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HGF cDNA ORF clone, Homo sapiens(Human)

Gene Symbol HGF
Entrez Gene ID 3082
Full Name hepatocyte growth factor
Synonyms DFNB39,F-TCF,HGFB,HPTA,SF
General protein information
Preferred Names

hepatocyte growth factor

Names

hepatocyte growth factor
fibroblast-derived tumor cytotoxic factor
hepatocyte growth factor (hepapoietin A; scatter factor)
hepatopoietin-A
lung fibroblast-derived mitogen

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7q21.11

Summary This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015].
Disorder MIM:

142409

Disorder Html: Deafness, autosomal recessive 39, 608265 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001010931.2 NP_001010931.1 hepatocyte growth factor isoform 2 precursor
NM_001010934.2 NP_001010934.1 hepatocyte growth factor isoform 5 precursor
NM_001010932.2 NP_001010932.1 hepatocyte growth factor isoform 3 preproprotein
NM_000601.4 NP_000592.3 hepatocyte growth factor isoform 1 preproprotein
XM_006715956.2 XP_006716019.1 hepatocyte growth factor isoform X1
XM_017012097.1 XP_016867586.1 hepatocyte growth factor isoform X3
NM_001010933.1 NP_001010933.1 hepatocyte growth factor isoform 4 precursor
NM_000601.5 NP_000592.3 hepatocyte growth factor isoform 1 preproprotein
NM_001010933.2 NP_001010933.1 hepatocyte growth factor isoform 4 precursor
NM_001010931.1 NP_001010931.1 hepatocyte growth factor isoform 2 precursor
XM_011516115.2 XP_011514417.1 hepatocyte growth factor isoform X2
XM_017012098.1 XP_016867587.1 hepatocyte growth factor isoform X4



Canis lupus familiaris (dog) HGF NP_001002964.1
Rattus norvegicus (Norway rat) Hgf NP_058713.1
Macaca mulatta (rhesus monkey) HGF XP_001108226.2
Bos taurus (cattle) HGF NP_001026921.1
Homo sapiens (human) HGF NP_000592.3
Pan troglodytes (chimpanzee) HGF XP_519174.2
Mus musculus (house mouse) Hgf NP_034557.3
Xenopus tropicalis (tropical clawed frog) hgf XP_002933142.2
Gallus gallus (chicken) HGF NP_001025541.2
Danio rerio (zebrafish) hgfa NP_001013292.1


Related articles in PubMed

Increased HGF Expression Induces Resistance to c-MET Tyrosine Kinase Inhibitors in Gastric Cancer.
Ahn SY, Kim J, Kim MA, Choi J, Kim WH
Anticancer research37(3)1127-1138(2017 03)

Dual inhibition using cabozantinib overcomes HGF/MET signaling mediated resistance to pan-VEGFR inhibition in orthotopic and metastatic neuroblastoma tumors.
Daudigeos-Dubus E, Le Dret L, Bawa O, Opolon P, Vievard A, Villa I, Bosq J, Vassal G, Geoerger B
International journal of oncology50(1)203-211(2017 Jan)

Plasma hepatocyte growth factor is a novel marker of AL cardiac amyloidosis.
Swiger KJ, Friedman EA, Brittain EL, Tomasek KA, Huang S, Su YR, Sawyer DB, Lenihan DJ
Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis23(4)242-248(2016 Dec)

Varying effects of EGF, HGF and TGFβ on formation of invadopodia and invasiveness of melanoma cell lines of different origin.
Makowiecka A, Simiczyjew A, Nowak D, Mazur AJ
European journal of histochemistry : EJH60(4)2728(2016 Dec)

Hepatocyte growth factor twenty years on: Much more than a growth factor.
Nakamura T, Sakai K, Nakamura T, Matsumoto K
Journal of gastroenterology and hepatology26 Suppl 1188-202(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that paxillin appears to influence major cell functions in a diverse range of prostate and breast cancer models. The responsiveness of cells to environmental factors such as HGF or BME may be influenced by paxillin status, although this seems to be dependent on cell type
Title: Potential Implication of Paxillin in Cancer Establishment Within the Bone Environment.

In summary, a decrease in VEGF-A and an increase in sVEGFR1 during chemotherapy and bevacizumab exposure can contribute to both chemotherapy (due to c-MET/b-catenin activation) and bevacizumab (due to low VEGF requirements) resistance. Because HGF levels decrease also during acquired resistance, alternative strategies to HGF-ligand inhibition should be investigated
Title: Dynamic soluble changes in sVEGFR1, HGF, and VEGF promote chemotherapy and bevacizumab resistance: A prospective translational study in the BECOX (GEMCAD 09-01) trial.

Enzyme-linked immunosorbent assay was used to detect the levels of chemokine (C-X-C motif) ligand 12, chemokine (C-X-C motif) ligand 7, hepatocyte growth factor, and fibroblast growth factor 1 in the supernatants of the laryngeal squamous cell carcinoma and control cells.
Title: The primary growth of laryngeal squamous cell carcinoma cells in vitro is effectively supported by paired cancer-associated fibroblasts alone.

overexpression of HGF resulted in resistance to c-MET tyrosine kinase inhibitors through an autocrine manner in gastric cancer cells
Title: Increased HGF Expression Induces Resistance to c-MET Tyrosine Kinase Inhibitors in Gastric Cancer.

activation of HGF/c-MET signaling in an autocrine fashion leads to an aggressive phenotype in SS and targeting of this signaling exerts superior antitumor effects on c-MET-activated SS. HGF/c-MET expression status is a potential biomarker for identification of SS patients with a worse prognosis who can benefit from c-MET inhibitors.
Title: Functional and therapeutic relevance of hepatocyte growth factor/c-MET signaling in synovial sarcoma.

