GLI1 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following GLI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa90750 XM_015471088.1
Latest version!
Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa19428 NM_001099000.1
Latest version!
Bos taurus GLI family zinc finger 1 (GLI1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa19428 XM_010805169.3
Latest version!
Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa19428 XM_015471089.1
Latest version!
Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa19428 XM_005206586.4
Latest version!
Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa19428 XM_010805168.3
Latest version!
Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa19428 XM_010805168.2 Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa19428 XM_005206586.3 Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa19428 XM_010805169.2 Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa90750
    Clone ID Related Accession (Same CDS sequence) XM_015471088.1
    Accession Version XM_015471088.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3489bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAGCC CTGTTGTCTC CGGCCCCTCC CCAGTCAGGG GGCCCCCGGC ATGGGGACAG 
    AAGGTCAGTA CATACACCAG TCCCTGGACC TTGACTACTG GCAGATCTCT CACCCAGCCC
    CAGGGGTCCC TCTTCTATCC CATTCCAGTT TCCATATACA AAAAGATTTG CGGTCCCATG
    CCATATGGGA TTGGGCCTTA CTTGGGCTTT CAAGATGGAC TGCATGTGTC CTTCATTCCC
    ATGCCCCATT CCAGCTGTAT CTCTTTCCTA GGACTGCCAG GTCTGCCCTT CTGTCATCAG
    GCCACCCTCA TGCCCGGCCC CCACAGTTAT GGGCCAGCCA GAGAAGCCAA CAGCTGCACG
    GAGGTGCCAC TGTTTCCTCC TCCCCGGAGT GCAGTCAAAT TGACCAAGAA GCGGGCACTC
    TCCATCTCAC CTCTGTCGGA CACCAGCCTG GACCTGCAGA CAGTTATCCG CACCTCACCC
    AGCTCCCTCG TGGCCTTCAT CAACTCACGC TGTGCATCTC CGGGGGGTTC CTATGGTCAC
    CTCTCCATCG GCACCATGAG CCCATCTCTG GGATTCTCAC CCCAGATGAA CCACCAGAAA
    GGGACCTCAC CTTCTTTTGG GGTCCAGCCC TGTGGTCCCC ATGACCCCAC GCAGAGTGGG
    ATGATGCCGC ATCCTCAGTC CCGGGGACCC CTCCCAACGT GCCAGCTGAA GTCTGAGCTG
    GGTGTGCTGG TTAACAAGTG CCCGGAGGAG CCCTTGGAAG GTGATATGTC CAGTCCCAAC
    TCCTCGAGCA CACAGGATCC ACTGCTGGGG ATGCTGGATG GGCGGGAGGA CCTAGAGAGA
    GAAGAGAAAC CTGAGCCTGA CTCTGTGTAT GAGACTGACT GTCGCTGGGA TGGCTGCAGT
    CAGGAATTCG ACTCCCAGGA GCAACTGGTG CATCACATCA ACAGTGAGCA CATCCATGGA
    GAGCGGAAGG AGTTCGTGTG CCATTGGGGG GGCTGCTCCA GGGAGCTGAG GCCCTTCAAA
    GCCCAGTACA TGCTGGTGGT GCACATGCGC AGACACACGG GCGAGAAGCC ACATAAGTGC
    ACGTTTGAGG GGTGCCGGAA GTCATACTCA CGCCTGGAAA ATCTGAAGAC GCACCTGCGG
    TCACACACTG GTGAGAAGCC ATACATGTGT GAGCATGAGG GCTGCAGTAA AGCCTTCAGC
    AATGCCAGTG ACCGAGCCAA GCACCAGAAT CGGACCCATT CCAATGAGAA GCCCTACGTG
    TGTAAACTCC CTGGCTGCAC CAAACGCTAC ACAGATCCCA GCTCGCTCAG GAAACATGTC
