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AGL cDNA ORF clone, Homo sapiens (human)

Gene Symbol AGL
Entrez Gene ID 178
Full Name amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Synonyms GDE
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p21

Summary This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

610860

Disorder Html: Glycogen storage disease IIIa, 232400 (3); Glycogen storage disease

mRNA and Protein(s)

mRNA Protein Name
XM_005270557 XP_005270614 glycogen debranching enzyme isoform X1
NM_000028 NP_000019 glycogen debranching enzyme isoform 1
NM_000642 NP_000633 glycogen debranching enzyme isoform 1
NM_000645 NP_000636 glycogen debranching enzyme isoform 2
NM_000646 NP_000637 glycogen debranching enzyme isoform 3
NM_000643 NP_000634 glycogen debranching enzyme isoform 1
NM_000644 NP_000635 glycogen debranching enzyme isoform 1



Homo sapiens (human) AGL NP_000635.2
Pan troglodytes (chimpanzee) AGL XP_001159352.1
Macaca mulatta (Rhesus monkey) AGL XP_001106287.1
Canis lupus familiaris (dog) AGL NP_001041561.1
Bos taurus (cattle) AGL NP_001179400.1
Mus musculus (house mouse) Agl NP_001074795.1
Rattus norvegicus (Norway rat) Agl NP_001102034.1
Danio rerio (zebrafish) agla NP_001166124.1
Drosophila melanogaster (fruit fly) CG9485 NP_726062.2
Caenorhabditis elegans agl-1 NP_496984.1
Xenopus (Silurana) tropicalis (western clawed frog) agl NP_001072609.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu16218
XM_005270557 PREDICTED: Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$274.50-$384.30
$549.00
OHu16218
NM_000028 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$274.50-$384.30
$549.00
OHu16218
NM_000642 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$274.50-$384.30
$549.00
OHu16240 NM_000645 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $923.30
$1319.00
OHu16190 NM_000646 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $923.30
$1319.00
OHu16218
NM_000643 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$274.50-$384.30
$549.00
OHu16218
NM_000644 Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$274.50-$384.30
$549.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16218
Clone ID Related Accession (Same CDS sequence) NM_000028 , NM_000642 , NM_000643 , NM_000644 , XM_005270557
Accession Version XM_005270557.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glycogen debranching enzyme isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005270614.1
CDS94..4692
Misc Feature(1)157..4689(+)
Misc Feature(2)181..444(+)
Misc Feature(3)406..1842(+)
Misc Feature(4)2182..3015(+)
Misc Feature(5)3310..4674(+)
Translation

Target ORF information:

RefSeq Version XM_005270557
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005270557

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16218
Clone ID Related Accession (Same CDS sequence) NM_000028 , NM_000642 , NM_000643 , NM_000644 , XM_005270557
Accession Version NM_000028.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glycogen debranching enzyme isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84010.1 and AC096949.2. On Oct 27, 2006 this sequence version replaced gi:4557274. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (1). Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000019.2
CDS479..5077
Misc Feature(1)449..451(+)
Misc Feature(2)542..5074(+)
Misc Feature(3)566..829(+)
Misc Feature(4)791..2227(+)
Misc Feature(5)2228..2230(+)
Misc Feature(6)2567..3400(+)
Misc Feature(7)3695..5059(+)
Exon (1)1..410
Gene:AGL
Gene Synonym:
Exon (2)411..560
Gene:AGL
Gene Synonym:
Exon (3)561..771
Gene:AGL
Gene Synonym:
Exon (4)772..938
Gene:AGL
Gene Synonym:
Exon (5)939..1142
Gene:AGL
Gene Synonym:
Exon (6)1143..1324
Gene:AGL
Gene Synonym:
Exon (7)1325..1436
Gene:AGL
Gene Synonym:
Exon (8)1437..1560
Gene:AGL
Gene Synonym:
Exon (9)1561..1663
Gene:AGL
Gene Synonym:
Exon (10)1664..1761
Gene:AGL
Gene Synonym:
Exon (11)1762..1901
Gene:AGL
Gene Synonym:
Exon (12)1902..2089
Gene:AGL
Gene Synonym:
Exon (13)2090..2213
Gene:AGL
Gene Synonym:
Exon (14)2214..2377
Gene:AGL
Gene Synonym:
Exon (15)2378..2479
Gene:AGL
Gene Synonym:
Exon (16)2480..2635
Gene:AGL
Gene Synonym:
Exon (17)2636..2786
Gene:AGL
Gene Synonym:
Exon (18)2787..2911
Gene:AGL
Gene Synonym:
Exon (19)2912..3024
Gene:AGL
Gene Synonym:
Exon (20)3025..3159
Gene:AGL
Gene Synonym:
Exon (21)3160..3290
Gene:AGL
Gene Synonym:
Exon (22)3291..3427
Gene:AGL
Gene Synonym:
Exon (23)3428..3561
Gene:AGL
Gene Synonym:
Exon (24)3562..3737
Gene:AGL
Gene Synonym:
Exon (25)3738..3840
Gene:AGL
Gene Synonym:
Exon (26)3841..4066
Gene:AGL
Gene Synonym:
Exon (27)4067..4178
Gene:AGL
Gene Synonym:
Exon (28)4179..4314
Gene:AGL
Gene Synonym:
Exon (29)4315..4427
Gene:AGL
Gene Synonym:
Exon (30)4428..4639
Gene:AGL
Gene Synonym:
Exon (31)4640..4737
Gene:AGL
Gene Synonym:
Exon (32)4738..4825
Gene:AGL
Gene Synonym:
Exon (33)4826..4959
Gene:AGL
Gene Synonym:
Exon (34)4960..7449
Gene:AGL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000028
Organism Homo sapiens (human)
Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000028

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16218
Clone ID Related Accession (Same CDS sequence) NM_000028 , NM_000642 , NM_000643 , NM_000644 , XM_005270557
Accession Version NM_000642.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glycogen debranching enzyme isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84007.1, BC078663.1, AC096949.2, AK130308.1 and C00487.1. This sequence is a reference standard in the RefSeqGene project. On Oct 27, 2006 this sequence version replaced gi:4557276. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84007.1, BC078663.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000633.2
CDS401..4999
Misc Feature(1)371..373(+)
Misc Feature(2)464..4996(+)
Misc Feature(3)488..751(+)
Misc Feature(4)713..2149(+)
Misc Feature(5)2150..2152(+)
Misc Feature(6)2489..3322(+)
Misc Feature(7)3617..4981(+)
Exon (1)1..332
Gene:AGL
Gene Synonym:
Exon (2)333..482
Gene:AGL
Gene Synonym:
Exon (3)483..693
Gene:AGL
Gene Synonym:
Exon (4)694..860
Gene:AGL
Gene Synonym:
Exon (5)861..1064
Gene:AGL
Gene Synonym:
Exon (6)1065..1246
Gene:AGL
Gene Synonym:
Exon (7)1247..1358
Gene:AGL
Gene Synonym:
Exon (8)1359..1482
Gene:AGL
Gene Synonym:
Exon (9)1483..1585
Gene:AGL
Gene Synonym:
Exon (10)1586..1683
Gene:AGL
Gene Synonym:
Exon (11)1684..1823
Gene:AGL
Gene Synonym:
Exon (12)1824..2011
Gene:AGL
Gene Synonym:
Exon (13)2012..2135
Gene:AGL
Gene Synonym:
Exon (14)2136..2299
Gene:AGL
Gene Synonym:
Exon (15)2300..2401
Gene:AGL
Gene Synonym:
Exon (16)2402..2557
Gene:AGL
Gene Synonym:
Exon (17)2558..2708
Gene:AGL
Gene Synonym:
Exon (18)2709..2833
Gene:AGL
Gene Synonym:
Exon (19)2834..2946
Gene:AGL
Gene Synonym:
Exon (20)2947..3081
Gene:AGL
Gene Synonym:
Exon (21)3082..3212
Gene:AGL
Gene Synonym:
Exon (22)3213..3349
Gene:AGL
Gene Synonym:
Exon (23)3350..3483
Gene:AGL
Gene Synonym:
Exon (24)3484..3659
Gene:AGL
Gene Synonym:
Exon (25)3660..3762
Gene:AGL
Gene Synonym:
Exon (26)3763..3988
Gene:AGL
Gene Synonym:
Exon (27)3989..4100
Gene:AGL
Gene Synonym:
Exon (28)4101..4236
Gene:AGL
Gene Synonym:
Exon (29)4237..4349
Gene:AGL
Gene Synonym:
Exon (30)4350..4561
Gene:AGL
Gene Synonym:
Exon (31)4562..4659
Gene:AGL
Gene Synonym:
Exon (32)4660..4747
Gene:AGL
Gene Synonym:
Exon (33)4748..4881
Gene:AGL
Gene Synonym:
Exon (34)4882..7371
Gene:AGL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000642
Organism Homo sapiens (human)
Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000642

