EGF cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following EGF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23582 NM_001963.6
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu94958 XM_017007853.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu94959 XM_017007854.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu94960 XM_017007855.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu94957 NM_001357021.2
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu94950 XM_017007845.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu94951 XM_017007846.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu94952 XM_017007847.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu94953 XM_017007848.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu94954 XM_017007849.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu94955 XM_017007850.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu94956 XM_017007851.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu94957 XM_017007852.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu15827 NM_001178131.3
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $825.30
$1179.00
OHu16028 NM_001178130.3
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $825.30
$1179.00
OHu38745 XM_005262796.2
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu57444 XM_011531707.1
Latest version!
Homo sapiens epidermal growth factor (EGF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OHu23582 NM_001963.5 Homo sapiens epidermal growth factor (EGF), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu16028 NM_001178130.2 Homo sapiens epidermal growth factor (EGF), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $825.30
$1179.00
OHu94957 NM_001357021.1 Homo sapiens epidermal growth factor (EGF), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu15827 NM_001178131.2 Homo sapiens epidermal growth factor (EGF), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $825.30
$1179.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu23582
    Clone ID Related Accession (Same CDS sequence) NM_001963.6 , NM_001963.5
    Accession Version NM_001963.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, BC093731.1, AC004050.1, AK299306.1 and CA437074.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001963.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X04571.1, BC093731.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265171.10/ ENSP00000265171.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG
    AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG
    TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT
    GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC
    ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC
    TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA
    TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC
    TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA
    TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA
    CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA
    ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC
    CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT
    ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT
    GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA
    AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA
    CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT
    CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT
    CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT
    GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC
    CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA
    GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT
    CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA
    GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT
    GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT
    CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA
    ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA
    CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC
    AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA
    TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG
    TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA
    GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG
    CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT
    GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC
    AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC
    GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA
    TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG
    GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG
    GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG
    AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT
    CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT
    ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG
    GCAGCAGATG GGTCAATGCA ACCAACTTCA TGGAGGCAGG AGCCCCAGTT ATGTGGAATG
    GGCACAGAGC AAGGCTGCTG GATTCCAGTA TCCAGTGATA AGGGCTCCTG TCCCCAGGTA
    ATGGAGCGAA GCTTTCATAT GCCCTCCTAT GGGACACAGA CCCTTGAAGG GGGTGTCGAG
    AAGCCCCATT CTCTCCTATC AGCTAACCCA TTATGGCAAC AAAGGGCCCT GGACCCACCA
    CACCAAATGG AGCTGACTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001954.2
    CDS454..4077
    Translation

    Target ORF information:

    RefSeq Version NM_001963.6
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001963.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG
    AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG
    TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT
    GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC
    ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC
    TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA
    TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC
    TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA
    TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA
    CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA
    ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC
    CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT
    ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT
    GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA
    AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA
    CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT
    CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT
    CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT
    GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC
    CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA
    GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT
    CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA
    GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT
    GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT
    CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA
    ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA
    CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC
    AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA
    TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG
    TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA
    GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG
    CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT
    GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC
    AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC
    GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA
    TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG
    GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG
    GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG
    AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT
    CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT
    ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG
    GCAGCAGATG GGTCAATGCA ACCAACTTCA TGGAGGCAGG AGCCCCAGTT ATGTGGAATG
    GGCACAGAGC AAGGCTGCTG GATTCCAGTA TCCAGTGATA AGGGCTCCTG TCCCCAGGTA
    ATGGAGCGAA GCTTTCATAT GCCCTCCTAT GGGACACAGA CCCTTGAAGG GGGTGTCGAG
    AAGCCCCATT CTCTCCTATC AGCTAACCCA TTATGGCAAC AAAGGGCCCT GGACCCACCA
    CACCAAATGG AGCTGACTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23582
    Clone ID Related Accession (Same CDS sequence) NM_001963.6 , NM_001963.5
    Accession Version NM_001963.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, BC093731.1, AC004050.1, AK299306.1 and CA437074.1. This sequence is a reference standard in the RefSeqGene project. On Jan 8, 2016 this sequence version replaced NM_001963.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X04571.1, BC093731.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG
    AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG
    TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT
    GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC
    ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC
    TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA
    TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC
    TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA
    TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA
    CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA
    ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC
    CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT
    ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT
    GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA
    AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA
    CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT
    CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT
    CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT
    GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC
    CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA
    GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT
    CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA
    GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT
    GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT
    CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA
    ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA
    CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC
    AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA
    TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG
    TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA
    GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG
    CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT
    GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC
    AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC
    GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA
    TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG
    GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG
    GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG
    AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT
    CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT
    ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG
    GCAGCAGATG GGTCAATGCA ACCAACTTCA TGGAGGCAGG AGCCCCAGTT ATGTGGAATG
    GGCACAGAGC AAGGCTGCTG GATTCCAGTA TCCAGTGATA AGGGCTCCTG TCCCCAGGTA
    ATGGAGCGAA GCTTTCATAT GCCCTCCTAT GGGACACAGA CCCTTGAAGG GGGTGTCGAG
    AAGCCCCATT CTCTCCTATC AGCTAACCCA TTATGGCAAC AAAGGGCCCT GGACCCACCA
    CACCAAATGG AGCTGACTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001954.2
    CDS453..4076
    Translation

    Target ORF information:

    RefSeq Version NM_001963.5
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001963.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG
    AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG
    TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT
    GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC
    ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC
    TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA
    TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC
    TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA
    TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA
    CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA
    ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC
    CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT
    ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT
    GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA
    AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA
    CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT
    CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT
    CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT
    GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC
    CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA
    GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT
    CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA
    GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT
    GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT
    CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA
    ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA
    CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC
    AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA
    TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG
    TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA
    GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG
    CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT
    GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC
    AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC
    GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA
    TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG
    GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG
    GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG
    AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT
    CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT
    ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG
    GCAGCAGATG GGTCAATGCA ACCAACTTCA TGGAGGCAGG AGCCCCAGTT ATGTGGAATG
    GGCACAGAGC AAGGCTGCTG GATTCCAGTA TCCAGTGATA AGGGCTCCTG TCCCCAGGTA
    ATGGAGCGAA GCTTTCATAT GCCCTCCTAT GGGACACAGA CCCTTGAAGG GGGTGTCGAG
    AAGCCCCATT CTCTCCTATC AGCTAACCCA TTATGGCAAC AAAGGGCCCT GGACCCACCA
    CACCAAATGG AGCTGACTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94958
    Clone ID Related Accession (Same CDS sequence) XM_017007853.1
    Accession Version XM_017007853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA
    GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT
    GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT
    GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA
    GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGCT
    GTGTTGTTGG CTACATCGGG GAGCGATGTC AGTACCGAGA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863342.1
    CDS454..3258
    Translation

    Target ORF information:

    RefSeq Version XM_017007853.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA
    GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT
    GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT
    GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA
    GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGCT
    GTGTTGTTGG CTACATCGGG GAGCGATGTC AGTACCGAGA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94959
    Clone ID Related Accession (Same CDS sequence) XM_017007854.1
    Accession Version XM_017007854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGAAGAAG CTAACCAACT AATAATCTGT
    ATAAGATACC TATTTTGCCC ATTCTCTTTG GCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863343.1
    CDS454..2589
    Translation

    Target ORF information:

    RefSeq Version XM_017007854.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGAAGAAG CTAACCAACT AATAATCTGT
    ATAAGATACC TATTTTGCCC ATTCTCTTTG GCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94960
    Clone ID Related Accession (Same CDS sequence) XM_017007855.1
    Accession Version XM_017007855.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTCACCC ATTTGCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863344.1
    CDS454..2544
    Translation

    Target ORF information:

    RefSeq Version XM_017007855.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007855.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTCACCC ATTTGCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94957
    Clone ID Related Accession (Same CDS sequence) XM_017007852.1 , NM_001357021.2 , NM_001357021.1
    Accession Version NM_001357021.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, AK299306.1, AC004050.1 and CA437074.1. On May 31, 2019 this sequence version replaced NM_001357021.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.275536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT
    TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC
    CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA
    CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC
    TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC
    GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT
    TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA
    CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC
    TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG
    GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT
    GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG
    GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT
    GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA
    ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA
    ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC
    TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG
    CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT
    GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG
    GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA
    GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA
    GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT
    GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT
    CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA
    TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG
    CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT
    GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC
    CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT
    GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC
    TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT
    GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC
    CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT
    GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA
    TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT
    GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT
    CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG
    TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001343950.1
    CDS454..3486
    Translation

    Target ORF information:

    RefSeq Version NM_001357021.2
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001357021.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT
    TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC
    CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA
    CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC
    TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC
    GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT
    TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA
    CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC
    TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG
    GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT
    GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG
    GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT
    GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA
    ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA
    ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC
    TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG
    CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT
    GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG
    GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA
    GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA
    GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT
    GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT
    CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA
    TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG
    CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT
    GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC
    CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT
    GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC
    TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT
    GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC
    CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT
    GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA
    TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT
    GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT
    CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG
    TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94957
    Clone ID Related Accession (Same CDS sequence) XM_017007852.1 , NM_001357021.2 , NM_001357021.1
    Accession Version NM_001357021.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299306.1, AC005509.1, AC004050.1 and CA437074.1. On Oct 16, 2017 this sequence version replaced XM_017007852.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.275536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT
    TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC
    CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA
    CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC
    TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC
    GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT
    TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA
    CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC
    TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG
    GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT
    GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG
    GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT
    GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA
    ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA
    ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC
    TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG
    CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT
    GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG
    GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA
    GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA
    GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT
    GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT
    CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA
    TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG
    CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT
    GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC
    CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT
    GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC
    TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT
    GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC
    CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT
    GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA
    TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT
    GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT
    CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG
    TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001343950.1
    CDS453..3485
    Translation

