTN1
Gene Synonym:
Exon (9)1329..1488
Gene:DCTN1
Gene Synonym:
Exon (10)1489..1593
Gene:DCTN1
Gene Synonym:
Exon (11)1594..1785
Gene:DCTN1
Gene Synonym:
Exon (12)1786..1902
Gene:DCTN1
Gene Synonym:
Exon (13)1903..2055
Gene:DCTN1
Gene Synonym:
Exon (14)2056..2216
Gene:DCTN1
Gene Synonym:
Exon (15)2217..2385
Gene:DCTN1
Gene Synonym:
Exon (16)2386..2454
Gene:DCTN1
Gene Synonym:
Exon (17)2455..2517
Gene:DCTN1
Gene Synonym:
Exon (18)2518..2667
Gene:DCTN1
Gene Synonym:
Exon (19)2668..2829
Gene:DCTN1
Gene Synonym:
Exon (20)2830..2961
Gene:DCTN1
Gene Synonym:
Exon (21)2962..3087
Gene:DCTN1
Gene Synonym:
Exon (22)3088..3230
Gene:DCTN1
Gene Synonym:
Exon (23)3231..3397
Gene:DCTN1
Gene Synonym:
Exon (24)3398..3531
Gene:DCTN1
Gene Synonym:
Exon (25)3532..3715
Gene:DCTN1
Gene Synonym:
Exon (26)3716..3795
Gene:DCTN1
Gene Synonym:
Exon (27)3796..3885
Gene:DCTN1
Gene Synonym:
Exon (28)3886..4368
Gene:DCTN1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001190836
Organism Homo sapiens (human)
Definition Homo sapiens dynactin 1 (DCTN1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001190836

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGAGTGCGG AGGCAAGCGC CCGGCCTCTG CGGGTGGGCT CCCGTGTAGA GGTGATTGGA 
AAAGGCCACC GAGGCACTGT GGCCTATGTT GGAGCCACAC TGTTTGCCAC TGGCAAATGG
GTAGGCGTGA TTCTGGATGA AGCAAAGGGC AAAAATGATG GAACTGTTCA AGGCAGGAAG
TACTTCACTT GTGATGAAGG GCATGGCATC TTTGTGCGCC AGTCCCAGAT CCAGGTATTT
GAAGATGGAG CAGATACTAC TTCCCCAGAG ACACCTGATT CTTCTGCTTC AAAAGTCCTC
AAAAGAGAGG GAACTGATAC AACTGCAAAG ACTAGCAAAC TGCCCACGCG CCCAGCCAGT
ACTGGGGTGG CTGGGGCCAG TAGCTCCCTG GGCCCCTCTG GCTCAGCGTC AGCAGGTGAG
CTGAGCAGCA GTGAGCCCAG CACCCCGGCT CAGACTCCGC TGGCAGCACC CATCATCCCC
ACGCCGGTCC TCACCTCTCC TGGAGCAGTC CCCCCGCTTC CTTCCCCATC CAAGGAGGAG
GAGGGACTAA GGGCTCAGGT GCGGGACCTG GAGGAGAAAC TAGAGACCCT GAGACTGAAA
CGGGCAGAAG ACAAAGCAAA GCTAAAAGAG CTGGAGAAAC ACAAAATCCA GCTGGAGCAG
GTGCAGGAAT GGAAGAGCAA AATGCAGGAG CAGCAGGCCG ACCTGCAGCG GCGCCTCAAG
GAGGCGAGAA AGGAAGCCAA GGAGGCGCTG GAGGCAAAGG AACGCTATAT GGAGGAGATG
GCTGATACTG CTGATGCCAT TGAGATGGCC ACTTTGGACA AGGAGATGGC TGAAGAGCGG
GCTGAGTCCC TGCAGCAGGA GGTGGAGGCA CTGAAGGAGC GGGTGGACGA GCTCACTACT
GACTTAGAGA TCCTCAAGGC TGAGATTGAA GAGAAGGGCT CAGATGGCGC TGCATCCAGT
TATCAGCTCA AGCAGCTTGA GGAGCAGAAT GCCCGCCTGA AGGATGCCCT GGTGAGGATG
CGGGATCTTT CTTCCTCAGA GAAGCAGGAG CATGTGAAGC TCCAGAAGCT CATGGAAAAG
AAGAACCAAG AGCTGGAAGT TGTGAGGCAA CAGCGGGAGC GTCTGCAGGA GGAGCTAAGC
CAGGCAGAGA GCACCATTGA TGAGCTCAAG GAGCAGGTGG ATGCTGCTCT GGGTGCTGAG
GAGATGGTGG AGATGCTGAC AGATCGGAAC CTGAATCTGG AAGAGAAAGT GCGCGAGTTG
AGGGAGACTG TGGGAGACTT GGAAGCGATG AATGAGATGA ACGATGAGCT GCAGGAGAAT
GCACGTGAGA CAGAACTGGA GCTGCGGGAG CAGCTGGACA TGGCAGGCGC GCGGGTTCGT
GAGGCCCAGA AGCGTGTGGA GGCAGCCCAG GAGACGGTTG CAGACTACCA GCAGACCATC
AAGAAGTACC GCCAGCTGAC CGCCCATCTA CAGGATGTGA ATCGGGAACT GACAAACCAG
CAGGAAGCAT CTGTGGAGAG GCAACAGCAG CCACCTCCAG AGACCTTTGA CTTCAAAATC
AAGTTTGCTG AGACTAAGGC CCATGCCAAG GCAATTGAGA TGGAATTGAG GCAGATGGAG
GTGGCCCAGG CCAATCGACA CATGTCCCTG CTGACAGCCT TCATGCCTGA CAGCTTCCTT
CGGCCAGGTG GGGACCATGA CTGCGTTCTG GTGCTGTTGC TCATGCCTCG TCTCATTTGC
AAGGCAGAGC TGATCCGGAA GCAGGCCCAG GAGAAGTTTG AACTAAGTGA GAACTGTTCA
GAGCGGCCTG GGCTGCGAGG AGCTGCTGGG GAGCAACTCA GCTTTGCTGC TGGACTGGTG
TACTCGCTGA GCCTGCTGCA GGCCACGCTA CACCGCTATG AGCATGCCCT CTCTCAGTGC
AGTGTGGATG TGTATAAGAA AGTGGGCAGC CTGTACCCTG AGATGAGTGC CCATGAGCGC
TCCTTGGATT TCCTCATTGA ACTGCTGCAC AAGGATCAGC TGGATGAGAC TGTCAATGTG
GAGCCTCTCA CCAAGGCCAT CAAGTACTAT CAGCATCTGT ACAGCATCCA CCTTGCCGAA
CAGCCTGAGG ACTGTACTAT GCAGCTGGCT GACCACATTA AGTTCACGCA GAGTGCTCTG
GACTGCATGA GTGTGGAGGT AGGACGGCTG CGTGCCTTCT TGCAGGGTGG GCAGGAGGCT
ACAGATATTG CCCTCCTGCT CCGGGATCTG GAAACTTCAT GCAGTGACAT CCGCCAGTTC
TGCAAGAAGA TCCGAAGGCG AATGCCAGGG ACAGATGCTC CTGGGATCCC AGCTGCACTG
GCCTTTGGAC CACAGGTATC TGACACGCTC CTAGACTGCA GGAAACACTT GACGTGGGTC
GTGGCTGTGC TGCAGGAGGT GGCAGCTGCT GCTGCCCAGC TCATTGCCCC ACTGGCAGAG
AATGAGGGGC TACTTGTGGC TGCTCTGGAG GAACTGGCTT TCAAAGCAAG CGAGCAGATC
TATGGGACCC CCTCCAGCAG CCCCTATGAG TGTCTGCGCC AGTCATGCAA CATCCTCATC
AGTACCATGA ACAAGCTGGC CACAGCCATG CAGGAGGGGG AGTATGATGC AGAGCGGCCC
CCCAGCAAGC CTCCACCGGT TGAACTGCGG GCTGCTGCCC TTCGTGCAGA GATCACAGAT
GCTGAAGGCC TGGGTTTGAA GCTCGAAGAT CGAGAGACAG TTATTAAGGA GTTGAAGAAG
TCACTCAAGA TTAAGGGAGA GGAGCTAAGT GAGGCCAATG TGCGGCTGAG CCTCCTGGAG
AAGAAGTTGG ACAGTGCTGC CAAGGATGCA GATGAGCGCA TCGAGAAAGT CCAGACTCGG
CTGGAGGAGA CCCAGGCACT GCTGCGAAAG AAGGAGAAAG AGTTTGAGGA GACAATGGAT
GCACTCCAGG CTGACATCGA CCAGCTGGAG GCAGAGAAGG CAGAACTAAA GCAGCGTCTG
AACAGCCAGT CCAAACGCAC GATTGAGGGA CTCCGGGGCC CTCCTCCTTC AGGCATTGCT
ACTCTGGTCT CTGGCATTGC TGGTGGAGCC ATCCCTGGGC AGGCTCCAGG GTCTGTGCCA
GGCCCAGGGC TGGTGAAGGA CTCACCACTG CTGCTTCAGC AGATCTCTGC CATGAGGCTG
CACATCTCCC AGCTCCAGCA TGAGAACAGC ATCCTCAAGG GAGCCCAGAT GAAGGCATCC
TTGGCATCCC TGCCCCCTCT GCATGTTGCA AAGCTATCCC ATGAGGGCCC TGGCAGTGAG
TTACCAGCTG GAGCGCTGTA TCGTAAGACC AGCCAGCTGC TGGAGACATT GAATCAATTG
AGCACACACA CGCACGTAGT AGACATCACT CGCACCAGCC CTGCTGCCAA GAGCCCGTCG
GCCCAACTTA TGGAGCAAGT GGCTCAGCTT AAGTCCCTGA GTGACACCGT CGAGAAGCTC
AAGGATGAGG TCCTCAAGGA GACAGTATCT CAGCGCCCTG GAGCCACAGT ACCCACTGAC
TTTGCCACCT TCCCTTCATC AGCCTTCCTC AGGGCCAAGG AGGAGCAGCA GGATGACACA
GTCTACATGG GCAAAGTGAC CTTCTCATGT GCGGCTGGTT TTGGACAGCG ACACCGGCTG
GTGCTGACCC AGGAGCAGCT GCACCAGCTT CACAGTCGCC TCATCTCCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16952
Clone ID Related Accession (Same CDS sequence) NM_001190837
Accession Version NM_001190837.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dynactin subunit 1 isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA315227.1, AK314352.1, X98801.1, BX640799.1 and CA448857.1. Transcript Variant: This variant (6) has multiple differences in the 5' end and in the 3' coding region, compared to variant 4. These differences cause translation initiation from an upstream AUG and a protein (isoform 6) with longer N- and C-termini, compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGGCACCGA CAGCCCGAAA GACCACAACT