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16240
Clone ID Related Accession (Same CDS sequence) NM_000645
Accession Version NM_000645.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glycogen debranching enzyme isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC096949.2 and M85168.1. On Oct 27, 2006 this sequence version replaced gi:4557282. Transcript Variant: This variant (5) differs in the 5' UTR and coding region, compared to variant 4. The resulting protein (isoform 2) is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M85168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGAGTTTAT TAACATGTGC TTTTTATTTA GGGTATGAGC TACAGTTCCG ATTAGGCCCA 
ACTTTACAGG GAAAAGCAGT TACCGTGTAT ACAAATTACC CATTTCCTGG AGAAACATTT
AATAGAGAAA AATTCCGTTC TCTGGATTGG GAAAATCCAA CAGAAAGAGA AGATGATTCT
GATAAATACT GTAAACTTAA TCTGCAACAA TCTGGTTCAT TTCAGTATTA TTTCCTTCAA
GGAAATGAGA AAAGTGGTGG AGGTTACATA GTTGTGGACC CCATTTTACG TGTTGGTGCT
GATAATCATG TGCTACCCTT GGACTGTGTT ACTCTTCAGA CATTTTTAGC TAAGTGTTTG
GGACCTTTTG ATGAATGGGA AAGCAGACTT AGGGTTGCAA AAGAATCAGG CTACAACATG
ATTCATTTTA CCCCATTGCA GACTCTTGGA CTATCTAGGT CATGCTACTC CCTTGCCAAT
CAGTTAGAAT TAAATCCTGA CTTTTCAAGA CCTAATAGAA AGTATACCTG GAATGATGTT
GGACAGCTAG TGGAAAAATT AAAAAAGGAA TGGAATGTTA TTTGTATTAC TGATGTTGTC
TACAATCATA CTGCTGCTAA TAGTAAATGG ATCCAGGAAC ATCCAGAATG TGCCTATAAT
CTTGTGAATT CTCCACACTT AAAACCTGCC TGGGTCTTAG ACAGAGCACT TTGGCGTTTC
TCCTGTGATG TTGCAGAAGG GAAATACAAA GAAAAGGGAA TACCTGCTTT GATTGAAAAT
GATCACCATA TGAATTCCAT CCGAAAAATA ATTTGGGAGG ATATTTTTCC AAAGCTTAAA
CTCTGGGAAT TTTTCCAAGT AGATGTCAAC AAAGCGGTTG AGCAATTTAG AAGACTTCTT
ACACAAGAAA ATAGGCGAGT AACCAAGTCT GATCCAAACC AACACCTTAC GATTATTCAA
GATCCTGAAT ACAGACGGTT TGGCTGTACT GTAGATATGA ACATTGCACT AACGACTTTC
ATACCACATG ACAAGGGGCC AGCAGCAATT GAAGAATGCT GTAATTGGTT TCATAAAAGA
ATGGAGGAAT TAAATTCAGA GAAGCATCGA CTCATTAACT ATCATCAGGA ACAGGCAGTT
AATTGCCTTT TGGGAAATGT GTTTTATGAA CGACTGGCTG GCCATGGTCC AAAACTAGGA
CCTGTCACTA GAAAGCATCC TTTAGTTACC AGGTATTTTA CTTTCCCATT TGAAGAGATA
GACTTCTCCA TGGAAGAATC TATGATTCAT CTGCCAAATA AAGCTTGTTT TCTGATGGCA
CACAATGGAT GGGTAATGGG AGATGATCCT CTTCGAAACT TTGCTGAACC GGGTTCAGAA
GTTTACCTAA GGAGAGAACT TATTTGCTGG GGAGACAGTG TTAAATTACG CTATGGGAAT
AAACCAGAGG ACTGTCCTTA TCTCTGGGCA CACATGAAAA AATACACTGA AATAACTGCA
ACTTATTTCC AGGGAGTACG TCTTGATAAC TGCCACTCAA CACCTCTTCA CGTAGCTGAG
TACATGTTGG ATGCTGCTAG GAATTTGCAA CCCAATTTAT ATGTAGTAGC TGAACTGTTC
ACAGGAAGTG AAGATCTGGA CAATGTCTTT GTTACTAGAC TGGGCATTAG TTCCTTAATA
AGAGAGGCAA TGAGTGCATA TAATAGTCAT GAAGAGGGCA GATTAGTTTA CCGATATGGA
GGAGAACCTG TTGGATCCTT TGTTCAGCCC TGTTTGAGGC CTTTAATGCC AGCTATTGCA
CATGCCCTGT TTATGGATAT TACGCATGAT AATGAGTGTC CTATTGTGCA TAGATCAGCG
TATGATGCTC TTCCAAGTAC TACAATTGTT TCTATGGCAT GTTGTGCTAG TGGAAGTACA
AGAGGCTATG ATGAATTAGT GCCTCATCAG ATTTCAGTGG TTTCTGAAGA ACGGTTTTAC
ACTAAGTGGA ATCCTGAAGC ATTGCCTTCA AACACAGGTG AAGTTAATTT CCAAAGCGGC
ATTATTGCAG CCAGGTGTGC TATCAGTAAA CTTCATCAGG AGCTTGGAGC CAAGGGTTTT
ATTCAGGTGT ATGTGGATCA AGTTGATGAA GACATAGTGG CAGTAACAAG ACACTCACCT
AGCATCCATC AGTCTGTTGT GGCTGTATCT AGAACTGCTT TCAGGAATCC CAAGACTTCA
TTTTACAGCA AGGAAGTGCC TCAAATGTGC ATCCCTGGCA AAATTGAAGA AGTAGTTCTT
GAAGCTAGAA CTATTGAGAG AAACACGAAA CCTTATAGGA AGGATGAGAA TTCAATCAAT
GGAACACCAG ATATCACAGT AGAAATTAGA GAACATATTC AGCTTAATGA AAGTAAAATT
GTTAAACAAG CTGGAGTTGC CACAAAAGGG CCCAATGAAT ATATTCAAGA AATAGAATTT
GAAAACTTGT CTCCAGGAAG TGTTATTATA TTCAGAGTTA GTCTTGATCC ACATGCACAA
GTCGCTGTTG GAATTCTTCG AAATCATCTG ACACAATTCA GTCCTCACTT TAAATCTGGC
AGCCTAGCTG TTGACAATGC AGATCCTATA TTAAAAATTC CTTTTGCTTC TCTTGCCTCC
AGATTAACTT TGGCTGAGCT AAATCAGATC CTTTACCGAT GTGAATCAGA AGAAAAGGAA
GATGGTGGAG GGTGCTATGA CATACCAAAC TGGTCAGCCC TTAAATATGC AGGTCTTCAA
GGTTTAATGT CTGTATTGGC AGAAATAAGA CCAAAGAATG ACTTGGGGCA TCCTTTTTGT
AATAATTTGA GATCTGGAGA TTGGATGATT GACTATGTCA GTAACCGGCT TATTTCACGA
TCAGGAACTA TTGCTGAAGT TGGTAAATGG TTGCAGGCTA TGTTCTTCTA CCTGAAGCAG
ATCCCACGTT ACCTTATCCC ATGTTACTTT GATGCTATAT TAATTGGTGC ATATACCACT
CTTCTGGATA CAGCATGGAA GCAGATGTCA AGCTTTGTTC AGAATGGTTC AACCTTTGTG
AAACACCTTT CATTGGGTTC AGTTCAACTG TGTGGAGTAG GAAAATTCCC TTCCCTGCCA
ATTCTTTCAC CTGCCCTAAT GGATGTACCT TATAGGTTAA ATGAGATCAC AAAAGAAAAG
GAGCAATGTT GTGTTTCTCT AGCTGCAGGC TTACCTCATT TTTCTTCTGG TATTTTCCGC
TGCTGGGGAA GGGATACTTT TATTGCACTT AGAGGTATAC TGCTGATTAC TGGACGCTAT
GTAGAAGCCA GGAATATTAT TTTAGCATTT GCGGGTACCC TGAGGCATGG TCTCATTCCT
AATCTACTGG GTGAAGGAAT TTATGCCAGA TACAATTGTC GGGATGCTGT GTGGTGGTGG
CTGCAGTGTA TCCAGGATTA CTGTAAAATG GTTCCAAATG GTCTAGACAT TCTCAAGTGC
CCAGTTTCCA GAATGTATCC TACAGATGAT TCTGCTCCTT TGCCTGCTGG CACACTGGAT
CAGCCATTGT TTGAAGTCAT ACAGGAAGCA ATGCAAAAAC ACATGCAGGG CATACAGTTC
CGAGAAAGGA ATGCTGGTCC CCAGATAGAT CGAAACATGA AGGACGAAGG TTTTAATATA
ACTGCAGGAG TTGATGAAGA AACAGGATTT GTTTATGGAG GAAATCGTTT CAATTGTGGC
ACATGGATGG ATAAAATGGG AGAAAGTGAC AGAGCTAGAA ACAGAGGAAT CCCAGCCACA
CCAAGAGATG GGTCTGCTGT GGAAATTGTG GGCCTGAGTA AATCTGCTGT TCGCTGGTTG
CTGGAATTAT CCAAAAAAAA TATTTTCCCT TATCATGAAG TCACAGTAAA AAGACATGGA
AAGGCTATAA AGGTCTCATA TGATGAGTGG AACAGAAAAA TACAAGACAA CTTTGAAAAG
CTATTTCATG TTTCCGAAGA CCCTTCAGAT TTAAATGAAA AGCATCCAAA TCTGGTTCAC
AAACGTGGCA TATACAAAGA TAGTTATGGA GCTTCAAGTC CTTGGTGTGA CTATCAGCTC
AGGCCTAATT TTACCATAGC AATGGTTGTG GCCCCTGAGC TCTTTACTAC AGAAAAAGCA
TGGAAAGCTT TGGAGATTGC AGAAAAAAAA TTGCTTGGTC CCCTTGGCAT GAAAACTTTA
GATCCAGATG ATATGGTTTA CTGTGGAATT TATGACAATG CATTAGACAA TGACAACTAC
AATCTTGCTA AAGGTTTCAA TTATCACCAA GGACCTGAGT GGCTGTGGCC TATTGGGTAT
TTTCTTCGTG CAAAATTATA TTTTTCCAGA TTGATGGGCC CGGAGACTAC TGCAAAGACT
ATAGTTTTGG TTAAAAATGT TCTTTCCCGA CATTATGTTC ATCTTGAGAG ATCCCCTTGG
AAAGGACTTC CAGAACTGAC CAATGAGAAT GCCCAGTACT GTCCTTTCAG CTGTGAAACA
CAAGCCTGGT CAATTGCTAC TATTCTTGAG ACACTTTATG ATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000636.2
CDS263..4810
Misc Feature(1)164..166(+)
Misc Feature(2)293..4807(+)
Misc Feature(3)299..562(+)
Misc Feature(4)524..1960(+)
Misc Feature(5)1961..1963(+)
Misc Feature(6)2300..3133(+)
Misc Feature(7)3428..4792(+)
Exon (1)1..504
Gene:AGL
Gene Synonym:
Exon (2)505..671
Gene:AGL
Gene Synonym:
Exon (3)672..875
Gene:AGL
Gene Synonym:
Exon (4)876..1057
Gene:AGL
Gene Synonym:
Exon (5)1058..1169
Gene:AGL
Gene Synonym:
Exon (6)1170..1293
Gene:AGL
Gene Synonym:
Exon (7)1294..1396
Gene:AGL
Gene Synonym:
Exon (8)1397..1494
Gene:AGL
Gene Synonym:
Exon (9)1495..1634
Gene:AGL
Gene Synonym:
Exon (10)1635..1822
Gene:AGL
Gene Synonym:
Exon (11)1823..1946
Gene:AGL
Gene Synonym:
Exon (12)1947..2110
Gene:AGL
Gene Synonym:
Exon (13)2111..2212
Gene:AGL
Gene Synonym:
Exon (14)2213..2368
Gene:AGL
Gene Synonym:
Exon (15)2369..2519
Gene:AGL
Gene Synonym:
Exon (16)2520..2644
Gene:AGL
Gene Synonym:
Exon (17)2645..2757
Gene:AGL
Gene Synonym:
Exon (18)2758..2892
Gene:AGL
Gene Synonym:
Exon (19)2893..3023
Gene:AGL
Gene Synonym:
Exon (20)3024..3160
Gene:AGL
Gene Synonym:
Exon (21)3161..3294
Gene:AGL
Gene Synonym:
Exon (22)3295..3470
Gene:AGL
Gene Synonym:
Exon (23)3471..3573
Gene:AGL
Gene Synonym:
Exon (24)3574..3799
Gene:AGL
Gene Synonym:
Exon (25)3800..3911
Gene:AGL
Gene Synonym:
Exon (26)3912..4047
Gene:AGL
Gene Synonym:
Exon (27)4048..4160
Gene:AGL
Gene Synonym:
Exon (28)4161..4372
Gene:AGL
Gene Synonym:
Exon (29)4373..4470
Gene:AGL
Gene Synonym:
Exon (30)4471..4558
Gene:AGL
Gene Synonym:
Exon (31)4559..4692
Gene:AGL
Gene Synonym:
Exon (32)4693..7182
Gene:AGL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000645
Organism Homo sapiens (human)
Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000645

