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HK1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol HK1
Entrez Gene ID 3098
Full Name hexokinase 1
Synonyms HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1
General protein information
Preferred Names
hexokinase-1
Names
hexokinase-1
HK I
glycolytic enzyme
hexokinase type I
brain form hexokinase
NP_000179.2
NP_277031.1
NP_277032.1
NP_277033.1
NP_277035.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q22

Summary Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]. lac of sum
Disorder MIM:

142600

Disorder Html: Hemolytic anemia due to hexokinase deficiency, 235700 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005269735 XP_005269792 hexokinase-1 isoform X3
XM_005269736 XP_005269793 hexokinase-1 isoform X2
XM_011539732 XP_011538034 hexokinase-1 isoform X1
XM_011539733 XP_011538035 hexokinase-1 isoform X4
XM_011539734 XP_011538036 hexokinase-1 isoform X5
XM_005269737 XP_005269794 hexokinase-1 isoform X6
NM_033496 NP_277031 hexokinase-1 isoform HKI-R
NM_033500 NP_277035 hexokinase-1 isoform HKI-td
NM_033498 NP_277033 hexokinase-1 isoform HKI-ta/tb
NM_033497 NP_277032 hexokinase-1 isoform HKI-ta/tb
NM_000188 NP_000179 hexokinase-1 isoform HKI



Homo sapiens (human) HK1 NP_000179.2
Pan troglodytes (chimpanzee) HK1 XP_001169264.2
Macaca mulatta (Rhesus monkey) HK1 XP_001110396.2
Canis lupus familiaris (dog) HK1 XP_536376.3
Bos taurus (cattle) HK1 NP_001012686.1
Mus musculus (house mouse) Hk1 NP_001139572.1
Rattus norvegicus (Norway rat) Hk1 NP_036866.1
Gallus gallus (chicken) HK1 NP_989432.1
Danio rerio (zebrafish) hk1 NP_998417.1
Drosophila melanogaster (fruit fly) Hex-t2 NP_733151.2
Drosophila melanogaster (fruit fly) Hex-C NP_524674.1
Drosophila melanogaster (fruit fly) Hex-A NP_524848.1
Arabidopsis thaliana (thale cress) HXK2 NP_179576.1
Arabidopsis thaliana (thale cress) HXK1 NP_194642.1
Xenopus (Silurana) tropicalis (western clawed frog) hk1 NP_001096201.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Free ORF Clone
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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu44255 XM_005269735 PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $475.30
$679.00
OHu09664 XM_005269736 PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $475.30
$679.00
OHu19393 XM_011539732 PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu58217 XM_011539733 PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu58218 XM_011539734 PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu44256 XM_005269737 PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $475.30
$679.00
OHu09550 NM_033496 Homo sapiens hexokinase 1 (HK1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $475.30
$679.00
OHu19393 NM_033500 Homo sapiens hexokinase 1 (HK1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $475.30
$679.00
OHu09664 NM_033498 Homo sapiens hexokinase 1 (HK1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $475.30
$679.00
OHu09664 NM_033497 Homo sapiens hexokinase 1 (HK1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $475.30
$679.00
OHu10118 NM_000188 Homo sapiens hexokinase 1 (HK1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu44255
Clone ID Related Accession (Same CDS sequence) XM_005269735
Accession Version XM_005269735.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530393495. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGGGACAG GCCACGACCT GTGGCCCAAG TCCCGCCCAG AGGGAGTGCG CCCTGAGGGA 
GAGCCACCGG AGCTGGTGAC TCGGGGGCGG GTGCTCTGGG GTGTCGCTTT GGGTTCAGCA
ATACAAATCT GCCAGCTGGA GATTAGGCGG GGCACGCCGG GGAGAGGTGA TGCACTGCCG
CTGAAGACCC AGATTGACAA GTATCTCTAT GCCATGCGGC TCTCCGATGA AACTCTCATA
GATATCATGA CTCGCTTCAG GAAGGAGATG AAGAATGGCC TCTCCCGGGA TTTTAATCCA
ACAGCCACAG TCAAGATGTT GCCAACATTC GTAAGGTCCA TTCCTGATGG CTCTGAAAAG
GGAGATTTCA TTGCCCTGGA TCTTGGTGGG TCTTCCTTTC GAATTCTGCG GGTGCAAGTG
AATCATGAGA AAAACCAGAA TGTTCACATG GAGTCCGAGG TTTATGACAC CCCAGAGAAC
ATCGTGCACG GCAGTGGAAG CCAGCTTTTT GATCATGTTG CTGAGTGCCT GGGAGATTTC
ATGGAGAAAA GGAAGATCAA GGACAAGAAG TTACCTGTGG GATTCACGTT TTCTTTTCCT
TGCCAACAAT CCAAAATAGA TGAGGCCATC CTGATCACCT GGACAAAGCG ATTTAAAGCG
AGCGGAGTGG AAGGAGCAGA TGTGGTCAAA CTGCTTAACA AAGCCATCAA AAAGCGAGGG
GACTATGATG CCAACATCGT AGCTGTGGTG AATGACACAG TGGGCACCAT GATGACCTGT
GGCTATGACG ACCAGCACTG TGAAGTCGGC CTGATCATCG GCACTGGCAC CAATGCTTGC
TACATGGAGG AACTGAGGCA CATTGATCTG GTGGAAGGAG ACGAGGGGAG GATGTGTATC
AATACAGAAT GGGGAGCCTT TGGAGACGAT GGATCATTAG AAGACATCCG GACAGAGTTT
GACAGGGAGA TAGACCGGGG ATCCCTCAAC CCTGGAAAAC AGCTGTTTGA GAAGATGGTC
AGTGGCATGT ACTTGGGAGA GCTGGTTCGA CTGATCCTAG TCAAGATGGC CAAGGAGGGC
CTCTTATTTG AAGGGCGGAT CACCCCGGAG CTGCTCACCC GAGGGAAGTT TAACACCAGT
GATGTGTCAG CCATCGAAAA GAATAAGGAA GGCCTCCACA ATGCCAAAGA AATCCTGACC
CGCCTGGGAG TGGAGCCGTC CGATGATGAC TGTGTCTCAG TCCAGCACGT TTGCACCATT
GTCTCATTTC GCTCAGCCAA CTTGGTGGCT GCCACACTGG GCGCCATCTT GAACCGCCTG
CGTGATAACA AGGGCACACC CAGGCTGCGG ACCACGGTTG GTGTCGACGG ATCTCTTTAC
AAGACGCACC CACAGTATTC CCGGCGTTTC CACAAGACTC TAAGGCGCTT GGTGCCAGAC
TCCGATGTGC GCTTCCTCCT CTCGGAGAGT GGCAGCGGCA AGGGGGCTGC CATGGTGACG
GCGGTGGCCT ACCGCTTGGC CGAGCAGCAC CGGCAGATAG AGGAGACCCT GGCTCATTTC
CACCTCACCA AGGACATGCT GCTGGAGGTG AAGAAGAGGA TGCGGGCCGA GATGGAGCTG
GGGCTGAGGA AGCAGACGCA CAACAATGCC GTGGTTAAGA TGCTGCCCTC CTTCGTCCGG
AGAACTCCCG ACGGGACCGA GAATGGTGAC TTCTTGGCCC TGGATCTTGG AGGAACCAAT
TTCCGTGTGC TGCTGGTGAA AATCCGTAGT GGGAAAAAGA GAACGGTGGA AATGCACAAC
AAGATCTACG CCATTCCTAT TGAAATCATG CAGGGCACTG GGGAAGAGCT GTTTGATCAC
ATTGTCTCCT GCATCTCTGA CTTCTTGGAC TACATGGGGA TCAAAGGCCC CAGGATGCCT
CTGGGCTTCA CGTTCTCATT TCCCTGCCAG CAGACGAGTC TGGACGCGGG AATCTTGATC
ACGTGGACAA AGGGTTTTAA GGCAACAGAC TGCGTGGGCC ACGATGTAGT CACCTTACTA
AGGGATGCGA TAAAAAGGAG AGAGGAATTT GACCTGGACG TGGTGGCTGT GGTCAACGAC
ACAGTGGGCA CCATGATGAC CTGTGCTTAT GAGGAGCCCA CCTGTGAGGT TGGACTCATT
GTTGGGACCG GCAGCAATGC CTGCTACATG GAGGAGATGA AGAACGTGGA GATGGTGGAG
GGGGACCAGG GGCAGATGTG CATCAACATG GAGTGGGGGG CCTTTGGGGA CAACGGGTGT
CTGGATGATA TCAGGACACA CTACGACAGA CTGGTGGACG AATATTCCCT AAATGCTGGG
AAACAAAGGT ATGAGAAGAT GATCAGTGGT ATGTACCTGG GTGAAATCGT CCGCAACATC
TTAATCGACT TCACCAAGAA GGGATTCCTC TTCCGAGGGC AGATCTCTGA GACGCTGAAG
ACCCGGGGCA TCTTTGAGAC CAAGTTTCTC TCTCAGATCG AGAGTGACCG ATTAGCACTG
CTCCAGGTCC GGGCTATCCT CCAGCAGCTA GGTCTGAATA GCACCTGCGA TGACAGTATC
CTCGTCAAGA CAGTGTGCGG GGTGGTGTCC AGGAGGGCCG CACAGCTGTG TGGCGCAGGC
ATGGCTGCGG TTGTGGATAA GATCCGCGAG AACAGAGGAC TGGACCGTCT GAATGTGACT
GTGGGAGTGG ACGGGACACT CTACAAGCTT CATCCACACT TCTCCAGAAT CATGCACCAG
ACGGTGAAGG AACTGTCACC AAAATGTAAC GTGTCCTTCC TCCTGTCTGA GGATGGCAGC
GGCAAGGGGG CCGCCCTCAT CACGGCCGTG GGCGTGCGGT TACGCACAGA GGCAAGCAGC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269792.1
CDS16..2898
Misc Feature(1)208..1524(+)
Misc Feature(2)385..930(+)
Misc Feature(3)394..840(+)
Misc Feature(4)811..1524(+)
Misc Feature(5)1528..2865(+)
Misc Feature(6)1729..>2220(+)
Misc Feature(7)1738..2184(+)
Misc Feature(8)2158..2865(+)
Translation