CGGCGACCCA AGCCCACGCG CCCAGCCAGT ACTGGGGTGG CTGGGGCCAG TAGCTCCCTG
GGCCCCTCTG GCTCAGCGTC AGCAGGTGAG CTGAGCAGCA GTGAGCCCAG CACCCCGGCT
CAGACTCCGC TGGCAGCACC CATCATCCCC ACGCCGGTCC TCACCTCTCC TGGAGCAGTC
CCCCCGCTTC CTTCCCCATC CAAGGAGGAG GAGGGACTAA GGGCTCAGGT GCGGGACCTG
GAGGAGAAAC TAGAGACCCT GAGACTGAAA CGGGCAGAAG ACAAAGCAAA GCTAAAAGAG
CTGGAGAAAC ACAAAATCCA GCTGGAGCAG GTGCAGGAAT GGAAGAGCAA AATGCAGGAG
CAGCAGGCCG ACCTGCAGCG GCGCCTCAAG GAGGCGAGAA AGGAAGCCAA GGAGGCGCTG
GAGGCAAAGG AACGCTATAT GGAGGAGATG GCTGATACTG CTGATGCCAT TGAGATGGCC
ACTTTGGACA AGGAGATGGC TGAAGAGCGG GCTGAGTCCC TGCAGCAGGA GGTGGAGGCA
CTGAAGGAGC GGGTGGACGA GCTCACTACT GACTTAGAGA TCCTCAAGGC TGAGATTGAA
GAGAAGGGCT CAGATGGCGC TGCATCCAGT TATCAGCTCA AGCAGCTTGA GGAGCAGAAT
GCCCGCCTGA AGGATGCCCT GGTGAGGATG CGGGATCTTT CTTCCTCAGA GAAGCAGGAG
CATGTGAAGC TCCAGAAGCT CATGGAAAAG AAGAACCAAG AGCTGGAAGT TGTGAGGCAA
CAGCGGGAGC GTCTGCAGGA GGAGCTAAGC CAGGCAGAGA GCACCATTGA TGAGCTCAAG
GAGCAGGTGG ATGCTGCTCT GGGTGCTGAG GAGATGGTGG AGATGCTGAC AGATCGGAAC
CTGAATCTGG AAGAGAAAGT GCGCGAGTTG AGGGAGACTG TGGGAGACTT GGAAGCGATG
AATGAGATGA ACGATGAGCT GCAGGAGAAT GCACGTGAGA CAGAACTGGA GCTGCGGGAG
CAGCTGGACA TGGCAGGCGC GCGGGTTCGT GAGGCCCAGA AGCGTGTGGA GGCAGCCCAG
GAGACGGTTG CAGACTACCA GCAGACCATC AAGAAGTACC GCCAGCTGAC CGCCCATCTA
CAGGATGTGA ATCGGGAACT GACAAACCAG CAGGAAGCAT CTGTGGAGAG GCAACAGCAG
CCACCTCCAG AGACCTTTGA CTTCAAAATC AAGTTTGCTG AGACTAAGGC CCATGCCAAG
GCAATTGAGA TGGAATTGAG GCAGATGGAG GTGGCCCAGG CCAATCGACA CATGTCCCTG
CTGACAGCCT TCATGCCTGA CAGCTTCCTT CGGCCAGGTG GGGACCATGA CTGCGTTCTG
GTGCTGTTGC TCATGCCTCG TCTCATTTGC AAGGCAGAGC TGATCCGGAA GCAGGCCCAG
GAGAAGTTTG AACTAAGTGA GAACTGTTCA GAGCGGCCTG GGCTGCGAGG AGCTGCTGGG
GAGCAACTCA GCTTTGCTGC TGGACTGGTG TACTCGCTGA GCCTGCTGCA GGCCACGCTA
CACCGCTATG AGCATGCCCT CTCTCAGTGC AGTGTGGATG TGTATAAGAA AGTGGGCAGC
CTGTACCCTG AGATGAGTGC CCATGAGCGC TCCTTGGATT TCCTCATTGA ACTGCTGCAC
AAGGATCAGC TGGATGAGAC TGTCAATGTG GAGCCTCTCA CCAAGGCCAT CAAGTACTAT
CAGCATCTGT ACAGCATCCA CCTTGCCGAA CAGCCTGAGG ACTGTACTAT GCAGCTGGCT
GACCACATTA AGTTCACGCA GAGTGCTCTG GACTGCATGA GTGTGGAGGT AGGACGGCTG
CGTGCCTTCT TGCAGGGTGG GCAGGAGGCT ACAGATATTG CCCTCCTGCT CCGGGATCTG
GAAACTTCAT GCAGTGACAT CCGCCAGTTC TGCAAGAAGA TCCGAAGGCG AATGCCAGGG
ACAGATGCTC CTGGGATCCC AGCTGCACTG GCCTTTGGAC CACAGGTATC TGACACGCTC
CTAGACTGCA GGAAACACTT GACGTGGGTC GTGGCTGTGC TGCAGGAGGT GGCAGCTGCT
GCTGCCCAGC TCATTGCCCC ACTGGCAGAG AATGAGGGGC TACTTGTGGC TGCTCTGGAG
GAACTGGCTT TCAAAGCAAG CGAGCAGATC TATGGGACCC CCTCCAGCAG CCCCTATGAG
TGTCTGCGCC AGTCATGCAA CATCCTCATC AGTACCATGA ACAAGCTGGC CACAGCCATG
CAGGAGGGGG AGTATGATGC AGAGCGGCCC CCCAGCAAGC CTCCACCGGT TGAACTGCGG
GCTGCTGCCC TTCGTGCAGA GATCACAGAT GCTGAAGGCC TGGGTTTGAA GCTCGAAGAT
CGAGAGACAG TTATTAAGGA GTTGAAGAAG TCACTCAAGA TTAAGGGAGA GGAGCTAAGT
GAGGCCAATG TGCGGCTGAG CCTCCTGGAG AAGAAGTTGG ACAGTGCTGC CAAGGATGCA
GATGAGCGCA TCGAGAAAGT CCAGACTCGG CTGGAGGAGA CCCAGGCACT GCTGCGAAAG
AAGGAGAAAG AGTTTGAGGA GACAATGGAT GCACTCCAGG CTGACATCGA CCAGCTGGAG
GCAGAGAAGG CAGAACTAAA GCAGCGTCTG AACAGCCAGT CCAAACGCAC GATTGAGGGA
CTCCGGGGCC CTCCTCCTTC AGGCATTGCT ACTCTGGTCT CTGGCATTGC TGGTGAAGAA
CAGCAGCGAG GAGCCATCCC TGGGCAGGCT CCAGGGTCTG TGCCAGGCCC AGGGCTGGTG
AAGGACTCAC CACTGCTGCT TCAGCAGATC TCTGCCATGA GGCTGCACAT CTCCCAGCTC
CAGCATGAGA ACAGCATCCT CAAGGGAGCC CAGATGAAGG CATCCTTGGC ATCCCTGCCC
CCTCTGCATG TTGCAAAGCT ATCCCATGAG GGCCCTGGCA GTGAGTTACC AGCTGGAGCG
CTGTATCGTA AGACCAGCCA GCTGCTGGAG ACATTGAATC AATTGAGCAC ACACACGCAC
GTAGTAGACA TCACTCGCAC CAGCCCTGCT GCCAAGAGCC CGTCGGCCCA ACTTATGGAG
CAAGTGGCTC AGCTTAAGTC CCTGAGTGAC ACCGTCGAGA AGCTCAAGGA TGAGGTCCTC
AAGGAGACAG TATCTCAGCG CCCTGGAGCC ACAGTACCCA CTGACTTTGC CACCTTCCCT
TCATCAGCCT TCCTCAGGGC CAAGGAGGAG CAGCAGGATG ACACAGTCTA CATGGGCAAA
GTGACCTTCT CATGTGCGGC TGGTTTTGGA CAGCGACACC GGCTGGTGCT GACCCAGGAG
CAGCTGCACC AGCTTCACAG TCGCCTCATC TCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001177766.1
CDS319..4134
Misc Feature(1)253..255(+)
Misc Feature(2)403..603(+)
Misc Feature(3)640..642(+)
Misc Feature(4)928..>1287(+)
Misc Feature(5)1033..1419(+)
Misc Feature(6)1051..1590(+)
Misc Feature(7)1237..1794(+)
Misc Feature(8)1873..2712(+)
Misc Feature(9)3214..3441(+)
Exon (1)1..351
Gene:DCTN1
Gene Synonym:
Exon (2)352..597
Gene:DCTN1
Gene Synonym:
Exon (3)598..676
Gene:DCTN1
Gene Synonym:
Exon (4)677..711
Gene:DCTN1
Gene Synonym:
Exon (5)712..729
Gene:DCTN1
Gene Synonym:
Exon (6)730..750
Gene:DCTN1
Gene Synonym:
Exon (7)751..942
Gene:DCTN1
Gene Synonym:
Exon (8)943..1140
Gene:DCTN1
Gene Synonym:
Exon (9)1141..1345
Gene:DCTN1
Gene Synonym:
Exon (10)1346..1424
Gene:DCTN1
Gene Synonym:
Exon (11)1425..1584
Gene:DCTN1
Gene Synonym:
Exon (12)1585..1689
Gene:DCTN1
Gene Synonym:
Exon (13)1690..1881
Gene:DCTN1
Gene Synonym:
Exon (14)1882..1998
Gene:DCTN1
Gene Synonym:
Exon (15)1999..2151
Gene:DCTN1
Gene Synonym:
Exon (16)2152..2312
Gene:DCTN1
Gene Synonym:
Exon (17)2313..2481
Gene:DCTN1
Gene Synonym:
Exon (18)2482..2550
Gene:DCTN1
Gene Synonym:
Exon (19)2551..2613
Gene:DCTN1
Gene Synonym:
Exon (20)2614..2763
Gene:DCTN1
Gene Synonym:
Exon (21)2764..2925
Gene:DCTN1
Gene Synonym:
Exon (22)2926..3057
Gene:DCTN1
Gene Synonym:
Exon (23)3058..3183
Gene:DCTN1
Gene Synonym:
Exon (24)3184..3326
Gene:DCTN1
Gene Synonym:
Exon (25)3327..3493
Gene:DCTN1
Gene Synonym:
Exon (26)3494..3508
Gene:DCTN1
Gene Synonym:
Exon (27)3509..3642
Gene:DCTN1
Gene Synonym:
Exon (28)3643..3826
Gene:DCTN1
Gene Synonym:
Exon (29)3827..3906
Gene:DCTN1
Gene Synonym:
Exon (30)3907..3996
Gene:DCTN1
Gene Synonym:
Exon (31)3997..4479
Gene:DCTN1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001190837
Organism Homo sapiens (human)
Definition Homo sapiens dynactin 1 (DCTN1), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001190837