    Target ORF information:

    RefSeq Version NM_001357021.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001357021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT
    TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC
    CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA
    CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC
    TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC
    GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT
    TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA
    CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC
    TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG
    GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT
    GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG
    GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT
    GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA
    ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA
    ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC
    TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG
    CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT
    GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG
    GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA
    GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA
    GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT
    GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT
    CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA
    TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG
    CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT
    GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC
    CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT
    GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC
    TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT
    GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC
    CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT
    GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA
    TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT
    GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT
    CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG
    TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94950
    Clone ID Related Accession (Same CDS sequence) XM_017007845.1
    Accession Version XM_017007845.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3672bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA
    GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT
    GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT
    GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA
    GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT
    ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA
    GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT
    GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT
    GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT
    ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT
    CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG
    GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG
    GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG
    GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT
    TGCCCTCAAC CTTGGTTTGT GGTTATAAAA GAACACCAAG ACCTCAAGAA TGGGGGTCAA
    CCAGTGGCTG GTGAGGATGG CCAGGCAGCA GATGGTCAGT TTTTATCCCT GGCTCCTGGG
    TCAATGCAAC CAACTTCATG GAGGCAGGAG CCCCAGTTAT GTGGAATGGG CACAGAGCAA
    GGCTGCTGGA TTCCAGTATC CAGTGATAAG GGCTCCTGTC CCCAGGTAAT GGAGCGAAGC
    TTTCATATGC CCTCCTATGG GACACAGACC CTTGAAGGGG GTGTCGAGAA GCCCCATTCT
    CTCCTATCAG CTAACCCATT ATGGCAACAA AGGGCCCTGG ACCCACCACA CCAAATGGAG
    CTGACTCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863334.1
    CDS454..4125
    Translation

    Target ORF information:

    RefSeq Version XM_017007845.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA
    GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT
    GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT
    GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA
    GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT
    ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA
    GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT
    GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT
    GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT
    ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT
    CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG
    GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG
    GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG
    GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT
    TGCCCTCAAC CTTGGTTTGT GGTTATAAAA GAACACCAAG ACCTCAAGAA TGGGGGTCAA
    CCAGTGGCTG GTGAGGATGG CCAGGCAGCA GATGGTCAGT TTTTATCCCT GGCTCCTGGG
    TCAATGCAAC CAACTTCATG GAGGCAGGAG CCCCAGTTAT GTGGAATGGG CACAGAGCAA
    GGCTGCTGGA TTCCAGTATC CAGTGATAAG GGCTCCTGTC CCCAGGTAAT GGAGCGAAGC
    TTTCATATGC CCTCCTATGG GACACAGACC CTTGAAGGGG GTGTCGAGAA GCCCCATTCT
    CTCCTATCAG CTAACCCATT ATGGCAACAA AGGGCCCTGG ACCCACCACA CCAAATGGAG
    CTGACTCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94951
    Clone ID Related Accession (Same CDS sequence) XM_017007846.1
    Accession Version XM_017007846.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA
    GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT
    GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT
    GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA
    GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT
    ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA
    GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT
    GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT
    GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT
    ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT
    CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG
    GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG
    GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG
    GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT
    TGCCCTCAAC CTTGGTTTGT GGTTATAAAA GAACACCAAG ACCTCAAGAA TGGGGGTCAA
    CCAGTGGCTG GTGAGGATGG CCAGGCAGCA GATGGGTCAA TGCAACCAAC TTCATGGAGG
    CAGGAGCCCC AGTTATGTGG AATGGGCACA GAGCAAGGCT GCTGGATTCC AGTATCCAGT
    GATAAGGGCT CCTGTCCCCA GGTAATGGAG CGAAGCTTTC ATATGCCCTC CTATGGGACA
    CAGACCCTTG AAGGGGGTGT CGAGAAGCCC CATTCTCTCC TATCAGCTAA CCCATTATGG
    CAACAAAGGG CCCTGGACCC ACCACACCAA ATGGAGCTGA CTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863335.1
    CDS454..4101
    Translation

    Target ORF information:

    RefSeq Version XM_017007846.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007846.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA
    GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT
    GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT
    GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA
    GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT
    ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA
    GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT
    GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT
    GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT
    ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT
    CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG
    GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG
    GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG
    GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT
    TGCCCTCAAC CTTGGTTTGT GGTTATAAAA GAACACCAAG ACCTCAAGAA TGGGGGTCAA
    CCAGTGGCTG GTGAGGATGG CCAGGCAGCA GATGGGTCAA TGCAACCAAC TTCATGGAGG
    CAGGAGCCCC AGTTATGTGG AATGGGCACA GAGCAAGGCT GCTGGATTCC AGTATCCAGT
    GATAAGGGCT CCTGTCCCCA GGTAATGGAG CGAAGCTTTC ATATGCCCTC CTATGGGACA
    CAGACCCTTG AAGGGGGTGT CGAGAAGCCC CATTCTCTCC TATCAGCTAA CCCATTATGG
    CAACAAAGGG CCCTGGACCC ACCACACCAA ATGGAGCTGA CTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94952
    Clone ID Related Accession (Same CDS sequence) XM_017007847.1
    Accession Version XM_017007847.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA
    GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT
    GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT
    GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA
    GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAC
    TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC
    TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT
    GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG
    TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC
    ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG
    GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG
    TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC
    CCTCAACCTT GGTTTGTGGT TATAAAAGAA CACCAAGACC TCAAGAATGG GGGTCAACCA
    GTGGCTGGTG AGGATGGCCA GGCAGCAGAT GGTCAGTTTT TATCCCTGGC TCCTGGGTCA
    ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG GAATGGGCAC AGAGCAAGGC
    TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC AGGTAATGGA GCGAAGCTTT
    CATATGCCCT CCTATGGGAC ACAGACCCTT GAAGGGGGTG TCGAGAAGCC CCATTCTCTC
    CTATCAGCTA ACCCATTATG GCAACAAAGG GCCCTGGACC CACCACACCA AATGGAGCTG
    ACTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863336.1
    CDS454..4002
    Translation

    Target ORF information:

    RefSeq Version XM_017007847.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA
    GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT
    GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT
    GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA
    GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAC
    TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC
    TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT
    GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG
    TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC
    ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG
    GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG
    TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC
    CCTCAACCTT GGTTTGTGGT TATAAAAGAA CACCAAGACC TCAAGAATGG GGGTCAACCA
    GTGGCTGGTG AGGATGGCCA GGCAGCAGAT GGTCAGTTTT TATCCCTGGC TCCTGGGTCA
    ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG GAATGGGCAC AGAGCAAGGC
    TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC AGGTAATGGA GCGAAGCTTT
    CATATGCCCT CCTATGGGAC ACAGACCCTT GAAGGGGGTG TCGAGAAGCC CCATTCTCTC
    CTATCAGCTA ACCCATTATG GCAACAAAGG GCCCTGGACC CACCACACCA AATGGAGCTG
    ACTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94953
    Clone ID Related Accession (Same CDS sequence) XM_017007848.1
    Accession Version XM_017007848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGATGTTA ATGAATGTGC TTTTTGGAAT CATGGCTGTA CTCTTGGGTG TAAAAACACC
    CCTGGATCCT ATTACTGCAC GTGCCCTGTA GGATTTGTTC TGCTTCCTGA TGGGAAACGA
    TGTCATCAAC TTGTTTCCTG TCCACGCAAT GTGTCTGAAT GCAGCCATGA CTGTGTTCTG
    ACATCAGAAG GTCCCTTATG TTTCTGTCCT GAAGGCTCAG TGCTTGAGAG AGATGGGAAA
    ACATGTAGCG GTTGTTCCTC ACCCGATAAT GGTGGATGTA GCCAGCTCTG CGTTCCTCTT
    AGCCCAGTAT CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAACT GGATGAAAAA
    AGCTGTGCAG CTTCAGGACC ACAACCATTT TTGCTGTTTG CCAATTCTCA AGATATTCGA
    CACATGCATT TTGATGGAAC AGACTATGGA ACTCTGCTCA GCCAGCAGAT GGGAATGGTT
    TATGCCCTAG ATCATGACCC TGTGGAAAAT AAGATATACT TTGCCCATAC AGCCCTGAAG
    TGGATAGAGA GAGCTAATAT GGATGGTTCC CAGCGAGAAA GGCTTATTGA GGAAGGAGTA
    GATGTGCCAG AAGGTCTTGC TGTGGACTGG ATTGGCCGTA GATTCTATTG GACAGACAGA
    GGGAAATCTC TGATTGGAAG GAGTGATTTA AATGGGAAAC GTTCCAAAAT AATCACTAAG
    GAGAACATCT CTCAACCACG AGGAATTGCT GTTCATCCAA TGGCCAAGAG ATTATTCTGG
    ACTGATACAG GGATTAATCC ACGAATTGAA AGTTCTTCCC TCCAAGGCCT TGGCCGTCTG
    GTTATAGCCA GCTCTGATCT AATCTGGCCC AGTGGAATAA CGATTGACTT CTTAACTGAC
    AAGTTGTACT GGTGCGATGC CAAGCAGTCT GTGATTGAAA TGGCCAATCT GGATGGTTCA
    AAACGCCGAA GACTTACCCA GAATGATGTA GGTCACCCAT TTGCTGTAGC AGTGTTTGAG
    GATTATGTGT GGTTCTCAGA TTGGGCTATG CCATCAGTAA TGAGAGTAAA CAAGAGGACT
    GGCAAAGATA GAGTACGTCT CCAAGGCAGC ATGCTGAAGC CCTCATCACT GGTTGTGGTT
    CATCCATTGG CAAAACCAGG AGCAGATCCC TGCTTATATC AAAACGGAGG CTGTGAACAT
    ATTTGCAAAA AGAGGCTTGG AACTGCTTGG TGTTCGTGTC GTGAAGGTTT TATGAAAGCC
    TCAGATGGGA AAACGTGTCT GGCTCTGGAT GGTCATCAGC TGTTGGCAGG TGGTGAAGTT
    GATCTAAAGA ACCAAGTAAC ACCATTGGAC ATCTTGTCCA AGACTAGAGT GTCAGAAGAT
    AACATTACAG AATCTCAACA CATGCTAGTG GCTGAAATCA TGGTGTCAGA TCAAGATGAC
    TGTGCTCCTG TGGGATGCAG CATGTATGCT CGGTGTATTT CAGAGGGAGA GGATGCCACA
    TGTCAGTGTT TGAAAGGATT TGCTGGGGAT GGAAAACTAT GTTCTGATAT AGATGAATGT
    GAGATGGGTG TCCCAGTGTG CCCCCCTGCC TCCTCCAAGT GCATCAACAC CGAAGGTGGT
    TATGTCTGCC GGTGCTCAGA AGGCTACCAA GGAGATGGGA TTCACTGTCT TGATATTGAT
    GAGTGCCAAC TGGGGGAGCA CAGCTGTGGA GAGAATGCCA GCTGCACAAA TACAGAGGGA
    GGCTATACCT GCATGTGTGC TGGACGCCTG TCTGAACCAG GACTGATTTG CCCTGACTCT
    ACTCCACCCC CTCACCTCAG GGAAGATGAC CACCACTATT CCGTAAGAAA TAGTGACTCT
    GAATGTCCCC TGTCCCACGA TGGGTACTGC CTCCATGATG GTGTGTGCAT GTATATTGAA
    GCATTGGACA AGTATGCATG CAACTGTGTT GTTGGCTACA TCGGGGAGCG ATGTCAGTAC
    CGAGACCTGA AGTGGTGGGA ACTGCGCCAC GCTGGCCACG GGCAGCAGCA GAAGGTCATC
    GTGGTGGCTG TCTGCGTGGT GGTGCTTGTC ATGCTGCTCC TCCTGAGCCT GTGGGGGGCC
    CACTACTACA GGACTCAGAA GCTGCTATCG AAAAACCCAA AGAATCCTTA TGAGGAGTCG
    AGCAGAGATG TGAGGAGTCG CAGGCCTGCT GACACTGAGG ATGGGATGTC CTCTTGCCCT
    CAACCTTGGT TTGTGGTTAT AAAAGAACAC CAAGACCTCA AGAATGGGGG TCAACCAGTG
    GCTGGTGAGG ATGGCCAGGC AGCAGATGGT CAGTTTTTAT CCCTGGCTCC TGGGTCAATG
    CAACCAACTT CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC
    TGGATTCCAG TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAATGGAGCG AAGCTTTCAT
    ATGCCCTCCT ATGGGACACA GACCCTTGAA GGGGGTGTCG AGAAGCCCCA TTCTCTCCTA
    TCAGCTAACC CATTATGGCA ACAAAGGGCC CTGGACCCAC CACACCAAAT GGAGCTGACT
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863337.1
    CDS454..3999
    Translation