Target ORF information:

RefSeq Version XM_005269735
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005269735

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGGGACAG GCCACGACCT GTGGCCCAAG TCCCGCCCAG AGGGAGTGCG CCCTGAGGGA 
GAGCCACCGG AGCTGGTGAC TCGGGGGCGG GTGCTCTGGG GTGTCGCTTT GGGTTCAGCA
ATACAAATCT GCCAGCTGGA GATTAGGCGG GGCACGCCGG GGAGAGGTGA TGCACTGCCG
CTGAAGACCC AGATTGACAA GTATCTCTAT GCCATGCGGC TCTCCGATGA AACTCTCATA
GATATCATGA CTCGCTTCAG GAAGGAGATG AAGAATGGCC TCTCCCGGGA TTTTAATCCA
ACAGCCACAG TCAAGATGTT GCCAACATTC GTAAGGTCCA TTCCTGATGG CTCTGAAAAG
GGAGATTTCA TTGCCCTGGA TCTTGGTGGG TCTTCCTTTC GAATTCTGCG GGTGCAAGTG
AATCATGAGA AAAACCAGAA TGTTCACATG GAGTCCGAGG TTTATGACAC CCCAGAGAAC
ATCGTGCACG GCAGTGGAAG CCAGCTTTTT GATCATGTTG CTGAGTGCCT GGGAGATTTC
ATGGAGAAAA GGAAGATCAA GGACAAGAAG TTACCTGTGG GATTCACGTT TTCTTTTCCT
TGCCAACAAT CCAAAATAGA TGAGGCCATC CTGATCACCT GGACAAAGCG ATTTAAAGCG
AGCGGAGTGG AAGGAGCAGA TGTGGTCAAA CTGCTTAACA AAGCCATCAA AAAGCGAGGG
GACTATGATG CCAACATCGT AGCTGTGGTG AATGACACAG TGGGCACCAT GATGACCTGT
GGCTATGACG ACCAGCACTG TGAAGTCGGC CTGATCATCG GCACTGGCAC CAATGCTTGC
TACATGGAGG AACTGAGGCA CATTGATCTG GTGGAAGGAG ACGAGGGGAG GATGTGTATC
AATACAGAAT GGGGAGCCTT TGGAGACGAT GGATCATTAG AAGACATCCG GACAGAGTTT
GACAGGGAGA TAGACCGGGG ATCCCTCAAC CCTGGAAAAC AGCTGTTTGA GAAGATGGTC
AGTGGCATGT ACTTGGGAGA GCTGGTTCGA CTGATCCTAG TCAAGATGGC CAAGGAGGGC
CTCTTATTTG AAGGGCGGAT CACCCCGGAG CTGCTCACCC GAGGGAAGTT TAACACCAGT
GATGTGTCAG CCATCGAAAA GAATAAGGAA GGCCTCCACA ATGCCAAAGA AATCCTGACC
CGCCTGGGAG TGGAGCCGTC CGATGATGAC TGTGTCTCAG TCCAGCACGT TTGCACCATT
GTCTCATTTC GCTCAGCCAA CTTGGTGGCT GCCACACTGG GCGCCATCTT GAACCGCCTG
CGTGATAACA AGGGCACACC CAGGCTGCGG ACCACGGTTG GTGTCGACGG ATCTCTTTAC
AAGACGCACC CACAGTATTC CCGGCGTTTC CACAAGACTC TAAGGCGCTT GGTGCCAGAC
TCCGATGTGC GCTTCCTCCT CTCGGAGAGT GGCAGCGGCA AGGGGGCTGC CATGGTGACG
GCGGTGGCCT ACCGCTTGGC CGAGCAGCAC CGGCAGATAG AGGAGACCCT GGCTCATTTC
CACCTCACCA AGGACATGCT GCTGGAGGTG AAGAAGAGGA TGCGGGCCGA GATGGAGCTG
GGGCTGAGGA AGCAGACGCA CAACAATGCC GTGGTTAAGA TGCTGCCCTC CTTCGTCCGG
AGAACTCCCG ACGGGACCGA GAATGGTGAC TTCTTGGCCC TGGATCTTGG AGGAACCAAT
TTCCGTGTGC TGCTGGTGAA AATCCGTAGT GGGAAAAAGA GAACGGTGGA AATGCACAAC
AAGATCTACG CCATTCCTAT TGAAATCATG CAGGGCACTG GGGAAGAGCT GTTTGATCAC
ATTGTCTCCT GCATCTCTGA CTTCTTGGAC TACATGGGGA TCAAAGGCCC CAGGATGCCT
CTGGGCTTCA CGTTCTCATT TCCCTGCCAG CAGACGAGTC TGGACGCGGG AATCTTGATC
ACGTGGACAA AGGGTTTTAA GGCAACAGAC TGCGTGGGCC ACGATGTAGT CACCTTACTA
AGGGATGCGA TAAAAAGGAG AGAGGAATTT GACCTGGACG TGGTGGCTGT GGTCAACGAC
ACAGTGGGCA CCATGATGAC CTGTGCTTAT GAGGAGCCCA CCTGTGAGGT TGGACTCATT
GTTGGGACCG GCAGCAATGC CTGCTACATG GAGGAGATGA AGAACGTGGA GATGGTGGAG
GGGGACCAGG GGCAGATGTG CATCAACATG GAGTGGGGGG CCTTTGGGGA CAACGGGTGT
CTGGATGATA TCAGGACACA CTACGACAGA CTGGTGGACG AATATTCCCT AAATGCTGGG
AAACAAAGGT ATGAGAAGAT GATCAGTGGT ATGTACCTGG GTGAAATCGT CCGCAACATC
TTAATCGACT TCACCAAGAA GGGATTCCTC TTCCGAGGGC AGATCTCTGA GACGCTGAAG
ACCCGGGGCA TCTTTGAGAC CAAGTTTCTC TCTCAGATCG AGAGTGACCG ATTAGCACTG
CTCCAGGTCC GGGCTATCCT CCAGCAGCTA GGTCTGAATA GCACCTGCGA TGACAGTATC
CTCGTCAAGA CAGTGTGCGG GGTGGTGTCC AGGAGGGCCG CACAGCTGTG TGGCGCAGGC
ATGGCTGCGG TTGTGGATAA GATCCGCGAG AACAGAGGAC TGGACCGTCT GAATGTGACT
GTGGGAGTGG ACGGGACACT CTACAAGCTT CATCCACACT TCTCCAGAAT CATGCACCAG
ACGGTGAAGG AACTGTCACC AAAATGTAAC GTGTCCTTCC TCCTGTCTGA GGATGGCAGC
GGCAAGGGGG CCGCCCTCAT CACGGCCGTG GGCGTGCGGT TACGCACAGA GGCAAGCAGC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09664
Clone ID Related Accession (Same CDS sequence) NM_033497 , NM_033498 , XM_005269736
Accession Version XM_005269736.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
AGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269793.1
CDS199..2964
Misc Feature(1)244..1590(+)
Misc Feature(2)268..441(+)
Misc Feature(3)451..996(+)
Misc Feature(4)460..906(+)
Misc Feature(5)877..1590(+)
Misc Feature(6)1594..2931(+)
Misc Feature(7)1795..>2286(+)
Misc Feature(8)1804..2250(+)
Misc Feature(9)2224..2931(+)
Translation