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGGCACCGA CAGCCCGAAA GACCACAACT
CGGCGACCCA AGCCCACGCG CCCAGCCAGT ACTGGGGTGG CTGGGGCCAG TAGCTCCCTG
GGCCCCTCTG GCTCAGCGTC AGCAGGTGAG CTGAGCAGCA GTGAGCCCAG CACCCCGGCT
CAGACTCCGC TGGCAGCACC CATCATCCCC ACGCCGGTCC TCACCTCTCC TGGAGCAGTC
CCCCCGCTTC CTTCCCCATC CAAGGAGGAG GAGGGACTAA GGGCTCAGGT GCGGGACCTG
GAGGAGAAAC TAGAGACCCT GAGACTGAAA CGGGCAGAAG ACAAAGCAAA GCTAAAAGAG
CTGGAGAAAC ACAAAATCCA GCTGGAGCAG GTGCAGGAAT GGAAGAGCAA AATGCAGGAG
CAGCAGGCCG ACCTGCAGCG GCGCCTCAAG GAGGCGAGAA AGGAAGCCAA GGAGGCGCTG
GAGGCAAAGG AACGCTATAT GGAGGAGATG GCTGATACTG CTGATGCCAT TGAGATGGCC
ACTTTGGACA AGGAGATGGC TGAAGAGCGG GCTGAGTCCC TGCAGCAGGA GGTGGAGGCA
CTGAAGGAGC GGGTGGACGA GCTCACTACT GACTTAGAGA TCCTCAAGGC TGAGATTGAA
GAGAAGGGCT CAGATGGCGC TGCATCCAGT TATCAGCTCA AGCAGCTTGA GGAGCAGAAT
GCCCGCCTGA AGGATGCCCT GGTGAGGATG CGGGATCTTT CTTCCTCAGA GAAGCAGGAG
CATGTGAAGC TCCAGAAGCT CATGGAAAAG AAGAACCAAG AGCTGGAAGT TGTGAGGCAA
CAGCGGGAGC GTCTGCAGGA GGAGCTAAGC CAGGCAGAGA GCACCATTGA TGAGCTCAAG
GAGCAGGTGG ATGCTGCTCT GGGTGCTGAG GAGATGGTGG AGATGCTGAC AGATCGGAAC
CTGAATCTGG AAGAGAAAGT GCGCGAGTTG AGGGAGACTG TGGGAGACTT GGAAGCGATG
AATGAGATGA ACGATGAGCT GCAGGAGAAT GCACGTGAGA CAGAACTGGA GCTGCGGGAG
CAGCTGGACA TGGCAGGCGC GCGGGTTCGT GAGGCCCAGA AGCGTGTGGA GGCAGCCCAG
GAGACGGTTG CAGACTACCA GCAGACCATC AAGAAGTACC GCCAGCTGAC CGCCCATCTA
CAGGATGTGA ATCGGGAACT GACAAACCAG CAGGAAGCAT CTGTGGAGAG GCAACAGCAG
CCACCTCCAG AGACCTTTGA CTTCAAAATC AAGTTTGCTG AGACTAAGGC CCATGCCAAG
GCAATTGAGA TGGAATTGAG GCAGATGGAG GTGGCCCAGG CCAATCGACA CATGTCCCTG
CTGACAGCCT TCATGCCTGA CAGCTTCCTT CGGCCAGGTG GGGACCATGA CTGCGTTCTG
GTGCTGTTGC TCATGCCTCG TCTCATTTGC AAGGCAGAGC TGATCCGGAA GCAGGCCCAG
GAGAAGTTTG AACTAAGTGA GAACTGTTCA GAGCGGCCTG GGCTGCGAGG AGCTGCTGGG
GAGCAACTCA GCTTTGCTGC TGGACTGGTG TACTCGCTGA GCCTGCTGCA GGCCACGCTA
CACCGCTATG AGCATGCCCT CTCTCAGTGC AGTGTGGATG TGTATAAGAA AGTGGGCAGC
CTGTACCCTG AGATGAGTGC CCATGAGCGC TCCTTGGATT TCCTCATTGA ACTGCTGCAC
AAGGATCAGC TGGATGAGAC TGTCAATGTG GAGCCTCTCA CCAAGGCCAT CAAGTACTAT
CAGCATCTGT ACAGCATCCA CCTTGCCGAA CAGCCTGAGG ACTGTACTAT GCAGCTGGCT
GACCACATTA AGTTCACGCA GAGTGCTCTG GACTGCATGA GTGTGGAGGT AGGACGGCTG
CGTGCCTTCT TGCAGGGTGG GCAGGAGGCT ACAGATATTG CCCTCCTGCT CCGGGATCTG
GAAACTTCAT GCAGTGACAT CCGCCAGTTC TGCAAGAAGA TCCGAAGGCG AATGCCAGGG
ACAGATGCTC CTGGGATCCC AGCTGCACTG GCCTTTGGAC CACAGGTATC TGACACGCTC
CTAGACTGCA GGAAACACTT GACGTGGGTC GTGGCTGTGC TGCAGGAGGT GGCAGCTGCT
GCTGCCCAGC TCATTGCCCC ACTGGCAGAG AATGAGGGGC TACTTGTGGC TGCTCTGGAG
GAACTGGCTT TCAAAGCAAG CGAGCAGATC TATGGGACCC CCTCCAGCAG CCCCTATGAG
TGTCTGCGCC AGTCATGCAA CATCCTCATC AGTACCATGA ACAAGCTGGC CACAGCCATG
CAGGAGGGGG AGTATGATGC AGAGCGGCCC CCCAGCAAGC CTCCACCGGT TGAACTGCGG
GCTGCTGCCC TTCGTGCAGA GATCACAGAT GCTGAAGGCC TGGGTTTGAA GCTCGAAGAT
CGAGAGACAG TTATTAAGGA GTTGAAGAAG TCACTCAAGA TTAAGGGAGA GGAGCTAAGT
GAGGCCAATG TGCGGCTGAG CCTCCTGGAG AAGAAGTTGG ACAGTGCTGC CAAGGATGCA
GATGAGCGCA TCGAGAAAGT CCAGACTCGG CTGGAGGAGA CCCAGGCACT GCTGCGAAAG
AAGGAGAAAG AGTTTGAGGA GACAATGGAT GCACTCCAGG CTGACATCGA CCAGCTGGAG
GCAGAGAAGG CAGAACTAAA GCAGCGTCTG AACAGCCAGT CCAAACGCAC GATTGAGGGA
CTCCGGGGCC CTCCTCCTTC AGGCATTGCT ACTCTGGTCT CTGGCATTGC TGGTGAAGAA
CAGCAGCGAG GAGCCATCCC TGGGCAGGCT CCAGGGTCTG TGCCAGGCCC AGGGCTGGTG
AAGGACTCAC CACTGCTGCT TCAGCAGATC TCTGCCATGA GGCTGCACAT CTCCCAGCTC
CAGCATGAGA ACAGCATCCT CAAGGGAGCC CAGATGAAGG CATCCTTGGC ATCCCTGCCC
CCTCTGCATG TTGCAAAGCT ATCCCATGAG GGCCCTGGCA GTGAGTTACC AGCTGGAGCG
CTGTATCGTA AGACCAGCCA GCTGCTGGAG ACATTGAATC AATTGAGCAC ACACACGCAC
GTAGTAGACA TCACTCGCAC CAGCCCTGCT GCCAAGAGCC CGTCGGCCCA ACTTATGGAG
CAAGTGGCTC AGCTTAAGTC CCTGAGTGAC ACCGTCGAGA AGCTCAAGGA TGAGGTCCTC
AAGGAGACAG TATCTCAGCG CCCTGGAGCC ACAGTACCCA CTGACTTTGC CACCTTCCCT
TCATCAGCCT TCCTCAGGGC CAAGGAGGAG CAGCAGGATG ACACAGTCTA CATGGGCAAA
GTGACCTTCT CATGTGCGGC TGGTTTTGGA CAGCGACACC GGCTGGTGCT GACCCAGGAG
CAGCTGCACC AGCTTCACAG TCGCCTCATC TCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16796
Clone ID Related Accession (Same CDS sequence) NM_001135040
Accession Version NM_001135040.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3762bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dynactin subunit 1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA315227.1, AK314352.1, BC013978.2 and CA448857.1. On Jul 1, 2010 this sequence version replaced gi:205277391. Transcript Variant: This variant (3) has multiple differences in the 5' coding region and 5' UTR, compared to variant 4. These differences cause translation initiation from an upstream AUG and a protein (isoform 3) with a longer N-terminus containing a CAP-Gly domain, compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314352.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGCCCACGC GCCCAGCCAG TACTGGGGTG
GCTGGGGCCA GTAGCTCCCT GGGCCCCTCT GGCTCAGCGT CAGCAGGTGA GCTGAGCAGC
AGTGAGCCCA GCACCCCGGC TCAGACTCCG CTGGCAGCAC CCATCATCCC CACGCCGGTC
CTCACCTCTC CTGGAGCAGT CCCCCCGCTT CCTTCCCCAT CCAAGGAGGA GGAGGGACTA
AGGGCTCAGG TGCGGGACCT GGAGGAGAAA CTAGAGACCC TGAGACTGAA ACGGGCAGAA
GACAAAGCAA AGCTAAAAGA GCTGGAGAAA CACAAAATCC AGCTGGAGCA GGTGCAGGAA
TGGAAGAGCA AAATGCAGGA GCAGCAGGCC GACCTGCAGC GGCGCCTCAA GGAGGCGAGA
AAGGAAGCCA AGGAGGCGCT GGAGGCAAAG GAACGCTATA TGGAGGAGAT GGCTGATACT
GCTGATGCCA TTGAGATGGC CACTTTGGAC AAGGAGATGG CTGAAGAGCG GGCTGAGTCC
CTGCAGCAGG AGGTGGAGGC ACTGAAGGAG CGGGTGGACG AGCTCACTAC TGACTTAGAG
ATCCTCAAGG CTGAGATTGA AGAGAAGGGC TCAGATGGCG CTGCATCCAG TTATCAGCTC
AAGCAGCTTG AGGAGCAGAA TGCCCGCCTG AAGGATGCCC TGGTGAGGAT GCGGGATCTT
TCTTCCTCAG AGAAGCAGGA GCATGTGAAG CTCCAGAAGC TCATGGAAAA GAAGAACCAA
GAGCTGGAAG TTGTGAGGCA ACAGCGGGAG CGTCTGCAGG AGGAGCTAAG CCAGGCAGAG
AGCACCATTG ATGAGCTCAA GGAGCAGGTG GATGCTGCTC TGGGTGCTGA GGAGATGGTG
GAGATGCTGA CAGATCGGAA CCTGAATCTG GAAGAGAAAG TGCGCGAGTT GAGGGAGACT
GTGGGAGACT TGGAAGCGAT GAATGAGATG AACGATGAGC TGCAGGAGAA TGCACGTGAG
ACAGAACTGG AGCTGCGGGA GCAGCTGGAC ATGGCAGGCG CGCGGGTTCG TGAGGCCCAG
AAGCGTGTGG AGGCAGCCCA GGAGACGGTT GCAGACTACC AGCAGACCAT CAAGAAGTAC
CGCCAGCTGA CCGCCCATCT ACAGGATGTG AATCGGGAAC TGACAAACCA GCAGGAAGCA
TCTGTGGAGA GGCAACAGCA GCCACCTCCA GAGACCTTTG ACTTCAAAAT CAAGTTTGCT
GAGACTAAGG CCCATGCCAA GGCAATTGAG ATGGAATTGA GGCAGATGGA GGTGGCCCAG
GCCAATCGAC ACATGTCCCT GCTGACAGCC TTCATGCCTG ACAGCTTCCT TCGGCCAGGT
GGGGACCATG ACTGCGTTCT GGTGCTGTTG CTCATGCCTC GTCTCATTTG CAAGGCAGAG
CTGATCCGGA AGCAGGCCCA GGAGAAGTTT GAACTAAGTG AGAACTGTTC AGAGCGGCCT
GGGCTGCGAG GAGCTGCTGG GGAGCAACTC AGCTTTGCTG CTGGACTGGT GTACTCGCTG
AGCCTGCTGC AGGCCACGCT ACACCGCTAT GAGCATGCCC TCTCTCAGTG CAGTGTGGAT
GTGTATAAGA AAGTGGGCAG CCTGTACCCT GAGATGAGTG CCCATGAGCG CTCCTTGGAT
TTCCTCATTG AACTGCTGCA CAAGGATCAG CTGGATGAGA CTGTCAATGT GGAGCCTCTC
ACCAAGGCCA TCAAGTACTA TCAGCATCTG TACAGCATCC ACCTTGCCGA ACAGCCTGAG
GACTGTACTA TGCAGCTGGC TGACCACATT AAGTTCACGC AGAGTGCTCT GGACTGCATG
AGTGTGGAGG TAGGACGGCT GCGTGCCTTC TTGCAGGGTG GGCAGGAGGC TACAGATATT
GCCCTCCTGC TCCGGGATCT GGAAACTTCA TGCAGTGACA TCCGCCAGTT CTGCAAGAAG
ATCCGAAGGC GAATGCCAGG GACAGATGCT CCTGGGATCC CAGCTGCACT GGCCTTTGGA
CCACAGGTAT CTGACACGCT CCTAGACTGC AGGAAACACT TGACGTGGGT CGTGGCTGTG
CTGCAGGAGG TGGCAGCTGC TGCTGCCCAG CTCATTGCCC CACTGGCAGA GAATGAGGGG
CTACTTGTGG CTGCTCTGGA GGAACTGGCT TTCAAAGCAA GCGAGCAGAT CTATGGGACC
CCCTCCAGCA GCCCCTATGA GTGTCTGCGC CAGTCATGCA ACATCCTCAT CAGTACCATG
AACAAGCTGG CCACAGCCAT GCAGGAGGGG GAGTATGATG CAGAGCGGCC CCCCAGCAAG
CCTCCACCGG TTGAACTGCG GGCTGCTGCC CTTCGTGCAG AGATCACAGA TGCTGAAGGC
CTGGGTTTGA AGCTCGAAGA TCGAGAGACA GTTATTAAGG AGTTGAAGAA GTCACTCAAG
ATTAAGGGAG AGGAGCTAAG TGAGGCCAAT GTGCGGCTGA GCCTCCTGGA GAAGAAGTTG
GACAGTGCTG CCAAGGATGC AGATGAGCGC ATCGAGAAAG TCCAGACTCG GCTGGAGGAG
ACCCAGGCAC TGCTGCGAAA GAAGGAGAAA GAGTTTGAGG AGACAATGGA TGCACTCCAG
GCTGACATCG ACCAGCTGGA GGCAGAGAAG GCAGAACTAA AGCAGCGTCT GAACAGCCAG
TCCAAACGCA CGATTGAGGG ACTCCGGGGC CCTCCTCCTT CAGGCATTGC TACTCTGGTC
TCTGGCATTG CTGGTGGAGC CATCCCTGGG CAGGCTCCAG GGTCTGTGCC AGGCCCAGGG
CTGGTGAAGG ACTCACCACT GCTGCTTCAG CAGATCTCTG CCATGAGGCT GCACATCTCC
CAGCTCCAGC ATGAGAACAG CATCCTCAAG GGAGCCCAGA TGAAGGCATC CTTGGCATCC
CTGCCCCCTC TGCATGTTGC AAAGCTATCC CATGAGGGCC CTGGCAGTGA GTTACCAGCT
GGAGCGCTGT ATCGTAAGAC CAGCCAGCTG CTGGAGACAT TGAATCAATT GAGCACACAC
ACGCACGTAG TAGACATCAC TCGCACCAGC CCTGCTGCCA AGAGCCCGTC GGCCCAACTT
ATGGAGCAAG TGGCTCAGCT TAAGTCCCTG AGTGACACCG TCGAGAAGCT CAAGGATGAG
GTCCTCAAGG AGACAGTATC TCAGCGCCCT GGAGCCACAG TACCCACTGA CTTTGCCACC
TTCCCTTCAT CAGCCTTCCT CAGGGCCAAG GAGGAGCAGC AGGATGACAC AGTCTACATG
GGCAAAGTGA CCTTCTCATG TGCGGCTGGT TTTGGACAGC GACACCGGCT GGTGCTGACC
CAGGAGCAGC TGCACCAGCT TCACAGTCGC CTCATCTCCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001128512.1
CDS319..4080
Misc Feature(1)253..255(+)
Misc Feature(2)403..603(+)
Misc Feature(3)640..642(+)
Misc Feature(4)889..>1248(+)
Misc Feature(5)994..1380(+)
Misc Feature(6)1012..1551(+)
Misc Feature(7)1198..1755(+)
Misc Feature(8)1834..2673(+)
Misc Feature(9)3175..3402(+)
Exon (1)1..351
Gene:DCTN1
Gene Synonym:
Exon (2)352..597
Gene:DCTN1
Gene Synonym:
Exon (3)598..676
Gene:DCTN1
Gene Synonym:
Exon (4)677..711
Gene:DCTN1
Gene Synonym:
Exon (5)712..903
Gene:DCTN1
Gene Synonym:
Exon (6)904..1101
Gene:DCTN1
Gene Synonym:
Exon (7)1102..1306
Gene:DCTN1
Gene Synonym:
Exon (8)1307..1385
Gene:DCTN1
Gene Synonym:
Exon (9)1386..1545
Gene:DCTN1
Gene Synonym:
Exon (10)1546..1650
Gene:DCTN1
Gene Synonym:
Exon (11)1651..1842
Gene:DCTN1
Gene Synonym:
Exon (12)1843..1959
Gene:DCTN1
Gene Synonym:
Exon (13)1960..2112
Gene:DCTN1
Gene Synonym:
Exon (14)2113..2273
Gene:DCTN1
Gene Synonym:
Exon (15)2274..2442
Gene:DCTN1
Gene Synonym:
Exon (16)2443..2511
Gene:DCTN1
Gene Synonym:
Exon (17)2512..2574
Gene:DCTN1
Gene Synonym:
Exon (18)2575..2724
Gene:DCTN1
Gene Synonym:
Exon (19)2725..2886
Gene:DCTN1
Gene Synonym:
Exon (20)2887..3018
Gene:DCTN1
Gene Synonym:
Exon (21)3019..3144
Gene:DCTN1
Gene Synonym:
Exon (22)3145..3287
Gene:DCTN1
Gene Synonym:
Exon (23)3288..3454
Gene:DCTN1
Gene Synonym:
Exon (24)3455..3588
Gene:DCTN1
Gene Synonym:
Exon (25)3589..3772
Gene:DCTN1
Gene Synonym:
Exon (26)3773..3852
Gene:DCTN1
Gene Synonym:
Exon (27)3853..3942
Gene:DCTN1
Gene Synonym:
Exon (28)3943..4425
Gene:DCTN1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001135040
Organism Homo sapiens (human)
Definition Homo sapiens dynactin 1 (DCTN1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001135040