    Target ORF information:

    RefSeq Version XM_017007848.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGATGTTA ATGAATGTGC TTTTTGGAAT CATGGCTGTA CTCTTGGGTG TAAAAACACC
    CCTGGATCCT ATTACTGCAC GTGCCCTGTA GGATTTGTTC TGCTTCCTGA TGGGAAACGA
    TGTCATCAAC TTGTTTCCTG TCCACGCAAT GTGTCTGAAT GCAGCCATGA CTGTGTTCTG
    ACATCAGAAG GTCCCTTATG TTTCTGTCCT GAAGGCTCAG TGCTTGAGAG AGATGGGAAA
    ACATGTAGCG GTTGTTCCTC ACCCGATAAT GGTGGATGTA GCCAGCTCTG CGTTCCTCTT
    AGCCCAGTAT CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAACT GGATGAAAAA
    AGCTGTGCAG CTTCAGGACC ACAACCATTT TTGCTGTTTG CCAATTCTCA AGATATTCGA
    CACATGCATT TTGATGGAAC AGACTATGGA ACTCTGCTCA GCCAGCAGAT GGGAATGGTT
    TATGCCCTAG ATCATGACCC TGTGGAAAAT AAGATATACT TTGCCCATAC AGCCCTGAAG
    TGGATAGAGA GAGCTAATAT GGATGGTTCC CAGCGAGAAA GGCTTATTGA GGAAGGAGTA
    GATGTGCCAG AAGGTCTTGC TGTGGACTGG ATTGGCCGTA GATTCTATTG GACAGACAGA
    GGGAAATCTC TGATTGGAAG GAGTGATTTA AATGGGAAAC GTTCCAAAAT AATCACTAAG
    GAGAACATCT CTCAACCACG AGGAATTGCT GTTCATCCAA TGGCCAAGAG ATTATTCTGG
    ACTGATACAG GGATTAATCC ACGAATTGAA AGTTCTTCCC TCCAAGGCCT TGGCCGTCTG
    GTTATAGCCA GCTCTGATCT AATCTGGCCC AGTGGAATAA CGATTGACTT CTTAACTGAC
    AAGTTGTACT GGTGCGATGC CAAGCAGTCT GTGATTGAAA TGGCCAATCT GGATGGTTCA
    AAACGCCGAA GACTTACCCA GAATGATGTA GGTCACCCAT TTGCTGTAGC AGTGTTTGAG
    GATTATGTGT GGTTCTCAGA TTGGGCTATG CCATCAGTAA TGAGAGTAAA CAAGAGGACT
    GGCAAAGATA GAGTACGTCT CCAAGGCAGC ATGCTGAAGC CCTCATCACT GGTTGTGGTT
    CATCCATTGG CAAAACCAGG AGCAGATCCC TGCTTATATC AAAACGGAGG CTGTGAACAT
    ATTTGCAAAA AGAGGCTTGG AACTGCTTGG TGTTCGTGTC GTGAAGGTTT TATGAAAGCC
    TCAGATGGGA AAACGTGTCT GGCTCTGGAT GGTCATCAGC TGTTGGCAGG TGGTGAAGTT
    GATCTAAAGA ACCAAGTAAC ACCATTGGAC ATCTTGTCCA AGACTAGAGT GTCAGAAGAT
    AACATTACAG AATCTCAACA CATGCTAGTG GCTGAAATCA TGGTGTCAGA TCAAGATGAC
    TGTGCTCCTG TGGGATGCAG CATGTATGCT CGGTGTATTT CAGAGGGAGA GGATGCCACA
    TGTCAGTGTT TGAAAGGATT TGCTGGGGAT GGAAAACTAT GTTCTGATAT AGATGAATGT
    GAGATGGGTG TCCCAGTGTG CCCCCCTGCC TCCTCCAAGT GCATCAACAC CGAAGGTGGT
    TATGTCTGCC GGTGCTCAGA AGGCTACCAA GGAGATGGGA TTCACTGTCT TGATATTGAT
    GAGTGCCAAC TGGGGGAGCA CAGCTGTGGA GAGAATGCCA GCTGCACAAA TACAGAGGGA
    GGCTATACCT GCATGTGTGC TGGACGCCTG TCTGAACCAG GACTGATTTG CCCTGACTCT
    ACTCCACCCC CTCACCTCAG GGAAGATGAC CACCACTATT CCGTAAGAAA TAGTGACTCT
    GAATGTCCCC TGTCCCACGA TGGGTACTGC CTCCATGATG GTGTGTGCAT GTATATTGAA
    GCATTGGACA AGTATGCATG CAACTGTGTT GTTGGCTACA TCGGGGAGCG ATGTCAGTAC
    CGAGACCTGA AGTGGTGGGA ACTGCGCCAC GCTGGCCACG GGCAGCAGCA GAAGGTCATC
    GTGGTGGCTG TCTGCGTGGT GGTGCTTGTC ATGCTGCTCC TCCTGAGCCT GTGGGGGGCC
    CACTACTACA GGACTCAGAA GCTGCTATCG AAAAACCCAA AGAATCCTTA TGAGGAGTCG
    AGCAGAGATG TGAGGAGTCG CAGGCCTGCT GACACTGAGG ATGGGATGTC CTCTTGCCCT
    CAACCTTGGT TTGTGGTTAT AAAAGAACAC CAAGACCTCA AGAATGGGGG TCAACCAGTG
    GCTGGTGAGG ATGGCCAGGC AGCAGATGGT CAGTTTTTAT CCCTGGCTCC TGGGTCAATG
    CAACCAACTT CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC
    TGGATTCCAG TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAATGGAGCG AAGCTTTCAT
    ATGCCCTCCT ATGGGACACA GACCCTTGAA GGGGGTGTCG AGAAGCCCCA TTCTCTCCTA
    TCAGCTAACC CATTATGGCA ACAAAGGGCC CTGGACCCAC CACACCAAAT GGAGCTGACT
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94954
    Clone ID Related Accession (Same CDS sequence) XM_017007849.1
    Accession Version XM_017007849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATATT
    GATGAGTGCC AACTGGGGGA GCACAGCTGT GGAGAGAATG CCAGCTGCAC AAATACAGAG
    GGAGGCTATA CCTGCATGTG TGCTGGACGC CTGTCTGAAC CAGGACTGAT TTGCCCTGAC
    TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC
    TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT
    GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG
    TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC
    ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG
    GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG
    TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC
    CCTCAACCTT GGTTTGTGGT TATAAAAGAA CACCAAGACC TCAAGAATGG GGGTCAACCA
    GTGGCTGGTG AGGATGGCCA GGCAGCAGAT GGTCAGTTTT TATCCCTGGC TCCTGGGTCA
    ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG GAATGGGCAC AGAGCAAGGC
    TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC AGGTAATGGA GCGAAGCTTT
    CATATGCCCT CCTATGGGAC ACAGACCCTT GAAGGGGGTG TCGAGAAGCC CCATTCTCTC
    CTATCAGCTA ACCCATTATG GCAACAAAGG GCCCTGGACC CACCACACCA AATGGAGCTG
    ACTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863338.1
    CDS454..3882
    Translation