Target ORF information:

RefSeq Version XM_005269736
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005269736

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
AGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19393
Clone ID Related Accession (Same CDS sequence) NM_033500 , XM_011539732
Accession Version XM_011539732.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCAAAAA GAGCCCTGCA TGATTTTATT GACAAGTATC TCTATGCCAT GCGGCTCTCC 
GATGAAACTC TCATAGATAT CATGACTCGC TTCAGGAAGG AGATGAAGAA TGGCCTCTCC
CGGGATTTTA ATCCAACAGC CACAGTCAAG ATGTTGCCAA CATTCGTAAG GTCCATTCCT
GATGGCTCTG AAAAGGGAGA TTTCATTGCC CTGGATCTTG GTGGGTCTTC CTTTCGAATT
CTGCGGGTGC AAGTGAATCA TGAGAAAAAC CAGAATGTTC ACATGGAGTC CGAGGTTTAT
GACACCCCAG AGAACATCGT GCACGGCAGT GGAAGCCAGC TTTTTGATCA TGTTGCTGAG
TGCCTGGGAG ATTTCATGGA GAAAAGGAAG ATCAAGGACA AGAAGTTACC TGTGGGATTC
ACGTTTTCTT TTCCTTGCCA ACAATCCAAA ATAGATGAGG CCATCCTGAT CACCTGGACA
AAGCGATTTA AAGCGAGCGG AGTGGAAGGA GCAGATGTGG TCAAACTGCT TAACAAAGCC
ATCAAAAAGC GAGGGGACTA TGATGCCAAC ATCGTAGCTG TGGTGAATGA CACAGTGGGC
ACCATGATGA CCTGTGGCTA TGACGACCAG CACTGTGAAG TCGGCCTGAT CATCGGCACT
GGCACCAATG CTTGCTACAT GGAGGAACTG AGGCACATTG ATCTGGTGGA AGGAGACGAG
GGGAGGATGT GTATCAATAC AGAATGGGGA GCCTTTGGAG ACGATGGATC ATTAGAAGAC
ATCCGGACAG AGTTTGACAG GGAGATAGAC CGGGGATCCC TCAACCCTGG AAAACAGCTG
TTTGAGAAGA TGGTCAGTGG CATGTACTTG GGAGAGCTGG TTCGACTGAT CCTAGTCAAG
ATGGCCAAGG AGGGCCTCTT ATTTGAAGGG CGGATCACCC CGGAGCTGCT CACCCGAGGG
AAGTTTAACA CCAGTGATGT GTCAGCCATC GAAAAGAATA AGGAAGGCCT CCACAATGCC
AAAGAAATCC TGACCCGCCT GGGAGTGGAG CCGTCCGATG ATGACTGTGT CTCAGTCCAG
CACGTTTGCA CCATTGTCTC ATTTCGCTCA GCCAACTTGG TGGCTGCCAC ACTGGGCGCC
ATCTTGAACC GCCTGCGTGA TAACAAGGGC ACACCCAGGC TGCGGACCAC GGTTGGTGTC
GACGGATCTC TTTACAAGAC GCACCCACAG TATTCCCGGC GTTTCCACAA GACTCTAAGG
CGCTTGGTGC CAGACTCCGA TGTGCGCTTC CTCCTCTCGG AGAGTGGCAG CGGCAAGGGG
GCTGCCATGG TGACGGCGGT GGCCTACCGC TTGGCCGAGC AGCACCGGCA GATAGAGGAG
ACCCTGGCTC ATTTCCACCT CACCAAGGAC ATGCTGCTGG AGGTGAAGAA GAGGATGCGG
GCCGAGATGG AGCTGGGGCT GAGGAAGCAG ACGCACAACA ATGCCGTGGT TAAGATGCTG
CCCTCCTTCG TCCGGAGAAC TCCCGACGGG ACCGAGAATG GTGACTTCTT GGCCCTGGAT
CTTGGAGGAA CCAATTTCCG TGTGCTGCTG GTGAAAATCC GTAGTGGGAA AAAGAGAACG
GTGGAAATGC ACAACAAGAT CTACGCCATT CCTATTGAAA TCATGCAGGG CACTGGGGAA
GAGCTGTTTG ATCACATTGT CTCCTGCATC TCTGACTTCT TGGACTACAT GGGGATCAAA
GGCCCCAGGA TGCCTCTGGG CTTCACGTTC TCATTTCCCT GCCAGCAGAC GAGTCTGGAC
GCGGGAATCT TGATCACGTG GACAAAGGGT TTTAAGGCAA CAGACTGCGT GGGCCACGAT
GTAGTCACCT TACTAAGGGA TGCGATAAAA AGGAGAGAGG AATTTGACCT GGACGTGGTG
GCTGTGGTCA ACGACACAGT GGGCACCATG ATGACCTGTG CTTATGAGGA GCCCACCTGT
GAGGTTGGAC TCATTGTTGG GACCGGCAGC AATGCCTGCT ACATGGAGGA GATGAAGAAC
GTGGAGATGG TGGAGGGGGA CCAGGGGCAG ATGTGCATCA ACATGGAGTG GGGGGCCTTT
GGGGACAACG GGTGTCTGGA TGATATCAGG ACACACTACG ACAGACTGGT GGACGAATAT
TCCCTAAATG CTGGGAAACA AAGGTATGAG AAGATGATCA GTGGTATGTA CCTGGGTGAA
ATCGTCCGCA ACATCTTAAT CGACTTCACC AAGAAGGGAT TCCTCTTCCG AGGGCAGATC
TCTGAGACGC TGAAGACCCG GGGCATCTTT GAGACCAAGT TTCTCTCTCA GATCGAGAGT
GACCGATTAG CACTGCTCCA GGTCCGGGCT ATCCTCCAGC AGCTAGGTCT GAATAGCACC
TGCGATGACA GTATCCTCGT CAAGACAGTG TGCGGGGTGG TGTCCAGGAG GGCCGCACAG
CTGTGTGGCG CAGGCATGGC TGCGGTTGTG GATAAGATCC GCGAGAACAG AGGACTGGAC
CGTCTGAATG TGACTGTGGG AGTGGACGGG ACACTCTACA AGCTTCATCC ACACTTCTCC
AGAATCATGC ACCAGACGGT GAAGGAACTG TCACCAAAAT GTAACGTGTC CTTCCTCCTG
TCTGAGGATG GCAGCGGCAA GGGGGCCGCC CTCATCACGG CCGTGGGCGT GCGGTTACGC
ACAGAGGCAA GCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538034.1
CDS407..3124
Misc Feature(1)434..1750(+)
Misc Feature(2)611..1156(+)
Misc Feature(3)620..1066(+)
Misc Feature(4)1037..1750(+)
Misc Feature(5)1754..3091(+)
Misc Feature(6)1955..>2446(+)
Misc Feature(7)1964..2410(+)
Misc Feature(8)2384..3091(+)
Translation

Target ORF information:

RefSeq Version XM_011539732
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539732