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGCCCACGC GCCCAGCCAG TACTGGGGTG
GCTGGGGCCA GTAGCTCCCT GGGCCCCTCT GGCTCAGCGT CAGCAGGTGA GCTGAGCAGC
AGTGAGCCCA GCACCCCGGC TCAGACTCCG CTGGCAGCAC CCATCATCCC CACGCCGGTC
CTCACCTCTC CTGGAGCAGT CCCCCCGCTT CCTTCCCCAT CCAAGGAGGA GGAGGGACTA
AGGGCTCAGG TGCGGGACCT GGAGGAGAAA CTAGAGACCC TGAGACTGAA ACGGGCAGAA
GACAAAGCAA AGCTAAAAGA GCTGGAGAAA CACAAAATCC AGCTGGAGCA GGTGCAGGAA
TGGAAGAGCA AAATGCAGGA GCAGCAGGCC GACCTGCAGC GGCGCCTCAA GGAGGCGAGA
AAGGAAGCCA AGGAGGCGCT GGAGGCAAAG GAACGCTATA TGGAGGAGAT GGCTGATACT
GCTGATGCCA TTGAGATGGC CACTTTGGAC AAGGAGATGG CTGAAGAGCG GGCTGAGTCC
CTGCAGCAGG AGGTGGAGGC ACTGAAGGAG CGGGTGGACG AGCTCACTAC TGACTTAGAG
ATCCTCAAGG CTGAGATTGA AGAGAAGGGC TCAGATGGCG CTGCATCCAG TTATCAGCTC
AAGCAGCTTG AGGAGCAGAA TGCCCGCCTG AAGGATGCCC TGGTGAGGAT GCGGGATCTT
TCTTCCTCAG AGAAGCAGGA GCATGTGAAG CTCCAGAAGC TCATGGAAAA GAAGAACCAA
GAGCTGGAAG TTGTGAGGCA ACAGCGGGAG CGTCTGCAGG AGGAGCTAAG CCAGGCAGAG
AGCACCATTG ATGAGCTCAA GGAGCAGGTG GATGCTGCTC TGGGTGCTGA GGAGATGGTG
GAGATGCTGA CAGATCGGAA CCTGAATCTG GAAGAGAAAG TGCGCGAGTT GAGGGAGACT
GTGGGAGACT TGGAAGCGAT GAATGAGATG AACGATGAGC TGCAGGAGAA TGCACGTGAG
ACAGAACTGG AGCTGCGGGA GCAGCTGGAC ATGGCAGGCG CGCGGGTTCG TGAGGCCCAG
AAGCGTGTGG AGGCAGCCCA GGAGACGGTT GCAGACTACC AGCAGACCAT CAAGAAGTAC
CGCCAGCTGA CCGCCCATCT ACAGGATGTG AATCGGGAAC TGACAAACCA GCAGGAAGCA
TCTGTGGAGA GGCAACAGCA GCCACCTCCA GAGACCTTTG ACTTCAAAAT CAAGTTTGCT
GAGACTAAGG CCCATGCCAA GGCAATTGAG ATGGAATTGA GGCAGATGGA GGTGGCCCAG
GCCAATCGAC ACATGTCCCT GCTGACAGCC TTCATGCCTG ACAGCTTCCT TCGGCCAGGT
GGGGACCATG ACTGCGTTCT GGTGCTGTTG CTCATGCCTC GTCTCATTTG CAAGGCAGAG
CTGATCCGGA AGCAGGCCCA GGAGAAGTTT GAACTAAGTG AGAACTGTTC AGAGCGGCCT
GGGCTGCGAG GAGCTGCTGG GGAGCAACTC AGCTTTGCTG CTGGACTGGT GTACTCGCTG
AGCCTGCTGC AGGCCACGCT ACACCGCTAT GAGCATGCCC TCTCTCAGTG CAGTGTGGAT
GTGTATAAGA AAGTGGGCAG CCTGTACCCT GAGATGAGTG CCCATGAGCG CTCCTTGGAT
TTCCTCATTG AACTGCTGCA CAAGGATCAG CTGGATGAGA CTGTCAATGT GGAGCCTCTC
ACCAAGGCCA TCAAGTACTA TCAGCATCTG TACAGCATCC ACCTTGCCGA ACAGCCTGAG
GACTGTACTA TGCAGCTGGC TGACCACATT AAGTTCACGC AGAGTGCTCT GGACTGCATG
AGTGTGGAGG TAGGACGGCT GCGTGCCTTC TTGCAGGGTG GGCAGGAGGC TACAGATATT
GCCCTCCTGC TCCGGGATCT GGAAACTTCA TGCAGTGACA TCCGCCAGTT CTGCAAGAAG
ATCCGAAGGC GAATGCCAGG GACAGATGCT CCTGGGATCC CAGCTGCACT GGCCTTTGGA
CCACAGGTAT CTGACACGCT CCTAGACTGC AGGAAACACT TGACGTGGGT CGTGGCTGTG
CTGCAGGAGG TGGCAGCTGC TGCTGCCCAG CTCATTGCCC CACTGGCAGA GAATGAGGGG
CTACTTGTGG CTGCTCTGGA GGAACTGGCT TTCAAAGCAA GCGAGCAGAT CTATGGGACC
CCCTCCAGCA GCCCCTATGA GTGTCTGCGC CAGTCATGCA ACATCCTCAT CAGTACCATG
AACAAGCTGG CCACAGCCAT GCAGGAGGGG GAGTATGATG CAGAGCGGCC CCCCAGCAAG
CCTCCACCGG TTGAACTGCG GGCTGCTGCC CTTCGTGCAG AGATCACAGA TGCTGAAGGC
CTGGGTTTGA AGCTCGAAGA TCGAGAGACA GTTATTAAGG AGTTGAAGAA GTCACTCAAG
ATTAAGGGAG AGGAGCTAAG TGAGGCCAAT GTGCGGCTGA GCCTCCTGGA GAAGAAGTTG
GACAGTGCTG CCAAGGATGC AGATGAGCGC ATCGAGAAAG TCCAGACTCG GCTGGAGGAG
ACCCAGGCAC TGCTGCGAAA GAAGGAGAAA GAGTTTGAGG AGACAATGGA TGCACTCCAG
GCTGACATCG ACCAGCTGGA GGCAGAGAAG GCAGAACTAA AGCAGCGTCT GAACAGCCAG
TCCAAACGCA CGATTGAGGG ACTCCGGGGC CCTCCTCCTT CAGGCATTGC TACTCTGGTC
TCTGGCATTG CTGGTGGAGC CATCCCTGGG CAGGCTCCAG GGTCTGTGCC AGGCCCAGGG
CTGGTGAAGG ACTCACCACT GCTGCTTCAG CAGATCTCTG CCATGAGGCT GCACATCTCC
CAGCTCCAGC ATGAGAACAG CATCCTCAAG GGAGCCCAGA TGAAGGCATC CTTGGCATCC
CTGCCCCCTC TGCATGTTGC AAAGCTATCC CATGAGGGCC CTGGCAGTGA GTTACCAGCT
GGAGCGCTGT ATCGTAAGAC CAGCCAGCTG CTGGAGACAT TGAATCAATT GAGCACACAC
ACGCACGTAG TAGACATCAC TCGCACCAGC CCTGCTGCCA AGAGCCCGTC GGCCCAACTT
ATGGAGCAAG TGGCTCAGCT TAAGTCCCTG AGTGACACCG TCGAGAAGCT CAAGGATGAG
GTCCTCAAGG AGACAGTATC TCAGCGCCCT GGAGCCACAG TACCCACTGA CTTTGCCACC
TTCCCTTCAT CAGCCTTCCT CAGGGCCAAG GAGGAGCAGC AGGATGACAC AGTCTACATG
GGCAAAGTGA CCTTCTCATG TGCGGCTGGT TTTGGACAGC GACACCGGCT GGTGCTGACC
CAGGAGCAGC TGCACCAGCT TCACAGTCGC CTCATCTCCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16697
Clone ID Related Accession (Same CDS sequence) NM_001135041
Accession Version NM_001135041.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3420bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dynactin subunit 1 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA111497.1, BC071583.1, BC013978.2 and CA448857.1. On Jul 1, 2010 this sequence version replaced gi:205277395. Transcript Variant: This variant (4) represents the shortest transcript and it encodes the shortest protein (isoform 4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071583.1, AK315014.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGATGAGAC AGGCACCGAC AGCCCGAAAG ACCACAACTC GGCGACCCAA GCCCACGCGC 
CCAGCCAGTA CTGGGGTGGC TGGGGCCAGT AGCTCCCTGG GCCCCTCTGG CTCAGCGTCA
GCAGGTGAGC TGAGCAGCAG TGAGCCCAGC ACCCCGGCTC AGACTCCGCT GGCAGCACCC
ATCATCCCCA CGCCGGTCCT CACCTCTCCT GGAGCAGTCC CCCCGCTTCC TTCCCCATCC
AAGGAGGAGG AGGGACTAAG GGCTCAGGTG CGGGACCTGG AGGAGAAACT AGAGACCCTG
AGACTGAAAC GGGCAGAAGA CAAAGCAAAG CTAAAAGAGC TGGAGAAACA CAAAATCCAG
CTGGAGCAGG TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCCGA CCTGCAGCGG
CGCCTCAAGG AGGCGAGAAA GGAAGCCAAG GAGGCGCTGG AGGCAAAGGA ACGCTATATG
GAGGAGATGG CTGATACTGC TGATGCCATT GAGATGGCCA CTTTGGACAA GGAGATGGCT
GAAGAGCGGG CTGAGTCCCT GCAGCAGGAG GTGGAGGCAC TGAAGGAGCG GGTGGACGAG
CTCACTACTG ACTTAGAGAT CCTCAAGGCT GAGATTGAAG AGAAGGGCTC AGATGGCGCT
GCATCCAGTT ATCAGCTCAA GCAGCTTGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG
GTGAGGATGC GGGATCTTTC TTCCTCAGAG AAGCAGGAGC ATGTGAAGCT CCAGAAGCTC
ATGGAAAAGA AGAACCAAGA GCTGGAAGTT GTGAGGCAAC AGCGGGAGCG TCTGCAGGAG
GAGCTAAGCC AGGCAGAGAG CACCATTGAT GAGCTCAAGG AGCAGGTGGA TGCTGCTCTG
GGTGCTGAGG AGATGGTGGA GATGCTGACA GATCGGAACC TGAATCTGGA AGAGAAAGTG
CGCGAGTTGA GGGAGACTGT GGGAGACTTG GAAGCGATGA ATGAGATGAA CGATGAGCTG
CAGGAGAATG CACGTGAGAC AGAACTGGAG CTGCGGGAGC AGCTGGACAT GGCAGGCGCG
CGGGTTCGTG AGGCCCAGAA GCGTGTGGAG GCAGCCCAGG AGACGGTTGC AGACTACCAG
CAGACCATCA AGAAGTACCG CCAGCTGACC GCCCATCTAC AGGATGTGAA TCGGGAACTG
ACAAACCAGC AGGAAGCATC TGTGGAGAGG CAACAGCAGC CACCTCCAGA GACCTTTGAC
TTCAAAATCA AGTTTGCTGA GACTAAGGCC CATGCCAAGG CAATTGAGAT GGAATTGAGG
CAGATGGAGG TGGCCCAGGC CAATCGACAC ATGTCCCTGC TGACAGCCTT CATGCCTGAC
AGCTTCCTTC GGCCAGGTGG GGACCATGAC TGCGTTCTGG TGCTGTTGCT CATGCCTCGT
CTCATTTGCA AGGCAGAGCT GATCCGGAAG CAGGCCCAGG AGAAGTTTGA ACTAAGTGAG
AACTGTTCAG AGCGGCCTGG GCTGCGAGGA GCTGCTGGGG AGCAACTCAG CTTTGCTGCT
GGACTGGTGT ACTCGCTGAG CCTGCTGCAG GCCACGCTAC ACCGCTATGA GCATGCCCTC
TCTCAGTGCA GTGTGGATGT GTATAAGAAA GTGGGCAGCC TGTACCCTGA GATGAGTGCC
CATGAGCGCT CCTTGGATTT CCTCATTGAA CTGCTGCACA AGGATCAGCT GGATGAGACT
GTCAATGTGG AGCCTCTCAC CAAGGCCATC AAGTACTATC AGCATCTGTA CAGCATCCAC
CTTGCCGAAC AGCCTGAGGA CTGTACTATG CAGCTGGCTG ACCACATTAA GTTCACGCAG
AGTGCTCTGG ACTGCATGAG TGTGGAGGTA GGACGGCTGC GTGCCTTCTT GCAGGGTGGG
CAGGAGGCTA CAGATATTGC CCTCCTGCTC CGGGATCTGG AAACTTCATG CAGTGACATC
CGCCAGTTCT GCAAGAAGAT CCGAAGGCGA ATGCCAGGGA CAGATGCTCC TGGGATCCCA
GCTGCACTGG CCTTTGGACC ACAGGTATCT GACACGCTCC TAGACTGCAG GAAACACTTG
ACGTGGGTCG TGGCTGTGCT GCAGGAGGTG GCAGCTGCTG CTGCCCAGCT CATTGCCCCA
CTGGCAGAGA ATGAGGGGCT ACTTGTGGCT GCTCTGGAGG AACTGGCTTT CAAAGCAAGC
GAGCAGATCT ATGGGACCCC CTCCAGCAGC CCCTATGAGT GTCTGCGCCA GTCATGCAAC
ATCCTCATCA GTACCATGAA CAAGCTGGCC ACAGCCATGC AGGAGGGGGA GTATGATGCA
GAGCGGCCCC CCAGCAAGCC TCCACCGGTT GAACTGCGGG CTGCTGCCCT TCGTGCAGAG
ATCACAGATG CTGAAGGCCT GGGTTTGAAG CTCGAAGATC GAGAGACAGT TATTAAGGAG
TTGAAGAAGT CACTCAAGAT TAAGGGAGAG GAGCTAAGTG AGGCCAATGT GCGGCTGAGC
CTCCTGGAGA AGAAGTTGGA CAGTGCTGCC AAGGATGCAG ATGAGCGCAT CGAGAAAGTC
CAGACTCGGC TGGAGGAGAC CCAGGCACTG CTGCGAAAGA AGGAGAAAGA GTTTGAGGAG
ACAATGGATG CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAACTAAAG
CAGCGTCTGA ACAGCCAGTC CAAACGCACG ATTGAGGGAC TCCGGGGCCC TCCTCCTTCA
GGCATTGCTA CTCTGGTCTC TGGCATTGCT GGTGGAGCCA TCCCTGGGCA GGCTCCAGGG
TCTGTGCCAG GCCCAGGGCT GGTGAAGGAC TCACCACTGC TGCTTCAGCA GATCTCTGCC
ATGAGGCTGC ACATCTCCCA GCTCCAGCAT GAGAACAGCA TCCTCAAGGG AGCCCAGATG
AAGGCATCCT TGGCATCCCT GCCCCCTCTG CATGTTGCAA AGCTATCCCA TGAGGGCCCT
GGCAGTGAGT TACCAGCTGG AGCGCTGTAT CGTAAGACCA GCCAGCTGCT GGAGACATTG
AATCAATTGA GCACACACAC GCACGTAGTA GACATCACTC GCACCAGCCC TGCTGCCAAG
AGCCCGTCGG CCCAACTTAT GGAGCAAGTG GCTCAGCTTA AGTCCCTGAG TGACACCGTC
GAGAAGCTCA AGGATGAGGT CCTCAAGGAG ACAGTATCTC AGCGCCCTGG AGCCACAGTA
CCCACTGACT TTGCCACCTT CCCTTCATCA GCCTTCCTCA GGGCCAAGGA GGAGCAGCAG
GATGACACAG TCTACATGGG CAAAGTGACC TTCTCATGTG CGGCTGGTTT TGGACAGCGA
CACCGGCTGG TGCTGACCCA GGAGCAGCTG CACCAGCTTC ACAGTCGCCT CATCTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001128513.1
CDS384..3803
Misc Feature(1)240..242(+)
Misc Feature(2)612..>971(+)
Misc Feature(3)717..1103(+)
Misc Feature(4)735..1274(+)
Misc Feature(5)921..1478(+)
Misc Feature(6)1557..2396(+)
Misc Feature(7)2898..3125(+)
Exon (1)1..413
Gene:DCTN1
Gene Synonym:
Exon (2)414..434
Gene:DCTN1
Gene Synonym:
Exon (3)435..626
Gene:DCTN1
Gene Synonym:
Exon (4)627..824
Gene:DCTN1
Gene Synonym:
Exon (5)825..1029
Gene:DCTN1
Gene Synonym:
Exon (6)1030..1108
Gene:DCTN1
Gene Synonym:
Exon (7)1109..1268
Gene:DCTN1
Gene Synonym:
Exon (8)1269..1373
Gene:DCTN1
Gene Synonym:
Exon (9)1374..1565
Gene:DCTN1
Gene Synonym:
Exon (10)1566..1682
Gene:DCTN1
Gene Synonym:
Exon (11)1683..1835
Gene:DCTN1
Gene Synonym:
Exon (12)1836..1996
Gene:DCTN1
Gene Synonym:
Exon (13)1997..2165
Gene:DCTN1
Gene Synonym:
Exon (14)2166..2234
Gene:DCTN1
Gene Synonym:
Exon (15)2235..2297
Gene:DCTN1
Gene Synonym:
Exon (16)2298..2447
Gene:DCTN1
Gene Synonym:
Exon (17)2448..2609
Gene:DCTN1
Gene Synonym:
Exon (18)2610..2741
Gene:DCTN1
Gene Synonym:
Exon (19)2742..2867
Gene:DCTN1
Gene Synonym:
Exon (20)2868..3010
Gene:DCTN1
Gene Synonym:
Exon (21)3011..3177
Gene:DCTN1
Gene Synonym:
Exon (22)3178..3311
Gene:DCTN1
Gene Synonym:
Exon (23)3312..3495
Gene:DCTN1
Gene Synonym:
Exon (24)3496..3575
Gene:DCTN1
Gene Synonym:
Exon (25)3576..3665
Gene:DCTN1
Gene Synonym:
Exon (26)3666..4148
Gene:DCTN1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001135041
Organism Homo sapiens (human)
Definition Homo sapiens dynactin 1 (DCTN1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001135041