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGAGTTTAT TAACATGTGC TTTTTATTTA GGGTATGAGC TACAGTTCCG ATTAGGCCCA 
ACTTTACAGG GAAAAGCAGT TACCGTGTAT ACAAATTACC CATTTCCTGG AGAAACATTT
AATAGAGAAA AATTCCGTTC TCTGGATTGG GAAAATCCAA CAGAAAGAGA AGATGATTCT
GATAAATACT GTAAACTTAA TCTGCAACAA TCTGGTTCAT TTCAGTATTA TTTCCTTCAA
GGAAATGAGA AAAGTGGTGG AGGTTACATA GTTGTGGACC CCATTTTACG TGTTGGTGCT
GATAATCATG TGCTACCCTT GGACTGTGTT ACTCTTCAGA CATTTTTAGC TAAGTGTTTG
GGACCTTTTG ATGAATGGGA AAGCAGACTT AGGGTTGCAA AAGAATCAGG CTACAACATG
ATTCATTTTA CCCCATTGCA GACTCTTGGA CTATCTAGGT CATGCTACTC CCTTGCCAAT
CAGTTAGAAT TAAATCCTGA CTTTTCAAGA CCTAATAGAA AGTATACCTG GAATGATGTT
GGACAGCTAG TGGAAAAATT AAAAAAGGAA TGGAATGTTA TTTGTATTAC TGATGTTGTC
TACAATCATA CTGCTGCTAA TAGTAAATGG ATCCAGGAAC ATCCAGAATG TGCCTATAAT
CTTGTGAATT CTCCACACTT AAAACCTGCC TGGGTCTTAG ACAGAGCACT TTGGCGTTTC
TCCTGTGATG TTGCAGAAGG GAAATACAAA GAAAAGGGAA TACCTGCTTT GATTGAAAAT
GATCACCATA TGAATTCCAT CCGAAAAATA ATTTGGGAGG ATATTTTTCC AAAGCTTAAA
CTCTGGGAAT TTTTCCAAGT AGATGTCAAC AAAGCGGTTG AGCAATTTAG AAGACTTCTT
ACACAAGAAA ATAGGCGAGT AACCAAGTCT GATCCAAACC AACACCTTAC GATTATTCAA
GATCCTGAAT ACAGACGGTT TGGCTGTACT GTAGATATGA ACATTGCACT AACGACTTTC
ATACCACATG ACAAGGGGCC AGCAGCAATT GAAGAATGCT GTAATTGGTT TCATAAAAGA
ATGGAGGAAT TAAATTCAGA GAAGCATCGA CTCATTAACT ATCATCAGGA ACAGGCAGTT
AATTGCCTTT TGGGAAATGT GTTTTATGAA CGACTGGCTG GCCATGGTCC AAAACTAGGA
CCTGTCACTA GAAAGCATCC TTTAGTTACC AGGTATTTTA CTTTCCCATT TGAAGAGATA
GACTTCTCCA TGGAAGAATC TATGATTCAT CTGCCAAATA AAGCTTGTTT TCTGATGGCA
CACAATGGAT GGGTAATGGG AGATGATCCT CTTCGAAACT TTGCTGAACC GGGTTCAGAA
GTTTACCTAA GGAGAGAACT TATTTGCTGG GGAGACAGTG TTAAATTACG CTATGGGAAT
AAACCAGAGG ACTGTCCTTA TCTCTGGGCA CACATGAAAA AATACACTGA AATAACTGCA
ACTTATTTCC AGGGAGTACG TCTTGATAAC TGCCACTCAA CACCTCTTCA CGTAGCTGAG
TACATGTTGG ATGCTGCTAG GAATTTGCAA CCCAATTTAT ATGTAGTAGC TGAACTGTTC
ACAGGAAGTG AAGATCTGGA CAATGTCTTT GTTACTAGAC TGGGCATTAG TTCCTTAATA
AGAGAGGCAA TGAGTGCATA TAATAGTCAT GAAGAGGGCA GATTAGTTTA CCGATATGGA
GGAGAACCTG TTGGATCCTT TGTTCAGCCC TGTTTGAGGC CTTTAATGCC AGCTATTGCA
CATGCCCTGT TTATGGATAT TACGCATGAT AATGAGTGTC CTATTGTGCA TAGATCAGCG
TATGATGCTC TTCCAAGTAC TACAATTGTT TCTATGGCAT GTTGTGCTAG TGGAAGTACA
AGAGGCTATG ATGAATTAGT GCCTCATCAG ATTTCAGTGG TTTCTGAAGA ACGGTTTTAC
ACTAAGTGGA ATCCTGAAGC ATTGCCTTCA AACACAGGTG AAGTTAATTT CCAAAGCGGC
ATTATTGCAG CCAGGTGTGC TATCAGTAAA CTTCATCAGG AGCTTGGAGC CAAGGGTTTT
ATTCAGGTGT ATGTGGATCA AGTTGATGAA GACATAGTGG CAGTAACAAG ACACTCACCT
AGCATCCATC AGTCTGTTGT GGCTGTATCT AGAACTGCTT TCAGGAATCC CAAGACTTCA
TTTTACAGCA AGGAAGTGCC TCAAATGTGC ATCCCTGGCA AAATTGAAGA AGTAGTTCTT
GAAGCTAGAA CTATTGAGAG AAACACGAAA CCTTATAGGA AGGATGAGAA TTCAATCAAT
GGAACACCAG ATATCACAGT AGAAATTAGA GAACATATTC AGCTTAATGA AAGTAAAATT
GTTAAACAAG CTGGAGTTGC CACAAAAGGG CCCAATGAAT ATATTCAAGA AATAGAATTT
GAAAACTTGT CTCCAGGAAG TGTTATTATA TTCAGAGTTA GTCTTGATCC ACATGCACAA
GTCGCTGTTG GAATTCTTCG AAATCATCTG ACACAATTCA GTCCTCACTT TAAATCTGGC
AGCCTAGCTG TTGACAATGC AGATCCTATA TTAAAAATTC CTTTTGCTTC TCTTGCCTCC
AGATTAACTT TGGCTGAGCT AAATCAGATC CTTTACCGAT GTGAATCAGA AGAAAAGGAA
GATGGTGGAG GGTGCTATGA CATACCAAAC TGGTCAGCCC TTAAATATGC AGGTCTTCAA
GGTTTAATGT CTGTATTGGC AGAAATAAGA CCAAAGAATG ACTTGGGGCA TCCTTTTTGT
AATAATTTGA GATCTGGAGA TTGGATGATT GACTATGTCA GTAACCGGCT TATTTCACGA
TCAGGAACTA TTGCTGAAGT TGGTAAATGG TTGCAGGCTA TGTTCTTCTA CCTGAAGCAG
ATCCCACGTT ACCTTATCCC ATGTTACTTT GATGCTATAT TAATTGGTGC ATATACCACT
CTTCTGGATA CAGCATGGAA GCAGATGTCA AGCTTTGTTC AGAATGGTTC AACCTTTGTG
AAACACCTTT CATTGGGTTC AGTTCAACTG TGTGGAGTAG GAAAATTCCC TTCCCTGCCA
ATTCTTTCAC CTGCCCTAAT GGATGTACCT TATAGGTTAA ATGAGATCAC AAAAGAAAAG
GAGCAATGTT GTGTTTCTCT AGCTGCAGGC TTACCTCATT TTTCTTCTGG TATTTTCCGC
TGCTGGGGAA GGGATACTTT TATTGCACTT AGAGGTATAC TGCTGATTAC TGGACGCTAT
GTAGAAGCCA GGAATATTAT TTTAGCATTT GCGGGTACCC TGAGGCATGG TCTCATTCCT
AATCTACTGG GTGAAGGAAT TTATGCCAGA TACAATTGTC GGGATGCTGT GTGGTGGTGG
CTGCAGTGTA TCCAGGATTA CTGTAAAATG GTTCCAAATG GTCTAGACAT TCTCAAGTGC
CCAGTTTCCA GAATGTATCC TACAGATGAT TCTGCTCCTT TGCCTGCTGG CACACTGGAT
CAGCCATTGT TTGAAGTCAT ACAGGAAGCA ATGCAAAAAC ACATGCAGGG CATACAGTTC
CGAGAAAGGA ATGCTGGTCC CCAGATAGAT CGAAACATGA AGGACGAAGG TTTTAATATA
ACTGCAGGAG TTGATGAAGA AACAGGATTT GTTTATGGAG GAAATCGTTT CAATTGTGGC
ACATGGATGG ATAAAATGGG AGAAAGTGAC AGAGCTAGAA ACAGAGGAAT CCCAGCCACA