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCAAAAA GAGCCCTGCA TGATTTTATT GACAAGTATC TCTATGCCAT GCGGCTCTCC 
GATGAAACTC TCATAGATAT CATGACTCGC TTCAGGAAGG AGATGAAGAA TGGCCTCTCC
CGGGATTTTA ATCCAACAGC CACAGTCAAG ATGTTGCCAA CATTCGTAAG GTCCATTCCT
GATGGCTCTG AAAAGGGAGA TTTCATTGCC CTGGATCTTG GTGGGTCTTC CTTTCGAATT
CTGCGGGTGC AAGTGAATCA TGAGAAAAAC CAGAATGTTC ACATGGAGTC CGAGGTTTAT
GACACCCCAG AGAACATCGT GCACGGCAGT GGAAGCCAGC TTTTTGATCA TGTTGCTGAG
TGCCTGGGAG ATTTCATGGA GAAAAGGAAG ATCAAGGACA AGAAGTTACC TGTGGGATTC
ACGTTTTCTT TTCCTTGCCA ACAATCCAAA ATAGATGAGG CCATCCTGAT CACCTGGACA
AAGCGATTTA AAGCGAGCGG AGTGGAAGGA GCAGATGTGG TCAAACTGCT TAACAAAGCC
ATCAAAAAGC GAGGGGACTA TGATGCCAAC ATCGTAGCTG TGGTGAATGA CACAGTGGGC
ACCATGATGA CCTGTGGCTA TGACGACCAG CACTGTGAAG TCGGCCTGAT CATCGGCACT
GGCACCAATG CTTGCTACAT GGAGGAACTG AGGCACATTG ATCTGGTGGA AGGAGACGAG
GGGAGGATGT GTATCAATAC AGAATGGGGA GCCTTTGGAG ACGATGGATC ATTAGAAGAC
ATCCGGACAG AGTTTGACAG GGAGATAGAC CGGGGATCCC TCAACCCTGG AAAACAGCTG
TTTGAGAAGA TGGTCAGTGG CATGTACTTG GGAGAGCTGG TTCGACTGAT CCTAGTCAAG
ATGGCCAAGG AGGGCCTCTT ATTTGAAGGG CGGATCACCC CGGAGCTGCT CACCCGAGGG
AAGTTTAACA CCAGTGATGT GTCAGCCATC GAAAAGAATA AGGAAGGCCT CCACAATGCC
AAAGAAATCC TGACCCGCCT GGGAGTGGAG CCGTCCGATG ATGACTGTGT CTCAGTCCAG
CACGTTTGCA CCATTGTCTC ATTTCGCTCA GCCAACTTGG TGGCTGCCAC ACTGGGCGCC
ATCTTGAACC GCCTGCGTGA TAACAAGGGC ACACCCAGGC TGCGGACCAC GGTTGGTGTC
GACGGATCTC TTTACAAGAC GCACCCACAG TATTCCCGGC GTTTCCACAA GACTCTAAGG
CGCTTGGTGC CAGACTCCGA TGTGCGCTTC CTCCTCTCGG AGAGTGGCAG CGGCAAGGGG
GCTGCCATGG TGACGGCGGT GGCCTACCGC TTGGCCGAGC AGCACCGGCA GATAGAGGAG
ACCCTGGCTC ATTTCCACCT CACCAAGGAC ATGCTGCTGG AGGTGAAGAA GAGGATGCGG
GCCGAGATGG AGCTGGGGCT GAGGAAGCAG ACGCACAACA ATGCCGTGGT TAAGATGCTG
CCCTCCTTCG TCCGGAGAAC TCCCGACGGG ACCGAGAATG GTGACTTCTT GGCCCTGGAT
CTTGGAGGAA CCAATTTCCG TGTGCTGCTG GTGAAAATCC GTAGTGGGAA AAAGAGAACG
GTGGAAATGC ACAACAAGAT CTACGCCATT CCTATTGAAA TCATGCAGGG CACTGGGGAA
GAGCTGTTTG ATCACATTGT CTCCTGCATC TCTGACTTCT TGGACTACAT GGGGATCAAA
GGCCCCAGGA TGCCTCTGGG CTTCACGTTC TCATTTCCCT GCCAGCAGAC GAGTCTGGAC
GCGGGAATCT TGATCACGTG GACAAAGGGT TTTAAGGCAA CAGACTGCGT GGGCCACGAT
GTAGTCACCT TACTAAGGGA TGCGATAAAA AGGAGAGAGG AATTTGACCT GGACGTGGTG
GCTGTGGTCA ACGACACAGT GGGCACCATG ATGACCTGTG CTTATGAGGA GCCCACCTGT
GAGGTTGGAC TCATTGTTGG GACCGGCAGC AATGCCTGCT ACATGGAGGA GATGAAGAAC
GTGGAGATGG TGGAGGGGGA CCAGGGGCAG ATGTGCATCA ACATGGAGTG GGGGGCCTTT
GGGGACAACG GGTGTCTGGA TGATATCAGG ACACACTACG ACAGACTGGT GGACGAATAT
TCCCTAAATG CTGGGAAACA AAGGTATGAG AAGATGATCA GTGGTATGTA CCTGGGTGAA
ATCGTCCGCA ACATCTTAAT CGACTTCACC AAGAAGGGAT TCCTCTTCCG AGGGCAGATC
TCTGAGACGC TGAAGACCCG GGGCATCTTT GAGACCAAGT TTCTCTCTCA GATCGAGAGT
GACCGATTAG CACTGCTCCA GGTCCGGGCT ATCCTCCAGC AGCTAGGTCT GAATAGCACC
TGCGATGACA GTATCCTCGT CAAGACAGTG TGCGGGGTGG TGTCCAGGAG GGCCGCACAG
CTGTGTGGCG CAGGCATGGC TGCGGTTGTG GATAAGATCC GCGAGAACAG AGGACTGGAC
CGTCTGAATG TGACTGTGGG AGTGGACGGG ACACTCTACA AGCTTCATCC ACACTTCTCC
AGAATCATGC ACCAGACGGT GAAGGAACTG TCACCAAAAT GTAACGTGTC CTTCCTCCTG
TCTGAGGATG GCAGCGGCAA GGGGGCCGCC CTCATCACGG CCGTGGGCGT GCGGTTACGC
ACAGAGGCAA GCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58217
Clone ID Related Accession (Same CDS sequence) XM_011539733
Accession Version XM_011539733.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2712bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCATGTG CTCCTGAGAA GATTGACAAG TATCTCTATG CCATGCGGCT CTCCGATGAA 