    AAGACAGTAC ACGGTCCTGA TGCCCACGTG ACCAAGCGGC ATCGAGGAGA CGGCCCCCTG
    CCCCGGGCAC CGGCTCTTTC CTCAGTGGAA CCCAAGAGGG AGCGGGACGG AGGCCCCATT
    AGGGAGGAGA GCAGACTGAC TGTGCCGGAG GGTGCCATGA AGCCACAGCC GAGTCCTGGG
    GCCCAGTCGT CCTGCAGCAG TGACCACTCC CCAGCAGGCA GCGCGGCCAA TACAGACAGC
    GGTGTGGAAA TGACTGGAAA TGCAGGGGGC AGCACGGAGG ACCTGTCCAG CTTGGATGAG
    GGGCCTTGCC TTGCGGGAAC CGGGCTGTCT ACTCTTCGCC GCCTTGAGAA CCTCAGGCTG
    GACCAGCTAC ATCAGTTCCG GCCGATGGGG CCCCGGGGCC TCAAACTGCC CAGCTTGACC
    CACACTGGCA CTCCTGGCTC TCGCCGTCTG GGCGCCCCAG TATCTCTTGA CCGCCGCAGC
    AGCAGCTCCA GCAGTGTCAG TTCAGCCTAT ACTGTCAGCC GCCGCTCCTC CCTTGCCTCC
    CCTTTCCCCC CTGGTTCCCC ACCAGAGAAC GGGGCATCCT CTCTGCCTGG CCTCACGCCT
    GCCCAGCACT ACCTGCTCCG GGCCAGATAT GCTTCAGCCA GGGGAGGTGG TACCCCACCC
    ACTGCAGCAC CCAGCCTGGA TCGGACGGGG GCTCTTCCTG CACCTCCCTG GAGAAGCCGA
    GCTGAGTATC CAGGATACAA CCCCAATGTA GGGGTCACCC GGAGAGCCAG TGACCCAGCC
    CGATCTGTTG ACCGCCCTGC CCCAGCCAGA GTCCAGAGGT TCAAGAGTTT GGGCTGTGTC
    CACAACACCC CCACAGTGGT AGGAGGAGGA CAGAACTTCG ATCCCCAACT CCCAACCTCT
    GTCTACTCAC CACAGCCCCC CAGCATCACT GAGAATGTCT CCATGGATAC CAGAGGGCTA
    CGCGAGGAGC CAGAGGTTGG GACCTCCATG ATGGGCAGTG GTCTGAACCC CTATATGGAC
    TTCCCACCTG CTGATACTCT GGGATATGGA GGGCCTGAGG CGGCAGCAGC TGAGCCTTAT
    GGAGCTAGAG GGCCCGGCTC CCTGCCTCTT GGGCCTGGTC CACCCACCAA CTATGGCCCC
    AACCCCTGTC CCCAGCAGGT TCCCTATTCT GAACCAACCC CAGAAACATG GAGTGAGTTC
    CCTTCCCACT CTGGGCTCTA CTCAGGCCCC AAGGCTCCAG CTGGAGCCTA CAGCCAGTGT
    CCTCGTCTTG AACATTATGG ACAAGTGCAA GTGAAGCCAG AACAGGGGTG CCCCGTGGGT
    TCTGACGCCA CAGGACTGGC ACCCTGCCTC AATGCCCACC CCAATGAGGG GCCTCCCCGC
    TCACAGCCTC TGTTCTCCCA CTACCCACAG CCTCCCCCTC CCCAGTATCC CCAGTCAGGT
    TCCTACACCC AGCCACCTCC TGATTATCTG CCTTCAGAAG CCAGGCCCAG CCTGGATTTC
    GAGTCCCCCA CTCATTCTAC AGGACACCTC AAGGCGCAGC TTGTGTGTAA TTATGTTCAG
    TCTCAACAGG AGCTGCTGTG GGACAGTGGA GGCAGGGGAG ACCCTCCAGT CCAAGAACCT
    CTCTATCAGA GTCCCAAGTT TCTGGGGGGT TCCCAGGTCA GCCCAAGCCC TGCCAAGGCC
    CCAGTGGCCA CATATGGACC TGGCTTTGCA CCTAATATGC CCAATCACAA GCCAGGCTCC
    TATCCTACCC CTTCATGCCA TGAAAATTTT GCAGTAGGGG CCAACAAAGC TTCCCATAGG
    GCAGCAGCAC CACCCCGACT TCTACCCCCA CTACCGGCTT GCTATGGGCC CCTCAAGGCA
    GGGGGCACCA ACCCCAGCTG TGGCCATCCT GAGGTGGGCA GGCTGGGAGG GGGCCCCGCC
    TTGTACCCTC CTCCTGAAGG GCAGGTGTGT AACCCTCTGG ACTCTCTTGA TCTCGACAAC
    ACGCAGCTGG ACTTTGTGGC TATTCTGGAT GAGGCCCAGG GGCTGAGTCC TCCCCCTTCT
    CACAATCAGA GGGACAGCTC TGAACACACC CCACCTCCCT CTGGGACCCC TAACATGGCC
    GTGGGCAACA TGAGTGTGTT ACTAGGATCT CTGCCTGGGG AGACACAGTT CCTCAATTCT
    AGTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326574.1
    CDS31..3519
    Translation