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGATGAGAC AGGCACCGAC AGCCCGAAAG ACCACAACTC GGCGACCCAA GCCCACGCGC 
CCAGCCAGTA CTGGGGTGGC TGGGGCCAGT AGCTCCCTGG GCCCCTCTGG CTCAGCGTCA
GCAGGTGAGC TGAGCAGCAG TGAGCCCAGC ACCCCGGCTC AGACTCCGCT GGCAGCACCC
ATCATCCCCA CGCCGGTCCT CACCTCTCCT GGAGCAGTCC CCCCGCTTCC TTCCCCATCC
AAGGAGGAGG AGGGACTAAG GGCTCAGGTG CGGGACCTGG AGGAGAAACT AGAGACCCTG
AGACTGAAAC GGGCAGAAGA CAAAGCAAAG CTAAAAGAGC TGGAGAAACA CAAAATCCAG
CTGGAGCAGG TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCCGA CCTGCAGCGG
CGCCTCAAGG AGGCGAGAAA GGAAGCCAAG GAGGCGCTGG AGGCAAAGGA ACGCTATATG
GAGGAGATGG CTGATACTGC TGATGCCATT GAGATGGCCA CTTTGGACAA GGAGATGGCT
GAAGAGCGGG CTGAGTCCCT GCAGCAGGAG GTGGAGGCAC TGAAGGAGCG GGTGGACGAG
CTCACTACTG ACTTAGAGAT CCTCAAGGCT GAGATTGAAG AGAAGGGCTC AGATGGCGCT
GCATCCAGTT ATCAGCTCAA GCAGCTTGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG
GTGAGGATGC GGGATCTTTC TTCCTCAGAG AAGCAGGAGC ATGTGAAGCT CCAGAAGCTC
ATGGAAAAGA AGAACCAAGA GCTGGAAGTT GTGAGGCAAC AGCGGGAGCG TCTGCAGGAG
GAGCTAAGCC AGGCAGAGAG CACCATTGAT GAGCTCAAGG AGCAGGTGGA TGCTGCTCTG
GGTGCTGAGG AGATGGTGGA GATGCTGACA GATCGGAACC TGAATCTGGA AGAGAAAGTG
CGCGAGTTGA GGGAGACTGT GGGAGACTTG GAAGCGATGA ATGAGATGAA CGATGAGCTG
CAGGAGAATG CACGTGAGAC AGAACTGGAG CTGCGGGAGC AGCTGGACAT GGCAGGCGCG
CGGGTTCGTG AGGCCCAGAA GCGTGTGGAG GCAGCCCAGG AGACGGTTGC AGACTACCAG
CAGACCATCA AGAAGTACCG CCAGCTGACC GCCCATCTAC AGGATGTGAA TCGGGAACTG
ACAAACCAGC AGGAAGCATC TGTGGAGAGG CAACAGCAGC CACCTCCAGA GACCTTTGAC
TTCAAAATCA AGTTTGCTGA GACTAAGGCC CATGCCAAGG CAATTGAGAT GGAATTGAGG
CAGATGGAGG TGGCCCAGGC CAATCGACAC ATGTCCCTGC TGACAGCCTT CATGCCTGAC
AGCTTCCTTC GGCCAGGTGG GGACCATGAC TGCGTTCTGG TGCTGTTGCT CATGCCTCGT
CTCATTTGCA AGGCAGAGCT GATCCGGAAG CAGGCCCAGG AGAAGTTTGA ACTAAGTGAG
AACTGTTCAG AGCGGCCTGG GCTGCGAGGA GCTGCTGGGG AGCAACTCAG CTTTGCTGCT
GGACTGGTGT ACTCGCTGAG CCTGCTGCAG GCCACGCTAC ACCGCTATGA GCATGCCCTC
TCTCAGTGCA GTGTGGATGT GTATAAGAAA GTGGGCAGCC TGTACCCTGA GATGAGTGCC
CATGAGCGCT CCTTGGATTT CCTCATTGAA CTGCTGCACA AGGATCAGCT GGATGAGACT
GTCAATGTGG AGCCTCTCAC CAAGGCCATC AAGTACTATC AGCATCTGTA CAGCATCCAC
CTTGCCGAAC AGCCTGAGGA CTGTACTATG CAGCTGGCTG ACCACATTAA GTTCACGCAG
AGTGCTCTGG ACTGCATGAG TGTGGAGGTA GGACGGCTGC GTGCCTTCTT GCAGGGTGGG
CAGGAGGCTA CAGATATTGC CCTCCTGCTC CGGGATCTGG AAACTTCATG CAGTGACATC
CGCCAGTTCT GCAAGAAGAT CCGAAGGCGA ATGCCAGGGA CAGATGCTCC TGGGATCCCA
GCTGCACTGG CCTTTGGACC ACAGGTATCT GACACGCTCC TAGACTGCAG GAAACACTTG
ACGTGGGTCG TGGCTGTGCT GCAGGAGGTG GCAGCTGCTG CTGCCCAGCT CATTGCCCCA
CTGGCAGAGA ATGAGGGGCT ACTTGTGGCT GCTCTGGAGG AACTGGCTTT CAAAGCAAGC
GAGCAGATCT ATGGGACCCC CTCCAGCAGC CCCTATGAGT GTCTGCGCCA GTCATGCAAC
ATCCTCATCA GTACCATGAA CAAGCTGGCC ACAGCCATGC AGGAGGGGGA GTATGATGCA
GAGCGGCCCC CCAGCAAGCC TCCACCGGTT GAACTGCGGG CTGCTGCCCT TCGTGCAGAG
ATCACAGATG CTGAAGGCCT GGGTTTGAAG CTCGAAGATC GAGAGACAGT TATTAAGGAG
TTGAAGAAGT CACTCAAGAT TAAGGGAGAG GAGCTAAGTG AGGCCAATGT GCGGCTGAGC
CTCCTGGAGA AGAAGTTGGA CAGTGCTGCC AAGGATGCAG ATGAGCGCAT CGAGAAAGTC
CAGACTCGGC TGGAGGAGAC CCAGGCACTG CTGCGAAAGA AGGAGAAAGA GTTTGAGGAG
ACAATGGATG CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAACTAAAG
CAGCGTCTGA ACAGCCAGTC CAAACGCACG ATTGAGGGAC TCCGGGGCCC TCCTCCTTCA
GGCATTGCTA CTCTGGTCTC TGGCATTGCT GGTGGAGCCA TCCCTGGGCA GGCTCCAGGG
TCTGTGCCAG GCCCAGGGCT GGTGAAGGAC TCACCACTGC TGCTTCAGCA GATCTCTGCC
ATGAGGCTGC ACATCTCCCA GCTCCAGCAT GAGAACAGCA TCCTCAAGGG AGCCCAGATG
AAGGCATCCT TGGCATCCCT GCCCCCTCTG CATGTTGCAA AGCTATCCCA TGAGGGCCCT
GGCAGTGAGT TACCAGCTGG AGCGCTGTAT CGTAAGACCA GCCAGCTGCT GGAGACATTG
AATCAATTGA GCACACACAC GCACGTAGTA GACATCACTC GCACCAGCCC TGCTGCCAAG
AGCCCGTCGG CCCAACTTAT GGAGCAAGTG GCTCAGCTTA AGTCCCTGAG TGACACCGTC
GAGAAGCTCA AGGATGAGGT CCTCAAGGAG ACAGTATCTC AGCGCCCTGG AGCCACAGTA
CCCACTGACT TTGCCACCTT CCCTTCATCA GCCTTCCTCA GGGCCAAGGA GGAGCAGCAG
GATGACACAG TCTACATGGG CAAAGTGACC TTCTCATGTG CGGCTGGTTT TGGACAGCGA
CACCGGCTGG TGCTGACCCA GGAGCAGCTG CACCAGCTTC ACAGTCGCCT CATCTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18647
Clone ID Related Accession (Same CDS sequence) NM_004082
Accession Version NM_004082.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dynactin subunit 1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA315227.1, BX640799.1, BC013978.2, X98801.1 and CA448857.1. This sequence is a reference standard in the RefSeqGene project. On Jul 1, 2010 this sequence version replaced gi:205277389. Transcript Variant: This variant (1) has multiple differences in the 5' UTR, 5' coding region and 3' coding region, compared to variant 4. These differences cause translation initiation from an upstream AUG and a protein (isoform 1) with a longer N-terminus containing a CAP-Gly domain and a longer C-terminus, compared to isoform 4. Isoform 1 is also called p150. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640799.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGCGGGGAC TGAAGCCTAA GAAGGCACCG
ACAGCCCGAA AGACCACAAC TCGGCGACCC AAGCCCACGC GCCCAGCCAG TACTGGGGTG
GCTGGGGCCA GTAGCTCCCT GGGCCCCTCT GGCTCAGCGT CAGCAGGTGA GCTGAGCAGC
AGTGAGCCCA GCACCCCGGC TCAGACTCCG CTGGCAGCAC CCATCATCCC CACGCCGGTC
CTCACCTCTC CTGGAGCAGT CCCCCCGCTT CCTTCCCCAT CCAAGGAGGA GGAGGGACTA
AGGGCTCAGG TGCGGGACCT GGAGGAGAAA CTAGAGACCC TGAGACTGAA ACGGGCAGAA
GACAAAGCAA AGCTAAAAGA GCTGGAGAAA CACAAAATCC AGCTGGAGCA GGTGCAGGAA
TGGAAGAGCA AAATGCAGGA GCAGCAGGCC GACCTGCAGC GGCGCCTCAA GGAGGCGAGA
AAGGAAGCCA AGGAGGCGCT GGAGGCAAAG GAACGCTATA TGGAGGAGAT GGCTGATACT
GCTGATGCCA TTGAGATGGC CACTTTGGAC AAGGAGATGG CTGAAGAGCG GGCTGAGTCC
CTGCAGCAGG AGGTGGAGGC ACTGAAGGAG CGGGTGGACG AGCTCACTAC TGACTTAGAG
ATCCTCAAGG CTGAGATTGA AGAGAAGGGC TCAGATGGCG CTGCATCCAG TTATCAGCTC
AAGCAGCTTG AGGAGCAGAA TGCCCGCCTG AAGGATGCCC TGGTGAGGAT GCGGGATCTT
TCTTCCTCAG AGAAGCAGGA GCATGTGAAG CTCCAGAAGC TCATGGAAAA GAAGAACCAA
GAGCTGGAAG TTGTGAGGCA ACAGCGGGAG CGTCTGCAGG AGGAGCTAAG CCAGGCAGAG
AGCACCATTG ATGAGCTCAA GGAGCAGGTG GATGCTGCTC TGGGTGCTGA GGAGATGGTG
GAGATGCTGA CAGATCGGAA CCTGAATCTG GAAGAGAAAG TGCGCGAGTT GAGGGAGACT
GTGGGAGACT TGGAAGCGAT GAATGAGATG AACGATGAGC TGCAGGAGAA TGCACGTGAG
ACAGAACTGG AGCTGCGGGA GCAGCTGGAC ATGGCAGGCG CGCGGGTTCG TGAGGCCCAG
AAGCGTGTGG AGGCAGCCCA GGAGACGGTT GCAGACTACC AGCAGACCAT CAAGAAGTAC
CGCCAGCTGA CCGCCCATCT ACAGGATGTG AATCGGGAAC TGACAAACCA GCAGGAAGCA
TCTGTGGAGA GGCAACAGCA GCCACCTCCA GAGACCTTTG ACTTCAAAAT CAAGTTTGCT
GAGACTAAGG CCCATGCCAA GGCAATTGAG ATGGAATTGA GGCAGATGGA GGTGGCCCAG
GCCAATCGAC ACATGTCCCT GCTGACAGCC TTCATGCCTG ACAGCTTCCT TCGGCCAGGT
GGGGACCATG ACTGCGTTCT GGTGCTGTTG CTCATGCCTC GTCTCATTTG CAAGGCAGAG
CTGATCCGGA AGCAGGCCCA GGAGAAGTTT GAACTAAGTG AGAACTGTTC AGAGCGGCCT
GGGCTGCGAG GAGCTGCTGG GGAGCAACTC AGCTTTGCTG CTGGACTGGT GTACTCGCTG
AGCCTGCTGC AGGCCACGCT ACACCGCTAT GAGCATGCCC TCTCTCAGTG CAGTGTGGAT
GTGTATAAGA AAGTGGGCAG CCTGTACCCT GAGATGAGTG CCCATGAGCG CTCCTTGGAT
TTCCTCATTG AACTGCTGCA CAAGGATCAG CTGGATGAGA CTGTCAATGT GGAGCCTCTC
ACCAAGGCCA TCAAGTACTA TCAGCATCTG TACAGCATCC ACCTTGCCGA ACAGCCTGAG
GACTGTACTA TGCAGCTGGC TGACCACATT AAGTTCACGC AGAGTGCTCT GGACTGCATG
AGTGTGGAGG TAGGACGGCT GCGTGCCTTC TTGCAGGGTG GGCAGGAGGC TACAGATATT
GCCCTCCTGC TCCGGGATCT GGAAACTTCA TGCAGTGACA TCCGCCAGTT CTGCAAGAAG
ATCCGAAGGC GAATGCCAGG GACAGATGCT CCTGGGATCC CAGCTGCACT GGCCTTTGGA
CCACAGGTAT CTGACACGCT CCTAGACTGC AGGAAACACT TGACGTGGGT CGTGGCTGTG
CTGCAGGAGG TGGCAGCTGC TGCTGCCCAG CTCATTGCCC CACTGGCAGA GAATGAGGGG
CTACTTGTGG CTGCTCTGGA GGAACTGGCT TTCAAAGCAA GCGAGCAGAT CTATGGGACC
CCCTCCAGCA GCCCCTATGA GTGTCTGCGC CAGTCATGCA ACATCCTCAT CAGTACCATG
AACAAGCTGG CCACAGCCAT GCAGGAGGGG GAGTATGATG CAGAGCGGCC CCCCAGCAAG
CCTCCACCGG TTGAACTGCG GGCTGCTGCC CTTCGTGCAG AGATCACAGA TGCTGAAGGC
CTGGGTTTGA AGCTCGAAGA TCGAGAGACA GTTATTAAGG AGTTGAAGAA GTCACTCAAG
ATTAAGGGAG AGGAGCTAAG TGAGGCCAAT GTGCGGCTGA GCCTCCTGGA GAAGAAGTTG
GACAGTGCTG CCAAGGATGC AGATGAGCGC ATCGAGAAAG TCCAGACTCG GCTGGAGGAG
ACCCAGGCAC TGCTGCGAAA GAAGGAGAAA GAGTTTGAGG AGACAATGGA TGCACTCCAG
GCTGACATCG ACCAGCTGGA GGCAGAGAAG GCAGAACTAA AGCAGCGTCT GAACAGCCAG
TCCAAACGCA CGATTGAGGG ACTCCGGGGC CCTCCTCCTT CAGGCATTGC TACTCTGGTC
TCTGGCATTG CTGGTGAAGA ACAGCAGCGA GGAGCCATCC CTGGGCAGGC TCCAGGGTCT
GTGCCAGGCC CAGGGCTGGT GAAGGACTCA CCACTGCTGC TTCAGCAGAT CTCTGCCATG
AGGCTGCACA TCTCCCAGCT CCAGCATGAG AACAGCATCC TCAAGGGAGC CCAGATGAAG
GCATCCTTGG CATCCCTGCC CCCTCTGCAT GTTGCAAAGC TATCCCATGA GGGCCCTGGC
AGTGAGTTAC CAGCTGGAGC GCTGTATCGT AAGACCAGCC AGCTGCTGGA GACATTGAAT
CAATTGAGCA CACACACGCA CGTAGTAGAC ATCACTCGCA CCAGCCCTGC TGCCAAGAGC
CCGTCGGCCC AACTTATGGA GCAAGTGGCT CAGCTTAAGT CCCTGAGTGA CACCGTCGAG
AAGCTCAAGG ATGAGGTCCT CAAGGAGACA GTATCTCAGC GCCCTGGAGC CACAGTACCC
ACTGACTTTG CCACCTTCCC TTCATCAGCC TTCCTCAGGG CCAAGGAGGA GCAGCAGGAT
GACACAGTCT ACATGGGCAA AGTGACCTTC TCATGTGCGG CTGGTTTTGG ACAGCGACAC
CGGCTGGTGC TGACCCAGGA GCAGCTGCAC CAGCTTCACA GTCGCCTCAT CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004073.2
CDS319..4155
Misc Feature(1)253..255(+)
Misc Feature(2)403..603(+)
Misc Feature(3)640..642(+)
Misc Feature(4)949..>1308(+)
Misc Feature(5)1054..1440(+)
Misc Feature(6)1072..1611(+)
Misc Feature(7)1258..1815(+)
Misc Feature(8)1894..2733(+)
Misc Feature(9)3235..3462(+)
Misc Feature(10)3304..3306(+)
Exon (1)1..351
Gene:DCTN1
Gene Synonym:
Exon (2)352..597
Gene:DCTN1
Gene Synonym:
Exon (3)598..676
Gene:DCTN1
Gene Synonym:
Exon (4)677..711
Gene:DCTN1
Gene Synonym:
Exon (5)712..732
Gene:DCTN1
Gene Synonym:
Exon (6)733..750
Gene:DCTN1
Gene Synonym:
Exon (7)751..771
Gene:DCTN1
Gene Synonym:
Exon (8)772..963
Gene:DCTN1
Gene Synonym:
Exon (9)964..1161
Gene:DCTN1
Gene Synonym:
Exon (10)1162..1366
Gene:DCTN1
Gene Synonym:
Exon (11)1367..1445
Gene:DCTN1
Gene Synonym:
Exon (12)1446..1605
Gene:DCTN1
Gene Synonym:
Exon (13)1606..1710
Gene:DCTN1
Gene Synonym:
Exon (14)1711..1902
Gene:DCTN1
Gene Synonym:
Exon (15)1903..2019
Gene:DCTN1
Gene Synonym:
Exon (16)2020..2172
Gene:DCTN1
Gene Synonym:
Exon (17)2173..2333
Gene:DCTN1
Gene Synonym:
Exon (18)2334..2502
Gene:DCTN1
Gene Synonym:
Exon (19)2503..2571
Gene:DCTN1
Gene Synonym:
Exon (20)2572..2634
Gene:DCTN1
Gene Synonym:
Exon (21)2635..2784
Gene:DCTN1
Gene Synonym:
Exon (22)2785..2946
Gene:DCTN1
Gene Synonym:
Exon (23)2947..3078
Gene:DCTN1
Gene Synonym:
Exon (24)3079..3204
Gene:DCTN1
Gene Synonym:
Exon (25)3205..3347
Gene:DCTN1
Gene Synonym:
Exon (26)3348..3514
Gene:DCTN1
Gene Synonym:
Exon (27)3515..3529
Gene:DCTN1
Gene Synonym:
Exon (28)3530..3663
Gene:DCTN1
Gene Synonym:
Exon (29)3664..3847
Gene:DCTN1
Gene Synonym:
Exon (30)3848..3927
Gene:DCTN1
Gene Synonym:
Exon (31)3928..4017
Gene:DCTN1
Gene Synonym:
Exon (32)4018..4500
Gene:DCTN1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004082
Organism Homo sapiens (human)
Definition Homo sapiens dynactin 1 (DCTN1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004082