CCAAGAGATG GGTCTGCTGT GGAAATTGTG GGCCTGAGTA AATCTGCTGT TCGCTGGTTG
CTGGAATTAT CCAAAAAAAA TATTTTCCCT TATCATGAAG TCACAGTAAA AAGACATGGA
AAGGCTATAA AGGTCTCATA TGATGAGTGG AACAGAAAAA TACAAGACAA CTTTGAAAAG
CTATTTCATG TTTCCGAAGA CCCTTCAGAT TTAAATGAAA AGCATCCAAA TCTGGTTCAC
AAACGTGGCA TATACAAAGA TAGTTATGGA GCTTCAAGTC CTTGGTGTGA CTATCAGCTC
AGGCCTAATT TTACCATAGC AATGGTTGTG GCCCCTGAGC TCTTTACTAC AGAAAAAGCA
TGGAAAGCTT TGGAGATTGC AGAAAAAAAA TTGCTTGGTC CCCTTGGCAT GAAAACTTTA
GATCCAGATG ATATGGTTTA CTGTGGAATT TATGACAATG CATTAGACAA TGACAACTAC
AATCTTGCTA AAGGTTTCAA TTATCACCAA GGACCTGAGT GGCTGTGGCC TATTGGGTAT
TTTCTTCGTG CAAAATTATA TTTTTCCAGA TTGATGGGCC CGGAGACTAC TGCAAAGACT
ATAGTTTTGG TTAAAAATGT TCTTTCCCGA CATTATGTTC ATCTTGAGAG ATCCCCTTGG
AAAGGACTTC CAGAACTGAC CAATGAGAAT GCCCAGTACT GTCCTTTCAG CTGTGAAACA
CAAGCCTGGT CAATTGCTAC TATTCTTGAG ACACTTTATG ATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16190
Clone ID Related Accession (Same CDS sequence) NM_000646
Accession Version NM_000646.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glycogen debranching enzyme isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84011.1 and AC096949.2. On or before Aug 31, 2013 this sequence version replaced gi:530361986, gi:4557284. Transcript Variant: This variant (6) differs in the 5' UTR and coding region, compared to variant 4. The resulting protein (isoform 3) is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84011.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGGCCCCAA TTCTGAGCAT TAATTTATTT ATTGGGTATG AGCTACAGTT CCGATTAGGC 
CCAACTTTAC AGGGAAAAGC AGTTACCGTG TATACAAATT ACCCATTTCC TGGAGAAACA
TTTAATAGAG AAAAATTCCG TTCTCTGGAT TGGGAAAATC CAACAGAAAG AGAAGATGAT
TCTGATAAAT ACTGTAAACT TAATCTGCAA CAATCTGGTT CATTTCAGTA TTATTTCCTT
CAAGGAAATG AGAAAAGTGG TGGAGGTTAC ATAGTTGTGG ACCCCATTTT ACGTGTTGGT
GCTGATAATC ATGTGCTACC CTTGGACTGT GTTACTCTTC AGACATTTTT AGCTAAGTGT
TTGGGACCTT TTGATGAATG GGAAAGCAGA CTTAGGGTTG CAAAAGAATC AGGCTACAAC
ATGATTCATT TTACCCCATT GCAGACTCTT GGACTATCTA GGTCATGCTA CTCCCTTGCC
AATCAGTTAG AATTAAATCC TGACTTTTCA AGACCTAATA GAAAGTATAC CTGGAATGAT
GTTGGACAGC TAGTGGAAAA ATTAAAAAAG GAATGGAATG TTATTTGTAT TACTGATGTT
GTCTACAATC ATACTGCTGC TAATAGTAAA TGGATCCAGG AACATCCAGA ATGTGCCTAT
AATCTTGTGA ATTCTCCACA CTTAAAACCT GCCTGGGTCT TAGACAGAGC ACTTTGGCGT
TTCTCCTGTG ATGTTGCAGA AGGGAAATAC AAAGAAAAGG GAATACCTGC TTTGATTGAA
AATGATCACC ATATGAATTC CATCCGAAAA ATAATTTGGG AGGATATTTT TCCAAAGCTT
AAACTCTGGG AATTTTTCCA AGTAGATGTC AACAAAGCGG TTGAGCAATT TAGAAGACTT
CTTACACAAG AAAATAGGCG AGTAACCAAG TCTGATCCAA ACCAACACCT TACGATTATT
CAAGATCCTG AATACAGACG GTTTGGCTGT ACTGTAGATA TGAACATTGC ACTAACGACT
TTCATACCAC ATGACAAGGG GCCAGCAGCA ATTGAAGAAT GCTGTAATTG GTTTCATAAA
AGAATGGAGG AATTAAATTC AGAGAAGCAT CGACTCATTA ACTATCATCA GGAACAGGCA
GTTAATTGCC TTTTGGGAAA TGTGTTTTAT GAACGACTGG CTGGCCATGG TCCAAAACTA
GGACCTGTCA CTAGAAAGCA TCCTTTAGTT ACCAGGTATT TTACTTTCCC ATTTGAAGAG
ATAGACTTCT CCATGGAAGA ATCTATGATT CATCTGCCAA ATAAAGCTTG TTTTCTGATG
GCACACAATG GATGGGTAAT GGGAGATGAT CCTCTTCGAA ACTTTGCTGA ACCGGGTTCA
GAAGTTTACC TAAGGAGAGA ACTTATTTGC TGGGGAGACA GTGTTAAATT ACGCTATGGG
AATAAACCAG AGGACTGTCC TTATCTCTGG GCACACATGA AAAAATACAC TGAAATAACT
GCAACTTATT TCCAGGGAGT ACGTCTTGAT AACTGCCACT CAACACCTCT TCACGTAGCT
GAGTACATGT TGGATGCTGC TAGGAATTTG CAACCCAATT TATATGTAGT AGCTGAACTG
TTCACAGGAA GTGAAGATCT GGACAATGTC TTTGTTACTA GACTGGGCAT TAGTTCCTTA
ATAAGAGAGG CAATGAGTGC ATATAATAGT CATGAAGAGG GCAGATTAGT TTACCGATAT
GGAGGAGAAC CTGTTGGATC CTTTGTTCAG CCCTGTTTGA GGCCTTTAAT GCCAGCTATT
GCACATGCCC TGTTTATGGA TATTACGCAT GATAATGAGT GTCCTATTGT GCATAGATCA
GCGTATGATG CTCTTCCAAG TACTACAATT GTTTCTATGG CATGTTGTGC TAGTGGAAGT
ACAAGAGGCT ATGATGAATT AGTGCCTCAT CAGATTTCAG TGGTTTCTGA AGAACGGTTT
TACACTAAGT GGAATCCTGA AGCATTGCCT TCAAACACAG GTGAAGTTAA TTTCCAAAGC
GGCATTATTG CAGCCAGGTG TGCTATCAGT AAACTTCATC AGGAGCTTGG AGCCAAGGGT
TTTATTCAGG TGTATGTGGA TCAAGTTGAT GAAGACATAG TGGCAGTAAC AAGACACTCA
CCTAGCATCC ATCAGTCTGT TGTGGCTGTA TCTAGAACTG CTTTCAGGAA TCCCAAGACT
TCATTTTACA GCAAGGAAGT GCCTCAAATG TGCATCCCTG GCAAAATTGA AGAAGTAGTT
CTTGAAGCTA GAACTATTGA GAGAAACACG AAACCTTATA GGAAGGATGA GAATTCAATC
AATGGAACAC CAGATATCAC AGTAGAAATT AGAGAACATA TTCAGCTTAA TGAAAGTAAA
