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MMAA cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MMAA
Entrez Gene ID 166785
Full Name methylmalonic aciduria (cobalamin deficiency) cblA type
Synonyms cblA
General protein information
Preferred Names

methylmalonic aciduria (cobalamin deficiency) cblA type

Names

methylmalonic aciduria type A protein, mitochondrial
mutant adenosylcobalamin

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

4

4q31.21

Summary The protein encoded by this gene is involved in the translocation of cobalamin into the mitochondrion, where it is used in the final steps of adenosylcobalamin synthesis. Adenosylcobalamin is a coenzyme required for the activity of methylmalonyl-CoA mutase. Defects in this gene are a cause of methylmalonic aciduria. [provided by RefSeq, Jul 2008].
Disorder MIM:

607481

Disorder Html: Methylmalonic aciduria, vitamin B12-responsive, 251100 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011531684.2 XP_011529986.1 methylmalonic aciduria type A protein, mitochondrial isoform X1
XM_011531685.2 XP_011529987.1 methylmalonic aciduria type A protein, mitochondrial isoform X1
XM_011531684.1 XP_011529986.1 methylmalonic aciduria type A protein, mitochondrial isoform X1
XM_011531686.2 XP_011529988.1 methylmalonic aciduria type A protein, mitochondrial isoform X2
XM_011531685.1 XP_011529987.1 methylmalonic aciduria type A protein, mitochondrial isoform X1
NM_172250.2 NP_758454.1 methylmalonic aciduria type A protein, mitochondrial precursor



Gallus gallus (chicken) MMAA XP_420429.1
Danio rerio (zebrafish) mmaa NP_001098582.1
Pan troglodytes (chimpanzee) MMAA XP_526697.2
Mus musculus (house mouse) Mmaa NP_598584.2
Homo sapiens (human) MMAA NP_758454.1
Canis lupus familiaris (dog) MMAA XP_539752.4
Rattus norvegicus (Norway rat) Mmaa NP_001099644.1
Bos taurus (cattle) MMAA NP_001093812.1
Caenorhabditis elegans (roundworm) mmaa-1 NP_001022302.1
Xenopus tropicalis (tropical clawed frog) mmaa XP_002938979.1


Related articles in PubMed

High resolution melting analysis of the MMAA gene in patients with cblA and in those with undiagnosed methylmalonic aciduria.
Dempsey-Nunez L, Illson ML, Kent J, Huang Q, Brebner A, Watkins D, Gilfix BM, Wittwer CT, Rosenblatt DS
Molecular genetics and metabolism107(3)363-7(2012 Nov)

Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein.
Takahashi-Íñiguez T, García-Arellano H, Trujillo-Roldán MA, Flores ME
Biochemical and biophysical research communications404(1)443-7(2011 Jan)

Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation.
Froese DS, Kochan G, Muniz JR, Wu X, Gileadi C, Ugochukwu E, Krysztofinska E, Gravel RA, Oppermann U, Yue WW
The Journal of biological chemistry285(49)38204-13(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel deletion in the MMAA gene in two ian siblings with vitamin B12-responsive methylmalonic academia was identified. The deletion in exon 4 of the MMAA gene (c.674 delA) is a pathogenic allele via a nucleotide frame shift resulting in a stop codon and termination of protein synthesis 38 nucleotides (12 amino acids) downstream of the deletion.
Title: Identification of a novel deletion in the MMAA gene in two ian siblings with vitamin B12-responsive methylmalonic acidemia.

Ten novel mutational MMAA variants have been identified in patients with methylmalonic aciduria.
Title: High resolution melting analysis of the MMAA gene in patients with cblA and in those with undiagnosed methylmalonic aciduria.

MMAA acts as a chaperone of human MCM protein.
Title: Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein.

Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation.
Title: Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation.

Spondylocostal dysostosis associated with MMAA is presented in a young boy.
Title: Spondylocostal dysostosis associated with methylmalonic aciduria.