    Target ORF information:

    RefSeq Version XM_015471088.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471088.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAGCC CTGTTGTCTC CGGCCCCTCC CCAGTCAGGG GGCCCCCGGC ATGGGGACAG 
    AAGGTCAGTA CATACACCAG TCCCTGGACC TTGACTACTG GCAGATCTCT CACCCAGCCC
    CAGGGGTCCC TCTTCTATCC CATTCCAGTT TCCATATACA AAAAGATTTG CGGTCCCATG
    CCATATGGGA TTGGGCCTTA CTTGGGCTTT CAAGATGGAC TGCATGTGTC CTTCATTCCC
    ATGCCCCATT CCAGCTGTAT CTCTTTCCTA GGACTGCCAG GTCTGCCCTT CTGTCATCAG
    GCCACCCTCA TGCCCGGCCC CCACAGTTAT GGGCCAGCCA GAGAAGCCAA CAGCTGCACG
    GAGGTGCCAC TGTTTCCTCC TCCCCGGAGT GCAGTCAAAT TGACCAAGAA GCGGGCACTC
    TCCATCTCAC CTCTGTCGGA CACCAGCCTG GACCTGCAGA CAGTTATCCG CACCTCACCC
    AGCTCCCTCG TGGCCTTCAT CAACTCACGC TGTGCATCTC CGGGGGGTTC CTATGGTCAC
    CTCTCCATCG GCACCATGAG CCCATCTCTG GGATTCTCAC CCCAGATGAA CCACCAGAAA
    GGGACCTCAC CTTCTTTTGG GGTCCAGCCC TGTGGTCCCC ATGACCCCAC GCAGAGTGGG
    ATGATGCCGC ATCCTCAGTC CCGGGGACCC CTCCCAACGT GCCAGCTGAA GTCTGAGCTG
    GGTGTGCTGG TTAACAAGTG CCCGGAGGAG CCCTTGGAAG GTGATATGTC CAGTCCCAAC
    TCCTCGAGCA CACAGGATCC ACTGCTGGGG ATGCTGGATG GGCGGGAGGA CCTAGAGAGA
    GAAGAGAAAC CTGAGCCTGA CTCTGTGTAT GAGACTGACT GTCGCTGGGA TGGCTGCAGT
    CAGGAATTCG ACTCCCAGGA GCAACTGGTG CATCACATCA ACAGTGAGCA CATCCATGGA
    GAGCGGAAGG AGTTCGTGTG CCATTGGGGG GGCTGCTCCA GGGAGCTGAG GCCCTTCAAA
    GCCCAGTACA TGCTGGTGGT GCACATGCGC AGACACACGG GCGAGAAGCC ACATAAGTGC
    ACGTTTGAGG GGTGCCGGAA GTCATACTCA CGCCTGGAAA ATCTGAAGAC GCACCTGCGG
    TCACACACTG GTGAGAAGCC ATACATGTGT GAGCATGAGG GCTGCAGTAA AGCCTTCAGC
    AATGCCAGTG ACCGAGCCAA GCACCAGAAT CGGACCCATT CCAATGAGAA GCCCTACGTG
    TGTAAACTCC CTGGCTGCAC CAAACGCTAC ACAGATCCCA GCTCGCTCAG GAAACATGTC
    AAGACAGTAC ACGGTCCTGA TGCCCACGTG ACCAAGCGGC ATCGAGGAGA CGGCCCCCTG
    CCCCGGGCAC CGGCTCTTTC CTCAGTGGAA CCCAAGAGGG AGCGGGACGG AGGCCCCATT
    AGGGAGGAGA GCAGACTGAC TGTGCCGGAG GGTGCCATGA AGCCACAGCC GAGTCCTGGG
    GCCCAGTCGT CCTGCAGCAG TGACCACTCC CCAGCAGGCA GCGCGGCCAA TACAGACAGC
    GGTGTGGAAA TGACTGGAAA TGCAGGGGGC AGCACGGAGG ACCTGTCCAG CTTGGATGAG
    GGGCCTTGCC TTGCGGGAAC CGGGCTGTCT ACTCTTCGCC GCCTTGAGAA CCTCAGGCTG
    GACCAGCTAC ATCAGTTCCG GCCGATGGGG CCCCGGGGCC TCAAACTGCC CAGCTTGACC
    CACACTGGCA CTCCTGGCTC TCGCCGTCTG GGCGCCCCAG TATCTCTTGA CCGCCGCAGC
    AGCAGCTCCA GCAGTGTCAG TTCAGCCTAT ACTGTCAGCC GCCGCTCCTC CCTTGCCTCC
    CCTTTCCCCC CTGGTTCCCC ACCAGAGAAC GGGGCATCCT CTCTGCCTGG CCTCACGCCT