ATTGTTAAAC AAGCTGGAGT TGCCACAAAA GGGCCCAATG AATATATTCA AGAAATAGAA
TTTGAAAACT TGTCTCCAGG AAGTGTTATT ATATTCAGAG TTAGTCTTGA TCCACATGCA
CAAGTCGCTG TTGGAATTCT TCGAAATCAT CTGACACAAT TCAGTCCTCA CTTTAAATCT
GGCAGCCTAG CTGTTGACAA TGCAGATCCT ATATTAAAAA TTCCTTTTGC TTCTCTTGCC
TCCAGATTAA CTTTGGCTGA GCTAAATCAG ATCCTTTACC GATGTGAATC AGAAGAAAAG
GAAGATGGTG GAGGGTGCTA TGACATACCA AACTGGTCAG CCCTTAAATA TGCAGGTCTT
CAAGGTTTAA TGTCTGTATT GGCAGAAATA AGACCAAAGA ATGACTTGGG GCATCCTTTT
TGTAATAATT TGAGATCTGG AGATTGGATG ATTGACTATG TCAGTAACCG GCTTATTTCA
CGATCAGGAA CTATTGCTGA AGTTGGTAAA TGGTTGCAGG CTATGTTCTT CTACCTGAAG
CAGATCCCAC GTTACCTTAT CCCATGTTAC TTTGATGCTA TATTAATTGG TGCATATACC
ACTCTTCTGG ATACAGCATG GAAGCAGATG TCAAGCTTTG TTCAGAATGG TTCAACCTTT
GTGAAACACC TTTCATTGGG TTCAGTTCAA CTGTGTGGAG TAGGAAAATT CCCTTCCCTG
CCAATTCTTT CACCTGCCCT AATGGATGTA CCTTATAGGT TAAATGAGAT CACAAAAGAA
AAGGAGCAAT GTTGTGTTTC TCTAGCTGCA GGCTTACCTC ATTTTTCTTC TGGTATTTTC
CGCTGCTGGG GAAGGGATAC TTTTATTGCA CTTAGAGGTA TACTGCTGAT TACTGGACGC
TATGTAGAAG CCAGGAATAT TATTTTAGCA TTTGCGGGTA CCCTGAGGCA TGGTCTCATT
CCTAATCTAC TGGGTGAAGG AATTTATGCC AGATACAATT GTCGGGATGC TGTGTGGTGG
TGGCTGCAGT GTATCCAGGA TTACTGTAAA ATGGTTCCAA ATGGTCTAGA CATTCTCAAG
TGCCCAGTTT CCAGAATGTA TCCTACAGAT GATTCTGCTC CTTTGCCTGC TGGCACACTG
GATCAGCCAT TGTTTGAAGT CATACAGGAA GCAATGCAAA AACACATGCA GGGCATACAG
TTCCGAGAAA GGAATGCTGG TCCCCAGATA GATCGAAACA TGAAGGACGA AGGTTTTAAT
ATAACTGCAG GAGTTGATGA AGAAACAGGA TTTGTTTATG GAGGAAATCG TTTCAATTGT
GGCACATGGA TGGATAAAAT GGGAGAAAGT GACAGAGCTA GAAACAGAGG AATCCCAGCC
ACACCAAGAG ATGGGTCTGC TGTGGAAATT GTGGGCCTGA GTAAATCTGC TGTTCGCTGG
TTGCTGGAAT TATCCAAAAA AAATATTTTC CCTTATCATG AAGTCACAGT AAAAAGACAT
GGAAAGGCTA TAAAGGTCTC ATATGATGAG TGGAACAGAA AAATACAAGA CAACTTTGAA
AAGCTATTTC ATGTTTCCGA AGACCCTTCA GATTTAAATG AAAAGCATCC AAATCTGGTT
CACAAACGTG GCATATACAA AGATAGTTAT GGAGCTTCAA GTCCTTGGTG TGACTATCAG
CTCAGGCCTA ATTTTACCAT AGCAATGGTT GTGGCCCCTG AGCTCTTTAC TACAGAAAAA
GCATGGAAAG CTTTGGAGAT TGCAGAAAAA AAATTGCTTG GTCCCCTTGG CATGAAAACT
TTAGATCCAG ATGATATGGT TTACTGTGGA ATTTATGACA ATGCATTAGA CAATGACAAC
TACAATCTTG CTAAAGGTTT CAATTATCAC CAAGGACCTG AGTGGCTGTG GCCTATTGGG
TATTTTCTTC GTGCAAAATT ATATTTTTCC AGATTGATGG GCCCGGAGAC TACTGCAAAG
ACTATAGTTT TGGTTAAAAA TGTTCTTTCC CGACATTATG TTCATCTTGA GAGATCCCCT
TGGAAAGGAC TTCCAGAACT GACCAATGAG AATGCCCAGT ACTGTCCTTT CAGCTGTGAA
ACACAAGCCT GGTCAATTGC TACTATTCTT GAGACACTTT ATGATTTATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000637.2
CDS260..4810
Misc Feature(1)224..226(+)
Misc Feature(2)290..4807(+)
Misc Feature(3)299..562(+)
Misc Feature(4)524..1960(+)
Misc Feature(5)1961..1963(+)
Misc Feature(6)2300..3133(+)
Misc Feature(7)3428..4792(+)
Exon (1)1..150
Gene:AGL
Gene Synonym:
Exon (2)151..293
Gene:AGL
Gene Synonym:
Exon (3)294..504
Gene:AGL
Gene Synonym:
Exon (4)505..671
Gene:AGL
Gene Synonym:
Exon (5)672..875
Gene:AGL
Gene Synonym:
Exon (6)876..1057
Gene:AGL
Gene Synonym:
Exon (7)1058..1169
Gene:AGL
Gene Synonym:
Exon (8)1170..1293
Gene:AGL
Gene Synonym:
Exon (9)1294..1396
Gene:AGL
Gene Synonym:
Exon (10)1397..1494
Gene:AGL
Gene Synonym:
Exon (11)1495..1634
Gene:AGL
Gene Synonym:
Exon (12)1635..1822
Gene:AGL
Gene Synonym:
Exon (13)1823..1946
Gene:AGL
Gene Synonym:
Exon (14)1947..2110
Gene:AGL
Gene Synonym:
Exon (15)2111..2212
Gene:AGL
Gene Synonym:
Exon (16)2213..2368
Gene:AGL
Gene Synonym:
Exon (17)2369..2519
Gene:AGL
Gene Synonym:
Exon (18)2520..2644
Gene:AGL
Gene Synonym:
Exon (19)2645..2757
Gene:AGL
Gene Synonym:
Exon (20)2758..2892
Gene:AGL
Gene Synonym:
Exon (21)2893..3023
Gene:AGL
Gene Synonym:
Exon (22)3024..3160
Gene:AGL
Gene Synonym:
Exon (23)3161..3294
Gene:AGL
Gene Synonym:
Exon (24)3295..3470
Gene:AGL
Gene Synonym:
Exon (25)3471..3573
Gene:AGL
Gene Synonym:
Exon (26)3574..3799
Gene:AGL
Gene Synonym:
Exon (27)3800..3911
Gene:AGL
Gene Synonym:
Exon (28)3912..4047
Gene:AGL
Gene Synonym:
Exon (29)4048..4160
Gene:AGL
Gene Synonym:
Exon (30)4161..4372
Gene:AGL
Gene Synonym:
Exon (31)4373..4470
Gene:AGL
Gene Synonym:
Exon (32)4471..4558
Gene:AGL
Gene Synonym:
Exon (33)4559..4692
Gene:AGL
Gene Synonym:
Exon (34)4693..7182
Gene:AGL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000646
Organism Homo sapiens (human)
Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000646

