GLI1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following GLI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu22564 NM_005269.3
Latest version!
Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu23011 NM_001160045.1
Latest version!
Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu22564 XM_011538189.2
Latest version!
Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu23011 XM_005268799.2
Latest version!
Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20556 NM_001167609.1
Latest version!
Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $755.30
$1079.00
OHu57981 XM_011538190.2
Latest version!
Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu22564 XM_011538189.1 Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu22564 NM_005269.2 Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu57981 XM_011538190.1 Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu22564
    Clone ID Related Accession (Same CDS sequence) XM_011538189.1 , XM_011538189.2 , NM_005269.2 , NM_005269.3
    Accession Version NM_005269.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-28
    Organism Homo sapiens(human)
    Product zinc finger protein GLI1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X07384.1, AC022506.38 and BC013000.2. This sequence is a reference standard in the RefSeqGene project. On Mar 11, 2009 this sequence version replaced NM_005269.1. Transcript Variant: This variant (1) encodes the full length protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X07384.1, BC013000.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCGTATGTAT GTAAGCTCCC TGGCTGCACC AAACGCTATA CAGATCCTAG CTCGCTGCGA
    AAACATGTCA AGACAGTGCA TGGTCCTGAC GCCCATGTGA CCAAACGGCA CCGTGGGGAT
    GGCCCCCTGC CTCGGGCACC ATCCATTTCT ACAGTGGAGC CCAAGAGGGA GCGGGAAGGA
    GGTCCCATCA GGGAGGAAAG CAGACTGACT GTGCCAGAGG GTGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CGGCAGGGAG TGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGCAAT GCAGGGGGCA GCACTGAAGA CCTCTCCAGC
    TTGGACGAGG GACCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAATAGGGA CCCGGGGTCT CAAACTGCCC
    AGCTTGTCCC ACACCGGTAC CACTGTGTCC CGCCGCGTGG GCCCCCCAGT CTCTCTTGAA
    CGCCGCAGCA GCAGCTCCAG CAGCATCAGC TCTGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCTC CTTTCCCCCC TGGCTCCCCA CCAGAGAATG GAGCATCCTC CCTGCCTGGC
    CTTATGCCTG CCCAGCACTA CCTGCTTCGG GCAAGATATG CTTCAGCCAG AGGGGGTGGT
    ACTTCGCCCA CTGCAGCATC CAGCCTGGAT CGGATAGGTG GTCTTCCCAT GCCTCCTTGG
    AGAAGCCGAG CCGAGTATCC AGGATACAAC CCCAATGCAG GGGTCACCCG GAGGGCCAGT
    GACCCAGCCC AGGCTGCTGA CCGTCCTGCT CCAGCTAGAG TCCAGAGGTT CAAGAGCCTG
    GGCTGTGTCC ATACCCCACC CACTGTGGCA GGGGGAGGAC AGAACTTTGA TCCTTACCTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGCTGC CATGGATGCT
    AGAGGGCTAC AGGAAGAGCC AGAAGTTGGG ACCTCCATGG TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTAC TGATACTCTG GGATATGGGG GACCTGAAGG GGCAGCAGCT
    GAGCCTTATG GAGCGAGGGG TCCAGGCTCT CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGCC TCATATCCTG ACCCCACCCA AGAAACATGG
    GGTGAGTTCC CTTCCCACTC TGGGCTGTAC CCAGGCCCCA AGGCTCTAGG TGGAACCTAC
    AGCCAGTGTC CTCGACTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGC
    CCAGTGGGGT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAGTGAGGGG
    CCCCCACATC CACAGCCTCT CTTTTCCCAT TACCCCCAGC CCTCTCCTCC CCAATATCTC
    CAGTCAGGCC CCTATACCCA GCCACCCCCT GATTATCTTC CTTCAGAACC CAGGCCTTGC
    CTGGACTTTG ATTCCCCCAC CCATTCCACA GGGCAGCTCA AGGCTCAGCT TGTGTGTAAT
    TATGTTCAAT CTCAACAGGA GCTACTGTGG GAGGGTGGGG GCAGGGAAGA TGCCCCCGCC
    CAGGAACCTT CCTACCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTTAG CCCAAGCCGT
    GCTAAAGCTC CAGTGAACAC ATATGGACCT GGCTTTGGAC CCAACTTGCC CAATCACAAG
    TCAGGTTCCT ATCCCACCCC TTCACCATGC CATGAAAATT TTGTAGTGGG GGCAAATAGG
    GCTTCACATA GGGCAGCAGC ACCACCTCGA CTTCTGCCCC CATTGCCCAC TTGCTATGGG
    CCTCTCAAAG TGGGAGGCAC AAACCCCAGC TGTGGTCATC CTGAGGTGGG CAGGCTAGGA
    GGGGGTCCTG CCTTGTACCC TCCTCCCGAA GGACAGGTAT GTAACCCCCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGCCCCA GGGGCTGAGT
    CCTCCTCCTT CCCATGATCA GCGGGGCAGC TCTGGACATA CCCCACCTCC CTCTGGGCCC
    CCCAACATGG CTGTGGGCAA CATGAGTGTC TTACTGAGAT CCCTACCTGG GGAAACAGAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005260.1
    CDS79..3399
    Translation

    Target ORF information:

    RefSeq Version NM_005269.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005269.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCGTATGTAT GTAAGCTCCC TGGCTGCACC AAACGCTATA CAGATCCTAG CTCGCTGCGA
    AAACATGTCA AGACAGTGCA TGGTCCTGAC GCCCATGTGA CCAAACGGCA CCGTGGGGAT
    GGCCCCCTGC CTCGGGCACC ATCCATTTCT ACAGTGGAGC CCAAGAGGGA GCGGGAAGGA
    GGTCCCATCA GGGAGGAAAG CAGACTGACT GTGCCAGAGG GTGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CGGCAGGGAG TGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGCAAT GCAGGGGGCA GCACTGAAGA CCTCTCCAGC
    TTGGACGAGG GACCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAATAGGGA CCCGGGGTCT CAAACTGCCC
    AGCTTGTCCC ACACCGGTAC CACTGTGTCC CGCCGCGTGG GCCCCCCAGT CTCTCTTGAA
    CGCCGCAGCA GCAGCTCCAG CAGCATCAGC TCTGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCTC CTTTCCCCCC TGGCTCCCCA CCAGAGAATG GAGCATCCTC CCTGCCTGGC
    CTTATGCCTG CCCAGCACTA CCTGCTTCGG GCAAGATATG CTTCAGCCAG AGGGGGTGGT
    ACTTCGCCCA CTGCAGCATC CAGCCTGGAT CGGATAGGTG GTCTTCCCAT GCCTCCTTGG
    AGAAGCCGAG CCGAGTATCC AGGATACAAC CCCAATGCAG GGGTCACCCG GAGGGCCAGT
    GACCCAGCCC AGGCTGCTGA CCGTCCTGCT CCAGCTAGAG TCCAGAGGTT CAAGAGCCTG
    GGCTGTGTCC ATACCCCACC CACTGTGGCA GGGGGAGGAC AGAACTTTGA TCCTTACCTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGCTGC CATGGATGCT
    AGAGGGCTAC AGGAAGAGCC AGAAGTTGGG ACCTCCATGG TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTAC TGATACTCTG GGATATGGGG GACCTGAAGG GGCAGCAGCT
    GAGCCTTATG GAGCGAGGGG TCCAGGCTCT CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGCC TCATATCCTG ACCCCACCCA AGAAACATGG
    GGTGAGTTCC CTTCCCACTC TGGGCTGTAC CCAGGCCCCA AGGCTCTAGG TGGAACCTAC
    AGCCAGTGTC CTCGACTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGC
    CCAGTGGGGT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAGTGAGGGG
    CCCCCACATC CACAGCCTCT CTTTTCCCAT TACCCCCAGC CCTCTCCTCC CCAATATCTC
    CAGTCAGGCC CCTATACCCA GCCACCCCCT GATTATCTTC CTTCAGAACC CAGGCCTTGC
    CTGGACTTTG ATTCCCCCAC CCATTCCACA GGGCAGCTCA AGGCTCAGCT TGTGTGTAAT
    TATGTTCAAT CTCAACAGGA GCTACTGTGG GAGGGTGGGG GCAGGGAAGA TGCCCCCGCC
    CAGGAACCTT CCTACCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTTAG CCCAAGCCGT
    GCTAAAGCTC CAGTGAACAC ATATGGACCT GGCTTTGGAC CCAACTTGCC CAATCACAAG
    TCAGGTTCCT ATCCCACCCC TTCACCATGC CATGAAAATT TTGTAGTGGG GGCAAATAGG
    GCTTCACATA GGGCAGCAGC ACCACCTCGA CTTCTGCCCC CATTGCCCAC TTGCTATGGG
    CCTCTCAAAG TGGGAGGCAC AAACCCCAGC TGTGGTCATC CTGAGGTGGG CAGGCTAGGA
    GGGGGTCCTG CCTTGTACCC TCCTCCCGAA GGACAGGTAT GTAACCCCCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGCCCCA GGGGCTGAGT
    CCTCCTCCTT CCCATGATCA GCGGGGCAGC TCTGGACATA CCCCACCTCC CTCTGGGCCC
    CCCAACATGG CTGTGGGCAA CATGAGTGTC TTACTGAGAT CCCTACCTGG GGAAACAGAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22564
    Clone ID Related Accession (Same CDS sequence) XM_011538189.1 , XM_011538189.2 , NM_005269.2 , NM_005269.3
    Accession Version NM_005269.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-09
    Organism Homo sapiens(human)
    Product zinc finger protein GLI1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022506.38, X07384.1 and BC013000.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005269.2. Transcript Variant: This variant (1) encodes the full length protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013000.2, X07384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000228682.7/ ENSP00000228682.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCGTATGTAT GTAAGCTCCC TGGCTGCACC AAACGCTATA CAGATCCTAG CTCGCTGCGA
    AAACATGTCA AGACAGTGCA TGGTCCTGAC GCCCATGTGA CCAAACGGCA CCGTGGGGAT
    GGCCCCCTGC CTCGGGCACC ATCCATTTCT ACAGTGGAGC CCAAGAGGGA GCGGGAAGGA
    GGTCCCATCA GGGAGGAAAG CAGACTGACT GTGCCAGAGG GTGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CGGCAGGGAG TGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGCAAT GCAGGGGGCA GCACTGAAGA CCTCTCCAGC
    TTGGACGAGG GACCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAATAGGGA CCCGGGGTCT CAAACTGCCC
    AGCTTGTCCC ACACCGGTAC CACTGTGTCC CGCCGCGTGG GCCCCCCAGT CTCTCTTGAA
    CGCCGCAGCA GCAGCTCCAG CAGCATCAGC TCTGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCTC CTTTCCCCCC TGGCTCCCCA CCAGAGAATG GAGCATCCTC CCTGCCTGGC
    CTTATGCCTG CCCAGCACTA CCTGCTTCGG GCAAGATATG CTTCAGCCAG AGGGGGTGGT
    ACTTCGCCCA CTGCAGCATC CAGCCTGGAT CGGATAGGTG GTCTTCCCAT GCCTCCTTGG
    AGAAGCCGAG CCGAGTATCC AGGATACAAC CCCAATGCAG GGGTCACCCG GAGGGCCAGT
    GACCCAGCCC AGGCTGCTGA CCGTCCTGCT CCAGCTAGAG TCCAGAGGTT CAAGAGCCTG
    GGCTGTGTCC ATACCCCACC CACTGTGGCA GGGGGAGGAC AGAACTTTGA TCCTTACCTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGCTGC CATGGATGCT
    AGAGGGCTAC AGGAAGAGCC AGAAGTTGGG ACCTCCATGG TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTAC TGATACTCTG GGATATGGGG GACCTGAAGG GGCAGCAGCT
    GAGCCTTATG GAGCGAGGGG TCCAGGCTCT CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGCC TCATATCCTG ACCCCACCCA AGAAACATGG
    GGTGAGTTCC CTTCCCACTC TGGGCTGTAC CCAGGCCCCA AGGCTCTAGG TGGAACCTAC
    AGCCAGTGTC CTCGACTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGC
    CCAGTGGGGT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAGTGAGGGG
    CCCCCACATC CACAGCCTCT CTTTTCCCAT TACCCCCAGC CCTCTCCTCC CCAATATCTC
    CAGTCAGGCC CCTATACCCA GCCACCCCCT GATTATCTTC CTTCAGAACC CAGGCCTTGC
    CTGGACTTTG ATTCCCCCAC CCATTCCACA GGGCAGCTCA AGGCTCAGCT TGTGTGTAAT
    TATGTTCAAT CTCAACAGGA GCTACTGTGG GAGGGTGGGG GCAGGGAAGA TGCCCCCGCC
    CAGGAACCTT CCTACCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTTAG CCCAAGCCGT
    GCTAAAGCTC CAGTGAACAC ATATGGACCT GGCTTTGGAC CCAACTTGCC CAATCACAAG
    TCAGGTTCCT ATCCCACCCC TTCACCATGC CATGAAAATT TTGTAGTGGG GGCAAATAGG
    GCTTCACATA GGGCAGCAGC ACCACCTCGA CTTCTGCCCC CATTGCCCAC TTGCTATGGG
    CCTCTCAAAG TGGGAGGCAC AAACCCCAGC TGTGGTCATC CTGAGGTGGG CAGGCTAGGA
    GGGGGTCCTG CCTTGTACCC TCCTCCCGAA GGACAGGTAT GTAACCCCCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGCCCCA GGGGCTGAGT
    CCTCCTCCTT CCCATGATCA GCGGGGCAGC TCTGGACATA CCCCACCTCC CTCTGGGCCC
    CCCAACATGG CTGTGGGCAA CATGAGTGTC TTACTGAGAT CCCTACCTGG GGAAACAGAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005260.1
    CDS445..3765
    Translation