    GCCCAGCACT ACCTGCTCCG GGCCAGATAT GCTTCAGCCA GGGGAGGTGG TACCCCACCC
    ACTGCAGCAC CCAGCCTGGA TCGGACGGGG GCTCTTCCTG CACCTCCCTG GAGAAGCCGA
    GCTGAGTATC CAGGATACAA CCCCAATGTA GGGGTCACCC GGAGAGCCAG TGACCCAGCC
    CGATCTGTTG ACCGCCCTGC CCCAGCCAGA GTCCAGAGGT TCAAGAGTTT GGGCTGTGTC
    CACAACACCC CCACAGTGGT AGGAGGAGGA CAGAACTTCG ATCCCCAACT CCCAACCTCT
    GTCTACTCAC CACAGCCCCC CAGCATCACT GAGAATGTCT CCATGGATAC CAGAGGGCTA
    CGCGAGGAGC CAGAGGTTGG GACCTCCATG ATGGGCAGTG GTCTGAACCC CTATATGGAC
    TTCCCACCTG CTGATACTCT GGGATATGGA GGGCCTGAGG CGGCAGCAGC TGAGCCTTAT
    GGAGCTAGAG GGCCCGGCTC CCTGCCTCTT GGGCCTGGTC CACCCACCAA CTATGGCCCC
    AACCCCTGTC CCCAGCAGGT TCCCTATTCT GAACCAACCC CAGAAACATG GAGTGAGTTC
    CCTTCCCACT CTGGGCTCTA CTCAGGCCCC AAGGCTCCAG CTGGAGCCTA CAGCCAGTGT
    CCTCGTCTTG AACATTATGG ACAAGTGCAA GTGAAGCCAG AACAGGGGTG CCCCGTGGGT
    TCTGACGCCA CAGGACTGGC ACCCTGCCTC AATGCCCACC CCAATGAGGG GCCTCCCCGC
    TCACAGCCTC TGTTCTCCCA CTACCCACAG CCTCCCCCTC CCCAGTATCC CCAGTCAGGT
    TCCTACACCC AGCCACCTCC TGATTATCTG CCTTCAGAAG CCAGGCCCAG CCTGGATTTC
    GAGTCCCCCA CTCATTCTAC AGGACACCTC AAGGCGCAGC TTGTGTGTAA TTATGTTCAG
    TCTCAACAGG AGCTGCTGTG GGACAGTGGA GGCAGGGGAG ACCCTCCAGT CCAAGAACCT
    CTCTATCAGA GTCCCAAGTT TCTGGGGGGT TCCCAGGTCA GCCCAAGCCC TGCCAAGGCC
    CCAGTGGCCA CATATGGACC TGGCTTTGCA CCTAATATGC CCAATCACAA GCCAGGCTCC
    TATCCTACCC CTTCATGCCA TGAAAATTTT GCAGTAGGGG CCAACAAAGC TTCCCATAGG
    GCAGCAGCAC CACCCCGACT TCTACCCCCA CTACCGGCTT GCTATGGGCC CCTCAAGGCA
    GGGGGCACCA ACCCCAGCTG TGGCCATCCT GAGGTGGGCA GGCTGGGAGG GGGCCCCGCC
    TTGTACCCTC CTCCTGAAGG GCAGGTGTGT AACCCTCTGG ACTCTCTTGA TCTCGACAAC
    ACGCAGCTGG ACTTTGTGGC TATTCTGGAT GAGGCCCAGG GGCTGAGTCC TCCCCCTTCT
    CACAATCAGA GGGACAGCTC TGAACACACC CCACCTCCCT CTGGGACCCC TAACATGGCC
    GTGGGCAACA TGAGTGTGTT ACTAGGATCT CTGCCTGGGG AGACACAGTT CCTCAATTCT
    AGTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19428
    Clone ID Related Accession (Same CDS sequence) XM_010805169.2 , XM_005206586.3 , XM_010805168.3 , NM_001099000.1 , XM_010805168.2 , XM_005206586.4 , XM_010805169.3 , XM_015471089.1
    Accession Version NM_001099000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC146090.1. On Jun 29, 2007 this sequence version replaced XM_595760.3. ##Evidence-Data-START## Transcript exon combination :: BC146090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145429, SAMN03145432 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001092470.1
    CDS78..3395
    Translation