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGGCCCCAA TTCTGAGCAT TAATTTATTT ATTGGGTATG AGCTACAGTT CCGATTAGGC 
CCAACTTTAC AGGGAAAAGC AGTTACCGTG TATACAAATT ACCCATTTCC TGGAGAAACA
TTTAATAGAG AAAAATTCCG TTCTCTGGAT TGGGAAAATC CAACAGAAAG AGAAGATGAT
TCTGATAAAT ACTGTAAACT TAATCTGCAA CAATCTGGTT CATTTCAGTA TTATTTCCTT
CAAGGAAATG AGAAAAGTGG TGGAGGTTAC ATAGTTGTGG ACCCCATTTT ACGTGTTGGT
GCTGATAATC ATGTGCTACC CTTGGACTGT GTTACTCTTC AGACATTTTT AGCTAAGTGT
TTGGGACCTT TTGATGAATG GGAAAGCAGA CTTAGGGTTG CAAAAGAATC AGGCTACAAC
ATGATTCATT TTACCCCATT GCAGACTCTT GGACTATCTA GGTCATGCTA CTCCCTTGCC
AATCAGTTAG AATTAAATCC TGACTTTTCA AGACCTAATA GAAAGTATAC CTGGAATGAT
GTTGGACAGC TAGTGGAAAA ATTAAAAAAG GAATGGAATG TTATTTGTAT TACTGATGTT
GTCTACAATC ATACTGCTGC TAATAGTAAA TGGATCCAGG AACATCCAGA ATGTGCCTAT
AATCTTGTGA ATTCTCCACA CTTAAAACCT GCCTGGGTCT TAGACAGAGC ACTTTGGCGT
TTCTCCTGTG ATGTTGCAGA AGGGAAATAC AAAGAAAAGG GAATACCTGC TTTGATTGAA
AATGATCACC ATATGAATTC CATCCGAAAA ATAATTTGGG AGGATATTTT TCCAAAGCTT
AAACTCTGGG AATTTTTCCA AGTAGATGTC AACAAAGCGG TTGAGCAATT TAGAAGACTT
CTTACACAAG AAAATAGGCG AGTAACCAAG TCTGATCCAA ACCAACACCT TACGATTATT
CAAGATCCTG AATACAGACG GTTTGGCTGT ACTGTAGATA TGAACATTGC ACTAACGACT
TTCATACCAC ATGACAAGGG GCCAGCAGCA ATTGAAGAAT GCTGTAATTG GTTTCATAAA
AGAATGGAGG AATTAAATTC AGAGAAGCAT CGACTCATTA ACTATCATCA GGAACAGGCA
GTTAATTGCC TTTTGGGAAA TGTGTTTTAT GAACGACTGG CTGGCCATGG TCCAAAACTA
GGACCTGTCA CTAGAAAGCA TCCTTTAGTT ACCAGGTATT TTACTTTCCC ATTTGAAGAG
ATAGACTTCT CCATGGAAGA ATCTATGATT CATCTGCCAA ATAAAGCTTG TTTTCTGATG
GCACACAATG GATGGGTAAT GGGAGATGAT CCTCTTCGAA ACTTTGCTGA ACCGGGTTCA
GAAGTTTACC TAAGGAGAGA ACTTATTTGC TGGGGAGACA GTGTTAAATT ACGCTATGGG
AATAAACCAG AGGACTGTCC TTATCTCTGG GCACACATGA AAAAATACAC TGAAATAACT
GCAACTTATT TCCAGGGAGT ACGTCTTGAT AACTGCCACT CAACACCTCT TCACGTAGCT
GAGTACATGT TGGATGCTGC TAGGAATTTG CAACCCAATT TATATGTAGT AGCTGAACTG
TTCACAGGAA GTGAAGATCT GGACAATGTC TTTGTTACTA GACTGGGCAT TAGTTCCTTA
ATAAGAGAGG CAATGAGTGC ATATAATAGT CATGAAGAGG GCAGATTAGT TTACCGATAT
GGAGGAGAAC CTGTTGGATC CTTTGTTCAG CCCTGTTTGA GGCCTTTAAT GCCAGCTATT
GCACATGCCC TGTTTATGGA TATTACGCAT GATAATGAGT GTCCTATTGT GCATAGATCA
GCGTATGATG CTCTTCCAAG TACTACAATT GTTTCTATGG CATGTTGTGC TAGTGGAAGT
ACAAGAGGCT ATGATGAATT AGTGCCTCAT CAGATTTCAG TGGTTTCTGA AGAACGGTTT
TACACTAAGT GGAATCCTGA AGCATTGCCT TCAAACACAG GTGAAGTTAA TTTCCAAAGC
GGCATTATTG CAGCCAGGTG TGCTATCAGT AAACTTCATC AGGAGCTTGG AGCCAAGGGT
TTTATTCAGG TGTATGTGGA TCAAGTTGAT GAAGACATAG TGGCAGTAAC AAGACACTCA
CCTAGCATCC ATCAGTCTGT TGTGGCTGTA TCTAGAACTG CTTTCAGGAA TCCCAAGACT
TCATTTTACA GCAAGGAAGT GCCTCAAATG TGCATCCCTG GCAAAATTGA AGAAGTAGTT
CTTGAAGCTA GAACTATTGA GAGAAACACG AAACCTTATA GGAAGGATGA GAATTCAATC
AATGGAACAC CAGATATCAC AGTAGAAATT AGAGAACATA TTCAGCTTAA TGAAAGTAAA
ATTGTTAAAC AAGCTGGAGT TGCCACAAAA GGGCCCAATG AATATATTCA AGAAATAGAA
TTTGAAAACT TGTCTCCAGG AAGTGTTATT ATATTCAGAG TTAGTCTTGA TCCACATGCA
CAAGTCGCTG TTGGAATTCT TCGAAATCAT CTGACACAAT TCAGTCCTCA CTTTAAATCT
GGCAGCCTAG CTGTTGACAA TGCAGATCCT ATATTAAAAA TTCCTTTTGC TTCTCTTGCC
TCCAGATTAA CTTTGGCTGA GCTAAATCAG ATCCTTTACC GATGTGAATC AGAAGAAAAG
GAAGATGGTG GAGGGTGCTA TGACATACCA AACTGGTCAG CCCTTAAATA TGCAGGTCTT
CAAGGTTTAA TGTCTGTATT GGCAGAAATA AGACCAAAGA ATGACTTGGG GCATCCTTTT
TGTAATAATT TGAGATCTGG AGATTGGATG ATTGACTATG TCAGTAACCG GCTTATTTCA
CGATCAGGAA CTATTGCTGA AGTTGGTAAA TGGTTGCAGG CTATGTTCTT CTACCTGAAG
CAGATCCCAC GTTACCTTAT CCCATGTTAC TTTGATGCTA TATTAATTGG TGCATATACC
ACTCTTCTGG ATACAGCATG GAAGCAGATG TCAAGCTTTG TTCAGAATGG TTCAACCTTT
GTGAAACACC TTTCATTGGG TTCAGTTCAA CTGTGTGGAG TAGGAAAATT CCCTTCCCTG
CCAATTCTTT CACCTGCCCT AATGGATGTA CCTTATAGGT TAAATGAGAT CACAAAAGAA
AAGGAGCAAT GTTGTGTTTC TCTAGCTGCA GGCTTACCTC ATTTTTCTTC TGGTATTTTC
CGCTGCTGGG GAAGGGATAC TTTTATTGCA CTTAGAGGTA TACTGCTGAT TACTGGACGC
TATGTAGAAG CCAGGAATAT TATTTTAGCA TTTGCGGGTA CCCTGAGGCA TGGTCTCATT
CCTAATCTAC TGGGTGAAGG AATTTATGCC AGATACAATT GTCGGGATGC TGTGTGGTGG
TGGCTGCAGT GTATCCAGGA TTACTGTAAA ATGGTTCCAA ATGGTCTAGA CATTCTCAAG
TGCCCAGTTT CCAGAATGTA TCCTACAGAT GATTCTGCTC CTTTGCCTGC TGGCACACTG
GATCAGCCAT TGTTTGAAGT CATACAGGAA GCAATGCAAA AACACATGCA GGGCATACAG
TTCCGAGAAA GGAATGCTGG TCCCCAGATA GATCGAAACA TGAAGGACGA AGGTTTTAAT
ATAACTGCAG GAGTTGATGA AGAAACAGGA TTTGTTTATG GAGGAAATCG TTTCAATTGT
GGCACATGGA TGGATAAAAT GGGAGAAAGT GACAGAGCTA GAAACAGAGG AATCCCAGCC
ACACCAAGAG ATGGGTCTGC TGTGGAAATT GTGGGCCTGA GTAAATCTGC TGTTCGCTGG
TTGCTGGAAT TATCCAAAAA AAATATTTTC CCTTATCATG AAGTCACAGT AAAAAGACAT
GGAAAGGCTA TAAAGGTCTC ATATGATGAG TGGAACAGAA AAATACAAGA CAACTTTGAA
AAGCTATTTC ATGTTTCCGA AGACCCTTCA GATTTAAATG AAAAGCATCC AAATCTGGTT
CACAAACGTG GCATATACAA AGATAGTTAT GGAGCTTCAA GTCCTTGGTG TGACTATCAG
CTCAGGCCTA ATTTTACCAT AGCAATGGTT GTGGCCCCTG AGCTCTTTAC TACAGAAAAA
GCATGGAAAG CTTTGGAGAT TGCAGAAAAA AAATTGCTTG GTCCCCTTGG CATGAAAACT
TTAGATCCAG ATGATATGGT TTACTGTGGA ATTTATGACA ATGCATTAGA CAATGACAAC
TACAATCTTG CTAAAGGTTT CAATTATCAC CAAGGACCTG AGTGGCTGTG GCCTATTGGG
TATTTTCTTC GTGCAAAATT ATATTTTTCC AGATTGATGG GCCCGGAGAC TACTGCAAAG
ACTATAGTTT TGGTTAAAAA TGTTCTTTCC CGACATTATG TTCATCTTGA GAGATCCCCT
TGGAAAGGAC TTCCAGAACT GACCAATGAG AATGCCCAGT ACTGTCCTTT CAGCTGTGAA
ACACAAGCCT GGTCAATTGC TACTATTCTT GAGACACTTT ATGATTTATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16218
Clone ID Related Accession (Same CDS sequence) NM_000028 , NM_000642 , NM_000643 , NM_000644 , XM_005270557
Accession Version NM_000643.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glycogen debranching enzyme isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84009.1 and AC096949.2. On Oct 27, 2006 this sequence version replaced gi:4557278. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84009.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2151358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000634.2
CDS199..4797
Misc Feature(1)169..171(+)
Misc Feature(2)262..4794(+)
Misc Feature(3)286..549(+)
Misc Feature(4)511..1947(+)
Misc Feature(5)1948..1950(+)
Misc Feature(6)2287..3120(+)
Misc Feature(7)3415..4779(+)
Exon (1)1..130
Gene:AGL
Gene Synonym:
Exon (2)131..280
Gene:AGL
Gene Synonym:
Exon (3)281..491
Gene:AGL
Gene Synonym:
Exon (4)492..658
Gene:AGL
Gene Synonym:
Exon (5)659..862
Gene:AGL
Gene Synonym:
Exon (6)863..1044
Gene:AGL
Gene Synonym:
Exon (7)1045..1156
Gene:AGL
Gene Synonym:
Exon (8)1157..1280
Gene:AGL
Gene Synonym:
Exon (9)1281..1383
Gene:AGL
Gene Synonym:
Exon (10)1384..1481
Gene:AGL
Gene Synonym:
Exon (11)1482..1621
Gene:AGL
Gene Synonym:
Exon (12)1622..1809
Gene:AGL
Gene Synonym:
Exon (13)1810..1933
Gene:AGL
Gene Synonym:
Exon (14)1934..2097
Gene:AGL
Gene Synonym:
Exon (15)2098..2199
Gene:AGL
Gene Synonym:
Exon (16)2200..2355
Gene:AGL
Gene Synonym:
Exon (17)2356..2506
Gene:AGL
Gene Synonym:
Exon (18)2507..2631
Gene:AGL
Gene Synonym:
Exon (19)2632..2744
Gene:AGL
Gene Synonym:
Exon (20)2745..2879
Gene:AGL
Gene Synonym:
Exon (21)2880..3010
Gene:AGL
Gene Synonym:
Exon (22)3011..3147
Gene:AGL
Gene Synonym:
Exon (23)3148..3281
Gene:AGL
Gene Synonym:
Exon (24)3282..3457
Gene:AGL
Gene Synonym:
Exon (25)3458..3560
Gene:AGL
Gene Synonym:
Exon (26)3561..3786
Gene:AGL
Gene Synonym:
Exon (27)3787..3898
Gene:AGL
Gene Synonym:
Exon (28)3899..4034
Gene:AGL
Gene Synonym:
Exon (29)4035..4147
Gene:AGL
Gene Synonym:
Exon (30)4148..4359
Gene:AGL
Gene Synonym:
Exon (31)4360..4457
Gene:AGL
Gene Synonym:
Exon (32)4458..4545
Gene:AGL
Gene Synonym:
Exon (33)4546..4679
Gene:AGL
Gene Synonym:
Exon (34)4680..7169
Gene:AGL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000643
Organism Homo sapiens (human)
Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000643

