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EN1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol EN1
Entrez Gene ID 2019
Full Name engrailed homeobox 1
General protein information
Preferred Names

engrailed homeobox 1

Names

homeobox protein engrailed-1
engrailed homolog 1
homeobox protein en-1
hu-En-1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

2

2q14.2

Summary Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008].
Disorder MIM:

131290


mRNA and Protein(s)

mRNA Protein Name
NM_001426.3 NP_001417.3 homeobox protein engrailed-1



Pan troglodytes (chimpanzee) EN1 XP_001172516.1
Mus musculus (house mouse) En1 NP_034263.2
Danio rerio (zebrafish) eng1b NP_001013516.1
Homo sapiens (human) EN1 NP_001417.3
Rattus norvegicus (Norway rat) En1 XP_001056699.2
Bos taurus (cattle) EN1 XP_003585804.1


Related articles in PubMed

Whole-genome sequencing identifies EN1 as a determinant of bone density and fracture.
Zheng HF, Forgetta V, Hsu YH, Estrada K, Rosello-Diez A, Leo PJ, Dahia CL, Park-Min KH, Tobias JH, Kooperberg C, Kleinman A, Styrkarsdottir U, Liu CT, Uggla C, Evans DS, Nielson CM, Walter K, Pettersson-Kymmer U, McCarthy S, Eriksson J, Kwan T, Jhamai M, Trajanoska K, Memari Y, Min J, Huang J, Danecek P, Wilmot B, Li R, Chou WC, Mokry LE, Moayyeri A, Claussnitzer M, Cheng CH, Cheung W, Medina-Gómez C, Ge B, Chen SH, Choi K, Oei L, Fraser J, Kraaij R, Hibbs MA, Gregson CL, Paquette D, Hofman A, Wibom C, Tranah GJ, Marshall M, Gardiner BB, Cremin K, Auer P, Hsu L, Ring S, Tung JY, Thorleifsson G, Enneman AW, van Schoor NM, de Groot LC, van der Velde N, Melin B, Kemp JP, Christiansen C, Sayers A, Zhou Y, Calderari S, van Rooij J, Carlson C, Peters U, Berlivet S, Dostie J, Uitterlinden AG, Williams SR, Farber C, Grinberg D, LaCroix AZ, Haessler J, Chasman DI, Giulianini F, Rose LM, Ridker PM, Eisman JA, Nguyen TV, Center JR, Nogues X, Garcia-Giralt N, Launer LL, Gudnason V, Mellström D, Vandenput L, Amin N, van Duijn CM, Karlsson MK, Ljunggren Ö, Svensson O, Hallmans G, Rousseau F, Giroux S, Bussière J, Arp PP, Koromani F, Prince RL, Lewis JR, Langdahl BL, Hermann AP, Jensen JE, Kaptoge S, Khaw KT, Reeve J, Formosa MM, Xuereb-Anastasi A, Åkesson K, McGuigan FE, Garg G, Olmos JM, Zarrabeitia MT, Riancho JA, Ralston SH, Alonso N, Jiang X, Goltzman D, Pastinen T, Grundberg E, Gauguier D, Orwoll ES, Karasik D, Davey-Smith G, , Smith AV, Siggeirsdottir K, Harris TB, Zillikens MC, van Meurs JB, Thorsteinsdottir U, Maurano MT, Timpson NJ, Soranzo N, Durbin R, Wilson SG, Ntzani EE, Brown MA, Stefansson K, Hinds DA, Spector T, Cupples LA, Ohlsson C, Greenwood CM, , Jackson RD, Rowe DW, Loomis CA, Evans DM, Ackert-Bicknell CL, Joyner AL, Duncan EL, Kiel DP, Rivadeneira F, Richards JB
Nature526(7571)112-7(2015 Oct)

Novel role of Engrailed 1 as a prosurvival transcription factor in basal-like breast cancer and engineering of interference peptides block its oncogenic function.
Beltran AS, Graves LM, Blancafort P
Oncogene33(39)4767-77(2014 Sep)