The following HGF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HGF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25775 NM_000601.5
Latest version!
Homo sapiens hepatocyte growth factor (HGF), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu25798 NM_001010931.2
Latest version!
Homo sapiens hepatocyte growth factor (HGF), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu26016 NM_001010933.2
Latest version!
Homo sapiens hepatocyte growth factor (HGF), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu25775 XM_006715956.2
Latest version!
Homo sapiens hepatocyte growth factor (HGF), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu25798 XM_017012097.1
Latest version!
Homo sapiens hepatocyte growth factor (HGF), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu26016 XM_017012098.1
Latest version!
Homo sapiens hepatocyte growth factor (HGF), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu25927 NM_001010934.2
Latest version!
Homo sapiens hepatocyte growth factor (HGF), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $188.30
$269.00
OHu25877 NM_001010932.2
Latest version!
Homo sapiens hepatocyte growth factor (HGF), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu25877 XM_011516115.2
Latest version!
Homo sapiens hepatocyte growth factor (HGF), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
View Old Accession Versions >>
OHu25775 NM_000601.4 Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu26016 NM_001010933.1 Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu25798 NM_001010931.1 Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25775
Clone ID Related Accession (Same CDS sequence) NM_000601.4 , XM_006715956.2 , NM_000601.5
Accession Version NM_000601.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-15
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform 1 preproprotein
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGCTGAC AATACTATGA ATGACACTGA TGTTCCTTTG
GAAACAACTG AATGCATCCA AGGTCAAGGA GAAGGCTACA GGGGCACTGT CAATACCATT
TGGAATGGAA TTCCATGTCA GCGTTGGGAT TCTCAGTATC CTCACGAGCA TGACATGACT
CCTGAAAATT TCAAGTGCAA GGACCTACGA GAAAATTACT GCCGAAATCC AGATGGGTCT
GAATCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT
CCAAACTGTG ATATGTCACA TGGACAAGAT TGTTATCGTG GGAATGGCAA AAATTATATG
GGCAACTTAT CCCAAACAAG ATCTGGACTA ACATGTTCAA TGTGGGACAA GAACATGGAA
GACTTACATC GTCATATCTT CTGGGAACCA GATGCAAGTA AGCTGAATGA GAATTACTGC
CGAAATCCAG ATGATGATGC TCATGGACCC TGGTGCTACA CGGGAAATCC ACTCATTCCT
TGGGATTATT GCCCTATTTC TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA
GACCATCCCG TAATATCTTG TGCCAAAACG AAACAATTGC GAGTTGTAAA TGGGATTCCA
ACACGAACAA ACATAGGATG GATGGTTAGT TTGAGATACA GAAATAAACA TATCTGCGGA
GGATCATTGA TAAAGGAGAG TTGGGTTCTT ACTGCACGAC AGTGTTTCCC TTCTCGAGAC
TTGAAAGATT ATGAAGCTTG GCTTGGAATT CATGATGTCC ACGGAAGAGG AGATGAGAAA
TGCAAACAGG TTCTCAATGT TTCCCAGCTG GTATATGGCC CTGAAGGATC AGATCTGGTT
TTAATGAAGC TTGCCAGGCC TGCTGTCCTG GATGATTTTG TTAGTACGAT TGATTTACCT
AATTATGGAT GCACAATTCC TGAAAAGACC AGTTGCAGTG TTTATGGCTG GGGCTACACT
GGATTGATCA ACTATGATGG CCTATTACGA GTGGCACATC TCTATATAAT GGGAAATGAG
AAATGCAGCC AGCATCATCG AGGGAAGGTG ACTCTGAATG AGTCTGAAAT ATGTGCTGGG
GCTGAAAAGA TTGGATCAGG ACCATGTGAG GGGGATTATG GTGGCCCACT TGTTTGTGAG
CAACATAAAA TGAGAATGGT TCTTGGTGTC ATTGTTCCTG GTCGTGGATG TGCCATTCCA
AATCGTCCTG GTATTTTTGT CCGAGTAGCA TATTATGCAA AATGGATACA CAAAATTATT
TTAACATATA AGGTACCACA GTCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000592.3
Translation

Target ORF information:

RefSeq Version NM_000601.4
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000601.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGCTGAC AATACTATGA ATGACACTGA TGTTCCTTTG
GAAACAACTG AATGCATCCA AGGTCAAGGA GAAGGCTACA GGGGCACTGT CAATACCATT
TGGAATGGAA TTCCATGTCA GCGTTGGGAT TCTCAGTATC CTCACGAGCA TGACATGACT
CCTGAAAATT TCAAGTGCAA GGACCTACGA GAAAATTACT GCCGAAATCC AGATGGGTCT
GAATCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT
CCAAACTGTG ATATGTCACA TGGACAAGAT TGTTATCGTG GGAATGGCAA AAATTATATG
GGCAACTTAT CCCAAACAAG ATCTGGACTA ACATGTTCAA TGTGGGACAA GAACATGGAA
GACTTACATC GTCATATCTT CTGGGAACCA GATGCAAGTA AGCTGAATGA GAATTACTGC
CGAAATCCAG ATGATGATGC TCATGGACCC TGGTGCTACA CGGGAAATCC ACTCATTCCT
TGGGATTATT GCCCTATTTC TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA
GACCATCCCG TAATATCTTG TGCCAAAACG AAACAATTGC GAGTTGTAAA TGGGATTCCA
ACACGAACAA ACATAGGATG GATGGTTAGT TTGAGATACA GAAATAAACA TATCTGCGGA
GGATCATTGA TAAAGGAGAG TTGGGTTCTT ACTGCACGAC AGTGTTTCCC TTCTCGAGAC
TTGAAAGATT ATGAAGCTTG GCTTGGAATT CATGATGTCC ACGGAAGAGG AGATGAGAAA
TGCAAACAGG TTCTCAATGT TTCCCAGCTG GTATATGGCC CTGAAGGATC AGATCTGGTT
TTAATGAAGC TTGCCAGGCC TGCTGTCCTG GATGATTTTG TTAGTACGAT TGATTTACCT
AATTATGGAT GCACAATTCC TGAAAAGACC AGTTGCAGTG TTTATGGCTG GGGCTACACT
GGATTGATCA ACTATGATGG CCTATTACGA GTGGCACATC TCTATATAAT GGGAAATGAG
AAATGCAGCC AGCATCATCG AGGGAAGGTG ACTCTGAATG AGTCTGAAAT ATGTGCTGGG
GCTGAAAAGA TTGGATCAGG ACCATGTGAG GGGGATTATG GTGGCCCACT TGTTTGTGAG
CAACATAAAA TGAGAATGGT TCTTGGTGTC ATTGTTCCTG GTCGTGGATG TGCCATTCCA
AATCGTCCTG GTATTTTTGT CCGAGTAGCA TATTATGCAA AATGGATACA CAAAATTATT
TTAACATATA AGGTACCACA GTCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25775
Clone ID Related Accession (Same CDS sequence) NM_000601.4 , XM_006715956.2 , NM_000601.5
Accession Version NM_000601.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX495370.1, BU533235.1, M29145.1, M73240.1, AC004960.1 and AV701693.1. This sequence is a reference standard in the RefSeqGene project. On Jan 8, 2016 this sequence version replaced NM_000601.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.93750.1, X16323.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGCTGAC AATACTATGA ATGACACTGA TGTTCCTTTG
GAAACAACTG AATGCATCCA AGGTCAAGGA GAAGGCTACA GGGGCACTGT CAATACCATT
TGGAATGGAA TTCCATGTCA GCGTTGGGAT TCTCAGTATC CTCACGAGCA TGACATGACT
CCTGAAAATT TCAAGTGCAA GGACCTACGA GAAAATTACT GCCGAAATCC AGATGGGTCT
GAATCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT
CCAAACTGTG ATATGTCACA TGGACAAGAT TGTTATCGTG GGAATGGCAA AAATTATATG
GGCAACTTAT CCCAAACAAG ATCTGGACTA ACATGTTCAA TGTGGGACAA GAACATGGAA
GACTTACATC GTCATATCTT CTGGGAACCA GATGCAAGTA AGCTGAATGA GAATTACTGC
CGAAATCCAG ATGATGATGC TCATGGACCC TGGTGCTACA CGGGAAATCC ACTCATTCCT
TGGGATTATT GCCCTATTTC TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA
GACCATCCCG TAATATCTTG TGCCAAAACG AAACAATTGC GAGTTGTAAA TGGGATTCCA
ACACGAACAA ACATAGGATG GATGGTTAGT TTGAGATACA GAAATAAACA TATCTGCGGA
GGATCATTGA TAAAGGAGAG TTGGGTTCTT ACTGCACGAC AGTGTTTCCC TTCTCGAGAC
TTGAAAGATT ATGAAGCTTG GCTTGGAATT CATGATGTCC ACGGAAGAGG AGATGAGAAA
TGCAAACAGG TTCTCAATGT TTCCCAGCTG GTATATGGCC CTGAAGGATC AGATCTGGTT
TTAATGAAGC TTGCCAGGCC TGCTGTCCTG GATGATTTTG TTAGTACGAT TGATTTACCT
AATTATGGAT GCACAATTCC TGAAAAGACC AGTTGCAGTG TTTATGGCTG GGGCTACACT
GGATTGATCA ACTATGATGG CCTATTACGA GTGGCACATC TCTATATAAT GGGAAATGAG
AAATGCAGCC AGCATCATCG AGGGAAGGTG ACTCTGAATG AGTCTGAAAT ATGTGCTGGG
GCTGAAAAGA TTGGATCAGG ACCATGTGAG GGGGATTATG GTGGCCCACT TGTTTGTGAG
CAACATAAAA TGAGAATGGT TCTTGGTGTC ATTGTTCCTG GTCGTGGATG TGCCATTCCA
AATCGTCCTG GTATTTTTGT CCGAGTAGCA TATTATGCAA AATGGATACA CAAAATTATT
TTAACATATA AGGTACCACA GTCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000592.3
CDS228..2414
Misc Feature(1)120..122
Misc Feature(2)321..323
Misc Feature(3)342..593
Misc Feature(4)order(405..407,411..413,444..446)
Misc Feature(5)603..851
Misc Feature(6)636..638
Misc Feature(7)order(681..683,711..713,789..791,795..797,819..821)
Misc Feature(8)855..1097
Misc Feature(9)885..887
Misc Feature(10)order(930..932,960..962,1035..1037,1041..1043,1065..1067)
Misc Feature(11)1137..1379
Misc Feature(12)1167..1169
Misc Feature(13)order(1212..1214,1242..1244,1317..1319,1323..1325,1347..1349)
Misc Feature(14)1389..1637
Misc Feature(15)1425..1427
Misc Feature(16)order(1470..1472,1500..1502,1578..1580,1584..1586,1608..1610)
Misc Feature(17)1497..1499
Misc Feature(18)1497..1499
Misc Feature(19)1707..2375
Misc Feature(20)1707..1709
Misc Feature(21)1707..1709
Misc Feature(22)1707..1709
Misc Feature(23)1710..2384
Misc Feature(24)1710..1712
Misc Feature(25)order(1827..1829,1959..1961,2244..2246)
Misc Feature(26)order(2226..2228,2301..2303,2307..2309)
Exon (1)1..315
Exon (2)316..481
Exon (3)482..594
Exon (4)595..709
Exon (5)710..852
Exon (6)853..973
Exon (7)974..1092
Exon (8)1093..1267
Exon (9)1268..1395
Exon (10)1396..1498
Exon (11)1499..1632
Exon (12)1633..1671
Exon (13)1672..1768
Exon (14)1769..1843
Exon (15)1844..1984
Exon (16)1985..2091
Exon (17)2092..2237
Exon (18)2238..5987
Translation

Target ORF information:

RefSeq Version NM_000601.5
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (HGF), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000601.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGCTGAC AATACTATGA ATGACACTGA TGTTCCTTTG
GAAACAACTG AATGCATCCA AGGTCAAGGA GAAGGCTACA GGGGCACTGT CAATACCATT
TGGAATGGAA TTCCATGTCA GCGTTGGGAT TCTCAGTATC CTCACGAGCA TGACATGACT
CCTGAAAATT TCAAGTGCAA GGACCTACGA GAAAATTACT GCCGAAATCC AGATGGGTCT
GAATCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT
CCAAACTGTG ATATGTCACA TGGACAAGAT TGTTATCGTG GGAATGGCAA AAATTATATG
GGCAACTTAT CCCAAACAAG ATCTGGACTA ACATGTTCAA TGTGGGACAA GAACATGGAA
GACTTACATC GTCATATCTT CTGGGAACCA GATGCAAGTA AGCTGAATGA GAATTACTGC
CGAAATCCAG ATGATGATGC TCATGGACCC TGGTGCTACA CGGGAAATCC ACTCATTCCT
TGGGATTATT GCCCTATTTC TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA
GACCATCCCG TAATATCTTG TGCCAAAACG AAACAATTGC GAGTTGTAAA TGGGATTCCA
ACACGAACAA ACATAGGATG GATGGTTAGT TTGAGATACA GAAATAAACA TATCTGCGGA
GGATCATTGA TAAAGGAGAG TTGGGTTCTT ACTGCACGAC AGTGTTTCCC TTCTCGAGAC
TTGAAAGATT ATGAAGCTTG GCTTGGAATT CATGATGTCC ACGGAAGAGG AGATGAGAAA
TGCAAACAGG TTCTCAATGT TTCCCAGCTG GTATATGGCC CTGAAGGATC AGATCTGGTT
TTAATGAAGC TTGCCAGGCC TGCTGTCCTG GATGATTTTG TTAGTACGAT TGATTTACCT
AATTATGGAT GCACAATTCC TGAAAAGACC AGTTGCAGTG TTTATGGCTG GGGCTACACT
GGATTGATCA ACTATGATGG CCTATTACGA GTGGCACATC TCTATATAAT GGGAAATGAG
AAATGCAGCC AGCATCATCG AGGGAAGGTG ACTCTGAATG AGTCTGAAAT ATGTGCTGGG
GCTGAAAAGA TTGGATCAGG ACCATGTGAG GGGGATTATG GTGGCCCACT TGTTTGTGAG
CAACATAAAA TGAGAATGGT TCTTGGTGTC ATTGTTCCTG GTCGTGGATG TGCCATTCCA
AATCGTCCTG GTATTTTTGT CCGAGTAGCA TATTATGCAA AATGGATACA CAAAATTATT
TTAACATATA AGGTACCACA GTCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25798
Clone ID Related Accession (Same CDS sequence) NM_001010931.2 , XM_017012097.1 , NM_001010931.1
Accession Version NM_001010931.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX495370.1, BU533235.1, L02931.1 and BM971901.1. On Nov 11, 2015 this sequence version replaced NM_001010931.1. Transcript Variant: This variant (2) lacks multiple 3' exons but includes an alternate 3' exon, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus, compared to isoform 1. Isoform 2, also named NK2, has been shown to act as a competitive antagonist to active hepatocyte growth factor for the c-Met receptor. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X57574.1, M77227.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGAGACA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001010931.1
CDS228..1100
Misc Feature(1)120..122
Misc Feature(2)321..323
Misc Feature(3)342..593
Misc Feature(4)order(405..407,411..413,444..446)
Misc Feature(5)603..851
Misc Feature(6)636..638
Misc Feature(7)order(681..683,711..713,789..791,795..797,819..821)
Misc Feature(8)855..1094
Misc Feature(9)885..887
Misc Feature(10)order(930..932,960..962,1035..1037,1041..1043,1065..1067)
Exon (1)1..315
Exon (2)316..481
Exon (3)482..594
Exon (4)595..709
Exon (5)710..852
Exon (6)853..973
Exon (7)974..1092
Exon (8)1093..1351
Translation

Target ORF information:

RefSeq Version NM_001010931.2
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (HGF), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001010931.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGAGACA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25798
Clone ID Related Accession (Same CDS sequence) NM_001010931.2 , XM_017012097.1 , NM_001010931.1
Accession Version NM_001010931.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-15
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform 2 precursor
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGAGACA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001010931.1
Translation

Target ORF information:

RefSeq Version NM_001010931.1
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001010931.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGAGACA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26016
Clone ID Related Accession (Same CDS sequence) NM_001010933.1 , NM_001010933.2 , XM_017012098.1
Accession Version NM_001010933.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 858bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-15
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform 4 precursor
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG AGACATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001010933.1
Translation

Target ORF information:

RefSeq Version NM_001010933.1
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (hepapoietin A; scatter factor) (HGF), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001010933.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG AGACATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26016
Clone ID Related Accession (Same CDS sequence) NM_001010933.1 , NM_001010933.2 , XM_017012098.1
Accession Version NM_001010933.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 858bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX495370.1, BU533235.1, BC022308.1, L02931.1 and BM971901.1. On Nov 11, 2015 this sequence version replaced NM_001010933.1. Transcript Variant: This variant (4) lacks an internal in-frame segment and multiple 3' exons but includes an alternate 3' exon, compared to variant 1. The resulting protein (isoform 4) lacks an internal segment and has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022308.1, AL549164.3 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG AGACATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001010933.1
CDS228..1085
Misc Feature(1)120..122
Misc Feature(2)321..323
Misc Feature(3)342..593
Misc Feature(4)order(405..407,411..413,444..446)
Misc Feature(5)603..836
Misc Feature(6)636..638
Misc Feature(7)order(681..683,711..713,774..776,780..782,804..806)
Misc Feature(8)840..1079
Misc Feature(9)870..872
Misc Feature(10)order(915..917,945..947,1020..1022,1026..1028,1050..1052)
Exon (1)1..315
Exon (2)316..481
Exon (3)482..594
Exon (4)595..709
Exon (5)710..837
Exon (6)838..958
Exon (7)959..1077
Exon (8)1078..1336
Translation

Target ORF information:

RefSeq Version NM_001010933.2
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (HGF), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001010933.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG AGACATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25775
Clone ID Related Accession (Same CDS sequence) NM_000601.4 , XM_006715956.2 , NM_000601.5
Accession Version XM_006715956.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006715956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGCTGAC AATACTATGA ATGACACTGA TGTTCCTTTG
GAAACAACTG AATGCATCCA AGGTCAAGGA GAAGGCTACA GGGGCACTGT CAATACCATT
TGGAATGGAA TTCCATGTCA GCGTTGGGAT TCTCAGTATC CTCACGAGCA TGACATGACT
CCTGAAAATT TCAAGTGCAA GGACCTACGA GAAAATTACT GCCGAAATCC AGATGGGTCT
GAATCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT
CCAAACTGTG ATATGTCACA TGGACAAGAT TGTTATCGTG GGAATGGCAA AAATTATATG
GGCAACTTAT CCCAAACAAG ATCTGGACTA ACATGTTCAA TGTGGGACAA GAACATGGAA
GACTTACATC GTCATATCTT CTGGGAACCA GATGCAAGTA AGCTGAATGA GAATTACTGC
CGAAATCCAG ATGATGATGC TCATGGACCC TGGTGCTACA CGGGAAATCC ACTCATTCCT
TGGGATTATT GCCCTATTTC TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA
GACCATCCCG TAATATCTTG TGCCAAAACG AAACAATTGC GAGTTGTAAA TGGGATTCCA
ACACGAACAA ACATAGGATG GATGGTTAGT TTGAGATACA GAAATAAACA TATCTGCGGA
GGATCATTGA TAAAGGAGAG TTGGGTTCTT ACTGCACGAC AGTGTTTCCC TTCTCGAGAC
TTGAAAGATT ATGAAGCTTG GCTTGGAATT CATGATGTCC ACGGAAGAGG AGATGAGAAA
TGCAAACAGG TTCTCAATGT TTCCCAGCTG GTATATGGCC CTGAAGGATC AGATCTGGTT
TTAATGAAGC TTGCCAGGCC TGCTGTCCTG GATGATTTTG TTAGTACGAT TGATTTACCT
AATTATGGAT GCACAATTCC TGAAAAGACC AGTTGCAGTG TTTATGGCTG GGGCTACACT
GGATTGATCA ACTATGATGG CCTATTACGA GTGGCACATC TCTATATAAT GGGAAATGAG
AAATGCAGCC AGCATCATCG AGGGAAGGTG ACTCTGAATG AGTCTGAAAT ATGTGCTGGG
GCTGAAAAGA TTGGATCAGG ACCATGTGAG GGGGATTATG GTGGCCCACT TGTTTGTGAG
CAACATAAAA TGAGAATGGT TCTTGGTGTC ATTGTTCCTG GTCGTGGATG TGCCATTCCA
AATCGTCCTG GTATTTTTGT CCGAGTAGCA TATTATGCAA AATGGATACA CAAAATTATT
TTAACATATA AGGTACCACA GTCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006716019.1
CDS191..2377
Misc Feature(1)305..556
Misc Feature(2)order(368..370,374..376,407..409)
Misc Feature(3)566..814
Misc Feature(4)599..601
Misc Feature(5)order(644..646,674..676,752..754,758..760,782..784)
Misc Feature(6)818..1060
Misc Feature(7)848..850
Misc Feature(8)order(893..895,923..925,998..1000,1004..1006,1028..1030)
Misc Feature(9)1100..1342
Misc Feature(10)1130..1132
Misc Feature(11)order(1175..1177,1205..1207,1280..1282,1286..1288,1310..1312)
Misc Feature(12)1352..1600
Misc Feature(13)1388..1390
Misc Feature(14)order(1433..1435,1463..1465,1541..1543,1547..1549,1571..1573)
Misc Feature(15)1670..2338
Misc Feature(16)1673..2347
Misc Feature(17)1673..1675
Misc Feature(18)order(1790..1792,1922..1924,2207..2209)
Misc Feature(19)order(2189..2191,2264..2266,2270..2272)
Translation