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16218
Clone ID Related Accession (Same CDS sequence) NM_000028 , NM_000642 , NM_000643 , NM_000644 , XM_005270557
Accession Version NM_000644.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glycogen debranching enzyme isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U84008.1 and AC096949.2. On Oct 27, 2006 this sequence version replaced gi:4557280. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155550 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000635.2
CDS139..4737
Misc Feature(1)130..132(+)
Misc Feature(2)202..4734(+)
Misc Feature(3)226..489(+)
Misc Feature(4)451..1887(+)
Misc Feature(5)1888..1890(+)
Misc Feature(6)2227..3060(+)
Misc Feature(7)3355..4719(+)
Exon (1)1..130
Gene:AGL
Gene Synonym:
Exon (2)131..220
Gene:AGL
Gene Synonym:
Exon (3)221..431
Gene:AGL
Gene Synonym:
Exon (4)432..598
Gene:AGL
Gene Synonym:
Exon (5)599..802
Gene:AGL
Gene Synonym:
Exon (6)803..984
Gene:AGL
Gene Synonym:
Exon (7)985..1096
Gene:AGL
Gene Synonym:
Exon (8)1097..1220
Gene:AGL
Gene Synonym:
Exon (9)1221..1323
Gene:AGL
Gene Synonym:
Exon (10)1324..1421
Gene:AGL
Gene Synonym:
Exon (11)1422..1561
Gene:AGL
Gene Synonym:
Exon (12)1562..1749
Gene:AGL
Gene Synonym:
Exon (13)1750..1873
Gene:AGL
Gene Synonym:
Exon (14)1874..2037
Gene:AGL
Gene Synonym:
Exon (15)2038..2139
Gene:AGL
Gene Synonym:
Exon (16)2140..2295
Gene:AGL
Gene Synonym:
Exon (17)2296..2446
Gene:AGL
Gene Synonym:
Exon (18)2447..2571
Gene:AGL
Gene Synonym:
Exon (19)2572..2684
Gene:AGL
Gene Synonym:
Exon (20)2685..2819
Gene:AGL
Gene Synonym:
Exon (21)2820..2950
Gene:AGL
Gene Synonym:
Exon (22)2951..3087
Gene:AGL
Gene Synonym:
Exon (23)3088..3221
Gene:AGL
Gene Synonym:
Exon (24)3222..3397
Gene:AGL
Gene Synonym:
Exon (25)3398..3500
Gene:AGL
Gene Synonym:
Exon (26)3501..3726
Gene:AGL
Gene Synonym:
Exon (27)3727..3838
Gene:AGL
Gene Synonym:
Exon (28)3839..3974
Gene:AGL
Gene Synonym:
Exon (29)3975..4087
Gene:AGL
Gene Synonym:
Exon (30)4088..4299
Gene:AGL
Gene Synonym:
Exon (31)4300..4397
Gene:AGL
Gene Synonym:
Exon (32)4398..4485
Gene:AGL
Gene Synonym:
Exon (33)4486..4619
Gene:AGL
Gene Synonym:
Exon (34)4620..7109
Gene:AGL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000644
Organism Homo sapiens (human)
Definition Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000644