The lifelong maintenance of mesencephalic dopaminergic neurons by Nurr1 and engrailed.
Alavian KN, Jeddi S, Naghipour SI, Nabili P, Licznerski P, Tierney TS
Journal of biomedical science2127(2014 Apr)

Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of dopaminergic neurons.
Narytnyk A, Verdon B, Loughney A, Sweeney M, Clewes O, Taggart MJ, Sieber-Blum M
Stem cell reviews10(2)316-26(2014 Apr)

Wnt signaling in midbrain dopaminergic neuron development and regenerative medicine for Parkinson's disease.
Arenas E
Journal of molecular cell biology6(1)42-53(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

low-frequency non-coding variant near a novel locus, EN1, with an effect on bone mineral density--which was also associated with a decreased risk of fracture
Title: Whole-genome sequencing identifies EN1 as a determinant of bone density and fracture.

EN1 is an activator of intrinsic inflammatory pathways associated with prosurvival in basal-like breast cancer.
Title: Novel role of Engrailed 1 as a prosurvival transcription factor in basal-like breast cancer and engineering of interference peptides block its oncogenic function.

The current review describes the role of two such factors, Nurr1 and engrailed, in differentiation, maturation, and in normal physiological functions including acquisition of neurotransmitter identity.
Title: The lifelong maintenance of mesencephalic dopaminergic neurons by Nurr1 and engrailed.

EN1 is a biologic predictor of poor prognosis in patients with salivary adenoid cystic carcinoma.
Title: Developmental transcription factor EN1--a novel biomarker in human salivary gland adenoid cystic carcinoma.

Homeobox protein engrailed-1 protein regulates transcription of the utrophin gene.
Title: A method of utrophin up-regulation through RNAi-mediated knockdown of the transcription factor EN1.

The following EN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18410
NM_001426.3
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Homo sapiens engrailed homeobox 1 (EN1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
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$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18410
Clone ID Related Accession (Same CDS sequence) NM_001426.3
Accession Version NM_001426.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1179bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-09-16
Organism Homo sapiens(Human)
Product homeobox protein engrailed-1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012665.8. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2007 this sequence version replaced NM_001426.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAAGAAC AGCAGCCGGA ACCTAAAAGT CAGCGCGACT CGGCCCTCGG CGCGGCGGCG 
GCGGCGACTC CGGGCGGCCT CAGCCTGAGC CTCAGTCCGG GCGCCAGCGG CAGCAGCGGC
AGCGGCAGCG ATGGAGACAG CGTGCCGGTG TCCCCGCAGC CTGCGCCCCC CTCGCCGCCC
GCGGCGCCTT GCCTGCCGCC CCTGGCCCAC CACCCGCACC TCCCCCCACA CCCCCCGCCC
CCGCCGCCTC AGCATCTCGC GGCGCCTGCT CACCAGCCGC AGCCAGCGGC CCAGCTGCAC
CGCACCACCA ACTTTTTCAT CGACAACATC CTGAGGCCGG ACTTCGGCTG CAAAAAGGAG
CAGCCGCCAC CGCAGCTTCT GGTGGCTGCG GCGGCCAGAG GAGGCGCAGG AGGAGGAGGC
CGGGTCGAGC GTGACAGAGG CCAGACTGCC GCAGGTAGAG ACCCTGTCCA CCCGTTGGGC
ACCCGGGCGC CAGGCGCTGC CTCGCTCCTG TGCGCCCCGG ACGCGAACTG TGGCCCACCC
GACGGCTCCC AGCCAGCCGC CGCCGGCGCG GGCGCGTCTA AAGCTGGGAA CCCGGCTGCG
GCGGCGGCGG CGGCCGCGGC GGCAGTGGCG GCGGCGGCGG CGGCCGCAGC AGCCAAGCCC
TCGGACACCG GTGGCGGCGG CAGTGGAGGC GGCGCGGGGA GCCCCGGAGC GCAGGGCACC
AAATACCCGG AGCACGGCAA CCCGGCTATC CTACTTATGG GCTCAGCCAA CGGCGGGCCC
GTGGTCAAAA CTGACTCGCA GCAGCCTCTC GTATGGCCCG CCTGGGTGTA CTGCACACGT
TATTCGGATC GTCCATCCTC CGGTCCGCGC ACCAGGAAGC TGAAGAAGAA GAAGAACGAG
AAGGAGGACA AGCGGCCGCG GACCGCGTTC ACGGCCGAGC AGCTGCAGAG ACTCAAGGCG
GAGTTCCAGG CAAACCGCTA CATCACGGAG CAGCGGCGGC AGACCCTGGC CCAGGAACTC
AGCCTCAACG AGTCCCAGAT CAAGATCTGG TTCCAGAACA AGCGCGCCAA GATCAAGAAA
GCCACAGGCA TCAAGAACGG CCTGGCGCTG CACCTCATGG CCCAGGGACT GTACAACCAC
TCCACCACCA CGGTCCAGGA CAAAGACGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001417.3
CDS1017..2195
Misc Feature(1)897..899
Misc Feature(2)1923..2102
Misc Feature(3)order(1929..1940,1944..1946,1995..1997,2013..2015,2052..2054,2058..2063,2070..2075,2079..2087,2091..2096)
Misc Feature(4)1932..2093
Misc Feature(5)order(1932..1934,1941..1943,2061..2063,2070..2075,2082..2084)
Misc Feature(6)2097..2186
Exon (1)1..1878
Exon (2)1879..2962
Translation