    Target ORF information:

    RefSeq Version XM_017007849.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATATT
    GATGAGTGCC AACTGGGGGA GCACAGCTGT GGAGAGAATG CCAGCTGCAC AAATACAGAG
    GGAGGCTATA CCTGCATGTG TGCTGGACGC CTGTCTGAAC CAGGACTGAT TTGCCCTGAC
    TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC
    TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT
    GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG
    TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC
    ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG
    GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG
    TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC
    CCTCAACCTT GGTTTGTGGT TATAAAAGAA CACCAAGACC TCAAGAATGG GGGTCAACCA
    GTGGCTGGTG AGGATGGCCA GGCAGCAGAT GGTCAGTTTT TATCCCTGGC TCCTGGGTCA
    ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG GAATGGGCAC AGAGCAAGGC
    TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC AGGTAATGGA GCGAAGCTTT
    CATATGCCCT CCTATGGGAC ACAGACCCTT GAAGGGGGTG TCGAGAAGCC CCATTCTCTC
    CTATCAGCTA ACCCATTATG GCAACAAAGG GCCCTGGACC CACCACACCA AATGGAGCTG
    ACTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94955
    Clone ID Related Accession (Same CDS sequence) XM_017007850.1
    Accession Version XM_017007850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA
    GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT
    GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT
    GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA
    GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT
    ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA
    GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT
    GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT
    GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT
    ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT
    CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG
    GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG
    GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG
    GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT
    TGCCCTCAAC CTTGGGGTCA ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG
    GAATGGGCAC AGAGCAAGGC TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC
    AGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863339.1
    CDS454..3879
    Translation

    Target ORF information:

    RefSeq Version XM_017007850.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA
    GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT
    GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT
    GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA
    GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT
    ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA
    GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT
    GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT
    GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT
    ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT
    CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG
    GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG
    GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG
    GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT
    TGCCCTCAAC CTTGGGGTCA ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG
    GAATGGGCAC AGAGCAAGGC TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC
    AGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94956
    Clone ID Related Accession (Same CDS sequence) XM_017007851.1
    Accession Version XM_017007851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3183bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATATT
    GATGAGTGCC AACTGGGGGA GCACAGCTGT GGAGAGAATG CCAGCTGCAC AAATACAGAG
    GGAGGCTATA CCTGCATGTG TGCTGGACGC CTGTCTGAAC CAGGACTGAT TTGCCCTGAC
    TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC
    TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT
    GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG
    TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC
    ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG
    GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG
    TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC
    CCTCAACCTT GGGGTCAATG CAACCAACTT CATGGAGGCA GGAGCCCCAG TTATGTGGAA
    TGGGCACAGA GCAAGGCTGC TGGATTCCAG TATCCAGTGA TAAGGGCTCC TGTCCCCAGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863340.1
    CDS454..3636
    Translation

    Target ORF information:

    RefSeq Version XM_017007851.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT
    GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT
    GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT
    TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT
    AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG
    TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT
    CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA
    TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG
    AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG
    CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT
    TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG
    TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC
    AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT
    GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG
    CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA
    GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG
    TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA
    AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG
    AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT
    GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT
    GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT
    CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT
    GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT
    ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA
    ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC
    CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA
    GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA
    GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC
    ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC
    CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA
    ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT
    GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG
    TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG
    AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT
    GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT
    GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG
    AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT
    GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA
    GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATATT
    GATGAGTGCC AACTGGGGGA GCACAGCTGT GGAGAGAATG CCAGCTGCAC AAATACAGAG
    GGAGGCTATA CCTGCATGTG TGCTGGACGC CTGTCTGAAC CAGGACTGAT TTGCCCTGAC
    TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC
    TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT
    GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG
    TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC
    ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG
    GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG
    TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC
    CCTCAACCTT GGGGTCAATG CAACCAACTT CATGGAGGCA GGAGCCCCAG TTATGTGGAA
    TGGGCACAGA GCAAGGCTGC TGGATTCCAG TATCCAGTGA TAAGGGCTCC TGTCCCCAGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94957
    Clone ID Related Accession (Same CDS sequence) XM_017007852.1 , NM_001357021.2 , NM_001357021.1
    Accession Version XM_017007852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product pro-epidermal growth factor isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT
    TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC
    CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA
    CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC
    TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC
    GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT
    TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA
    CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC
    TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG
    GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT
    GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG
    GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT
    GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA
    ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA
    ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC
    TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG
    CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT
    GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG
    GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA
    GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA
    GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT
    GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT
    CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA
    TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG
    CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT
    GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC
    CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT
    GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC
    TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT
    GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC
    CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT
    GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA
    TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT
    GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT
    CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG
    TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863341.1
    CDS454..3486
    Translation

    Target ORF information:

    RefSeq Version XM_017007852.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT
    TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC
    CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA
    CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC
    TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC
    GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT
    TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA
    CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC
    TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG
    GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT
    GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG
    GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT
    GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA
    ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA
    ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC
    TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG
    CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT
    GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG
    GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA
    GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA
    GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT
    GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT
    CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA
    TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG
    CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT
    GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC
    CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT
    GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC
    TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT
    GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC
    CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT
    GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA
    TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT
    GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT
    CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG
    TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15827
    Clone ID Related Accession (Same CDS sequence) NM_001178131.3 , NM_001178131.2
    Accession Version NM_001178131.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, AK299306.1, AC004050.1 and CA437074.1. On May 31, 2019 this sequence version replaced NM_001178131.2. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. This isoform (3) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299306.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT
    TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC
    CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA
    CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC
    TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC
    GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT
    TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA
    CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC
    TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG
    GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT
    GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG
    GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT
    GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA
    ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA
    ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC
    TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG
    CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT
    GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG
    GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA
    GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA
    GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT
    GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT
    CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA
    TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG
    CTAGTGGCTG AAATCATGGT GTCAGATCAA GATGACTGTG CTCCTGTGGG ATGCAGCATG
    TATGCTCGGT GTATTTCAGA GGGAGAGGAT GCCACATGTC AGTGTTTGAA AGGATTTGCT
    GGGGATGGAA AACTATGTTC TGATATAGAT GAATGTGAGA TGGGTGTCCC AGTGTGCCCC
    CCTGCCTCCT CCAAGTGCAT CAACACCGAA GGTGGTTATG TCTGCCGGTG CTCAGAAGGC
    TACCAAGGAG ATGGGATTCA CTGTCTTGAT ATTGATGAGT GCCAACTGGG GGAGCACAGC
    TGTGGAGAGA ATGCCAGCTG CACAAATACA GAGGGAGGCT ATACCTGCAT GTGTGCTGGA
    CGCCTGTCTG AACCAGGACT GATTTGCCCT GACTCTACTC CACCCCCTCA CCTCAGGGAA
    GATGACCACC ACTATTCCGT AAGAAATAGT GACTCTGAAT GTCCCCTGTC CCACGATGGG
    TACTGCCTCC ATGATGGTGT GTGCATGTAT ATTGAAGCAT TGGACAAGTA TGCATGCAAC
    TGTGTTGTTG GCTACATCGG GGAGCGATGT CAGTACCGAG ACCTGAAGTG GTGGGAACTG
    CGCCACGCTG GCCACGGGCA GCAGCAGAAG GTCATCGTGG TGGCTGTCTG CGTGGTGGTG
    CTTGTCATGC TGCTCCTCCT GAGCCTGTGG GGGGCCCACT ACTACAGGAC TCAGAAGCTG
    CTATCGAAAA ACCCAAAGAA TCCTTATGAG GAGTCGAGCA GAGATGTGAG GAGTCGCAGG
    CCTGCTGACA CTGAGGATGG GATGTCCTCT TGCCCTCAAC CTTGGTTTGT GGTTATAAAA
    GAACACCAAG ACCTCAAGAA TGGGGGTCAA CCAGTGGCTG GTGAGGATGG CCAGGCAGCA
    GATGGGTCAA TGCAACCAAC TTCATGGAGG CAGGAGCCCC AGTTATGTGG AATGGGCACA
    GAGCAAGGCT GCTGGATTCC AGTATCCAGT GATAAGGGCT CCTGTCCCCA GGTAATGGAG
    CGAAGCTTTC ATATGCCCTC CTATGGGACA CAGACCCTTG AAGGGGGTGT CGAGAAGCCC
    CATTCTCTCC TATCAGCTAA CCCATTATGG CAACAAAGGG CCCTGGACCC ACCACACCAA
    ATGGAGCTGA CTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171602.1
    CDS454..3951
    Translation

    Target ORF information:

    RefSeq Version NM_001178131.3
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001178131.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT
    TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC
    CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA
    CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC
    TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC
    GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT
    TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA
    CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC
    TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG
    GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT
    GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG
    GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT
    GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA
    ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA
    ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC
    TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG
    CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT
    GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG
    GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA
    GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA
    GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT
    GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT
    CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA
    TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG
    CTAGTGGCTG AAATCATGGT GTCAGATCAA GATGACTGTG CTCCTGTGGG ATGCAGCATG
    TATGCTCGGT GTATTTCAGA GGGAGAGGAT GCCACATGTC AGTGTTTGAA AGGATTTGCT
    GGGGATGGAA AACTATGTTC TGATATAGAT GAATGTGAGA TGGGTGTCCC AGTGTGCCCC
    CCTGCCTCCT CCAAGTGCAT CAACACCGAA GGTGGTTATG TCTGCCGGTG CTCAGAAGGC
    TACCAAGGAG ATGGGATTCA CTGTCTTGAT ATTGATGAGT GCCAACTGGG GGAGCACAGC
    TGTGGAGAGA ATGCCAGCTG CACAAATACA GAGGGAGGCT ATACCTGCAT GTGTGCTGGA
    CGCCTGTCTG AACCAGGACT GATTTGCCCT GACTCTACTC CACCCCCTCA CCTCAGGGAA
    GATGACCACC ACTATTCCGT AAGAAATAGT GACTCTGAAT GTCCCCTGTC CCACGATGGG
    TACTGCCTCC ATGATGGTGT GTGCATGTAT ATTGAAGCAT TGGACAAGTA TGCATGCAAC
    TGTGTTGTTG GCTACATCGG GGAGCGATGT CAGTACCGAG ACCTGAAGTG GTGGGAACTG
    CGCCACGCTG GCCACGGGCA GCAGCAGAAG GTCATCGTGG TGGCTGTCTG CGTGGTGGTG
    CTTGTCATGC TGCTCCTCCT GAGCCTGTGG GGGGCCCACT ACTACAGGAC TCAGAAGCTG
    CTATCGAAAA ACCCAAAGAA TCCTTATGAG GAGTCGAGCA GAGATGTGAG GAGTCGCAGG
    CCTGCTGACA CTGAGGATGG GATGTCCTCT TGCCCTCAAC CTTGGTTTGT GGTTATAAAA
    GAACACCAAG ACCTCAAGAA TGGGGGTCAA CCAGTGGCTG GTGAGGATGG CCAGGCAGCA
    GATGGGTCAA TGCAACCAAC TTCATGGAGG CAGGAGCCCC AGTTATGTGG AATGGGCACA
    GAGCAAGGCT GCTGGATTCC AGTATCCAGT GATAAGGGCT CCTGTCCCCA GGTAATGGAG
    CGAAGCTTTC ATATGCCCTC CTATGGGACA CAGACCCTTG AAGGGGGTGT CGAGAAGCCC
    CATTCTCTCC TATCAGCTAA CCCATTATGG CAACAAAGGG CCCTGGACCC ACCACACCAA
    ATGGAGCTGA CTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15827
    Clone ID Related Accession (Same CDS sequence) NM_001178131.3 , NM_001178131.2
    Accession Version NM_001178131.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299306.1, AC005509.1, AC004050.1 and CA437074.1. On Jan 8, 2016 this sequence version replaced NM_001178131.1. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. This isoform (3) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299306.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT
    TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC
    CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA
    CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC
    TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC
    GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT
    TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA
    CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC
    TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG
    GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT
    GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG
    GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT
    GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA
    ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA
    ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC
    TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG
    CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT
    GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG
    GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA
    GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA
    GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT
    GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT
    CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA
    TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG
    CTAGTGGCTG AAATCATGGT GTCAGATCAA GATGACTGTG CTCCTGTGGG ATGCAGCATG
    TATGCTCGGT GTATTTCAGA GGGAGAGGAT GCCACATGTC AGTGTTTGAA AGGATTTGCT
    GGGGATGGAA AACTATGTTC TGATATAGAT GAATGTGAGA TGGGTGTCCC AGTGTGCCCC
    CCTGCCTCCT CCAAGTGCAT CAACACCGAA GGTGGTTATG TCTGCCGGTG CTCAGAAGGC
    TACCAAGGAG ATGGGATTCA CTGTCTTGAT ATTGATGAGT GCCAACTGGG GGAGCACAGC
    TGTGGAGAGA ATGCCAGCTG CACAAATACA GAGGGAGGCT ATACCTGCAT GTGTGCTGGA
    CGCCTGTCTG AACCAGGACT GATTTGCCCT GACTCTACTC CACCCCCTCA CCTCAGGGAA
    GATGACCACC ACTATTCCGT AAGAAATAGT GACTCTGAAT GTCCCCTGTC CCACGATGGG
    TACTGCCTCC ATGATGGTGT GTGCATGTAT ATTGAAGCAT TGGACAAGTA TGCATGCAAC
    TGTGTTGTTG GCTACATCGG GGAGCGATGT CAGTACCGAG ACCTGAAGTG GTGGGAACTG
    CGCCACGCTG GCCACGGGCA GCAGCAGAAG GTCATCGTGG TGGCTGTCTG CGTGGTGGTG
    CTTGTCATGC TGCTCCTCCT GAGCCTGTGG GGGGCCCACT ACTACAGGAC TCAGAAGCTG
    CTATCGAAAA ACCCAAAGAA TCCTTATGAG GAGTCGAGCA GAGATGTGAG GAGTCGCAGG
    CCTGCTGACA CTGAGGATGG GATGTCCTCT TGCCCTCAAC CTTGGTTTGT GGTTATAAAA
    GAACACCAAG ACCTCAAGAA TGGGGGTCAA CCAGTGGCTG GTGAGGATGG CCAGGCAGCA
    GATGGGTCAA TGCAACCAAC TTCATGGAGG CAGGAGCCCC AGTTATGTGG AATGGGCACA
    GAGCAAGGCT GCTGGATTCC AGTATCCAGT GATAAGGGCT CCTGTCCCCA GGTAATGGAG
    CGAAGCTTTC ATATGCCCTC CTATGGGACA CAGACCCTTG AAGGGGGTGT CGAGAAGCCC
    CATTCTCTCC TATCAGCTAA CCCATTATGG CAACAAAGGG CCCTGGACCC ACCACACCAA
    ATGGAGCTGA CTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171602.1
    CDS453..3950
    Translation

    Target ORF information:

    RefSeq Version NM_001178131.2
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001178131.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT
    TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC
    CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA
    CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC
    TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC
    GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT
    TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA
    CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC
    TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG
    GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT
    GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG
    GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT
    GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA
    ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA
    ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC
    TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG
    CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT
    GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG
    GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA
    GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA
    GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT
    GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT
    CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA
    TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG
    CTAGTGGCTG AAATCATGGT GTCAGATCAA GATGACTGTG CTCCTGTGGG ATGCAGCATG
    TATGCTCGGT GTATTTCAGA GGGAGAGGAT GCCACATGTC AGTGTTTGAA AGGATTTGCT
    GGGGATGGAA AACTATGTTC TGATATAGAT GAATGTGAGA TGGGTGTCCC AGTGTGCCCC
    CCTGCCTCCT CCAAGTGCAT CAACACCGAA GGTGGTTATG TCTGCCGGTG CTCAGAAGGC
    TACCAAGGAG ATGGGATTCA CTGTCTTGAT ATTGATGAGT GCCAACTGGG GGAGCACAGC
    TGTGGAGAGA ATGCCAGCTG CACAAATACA GAGGGAGGCT ATACCTGCAT GTGTGCTGGA
    CGCCTGTCTG AACCAGGACT GATTTGCCCT GACTCTACTC CACCCCCTCA CCTCAGGGAA
    GATGACCACC ACTATTCCGT AAGAAATAGT GACTCTGAAT GTCCCCTGTC CCACGATGGG
    TACTGCCTCC ATGATGGTGT GTGCATGTAT ATTGAAGCAT TGGACAAGTA TGCATGCAAC
    TGTGTTGTTG GCTACATCGG GGAGCGATGT CAGTACCGAG ACCTGAAGTG GTGGGAACTG
    CGCCACGCTG GCCACGGGCA GCAGCAGAAG GTCATCGTGG TGGCTGTCTG CGTGGTGGTG
    CTTGTCATGC TGCTCCTCCT GAGCCTGTGG GGGGCCCACT ACTACAGGAC TCAGAAGCTG
    CTATCGAAAA ACCCAAAGAA TCCTTATGAG GAGTCGAGCA GAGATGTGAG GAGTCGCAGG
    CCTGCTGACA CTGAGGATGG GATGTCCTCT TGCCCTCAAC CTTGGTTTGT GGTTATAAAA
    GAACACCAAG ACCTCAAGAA TGGGGGTCAA CCAGTGGCTG GTGAGGATGG CCAGGCAGCA
    GATGGGTCAA TGCAACCAAC TTCATGGAGG CAGGAGCCCC AGTTATGTGG AATGGGCACA
    GAGCAAGGCT GCTGGATTCC AGTATCCAGT GATAAGGGCT CCTGTCCCCA GGTAATGGAG
    CGAAGCTTTC ATATGCCCTC CTATGGGACA CAGACCCTTG AAGGGGGTGT CGAGAAGCCC
    CATTCTCTCC TATCAGCTAA CCCATTATGG CAACAAAGGG CCCTGGACCC ACCACACCAA
    ATGGAGCTGA CTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16028
    Clone ID Related Accession (Same CDS sequence) NM_001178130.2 , NM_001178130.3
    Accession Version NM_001178130.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, BC143357.1, AC004050.1 and CA437074.1. On Jan 8, 2016 this sequence version replaced NM_001178130.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143357.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG
    AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG
    TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT
    GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC
    ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC
    TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA
    TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC
    TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA
    TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA
    CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA
    ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC
    CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT
    ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT
    GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA
    AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA
    CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT
    CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT
    CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT
    GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC
    CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA
    GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT
    CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA
    GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT
    GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT
    CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA
    ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA
    CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC
    AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA
    TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG
    TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA
    GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGACTCTA CTCCACCCCC TCACCTCAGG
    GAAGATGACC ACCACTATTC CGTAAGAAAT AGTGACTCTG AATGTCCCCT GTCCCACGAT
    GGGTACTGCC TCCATGATGG TGTGTGCATG TATATTGAAG CATTGGACAA GTATGCATGC
    AACTGTGTTG TTGGCTACAT CGGGGAGCGA TGTCAGTACC GAGACCTGAA GTGGTGGGAA
    CTGCGCCACG CTGGCCACGG GCAGCAGCAG AAGGTCATCG TGGTGGCTGT CTGCGTGGTG
    GTGCTTGTCA TGCTGCTCCT CCTGAGCCTG TGGGGGGCCC ACTACTACAG GACTCAGAAG
    CTGCTATCGA AAAACCCAAA GAATCCTTAT GAGGAGTCGA GCAGAGATGT GAGGAGTCGC
    AGGCCTGCTG ACACTGAGGA TGGGATGTCC TCTTGCCCTC AACCTTGGTT TGTGGTTATA
    AAAGAACACC AAGACCTCAA GAATGGGGGT CAACCAGTGG CTGGTGAGGA TGGCCAGGCA
    GCAGATGGGT CAATGCAACC AACTTCATGG AGGCAGGAGC CCCAGTTATG TGGAATGGGC
    ACAGAGCAAG GCTGCTGGAT TCCAGTATCC AGTGATAAGG GCTCCTGTCC CCAGGTAATG
    GAGCGAAGCT TTCATATGCC CTCCTATGGG ACACAGACCC TTGAAGGGGG TGTCGAGAAG
    CCCCATTCTC TCCTATCAGC TAACCCATTA TGGCAACAAA GGGCCCTGGA CCCACCACAC
    CAAATGGAGC TGACTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171601.1
    CDS453..3953
    Translation