    Target ORF information:

    RefSeq Version NM_001099000.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 1 (GLI1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001099000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19428
    Clone ID Related Accession (Same CDS sequence) XM_010805169.2 , XM_005206586.3 , XM_010805168.3 , NM_001099000.1 , XM_010805168.2 , XM_005206586.4 , XM_010805169.3 , XM_015471089.1
    Accession Version XM_010805169.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803471.1
    CDS135..3452
    Translation

    Target ORF information:

    RefSeq Version XM_010805169.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805169.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19428
    Clone ID Related Accession (Same CDS sequence) XM_010805169.2 , XM_005206586.3 , XM_010805168.3 , NM_001099000.1 , XM_010805168.2 , XM_005206586.4 , XM_010805169.3 , XM_015471089.1
    Accession Version XM_005206586.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206586.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206643.1
    CDS284..3601
    Translation

    Target ORF information:

    RefSeq Version XM_005206586.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206586.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19428
    Clone ID Related Accession (Same CDS sequence) XM_010805169.2 , XM_005206586.3 , XM_010805168.3 , NM_001099000.1 , XM_010805168.2 , XM_005206586.4 , XM_010805169.3 , XM_015471089.1
    Accession Version XM_010805168.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805168.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803470.1
    CDS354..3671
    Translation

    Target ORF information:

    RefSeq Version XM_010805168.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805168.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19428
    Clone ID Related Accession (Same CDS sequence) XM_010805169.2 , XM_005206586.3 , XM_010805168.3 , NM_001099000.1 , XM_010805168.2 , XM_005206586.4 , XM_010805169.3 , XM_015471089.1
    Accession Version XM_010805168.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803470.1
    CDS305..3622
    Translation

    Target ORF information:

    RefSeq Version XM_010805168.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805168.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19428
    Clone ID Related Accession (Same CDS sequence) XM_010805169.2 , XM_005206586.3 , XM_010805168.3 , NM_001099000.1 , XM_010805168.2 , XM_005206586.4 , XM_010805169.3 , XM_015471089.1
    Accession Version XM_005206586.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206586.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206643.1
    CDS333..3650
    Translation

    Target ORF information:

    RefSeq Version XM_005206586.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206586.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19428
    Clone ID Related Accession (Same CDS sequence) XM_010805169.2 , XM_005206586.3 , XM_010805168.3 , NM_001099000.1 , XM_010805168.2 , XM_005206586.4 , XM_010805169.3 , XM_015471089.1
    Accession Version XM_010805169.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803471.1
    CDS186..3503
    Translation

    Target ORF information:

    RefSeq Version XM_010805169.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805169.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19428
    Clone ID Related Accession (Same CDS sequence) XM_010805169.2 , XM_005206586.3 , XM_010805168.3 , NM_001099000.1 , XM_010805168.2 , XM_005206586.4 , XM_010805169.3 , XM_015471089.1
    Accession Version XM_015471089.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product zinc finger protein GLI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326575.1
    CDS677..3994
    Translation

    Target ORF information:

    RefSeq Version XM_015471089.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471089.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAGCCCTG TTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCGGCATG GGGACAGAAG GACTGCCAGG TCTGCCCTTC
    TGTCATCAGG CCACCCTCAT GCCCGGCCCC CACAGTTATG GGCCAGCCAG AGAAGCCAAC
    AGCTGCACGG AGGTGCCACT GTTTCCTCCT CCCCGGAGTG CAGTCAAATT GACCAAGAAG
    CGGGCACTCT CCATCTCACC TCTGTCGGAC ACCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCACCCA GCTCCCTCGT GGCCTTCATC AACTCACGCT GTGCATCTCC GGGGGGTTCC
    TATGGTCACC TCTCCATCGG CACCATGAGC CCATCTCTGG GATTCTCACC CCAGATGAAC
    CACCAGAAAG GGACCTCACC TTCTTTTGGG GTCCAGCCCT GTGGTCCCCA TGACCCCACG
    CAGAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACGTG CCAGCTGAAG
    TCTGAGCTGG GTGTGCTGGT TAACAAGTGC CCGGAGGAGC CCTTGGAAGG TGATATGTCC
    AGTCCCAACT CCTCGAGCAC ACAGGATCCA CTGCTGGGGA TGCTGGATGG GCGGGAGGAC
    CTAGAGAGAG AAGAGAAACC TGAGCCTGAC TCTGTGTATG AGACTGACTG TCGCTGGGAT
    GGCTGCAGTC AGGAATTCGA CTCCCAGGAG CAACTGGTGC ATCACATCAA CAGTGAGCAC
    ATCCATGGAG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCAG GGAGCTGAGG
    CCCTTCAAAG CCCAGTACAT GCTGGTGGTG CACATGCGCA GACACACGGG CGAGAAGCCA
    CATAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTGGAAAA TCTGAAGACG