ACTCTCATAG ATATCATGAC TCGCTTCAGG AAGGAGATGA AGAATGGCCT CTCCCGGGAT
TTTAATCCAA CAGCCACAGT CAAGATGTTG CCAACATTCG TAAGGTCCAT TCCTGATGGC
TCTGAAAAGG GAGATTTCAT TGCCCTGGAT CTTGGTGGGT CTTCCTTTCG AATTCTGCGG
GTGCAAGTGA ATCATGAGAA AAACCAGAAT GTTCACATGG AGTCCGAGGT TTATGACACC
CCAGAGAACA TCGTGCACGG CAGTGGAAGC CAGCTTTTTG ATCATGTTGC TGAGTGCCTG
GGAGATTTCA TGGAGAAAAG GAAGATCAAG GACAAGAAGT TACCTGTGGG ATTCACGTTT
TCTTTTCCTT GCCAACAATC CAAAATAGAT GAGGCCATCC TGATCACCTG GACAAAGCGA
TTTAAAGCGA GCGGAGTGGA AGGAGCAGAT GTGGTCAAAC TGCTTAACAA AGCCATCAAA
AAGCGAGGGG ACTATGATGC CAACATCGTA GCTGTGGTGA ATGACACAGT GGGCACCATG
ATGACCTGTG GCTATGACGA CCAGCACTGT GAAGTCGGCC TGATCATCGG CACTGGCACC
AATGCTTGCT ACATGGAGGA ACTGAGGCAC ATTGATCTGG TGGAAGGAGA CGAGGGGAGG
ATGTGTATCA ATACAGAATG GGGAGCCTTT GGAGACGATG GATCATTAGA AGACATCCGG
ACAGAGTTTG ACAGGGAGAT AGACCGGGGA TCCCTCAACC CTGGAAAACA GCTGTTTGAG
AAGATGGTCA GTGGCATGTA CTTGGGAGAG CTGGTTCGAC TGATCCTAGT CAAGATGGCC
AAGGAGGGCC TCTTATTTGA AGGGCGGATC ACCCCGGAGC TGCTCACCCG AGGGAAGTTT
AACACCAGTG ATGTGTCAGC CATCGAAAAG AATAAGGAAG GCCTCCACAA TGCCAAAGAA
ATCCTGACCC GCCTGGGAGT GGAGCCGTCC GATGATGACT GTGTCTCAGT CCAGCACGTT
TGCACCATTG TCTCATTTCG CTCAGCCAAC TTGGTGGCTG CCACACTGGG CGCCATCTTG
AACCGCCTGC GTGATAACAA GGGCACACCC AGGCTGCGGA CCACGGTTGG TGTCGACGGA
TCTCTTTACA AGACGCACCC ACAGTATTCC CGGCGTTTCC ACAAGACTCT AAGGCGCTTG
GTGCCAGACT CCGATGTGCG CTTCCTCCTC TCGGAGAGTG GCAGCGGCAA GGGGGCTGCC
ATGGTGACGG CGGTGGCCTA CCGCTTGGCC GAGCAGCACC GGCAGATAGA GGAGACCCTG
GCTCATTTCC ACCTCACCAA GGACATGCTG CTGGAGGTGA AGAAGAGGAT GCGGGCCGAG
ATGGAGCTGG GGCTGAGGAA GCAGACGCAC AACAATGCCG TGGTTAAGAT GCTGCCCTCC
TTCGTCCGGA GAACTCCCGA CGGGACCGAG AATGGTGACT TCTTGGCCCT GGATCTTGGA
GGAACCAATT TCCGTGTGCT GCTGGTGAAA ATCCGTAGTG GGAAAAAGAG AACGGTGGAA
ATGCACAACA AGATCTACGC CATTCCTATT GAAATCATGC AGGGCACTGG GGAAGAGCTG
TTTGATCACA TTGTCTCCTG CATCTCTGAC TTCTTGGACT ACATGGGGAT CAAAGGCCCC
AGGATGCCTC TGGGCTTCAC GTTCTCATTT CCCTGCCAGC AGACGAGTCT GGACGCGGGA
ATCTTGATCA CGTGGACAAA GGGTTTTAAG GCAACAGACT GCGTGGGCCA CGATGTAGTC
ACCTTACTAA GGGATGCGAT AAAAAGGAGA GAGGAATTTG ACCTGGACGT GGTGGCTGTG
GTCAACGACA CAGTGGGCAC CATGATGACC TGTGCTTATG AGGAGCCCAC CTGTGAGGTT
GGACTCATTG TTGGGACCGG CAGCAATGCC TGCTACATGG AGGAGATGAA GAACGTGGAG
ATGGTGGAGG GGGACCAGGG GCAGATGTGC ATCAACATGG AGTGGGGGGC CTTTGGGGAC
AACGGGTGTC TGGATGATAT CAGGACACAC TACGACAGAC TGGTGGACGA ATATTCCCTA
AATGCTGGGA AACAAAGGTA TGAGAAGATG ATCAGTGGTA TGTACCTGGG TGAAATCGTC
CGCAACATCT TAATCGACTT CACCAAGAAG GGATTCCTCT TCCGAGGGCA GATCTCTGAG
ACGCTGAAGA CCCGGGGCAT CTTTGAGACC AAGTTTCTCT CTCAGATCGA GAGTGACCGA
TTAGCACTGC TCCAGGTCCG GGCTATCCTC CAGCAGCTAG GTCTGAATAG CACCTGCGAT
GACAGTATCC TCGTCAAGAC AGTGTGCGGG GTGGTGTCCA GGAGGGCCGC ACAGCTGTGT
GGCGCAGGCA TGGCTGCGGT TGTGGATAAG ATCCGCGAGA ACAGAGGACT GGACCGTCTG
AATGTGACTG TGGGAGTGGA CGGGACACTC TACAAGCTTC ATCCACACTT CTCCAGAATC
ATGCACCAGA CGGTGAAGGA ACTGTCACCA AAATGTAACG TGTCCTTCCT CCTGTCTGAG
GATGGCAGCG GCAAGGGGGC CGCCCTCATC ACGGCCGTGG GCGTGCGGTT ACGCACAGAG
GCAAGCAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538035.1
CDS16..2727
Misc Feature(1)22..204(+)
Misc Feature(2)31..1353(+)
Misc Feature(3)214..759(+)
Misc Feature(4)223..669(+)
Misc Feature(5)640..1353(+)
Misc Feature(6)1357..2694(+)
Misc Feature(7)1558..>2049(+)
Misc Feature(8)1567..2013(+)
Misc Feature(9)1987..2694(+)
Translation