    Target ORF information:

    RefSeq Version NM_001178130.2
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001178130.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG
    AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG
    TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT
    GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC
    ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC
    TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA
    TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC
    TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA
    TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA
    CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA
    ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC
    CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT
    ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT
    GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA
    AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA
    CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT
    CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT
    CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT
    GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC
    CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA
    GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT
    CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA
    GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT
    GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT
    CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA
    ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA
    CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC
    AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA
    TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG
    TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA
    GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGACTCTA CTCCACCCCC TCACCTCAGG
    GAAGATGACC ACCACTATTC CGTAAGAAAT AGTGACTCTG AATGTCCCCT GTCCCACGAT
    GGGTACTGCC TCCATGATGG TGTGTGCATG TATATTGAAG CATTGGACAA GTATGCATGC
    AACTGTGTTG TTGGCTACAT CGGGGAGCGA TGTCAGTACC GAGACCTGAA GTGGTGGGAA
    CTGCGCCACG CTGGCCACGG GCAGCAGCAG AAGGTCATCG TGGTGGCTGT CTGCGTGGTG
    GTGCTTGTCA TGCTGCTCCT CCTGAGCCTG TGGGGGGCCC ACTACTACAG GACTCAGAAG
    CTGCTATCGA AAAACCCAAA GAATCCTTAT GAGGAGTCGA GCAGAGATGT GAGGAGTCGC
    AGGCCTGCTG ACACTGAGGA TGGGATGTCC TCTTGCCCTC AACCTTGGTT TGTGGTTATA
    AAAGAACACC AAGACCTCAA GAATGGGGGT CAACCAGTGG CTGGTGAGGA TGGCCAGGCA
    GCAGATGGGT CAATGCAACC AACTTCATGG AGGCAGGAGC CCCAGTTATG TGGAATGGGC
    ACAGAGCAAG GCTGCTGGAT TCCAGTATCC AGTGATAAGG GCTCCTGTCC CCAGGTAATG
    GAGCGAAGCT TTCATATGCC CTCCTATGGG ACACAGACCC TTGAAGGGGG TGTCGAGAAG
    CCCCATTCTC TCCTATCAGC TAACCCATTA TGGCAACAAA GGGCCCTGGA CCCACCACAC
    CAAATGGAGC TGACTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16028
    Clone ID Related Accession (Same CDS sequence) NM_001178130.2 , NM_001178130.3
    Accession Version NM_001178130.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, BC143357.1, AC004050.1 and CA437074.1. On May 31, 2019 this sequence version replaced NM_001178130.2. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143357.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG
    AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG
    TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT
    GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC
    ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC
    TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA
    TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC
    TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA
    TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA
    CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA
    ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC
    CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT
    ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT
    GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA
    AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA
    CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT
    CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT
    CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT
    GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC
    CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA
    GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT
    CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA
    GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT
    GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT
    CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA
    ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA
    CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC
    AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA
    TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG
    TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA
    GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGACTCTA CTCCACCCCC TCACCTCAGG
    GAAGATGACC ACCACTATTC CGTAAGAAAT AGTGACTCTG AATGTCCCCT GTCCCACGAT
    GGGTACTGCC TCCATGATGG TGTGTGCATG TATATTGAAG CATTGGACAA GTATGCATGC
    AACTGTGTTG TTGGCTACAT CGGGGAGCGA TGTCAGTACC GAGACCTGAA GTGGTGGGAA
    CTGCGCCACG CTGGCCACGG GCAGCAGCAG AAGGTCATCG TGGTGGCTGT CTGCGTGGTG
    GTGCTTGTCA TGCTGCTCCT CCTGAGCCTG TGGGGGGCCC ACTACTACAG GACTCAGAAG
    CTGCTATCGA AAAACCCAAA GAATCCTTAT GAGGAGTCGA GCAGAGATGT GAGGAGTCGC
    AGGCCTGCTG ACACTGAGGA TGGGATGTCC TCTTGCCCTC AACCTTGGTT TGTGGTTATA
    AAAGAACACC AAGACCTCAA GAATGGGGGT CAACCAGTGG CTGGTGAGGA TGGCCAGGCA
    GCAGATGGGT CAATGCAACC AACTTCATGG AGGCAGGAGC CCCAGTTATG TGGAATGGGC
    ACAGAGCAAG GCTGCTGGAT TCCAGTATCC AGTGATAAGG GCTCCTGTCC CCAGGTAATG
    GAGCGAAGCT TTCATATGCC CTCCTATGGG ACACAGACCC TTGAAGGGGG TGTCGAGAAG
    CCCCATTCTC TCCTATCAGC TAACCCATTA TGGCAACAAA GGGCCCTGGA CCCACCACAC
    CAAATGGAGC TGACTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171601.1
    CDS454..3954
    Translation

    Target ORF information:

    RefSeq Version NM_001178130.3
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001178130.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG
    AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG
    TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT
    GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC
    ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC
    TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA
    TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC
    TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA
    TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA
    CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA
    ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC
    CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT
    ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT
    GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA
    AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA
    CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT
    CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT
    CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT
    GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC
    CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA
    GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT
    CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA
    GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT
    GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT
    CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA
    ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA
    CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC
    AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA
    TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG
    TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA
    GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGACTCTA CTCCACCCCC TCACCTCAGG
    GAAGATGACC ACCACTATTC CGTAAGAAAT AGTGACTCTG AATGTCCCCT GTCCCACGAT
    GGGTACTGCC TCCATGATGG TGTGTGCATG TATATTGAAG CATTGGACAA GTATGCATGC
    AACTGTGTTG TTGGCTACAT CGGGGAGCGA TGTCAGTACC GAGACCTGAA GTGGTGGGAA
    CTGCGCCACG CTGGCCACGG GCAGCAGCAG AAGGTCATCG TGGTGGCTGT CTGCGTGGTG
    GTGCTTGTCA TGCTGCTCCT CCTGAGCCTG TGGGGGGCCC ACTACTACAG GACTCAGAAG
    CTGCTATCGA AAAACCCAAA GAATCCTTAT GAGGAGTCGA GCAGAGATGT GAGGAGTCGC
    AGGCCTGCTG ACACTGAGGA TGGGATGTCC TCTTGCCCTC AACCTTGGTT TGTGGTTATA
    AAAGAACACC AAGACCTCAA GAATGGGGGT CAACCAGTGG CTGGTGAGGA TGGCCAGGCA
    GCAGATGGGT CAATGCAACC AACTTCATGG AGGCAGGAGC CCCAGTTATG TGGAATGGGC
    ACAGAGCAAG GCTGCTGGAT TCCAGTATCC AGTGATAAGG GCTCCTGTCC CCAGGTAATG
    GAGCGAAGCT TTCATATGCC CTCCTATGGG ACACAGACCC TTGAAGGGGG TGTCGAGAAG
    CCCCATTCTC TCCTATCAGC TAACCCATTA TGGCAACAAA GGGCCCTGGA CCCACCACAC
    CAAATGGAGC TGACTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38745
    Clone ID Related Accession (Same CDS sequence) XM_005262796.2
    Accession Version XM_005262796.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005262796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG
    AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG
    TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT
    GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC
    ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC
    TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA
    TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC
    TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA
    TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA
    CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA
    ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC
    CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT
    ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT
    GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA
    AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA
    CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT
    CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT
    CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT
    GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC
    CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA
    GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT
    CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA
    GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT
    GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT
    CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA
    ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA
    CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC
    AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA
    TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG
    TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA
    GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG
    CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT
    GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC
    AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC
    GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA
    TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG
    GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG
    GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG
    AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT
    CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT
    ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG
    GCAGCAGATG GTCAGTTTTT ATCCCTGGCT CCTGGGTCAA TGCAACCAAC TTCATGGAGG
    CAGGAGCCCC AGTTATGTGG AATGGGCACA GAGCAAGGCT GCTGGATTCC AGTATCCAGT
    GATAAGGGCT CCTGTCCCCA GGTAATGGAG CGAAGCTTTC ATATGCCCTC CTATGGGACA
    CAGACCCTTG AAGGGGGTGT CGAGAAGCCC CATTCTCTCC TATCAGCTAA CCCATTATGG
    CAACAAAGGG CCCTGGACCC ACCACACCAA ATGGAGCTGA CTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005262853.1
    CDS454..4101
    Translation