Target ORF information:

RefSeq Version XM_006715956.2
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (HGF), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006715956.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGCTGAC AATACTATGA ATGACACTGA TGTTCCTTTG
GAAACAACTG AATGCATCCA AGGTCAAGGA GAAGGCTACA GGGGCACTGT CAATACCATT
TGGAATGGAA TTCCATGTCA GCGTTGGGAT TCTCAGTATC CTCACGAGCA TGACATGACT
CCTGAAAATT TCAAGTGCAA GGACCTACGA GAAAATTACT GCCGAAATCC AGATGGGTCT
GAATCACCCT GGTGTTTTAC CACTGATCCA AACATCCGAG TTGGCTACTG CTCCCAAATT
CCAAACTGTG ATATGTCACA TGGACAAGAT TGTTATCGTG GGAATGGCAA AAATTATATG
GGCAACTTAT CCCAAACAAG ATCTGGACTA ACATGTTCAA TGTGGGACAA GAACATGGAA
GACTTACATC GTCATATCTT CTGGGAACCA GATGCAAGTA AGCTGAATGA GAATTACTGC
CGAAATCCAG ATGATGATGC TCATGGACCC TGGTGCTACA CGGGAAATCC ACTCATTCCT
TGGGATTATT GCCCTATTTC TCGTTGTGAA GGTGATACCA CACCTACAAT AGTCAATTTA
GACCATCCCG TAATATCTTG TGCCAAAACG AAACAATTGC GAGTTGTAAA TGGGATTCCA
ACACGAACAA ACATAGGATG GATGGTTAGT TTGAGATACA GAAATAAACA TATCTGCGGA
GGATCATTGA TAAAGGAGAG TTGGGTTCTT ACTGCACGAC AGTGTTTCCC TTCTCGAGAC
TTGAAAGATT ATGAAGCTTG GCTTGGAATT CATGATGTCC ACGGAAGAGG AGATGAGAAA
TGCAAACAGG TTCTCAATGT TTCCCAGCTG GTATATGGCC CTGAAGGATC AGATCTGGTT
TTAATGAAGC TTGCCAGGCC TGCTGTCCTG GATGATTTTG TTAGTACGAT TGATTTACCT
AATTATGGAT GCACAATTCC TGAAAAGACC AGTTGCAGTG TTTATGGCTG GGGCTACACT
GGATTGATCA ACTATGATGG CCTATTACGA GTGGCACATC TCTATATAAT GGGAAATGAG
AAATGCAGCC AGCATCATCG AGGGAAGGTG ACTCTGAATG AGTCTGAAAT ATGTGCTGGG
GCTGAAAAGA TTGGATCAGG ACCATGTGAG GGGGATTATG GTGGCCCACT TGTTTGTGAG
CAACATAAAA TGAGAATGGT TCTTGGTGTC ATTGTTCCTG GTCGTGGATG TGCCATTCCA
AATCGTCCTG GTATTTTTGT CCGAGTAGCA TATTATGCAA AATGGATACA CAAAATTATT
TTAACATATA AGGTACCACA GTCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25798
Clone ID Related Accession (Same CDS sequence) NM_001010931.2 , XM_017012097.1 , NM_001010931.1
Accession Version XM_017012097.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGAGACA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867586.1
CDS191..1063
Misc Feature(1)305..556
Misc Feature(2)order(368..370,374..376,407..409)
Misc Feature(3)566..814
Misc Feature(4)599..601
Misc Feature(5)order(644..646,674..676,752..754,758..760,782..784)
Misc Feature(6)818..1057
Misc Feature(7)848..850
Misc Feature(8)order(893..895,923..925,998..1000,1004..1006,1028..1030)
Translation

Target ORF information:

RefSeq Version XM_017012097.1
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (HGF), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012097.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA
GGTCTCATGG ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA
CACCGGCACA AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC
CGCAATCCCG ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG
GAGTACTGTG CAATTAAAAC ATGCGAGACA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26016
Clone ID Related Accession (Same CDS sequence) NM_001010933.1 , NM_001010933.2 , XM_017012098.1
Accession Version XM_017012098.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 858bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG AGACATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867587.1
CDS191..1048
Misc Feature(1)305..556
Misc Feature(2)order(368..370,374..376,407..409)
Misc Feature(3)566..799
Misc Feature(4)599..601
Misc Feature(5)order(644..646,674..676,737..739,743..745,767..769)
Misc Feature(6)803..1042
Misc Feature(7)833..835
Misc Feature(8)order(878..880,908..910,983..985,989..991,1013..1015)
Translation

Target ORF information:

RefSeq Version XM_017012098.1
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (HGF), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012098.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG AGACATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25927
Clone ID Related Accession (Same CDS sequence) NM_001010934.2
Accession Version NM_001010934.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 633bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform 5 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX495370.1, BU533235.1, U46010.1 and BC063485.1. On Nov 11, 2015 this sequence version replaced NM_001010934.1. Transcript Variant: This variant (5) lacks multiple 3' exons but has an alternate 3' segment, compared to variant 1. The resulting protein (isoform 5) has a distinct and shorter C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063485.1, AB208900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGGTAAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001010934.1
CDS228..860
Misc Feature(1)120..122
Misc Feature(2)321..323
Misc Feature(3)342..593
Misc Feature(4)order(405..407,411..413,444..446)
Misc Feature(5)603..851
Misc Feature(6)636..638
Misc Feature(7)order(681..683,711..713,789..791,795..797,819..821)
Exon (1)1..315
Exon (2)316..481
Exon (3)482..594
Exon (4)595..709
Exon (5)710..2072
Translation

Target ORF information:

RefSeq Version NM_001010934.2
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (HGF), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001010934.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTTTTTGC CTTCGAGCTA TCGGGGTAAA GACCTACAGG AAAACTACTG TCGAAATCCT
CGAGGGGAAG AAGGGGGACC CTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC
TGTGACATTC CTCAGTGTTC AGAAGGTAAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25877
Clone ID Related Accession (Same CDS sequence) NM_001010932.2 , XM_011516115.2
Accession Version NM_001010932.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX495370.1, BU533235.1, M73240.1, AC004960.1 and AV701693.1. On Jan 8, 2016 this sequence version replaced NM_001010932.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (3) lacks a portion of the alpha chain and is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M73240.1, AK313694.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG CTGACAATAC TATGAATGAC ACTGATGTTC CTTTGGAAAC AACTGAATGC
ATCCAAGGTC AAGGAGAAGG CTACAGGGGC ACTGTCAATA CCATTTGGAA TGGAATTCCA
TGTCAGCGTT GGGATTCTCA GTATCCTCAC GAGCATGACA TGACTCCTGA AAATTTCAAG
TGCAAGGACC TACGAGAAAA TTACTGCCGA AATCCAGATG GGTCTGAATC ACCCTGGTGT
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA CTGTGATATG
TCACATGGAC AAGATTGTTA TCGTGGGAAT GGCAAAAATT ATATGGGCAA CTTATCCCAA
ACAAGATCTG GACTAACATG TTCAATGTGG GACAAGAACA TGGAAGACTT ACATCGTCAT
ATCTTCTGGG AACCAGATGC AAGTAAGCTG AATGAGAATT ACTGCCGAAA TCCAGATGAT
GATGCTCATG GACCCTGGTG CTACACGGGA AATCCACTCA TTCCTTGGGA TTATTGCCCT
ATTTCTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCCGTAATA
TCTTGTGCCA AAACGAAACA ATTGCGAGTT GTAAATGGGA TTCCAACACG AACAAACATA
GGATGGATGG TTAGTTTGAG ATACAGAAAT AAACATATCT GCGGAGGATC ATTGATAAAG
GAGAGTTGGG TTCTTACTGC ACGACAGTGT TTCCCTTCTC GAGACTTGAA AGATTATGAA
GCTTGGCTTG GAATTCATGA TGTCCACGGA AGAGGAGATG AGAAATGCAA ACAGGTTCTC
AATGTTTCCC AGCTGGTATA TGGCCCTGAA GGATCAGATC TGGTTTTAAT GAAGCTTGCC
AGGCCTGCTG TCCTGGATGA TTTTGTTAGT ACGATTGATT TACCTAATTA TGGATGCACA
ATTCCTGAAA AGACCAGTTG CAGTGTTTAT GGCTGGGGCT ACACTGGATT GATCAACTAT
GATGGCCTAT TACGAGTGGC ACATCTCTAT ATAATGGGAA ATGAGAAATG CAGCCAGCAT
CATCGAGGGA AGGTGACTCT GAATGAGTCT GAAATATGTG CTGGGGCTGA AAAGATTGGA
TCAGGACCAT GTGAGGGGGA TTATGGTGGC CCACTTGTTT GTGAGCAACA TAAAATGAGA
ATGGTTCTTG GTGTCATTGT TCCTGGTCGT GGATGTGCCA TTCCAAATCG TCCTGGTATT
TTTGTCCGAG TAGCATATTA TGCAAAATGG ATACACAAAA TTATTTTAAC ATATAAGGTA
CCACAGTCAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001010932.1
CDS228..2399
Misc Feature(1)120..122
Misc Feature(2)321..323
Misc Feature(3)342..593
Misc Feature(4)order(405..407,411..413,444..446)
Misc Feature(5)603..836
Misc Feature(6)636..638
Misc Feature(7)order(681..683,711..713,774..776,780..782,804..806)
Misc Feature(8)840..1082
Misc Feature(9)870..872
Misc Feature(10)order(915..917,945..947,1020..1022,1026..1028,1050..1052)
Misc Feature(11)1122..1364
Misc Feature(12)1152..1154
Misc Feature(13)order(1197..1199,1227..1229,1302..1304,1308..1310,1332..1334)
Misc Feature(14)1374..1622
Misc Feature(15)1410..1412
Misc Feature(16)order(1455..1457,1485..1487,1563..1565,1569..1571,1593..1595)
Misc Feature(17)1482..1484
Misc Feature(18)1482..1484
Misc Feature(19)1692..2360
Misc Feature(20)1692..1694
Misc Feature(21)1692..1694
Misc Feature(22)1692..1694
Misc Feature(23)1695..2369
Misc Feature(24)1695..1697
Misc Feature(25)order(1812..1814,1944..1946,2229..2231)
Misc Feature(26)order(2211..2213,2286..2288,2292..2294)
Exon (1)1..315
Exon (2)316..481
Exon (3)482..594
Exon (4)595..709
Exon (5)710..837
Exon (6)838..958
Exon (7)959..1077
Exon (8)1078..1252
Exon (9)1253..1380
Exon (10)1381..1483
Exon (11)1484..1617
Exon (12)1618..1656
Exon (13)1657..1753
Exon (14)1754..1828
Exon (15)1829..1969
Exon (16)1970..2076
Exon (17)2077..2222
Exon (18)2223..5972
Translation

Target ORF information:

RefSeq Version NM_001010932.2
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (HGF), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001010932.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG CTGACAATAC TATGAATGAC ACTGATGTTC CTTTGGAAAC AACTGAATGC
ATCCAAGGTC AAGGAGAAGG CTACAGGGGC ACTGTCAATA CCATTTGGAA TGGAATTCCA
TGTCAGCGTT GGGATTCTCA GTATCCTCAC GAGCATGACA TGACTCCTGA AAATTTCAAG
TGCAAGGACC TACGAGAAAA TTACTGCCGA AATCCAGATG GGTCTGAATC ACCCTGGTGT
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA CTGTGATATG
TCACATGGAC AAGATTGTTA TCGTGGGAAT GGCAAAAATT ATATGGGCAA CTTATCCCAA
ACAAGATCTG GACTAACATG TTCAATGTGG GACAAGAACA TGGAAGACTT ACATCGTCAT
ATCTTCTGGG AACCAGATGC AAGTAAGCTG AATGAGAATT ACTGCCGAAA TCCAGATGAT
GATGCTCATG GACCCTGGTG CTACACGGGA AATCCACTCA TTCCTTGGGA TTATTGCCCT
ATTTCTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCCGTAATA
TCTTGTGCCA AAACGAAACA ATTGCGAGTT GTAAATGGGA TTCCAACACG AACAAACATA
GGATGGATGG TTAGTTTGAG ATACAGAAAT AAACATATCT GCGGAGGATC ATTGATAAAG
GAGAGTTGGG TTCTTACTGC ACGACAGTGT TTCCCTTCTC GAGACTTGAA AGATTATGAA
GCTTGGCTTG GAATTCATGA TGTCCACGGA AGAGGAGATG AGAAATGCAA ACAGGTTCTC
AATGTTTCCC AGCTGGTATA TGGCCCTGAA GGATCAGATC TGGTTTTAAT GAAGCTTGCC
AGGCCTGCTG TCCTGGATGA TTTTGTTAGT ACGATTGATT TACCTAATTA TGGATGCACA
ATTCCTGAAA AGACCAGTTG CAGTGTTTAT GGCTGGGGCT ACACTGGATT GATCAACTAT
GATGGCCTAT TACGAGTGGC ACATCTCTAT ATAATGGGAA ATGAGAAATG CAGCCAGCAT
CATCGAGGGA AGGTGACTCT GAATGAGTCT GAAATATGTG CTGGGGCTGA AAAGATTGGA
TCAGGACCAT GTGAGGGGGA TTATGGTGGC CCACTTGTTT GTGAGCAACA TAAAATGAGA
ATGGTTCTTG GTGTCATTGT TCCTGGTCGT GGATGTGCCA TTCCAAATCG TCCTGGTATT
TTTGTCCGAG TAGCATATTA TGCAAAATGG ATACACAAAA TTATTTTAAC ATATAAGGTA
CCACAGTCAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25877
Clone ID Related Accession (Same CDS sequence) NM_001010932.2 , XM_011516115.2
Accession Version XM_011516115.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hepatocyte growth factor isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG CTGACAATAC TATGAATGAC ACTGATGTTC CTTTGGAAAC AACTGAATGC
ATCCAAGGTC AAGGAGAAGG CTACAGGGGC ACTGTCAATA CCATTTGGAA TGGAATTCCA
TGTCAGCGTT GGGATTCTCA GTATCCTCAC GAGCATGACA TGACTCCTGA AAATTTCAAG
TGCAAGGACC TACGAGAAAA TTACTGCCGA AATCCAGATG GGTCTGAATC ACCCTGGTGT
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA CTGTGATATG
TCACATGGAC AAGATTGTTA TCGTGGGAAT GGCAAAAATT ATATGGGCAA CTTATCCCAA
ACAAGATCTG GACTAACATG TTCAATGTGG GACAAGAACA TGGAAGACTT ACATCGTCAT
ATCTTCTGGG AACCAGATGC AAGTAAGCTG AATGAGAATT ACTGCCGAAA TCCAGATGAT
GATGCTCATG GACCCTGGTG CTACACGGGA AATCCACTCA TTCCTTGGGA TTATTGCCCT
ATTTCTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCCGTAATA
TCTTGTGCCA AAACGAAACA ATTGCGAGTT GTAAATGGGA TTCCAACACG AACAAACATA
GGATGGATGG TTAGTTTGAG ATACAGAAAT AAACATATCT GCGGAGGATC ATTGATAAAG
GAGAGTTGGG TTCTTACTGC ACGACAGTGT TTCCCTTCTC GAGACTTGAA AGATTATGAA
GCTTGGCTTG GAATTCATGA TGTCCACGGA AGAGGAGATG AGAAATGCAA ACAGGTTCTC
AATGTTTCCC AGCTGGTATA TGGCCCTGAA GGATCAGATC TGGTTTTAAT GAAGCTTGCC
AGGCCTGCTG TCCTGGATGA TTTTGTTAGT ACGATTGATT TACCTAATTA TGGATGCACA
ATTCCTGAAA AGACCAGTTG CAGTGTTTAT GGCTGGGGCT ACACTGGATT GATCAACTAT
GATGGCCTAT TACGAGTGGC ACATCTCTAT ATAATGGGAA ATGAGAAATG CAGCCAGCAT
CATCGAGGGA AGGTGACTCT GAATGAGTCT GAAATATGTG CTGGGGCTGA AAAGATTGGA
TCAGGACCAT GTGAGGGGGA TTATGGTGGC CCACTTGTTT GTGAGCAACA TAAAATGAGA
ATGGTTCTTG GTGTCATTGT TCCTGGTCGT GGATGTGCCA TTCCAAATCG TCCTGGTATT
TTTGTCCGAG TAGCATATTA TGCAAAATGG ATACACAAAA TTATTTTAAC ATATAAGGTA
CCACAGTCAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514417.1
CDS191..2362
Misc Feature(1)305..556
Misc Feature(2)order(368..370,374..376,407..409)
Misc Feature(3)566..799
Misc Feature(4)599..601
Misc Feature(5)order(644..646,674..676,737..739,743..745,767..769)
Misc Feature(6)803..1045
Misc Feature(7)833..835
Misc Feature(8)order(878..880,908..910,983..985,989..991,1013..1015)
Misc Feature(9)1085..1327
Misc Feature(10)1115..1117
Misc Feature(11)order(1160..1162,1190..1192,1265..1267,1271..1273,1295..1297)
Misc Feature(12)1337..1585
Misc Feature(13)1373..1375
Misc Feature(14)order(1418..1420,1448..1450,1526..1528,1532..1534,1556..1558)
Misc Feature(15)1655..2323
Misc Feature(16)1658..2332
Misc Feature(17)1658..1660
Misc Feature(18)order(1775..1777,1907..1909,2192..2194)
Misc Feature(19)order(2174..2176,2249..2251,2255..2257)
Translation