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGCGGGGAC TGAAGCCTAA GAAGGCACCG
ACAGCCCGAA AGACCACAAC TCGGCGACCC AAGCCCACGC GCCCAGCCAG TACTGGGGTG
GCTGGGGCCA GTAGCTCCCT GGGCCCCTCT GGCTCAGCGT CAGCAGGTGA GCTGAGCAGC
AGTGAGCCCA GCACCCCGGC TCAGACTCCG CTGGCAGCAC CCATCATCCC CACGCCGGTC
CTCACCTCTC CTGGAGCAGT CCCCCCGCTT CCTTCCCCAT CCAAGGAGGA GGAGGGACTA
AGGGCTCAGG TGCGGGACCT GGAGGAGAAA CTAGAGACCC TGAGACTGAA ACGGGCAGAA
GACAAAGCAA AGCTAAAAGA GCTGGAGAAA CACAAAATCC AGCTGGAGCA GGTGCAGGAA
TGGAAGAGCA AAATGCAGGA GCAGCAGGCC GACCTGCAGC GGCGCCTCAA GGAGGCGAGA
AAGGAAGCCA AGGAGGCGCT GGAGGCAAAG GAACGCTATA TGGAGGAGAT GGCTGATACT
GCTGATGCCA TTGAGATGGC CACTTTGGAC AAGGAGATGG CTGAAGAGCG GGCTGAGTCC
CTGCAGCAGG AGGTGGAGGC ACTGAAGGAG CGGGTGGACG AGCTCACTAC TGACTTAGAG
ATCCTCAAGG CTGAGATTGA AGAGAAGGGC TCAGATGGCG CTGCATCCAG TTATCAGCTC
AAGCAGCTTG AGGAGCAGAA TGCCCGCCTG AAGGATGCCC TGGTGAGGAT GCGGGATCTT
TCTTCCTCAG AGAAGCAGGA GCATGTGAAG CTCCAGAAGC TCATGGAAAA GAAGAACCAA
GAGCTGGAAG TTGTGAGGCA ACAGCGGGAG CGTCTGCAGG AGGAGCTAAG CCAGGCAGAG
AGCACCATTG ATGAGCTCAA GGAGCAGGTG GATGCTGCTC TGGGTGCTGA GGAGATGGTG
GAGATGCTGA CAGATCGGAA CCTGAATCTG GAAGAGAAAG TGCGCGAGTT GAGGGAGACT
GTGGGAGACT TGGAAGCGAT GAATGAGATG AACGATGAGC TGCAGGAGAA TGCACGTGAG
ACAGAACTGG AGCTGCGGGA GCAGCTGGAC ATGGCAGGCG CGCGGGTTCG TGAGGCCCAG
AAGCGTGTGG AGGCAGCCCA GGAGACGGTT GCAGACTACC AGCAGACCAT CAAGAAGTAC
CGCCAGCTGA CCGCCCATCT ACAGGATGTG AATCGGGAAC TGACAAACCA GCAGGAAGCA
TCTGTGGAGA GGCAACAGCA GCCACCTCCA GAGACCTTTG ACTTCAAAAT CAAGTTTGCT
GAGACTAAGG CCCATGCCAA GGCAATTGAG ATGGAATTGA GGCAGATGGA GGTGGCCCAG
GCCAATCGAC ACATGTCCCT GCTGACAGCC TTCATGCCTG ACAGCTTCCT TCGGCCAGGT
GGGGACCATG ACTGCGTTCT GGTGCTGTTG CTCATGCCTC GTCTCATTTG CAAGGCAGAG
CTGATCCGGA AGCAGGCCCA GGAGAAGTTT GAACTAAGTG AGAACTGTTC AGAGCGGCCT
GGGCTGCGAG GAGCTGCTGG GGAGCAACTC AGCTTTGCTG CTGGACTGGT GTACTCGCTG
AGCCTGCTGC AGGCCACGCT ACACCGCTAT GAGCATGCCC TCTCTCAGTG CAGTGTGGAT
GTGTATAAGA AAGTGGGCAG CCTGTACCCT GAGATGAGTG CCCATGAGCG CTCCTTGGAT
TTCCTCATTG AACTGCTGCA CAAGGATCAG CTGGATGAGA CTGTCAATGT GGAGCCTCTC
ACCAAGGCCA TCAAGTACTA TCAGCATCTG TACAGCATCC ACCTTGCCGA ACAGCCTGAG
GACTGTACTA TGCAGCTGGC TGACCACATT AAGTTCACGC AGAGTGCTCT GGACTGCATG
AGTGTGGAGG TAGGACGGCT GCGTGCCTTC TTGCAGGGTG GGCAGGAGGC TACAGATATT
GCCCTCCTGC TCCGGGATCT GGAAACTTCA TGCAGTGACA TCCGCCAGTT CTGCAAGAAG
ATCCGAAGGC GAATGCCAGG GACAGATGCT CCTGGGATCC CAGCTGCACT GGCCTTTGGA
CCACAGGTAT CTGACACGCT CCTAGACTGC AGGAAACACT TGACGTGGGT CGTGGCTGTG
CTGCAGGAGG TGGCAGCTGC TGCTGCCCAG CTCATTGCCC CACTGGCAGA GAATGAGGGG
CTACTTGTGG CTGCTCTGGA GGAACTGGCT TTCAAAGCAA GCGAGCAGAT CTATGGGACC
CCCTCCAGCA GCCCCTATGA GTGTCTGCGC CAGTCATGCA ACATCCTCAT CAGTACCATG
AACAAGCTGG CCACAGCCAT GCAGGAGGGG GAGTATGATG CAGAGCGGCC CCCCAGCAAG
CCTCCACCGG TTGAACTGCG GGCTGCTGCC CTTCGTGCAG AGATCACAGA TGCTGAAGGC
CTGGGTTTGA AGCTCGAAGA TCGAGAGACA GTTATTAAGG AGTTGAAGAA GTCACTCAAG
ATTAAGGGAG AGGAGCTAAG TGAGGCCAAT GTGCGGCTGA GCCTCCTGGA GAAGAAGTTG
GACAGTGCTG CCAAGGATGC AGATGAGCGC ATCGAGAAAG TCCAGACTCG GCTGGAGGAG
ACCCAGGCAC TGCTGCGAAA GAAGGAGAAA GAGTTTGAGG AGACAATGGA TGCACTCCAG
GCTGACATCG ACCAGCTGGA GGCAGAGAAG GCAGAACTAA AGCAGCGTCT GAACAGCCAG
TCCAAACGCA CGATTGAGGG ACTCCGGGGC CCTCCTCCTT CAGGCATTGC TACTCTGGTC
TCTGGCATTG CTGGTGAAGA ACAGCAGCGA GGAGCCATCC CTGGGCAGGC TCCAGGGTCT
GTGCCAGGCC CAGGGCTGGT GAAGGACTCA CCACTGCTGC TTCAGCAGAT CTCTGCCATG
AGGCTGCACA TCTCCCAGCT CCAGCATGAG AACAGCATCC TCAAGGGAGC CCAGATGAAG
GCATCCTTGG CATCCCTGCC CCCTCTGCAT GTTGCAAAGC TATCCCATGA GGGCCCTGGC
AGTGAGTTAC CAGCTGGAGC GCTGTATCGT AAGACCAGCC AGCTGCTGGA GACATTGAAT
CAATTGAGCA CACACACGCA CGTAGTAGAC ATCACTCGCA CCAGCCCTGC TGCCAAGAGC
CCGTCGGCCC AACTTATGGA GCAAGTGGCT CAGCTTAAGT CCCTGAGTGA CACCGTCGAG
AAGCTCAAGG ATGAGGTCCT CAAGGAGACA GTATCTCAGC GCCCTGGAGC CACAGTACCC
ACTGACTTTG CCACCTTCCC TTCATCAGCC TTCCTCAGGG CCAAGGAGGA GCAGCAGGAT
GACACAGTCT ACATGGGCAA AGTGACCTTC TCATGTGCGG CTGGTTTTGG ACAGCGACAC
CGGCTGGTGC TGACCCAGGA GCAGCTGCAC CAGCTTCACA GTCGCCTCAT CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16728
Clone ID Related Accession (Same CDS sequence) NM_023019
Accession Version NM_023019.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 14-MAY-2015
Organism Homo sapiens (human)
Product dynactin subunit 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA111497.1, BC071583.1, BC013978.2, X98801.1 and CA448857.1. On Jul 1, 2010 this sequence version replaced gi:205277390. Transcript Variant: This variant (2) contains an additional in-frame exon in the 3' end, compared to variant 4, resulting in a protein (isoform 2) with a longer C-terminus, compared to isoform 4. Isoform 2 is also called p135. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 9799602 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGATGAGAC AGGCACCGAC AGCCCGAAAG ACCACAACTC GGCGACCCAA GCCCACGCGC 
CCAGCCAGTA CTGGGGTGGC TGGGGCCAGT AGCTCCCTGG GCCCCTCTGG CTCAGCGTCA
GCAGGTGAGC TGAGCAGCAG TGAGCCCAGC ACCCCGGCTC AGACTCCGCT GGCAGCACCC
ATCATCCCCA CGCCGGTCCT CACCTCTCCT GGAGCAGTCC CCCCGCTTCC TTCCCCATCC
AAGGAGGAGG AGGGACTAAG GGCTCAGGTG CGGGACCTGG AGGAGAAACT AGAGACCCTG
AGACTGAAAC GGGCAGAAGA CAAAGCAAAG CTAAAAGAGC TGGAGAAACA CAAAATCCAG
CTGGAGCAGG TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCCGA CCTGCAGCGG
CGCCTCAAGG AGGCGAGAAA GGAAGCCAAG GAGGCGCTGG AGGCAAAGGA ACGCTATATG
GAGGAGATGG CTGATACTGC TGATGCCATT GAGATGGCCA CTTTGGACAA GGAGATGGCT
GAAGAGCGGG CTGAGTCCCT GCAGCAGGAG GTGGAGGCAC TGAAGGAGCG GGTGGACGAG
CTCACTACTG ACTTAGAGAT CCTCAAGGCT GAGATTGAAG AGAAGGGCTC AGATGGCGCT
GCATCCAGTT ATCAGCTCAA GCAGCTTGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG
GTGAGGATGC GGGATCTTTC TTCCTCAGAG AAGCAGGAGC ATGTGAAGCT CCAGAAGCTC
ATGGAAAAGA AGAACCAAGA GCTGGAAGTT GTGAGGCAAC AGCGGGAGCG TCTGCAGGAG
GAGCTAAGCC AGGCAGAGAG CACCATTGAT GAGCTCAAGG AGCAGGTGGA TGCTGCTCTG
GGTGCTGAGG AGATGGTGGA GATGCTGACA GATCGGAACC TGAATCTGGA AGAGAAAGTG
CGCGAGTTGA GGGAGACTGT GGGAGACTTG GAAGCGATGA ATGAGATGAA CGATGAGCTG
CAGGAGAATG CACGTGAGAC AGAACTGGAG CTGCGGGAGC AGCTGGACAT GGCAGGCGCG
CGGGTTCGTG AGGCCCAGAA GCGTGTGGAG GCAGCCCAGG AGACGGTTGC AGACTACCAG
CAGACCATCA AGAAGTACCG CCAGCTGACC GCCCATCTAC AGGATGTGAA TCGGGAACTG
ACAAACCAGC AGGAAGCATC TGTGGAGAGG CAACAGCAGC CACCTCCAGA GACCTTTGAC
TTCAAAATCA AGTTTGCTGA GACTAAGGCC CATGCCAAGG CAATTGAGAT GGAATTGAGG
CAGATGGAGG TGGCCCAGGC CAATCGACAC ATGTCCCTGC TGACAGCCTT CATGCCTGAC
AGCTTCCTTC GGCCAGGTGG GGACCATGAC TGCGTTCTGG TGCTGTTGCT CATGCCTCGT
CTCATTTGCA AGGCAGAGCT GATCCGGAAG CAGGCCCAGG AGAAGTTTGA ACTAAGTGAG
AACTGTTCAG AGCGGCCTGG GCTGCGAGGA GCTGCTGGGG AGCAACTCAG CTTTGCTGCT
GGACTGGTGT ACTCGCTGAG CCTGCTGCAG GCCACGCTAC ACCGCTATGA GCATGCCCTC
TCTCAGTGCA GTGTGGATGT GTATAAGAAA GTGGGCAGCC TGTACCCTGA GATGAGTGCC
CATGAGCGCT CCTTGGATTT CCTCATTGAA CTGCTGCACA AGGATCAGCT GGATGAGACT
GTCAATGTGG AGCCTCTCAC CAAGGCCATC AAGTACTATC AGCATCTGTA CAGCATCCAC
CTTGCCGAAC AGCCTGAGGA CTGTACTATG CAGCTGGCTG ACCACATTAA GTTCACGCAG
AGTGCTCTGG ACTGCATGAG TGTGGAGGTA GGACGGCTGC GTGCCTTCTT GCAGGGTGGG
CAGGAGGCTA CAGATATTGC CCTCCTGCTC CGGGATCTGG AAACTTCATG CAGTGACATC
CGCCAGTTCT GCAAGAAGAT CCGAAGGCGA ATGCCAGGGA CAGATGCTCC TGGGATCCCA
GCTGCACTGG CCTTTGGACC ACAGGTATCT GACACGCTCC TAGACTGCAG GAAACACTTG
ACGTGGGTCG TGGCTGTGCT GCAGGAGGTG GCAGCTGCTG CTGCCCAGCT CATTGCCCCA
CTGGCAGAGA ATGAGGGGCT ACTTGTGGCT GCTCTGGAGG AACTGGCTTT CAAAGCAAGC
GAGCAGATCT ATGGGACCCC CTCCAGCAGC CCCTATGAGT GTCTGCGCCA GTCATGCAAC
ATCCTCATCA GTACCATGAA CAAGCTGGCC ACAGCCATGC AGGAGGGGGA GTATGATGCA
GAGCGGCCCC CCAGCAAGCC TCCACCGGTT GAACTGCGGG CTGCTGCCCT TCGTGCAGAG
ATCACAGATG CTGAAGGCCT GGGTTTGAAG CTCGAAGATC GAGAGACAGT TATTAAGGAG
TTGAAGAAGT CACTCAAGAT TAAGGGAGAG GAGCTAAGTG AGGCCAATGT GCGGCTGAGC
CTCCTGGAGA AGAAGTTGGA CAGTGCTGCC AAGGATGCAG ATGAGCGCAT CGAGAAAGTC
CAGACTCGGC TGGAGGAGAC CCAGGCACTG CTGCGAAAGA AGGAGAAAGA GTTTGAGGAG
ACAATGGATG CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAACTAAAG
CAGCGTCTGA ACAGCCAGTC CAAACGCACG ATTGAGGGAC TCCGGGGCCC TCCTCCTTCA
GGCATTGCTA CTCTGGTCTC TGGCATTGCT GGTGAAGAAC AGCAGCGAGG AGCCATCCCT
GGGCAGGCTC CAGGGTCTGT GCCAGGCCCA GGGCTGGTGA AGGACTCACC ACTGCTGCTT
CAGCAGATCT CTGCCATGAG GCTGCACATC TCCCAGCTCC AGCATGAGAA CAGCATCCTC
AAGGGAGCCC AGATGAAGGC ATCCTTGGCA TCCCTGCCCC CTCTGCATGT TGCAAAGCTA
TCCCATGAGG GCCCTGGCAG TGAGTTACCA GCTGGAGCGC TGTATCGTAA GACCAGCCAG
CTGCTGGAGA CATTGAATCA ATTGAGCACA CACACGCACG TAGTAGACAT CACTCGCACC
AGCCCTGCTG CCAAGAGCCC GTCGGCCCAA CTTATGGAGC AAGTGGCTCA GCTTAAGTCC
CTGAGTGACA CCGTCGAGAA GCTCAAGGAT GAGGTCCTCA AGGAGACAGT ATCTCAGCGC
CCTGGAGCCA CAGTACCCAC TGACTTTGCC ACCTTCCCTT CATCAGCCTT CCTCAGGGCC
AAGGAGGAGC AGCAGGATGA CACAGTCTAC ATGGGCAAAG TGACCTTCTC ATGTGCGGCT
GGTTTTGGAC AGCGACACCG GCTGGTGCTG ACCCAGGAGC AGCTGCACCA GCTTCACAGT
CGCCTCATCT CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_075408.1
CDS384..3818
Misc Feature(1)240..242(+)
Misc Feature(2)612..>971(+)
Misc Feature(3)717..1103(+)
Misc Feature(4)735..1274(+)
Misc Feature(5)921..1478(+)
Misc Feature(6)1557..2396(+)
Misc Feature(7)2967..2969(+)
Exon (1)1..413
Gene:DCTN1
Gene Synonym:
Exon (2)414..434
Gene:DCTN1
Gene Synonym:
Exon (3)435..626
Gene:DCTN1
Gene Synonym:
Exon (4)627..824
Gene:DCTN1
Gene Synonym:
Exon (5)825..1029
Gene:DCTN1
Gene Synonym:
Exon (6)1030..1108
Gene:DCTN1
Gene Synonym:
Exon (7)1109..1268
Gene:DCTN1
Gene Synonym:
Exon (8)1269..1373
Gene:DCTN1
Gene Synonym:
Exon (9)1374..1565
Gene:DCTN1
Gene Synonym:
Exon (10)1566..1682
Gene:DCTN1
Gene Synonym:
Exon (11)1683..1835
Gene:DCTN1
Gene Synonym:
Exon (12)1836..1996
Gene:DCTN1
Gene Synonym:
Exon (13)1997..2165
Gene:DCTN1
Gene Synonym:
Exon (14)2166..2234
Gene:DCTN1
Gene Synonym:
Exon (15)2235..2297
Gene:DCTN1
Gene Synonym:
Exon (16)2298..2447
Gene:DCTN1
Gene Synonym:
Exon (17)2448..2609
Gene:DCTN1
Gene Synonym:
Exon (18)2610..2741
Gene:DCTN1
Gene Synonym:
Exon (19)2742..2867
Gene:DCTN1
Gene Synonym:
Exon (20)2868..3010
Gene:DCTN1
Gene Synonym:
Exon (21)3011..3177
Gene:DCTN1
Gene Synonym:
Exon (22)3178..3192
Gene:DCTN1
Gene Synonym:
Exon (23)3193..3326
Gene:DCTN1
Gene Synonym:
Exon (24)3327..3510
Gene:DCTN1
Gene Synonym:
Exon (25)3511..3590
Gene:DCTN1
Gene Synonym:
Exon (26)3591..3680
Gene:DCTN1
Gene Synonym:
Exon (27)3681..4163
Gene:DCTN1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_023019
Organism Homo sapiens (human)
Definition Homo sapiens dynactin 1 (DCTN1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_023019