Target ORF information:

RefSeq Version NM_001426.3
Organism Homo sapiens(Human)
Definition Homo sapiens engrailed homeobox 1 (EN1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001426.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAAGAAC AGCAGCCGGA ACCTAAAAGT CAGCGCGACT CGGCCCTCGG CGCGGCGGCG 
GCGGCGACTC CGGGCGGCCT CAGCCTGAGC CTCAGTCCGG GCGCCAGCGG CAGCAGCGGC
AGCGGCAGCG ATGGAGACAG CGTGCCGGTG TCCCCGCAGC CTGCGCCCCC CTCGCCGCCC
GCGGCGCCTT GCCTGCCGCC CCTGGCCCAC CACCCGCACC TCCCCCCACA CCCCCCGCCC
CCGCCGCCTC AGCATCTCGC GGCGCCTGCT CACCAGCCGC AGCCAGCGGC CCAGCTGCAC
CGCACCACCA ACTTTTTCAT CGACAACATC CTGAGGCCGG ACTTCGGCTG CAAAAAGGAG
CAGCCGCCAC CGCAGCTTCT GGTGGCTGCG GCGGCCAGAG GAGGCGCAGG AGGAGGAGGC
CGGGTCGAGC GTGACAGAGG CCAGACTGCC GCAGGTAGAG ACCCTGTCCA CCCGTTGGGC
ACCCGGGCGC CAGGCGCTGC CTCGCTCCTG TGCGCCCCGG ACGCGAACTG TGGCCCACCC
GACGGCTCCC AGCCAGCCGC CGCCGGCGCG GGCGCGTCTA AAGCTGGGAA CCCGGCTGCG
GCGGCGGCGG CGGCCGCGGC GGCAGTGGCG GCGGCGGCGG CGGCCGCAGC AGCCAAGCCC
TCGGACACCG GTGGCGGCGG CAGTGGAGGC GGCGCGGGGA GCCCCGGAGC GCAGGGCACC
AAATACCCGG AGCACGGCAA CCCGGCTATC CTACTTATGG GCTCAGCCAA CGGCGGGCCC
GTGGTCAAAA CTGACTCGCA GCAGCCTCTC GTATGGCCCG CCTGGGTGTA CTGCACACGT
TATTCGGATC GTCCATCCTC CGGTCCGCGC ACCAGGAAGC TGAAGAAGAA GAAGAACGAG
AAGGAGGACA AGCGGCCGCG GACCGCGTTC ACGGCCGAGC AGCTGCAGAG ACTCAAGGCG
GAGTTCCAGG CAAACCGCTA CATCACGGAG CAGCGGCGGC AGACCCTGGC CCAGGAACTC
AGCCTCAACG AGTCCCAGAT CAAGATCTGG TTCCAGAACA AGCGCGCCAA GATCAAGAAA
GCCACAGGCA TCAAGAACGG CCTGGCGCTG CACCTCATGG CCCAGGGACT GTACAACCAC
TCCACCACCA CGGTCCAGGA CAAAGACGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Whole-genome sequencing identifies EN1 as a determinant of bone density and fracture.
Nature526(7571)112-7(2015 Oct)
Zheng HF,Forgetta V,Hsu YH,Estrada K,Rosello-Diez A,Leo PJ,Dahia CL,Park-Min KH,Tobias JH,Kooperberg C,Kleinman A,Styrkarsdottir U,Liu CT,Uggla C,Evans DS,Nielson CM,Walter K,Pettersson-Kymmer U,McCarthy S,Eriksson J,Kwan T,Jhamai M,Trajanoska K,Memari Y,Min J,Huang J,Danecek P,Wilmot B,Li R,Chou WC,Mokry LE,Moayyeri A,Claussnitzer M,Cheng CH,Cheung W,Medina-Gómez C,Ge B,Chen SH,Choi K,Oei L,Fraser J,Kraaij