    Target ORF information:

    RefSeq Version XM_005262796.2
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005262796.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
    TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT
    TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC
    ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT
    TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA
    GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA
    ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT
    ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT
    GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA
    AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG
    AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC
    AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT
    AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC
    TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG
    GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA
    CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG
    AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG
    TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT
    GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC
    ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC
    TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA
    TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC
    TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA
    TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA
    CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA
    ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC
    CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT
    ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT
    GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA
    AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA
    CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT
    CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT
    CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT
    GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC
    CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA
    GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT
    CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA
    GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT
    GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT
    CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA
    ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA
    CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC
    AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA
    TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG
    TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA
    GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG
    CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT
    GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC
    AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC
    GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA
    TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG
    GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG
    GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG
    AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT
    CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT
    ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG
    GCAGCAGATG GTCAGTTTTT ATCCCTGGCT CCTGGGTCAA TGCAACCAAC TTCATGGAGG
    CAGGAGCCCC AGTTATGTGG AATGGGCACA GAGCAAGGCT GCTGGATTCC AGTATCCAGT
    GATAAGGGCT CCTGTCCCCA GGTAATGGAG CGAAGCTTTC ATATGCCCTC CTATGGGACA
    CAGACCCTTG AAGGGGGTGT CGAGAAGCCC CATTCTCTCC TATCAGCTAA CCCATTATGG
    CAACAAAGGG CCCTGGACCC ACCACACCAA ATGGAGCTGA CTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57444
    Clone ID Related Accession (Same CDS sequence) XM_011531707.1
    Accession Version XM_011531707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pro-epidermal growth factor isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAAGA TTTCATGTCC TGCACCCTTC TTAATTTTCT CCCATGGAAA TAGTATCTTT 
    AGGATTGACA CAGAAGGAAC CAATTATGAG CAATTGGTGG TGGATGCTGG TGTCTCAGTG
    ATCATGGATT TTCATTATAA TGAGAAAAGA ATCTATTGGG TGGATTTAGA AAGACAACTT
    TTGCAAAGAG TTTTTCTGAA TGGGTCAAGG CAAGAGAGAG TATGTAATAT AGAGAAAAAT
    GTTTCTGGAA TGGCAATAAA TTGGATAAAT GAAGAAGTTA TTTGGTCAAA TCAACAGGAA
    GGAATCATTA CAGTAACAGA TATGAAAGGA AATAATTCCC ACATTCTTTT AAGTGCTTTA
    AAATATCCTG CAAATGTAGC AGTTGATCCA GTAGAAAGGT TTATATTTTG GTCTTCAGAG
    GTGGCTGGAA GCCTTTATAG AGCAGATCTC GATGGTGTGG GAGTGAAGGC TCTGTTGGAG
    ACATCAGAGA AAATAACAGC TGTGTCATTG GATGTGCTTG ATAAGCGGCT GTTTTGGATT
    CAGTACAACA GAGAAGGAAG CAATTCTCTT ATTTGCTCCT GTGATTATGA TGGAGGTTCT
    GTCCACATTA GTAAACATCC AACACAGCAT AATTTGTTTG CAATGTCCCT TTTTGGTGAC
    CGTATCTTCT ATTCAACATG GAAAATGAAG ACAATTTGGA TAGCCAACAA ACACACTGGA
    AAGGACATGG TTAGAATTAA CCTCCATTCA TCATTTGTAC CACTTGGTGA ACTGAAAGTA
    GTGCATCCAC TTGCACAACC CAAGGCAGAA GATGACACTT GGGAGCCTGA GCAGAAACTT
    TGCAAATTGA GGAAAGGAAA CTGCAGCAGC ACTGTGTGTG GGCAAGACCT CCAGTCACAC
    TTGTGCATGT GTGCAGAGGG ATACGCCCTA AGTCGAGACC GGAAGTACTG TGAAGATGTT
    AATGAATGTG CTTTTTGGAA TCATGGCTGT ACTCTTGGGT GTAAAAACAC CCCTGGATCC
    TATTACTGCA CGTGCCCTGT AGGATTTGTT CTGCTTCCTG ATGGGAAACG ATGTCATCAA
    CTTGTTTCCT GTCCACGCAA TGTGTCTGAA TGCAGCCATG ACTGTGTTCT GACATCAGAA
    GGTCCCTTAT GTTTCTGTCC TGAAGGCTCA GTGCTTGAGA GAGATGGGAA AACATGTAGC
    GGTTGTTCCT CACCCGATAA TGGTGGATGT AGCCAGCTCT GCGTTCCTCT TAGCCCAGTA
    TCCTGGGAAT GTGATTGCTT TCCTGGGTAT GACCTACAAC TGGATGAAAA AAGCTGTGCA
    GCTTCAGGAC CACAACCATT TTTGCTGTTT GCCAATTCTC AAGATATTCG ACACATGCAT
    TTTGATGGAA CAGACTATGG AACTCTGCTC AGCCAGCAGA TGGGAATGGT TTATGCCCTA
    GATCATGACC CTGTGGAAAA TAAGATATAC TTTGCCCATA CAGCCCTGAA GTGGATAGAG
    AGAGCTAATA TGGATGGTTC CCAGCGAGAA AGGCTTATTG AGGAAGGAGT AGATGTGCCA
    GAAGGTCTTG CTGTGGACTG GATTGGCCGT AGATTCTATT GGACAGACAG AGGGAAATCT
    CTGATTGGAA GGAGTGATTT AAATGGGAAA CGTTCCAAAA TAATCACTAA GGAGAACATC
    TCTCAACCAC GAGGAATTGC TGTTCATCCA ATGGCCAAGA GATTATTCTG GACTGATACA
    GGGATTAATC CACGAATTGA AAGTTCTTCC CTCCAAGGCC TTGGCCGTCT GGTTATAGCC
    AGCTCTGATC TAATCTGGCC CAGTGGAATA ACGATTGACT TCTTAACTGA CAAGTTGTAC
    TGGTGCGATG CCAAGCAGTC TGTGATTGAA ATGGCCAATC TGGATGGTTC AAAACGCCGA
    AGACTTACCC AGAATGATGT AGGTCACCCA TTTGCTGTAG CAGTGTTTGA GGATTATGTG
    TGGTTCTCAG ATTGGGCTAT GCCATCAGTA ATGAGAGTAA ACAAGAGGAC TGGCAAAGAT
    AGAGTACGTC TCCAAGGCAG CATGCTGAAG CCCTCATCAC TGGTTGTGGT TCATCCATTG
    GCAAAACCAG GAGCAGATCC CTGCTTATAT CAAAACGGAG GCTGTGAACA TATTTGCAAA
    AAGAGGCTTG GAACTGCTTG GTGTTCGTGT CGTGAAGGTT TTATGAAAGC CTCAGATGGG
    AAAACGTGTC TGGCTCTGGA TGGTCATCAG CTGTTGGCAG GTGGTGAAGT TGATCTAAAG
    AACCAAGTAA CACCATTGGA CATCTTGTCC AAGACTAGAG TGTCAGAAGA TAACATTACA
    GAATCTCAAC ACATGCTAGT GGCTGAAATC ATGGTGTCAG ATCAAGATGA CTGTGCTCCT
    GTGGGATGCA GCATGTATGC TCGGTGTATT TCAGAGGGAG AGGATGCCAC ATGTCAGTGT
    TTGAAAGGAT TTGCTGGGGA TGGAAAACTA TGTTCTGATA TAGATGAATG TGAGATGGGT
    GTCCCAGTGT GCCCCCCTGC CTCCTCCAAG TGCATCAACA CCGAAGGTGG TTATGTCTGC
    CGGTGCTCAG AAGGCTACCA AGGAGATGGG ATTCACTGTC TTGATATTGA TGAGTGCCAA
    CTGGGGGAGC ACAGCTGTGG AGAGAATGCC AGCTGCACAA ATACAGAGGG AGGCTATACC
    TGCATGTGTG CTGGACGCCT GTCTGAACCA GGACTGATTT GCCCTGACTC TACTCCACCC
    CCTCACCTCA GGGAAGATGA CCACCACTAT TCCGTAAGAA ATAGTGACTC TGAATGTCCC
    CTGTCCCACG ATGGGTACTG CCTCCATGAT GGTGTGTGCA TGTATATTGA AGCATTGGAC
    AAGTATGCAT GCAACTGTGT TGTTGGCTAC ATCGGGGAGC GATGTCAGTA CCGAGACCTG
    AAGTGGTGGG AACTGCGCCA CGCTGGCCAC GGGCAGCAGC AGAAGGTCAT CGTGGTGGCT
    GTCTGCGTGG TGGTGCTTGT CATGCTGCTC CTCCTGAGCC TGTGGGGGGC CCACTACTAC
    AGGACTCAGA AGCTGCTATC GAAAAACCCA AAGAATCCTT ATGAGGAGTC GAGCAGAGAT
    GTGAGGAGTC GCAGGCCTGC TGACACTGAG GATGGGATGT CCTCTTGCCC TCAACCTTGG
    TTTGTGGTTA TAAAAGAACA CCAAGACCTC AAGAATGGGG GTCAACCAGT GGCTGGTGAG
    GATGGCCAGG CAGCAGATGG TCAGTTTTTA TCCCTGGCTC CTGGGTCAAT GCAACCAACT
    TCATGGAGGC AGGAGCCCCA GTTATGTGGA ATGGGCACAG AGCAAGGCTG CTGGATTCCA
    GTATCCAGTG ATAAGGGCTC CTGTCCCCAG GTAATGGAGC GAAGCTTTCA TATGCCCTCC
    TATGGGACAC AGACCCTTGA AGGGGGTGTC GAGAAGCCCC ATTCTCTCCT ATCAGCTAAC
    CCATTATGGC AACAAAGGGC CCTGGACCCA CCACACCAAA TGGAGCTGAC TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530009.1
    CDS214..3750
    Translation