Target ORF information:

RefSeq Version XM_011516115.2
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte growth factor (HGF), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516115.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTGGGTGA CCAAACTCCT GCCAGCCCTG CTGCTGCAGC ATGTCCTCCT GCATCTCCTC 
CTGCTCCCCA TCGCCATCCC CTATGCAGAG GGACAAAGGA AAAGAAGAAA TACAATTCAT
GAATTCAAAA AATCAGCAAA GACTACCCTA ATCAAAATAG ATCCAGCACT GAAGATAAAA
ACCAAAAAAG TGAATACTGC AGACCAATGT GCTAATAGAT GTACTAGGAA TAAAGGACTT
CCATTCACTT GCAAGGCTTT TGTTTTTGAT AAAGCAAGAA AACAATGCCT CTGGTTCCCC
TTCAATAGCA TGTCAAGTGG AGTGAAAAAA GAATTTGGCC ATGAATTTGA CCTCTATGAA
AACAAAGACT ACATTAGAAA CTGCATCATT GGTAAAGGAC GCAGCTACAA GGGAACAGTA
TCTATCACTA AGAGTGGCAT CAAATGTCAG CCCTGGAGTT CCATGATACC ACACGAACAC
AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA ATCCTCGAGG GGAAGAAGGG
GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG AAGTCTGTGA CATTCCTCAG
TGTTCAGAAG TTGAATGCAT GACCTGCAAT GGGGAGAGTT ATCGAGGTCT CATGGATCAT
ACAGAATCAG GCAAGATTTG TCAGCGCTGG GATCATCAGA CACCACACCG GCACAAATTC
TTGCCTGAAA GATATCCCGA CAAGGGCTTT GATGATAATT ATTGCCGCAA TCCCGATGGC
CAGCCGAGGC CATGGTGCTA TACTCTTGAC CCTCACACCC GCTGGGAGTA CTGTGCAATT
AAAACATGCG CTGACAATAC TATGAATGAC ACTGATGTTC CTTTGGAAAC AACTGAATGC
ATCCAAGGTC AAGGAGAAGG CTACAGGGGC ACTGTCAATA CCATTTGGAA TGGAATTCCA
TGTCAGCGTT GGGATTCTCA GTATCCTCAC GAGCATGACA TGACTCCTGA AAATTTCAAG
TGCAAGGACC TACGAGAAAA TTACTGCCGA AATCCAGATG GGTCTGAATC ACCCTGGTGT
TTTACCACTG ATCCAAACAT CCGAGTTGGC TACTGCTCCC AAATTCCAAA CTGTGATATG
TCACATGGAC AAGATTGTTA TCGTGGGAAT GGCAAAAATT ATATGGGCAA CTTATCCCAA
ACAAGATCTG GACTAACATG TTCAATGTGG GACAAGAACA TGGAAGACTT ACATCGTCAT
ATCTTCTGGG AACCAGATGC AAGTAAGCTG AATGAGAATT ACTGCCGAAA TCCAGATGAT
GATGCTCATG GACCCTGGTG CTACACGGGA AATCCACTCA TTCCTTGGGA TTATTGCCCT
ATTTCTCGTT GTGAAGGTGA TACCACACCT ACAATAGTCA ATTTAGACCA TCCCGTAATA
TCTTGTGCCA AAACGAAACA ATTGCGAGTT GTAAATGGGA TTCCAACACG AACAAACATA
GGATGGATGG TTAGTTTGAG ATACAGAAAT AAACATATCT GCGGAGGATC ATTGATAAAG
GAGAGTTGGG TTCTTACTGC ACGACAGTGT TTCCCTTCTC GAGACTTGAA AGATTATGAA
GCTTGGCTTG GAATTCATGA TGTCCACGGA AGAGGAGATG AGAAATGCAA ACAGGTTCTC
AATGTTTCCC AGCTGGTATA TGGCCCTGAA GGATCAGATC TGGTTTTAAT GAAGCTTGCC
AGGCCTGCTG TCCTGGATGA TTTTGTTAGT ACGATTGATT TACCTAATTA TGGATGCACA
ATTCCTGAAA AGACCAGTTG CAGTGTTTAT GGCTGGGGCT ACACTGGATT GATCAACTAT
GATGGCCTAT TACGAGTGGC ACATCTCTAT ATAATGGGAA ATGAGAAATG CAGCCAGCAT
CATCGAGGGA AGGTGACTCT GAATGAGTCT GAAATATGTG CTGGGGCTGA AAAGATTGGA
TCAGGACCAT GTGAGGGGGA TTATGGTGGC CCACTTGTTT GTGAGCAACA TAAAATGAGA
ATGGTTCTTG GTGTCATTGT TCCTGGTCGT GGATGTGCCA TTCCAAATCG TCCTGGTATT
TTTGTCCGAG TAGCATATTA TGCAAAATGG ATACACAAAA TTATTTTAAC ATATAAGGTA
CCACAGTCAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Increased HGF Expression Induces Resistance to c-MET Tyrosine Kinase Inhibitors in Gastric Cancer.
Anticancer research37(3)1127-1138(2017 03)
Ahn SY,Kim J,Kim MA,Choi J,Kim WH


book

Dual inhibition using cabozantinib overcomes HGF/MET signaling mediated resistance to pan-VEGFR inhibition in orthotopic and metastatic neuroblastoma tumors.
International journal of oncology50(1)203-211(2017 Jan)
Daudigeos-Dubus E,Le Dret L,Bawa O,Opolon P,Vievard A,Villa I,Bosq J,Vassal G,Geoerger B


book

Plasma hepatocyte growth factor is a novel marker of AL cardiac amyloidosis.
Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis23(4)242-248(2016 Dec)
Swiger KJ,Friedman EA,Brittain EL,Tomasek KA,Huang S,Su YR,Sawyer DB,Lenihan DJ


book

Varying effects of EGF, HGF and TGFβ on formation of invadopodia and invasiveness of melanoma cell lines of different origin.
European journal of histochemistry : EJH60(4)2728(2016 Dec)
Makowiecka A,Simiczyjew A,Nowak D,Mazur AJ


book

Hepatocyte growth factor twenty years on: Much more than a growth factor.
Journal of gastroenterology and hepatology26 Suppl 1188-202(2011 Jan)
Nakamura T,Sakai K,Nakamura T,Matsumoto K


 
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