Target ORF information:

RefSeq Version XM_011539733
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539733

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCATGTG CTCCTGAGAA GATTGACAAG TATCTCTATG CCATGCGGCT CTCCGATGAA 
ACTCTCATAG ATATCATGAC TCGCTTCAGG AAGGAGATGA AGAATGGCCT CTCCCGGGAT
TTTAATCCAA CAGCCACAGT CAAGATGTTG CCAACATTCG TAAGGTCCAT TCCTGATGGC
TCTGAAAAGG GAGATTTCAT TGCCCTGGAT CTTGGTGGGT CTTCCTTTCG AATTCTGCGG
GTGCAAGTGA ATCATGAGAA AAACCAGAAT GTTCACATGG AGTCCGAGGT TTATGACACC
CCAGAGAACA TCGTGCACGG CAGTGGAAGC CAGCTTTTTG ATCATGTTGC TGAGTGCCTG
GGAGATTTCA TGGAGAAAAG GAAGATCAAG GACAAGAAGT TACCTGTGGG ATTCACGTTT
TCTTTTCCTT GCCAACAATC CAAAATAGAT GAGGCCATCC TGATCACCTG GACAAAGCGA
TTTAAAGCGA GCGGAGTGGA AGGAGCAGAT GTGGTCAAAC TGCTTAACAA AGCCATCAAA
AAGCGAGGGG ACTATGATGC CAACATCGTA GCTGTGGTGA ATGACACAGT GGGCACCATG
ATGACCTGTG GCTATGACGA CCAGCACTGT GAAGTCGGCC TGATCATCGG CACTGGCACC
AATGCTTGCT ACATGGAGGA ACTGAGGCAC ATTGATCTGG TGGAAGGAGA CGAGGGGAGG
ATGTGTATCA ATACAGAATG GGGAGCCTTT GGAGACGATG GATCATTAGA AGACATCCGG
ACAGAGTTTG ACAGGGAGAT AGACCGGGGA TCCCTCAACC CTGGAAAACA GCTGTTTGAG
AAGATGGTCA GTGGCATGTA CTTGGGAGAG CTGGTTCGAC TGATCCTAGT CAAGATGGCC
AAGGAGGGCC TCTTATTTGA AGGGCGGATC ACCCCGGAGC TGCTCACCCG AGGGAAGTTT
AACACCAGTG ATGTGTCAGC CATCGAAAAG AATAAGGAAG GCCTCCACAA TGCCAAAGAA
ATCCTGACCC GCCTGGGAGT GGAGCCGTCC GATGATGACT GTGTCTCAGT CCAGCACGTT
TGCACCATTG TCTCATTTCG CTCAGCCAAC TTGGTGGCTG CCACACTGGG CGCCATCTTG
AACCGCCTGC GTGATAACAA GGGCACACCC AGGCTGCGGA CCACGGTTGG TGTCGACGGA
TCTCTTTACA AGACGCACCC ACAGTATTCC CGGCGTTTCC ACAAGACTCT AAGGCGCTTG
GTGCCAGACT CCGATGTGCG CTTCCTCCTC TCGGAGAGTG GCAGCGGCAA GGGGGCTGCC
ATGGTGACGG CGGTGGCCTA CCGCTTGGCC GAGCAGCACC GGCAGATAGA GGAGACCCTG
GCTCATTTCC ACCTCACCAA GGACATGCTG CTGGAGGTGA AGAAGAGGAT GCGGGCCGAG
ATGGAGCTGG GGCTGAGGAA GCAGACGCAC AACAATGCCG TGGTTAAGAT GCTGCCCTCC
TTCGTCCGGA GAACTCCCGA CGGGACCGAG AATGGTGACT TCTTGGCCCT GGATCTTGGA
GGAACCAATT TCCGTGTGCT GCTGGTGAAA ATCCGTAGTG GGAAAAAGAG AACGGTGGAA
ATGCACAACA AGATCTACGC CATTCCTATT GAAATCATGC AGGGCACTGG GGAAGAGCTG
TTTGATCACA TTGTCTCCTG CATCTCTGAC TTCTTGGACT ACATGGGGAT CAAAGGCCCC
AGGATGCCTC TGGGCTTCAC GTTCTCATTT CCCTGCCAGC AGACGAGTCT GGACGCGGGA
ATCTTGATCA CGTGGACAAA GGGTTTTAAG GCAACAGACT GCGTGGGCCA CGATGTAGTC
ACCTTACTAA GGGATGCGAT AAAAAGGAGA GAGGAATTTG ACCTGGACGT GGTGGCTGTG
GTCAACGACA CAGTGGGCAC CATGATGACC TGTGCTTATG AGGAGCCCAC CTGTGAGGTT
GGACTCATTG TTGGGACCGG CAGCAATGCC TGCTACATGG AGGAGATGAA GAACGTGGAG
ATGGTGGAGG GGGACCAGGG GCAGATGTGC ATCAACATGG AGTGGGGGGC CTTTGGGGAC
AACGGGTGTC TGGATGATAT CAGGACACAC TACGACAGAC TGGTGGACGA ATATTCCCTA
AATGCTGGGA AACAAAGGTA TGAGAAGATG ATCAGTGGTA TGTACCTGGG TGAAATCGTC
CGCAACATCT TAATCGACTT CACCAAGAAG GGATTCCTCT TCCGAGGGCA GATCTCTGAG
ACGCTGAAGA CCCGGGGCAT CTTTGAGACC AAGTTTCTCT CTCAGATCGA GAGTGACCGA
TTAGCACTGC TCCAGGTCCG GGCTATCCTC CAGCAGCTAG GTCTGAATAG CACCTGCGAT
GACAGTATCC TCGTCAAGAC AGTGTGCGGG GTGGTGTCCA GGAGGGCCGC ACAGCTGTGT
GGCGCAGGCA TGGCTGCGGT TGTGGATAAG ATCCGCGAGA ACAGAGGACT GGACCGTCTG
AATGTGACTG TGGGAGTGGA CGGGACACTC TACAAGCTTC ATCCACACTT CTCCAGAATC
ATGCACCAGA CGGTGAAGGA ACTGTCACCA AAATGTAACG TGTCCTTCCT CCTGTCTGAG
GATGGCAGCG GCAAGGGGGC CGCCCTCATC ACGGCCGTGG GCGTGCGGTT ACGCACAGAG
GCAAGCAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58218
Clone ID Related Accession (Same CDS sequence) XM_011539734
Accession Version XM_011539734.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2709bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGAGCTCC TGTGTATGAT TGACAAGTAT CTCTATGCCA TGCGGCTCTC CGATGAAACT 
CTCATAGATA TCATGACTCG CTTCAGGAAG GAGATGAAGA ATGGCCTCTC CCGGGATTTT
AATCCAACAG CCACAGTCAA GATGTTGCCA ACATTCGTAA GGTCCATTCC TGATGGCTCT
GAAAAGGGAG ATTTCATTGC CCTGGATCTT GGTGGGTCTT CCTTTCGAAT TCTGCGGGTG
CAAGTGAATC ATGAGAAAAA CCAGAATGTT CACATGGAGT CCGAGGTTTA TGACACCCCA
GAGAACATCG TGCACGGCAG TGGAAGCCAG CTTTTTGATC ATGTTGCTGA GTGCCTGGGA
GATTTCATGG AGAAAAGGAA GATCAAGGAC AAGAAGTTAC CTGTGGGATT CACGTTTTCT
TTTCCTTGCC AACAATCCAA AATAGATGAG GCCATCCTGA TCACCTGGAC AAAGCGATTT
AAAGCGAGCG GAGTGGAAGG AGCAGATGTG GTCAAACTGC TTAACAAAGC CATCAAAAAG
CGAGGGGACT ATGATGCCAA CATCGTAGCT GTGGTGAATG ACACAGTGGG CACCATGATG
ACCTGTGGCT ATGACGACCA GCACTGTGAA GTCGGCCTGA TCATCGGCAC TGGCACCAAT
GCTTGCTACA TGGAGGAACT GAGGCACATT GATCTGGTGG AAGGAGACGA GGGGAGGATG
TGTATCAATA CAGAATGGGG AGCCTTTGGA GACGATGGAT CATTAGAAGA CATCCGGACA
GAGTTTGACA GGGAGATAGA CCGGGGATCC CTCAACCCTG GAAAACAGCT GTTTGAGAAG
ATGGTCAGTG GCATGTACTT GGGAGAGCTG GTTCGACTGA TCCTAGTCAA GATGGCCAAG
GAGGGCCTCT TATTTGAAGG GCGGATCACC CCGGAGCTGC TCACCCGAGG GAAGTTTAAC
ACCAGTGATG TGTCAGCCAT CGAAAAGAAT AAGGAAGGCC TCCACAATGC CAAAGAAATC
CTGACCCGCC TGGGAGTGGA GCCGTCCGAT GATGACTGTG TCTCAGTCCA GCACGTTTGC
ACCATTGTCT CATTTCGCTC AGCCAACTTG GTGGCTGCCA CACTGGGCGC CATCTTGAAC
CGCCTGCGTG ATAACAAGGG CACACCCAGG CTGCGGACCA CGGTTGGTGT CGACGGATCT
CTTTACAAGA CGCACCCACA GTATTCCCGG CGTTTCCACA AGACTCTAAG GCGCTTGGTG
CCAGACTCCG ATGTGCGCTT CCTCCTCTCG GAGAGTGGCA GCGGCAAGGG GGCTGCCATG
GTGACGGCGG TGGCCTACCG CTTGGCCGAG CAGCACCGGC AGATAGAGGA GACCCTGGCT
CATTTCCACC TCACCAAGGA CATGCTGCTG GAGGTGAAGA AGAGGATGCG GGCCGAGATG
GAGCTGGGGC TGAGGAAGCA GACGCACAAC AATGCCGTGG TTAAGATGCT GCCCTCCTTC
GTCCGGAGAA CTCCCGACGG GACCGAGAAT GGTGACTTCT TGGCCCTGGA TCTTGGAGGA
ACCAATTTCC GTGTGCTGCT GGTGAAAATC CGTAGTGGGA AAAAGAGAAC GGTGGAAATG
CACAACAAGA TCTACGCCAT TCCTATTGAA ATCATGCAGG GCACTGGGGA AGAGCTGTTT
GATCACATTG TCTCCTGCAT CTCTGACTTC TTGGACTACA TGGGGATCAA AGGCCCCAGG
ATGCCTCTGG GCTTCACGTT CTCATTTCCC TGCCAGCAGA CGAGTCTGGA CGCGGGAATC
TTGATCACGT GGACAAAGGG TTTTAAGGCA ACAGACTGCG TGGGCCACGA TGTAGTCACC
TTACTAAGGG ATGCGATAAA AAGGAGAGAG GAATTTGACC TGGACGTGGT GGCTGTGGTC
AACGACACAG TGGGCACCAT GATGACCTGT GCTTATGAGG AGCCCACCTG TGAGGTTGGA
CTCATTGTTG GGACCGGCAG CAATGCCTGC TACATGGAGG AGATGAAGAA CGTGGAGATG
GTGGAGGGGG ACCAGGGGCA GATGTGCATC AACATGGAGT GGGGGGCCTT TGGGGACAAC
GGGTGTCTGG ATGATATCAG GACACACTAC GACAGACTGG TGGACGAATA TTCCCTAAAT
GCTGGGAAAC AAAGGTATGA GAAGATGATC AGTGGTATGT ACCTGGGTGA AATCGTCCGC
AACATCTTAA TCGACTTCAC CAAGAAGGGA TTCCTCTTCC GAGGGCAGAT CTCTGAGACG
CTGAAGACCC GGGGCATCTT TGAGACCAAG TTTCTCTCTC AGATCGAGAG TGACCGATTA
GCACTGCTCC AGGTCCGGGC TATCCTCCAG CAGCTAGGTC TGAATAGCAC CTGCGATGAC
AGTATCCTCG TCAAGACAGT GTGCGGGGTG GTGTCCAGGA GGGCCGCACA GCTGTGTGGC
GCAGGCATGG CTGCGGTTGT GGATAAGATC CGCGAGAACA GAGGACTGGA CCGTCTGAAT
GTGACTGTGG GAGTGGACGG GACACTCTAC AAGCTTCATC CACACTTCTC CAGAATCATG
CACCAGACGG TGAAGGAACT GTCACCAAAA TGTAACGTGT CCTTCCTCCT GTCTGAGGAT
GGCAGCGGCA AGGGGGCCGC CCTCATCACG GCCGTGGGCG TGCGGTTACG CACAGAGGCA
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538036.1
CDS16..2724
Misc Feature(1)34..1350(+)
Misc Feature(2)211..756(+)
Misc Feature(3)220..666(+)
Misc Feature(4)637..1350(+)
Misc Feature(5)1354..2691(+)
Misc Feature(6)1555..>2046(+)
Misc Feature(7)1564..2010(+)
Misc Feature(8)1984..2691(+)
Translation