R,Hibbs MA,Gregson CL,Paquette D,Hofman A,Wibom C,Tranah GJ,Marshall M,Gardiner BB,Cremin K,Auer P,Hsu L,Ring S,Tung JY,Thorleifsson G,Enneman AW,van Schoor NM,de Groot LC,van der Velde N,Melin B,Kemp JP,Christiansen C,Sayers A,Zhou Y,Calderari S,van Rooij J,Carlson C,Peters U,Berlivet S,Dostie J,Uitterlinden AG,Williams SR,Farber C,Grinberg D,LaCroix AZ,Haessler J,Chasman DI,Giulianini F,Rose LM,Ridker PM,Eisman JA,Nguyen TV,Center JR,Nogues X,Garcia-Giralt N,Launer LL,Gudnason V,Mellström D,Vandenput L,Amin N,van Duijn CM,Karlsson MK,Ljunggren Ö,Svensson O,Hallmans G,Rousseau F,Giroux S,Bussière J,Arp PP,Koromani F,Prince RL,Lewis JR,Langdahl BL,Hermann AP,Jensen JE,Kaptoge S,Khaw KT,Reeve J,Formosa MM,Xuereb-Anastasi A,Åkesson K,McGuigan FE,Garg G,Olmos JM,Zarrabeitia MT,Riancho JA,Ralston SH,Alonso N,Jiang X,Goltzman D,Pastinen T,Grundberg E,Gauguier D,Orwoll ES,Karasik D,Davey-Smith G,,Smith AV,Siggeirsdottir K,Harris TB,Zillikens MC,van Meurs JB,Thorsteinsdottir U,Maurano MT,Timpson NJ,Soranzo N,Durbin R,Wilson SG,Ntzani EE,Brown MA,Stefansson K,Hinds DA,Spector T,Cupples LA,Ohlsson C,Greenwood CM,,Jackson RD,Rowe DW,Loomis CA,Evans DM,Ackert-Bicknell CL,Joyner AL,Duncan EL,Kiel DP,Rivadeneira F,Richards JB


book

Novel role of Engrailed 1 as a prosurvival transcription factor in basal-like breast cancer and engineering of interference peptides block its oncogenic function.
Oncogene33(39)4767-77(2014 Sep)
Beltran AS,Graves LM,Blancafort P


book

The lifelong maintenance of mesencephalic dopaminergic neurons by Nurr1 and engrailed.
Journal of biomedical science2127(2014 Apr)
Alavian KN,Jeddi S,Naghipour SI,Nabili P,Licznerski P,Tierney TS


book

Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of dopaminergic neurons.
Stem cell reviews10(2)316-26(2014 Apr)
Narytnyk A,Verdon B,Loughney A,Sweeney M,Clewes O,Taggart MJ,Sieber-Blum M


book

Wnt signaling in midbrain dopaminergic neuron development and regenerative medicine for Parkinson's disease.
Journal of molecular cell biology6(1)42-53(2014 Feb)
Arenas E


 
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