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGATGAGAC AGGCACCGAC AGCCCGAAAG ACCACAACTC GGCGACCCAA GCCCACGCGC 
CCAGCCAGTA CTGGGGTGGC TGGGGCCAGT AGCTCCCTGG GCCCCTCTGG CTCAGCGTCA
GCAGGTGAGC TGAGCAGCAG TGAGCCCAGC ACCCCGGCTC AGACTCCGCT GGCAGCACCC
ATCATCCCCA CGCCGGTCCT CACCTCTCCT GGAGCAGTCC CCCCGCTTCC TTCCCCATCC
AAGGAGGAGG AGGGACTAAG GGCTCAGGTG CGGGACCTGG AGGAGAAACT AGAGACCCTG
AGACTGAAAC GGGCAGAAGA CAAAGCAAAG CTAAAAGAGC TGGAGAAACA CAAAATCCAG
CTGGAGCAGG TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCCGA CCTGCAGCGG
CGCCTCAAGG AGGCGAGAAA GGAAGCCAAG GAGGCGCTGG AGGCAAAGGA ACGCTATATG
GAGGAGATGG CTGATACTGC TGATGCCATT GAGATGGCCA CTTTGGACAA GGAGATGGCT
GAAGAGCGGG CTGAGTCCCT GCAGCAGGAG GTGGAGGCAC TGAAGGAGCG GGTGGACGAG
CTCACTACTG ACTTAGAGAT CCTCAAGGCT GAGATTGAAG AGAAGGGCTC AGATGGCGCT
GCATCCAGTT ATCAGCTCAA GCAGCTTGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG
GTGAGGATGC GGGATCTTTC TTCCTCAGAG AAGCAGGAGC ATGTGAAGCT CCAGAAGCTC
ATGGAAAAGA AGAACCAAGA GCTGGAAGTT GTGAGGCAAC AGCGGGAGCG TCTGCAGGAG
GAGCTAAGCC AGGCAGAGAG CACCATTGAT GAGCTCAAGG AGCAGGTGGA TGCTGCTCTG
GGTGCTGAGG AGATGGTGGA GATGCTGACA GATCGGAACC TGAATCTGGA AGAGAAAGTG
CGCGAGTTGA GGGAGACTGT GGGAGACTTG GAAGCGATGA ATGAGATGAA CGATGAGCTG
CAGGAGAATG CACGTGAGAC AGAACTGGAG CTGCGGGAGC AGCTGGACAT GGCAGGCGCG
CGGGTTCGTG AGGCCCAGAA GCGTGTGGAG GCAGCCCAGG AGACGGTTGC AGACTACCAG
CAGACCATCA AGAAGTACCG CCAGCTGACC GCCCATCTAC AGGATGTGAA TCGGGAACTG
ACAAACCAGC AGGAAGCATC TGTGGAGAGG CAACAGCAGC CACCTCCAGA GACCTTTGAC
TTCAAAATCA AGTTTGCTGA GACTAAGGCC CATGCCAAGG CAATTGAGAT GGAATTGAGG
CAGATGGAGG TGGCCCAGGC CAATCGACAC ATGTCCCTGC TGACAGCCTT CATGCCTGAC
AGCTTCCTTC GGCCAGGTGG GGACCATGAC TGCGTTCTGG TGCTGTTGCT CATGCCTCGT
CTCATTTGCA AGGCAGAGCT GATCCGGAAG CAGGCCCAGG AGAAGTTTGA ACTAAGTGAG
AACTGTTCAG AGCGGCCTGG GCTGCGAGGA GCTGCTGGGG AGCAACTCAG CTTTGCTGCT
GGACTGGTGT ACTCGCTGAG CCTGCTGCAG GCCACGCTAC ACCGCTATGA GCATGCCCTC
TCTCAGTGCA GTGTGGATGT GTATAAGAAA GTGGGCAGCC TGTACCCTGA GATGAGTGCC
CATGAGCGCT CCTTGGATTT CCTCATTGAA CTGCTGCACA AGGATCAGCT GGATGAGACT
GTCAATGTGG AGCCTCTCAC CAAGGCCATC AAGTACTATC AGCATCTGTA CAGCATCCAC
CTTGCCGAAC AGCCTGAGGA CTGTACTATG CAGCTGGCTG ACCACATTAA GTTCACGCAG
AGTGCTCTGG ACTGCATGAG TGTGGAGGTA GGACGGCTGC GTGCCTTCTT GCAGGGTGGG
CAGGAGGCTA CAGATATTGC CCTCCTGCTC CGGGATCTGG AAACTTCATG CAGTGACATC
CGCCAGTTCT GCAAGAAGAT CCGAAGGCGA ATGCCAGGGA CAGATGCTCC TGGGATCCCA
GCTGCACTGG CCTTTGGACC ACAGGTATCT GACACGCTCC TAGACTGCAG GAAACACTTG
ACGTGGGTCG TGGCTGTGCT GCAGGAGGTG GCAGCTGCTG CTGCCCAGCT CATTGCCCCA
CTGGCAGAGA ATGAGGGGCT ACTTGTGGCT GCTCTGGAGG AACTGGCTTT CAAAGCAAGC
GAGCAGATCT ATGGGACCCC CTCCAGCAGC CCCTATGAGT GTCTGCGCCA GTCATGCAAC
ATCCTCATCA GTACCATGAA CAAGCTGGCC ACAGCCATGC AGGAGGGGGA GTATGATGCA
GAGCGGCCCC CCAGCAAGCC TCCACCGGTT GAACTGCGGG CTGCTGCCCT TCGTGCAGAG
ATCACAGATG CTGAAGGCCT GGGTTTGAAG CTCGAAGATC GAGAGACAGT TATTAAGGAG
TTGAAGAAGT CACTCAAGAT TAAGGGAGAG GAGCTAAGTG AGGCCAATGT GCGGCTGAGC
CTCCTGGAGA AGAAGTTGGA CAGTGCTGCC AAGGATGCAG ATGAGCGCAT CGAGAAAGTC
CAGACTCGGC TGGAGGAGAC CCAGGCACTG CTGCGAAAGA AGGAGAAAGA GTTTGAGGAG
ACAATGGATG CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAACTAAAG
CAGCGTCTGA ACAGCCAGTC CAAACGCACG ATTGAGGGAC TCCGGGGCCC TCCTCCTTCA
GGCATTGCTA CTCTGGTCTC TGGCATTGCT GGTGAAGAAC AGCAGCGAGG AGCCATCCCT
GGGCAGGCTC CAGGGTCTGT GCCAGGCCCA GGGCTGGTGA AGGACTCACC ACTGCTGCTT
CAGCAGATCT CTGCCATGAG GCTGCACATC TCCCAGCTCC AGCATGAGAA CAGCATCCTC
AAGGGAGCCC AGATGAAGGC ATCCTTGGCA TCCCTGCCCC CTCTGCATGT TGCAAAGCTA
TCCCATGAGG GCCCTGGCAG TGAGTTACCA GCTGGAGCGC TGTATCGTAA GACCAGCCAG
CTGCTGGAGA CATTGAATCA ATTGAGCACA CACACGCACG TAGTAGACAT CACTCGCACC
AGCCCTGCTG CCAAGAGCCC GTCGGCCCAA CTTATGGAGC AAGTGGCTCA GCTTAAGTCC
CTGAGTGACA CCGTCGAGAA GCTCAAGGAT GAGGTCCTCA AGGAGACAGT ATCTCAGCGC
CCTGGAGCCA CAGTACCCAC TGACTTTGCC ACCTTCCCTT CATCAGCCTT CCTCAGGGCC
AAGGAGGAGC AGCAGGATGA CACAGTCTAC ATGGGCAAAG TGACCTTCTC ATGTGCGGCT
GGTTTTGGAC AGCGACACCG GCTGGTGCTG ACCCAGGAGC AGCTGCACCA GCTTCACAGT
CGCCTCATCT CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