    Target ORF information:

    RefSeq Version XM_011531707.1
    Organism Homo sapiens(human)
    Definition Homo sapiens epidermal growth factor (EGF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011531707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAAAGA TTTCATGTCC TGCACCCTTC TTAATTTTCT CCCATGGAAA TAGTATCTTT 
    AGGATTGACA CAGAAGGAAC CAATTATGAG CAATTGGTGG TGGATGCTGG TGTCTCAGTG
    ATCATGGATT TTCATTATAA TGAGAAAAGA ATCTATTGGG TGGATTTAGA AAGACAACTT
    TTGCAAAGAG TTTTTCTGAA TGGGTCAAGG CAAGAGAGAG TATGTAATAT AGAGAAAAAT
    GTTTCTGGAA TGGCAATAAA TTGGATAAAT GAAGAAGTTA TTTGGTCAAA TCAACAGGAA
    GGAATCATTA CAGTAACAGA TATGAAAGGA AATAATTCCC ACATTCTTTT AAGTGCTTTA
    AAATATCCTG CAAATGTAGC AGTTGATCCA GTAGAAAGGT TTATATTTTG GTCTTCAGAG
    GTGGCTGGAA GCCTTTATAG AGCAGATCTC GATGGTGTGG GAGTGAAGGC TCTGTTGGAG
    ACATCAGAGA AAATAACAGC TGTGTCATTG GATGTGCTTG ATAAGCGGCT GTTTTGGATT
    CAGTACAACA GAGAAGGAAG CAATTCTCTT ATTTGCTCCT GTGATTATGA TGGAGGTTCT
    GTCCACATTA GTAAACATCC AACACAGCAT AATTTGTTTG CAATGTCCCT TTTTGGTGAC
    CGTATCTTCT ATTCAACATG GAAAATGAAG ACAATTTGGA TAGCCAACAA ACACACTGGA
    AAGGACATGG TTAGAATTAA CCTCCATTCA TCATTTGTAC CACTTGGTGA ACTGAAAGTA
    GTGCATCCAC TTGCACAACC CAAGGCAGAA GATGACACTT GGGAGCCTGA GCAGAAACTT
    TGCAAATTGA GGAAAGGAAA CTGCAGCAGC ACTGTGTGTG GGCAAGACCT CCAGTCACAC
    TTGTGCATGT GTGCAGAGGG ATACGCCCTA AGTCGAGACC GGAAGTACTG TGAAGATGTT
    AATGAATGTG CTTTTTGGAA TCATGGCTGT ACTCTTGGGT GTAAAAACAC CCCTGGATCC
    TATTACTGCA CGTGCCCTGT AGGATTTGTT CTGCTTCCTG ATGGGAAACG ATGTCATCAA
    CTTGTTTCCT GTCCACGCAA TGTGTCTGAA TGCAGCCATG ACTGTGTTCT GACATCAGAA
    GGTCCCTTAT GTTTCTGTCC TGAAGGCTCA GTGCTTGAGA GAGATGGGAA AACATGTAGC
    GGTTGTTCCT CACCCGATAA TGGTGGATGT AGCCAGCTCT GCGTTCCTCT TAGCCCAGTA
    TCCTGGGAAT GTGATTGCTT TCCTGGGTAT GACCTACAAC TGGATGAAAA AAGCTGTGCA
    GCTTCAGGAC CACAACCATT TTTGCTGTTT GCCAATTCTC AAGATATTCG ACACATGCAT
    TTTGATGGAA CAGACTATGG AACTCTGCTC AGCCAGCAGA TGGGAATGGT TTATGCCCTA
    GATCATGACC CTGTGGAAAA TAAGATATAC TTTGCCCATA CAGCCCTGAA GTGGATAGAG
    AGAGCTAATA TGGATGGTTC CCAGCGAGAA AGGCTTATTG AGGAAGGAGT AGATGTGCCA
    GAAGGTCTTG CTGTGGACTG GATTGGCCGT AGATTCTATT GGACAGACAG AGGGAAATCT
    CTGATTGGAA GGAGTGATTT AAATGGGAAA CGTTCCAAAA TAATCACTAA GGAGAACATC
    TCTCAACCAC GAGGAATTGC TGTTCATCCA ATGGCCAAGA GATTATTCTG GACTGATACA
    GGGATTAATC CACGAATTGA AAGTTCTTCC CTCCAAGGCC TTGGCCGTCT GGTTATAGCC
    AGCTCTGATC TAATCTGGCC CAGTGGAATA ACGATTGACT TCTTAACTGA CAAGTTGTAC
    TGGTGCGATG CCAAGCAGTC TGTGATTGAA ATGGCCAATC TGGATGGTTC AAAACGCCGA
    AGACTTACCC AGAATGATGT AGGTCACCCA TTTGCTGTAG CAGTGTTTGA GGATTATGTG
    TGGTTCTCAG ATTGGGCTAT GCCATCAGTA ATGAGAGTAA ACAAGAGGAC TGGCAAAGAT
    AGAGTACGTC TCCAAGGCAG CATGCTGAAG CCCTCATCAC TGGTTGTGGT TCATCCATTG
    GCAAAACCAG GAGCAGATCC CTGCTTATAT CAAAACGGAG GCTGTGAACA TATTTGCAAA
    AAGAGGCTTG GAACTGCTTG GTGTTCGTGT CGTGAAGGTT TTATGAAAGC CTCAGATGGG
    AAAACGTGTC TGGCTCTGGA TGGTCATCAG CTGTTGGCAG GTGGTGAAGT TGATCTAAAG
    AACCAAGTAA CACCATTGGA CATCTTGTCC AAGACTAGAG TGTCAGAAGA TAACATTACA
    GAATCTCAAC ACATGCTAGT GGCTGAAATC ATGGTGTCAG ATCAAGATGA CTGTGCTCCT
    GTGGGATGCA GCATGTATGC TCGGTGTATT TCAGAGGGAG AGGATGCCAC ATGTCAGTGT
    TTGAAAGGAT TTGCTGGGGA TGGAAAACTA TGTTCTGATA TAGATGAATG TGAGATGGGT
    GTCCCAGTGT GCCCCCCTGC CTCCTCCAAG TGCATCAACA CCGAAGGTGG TTATGTCTGC
    CGGTGCTCAG AAGGCTACCA AGGAGATGGG ATTCACTGTC TTGATATTGA TGAGTGCCAA
    CTGGGGGAGC ACAGCTGTGG AGAGAATGCC AGCTGCACAA ATACAGAGGG AGGCTATACC
    TGCATGTGTG CTGGACGCCT GTCTGAACCA GGACTGATTT GCCCTGACTC TACTCCACCC
    CCTCACCTCA GGGAAGATGA CCACCACTAT TCCGTAAGAA ATAGTGACTC TGAATGTCCC
    CTGTCCCACG ATGGGTACTG CCTCCATGAT GGTGTGTGCA TGTATATTGA AGCATTGGAC
    AAGTATGCAT GCAACTGTGT TGTTGGCTAC ATCGGGGAGC GATGTCAGTA CCGAGACCTG
    AAGTGGTGGG AACTGCGCCA CGCTGGCCAC GGGCAGCAGC AGAAGGTCAT CGTGGTGGCT
    GTCTGCGTGG TGGTGCTTGT CATGCTGCTC CTCCTGAGCC TGTGGGGGGC CCACTACTAC
    AGGACTCAGA AGCTGCTATC GAAAAACCCA AAGAATCCTT ATGAGGAGTC GAGCAGAGAT
    GTGAGGAGTC GCAGGCCTGC TGACACTGAG GATGGGATGT CCTCTTGCCC TCAACCTTGG
    TTTGTGGTTA TAAAAGAACA CCAAGACCTC AAGAATGGGG GTCAACCAGT GGCTGGTGAG
    GATGGCCAGG CAGCAGATGG TCAGTTTTTA TCCCTGGCTC CTGGGTCAAT GCAACCAACT
    TCATGGAGGC AGGAGCCCCA GTTATGTGGA ATGGGCACAG AGCAAGGCTG CTGGATTCCA
    GTATCCAGTG ATAAGGGCTC CTGTCCCCAG GTAATGGAGC GAAGCTTTCA TATGCCCTCC
    TATGGGACAC AGACCCTTGA AGGGGGTGTC GAGAAGCCCC ATTCTCTCCT ATCAGCTAAC
    CCATTATGGC AACAAAGGGC CCTGGACCCA CCACACCAAA TGGAGCTGAC TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.