The following MMAA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMAA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu26306 NM_172250.2
Latest version!
Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu26306 XM_011531685.2
Latest version!
Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu26306 XM_011531684.2
Latest version!
Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu77338 XM_011531686.2
Latest version!
Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
View Old Accession Versions >>
OHu26306 XM_011531684.1 Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu26306 XM_011531685.1 Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26306
Clone ID Related Accession (Same CDS sequence) XM_011531684.2 , XM_011531685.2 , XM_011531684.1 , XM_011531685.1 , NM_172250.2
Accession Version NM_172250.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-03
Organism Homo sapiens(Human)
Product methylmalonic aciduria type A protein, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK126662.1 and AC093864.3. This sequence is a reference standard in the RefSeqGene project. On Apr 2, 2008 this sequence version replaced NM_172250.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK126662.1, SRR1660805.58613.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_758454.1
CDS86..1342
Misc Feature(1)38..40
Misc Feature(2)341..1339
Misc Feature(3)521..>820
Misc Feature(4)533..559
Exon (1)1..20
Exon (2)21..524
Exon (3)525..647
Exon (4)648..818
Exon (5)819..904
Exon (6)905..1054
Exon (7)1055..5943
Translation

Target ORF information:

RefSeq Version NM_172250.2
Organism Homo sapiens(Human)
Definition Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_172250.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26306
Clone ID Related Accession (Same CDS sequence) XM_011531684.2 , XM_011531685.2 , XM_011531684.1 , XM_011531685.1 , NM_172250.2
Accession Version XM_011531684.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product methylmalonic aciduria type A protein, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529986.1
CDS164..1420
Misc Feature(1)419..1417
Misc Feature(2)599..>898
Misc Feature(3)611..637
Translation

Target ORF information:

RefSeq Version XM_011531684.2
Organism Homo sapiens(Human)
Definition Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531684.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26306
Clone ID Related Accession (Same CDS sequence) XM_011531684.2 , XM_011531685.2 , XM_011531684.1 , XM_011531685.1 , NM_172250.2
Accession Version XM_011531685.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product methylmalonic aciduria type A protein, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529987.1
CDS207..1463
Misc Feature(1)462..1460
Misc Feature(2)642..>941
Misc Feature(3)654..680
Translation

Target ORF information:

RefSeq Version XM_011531685.2
Organism Homo sapiens(Human)
Definition Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531685.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26306
Clone ID Related Accession (Same CDS sequence) XM_011531684.2 , XM_011531685.2 , XM_011531684.1 , XM_011531685.1 , NM_172250.2
Accession Version XM_011531684.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product methylmalonic aciduria type A protein, mitochondrial isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529986.1
Translation

Target ORF information:

RefSeq Version XM_011531684.1
Organism Homo sapiens(Human)
Definition Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531684.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26306
Clone ID Related Accession (Same CDS sequence) XM_011531684.2 , XM_011531685.2 , XM_011531684.1 , XM_011531685.1 , NM_172250.2
Accession Version XM_011531685.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product methylmalonic aciduria type A protein, mitochondrial isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529987.1
Translation

Target ORF information:

RefSeq Version XM_011531685.1
Organism Homo sapiens(Human)
Definition Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531685.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77338
Clone ID Related Accession (Same CDS sequence) XM_011531686.2
Accession Version XM_011531686.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 762bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product methylmalonic aciduria type A protein, mitochondrial isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTTACTG AGAGAGGGCA CAAATTATCT GTGCTAGCTG TGGACCCTTC TTCTTGTACT 
AGTGGTGGAT CACTCTTAGG TGATAAAACC CGAATGACTG AGTTATCAAG AGATATGAAT
GCATACATCA GGCCATCTCC TACTAGAGGA ACTTTAGGAG GCGTGACAAG GACCACAAAT
GAAGCTATTC TGTTGTGTGA AGGAGCGGGA TATGACATAA TTCTTATTGA AACCGTTGGT
GTGGGTCAGT CGGAGTTTGC TGTTGCTGAC ATGGTTGACA TGTTTGTTTT ACTACTGCCA
CCAGCAGGAG GAGATGAGCT GCAGGGTATC AAAAGGGGTA TAATCGAGAT GGCAGATCTG
GTAGCTGTAA CTAAATCTGA TGGAGACTTG ATTGTGCCAG CTCGAAGGAT ACAAGCGGAA
TATGTGAGTG CACTGAAATT ACTCCGCAAA CGTTCACAAG TCTGGAAACC AAAGGTAATT
CGTATTTCTG CCCGAAGTGG AGAGGGGATC TCTGAAATGT GGGATAAAAT GAAAGATTTC
CAGGACCTAA TGCTTGCCAG TGGGGAGCTG ACTGCCAAAC GACGGAAGCA ACAGAAAGTT
TGGATGTGGA ATCTCATTCA GGAAAGTGTG TTAGAGCATT TCAGGACCCA CCCCACAGTC
CGGGAACAGA TTCCACTTCT GGAACAAAAG GTTCTCATTG GGGCCCTGTC CCCAGGACTA
GCAGCAGACT TCTTGTTAAA AGCTTTTAAA AGCAGAGACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529988.1
CDS849..1610
Misc Feature(1)<849..1607
Translation

Target ORF information:

RefSeq Version XM_011531686.2
Organism Homo sapiens(Human)
Definition Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531686.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTTACTG AGAGAGGGCA CAAATTATCT GTGCTAGCTG TGGACCCTTC TTCTTGTACT 
AGTGGTGGAT CACTCTTAGG TGATAAAACC CGAATGACTG AGTTATCAAG AGATATGAAT
GCATACATCA GGCCATCTCC TACTAGAGGA ACTTTAGGAG GCGTGACAAG GACCACAAAT
GAAGCTATTC TGTTGTGTGA AGGAGCGGGA TATGACATAA TTCTTATTGA AACCGTTGGT
GTGGGTCAGT CGGAGTTTGC TGTTGCTGAC ATGGTTGACA TGTTTGTTTT ACTACTGCCA
CCAGCAGGAG GAGATGAGCT GCAGGGTATC AAAAGGGGTA TAATCGAGAT GGCAGATCTG
GTAGCTGTAA CTAAATCTGA TGGAGACTTG ATTGTGCCAG CTCGAAGGAT ACAAGCGGAA
TATGTGAGTG CACTGAAATT ACTCCGCAAA CGTTCACAAG TCTGGAAACC AAAGGTAATT
CGTATTTCTG CCCGAAGTGG AGAGGGGATC TCTGAAATGT GGGATAAAAT GAAAGATTTC
CAGGACCTAA TGCTTGCCAG TGGGGAGCTG ACTGCCAAAC GACGGAAGCA ACAGAAAGTT
TGGATGTGGA ATCTCATTCA GGAAAGTGTG TTAGAGCATT TCAGGACCCA CCCCACAGTC
CGGGAACAGA TTCCACTTCT GGAACAAAAG GTTCTCATTG GGGCCCTGTC CCCAGGACTA
GCAGCAGACT TCTTGTTAAA AGCTTTTAAA AGCAGAGACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

High resolution melting analysis of the MMAA gene in patients with cblA and in those with undiagnosed methylmalonic aciduria.
Molecular genetics and metabolism107(3)363-7(2012 Nov)
Dempsey-Nunez L,Illson ML,Kent J,Huang Q,Brebner A,Watkins D,Gilfix BM,Wittwer CT,Rosenblatt DS


book

Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein.
Biochemical and biophysical research communications404(1)443-7(2011 Jan)
Takahashi-Íñiguez T,García-Arellano H,Trujillo-Roldán MA,Flores ME


book

Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation.
The Journal of biological chemistry285(49)38204-13(2010 Dec)
Froese DS,Kochan G,Muniz JR,Wu X,Gileadi C,Ugochukwu E,Krysztofinska E,Gravel RA,Oppermann U,Yue WW


 
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