    CACCTGCGGT CACACACTGG TGAGAAGCCA TACATGTGTG AGCATGAGGG CTGCAGTAAA
    GCCTTCAGCA ATGCCAGTGA CCGAGCCAAG CACCAGAATC GGACCCATTC CAATGAGAAG
    CCCTACGTGT GTAAACTCCC TGGCTGCACC AAACGCTACA CAGATCCCAG CTCGCTCAGG
    AAACATGTCA AGACAGTACA CGGTCCTGAT GCCCACGTGA CCAAGCGGCA TCGAGGAGAC
    GGCCCCCTGC CCCGGGCACC GGCTCTTTCC TCAGTGGAAC CCAAGAGGGA GCGGGACGGA
    GGCCCCATTA GGGAGGAGAG CAGACTGACT GTGCCGGAGG GTGCCATGAA GCCACAGCCG
    AGTCCTGGGG CCCAGTCGTC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCGGCCAAT
    ACAGACAGCG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACGGAGGA CCTGTCCAGC
    TTGGATGAGG GGCCTTGCCT TGCGGGAACC GGGCTGTCTA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAGTTCCGG CCGATGGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ACACTGGCAC TCCTGGCTCT CGCCGTCTGG GCGCCCCAGT ATCTCTTGAC
    CGCCGCAGCA GCAGCTCCAG CAGTGTCAGT TCAGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTTGCCTCCC CTTTCCCCCC TGGTTCCCCA CCAGAGAACG GGGCATCCTC TCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCCAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGACGGGGG CTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGATACAAC CCCAATGTAG GGGTCACCCG GAGAGCCAGT
    GACCCAGCCC GATCTGTTGA CCGCCCTGCC CCAGCCAGAG TCCAGAGGTT CAAGAGTTTG
    GGCTGTGTCC ACAACACCCC CACAGTGGTA GGAGGAGGAC AGAACTTCGA TCCCCAACTC
    CCAACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATGTCTC CATGGATACC
    AGAGGGCTAC GCGAGGAGCC AGAGGTTGGG ACCTCCATGA TGGGCAGTGG TCTGAACCCC
    TATATGGACT TCCCACCTGC TGATACTCTG GGATATGGAG GGCCTGAGGC GGCAGCAGCT
    GAGCCTTATG GAGCTAGAGG GCCCGGCTCC CTGCCTCTTG GGCCTGGTCC ACCCACCAAC
    TATGGCCCCA ACCCCTGTCC CCAGCAGGTT CCCTATTCTG AACCAACCCC AGAAACATGG
    AGTGAGTTCC CTTCCCACTC TGGGCTCTAC TCAGGCCCCA AGGCTCCAGC TGGAGCCTAC
    AGCCAGTGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TGAAGCCAGA ACAGGGGTGC
    CCCGTGGGTT CTGACGCCAC AGGACTGGCA CCCTGCCTCA ATGCCCACCC CAATGAGGGG
    CCTCCCCGCT CACAGCCTCT GTTCTCCCAC TACCCACAGC CTCCCCCTCC CCAGTATCCC
    CAGTCAGGTT CCTACACCCA GCCACCTCCT GATTATCTGC CTTCAGAAGC CAGGCCCAGC
    CTGGATTTCG AGTCCCCCAC TCATTCTACA GGACACCTCA AGGCGCAGCT TGTGTGTAAT
    TATGTTCAGT CTCAACAGGA GCTGCTGTGG GACAGTGGAG GCAGGGGAGA CCCTCCAGTC
    CAAGAACCTC TCTATCAGAG TCCCAAGTTT CTGGGGGGTT CCCAGGTCAG CCCAAGCCCT
    GCCAAGGCCC CAGTGGCCAC ATATGGACCT GGCTTTGCAC CTAATATGCC CAATCACAAG
    CCAGGCTCCT ATCCTACCCC TTCATGCCAT GAAAATTTTG CAGTAGGGGC CAACAAAGCT
    TCCCATAGGG CAGCAGCACC ACCCCGACTT CTACCCCCAC TACCGGCTTG CTATGGGCCC
    CTCAAGGCAG GGGGCACCAA CCCCAGCTGT GGCCATCCTG AGGTGGGCAG GCTGGGAGGG
    GGCCCCGCCT TGTACCCTCC TCCTGAAGGG CAGGTGTGTA ACCCTCTGGA CTCTCTTGAT
    CTCGACAACA CGCAGCTGGA CTTTGTGGCT ATTCTGGATG AGGCCCAGGG GCTGAGTCCT
    CCCCCTTCTC ACAATCAGAG GGACAGCTCT GAACACACCC CACCTCCCTC TGGGACCCCT
    AACATGGCCG TGGGCAACAT GAGTGTGTTA CTAGGATCTC TGCCTGGGGA GACACAGTTC
    CTCAATTCTA GTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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