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGGGACACA GTAAACAGAT TCGAATTTTA CTTCTGAACG AAATGGAGAA ACTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGGTATGAG CTACAGTTCC GATTAGGCCC AACTTTACAG
GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT TAATAGAGAA
AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC
TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT ATTTCCTTCA AGGAAATGAG
AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC GTGTTGGTGC TGATAATCAT
GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG CTAAGTGTTT GGGACCTTTT
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCATTTT
ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA
TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT GGAATGATGT TGGACAGCTA
GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA CTGATGTTGT CTACAATCAT
ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT GTGCCTATAA TCTTGTGAAT
TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TTTGGCGTTT CTCCTGTGAT
GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT TGATTGAAAA TGATCACCAT
ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC CAAAGCTTAA ACTCTGGGAA
TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA GAAGACTTCT TACACAAGAA
AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA CGATTATTCA AGATCCTGAA
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAACGACTTT CATACCACAT
GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT TTCATAAAAG AATGGAGGAA
TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG AACAGGCAGT TAATTGCCTT
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CAAAACTAGG ACCTGTCACT
AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT TTGAAGAGAT AGACTTCTCC
ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT TTCTGATGGC ACACAATGGA
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CGGGTTCAGA AGTTTACCTA
AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG
GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG AAATAACTGC AACTTATTTC
CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC ACGTAGCTGA GTACATGTTG
GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG CTGAACTGTT CACAGGAAGT
GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG AGGAGAACCT
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CAGCTATTGC ACATGCCCTG
TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC ATAGATCAGC GTATGATGCT
CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA GTGGAAGTAC AAGAGGCTAT
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG
AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT TCCAAAGCGG CATTATTGCA
GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG CCAAGGGTTT TATTCAGGTG
TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA GACACTCACC TAGCATCCAT
CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC
AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA
ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAACACCA
GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAAAAT TGTTAAACAA
GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTCGCTGTT
GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT TTAAATCTGG CAGCCTAGCT
GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT CTCTTGCCTC CAGATTAACT
TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG AAGAAAAGGA AGATGGTGGA
GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG CAGGTCTTCA AGGTTTAATG
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TAATAATTTG
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCAGGAACT
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT
TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG CATATACCAC TCTTCTGGAT
ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACCTTTGT GAAACACCTT
TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC CTTCCCTGCC AATTCTTTCA
CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA CAAAAGAAAA GGAGCAATGT
TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTCCG CTGCTGGGGA
AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA CTGGACGCTA TGTAGAAGCC
AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG GTCTCATTCC TAATCTACTG
GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG TGTGGTGGTG GCTGCAGTGT
ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TTCTCAAGTG CCCAGTTTCC
AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG
TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG GCATACAGTT CCGAGAAAGG
AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG GTTTTAATAT AACTGCAGGA
GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT TCAATTGTGG CACATGGATG
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC ACCAAGAGAT
GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG TTCGCTGGTT GCTGGAATTA
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGCTATA
AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA ACTTTGAAAA GCTATTTCAT
GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA ATCTGGTTCA CAAACGTGGC
ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG ACTATCAGCT CAGGCCTAAT
TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA CAGAAAAAGC ATGGAAAGCT
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA TGAAAACTTT AGATCCAGAT
GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA ATGACAACTA CAATCTTGCT
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT
GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA CTGCAAAGAC TATAGTTTTG
GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCCCTTG GAAAGGACTT
CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG
TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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