    Target ORF information:

    RefSeq Version NM_005269.3
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005269.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCGTATGTAT GTAAGCTCCC TGGCTGCACC AAACGCTATA CAGATCCTAG CTCGCTGCGA
    AAACATGTCA AGACAGTGCA TGGTCCTGAC GCCCATGTGA CCAAACGGCA CCGTGGGGAT
    GGCCCCCTGC CTCGGGCACC ATCCATTTCT ACAGTGGAGC CCAAGAGGGA GCGGGAAGGA
    GGTCCCATCA GGGAGGAAAG CAGACTGACT GTGCCAGAGG GTGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CGGCAGGGAG TGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGCAAT GCAGGGGGCA GCACTGAAGA CCTCTCCAGC
    TTGGACGAGG GACCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAATAGGGA CCCGGGGTCT CAAACTGCCC
    AGCTTGTCCC ACACCGGTAC CACTGTGTCC CGCCGCGTGG GCCCCCCAGT CTCTCTTGAA
    CGCCGCAGCA GCAGCTCCAG CAGCATCAGC TCTGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCTC CTTTCCCCCC TGGCTCCCCA CCAGAGAATG GAGCATCCTC CCTGCCTGGC
    CTTATGCCTG CCCAGCACTA CCTGCTTCGG GCAAGATATG CTTCAGCCAG AGGGGGTGGT
    ACTTCGCCCA CTGCAGCATC CAGCCTGGAT CGGATAGGTG GTCTTCCCAT GCCTCCTTGG
    AGAAGCCGAG CCGAGTATCC AGGATACAAC CCCAATGCAG GGGTCACCCG GAGGGCCAGT
    GACCCAGCCC AGGCTGCTGA CCGTCCTGCT CCAGCTAGAG TCCAGAGGTT CAAGAGCCTG
    GGCTGTGTCC ATACCCCACC CACTGTGGCA GGGGGAGGAC AGAACTTTGA TCCTTACCTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGCTGC CATGGATGCT
    AGAGGGCTAC AGGAAGAGCC AGAAGTTGGG ACCTCCATGG TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTAC TGATACTCTG GGATATGGGG GACCTGAAGG GGCAGCAGCT
    GAGCCTTATG GAGCGAGGGG TCCAGGCTCT CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGCC TCATATCCTG ACCCCACCCA AGAAACATGG
    GGTGAGTTCC CTTCCCACTC TGGGCTGTAC CCAGGCCCCA AGGCTCTAGG TGGAACCTAC
    AGCCAGTGTC CTCGACTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGC
    CCAGTGGGGT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAGTGAGGGG
    CCCCCACATC CACAGCCTCT CTTTTCCCAT TACCCCCAGC CCTCTCCTCC CCAATATCTC
    CAGTCAGGCC CCTATACCCA GCCACCCCCT GATTATCTTC CTTCAGAACC CAGGCCTTGC
    CTGGACTTTG ATTCCCCCAC CCATTCCACA GGGCAGCTCA AGGCTCAGCT TGTGTGTAAT
    TATGTTCAAT CTCAACAGGA GCTACTGTGG GAGGGTGGGG GCAGGGAAGA TGCCCCCGCC
    CAGGAACCTT CCTACCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTTAG CCCAAGCCGT
    GCTAAAGCTC CAGTGAACAC ATATGGACCT GGCTTTGGAC CCAACTTGCC CAATCACAAG
    TCAGGTTCCT ATCCCACCCC TTCACCATGC CATGAAAATT TTGTAGTGGG GGCAAATAGG
    GCTTCACATA GGGCAGCAGC ACCACCTCGA CTTCTGCCCC CATTGCCCAC TTGCTATGGG
    CCTCTCAAAG TGGGAGGCAC AAACCCCAGC TGTGGTCATC CTGAGGTGGG CAGGCTAGGA
    GGGGGTCCTG CCTTGTACCC TCCTCCCGAA GGACAGGTAT GTAACCCCCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGCCCCA GGGGCTGAGT
    CCTCCTCCTT CCCATGATCA GCGGGGCAGC TCTGGACATA CCCCACCTCC CTCTGGGCCC
    CCCAACATGG CTGTGGGCAA CATGAGTGTC TTACTGAGAT CCCTACCTGG GGAAACAGAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23011
    Clone ID Related Accession (Same CDS sequence) NM_001160045.1 , XM_005268799.2
    Accession Version NM_001160045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product zinc finger protein GLI1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK297899.1, BC013000.2 and AC022506.38. Transcript Variant: This variant (1) omits the first two coding exons, compared to variant 1, resulting in transcription initiation from an internal AUG site and a N-terminally truncated protein isoform (2), also know as GLI1DeltaN. This variant reflects the transcript and protein described by Shimokawa (PubMed ID 18378682). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGCCCAT CTCTGGGATT CCCAGCCCAG ATGAATCACC AAAAAGGGCC CTCGCCTTCC 
    TTTGGGGTCC AGCCTTGTGG TCCCCATGAC TCTGCCCGGG GTGGGATGAT CCCACATCCT
    CAGTCCCGGG GACCCTTCCC AACTTGCCAG CTGAAGTCTG AGCTGGACAT GCTGGTTGGC
    AAGTGCCGGG AGGAACCCTT GGAAGGTGAT ATGTCCAGCC CCAACTCCAC AGGCATACAG
    GATCCCCTGT TGGGGATGCT GGATGGGCGG GAGGACCTCG AGAGAGAGGA GAAGCGTGAG
    CCTGAATCTG TGTATGAAAC TGACTGCCGT TGGGATGGCT GCAGCCAGGA ATTTGACTCC
    CAAGAGCAGC TGGTGCACCA CATCAACAGC GAGCACATCC ACGGGGAGCG GAAGGAGTTC
    GTGTGCCACT GGGGGGGCTG CTCCAGGGAG CTGAGGCCCT TCAAAGCCCA GTACATGCTG
    GTGGTTCACA TGCGCAGACA CACTGGCGAG AAGCCACACA AGTGCACGTT TGAAGGGTGC
    CGGAAGTCAT ACTCACGCCT CGAAAACCTG AAGACGCACC TGCGGTCACA CACGGGTGAG
    AAGCCATACA TGTGTGAGCA CGAGGGCTGC AGTAAAGCCT TCAGCAATGC CAGTGACCGA
    GCCAAGCACC AGAATCGGAC CCATTCCAAT GAGAAGCCGT ATGTATGTAA GCTCCCTGGC
    TGCACCAAAC GCTATACAGA TCCTAGCTCG CTGCGAAAAC ATGTCAAGAC AGTGCATGGT
    CCTGACGCCC ATGTGACCAA ACGGCACCGT GGGGATGGCC CCCTGCCTCG GGCACCATCC
    ATTTCTACAG TGGAGCCCAA GAGGGAGCGG GAAGGAGGTC CCATCAGGGA GGAAAGCAGA
    CTGACTGTGC CAGAGGGTGC CATGAAGCCA CAGCCAAGCC CTGGGGCCCA GTCATCCTGC
    AGCAGTGACC ACTCCCCGGC AGGGAGTGCA GCCAATACAG ACAGTGGTGT GGAAATGACT
    GGCAATGCAG GGGGCAGCAC TGAAGACCTC TCCAGCTTGG ACGAGGGACC TTGCATTGCT
    GGCACTGGTC TGTCCACTCT TCGCCGCCTT GAGAACCTCA GGCTGGACCA GCTACATCAA
    CTCCGGCCAA TAGGGACCCG GGGTCTCAAA CTGCCCAGCT TGTCCCACAC CGGTACCACT
    GTGTCCCGCC GCGTGGGCCC CCCAGTCTCT CTTGAACGCC GCAGCAGCAG CTCCAGCAGC
    ATCAGCTCTG CCTATACTGT CAGCCGCCGC TCCTCCCTGG CCTCTCCTTT CCCCCCTGGC
    TCCCCACCAG AGAATGGAGC ATCCTCCCTG CCTGGCCTTA TGCCTGCCCA GCACTACCTG
    CTTCGGGCAA GATATGCTTC AGCCAGAGGG GGTGGTACTT CGCCCACTGC AGCATCCAGC
    CTGGATCGGA TAGGTGGTCT TCCCATGCCT CCTTGGAGAA GCCGAGCCGA GTATCCAGGA
    TACAACCCCA ATGCAGGGGT CACCCGGAGG GCCAGTGACC CAGCCCAGGC TGCTGACCGT
    CCTGCTCCAG CTAGAGTCCA GAGGTTCAAG AGCCTGGGCT GTGTCCATAC CCCACCCACT
    GTGGCAGGGG GAGGACAGAA CTTTGATCCT TACCTCCCAA CCTCTGTCTA CTCACCACAG
    CCCCCCAGCA TCACTGAGAA TGCTGCCATG GATGCTAGAG GGCTACAGGA AGAGCCAGAA
    GTTGGGACCT CCATGGTGGG CAGTGGTCTG AACCCCTATA TGGACTTCCC ACCTACTGAT
    ACTCTGGGAT ATGGGGGACC TGAAGGGGCA GCAGCTGAGC CTTATGGAGC GAGGGGTCCA
    GGCTCTCTGC CTCTTGGGCC TGGTCCACCC ACCAACTATG GCCCCAACCC CTGTCCCCAG
    CAGGCCTCAT ATCCTGACCC CACCCAAGAA ACATGGGGTG AGTTCCCTTC CCACTCTGGG
    CTGTACCCAG GCCCCAAGGC TCTAGGTGGA ACCTACAGCC AGTGTCCTCG ACTTGAACAT
    TATGGACAAG TGCAAGTCAA GCCAGAACAG GGGTGCCCAG TGGGGTCTGA CTCCACAGGA
    CTGGCACCCT GCCTCAATGC CCACCCCAGT GAGGGGCCCC CACATCCACA GCCTCTCTTT
    TCCCATTACC CCCAGCCCTC TCCTCCCCAA TATCTCCAGT CAGGCCCCTA TACCCAGCCA
    CCCCCTGATT ATCTTCCTTC AGAACCCAGG CCTTGCCTGG ACTTTGATTC CCCCACCCAT
    TCCACAGGGC AGCTCAAGGC TCAGCTTGTG TGTAATTATG TTCAATCTCA ACAGGAGCTA
    CTGTGGGAGG GTGGGGGCAG GGAAGATGCC CCCGCCCAGG AACCTTCCTA CCAGAGTCCC
    AAGTTTCTGG GGGGTTCCCA GGTTAGCCCA AGCCGTGCTA AAGCTCCAGT GAACACATAT
    GGACCTGGCT TTGGACCCAA CTTGCCCAAT CACAAGTCAG GTTCCTATCC CACCCCTTCA
    CCATGCCATG AAAATTTTGT AGTGGGGGCA AATAGGGCTT CACATAGGGC AGCAGCACCA
    CCTCGACTTC TGCCCCCATT GCCCACTTGC TATGGGCCTC TCAAAGTGGG AGGCACAAAC
    CCCAGCTGTG GTCATCCTGA GGTGGGCAGG CTAGGAGGGG GTCCTGCCTT GTACCCTCCT
    CCCGAAGGAC AGGTATGTAA CCCCCTGGAC TCTCTTGATC TTGACAACAC TCAGCTGGAC
    TTTGTGGCTA TTCTGGATGA GCCCCAGGGG CTGAGTCCTC CTCCTTCCCA TGATCAGCGG
    GGCAGCTCTG GACATACCCC ACCTCCCTCT GGGCCCCCCA ACATGGCTGT GGGCAACATG
    AGTGTCTTAC TGAGATCCCT ACCTGGGGAA ACAGAATTCC TCAACTCTAG TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153517.1
    CDS259..3195
    Translation