Target ORF information:

RefSeq Version XM_011539734
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539734

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGAGCTCC TGTGTATGAT TGACAAGTAT CTCTATGCCA TGCGGCTCTC CGATGAAACT 
CTCATAGATA TCATGACTCG CTTCAGGAAG GAGATGAAGA ATGGCCTCTC CCGGGATTTT
AATCCAACAG CCACAGTCAA GATGTTGCCA ACATTCGTAA GGTCCATTCC TGATGGCTCT
GAAAAGGGAG ATTTCATTGC CCTGGATCTT GGTGGGTCTT CCTTTCGAAT TCTGCGGGTG
CAAGTGAATC ATGAGAAAAA CCAGAATGTT CACATGGAGT CCGAGGTTTA TGACACCCCA
GAGAACATCG TGCACGGCAG TGGAAGCCAG CTTTTTGATC ATGTTGCTGA GTGCCTGGGA
GATTTCATGG AGAAAAGGAA GATCAAGGAC AAGAAGTTAC CTGTGGGATT CACGTTTTCT
TTTCCTTGCC AACAATCCAA AATAGATGAG GCCATCCTGA TCACCTGGAC AAAGCGATTT
AAAGCGAGCG GAGTGGAAGG AGCAGATGTG GTCAAACTGC TTAACAAAGC CATCAAAAAG
CGAGGGGACT ATGATGCCAA CATCGTAGCT GTGGTGAATG ACACAGTGGG CACCATGATG
ACCTGTGGCT ATGACGACCA GCACTGTGAA GTCGGCCTGA TCATCGGCAC TGGCACCAAT
GCTTGCTACA TGGAGGAACT GAGGCACATT GATCTGGTGG AAGGAGACGA GGGGAGGATG
TGTATCAATA CAGAATGGGG AGCCTTTGGA GACGATGGAT CATTAGAAGA CATCCGGACA
GAGTTTGACA GGGAGATAGA CCGGGGATCC CTCAACCCTG GAAAACAGCT GTTTGAGAAG
ATGGTCAGTG GCATGTACTT GGGAGAGCTG GTTCGACTGA TCCTAGTCAA GATGGCCAAG
GAGGGCCTCT TATTTGAAGG GCGGATCACC CCGGAGCTGC TCACCCGAGG GAAGTTTAAC
ACCAGTGATG TGTCAGCCAT CGAAAAGAAT AAGGAAGGCC TCCACAATGC CAAAGAAATC
CTGACCCGCC TGGGAGTGGA GCCGTCCGAT GATGACTGTG TCTCAGTCCA GCACGTTTGC
ACCATTGTCT CATTTCGCTC AGCCAACTTG GTGGCTGCCA CACTGGGCGC CATCTTGAAC
CGCCTGCGTG ATAACAAGGG CACACCCAGG CTGCGGACCA CGGTTGGTGT CGACGGATCT
CTTTACAAGA CGCACCCACA GTATTCCCGG CGTTTCCACA AGACTCTAAG GCGCTTGGTG
CCAGACTCCG ATGTGCGCTT CCTCCTCTCG GAGAGTGGCA GCGGCAAGGG GGCTGCCATG
GTGACGGCGG TGGCCTACCG CTTGGCCGAG CAGCACCGGC AGATAGAGGA GACCCTGGCT
CATTTCCACC TCACCAAGGA CATGCTGCTG GAGGTGAAGA AGAGGATGCG GGCCGAGATG
GAGCTGGGGC TGAGGAAGCA GACGCACAAC AATGCCGTGG TTAAGATGCT GCCCTCCTTC
GTCCGGAGAA CTCCCGACGG GACCGAGAAT GGTGACTTCT TGGCCCTGGA TCTTGGAGGA
ACCAATTTCC GTGTGCTGCT GGTGAAAATC CGTAGTGGGA AAAAGAGAAC GGTGGAAATG
CACAACAAGA TCTACGCCAT TCCTATTGAA ATCATGCAGG GCACTGGGGA AGAGCTGTTT
GATCACATTG TCTCCTGCAT CTCTGACTTC TTGGACTACA TGGGGATCAA AGGCCCCAGG
ATGCCTCTGG GCTTCACGTT CTCATTTCCC TGCCAGCAGA CGAGTCTGGA CGCGGGAATC
TTGATCACGT GGACAAAGGG TTTTAAGGCA ACAGACTGCG TGGGCCACGA TGTAGTCACC
TTACTAAGGG ATGCGATAAA AAGGAGAGAG GAATTTGACC TGGACGTGGT GGCTGTGGTC
AACGACACAG TGGGCACCAT GATGACCTGT GCTTATGAGG AGCCCACCTG TGAGGTTGGA
CTCATTGTTG GGACCGGCAG CAATGCCTGC TACATGGAGG AGATGAAGAA CGTGGAGATG
GTGGAGGGGG ACCAGGGGCA GATGTGCATC AACATGGAGT GGGGGGCCTT TGGGGACAAC
GGGTGTCTGG ATGATATCAG GACACACTAC GACAGACTGG TGGACGAATA TTCCCTAAAT
GCTGGGAAAC AAAGGTATGA GAAGATGATC AGTGGTATGT ACCTGGGTGA AATCGTCCGC
AACATCTTAA TCGACTTCAC CAAGAAGGGA TTCCTCTTCC GAGGGCAGAT CTCTGAGACG
CTGAAGACCC GGGGCATCTT TGAGACCAAG TTTCTCTCTC AGATCGAGAG TGACCGATTA
GCACTGCTCC AGGTCCGGGC TATCCTCCAG CAGCTAGGTC TGAATAGCAC CTGCGATGAC
AGTATCCTCG TCAAGACAGT GTGCGGGGTG GTGTCCAGGA GGGCCGCACA GCTGTGTGGC
GCAGGCATGG CTGCGGTTGT GGATAAGATC CGCGAGAACA GAGGACTGGA CCGTCTGAAT
GTGACTGTGG GAGTGGACGG GACACTCTAC AAGCTTCATC CACACTTCTC CAGAATCATG
CACCAGACGG TGAAGGAACT GTCACCAAAA TGTAACGTGT CCTTCCTCCT GTCTGAGGAT
GGCAGCGGCA AGGGGGCCGC CCTCATCACG GCCGTGGGCG TGCGGTTACG CACAGAGGCA
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu44256
Clone ID Related Accession (Same CDS sequence) XM_005269737
Accession Version XM_005269737.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2670bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCGGCTCT CCGATGAAAC TCTCATAGAT ATCATGACTC GCTTCAGGAA GGAGATGAAG 
AATGGCCTCT CCCGGGATTT TAATCCAACA GCCACAGTCA AGATGTTGCC AACATTCGTA
AGGTCCATTC CTGATGGCTC TGAAAAGGGA GATTTCATTG CCCTGGATCT TGGTGGGTCT
TCCTTTCGAA TTCTGCGGGT GCAAGTGAAT CATGAGAAAA ACCAGAATGT TCACATGGAG
TCCGAGGTTT ATGACACCCC AGAGAACATC GTGCACGGCA GTGGAAGCCA GCTTTTTGAT
CATGTTGCTG AGTGCCTGGG AGATTTCATG GAGAAAAGGA AGATCAAGGA CAAGAAGTTA
CCTGTGGGAT TCACGTTTTC TTTTCCTTGC CAACAATCCA AAATAGATGA GGCCATCCTG
ATCACCTGGA CAAAGCGATT TAAAGCGAGC GGAGTGGAAG GAGCAGATGT GGTCAAACTG
CTTAACAAAG CCATCAAAAA GCGAGGGGAC TATGATGCCA ACATCGTAGC TGTGGTGAAT
GACACAGTGG GCACCATGAT GACCTGTGGC TATGACGACC AGCACTGTGA AGTCGGCCTG
ATCATCGGCA CTGGCACCAA TGCTTGCTAC ATGGAGGAAC TGAGGCACAT TGATCTGGTG
GAAGGAGACG AGGGGAGGAT GTGTATCAAT ACAGAATGGG GAGCCTTTGG AGACGATGGA
TCATTAGAAG ACATCCGGAC AGAGTTTGAC AGGGAGATAG ACCGGGGATC CCTCAACCCT
GGAAAACAGC TGTTTGAGAA GATGGTCAGT GGCATGTACT TGGGAGAGCT GGTTCGACTG
ATCCTAGTCA AGATGGCCAA GGAGGGCCTC TTATTTGAAG GGCGGATCAC CCCGGAGCTG
CTCACCCGAG GGAAGTTTAA CACCAGTGAT GTGTCAGCCA TCGAAAAGAA TAAGGAAGGC
CTCCACAATG CCAAAGAAAT CCTGACCCGC CTGGGAGTGG AGCCGTCCGA TGATGACTGT
GTCTCAGTCC AGCACGTTTG CACCATTGTC TCATTTCGCT CAGCCAACTT GGTGGCTGCC
ACACTGGGCG CCATCTTGAA CCGCCTGCGT GATAACAAGG GCACACCCAG GCTGCGGACC
ACGGTTGGTG TCGACGGATC TCTTTACAAG ACGCACCCAC AGTATTCCCG GCGTTTCCAC
AAGACTCTAA GGCGCTTGGT GCCAGACTCC GATGTGCGCT TCCTCCTCTC GGAGAGTGGC
AGCGGCAAGG GGGCTGCCAT GGTGACGGCG GTGGCCTACC GCTTGGCCGA GCAGCACCGG
CAGATAGAGG AGACCCTGGC TCATTTCCAC CTCACCAAGG ACATGCTGCT GGAGGTGAAG
AAGAGGATGC GGGCCGAGAT GGAGCTGGGG CTGAGGAAGC AGACGCACAA CAATGCCGTG
GTTAAGATGC TGCCCTCCTT CGTCCGGAGA ACTCCCGACG GGACCGAGAA TGGTGACTTC
TTGGCCCTGG ATCTTGGAGG AACCAATTTC CGTGTGCTGC TGGTGAAAAT CCGTAGTGGG
AAAAAGAGAA CGGTGGAAAT GCACAACAAG ATCTACGCCA TTCCTATTGA AATCATGCAG
GGCACTGGGG AAGAGCTGTT TGATCACATT GTCTCCTGCA TCTCTGACTT CTTGGACTAC
ATGGGGATCA AAGGCCCCAG GATGCCTCTG GGCTTCACGT TCTCATTTCC CTGCCAGCAG
ACGAGTCTGG ACGCGGGAAT CTTGATCACG TGGACAAAGG GTTTTAAGGC AACAGACTGC
GTGGGCCACG ATGTAGTCAC CTTACTAAGG GATGCGATAA AAAGGAGAGA GGAATTTGAC
CTGGACGTGG TGGCTGTGGT CAACGACACA GTGGGCACCA TGATGACCTG TGCTTATGAG
GAGCCCACCT GTGAGGTTGG ACTCATTGTT GGGACCGGCA GCAATGCCTG CTACATGGAG
GAGATGAAGA ACGTGGAGAT GGTGGAGGGG GACCAGGGGC AGATGTGCAT CAACATGGAG
TGGGGGGCCT TTGGGGACAA CGGGTGTCTG GATGATATCA GGACACACTA CGACAGACTG
GTGGACGAAT ATTCCCTAAA TGCTGGGAAA CAAAGGTATG AGAAGATGAT CAGTGGTATG
TACCTGGGTG AAATCGTCCG CAACATCTTA ATCGACTTCA CCAAGAAGGG ATTCCTCTTC
CGAGGGCAGA TCTCTGAGAC GCTGAAGACC CGGGGCATCT TTGAGACCAA GTTTCTCTCT
CAGATCGAGA GTGACCGATT AGCACTGCTC CAGGTCCGGG CTATCCTCCA GCAGCTAGGT
CTGAATAGCA CCTGCGATGA CAGTATCCTC GTCAAGACAG TGTGCGGGGT GGTGTCCAGG
AGGGCCGCAC AGCTGTGTGG CGCAGGCATG GCTGCGGTTG TGGATAAGAT CCGCGAGAAC
AGAGGACTGG ACCGTCTGAA TGTGACTGTG GGAGTGGACG GGACACTCTA CAAGCTTCAT
CCACACTTCT CCAGAATCAT GCACCAGACG GTGAAGGAAC TGTCACCAAA ATGTAACGTG
TCCTTCCTCC TGTCTGAGGA TGGCAGCGGC AAGGGGGCCG CCCTCATCAC GGCCGTGGGC
GTGCGGTTAC GCACAGAGGC AAGCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269794.1
CDS457..3126
Misc Feature(1)457..1752(+)
Misc Feature(2)613..1158(+)
Misc Feature(3)622..1068(+)
Misc Feature(4)1039..1752(+)
Misc Feature(5)1756..3093(+)
Misc Feature(6)1957..>2448(+)
Misc Feature(7)1966..2412(+)
Misc Feature(8)2386..3093(+)
Translation