DCTN1 mutation analysis in families with progressive supranuclear palsy-like phenotypes
JAMA Neurol 71 (2), 208-215 (2014)
Caroppo P, Le Ber I, Clot F, Rivaud-Pechoux S, Camuzat A, De Septenville A, Boutoleau-Bretonniere C, Mourlon V, Sauvee M, Lebouvier T, Bonnet AM, Levy R, Vercelletto M and Brice A.


book

Characterization of functional domains of human EB1 family proteins
J. Biol. Chem. 278 (50), 49721-49731 (2003)
Bu W and Su LK.


book

Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome
Mol. Biol. Cell 13 (10), 3627-3645 (2002)
Askham JM, Vaughan KT, Goodson HV and Morrison EE.


book

Genomic organization of the DCTN1-SLC4A5 locus encoding both NBC4 and p150(Glued)
Cytogenet. Cell Genet. 95 (3-4), 163-168 (2001)
Pushkin A, Abuladze N, Newman D, Tatishchev S and Kurtz I.


book

The genomic structure of DCTN1, a candidate gene for limb-girdle muscular dystrophy (LGMD2B)
Biochim. Biophys. Acta 1442 (2-3), 432-436 (1998)
Tokito MK and Holzbaur EL.


book

Human DCTN1: genomic structure and evaluation as a candidate for Alstrom syndrome
Genomics 53 (3), 359-364 (1998)
Collin GB, Nishina PM, Marshall JD and Naggert JK.


book

Characterization of the p22 subunit of dynactin reveals the localization of cytoplasmic dynein and dynactin to the midbody of dividing cells
J. Cell Biol. 142 (4), 1023-1034 (1998)
Karki S, LaMonte B and Holzbaur EL.


book

Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization
Genomics 31 (3), 398-399 (1996)
Holzbaur EL and Tokito MK.


book

Perry Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Wszolek,Z. and Wider,C.


book

Homology of a 150K cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued
Nature 351 (6327), 579-583 (1991)
Holzbaur EL, Hammarback JA, Paschal BM, Kravit NG, Pfister KK and Vallee RB.


 
*
*
*
*