    Target ORF information:

    RefSeq Version NM_001160045.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGCCCAT CTCTGGGATT CCCAGCCCAG ATGAATCACC AAAAAGGGCC CTCGCCTTCC 
    TTTGGGGTCC AGCCTTGTGG TCCCCATGAC TCTGCCCGGG GTGGGATGAT CCCACATCCT
    CAGTCCCGGG GACCCTTCCC AACTTGCCAG CTGAAGTCTG AGCTGGACAT GCTGGTTGGC
    AAGTGCCGGG AGGAACCCTT GGAAGGTGAT ATGTCCAGCC CCAACTCCAC AGGCATACAG
    GATCCCCTGT TGGGGATGCT GGATGGGCGG GAGGACCTCG AGAGAGAGGA GAAGCGTGAG
    CCTGAATCTG TGTATGAAAC TGACTGCCGT TGGGATGGCT GCAGCCAGGA ATTTGACTCC
    CAAGAGCAGC TGGTGCACCA CATCAACAGC GAGCACATCC ACGGGGAGCG GAAGGAGTTC
    GTGTGCCACT GGGGGGGCTG CTCCAGGGAG CTGAGGCCCT TCAAAGCCCA GTACATGCTG
    GTGGTTCACA TGCGCAGACA CACTGGCGAG AAGCCACACA AGTGCACGTT TGAAGGGTGC
    CGGAAGTCAT ACTCACGCCT CGAAAACCTG AAGACGCACC TGCGGTCACA CACGGGTGAG
    AAGCCATACA TGTGTGAGCA CGAGGGCTGC AGTAAAGCCT TCAGCAATGC CAGTGACCGA
    GCCAAGCACC AGAATCGGAC CCATTCCAAT GAGAAGCCGT ATGTATGTAA GCTCCCTGGC
    TGCACCAAAC GCTATACAGA TCCTAGCTCG CTGCGAAAAC ATGTCAAGAC AGTGCATGGT
    CCTGACGCCC ATGTGACCAA ACGGCACCGT GGGGATGGCC CCCTGCCTCG GGCACCATCC
    ATTTCTACAG TGGAGCCCAA GAGGGAGCGG GAAGGAGGTC CCATCAGGGA GGAAAGCAGA
    CTGACTGTGC CAGAGGGTGC CATGAAGCCA CAGCCAAGCC CTGGGGCCCA GTCATCCTGC
    AGCAGTGACC ACTCCCCGGC AGGGAGTGCA GCCAATACAG ACAGTGGTGT GGAAATGACT
    GGCAATGCAG GGGGCAGCAC TGAAGACCTC TCCAGCTTGG ACGAGGGACC TTGCATTGCT
    GGCACTGGTC TGTCCACTCT TCGCCGCCTT GAGAACCTCA GGCTGGACCA GCTACATCAA
    CTCCGGCCAA TAGGGACCCG GGGTCTCAAA CTGCCCAGCT TGTCCCACAC CGGTACCACT
    GTGTCCCGCC GCGTGGGCCC CCCAGTCTCT CTTGAACGCC GCAGCAGCAG CTCCAGCAGC
    ATCAGCTCTG CCTATACTGT CAGCCGCCGC TCCTCCCTGG CCTCTCCTTT CCCCCCTGGC
    TCCCCACCAG AGAATGGAGC ATCCTCCCTG CCTGGCCTTA TGCCTGCCCA GCACTACCTG
    CTTCGGGCAA GATATGCTTC AGCCAGAGGG GGTGGTACTT CGCCCACTGC AGCATCCAGC
    CTGGATCGGA TAGGTGGTCT TCCCATGCCT CCTTGGAGAA GCCGAGCCGA GTATCCAGGA
    TACAACCCCA ATGCAGGGGT CACCCGGAGG GCCAGTGACC CAGCCCAGGC TGCTGACCGT
    CCTGCTCCAG CTAGAGTCCA GAGGTTCAAG AGCCTGGGCT GTGTCCATAC CCCACCCACT
    GTGGCAGGGG GAGGACAGAA CTTTGATCCT TACCTCCCAA CCTCTGTCTA CTCACCACAG
    CCCCCCAGCA TCACTGAGAA TGCTGCCATG GATGCTAGAG GGCTACAGGA AGAGCCAGAA
    GTTGGGACCT CCATGGTGGG CAGTGGTCTG AACCCCTATA TGGACTTCCC ACCTACTGAT
    ACTCTGGGAT ATGGGGGACC TGAAGGGGCA GCAGCTGAGC CTTATGGAGC GAGGGGTCCA
    GGCTCTCTGC CTCTTGGGCC TGGTCCACCC ACCAACTATG GCCCCAACCC CTGTCCCCAG
    CAGGCCTCAT ATCCTGACCC CACCCAAGAA ACATGGGGTG AGTTCCCTTC CCACTCTGGG
    CTGTACCCAG GCCCCAAGGC TCTAGGTGGA ACCTACAGCC AGTGTCCTCG ACTTGAACAT
    TATGGACAAG TGCAAGTCAA GCCAGAACAG GGGTGCCCAG TGGGGTCTGA CTCCACAGGA
    CTGGCACCCT GCCTCAATGC CCACCCCAGT GAGGGGCCCC CACATCCACA GCCTCTCTTT
    TCCCATTACC CCCAGCCCTC TCCTCCCCAA TATCTCCAGT CAGGCCCCTA TACCCAGCCA
    CCCCCTGATT ATCTTCCTTC AGAACCCAGG CCTTGCCTGG ACTTTGATTC CCCCACCCAT
    TCCACAGGGC AGCTCAAGGC TCAGCTTGTG TGTAATTATG TTCAATCTCA ACAGGAGCTA
    CTGTGGGAGG GTGGGGGCAG GGAAGATGCC CCCGCCCAGG AACCTTCCTA CCAGAGTCCC
    AAGTTTCTGG GGGGTTCCCA GGTTAGCCCA AGCCGTGCTA AAGCTCCAGT GAACACATAT
    GGACCTGGCT TTGGACCCAA CTTGCCCAAT CACAAGTCAG GTTCCTATCC CACCCCTTCA
    CCATGCCATG AAAATTTTGT AGTGGGGGCA AATAGGGCTT CACATAGGGC AGCAGCACCA
    CCTCGACTTC TGCCCCCATT GCCCACTTGC TATGGGCCTC TCAAAGTGGG AGGCACAAAC
    CCCAGCTGTG GTCATCCTGA GGTGGGCAGG CTAGGAGGGG GTCCTGCCTT GTACCCTCCT
    CCCGAAGGAC AGGTATGTAA CCCCCTGGAC TCTCTTGATC TTGACAACAC TCAGCTGGAC
    TTTGTGGCTA TTCTGGATGA GCCCCAGGGG CTGAGTCCTC CTCCTTCCCA TGATCAGCGG
    GGCAGCTCTG GACATACCCC ACCTCCCTCT GGGCCCCCCA ACATGGCTGT GGGCAACATG
    AGTGTCTTAC TGAGATCCCT ACCTGGGGAA ACAGAATTCC TCAACTCTAG TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22564
    Clone ID Related Accession (Same CDS sequence) XM_011538189.1 , XM_011538189.2 , NM_005269.2 , NM_005269.3
    Accession Version XM_011538189.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product zinc finger protein GLI1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCGTATGTAT GTAAGCTCCC TGGCTGCACC AAACGCTATA CAGATCCTAG CTCGCTGCGA
    AAACATGTCA AGACAGTGCA TGGTCCTGAC GCCCATGTGA CCAAACGGCA CCGTGGGGAT
    GGCCCCCTGC CTCGGGCACC ATCCATTTCT ACAGTGGAGC CCAAGAGGGA GCGGGAAGGA
    GGTCCCATCA GGGAGGAAAG CAGACTGACT GTGCCAGAGG GTGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CGGCAGGGAG TGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGCAAT GCAGGGGGCA GCACTGAAGA CCTCTCCAGC
    TTGGACGAGG GACCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAATAGGGA CCCGGGGTCT CAAACTGCCC
    AGCTTGTCCC ACACCGGTAC CACTGTGTCC CGCCGCGTGG GCCCCCCAGT CTCTCTTGAA
    CGCCGCAGCA GCAGCTCCAG CAGCATCAGC TCTGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCTC CTTTCCCCCC TGGCTCCCCA CCAGAGAATG GAGCATCCTC CCTGCCTGGC
    CTTATGCCTG CCCAGCACTA CCTGCTTCGG GCAAGATATG CTTCAGCCAG AGGGGGTGGT
    ACTTCGCCCA CTGCAGCATC CAGCCTGGAT CGGATAGGTG GTCTTCCCAT GCCTCCTTGG
    AGAAGCCGAG CCGAGTATCC AGGATACAAC CCCAATGCAG GGGTCACCCG GAGGGCCAGT
    GACCCAGCCC AGGCTGCTGA CCGTCCTGCT CCAGCTAGAG TCCAGAGGTT CAAGAGCCTG
    GGCTGTGTCC ATACCCCACC CACTGTGGCA GGGGGAGGAC AGAACTTTGA TCCTTACCTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGCTGC CATGGATGCT
    AGAGGGCTAC AGGAAGAGCC AGAAGTTGGG ACCTCCATGG TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTAC TGATACTCTG GGATATGGGG GACCTGAAGG GGCAGCAGCT
    GAGCCTTATG GAGCGAGGGG TCCAGGCTCT CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGCC TCATATCCTG ACCCCACCCA AGAAACATGG
    GGTGAGTTCC CTTCCCACTC TGGGCTGTAC CCAGGCCCCA AGGCTCTAGG TGGAACCTAC
    AGCCAGTGTC CTCGACTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGC
    CCAGTGGGGT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAGTGAGGGG
    CCCCCACATC CACAGCCTCT CTTTTCCCAT TACCCCCAGC CCTCTCCTCC CCAATATCTC
    CAGTCAGGCC CCTATACCCA GCCACCCCCT GATTATCTTC CTTCAGAACC CAGGCCTTGC
    CTGGACTTTG ATTCCCCCAC CCATTCCACA GGGCAGCTCA AGGCTCAGCT TGTGTGTAAT
    TATGTTCAAT CTCAACAGGA GCTACTGTGG GAGGGTGGGG GCAGGGAAGA TGCCCCCGCC
    CAGGAACCTT CCTACCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTTAG CCCAAGCCGT
    GCTAAAGCTC CAGTGAACAC ATATGGACCT GGCTTTGGAC CCAACTTGCC CAATCACAAG
    TCAGGTTCCT ATCCCACCCC TTCACCATGC CATGAAAATT TTGTAGTGGG GGCAAATAGG
    GCTTCACATA GGGCAGCAGC ACCACCTCGA CTTCTGCCCC CATTGCCCAC TTGCTATGGG
    CCTCTCAAAG TGGGAGGCAC AAACCCCAGC TGTGGTCATC CTGAGGTGGG CAGGCTAGGA
    GGGGGTCCTG CCTTGTACCC TCCTCCCGAA GGACAGGTAT GTAACCCCCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGCCCCA GGGGCTGAGT
    CCTCCTCCTT CCCATGATCA GCGGGGCAGC TCTGGACATA CCCCACCTCC CTCTGGGCCC
    CCCAACATGG CTGTGGGCAA CATGAGTGTC TTACTGAGAT CCCTACCTGG GGAAACAGAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536491.1
    Translation

    Target ORF information:

