×

EPO cDNA ORF clone, Homo sapiens(Human)

Gene Symbol EPO
Entrez Gene ID 2056
Full Name erythropoietin
Synonyms EP,MVCD2
General protein information
Preferred Names

erythropoietin

Names

erythropoietin
epoetin

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7q22.1

Summary This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008].
Disorder MIM:

133170

Disorder Html: {Microvascular complications of diabetes 2}, 612623 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000799.2 NP_000790.2 erythropoietin precursor



Macaca mulatta (rhesus monkey) EPO NP_001036201.1
Danio rerio (zebrafish) epo NP_001033098.1
Danio rerio (zebrafish) LOC100002479 XP_001342254.1
Bos taurus (cattle) EPO NP_776334.1
Pan troglodytes (chimpanzee) EPO XP_519268.2
Mus musculus (house mouse) Epo NP_031968.1
Xenopus tropicalis (tropical clawed frog) epo NP_001233194.1
Homo sapiens (human) EPO NP_000790.2
Canis lupus familiaris (dog) EPO NP_001006647.1
Rattus norvegicus (Norway rat) Epo NP_058697.1


Related articles in PubMed

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.
Kim AR, Ulirsch JC, Wilmes S, Unal E, Moraga I, Karakukcu M, Yuan D, Kazerounian S, Abdulhay NJ, King DS, Gupta N, Gabriel SB, Lander ES, Patiroglu T, Ozcan A, Ozdemir MA, Garcia KC, Piehler J, Gazda HT, Klein DE, Sankaran VG
Cell168(6)1053-1064.e15(2017 Mar)

Erythropoietin induces production of hepatocyte growth factor from bone marrow mesenchymal stem cells in vitro.
Tari K, Atashi A, Kaviani S, AkhavanRahnama M, Anbarlou A, Mossahebi-Mohammadi M
Biologicals : journal of the International Association of Biological Standardization4515-19(2017 Jan)

Erythropoietin accelerates tumor growth through increase of erythropoietin receptor (EpoR) as well as by the stimulation of angiogenesis in DLD-1 and Ht-29 xenografts.
Tankiewicz-Kwedlo A, Hermanowicz J, Surażynski A, Rożkiewicz D, Pryczynicz A, Domaniewski T, Pawlak K, Kemona A, Pawlak D
Molecular and cellular biochemistry421(1-2)1-18(2016 Oct)

Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins.
Stoyanoff TR, Rodríguez JP, Todaro JS, Espada JD, Colavita JP, Brandan NC, Torres AM, Aguirre MV
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)13581-13593(2016 Oct)

Erythropoietin exerts direct immunomodulatory effects on the cytokine production by activated human T-lymphocytes.
Todosenko NM, Shmarov VA, Malashchenko VV, Meniailo ME, Melashchenko OB, Gazatova ND, Goncharov AG, Seledtsov VI
International immunopharmacology36277-281(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pharmacokinetic animal studies revealed strongly 15.6-fold plasma half-life extension for the PASylated EPO (83.16 +/- 13.28 h) in comparison to epoetin alpha (8.5 +/- 2.4 h) and darbepoetin alpha (25.3 +/- 2.2h).
Title: Molecular Design, Expression and Evaluation of PASylated Human Recombinant Erythropoietin with Enhanced Functional Properties.

EPO may play an important role in stem cell mobilization through up regulating HGF in mesenchymal stem cells and inducing migration of hematopoietic stem/progenitor cells
Title: Erythropoietin induces production of hepatocyte growth factor from bone marrow mesenchymal stem cells in vitro.

CK1delta enhances EPO secretion from liver cancer cells under hypoxia by modifying HIF-2alpha and promoting its nuclear accumulation.
Title: HIF-2α phosphorylation by CK1δ promotes erythropoietin secretion in liver cancer cells under hypoxia.

These results suggest that both EpoR-positive and EpoR-negative cancer cells could be regulated by exogenous Epo. However, an increased response to erythropoietin was observed in the EpoR-positive cells. Thus, erythropoietin increases the risk of tumor progression in colon cancer and should not be used to treat anemia in this type of cancer.
Title: Erythropoietin accelerates tumor growth through increase of erythropoietin receptor (EpoR) as well as by the stimulation of angiogenesis in DLD-1 and Ht-29 xenografts.

EPO levels were also found correlated positively with heme, TNF-alpha, IL-10, IP-10 and MCP-1 during cerebral malaria.
Title: Erythropoietin Levels Increase during Cerebral Malaria and Correlate with Heme, Interleukin-10 and Tumor Necrosis Factor-Alpha in India.

The following EPO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20340 NM_000799.2
Latest version!
Homo sapiens erythropoietin (EPO), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20340
Clone ID Related Accession (Same CDS sequence) NM_000799.2
Accession Version NM_000799.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product erythropoietin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X02157.1 and S65458.1. This sequence is a reference standard in the RefSeqGene project. On Apr 6, 2005 this sequence version replaced NM_000799.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X02157.1, BC093628.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA2159368 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGGGGTGC ACGAATGTCC TGCCTGGCTG TGGCTTCTCC TGTCCCTGCT GTCGCTCCCT 
CTGGGCCTCC CAGTCCTGGG CGCCCCACCA CGCCTCATCT GTGACAGCCG AGTCCTGGAG
AGGTACCTCT TGGAGGCCAA GGAGGCCGAG AATATCACGA CGGGCTGTGC TGAACACTGC
AGCTTGAATG AGAATATCAC TGTCCCAGAC ACCAAAGTTA ATTTCTATGC CTGGAAGAGG
ATGGAGGTCG GGCAGCAGGC CGTAGAAGTC TGGCAGGGCC TGGCCCTGCT GTCGGAAGCT
GTCCTGCGGG GCCAGGCCCT GTTGGTCAAC TCTTCCCAGC CGTGGGAGCC CCTGCAGCTG
CATGTGGATA AAGCCGTCAG TGGCCTTCGC AGCCTCACCA CTCTGCTTCG GGCTCTGGGA
GCCCAGAAGG AAGCCATCTC CCCTCCAGAT GCGGCCTCAG CTGCTCCACT CCGAACAATC
ACTGCTGACA CTTTCCGCAA ACTCTTCCGA GTCTACTCCA ATTTCCTCCG GGGAAAGCTG
AAGCTGTACA CAGGGGAGGC CTGCAGGACA GGGGACAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000790.2
CDS182..763
Misc Feature(1)116..118
Misc Feature(2)272..757
Misc Feature(3)order(281..283,743..745)
Misc Feature(4)332..334
Misc Feature(5)order(347..349,359..361)
Misc Feature(6)374..376
Misc Feature(7)509..511
Misc Feature(8)638..640
Exon (1)1..194
Exon (2)195..340
Exon (3)341..427
Exon (4)428..607
Exon (5)608..1330
Translation

Target ORF information:

RefSeq Version NM_000799.2
Organism Homo sapiens(Human)
Definition Homo sapiens erythropoietin (EPO), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000799.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGGGTGC ACGAATGTCC TGCCTGGCTG TGGCTTCTCC TGTCCCTGCT GTCGCTCCCT 
CTGGGCCTCC CAGTCCTGGG CGCCCCACCA CGCCTCATCT GTGACAGCCG AGTCCTGGAG
AGGTACCTCT TGGAGGCCAA GGAGGCCGAG AATATCACGA CGGGCTGTGC TGAACACTGC
AGCTTGAATG AGAATATCAC TGTCCCAGAC ACCAAAGTTA ATTTCTATGC CTGGAAGAGG
ATGGAGGTCG GGCAGCAGGC CGTAGAAGTC TGGCAGGGCC TGGCCCTGCT GTCGGAAGCT
GTCCTGCGGG GCCAGGCCCT GTTGGTCAAC TCTTCCCAGC CGTGGGAGCC CCTGCAGCTG
CATGTGGATA AAGCCGTCAG TGGCCTTCGC AGCCTCACCA CTCTGCTTCG GGCTCTGGGA
GCCCAGAAGG AAGCCATCTC CCCTCCAGAT GCGGCCTCAG CTGCTCCACT CCGAACAATC
ACTGCTGACA CTTTCCGCAA ACTCTTCCGA GTCTACTCCA ATTTCCTCCG GGGAAAGCTG
AAGCTGTACA CAGGGGAGGC CTGCAGGACA GGGGACAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.
Cell168(6)1053-1064.e15(2017 Mar)
Kim AR,Ulirsch JC,Wilmes S,Unal E,Moraga I,Karakukcu M,Yuan D,Kazerounian S,Abdulhay NJ,King DS,Gupta N,Gabriel SB,Lander ES,Patiroglu T,Ozcan A,Ozdemir MA,Garcia KC,Piehler J,Gazda HT,Klein DE,Sankaran VG


book

Erythropoietin induces production of hepatocyte growth factor from bone marrow mesenchymal stem cells in vitro.
Biologicals : journal of the International Association of Biological Standardization4515-19(2017 Jan)
Tari K,Atashi A,Kaviani S,AkhavanRahnama M,Anbarlou A,Mossahebi-Mohammadi M


book

Erythropoietin accelerates tumor growth through increase of erythropoietin receptor (EpoR) as well as by the stimulation of angiogenesis in DLD-1 and Ht-29 xenografts.
Molecular and cellular biochemistry421(1-2)1-18(2016 Oct)
Tankiewicz-Kwedlo A,Hermanowicz J,Surażynski A,Rożkiewicz D,Pryczynicz A,Domaniewski T,Pawlak K,Kemona A,Pawlak D


book

Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)13581-13593(2016 Oct)
Stoyanoff TR,Rodríguez JP,Todaro JS,Espada JD,Colavita JP,Brandan NC,Torres AM,Aguirre MV


book

Erythropoietin exerts direct immunomodulatory effects on the cytokine production by activated human T-lymphocytes.
International immunopharmacology36277-281(2016 Jul)
Todosenko NM,Shmarov VA,Malashchenko VV,Meniailo ME,Melashchenko OB,Gazatova ND,Goncharov AG,Seledtsov VI


 
*
*
*
*