    RefSeq Version XM_011538189.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCGTATGTAT GTAAGCTCCC TGGCTGCACC AAACGCTATA CAGATCCTAG CTCGCTGCGA
    AAACATGTCA AGACAGTGCA TGGTCCTGAC GCCCATGTGA CCAAACGGCA CCGTGGGGAT
    GGCCCCCTGC CTCGGGCACC ATCCATTTCT ACAGTGGAGC CCAAGAGGGA GCGGGAAGGA
    GGTCCCATCA GGGAGGAAAG CAGACTGACT GTGCCAGAGG GTGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CGGCAGGGAG TGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGCAAT GCAGGGGGCA GCACTGAAGA CCTCTCCAGC
    TTGGACGAGG GACCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAATAGGGA CCCGGGGTCT CAAACTGCCC
    AGCTTGTCCC ACACCGGTAC CACTGTGTCC CGCCGCGTGG GCCCCCCAGT CTCTCTTGAA
    CGCCGCAGCA GCAGCTCCAG CAGCATCAGC TCTGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCTC CTTTCCCCCC TGGCTCCCCA CCAGAGAATG GAGCATCCTC CCTGCCTGGC
    CTTATGCCTG CCCAGCACTA CCTGCTTCGG GCAAGATATG CTTCAGCCAG AGGGGGTGGT
    ACTTCGCCCA CTGCAGCATC CAGCCTGGAT CGGATAGGTG GTCTTCCCAT GCCTCCTTGG
    AGAAGCCGAG CCGAGTATCC AGGATACAAC CCCAATGCAG GGGTCACCCG GAGGGCCAGT
    GACCCAGCCC AGGCTGCTGA CCGTCCTGCT CCAGCTAGAG TCCAGAGGTT CAAGAGCCTG
    GGCTGTGTCC ATACCCCACC CACTGTGGCA GGGGGAGGAC AGAACTTTGA TCCTTACCTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGCTGC CATGGATGCT
    AGAGGGCTAC AGGAAGAGCC AGAAGTTGGG ACCTCCATGG TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTAC TGATACTCTG GGATATGGGG GACCTGAAGG GGCAGCAGCT
    GAGCCTTATG GAGCGAGGGG TCCAGGCTCT CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGCC TCATATCCTG ACCCCACCCA AGAAACATGG
    GGTGAGTTCC CTTCCCACTC TGGGCTGTAC CCAGGCCCCA AGGCTCTAGG TGGAACCTAC
    AGCCAGTGTC CTCGACTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGC
    CCAGTGGGGT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAGTGAGGGG
    CCCCCACATC CACAGCCTCT CTTTTCCCAT TACCCCCAGC CCTCTCCTCC CCAATATCTC
    CAGTCAGGCC CCTATACCCA GCCACCCCCT GATTATCTTC CTTCAGAACC CAGGCCTTGC
    CTGGACTTTG ATTCCCCCAC CCATTCCACA GGGCAGCTCA AGGCTCAGCT TGTGTGTAAT
    TATGTTCAAT CTCAACAGGA GCTACTGTGG GAGGGTGGGG GCAGGGAAGA TGCCCCCGCC
    CAGGAACCTT CCTACCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTTAG CCCAAGCCGT
    GCTAAAGCTC CAGTGAACAC ATATGGACCT GGCTTTGGAC CCAACTTGCC CAATCACAAG
    TCAGGTTCCT ATCCCACCCC TTCACCATGC CATGAAAATT TTGTAGTGGG GGCAAATAGG
    GCTTCACATA GGGCAGCAGC ACCACCTCGA CTTCTGCCCC CATTGCCCAC TTGCTATGGG
    CCTCTCAAAG TGGGAGGCAC AAACCCCAGC TGTGGTCATC CTGAGGTGGG CAGGCTAGGA
    GGGGGTCCTG CCTTGTACCC TCCTCCCGAA GGACAGGTAT GTAACCCCCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGCCCCA GGGGCTGAGT
    CCTCCTCCTT CCCATGATCA GCGGGGCAGC TCTGGACATA CCCCACCTCC CTCTGGGCCC
    CCCAACATGG CTGTGGGCAA CATGAGTGTC TTACTGAGAT CCCTACCTGG GGAAACAGAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22564
    Clone ID Related Accession (Same CDS sequence) XM_011538189.1 , XM_011538189.2 , NM_005269.2 , NM_005269.3
    Accession Version XM_011538189.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538189.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCGTATGTAT GTAAGCTCCC TGGCTGCACC AAACGCTATA CAGATCCTAG CTCGCTGCGA
    AAACATGTCA AGACAGTGCA TGGTCCTGAC GCCCATGTGA CCAAACGGCA CCGTGGGGAT
    GGCCCCCTGC CTCGGGCACC ATCCATTTCT ACAGTGGAGC CCAAGAGGGA GCGGGAAGGA
    GGTCCCATCA GGGAGGAAAG CAGACTGACT GTGCCAGAGG GTGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CGGCAGGGAG TGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGCAAT GCAGGGGGCA GCACTGAAGA CCTCTCCAGC
    TTGGACGAGG GACCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAATAGGGA CCCGGGGTCT CAAACTGCCC
    AGCTTGTCCC ACACCGGTAC CACTGTGTCC CGCCGCGTGG GCCCCCCAGT CTCTCTTGAA
    CGCCGCAGCA GCAGCTCCAG CAGCATCAGC TCTGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCTC CTTTCCCCCC TGGCTCCCCA CCAGAGAATG GAGCATCCTC CCTGCCTGGC
    CTTATGCCTG CCCAGCACTA CCTGCTTCGG GCAAGATATG CTTCAGCCAG AGGGGGTGGT
    ACTTCGCCCA CTGCAGCATC CAGCCTGGAT CGGATAGGTG GTCTTCCCAT GCCTCCTTGG
    AGAAGCCGAG CCGAGTATCC AGGATACAAC CCCAATGCAG GGGTCACCCG GAGGGCCAGT
    GACCCAGCCC AGGCTGCTGA CCGTCCTGCT CCAGCTAGAG TCCAGAGGTT CAAGAGCCTG
    GGCTGTGTCC ATACCCCACC CACTGTGGCA GGGGGAGGAC AGAACTTTGA TCCTTACCTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGCTGC CATGGATGCT
    AGAGGGCTAC AGGAAGAGCC AGAAGTTGGG ACCTCCATGG TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTAC TGATACTCTG GGATATGGGG GACCTGAAGG GGCAGCAGCT
    GAGCCTTATG GAGCGAGGGG TCCAGGCTCT CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGCC TCATATCCTG ACCCCACCCA AGAAACATGG
    GGTGAGTTCC CTTCCCACTC TGGGCTGTAC CCAGGCCCCA AGGCTCTAGG TGGAACCTAC
    AGCCAGTGTC CTCGACTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGC
    CCAGTGGGGT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAGTGAGGGG
    CCCCCACATC CACAGCCTCT CTTTTCCCAT TACCCCCAGC CCTCTCCTCC CCAATATCTC
    CAGTCAGGCC CCTATACCCA GCCACCCCCT GATTATCTTC CTTCAGAACC CAGGCCTTGC
    CTGGACTTTG ATTCCCCCAC CCATTCCACA GGGCAGCTCA AGGCTCAGCT TGTGTGTAAT
    TATGTTCAAT CTCAACAGGA GCTACTGTGG GAGGGTGGGG GCAGGGAAGA TGCCCCCGCC
    CAGGAACCTT CCTACCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTTAG CCCAAGCCGT
    GCTAAAGCTC CAGTGAACAC ATATGGACCT GGCTTTGGAC CCAACTTGCC CAATCACAAG
    TCAGGTTCCT ATCCCACCCC TTCACCATGC CATGAAAATT TTGTAGTGGG GGCAAATAGG
    GCTTCACATA GGGCAGCAGC ACCACCTCGA CTTCTGCCCC CATTGCCCAC TTGCTATGGG
    CCTCTCAAAG TGGGAGGCAC AAACCCCAGC TGTGGTCATC CTGAGGTGGG CAGGCTAGGA
    GGGGGTCCTG CCTTGTACCC TCCTCCCGAA GGACAGGTAT GTAACCCCCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGCCCCA GGGGCTGAGT
    CCTCCTCCTT CCCATGATCA GCGGGGCAGC TCTGGACATA CCCCACCTCC CTCTGGGCCC
    CCCAACATGG CTGTGGGCAA CATGAGTGTC TTACTGAGAT CCCTACCTGG GGAAACAGAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536491.1
    CDS325..3645
    Translation

    Target ORF information:

    RefSeq Version XM_011538189.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538189.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCGTATGTAT GTAAGCTCCC TGGCTGCACC AAACGCTATA CAGATCCTAG CTCGCTGCGA
    AAACATGTCA AGACAGTGCA TGGTCCTGAC GCCCATGTGA CCAAACGGCA CCGTGGGGAT
    GGCCCCCTGC CTCGGGCACC ATCCATTTCT ACAGTGGAGC CCAAGAGGGA GCGGGAAGGA
    GGTCCCATCA GGGAGGAAAG CAGACTGACT GTGCCAGAGG GTGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CGGCAGGGAG TGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGCAAT GCAGGGGGCA GCACTGAAGA CCTCTCCAGC
    TTGGACGAGG GACCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAATAGGGA CCCGGGGTCT CAAACTGCCC
    AGCTTGTCCC ACACCGGTAC CACTGTGTCC CGCCGCGTGG GCCCCCCAGT CTCTCTTGAA
    CGCCGCAGCA GCAGCTCCAG CAGCATCAGC TCTGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCTC CTTTCCCCCC TGGCTCCCCA CCAGAGAATG GAGCATCCTC CCTGCCTGGC
    CTTATGCCTG CCCAGCACTA CCTGCTTCGG GCAAGATATG CTTCAGCCAG AGGGGGTGGT
    ACTTCGCCCA CTGCAGCATC CAGCCTGGAT CGGATAGGTG GTCTTCCCAT GCCTCCTTGG
    AGAAGCCGAG CCGAGTATCC AGGATACAAC CCCAATGCAG GGGTCACCCG GAGGGCCAGT
    GACCCAGCCC AGGCTGCTGA CCGTCCTGCT CCAGCTAGAG TCCAGAGGTT CAAGAGCCTG
    GGCTGTGTCC ATACCCCACC CACTGTGGCA GGGGGAGGAC AGAACTTTGA TCCTTACCTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGCTGC CATGGATGCT
    AGAGGGCTAC AGGAAGAGCC AGAAGTTGGG ACCTCCATGG TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTAC TGATACTCTG GGATATGGGG GACCTGAAGG GGCAGCAGCT
    GAGCCTTATG GAGCGAGGGG TCCAGGCTCT CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGCC TCATATCCTG ACCCCACCCA AGAAACATGG
    GGTGAGTTCC CTTCCCACTC TGGGCTGTAC CCAGGCCCCA AGGCTCTAGG TGGAACCTAC
    AGCCAGTGTC CTCGACTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGC
    CCAGTGGGGT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAGTGAGGGG
    CCCCCACATC CACAGCCTCT CTTTTCCCAT TACCCCCAGC CCTCTCCTCC CCAATATCTC
    CAGTCAGGCC CCTATACCCA GCCACCCCCT GATTATCTTC CTTCAGAACC CAGGCCTTGC
    CTGGACTTTG ATTCCCCCAC CCATTCCACA GGGCAGCTCA AGGCTCAGCT TGTGTGTAAT
    TATGTTCAAT CTCAACAGGA GCTACTGTGG GAGGGTGGGG GCAGGGAAGA TGCCCCCGCC
    CAGGAACCTT CCTACCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTTAG CCCAAGCCGT
    GCTAAAGCTC CAGTGAACAC ATATGGACCT GGCTTTGGAC CCAACTTGCC CAATCACAAG
    TCAGGTTCCT ATCCCACCCC TTCACCATGC CATGAAAATT TTGTAGTGGG GGCAAATAGG
    GCTTCACATA GGGCAGCAGC ACCACCTCGA CTTCTGCCCC CATTGCCCAC TTGCTATGGG
    CCTCTCAAAG TGGGAGGCAC AAACCCCAGC TGTGGTCATC CTGAGGTGGG CAGGCTAGGA
    GGGGGTCCTG CCTTGTACCC TCCTCCCGAA GGACAGGTAT GTAACCCCCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGCCCCA GGGGCTGAGT
    CCTCCTCCTT CCCATGATCA GCGGGGCAGC TCTGGACATA CCCCACCTCC CTCTGGGCCC
    CCCAACATGG CTGTGGGCAA CATGAGTGTC TTACTGAGAT CCCTACCTGG GGAAACAGAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23011
    Clone ID Related Accession (Same CDS sequence) NM_001160045.1 , XM_005268799.2
    Accession Version XM_005268799.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product zinc finger protein GLI1 isoform X2
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGCCCAT CTCTGGGATT CCCAGCCCAG ATGAATCACC AAAAAGGGCC CTCGCCTTCC 
    TTTGGGGTCC AGCCTTGTGG TCCCCATGAC TCTGCCCGGG GTGGGATGAT CCCACATCCT
    CAGTCCCGGG GACCCTTCCC AACTTGCCAG CTGAAGTCTG AGCTGGACAT GCTGGTTGGC
    AAGTGCCGGG AGGAACCCTT GGAAGGTGAT ATGTCCAGCC CCAACTCCAC AGGCATACAG
    GATCCCCTGT TGGGGATGCT GGATGGGCGG GAGGACCTCG AGAGAGAGGA GAAGCGTGAG
    CCTGAATCTG TGTATGAAAC TGACTGCCGT TGGGATGGCT GCAGCCAGGA ATTTGACTCC
    CAAGAGCAGC TGGTGCACCA CATCAACAGC GAGCACATCC ACGGGGAGCG GAAGGAGTTC
    GTGTGCCACT GGGGGGGCTG CTCCAGGGAG CTGAGGCCCT TCAAAGCCCA GTACATGCTG
    GTGGTTCACA TGCGCAGACA CACTGGCGAG AAGCCACACA AGTGCACGTT TGAAGGGTGC
    CGGAAGTCAT ACTCACGCCT CGAAAACCTG AAGACGCACC TGCGGTCACA CACGGGTGAG
    AAGCCATACA TGTGTGAGCA CGAGGGCTGC AGTAAAGCCT TCAGCAATGC CAGTGACCGA
    GCCAAGCACC AGAATCGGAC CCATTCCAAT GAGAAGCCGT ATGTATGTAA GCTCCCTGGC
    TGCACCAAAC GCTATACAGA TCCTAGCTCG CTGCGAAAAC ATGTCAAGAC AGTGCATGGT
    CCTGACGCCC ATGTGACCAA ACGGCACCGT GGGGATGGCC CCCTGCCTCG GGCACCATCC
    ATTTCTACAG TGGAGCCCAA GAGGGAGCGG GAAGGAGGTC CCATCAGGGA GGAAAGCAGA
    CTGACTGTGC CAGAGGGTGC CATGAAGCCA CAGCCAAGCC CTGGGGCCCA GTCATCCTGC
    AGCAGTGACC ACTCCCCGGC AGGGAGTGCA GCCAATACAG ACAGTGGTGT GGAAATGACT
    GGCAATGCAG GGGGCAGCAC TGAAGACCTC TCCAGCTTGG ACGAGGGACC TTGCATTGCT
    GGCACTGGTC TGTCCACTCT TCGCCGCCTT GAGAACCTCA GGCTGGACCA GCTACATCAA
    CTCCGGCCAA TAGGGACCCG GGGTCTCAAA CTGCCCAGCT TGTCCCACAC CGGTACCACT
    GTGTCCCGCC GCGTGGGCCC CCCAGTCTCT CTTGAACGCC GCAGCAGCAG CTCCAGCAGC
    ATCAGCTCTG CCTATACTGT CAGCCGCCGC TCCTCCCTGG CCTCTCCTTT CCCCCCTGGC
    TCCCCACCAG AGAATGGAGC ATCCTCCCTG CCTGGCCTTA TGCCTGCCCA GCACTACCTG
    CTTCGGGCAA GATATGCTTC AGCCAGAGGG GGTGGTACTT CGCCCACTGC AGCATCCAGC
    CTGGATCGGA TAGGTGGTCT TCCCATGCCT CCTTGGAGAA GCCGAGCCGA GTATCCAGGA
    TACAACCCCA ATGCAGGGGT CACCCGGAGG GCCAGTGACC CAGCCCAGGC TGCTGACCGT
    CCTGCTCCAG CTAGAGTCCA GAGGTTCAAG AGCCTGGGCT GTGTCCATAC CCCACCCACT
    GTGGCAGGGG GAGGACAGAA CTTTGATCCT TACCTCCCAA CCTCTGTCTA CTCACCACAG
    CCCCCCAGCA TCACTGAGAA TGCTGCCATG GATGCTAGAG GGCTACAGGA AGAGCCAGAA
    GTTGGGACCT CCATGGTGGG CAGTGGTCTG AACCCCTATA TGGACTTCCC ACCTACTGAT
    ACTCTGGGAT ATGGGGGACC TGAAGGGGCA GCAGCTGAGC CTTATGGAGC GAGGGGTCCA
    GGCTCTCTGC CTCTTGGGCC TGGTCCACCC ACCAACTATG GCCCCAACCC CTGTCCCCAG
    CAGGCCTCAT ATCCTGACCC CACCCAAGAA ACATGGGGTG AGTTCCCTTC CCACTCTGGG
    CTGTACCCAG GCCCCAAGGC TCTAGGTGGA ACCTACAGCC AGTGTCCTCG ACTTGAACAT
    TATGGACAAG TGCAAGTCAA GCCAGAACAG GGGTGCCCAG TGGGGTCTGA CTCCACAGGA
    CTGGCACCCT GCCTCAATGC CCACCCCAGT GAGGGGCCCC CACATCCACA GCCTCTCTTT
    TCCCATTACC CCCAGCCCTC TCCTCCCCAA TATCTCCAGT CAGGCCCCTA TACCCAGCCA
    CCCCCTGATT ATCTTCCTTC AGAACCCAGG CCTTGCCTGG ACTTTGATTC CCCCACCCAT
    TCCACAGGGC AGCTCAAGGC TCAGCTTGTG TGTAATTATG TTCAATCTCA ACAGGAGCTA
    CTGTGGGAGG GTGGGGGCAG GGAAGATGCC CCCGCCCAGG AACCTTCCTA CCAGAGTCCC
    AAGTTTCTGG GGGGTTCCCA GGTTAGCCCA AGCCGTGCTA AAGCTCCAGT GAACACATAT
    GGACCTGGCT TTGGACCCAA CTTGCCCAAT CACAAGTCAG GTTCCTATCC CACCCCTTCA
    CCATGCCATG AAAATTTTGT AGTGGGGGCA AATAGGGCTT CACATAGGGC AGCAGCACCA
    CCTCGACTTC TGCCCCCATT GCCCACTTGC TATGGGCCTC TCAAAGTGGG AGGCACAAAC
    CCCAGCTGTG GTCATCCTGA GGTGGGCAGG CTAGGAGGGG GTCCTGCCTT GTACCCTCCT
    CCCGAAGGAC AGGTATGTAA CCCCCTGGAC TCTCTTGATC TTGACAACAC TCAGCTGGAC
    TTTGTGGCTA TTCTGGATGA GCCCCAGGGG CTGAGTCCTC CTCCTTCCCA TGATCAGCGG
    GGCAGCTCTG GACATACCCC ACCTCCCTCT GGGCCCCCCA ACATGGCTGT GGGCAACATG
    AGTGTCTTAC TGAGATCCCT ACCTGGGGAA ACAGAATTCC TCAACTCTAG TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005268856.1
    Translation

    Target ORF information:

    RefSeq Version XM_005268799.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005268799.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGCCCAT CTCTGGGATT CCCAGCCCAG ATGAATCACC AAAAAGGGCC CTCGCCTTCC 
    TTTGGGGTCC AGCCTTGTGG TCCCCATGAC TCTGCCCGGG GTGGGATGAT CCCACATCCT
    CAGTCCCGGG GACCCTTCCC AACTTGCCAG CTGAAGTCTG AGCTGGACAT GCTGGTTGGC
    AAGTGCCGGG AGGAACCCTT GGAAGGTGAT ATGTCCAGCC CCAACTCCAC AGGCATACAG
    GATCCCCTGT TGGGGATGCT GGATGGGCGG GAGGACCTCG AGAGAGAGGA GAAGCGTGAG
    CCTGAATCTG TGTATGAAAC TGACTGCCGT TGGGATGGCT GCAGCCAGGA ATTTGACTCC
    CAAGAGCAGC TGGTGCACCA CATCAACAGC GAGCACATCC ACGGGGAGCG GAAGGAGTTC
    GTGTGCCACT GGGGGGGCTG CTCCAGGGAG CTGAGGCCCT TCAAAGCCCA GTACATGCTG
    GTGGTTCACA TGCGCAGACA CACTGGCGAG AAGCCACACA AGTGCACGTT TGAAGGGTGC
    CGGAAGTCAT ACTCACGCCT CGAAAACCTG AAGACGCACC TGCGGTCACA CACGGGTGAG
    AAGCCATACA TGTGTGAGCA CGAGGGCTGC AGTAAAGCCT TCAGCAATGC CAGTGACCGA
    GCCAAGCACC AGAATCGGAC CCATTCCAAT GAGAAGCCGT ATGTATGTAA GCTCCCTGGC
    TGCACCAAAC GCTATACAGA TCCTAGCTCG CTGCGAAAAC ATGTCAAGAC AGTGCATGGT
    CCTGACGCCC ATGTGACCAA ACGGCACCGT GGGGATGGCC CCCTGCCTCG GGCACCATCC
    ATTTCTACAG TGGAGCCCAA GAGGGAGCGG GAAGGAGGTC CCATCAGGGA GGAAAGCAGA
    CTGACTGTGC CAGAGGGTGC CATGAAGCCA CAGCCAAGCC CTGGGGCCCA GTCATCCTGC
    AGCAGTGACC ACTCCCCGGC AGGGAGTGCA GCCAATACAG ACAGTGGTGT GGAAATGACT
    GGCAATGCAG GGGGCAGCAC TGAAGACCTC TCCAGCTTGG ACGAGGGACC TTGCATTGCT
    GGCACTGGTC TGTCCACTCT TCGCCGCCTT GAGAACCTCA GGCTGGACCA GCTACATCAA
    CTCCGGCCAA TAGGGACCCG GGGTCTCAAA CTGCCCAGCT TGTCCCACAC CGGTACCACT
    GTGTCCCGCC GCGTGGGCCC CCCAGTCTCT CTTGAACGCC GCAGCAGCAG CTCCAGCAGC
    ATCAGCTCTG CCTATACTGT CAGCCGCCGC TCCTCCCTGG CCTCTCCTTT CCCCCCTGGC
    TCCCCACCAG AGAATGGAGC ATCCTCCCTG CCTGGCCTTA TGCCTGCCCA GCACTACCTG
    CTTCGGGCAA GATATGCTTC AGCCAGAGGG GGTGGTACTT CGCCCACTGC AGCATCCAGC
    CTGGATCGGA TAGGTGGTCT TCCCATGCCT CCTTGGAGAA GCCGAGCCGA GTATCCAGGA
    TACAACCCCA ATGCAGGGGT CACCCGGAGG GCCAGTGACC CAGCCCAGGC TGCTGACCGT
    CCTGCTCCAG CTAGAGTCCA GAGGTTCAAG AGCCTGGGCT GTGTCCATAC CCCACCCACT
    GTGGCAGGGG GAGGACAGAA CTTTGATCCT TACCTCCCAA CCTCTGTCTA CTCACCACAG
    CCCCCCAGCA TCACTGAGAA TGCTGCCATG GATGCTAGAG GGCTACAGGA AGAGCCAGAA
    GTTGGGACCT CCATGGTGGG CAGTGGTCTG AACCCCTATA TGGACTTCCC ACCTACTGAT
    ACTCTGGGAT ATGGGGGACC TGAAGGGGCA GCAGCTGAGC CTTATGGAGC GAGGGGTCCA
    GGCTCTCTGC CTCTTGGGCC TGGTCCACCC ACCAACTATG GCCCCAACCC CTGTCCCCAG
    CAGGCCTCAT ATCCTGACCC CACCCAAGAA ACATGGGGTG AGTTCCCTTC CCACTCTGGG
    CTGTACCCAG GCCCCAAGGC TCTAGGTGGA ACCTACAGCC AGTGTCCTCG ACTTGAACAT
    TATGGACAAG TGCAAGTCAA GCCAGAACAG GGGTGCCCAG TGGGGTCTGA CTCCACAGGA
    CTGGCACCCT GCCTCAATGC CCACCCCAGT GAGGGGCCCC CACATCCACA GCCTCTCTTT
    TCCCATTACC CCCAGCCCTC TCCTCCCCAA TATCTCCAGT CAGGCCCCTA TACCCAGCCA
    CCCCCTGATT ATCTTCCTTC AGAACCCAGG CCTTGCCTGG ACTTTGATTC CCCCACCCAT
    TCCACAGGGC AGCTCAAGGC TCAGCTTGTG TGTAATTATG TTCAATCTCA ACAGGAGCTA
    CTGTGGGAGG GTGGGGGCAG GGAAGATGCC CCCGCCCAGG AACCTTCCTA CCAGAGTCCC
    AAGTTTCTGG GGGGTTCCCA GGTTAGCCCA AGCCGTGCTA AAGCTCCAGT GAACACATAT
    GGACCTGGCT TTGGACCCAA CTTGCCCAAT CACAAGTCAG GTTCCTATCC CACCCCTTCA
    CCATGCCATG AAAATTTTGT AGTGGGGGCA AATAGGGCTT CACATAGGGC AGCAGCACCA
    CCTCGACTTC TGCCCCCATT GCCCACTTGC TATGGGCCTC TCAAAGTGGG AGGCACAAAC
    CCCAGCTGTG GTCATCCTGA GGTGGGCAGG CTAGGAGGGG GTCCTGCCTT GTACCCTCCT
    CCCGAAGGAC AGGTATGTAA CCCCCTGGAC TCTCTTGATC TTGACAACAC TCAGCTGGAC
    TTTGTGGCTA TTCTGGATGA GCCCCAGGGG CTGAGTCCTC CTCCTTCCCA TGATCAGCGG
    GGCAGCTCTG GACATACCCC ACCTCCCTCT GGGCCCCCCA ACATGGCTGT GGGCAACATG
    AGTGTCTTAC TGAGATCCCT ACCTGGGGAA ACAGAATTCC TCAACTCTAG TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20556
    Clone ID Related Accession (Same CDS sequence) NM_001167609.1
    Accession Version NM_001167609.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product zinc finger protein GLI1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022506.38. Transcript Variant: This variant (3) lacks an in-frame segment in the 5' coding region, compared to variant 1, resulting in a shorter protein isoform (3), also known as tGLI1. This variant reflects the transcript and protein described by Lo (PubMed ID 19706761). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG TCAAGTTGAC CAAGAAGCGG
    GCACTGTCCA TCTCACCTCT GTCGGATGCC AGCCTGGACC TGCAGACGGT TATCCGCACC
    TCACCCAGCT CCCTCGTAGC TTTCATCAAC TCGCGATGCA CATCTCCAGG AGGCTCCTAC
    GGTCATCTCT CCATTGGCAC CATGAGCCCA TCTCTGGGAT TCCCAGCCCA GATGAATCAC
    CAAAAAGGGC CCTCGCCTTC CTTTGGGGTC CAGCCTTGTG GTCCCCATGA CTCTGCCCGG
    GGTGGGATGA TCCCACATCC TCAGTCCCGG GGACCCTTCC CAACTTGCCA GCTGAAGTCT
    GAGCTGGACA TGCTGGTTGG CAAGTGCCGG GAGGAACCCT TGGAAGGTGA TATGTCCAGC
    CCCAACTCCA CAGGCATACA GGATCCCCTG TTGGGGATGC TGGATGGGCG GGAGGACCTC
    GAGAGAGAGG AGAAGCGTGA GCCTGAATCT GTGTATGAAA CTGACTGCCG TTGGGATGGC
    TGCAGCCAGG AATTTGACTC CCAAGAGCAG CTGGTGCACC ACATCAACAG CGAGCACATC
    CACGGGGAGC GGAAGGAGTT CGTGTGCCAC TGGGGGGGCT GCTCCAGGGA GCTGAGGCCC
    TTCAAAGCCC AGTACATGCT GGTGGTTCAC ATGCGCAGAC ACACTGGCGA GAAGCCACAC
    AAGTGCACGT TTGAAGGGTG CCGGAAGTCA TACTCACGCC TCGAAAACCT GAAGACGCAC
    CTGCGGTCAC ACACGGGTGA GAAGCCATAC ATGTGTGAGC ACGAGGGCTG CAGTAAAGCC
    TTCAGCAATG CCAGTGACCG AGCCAAGCAC CAGAATCGGA CCCATTCCAA TGAGAAGCCG
    TATGTATGTA AGCTCCCTGG CTGCACCAAA CGCTATACAG ATCCTAGCTC GCTGCGAAAA
    CATGTCAAGA CAGTGCATGG TCCTGACGCC CATGTGACCA AACGGCACCG TGGGGATGGC
    CCCCTGCCTC GGGCACCATC CATTTCTACA GTGGAGCCCA AGAGGGAGCG GGAAGGAGGT
    CCCATCAGGG AGGAAAGCAG ACTGACTGTG CCAGAGGGTG CCATGAAGCC ACAGCCAAGC
    CCTGGGGCCC AGTCATCCTG CAGCAGTGAC CACTCCCCGG CAGGGAGTGC AGCCAATACA
    GACAGTGGTG TGGAAATGAC TGGCAATGCA GGGGGCAGCA CTGAAGACCT CTCCAGCTTG
    GACGAGGGAC CTTGCATTGC TGGCACTGGT CTGTCCACTC TTCGCCGCCT TGAGAACCTC
    AGGCTGGACC AGCTACATCA ACTCCGGCCA ATAGGGACCC GGGGTCTCAA ACTGCCCAGC
    TTGTCCCACA CCGGTACCAC TGTGTCCCGC CGCGTGGGCC CCCCAGTCTC TCTTGAACGC
    CGCAGCAGCA GCTCCAGCAG CATCAGCTCT GCCTATACTG TCAGCCGCCG CTCCTCCCTG
    GCCTCTCCTT TCCCCCCTGG CTCCCCACCA GAGAATGGAG CATCCTCCCT GCCTGGCCTT
    ATGCCTGCCC AGCACTACCT GCTTCGGGCA AGATATGCTT CAGCCAGAGG GGGTGGTACT
    TCGCCCACTG CAGCATCCAG CCTGGATCGG ATAGGTGGTC TTCCCATGCC TCCTTGGAGA
    AGCCGAGCCG AGTATCCAGG ATACAACCCC AATGCAGGGG TCACCCGGAG GGCCAGTGAC
    CCAGCCCAGG CTGCTGACCG TCCTGCTCCA GCTAGAGTCC AGAGGTTCAA GAGCCTGGGC
    TGTGTCCATA CCCCACCCAC TGTGGCAGGG GGAGGACAGA ACTTTGATCC TTACCTCCCA
    ACCTCTGTCT ACTCACCACA GCCCCCCAGC ATCACTGAGA ATGCTGCCAT GGATGCTAGA
    GGGCTACAGG AAGAGCCAGA AGTTGGGACC TCCATGGTGG GCAGTGGTCT GAACCCCTAT
    ATGGACTTCC CACCTACTGA TACTCTGGGA TATGGGGGAC CTGAAGGGGC AGCAGCTGAG
    CCTTATGGAG CGAGGGGTCC AGGCTCTCTG CCTCTTGGGC CTGGTCCACC CACCAACTAT
    GGCCCCAACC CCTGTCCCCA GCAGGCCTCA TATCCTGACC CCACCCAAGA AACATGGGGT
    GAGTTCCCTT CCCACTCTGG GCTGTACCCA GGCCCCAAGG CTCTAGGTGG AACCTACAGC
    CAGTGTCCTC GACTTGAACA TTATGGACAA GTGCAAGTCA AGCCAGAACA GGGGTGCCCA
    GTGGGGTCTG ACTCCACAGG ACTGGCACCC TGCCTCAATG CCCACCCCAG TGAGGGGCCC
    CCACATCCAC AGCCTCTCTT TTCCCATTAC CCCCAGCCCT CTCCTCCCCA ATATCTCCAG
    TCAGGCCCCT ATACCCAGCC ACCCCCTGAT TATCTTCCTT CAGAACCCAG GCCTTGCCTG
    GACTTTGATT CCCCCACCCA TTCCACAGGG CAGCTCAAGG CTCAGCTTGT GTGTAATTAT
    GTTCAATCTC AACAGGAGCT ACTGTGGGAG GGTGGGGGCA GGGAAGATGC CCCCGCCCAG
    GAACCTTCCT ACCAGAGTCC CAAGTTTCTG GGGGGTTCCC AGGTTAGCCC AAGCCGTGCT
    AAAGCTCCAG TGAACACATA TGGACCTGGC TTTGGACCCA ACTTGCCCAA TCACAAGTCA
    GGTTCCTATC CCACCCCTTC ACCATGCCAT GAAAATTTTG TAGTGGGGGC AAATAGGGCT
    TCACATAGGG CAGCAGCACC ACCTCGACTT CTGCCCCCAT TGCCCACTTG CTATGGGCCT
    CTCAAAGTGG GAGGCACAAA CCCCAGCTGT GGTCATCCTG AGGTGGGCAG GCTAGGAGGG
    GGTCCTGCCT TGTACCCTCC TCCCGAAGGA CAGGTATGTA ACCCCCTGGA CTCTCTTGAT
    CTTGACAACA CTCAGCTGGA CTTTGTGGCT ATTCTGGATG AGCCCCAGGG GCTGAGTCCT
    CCTCCTTCCC ATGATCAGCG GGGCAGCTCT GGACATACCC CACCTCCCTC TGGGCCCCCC
    AACATGGCTG TGGGCAACAT GAGTGTCTTA CTGAGATCCC TACCTGGGGA AACAGAATTC
    CTCAACTCTA GTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001161081.1
    CDS79..3276
    Translation

    Target ORF information:

    RefSeq Version NM_001167609.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001167609.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG TCAAGTTGAC CAAGAAGCGG
    GCACTGTCCA TCTCACCTCT GTCGGATGCC AGCCTGGACC TGCAGACGGT TATCCGCACC
    TCACCCAGCT CCCTCGTAGC TTTCATCAAC TCGCGATGCA CATCTCCAGG AGGCTCCTAC
    GGTCATCTCT CCATTGGCAC CATGAGCCCA TCTCTGGGAT TCCCAGCCCA GATGAATCAC
    CAAAAAGGGC CCTCGCCTTC CTTTGGGGTC CAGCCTTGTG GTCCCCATGA CTCTGCCCGG
    GGTGGGATGA TCCCACATCC TCAGTCCCGG GGACCCTTCC CAACTTGCCA GCTGAAGTCT
    GAGCTGGACA TGCTGGTTGG CAAGTGCCGG GAGGAACCCT TGGAAGGTGA TATGTCCAGC
    CCCAACTCCA CAGGCATACA GGATCCCCTG TTGGGGATGC TGGATGGGCG GGAGGACCTC
    GAGAGAGAGG AGAAGCGTGA GCCTGAATCT GTGTATGAAA CTGACTGCCG TTGGGATGGC
    TGCAGCCAGG AATTTGACTC CCAAGAGCAG CTGGTGCACC ACATCAACAG CGAGCACATC
    CACGGGGAGC GGAAGGAGTT CGTGTGCCAC TGGGGGGGCT GCTCCAGGGA GCTGAGGCCC
    TTCAAAGCCC AGTACATGCT GGTGGTTCAC ATGCGCAGAC ACACTGGCGA GAAGCCACAC
    AAGTGCACGT TTGAAGGGTG CCGGAAGTCA TACTCACGCC TCGAAAACCT GAAGACGCAC
    CTGCGGTCAC ACACGGGTGA GAAGCCATAC ATGTGTGAGC ACGAGGGCTG CAGTAAAGCC
    TTCAGCAATG CCAGTGACCG AGCCAAGCAC CAGAATCGGA CCCATTCCAA TGAGAAGCCG
    TATGTATGTA AGCTCCCTGG CTGCACCAAA CGCTATACAG ATCCTAGCTC GCTGCGAAAA
    CATGTCAAGA CAGTGCATGG TCCTGACGCC CATGTGACCA AACGGCACCG TGGGGATGGC
    CCCCTGCCTC GGGCACCATC CATTTCTACA GTGGAGCCCA AGAGGGAGCG GGAAGGAGGT
    CCCATCAGGG AGGAAAGCAG ACTGACTGTG CCAGAGGGTG CCATGAAGCC ACAGCCAAGC
    CCTGGGGCCC AGTCATCCTG CAGCAGTGAC CACTCCCCGG CAGGGAGTGC AGCCAATACA
    GACAGTGGTG TGGAAATGAC TGGCAATGCA GGGGGCAGCA CTGAAGACCT CTCCAGCTTG
    GACGAGGGAC CTTGCATTGC TGGCACTGGT CTGTCCACTC TTCGCCGCCT TGAGAACCTC
    AGGCTGGACC AGCTACATCA ACTCCGGCCA ATAGGGACCC GGGGTCTCAA ACTGCCCAGC
    TTGTCCCACA CCGGTACCAC TGTGTCCCGC CGCGTGGGCC CCCCAGTCTC TCTTGAACGC
    CGCAGCAGCA GCTCCAGCAG CATCAGCTCT GCCTATACTG TCAGCCGCCG CTCCTCCCTG
    GCCTCTCCTT TCCCCCCTGG CTCCCCACCA GAGAATGGAG CATCCTCCCT GCCTGGCCTT
    ATGCCTGCCC AGCACTACCT GCTTCGGGCA AGATATGCTT CAGCCAGAGG GGGTGGTACT
    TCGCCCACTG CAGCATCCAG CCTGGATCGG ATAGGTGGTC TTCCCATGCC TCCTTGGAGA
    AGCCGAGCCG AGTATCCAGG ATACAACCCC AATGCAGGGG TCACCCGGAG GGCCAGTGAC
    CCAGCCCAGG CTGCTGACCG TCCTGCTCCA GCTAGAGTCC AGAGGTTCAA GAGCCTGGGC
    TGTGTCCATA CCCCACCCAC TGTGGCAGGG GGAGGACAGA ACTTTGATCC TTACCTCCCA
    ACCTCTGTCT ACTCACCACA GCCCCCCAGC ATCACTGAGA ATGCTGCCAT GGATGCTAGA
    GGGCTACAGG AAGAGCCAGA AGTTGGGACC TCCATGGTGG GCAGTGGTCT GAACCCCTAT
    ATGGACTTCC CACCTACTGA TACTCTGGGA TATGGGGGAC CTGAAGGGGC AGCAGCTGAG
    CCTTATGGAG CGAGGGGTCC AGGCTCTCTG CCTCTTGGGC CTGGTCCACC CACCAACTAT
    GGCCCCAACC CCTGTCCCCA GCAGGCCTCA TATCCTGACC CCACCCAAGA AACATGGGGT
    GAGTTCCCTT CCCACTCTGG GCTGTACCCA GGCCCCAAGG CTCTAGGTGG AACCTACAGC
    CAGTGTCCTC GACTTGAACA TTATGGACAA GTGCAAGTCA AGCCAGAACA GGGGTGCCCA
    GTGGGGTCTG ACTCCACAGG ACTGGCACCC TGCCTCAATG CCCACCCCAG TGAGGGGCCC
    CCACATCCAC AGCCTCTCTT TTCCCATTAC CCCCAGCCCT CTCCTCCCCA ATATCTCCAG
    TCAGGCCCCT ATACCCAGCC ACCCCCTGAT TATCTTCCTT CAGAACCCAG GCCTTGCCTG
    GACTTTGATT CCCCCACCCA TTCCACAGGG CAGCTCAAGG CTCAGCTTGT GTGTAATTAT
    GTTCAATCTC AACAGGAGCT ACTGTGGGAG GGTGGGGGCA GGGAAGATGC CCCCGCCCAG
    GAACCTTCCT ACCAGAGTCC CAAGTTTCTG GGGGGTTCCC AGGTTAGCCC AAGCCGTGCT
    AAAGCTCCAG TGAACACATA TGGACCTGGC TTTGGACCCA ACTTGCCCAA TCACAAGTCA
    GGTTCCTATC CCACCCCTTC ACCATGCCAT GAAAATTTTG TAGTGGGGGC AAATAGGGCT
    TCACATAGGG CAGCAGCACC ACCTCGACTT CTGCCCCCAT TGCCCACTTG CTATGGGCCT
    CTCAAAGTGG GAGGCACAAA CCCCAGCTGT GGTCATCCTG AGGTGGGCAG GCTAGGAGGG
    GGTCCTGCCT TGTACCCTCC TCCCGAAGGA CAGGTATGTA ACCCCCTGGA CTCTCTTGAT
    CTTGACAACA CTCAGCTGGA CTTTGTGGCT ATTCTGGATG AGCCCCAGGG GCTGAGTCCT
    CCTCCTTCCC ATGATCAGCG GGGCAGCTCT GGACATACCC CACCTCCCTC TGGGCCCCCC
    AACATGGCTG TGGGCAACAT GAGTGTCTTA CTGAGATCCC TACCTGGGGA AACAGAATTC
    CTCAACTCTA GTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57981
    Clone ID Related Accession (Same CDS sequence) XM_011538190.1 , XM_011538190.2
    Accession Version XM_011538190.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product zinc finger protein GLI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCACAGCCAA GCCCTGGGGC CCAGTCATCC TGCAGCAGTG ACCACTCCCC GGCAGGGAGT
    GCAGCCAATA CAGACAGTGG TGTGGAAATG ACTGGCAATG CAGGGGGCAG CACTGAAGAC
    CTCTCCAGCT TGGACGAGGG ACCTTGCATT GCTGGCACTG GTCTGTCCAC TCTTCGCCGC
    CTTGAGAACC TCAGGCTGGA CCAGCTACAT CAACTCCGGC CAATAGGGAC CCGGGGTCTC
    AAACTGCCCA GCTTGTCCCA CACCGGTACC ACTGTGTCCC GCCGCGTGGG CCCCCCAGTC
    TCTCTTGAAC GCCGCAGCAG CAGCTCCAGC AGCATCAGCT CTGCCTATAC TGTCAGCCGC
    CGCTCCTCCC TGGCCTCTCC TTTCCCCCCT GGCTCCCCAC CAGAGAATGG AGCATCCTCC
    CTGCCTGGCC TTATGCCTGC CCAGCACTAC CTGCTTCGGG CAAGATATGC TTCAGCCAGA
    GGGGGTGGTA CTTCGCCCAC TGCAGCATCC AGCCTGGATC GGATAGGTGG TCTTCCCATG
    CCTCCTTGGA GAAGCCGAGC CGAGTATCCA GGATACAACC CCAATGCAGG GGTCACCCGG
    AGGGCCAGTG ACCCAGCCCA GGCTGCTGAC CGTCCTGCTC CAGCTAGAGT CCAGAGGTTC
    AAGAGCCTGG GCTGTGTCCA TACCCCACCC ACTGTGGCAG GGGGAGGACA GAACTTTGAT
    CCTTACCTCC CAACCTCTGT CTACTCACCA CAGCCCCCCA GCATCACTGA GAATGCTGCC
    ATGGATGCTA GAGGGCTACA GGAAGAGCCA GAAGTTGGGA CCTCCATGGT GGGCAGTGGT
    CTGAACCCCT ATATGGACTT CCCACCTACT GATACTCTGG GATATGGGGG ACCTGAAGGG
    GCAGCAGCTG AGCCTTATGG AGCGAGGGGT CCAGGCTCTC TGCCTCTTGG GCCTGGTCCA
    CCCACCAACT ATGGCCCCAA CCCCTGTCCC CAGCAGGCCT CATATCCTGA CCCCACCCAA
    GAAACATGGG GTGAGTTCCC TTCCCACTCT GGGCTGTACC CAGGCCCCAA GGCTCTAGGT
    GGAACCTACA GCCAGTGTCC TCGACTTGAA CATTATGGAC AAGTGCAAGT CAAGCCAGAA
    CAGGGGTGCC CAGTGGGGTC TGACTCCACA GGACTGGCAC CCTGCCTCAA TGCCCACCCC
    AGTGAGGGGC CCCCACATCC ACAGCCTCTC TTTTCCCATT ACCCCCAGCC CTCTCCTCCC
    CAATATCTCC AGTCAGGCCC CTATACCCAG CCACCCCCTG ATTATCTTCC TTCAGAACCC
    AGGCCTTGCC TGGACTTTGA TTCCCCCACC CATTCCACAG GGCAGCTCAA GGCTCAGCTT
    GTGTGTAATT ATGTTCAATC TCAACAGGAG CTACTGTGGG AGGGTGGGGG CAGGGAAGAT
    GCCCCCGCCC AGGAACCTTC CTACCAGAGT CCCAAGTTTC TGGGGGGTTC CCAGGTTAGC
    CCAAGCCGTG CTAAAGCTCC AGTGAACACA TATGGACCTG GCTTTGGACC CAACTTGCCC
    AATCACAAGT CAGGTTCCTA TCCCACCCCT TCACCATGCC ATGAAAATTT TGTAGTGGGG
    GCAAATAGGG CTTCACATAG GGCAGCAGCA CCACCTCGAC TTCTGCCCCC ATTGCCCACT
    TGCTATGGGC CTCTCAAAGT GGGAGGCACA AACCCCAGCT GTGGTCATCC TGAGGTGGGC
    AGGCTAGGAG GGGGTCCTGC CTTGTACCCT CCTCCCGAAG GACAGGTATG TAACCCCCTG
    GACTCTCTTG ATCTTGACAA CACTCAGCTG GACTTTGTGG CTATTCTGGA TGAGCCCCAG
    GGGCTGAGTC CTCCTCCTTC CCATGATCAG CGGGGCAGCT CTGGACATAC CCCACCTCCC
    TCTGGGCCCC CCAACATGGC TGTGGGCAAC ATGAGTGTCT TACTGAGATC CCTACCTGGG
    GAAACAGAAT TCCTCAACTC TAGTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536492.1
    CDS92..3181
    Translation

    Target ORF information:

    RefSeq Version XM_011538190.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCACAGCCAA GCCCTGGGGC CCAGTCATCC TGCAGCAGTG ACCACTCCCC GGCAGGGAGT
    GCAGCCAATA CAGACAGTGG TGTGGAAATG ACTGGCAATG CAGGGGGCAG CACTGAAGAC
    CTCTCCAGCT TGGACGAGGG ACCTTGCATT GCTGGCACTG GTCTGTCCAC TCTTCGCCGC
    CTTGAGAACC TCAGGCTGGA CCAGCTACAT CAACTCCGGC CAATAGGGAC CCGGGGTCTC
    AAACTGCCCA GCTTGTCCCA CACCGGTACC ACTGTGTCCC GCCGCGTGGG CCCCCCAGTC
    TCTCTTGAAC GCCGCAGCAG CAGCTCCAGC AGCATCAGCT CTGCCTATAC TGTCAGCCGC
    CGCTCCTCCC TGGCCTCTCC TTTCCCCCCT GGCTCCCCAC CAGAGAATGG AGCATCCTCC
    CTGCCTGGCC TTATGCCTGC CCAGCACTAC CTGCTTCGGG CAAGATATGC TTCAGCCAGA
    GGGGGTGGTA CTTCGCCCAC TGCAGCATCC AGCCTGGATC GGATAGGTGG TCTTCCCATG
    CCTCCTTGGA GAAGCCGAGC CGAGTATCCA GGATACAACC CCAATGCAGG GGTCACCCGG
    AGGGCCAGTG ACCCAGCCCA GGCTGCTGAC CGTCCTGCTC CAGCTAGAGT CCAGAGGTTC
    AAGAGCCTGG GCTGTGTCCA TACCCCACCC ACTGTGGCAG GGGGAGGACA GAACTTTGAT
    CCTTACCTCC CAACCTCTGT CTACTCACCA CAGCCCCCCA GCATCACTGA GAATGCTGCC
    ATGGATGCTA GAGGGCTACA GGAAGAGCCA GAAGTTGGGA CCTCCATGGT GGGCAGTGGT
    CTGAACCCCT ATATGGACTT CCCACCTACT GATACTCTGG GATATGGGGG ACCTGAAGGG
    GCAGCAGCTG AGCCTTATGG AGCGAGGGGT CCAGGCTCTC TGCCTCTTGG GCCTGGTCCA
    CCCACCAACT ATGGCCCCAA CCCCTGTCCC CAGCAGGCCT CATATCCTGA CCCCACCCAA
    GAAACATGGG GTGAGTTCCC TTCCCACTCT GGGCTGTACC CAGGCCCCAA GGCTCTAGGT
    GGAACCTACA GCCAGTGTCC TCGACTTGAA CATTATGGAC AAGTGCAAGT CAAGCCAGAA
    CAGGGGTGCC CAGTGGGGTC TGACTCCACA GGACTGGCAC CCTGCCTCAA TGCCCACCCC
    AGTGAGGGGC CCCCACATCC ACAGCCTCTC TTTTCCCATT ACCCCCAGCC CTCTCCTCCC
    CAATATCTCC AGTCAGGCCC CTATACCCAG CCACCCCCTG ATTATCTTCC TTCAGAACCC
    AGGCCTTGCC TGGACTTTGA TTCCCCCACC CATTCCACAG GGCAGCTCAA GGCTCAGCTT
    GTGTGTAATT ATGTTCAATC TCAACAGGAG CTACTGTGGG AGGGTGGGGG CAGGGAAGAT
    GCCCCCGCCC AGGAACCTTC CTACCAGAGT CCCAAGTTTC TGGGGGGTTC CCAGGTTAGC
    CCAAGCCGTG CTAAAGCTCC AGTGAACACA TATGGACCTG GCTTTGGACC CAACTTGCCC
    AATCACAAGT CAGGTTCCTA TCCCACCCCT TCACCATGCC ATGAAAATTT TGTAGTGGGG
    GCAAATAGGG CTTCACATAG GGCAGCAGCA CCACCTCGAC TTCTGCCCCC ATTGCCCACT
    TGCTATGGGC CTCTCAAAGT GGGAGGCACA AACCCCAGCT GTGGTCATCC TGAGGTGGGC
    AGGCTAGGAG GGGGTCCTGC CTTGTACCCT CCTCCCGAAG GACAGGTATG TAACCCCCTG
    GACTCTCTTG ATCTTGACAA CACTCAGCTG GACTTTGTGG CTATTCTGGA TGAGCCCCAG
    GGGCTGAGTC CTCCTCCTTC CCATGATCAG CGGGGCAGCT CTGGACATAC CCCACCTCCC
    TCTGGGCCCC CCAACATGGC TGTGGGCAAC ATGAGTGTCT TACTGAGATC CCTACCTGGG
    GAAACAGAAT TCCTCAACTC TAGTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57981
    Clone ID Related Accession (Same CDS sequence) XM_011538190.1 , XM_011538190.2
    Accession Version XM_011538190.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product zinc finger protein GLI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011538190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCACAGCCAA GCCCTGGGGC CCAGTCATCC TGCAGCAGTG ACCACTCCCC GGCAGGGAGT
    GCAGCCAATA CAGACAGTGG TGTGGAAATG ACTGGCAATG CAGGGGGCAG CACTGAAGAC
    CTCTCCAGCT TGGACGAGGG ACCTTGCATT GCTGGCACTG GTCTGTCCAC TCTTCGCCGC
    CTTGAGAACC TCAGGCTGGA CCAGCTACAT CAACTCCGGC CAATAGGGAC CCGGGGTCTC
    AAACTGCCCA GCTTGTCCCA CACCGGTACC ACTGTGTCCC GCCGCGTGGG CCCCCCAGTC
    TCTCTTGAAC GCCGCAGCAG CAGCTCCAGC AGCATCAGCT CTGCCTATAC TGTCAGCCGC
    CGCTCCTCCC TGGCCTCTCC TTTCCCCCCT GGCTCCCCAC CAGAGAATGG AGCATCCTCC
    CTGCCTGGCC TTATGCCTGC CCAGCACTAC CTGCTTCGGG CAAGATATGC TTCAGCCAGA
    GGGGGTGGTA CTTCGCCCAC TGCAGCATCC AGCCTGGATC GGATAGGTGG TCTTCCCATG
    CCTCCTTGGA GAAGCCGAGC CGAGTATCCA GGATACAACC CCAATGCAGG GGTCACCCGG
    AGGGCCAGTG ACCCAGCCCA GGCTGCTGAC CGTCCTGCTC CAGCTAGAGT CCAGAGGTTC
    AAGAGCCTGG GCTGTGTCCA TACCCCACCC ACTGTGGCAG GGGGAGGACA GAACTTTGAT
    CCTTACCTCC CAACCTCTGT CTACTCACCA CAGCCCCCCA GCATCACTGA GAATGCTGCC
    ATGGATGCTA GAGGGCTACA GGAAGAGCCA GAAGTTGGGA CCTCCATGGT GGGCAGTGGT
    CTGAACCCCT ATATGGACTT CCCACCTACT GATACTCTGG GATATGGGGG ACCTGAAGGG
    GCAGCAGCTG AGCCTTATGG AGCGAGGGGT CCAGGCTCTC TGCCTCTTGG GCCTGGTCCA
    CCCACCAACT ATGGCCCCAA CCCCTGTCCC CAGCAGGCCT CATATCCTGA CCCCACCCAA
    GAAACATGGG GTGAGTTCCC TTCCCACTCT GGGCTGTACC CAGGCCCCAA GGCTCTAGGT
    GGAACCTACA GCCAGTGTCC TCGACTTGAA CATTATGGAC AAGTGCAAGT CAAGCCAGAA
    CAGGGGTGCC CAGTGGGGTC TGACTCCACA GGACTGGCAC CCTGCCTCAA TGCCCACCCC
    AGTGAGGGGC CCCCACATCC ACAGCCTCTC TTTTCCCATT ACCCCCAGCC CTCTCCTCCC
    CAATATCTCC AGTCAGGCCC CTATACCCAG CCACCCCCTG ATTATCTTCC TTCAGAACCC
    AGGCCTTGCC TGGACTTTGA TTCCCCCACC CATTCCACAG GGCAGCTCAA GGCTCAGCTT
    GTGTGTAATT ATGTTCAATC TCAACAGGAG CTACTGTGGG AGGGTGGGGG CAGGGAAGAT
    GCCCCCGCCC AGGAACCTTC CTACCAGAGT CCCAAGTTTC TGGGGGGTTC CCAGGTTAGC
    CCAAGCCGTG CTAAAGCTCC AGTGAACACA TATGGACCTG GCTTTGGACC CAACTTGCCC
    AATCACAAGT CAGGTTCCTA TCCCACCCCT TCACCATGCC ATGAAAATTT TGTAGTGGGG
    GCAAATAGGG CTTCACATAG GGCAGCAGCA CCACCTCGAC TTCTGCCCCC ATTGCCCACT
    TGCTATGGGC CTCTCAAAGT GGGAGGCACA AACCCCAGCT GTGGTCATCC TGAGGTGGGC
    AGGCTAGGAG GGGGTCCTGC CTTGTACCCT CCTCCCGAAG GACAGGTATG TAACCCCCTG
    GACTCTCTTG ATCTTGACAA CACTCAGCTG GACTTTGTGG CTATTCTGGA TGAGCCCCAG
    GGGCTGAGTC CTCCTCCTTC CCATGATCAG CGGGGCAGCT CTGGACATAC CCCACCTCCC
    TCTGGGCCCC CCAACATGGC TGTGGGCAAC ATGAGTGTCT TACTGAGATC CCTACCTGGG
    GAAACAGAAT TCCTCAACTC TAGTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011536492.1
    CDS92..3181
    Translation

    Target ORF information:

    RefSeq Version XM_011538190.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011538190.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTCAACT CGATGACCCC ACCACCAATC AGTAGCTATG GCGAGCCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGTGTG GGGACAGAAG GACTGTCTGG CCCGCCCTTC
    TGCCACCAAG CTAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACCG AGGGCCCACT CTTTTCTTCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTGT CCATCTCACC TCTGTCGGAT GCCAGCCTGG ACCTGCAGAC GGTTATCCGC
    ACCTCACCCA GCTCCCTCGT AGCTTTCATC AACTCGCGAT GCACATCTCC AGGAGGCTCC
    TACGGTCATC TCTCCATTGG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGCCCTCGCC TTCCTTTGGG GTCCAGCCTT GTGGTCCCCA TGACTCTGCC
    CGGGGTGGGA TGATCCCACA TCCTCAGTCC CGGGGACCCT TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TGGCAAGTGC CGGGAGGAAC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACAGGCAT ACAGGATCCC CTGTTGGGGA TGCTGGATGG GCGGGAGGAC
    CTCGAGAGAG AGGAGAAGCG TGAGCCTGAA TCTGTGTATG AAACTGACTG CCGTTGGGAT
    GGCTGCAGCC AGGAATTTGA CTCCCAAGAG CAGCTGGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CACTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTT CACATGCGCA GACACACTGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAAGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA CCTGAAGACG
    CACCTGCGGT CACACACGGG TGAGAAGCCA TACATGTGTG AGCACGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCACAGCCAA GCCCTGGGGC CCAGTCATCC TGCAGCAGTG ACCACTCCCC GGCAGGGAGT
    GCAGCCAATA CAGACAGTGG TGTGGAAATG ACTGGCAATG CAGGGGGCAG CACTGAAGAC
    CTCTCCAGCT TGGACGAGGG ACCTTGCATT GCTGGCACTG GTCTGTCCAC TCTTCGCCGC
    CTTGAGAACC TCAGGCTGGA CCAGCTACAT CAACTCCGGC CAATAGGGAC CCGGGGTCTC
    AAACTGCCCA GCTTGTCCCA CACCGGTACC ACTGTGTCCC GCCGCGTGGG CCCCCCAGTC
    TCTCTTGAAC GCCGCAGCAG CAGCTCCAGC AGCATCAGCT CTGCCTATAC TGTCAGCCGC
    CGCTCCTCCC TGGCCTCTCC TTTCCCCCCT GGCTCCCCAC CAGAGAATGG AGCATCCTCC
    CTGCCTGGCC TTATGCCTGC CCAGCACTAC CTGCTTCGGG CAAGATATGC TTCAGCCAGA
    GGGGGTGGTA CTTCGCCCAC TGCAGCATCC AGCCTGGATC GGATAGGTGG TCTTCCCATG
    CCTCCTTGGA GAAGCCGAGC CGAGTATCCA GGATACAACC CCAATGCAGG GGTCACCCGG
    AGGGCCAGTG ACCCAGCCCA GGCTGCTGAC CGTCCTGCTC CAGCTAGAGT CCAGAGGTTC
    AAGAGCCTGG GCTGTGTCCA TACCCCACCC ACTGTGGCAG GGGGAGGACA GAACTTTGAT
    CCTTACCTCC CAACCTCTGT CTACTCACCA CAGCCCCCCA GCATCACTGA GAATGCTGCC
    ATGGATGCTA GAGGGCTACA GGAAGAGCCA GAAGTTGGGA CCTCCATGGT GGGCAGTGGT
    CTGAACCCCT ATATGGACTT CCCACCTACT GATACTCTGG GATATGGGGG ACCTGAAGGG
    GCAGCAGCTG AGCCTTATGG AGCGAGGGGT CCAGGCTCTC TGCCTCTTGG GCCTGGTCCA
    CCCACCAACT ATGGCCCCAA CCCCTGTCCC CAGCAGGCCT CATATCCTGA CCCCACCCAA
    GAAACATGGG GTGAGTTCCC TTCCCACTCT GGGCTGTACC CAGGCCCCAA GGCTCTAGGT
    GGAACCTACA GCCAGTGTCC TCGACTTGAA CATTATGGAC AAGTGCAAGT CAAGCCAGAA
    CAGGGGTGCC CAGTGGGGTC TGACTCCACA GGACTGGCAC CCTGCCTCAA TGCCCACCCC
    AGTGAGGGGC CCCCACATCC ACAGCCTCTC TTTTCCCATT ACCCCCAGCC CTCTCCTCCC
    CAATATCTCC AGTCAGGCCC CTATACCCAG CCACCCCCTG ATTATCTTCC TTCAGAACCC
    AGGCCTTGCC TGGACTTTGA TTCCCCCACC CATTCCACAG GGCAGCTCAA GGCTCAGCTT
    GTGTGTAATT ATGTTCAATC TCAACAGGAG CTACTGTGGG AGGGTGGGGG CAGGGAAGAT
    GCCCCCGCCC AGGAACCTTC CTACCAGAGT CCCAAGTTTC TGGGGGGTTC CCAGGTTAGC
    CCAAGCCGTG CTAAAGCTCC AGTGAACACA TATGGACCTG GCTTTGGACC CAACTTGCCC
    AATCACAAGT CAGGTTCCTA TCCCACCCCT TCACCATGCC ATGAAAATTT TGTAGTGGGG
    GCAAATAGGG CTTCACATAG GGCAGCAGCA CCACCTCGAC TTCTGCCCCC ATTGCCCACT
    TGCTATGGGC CTCTCAAAGT GGGAGGCACA AACCCCAGCT GTGGTCATCC TGAGGTGGGC
    AGGCTAGGAG GGGGTCCTGC CTTGTACCCT CCTCCCGAAG GACAGGTATG TAACCCCCTG
    GACTCTCTTG ATCTTGACAA CACTCAGCTG GACTTTGTGG CTATTCTGGA TGAGCCCCAG
    GGGCTGAGTC CTCCTCCTTC CCATGATCAG CGGGGCAGCT CTGGACATAC CCCACCTCCC
    TCTGGGCCCC CCAACATGGC TGTGGGCAAC ATGAGTGTCT TACTGAGATC CCTACCTGGG
    GAAACAGAAT TCCTCAACTC TAGTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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