Target ORF information:

RefSeq Version XM_005269737
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hexokinase 1 (HK1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005269737

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCGGCTCT CCGATGAAAC TCTCATAGAT ATCATGACTC GCTTCAGGAA GGAGATGAAG 
AATGGCCTCT CCCGGGATTT TAATCCAACA GCCACAGTCA AGATGTTGCC AACATTCGTA
AGGTCCATTC CTGATGGCTC TGAAAAGGGA GATTTCATTG CCCTGGATCT TGGTGGGTCT
TCCTTTCGAA TTCTGCGGGT GCAAGTGAAT CATGAGAAAA ACCAGAATGT TCACATGGAG
TCCGAGGTTT ATGACACCCC AGAGAACATC GTGCACGGCA GTGGAAGCCA GCTTTTTGAT
CATGTTGCTG AGTGCCTGGG AGATTTCATG GAGAAAAGGA AGATCAAGGA CAAGAAGTTA
CCTGTGGGAT TCACGTTTTC TTTTCCTTGC CAACAATCCA AAATAGATGA GGCCATCCTG
ATCACCTGGA CAAAGCGATT TAAAGCGAGC GGAGTGGAAG GAGCAGATGT GGTCAAACTG
CTTAACAAAG CCATCAAAAA GCGAGGGGAC TATGATGCCA ACATCGTAGC TGTGGTGAAT
GACACAGTGG GCACCATGAT GACCTGTGGC TATGACGACC AGCACTGTGA AGTCGGCCTG
ATCATCGGCA CTGGCACCAA TGCTTGCTAC ATGGAGGAAC TGAGGCACAT TGATCTGGTG
GAAGGAGACG AGGGGAGGAT GTGTATCAAT ACAGAATGGG GAGCCTTTGG AGACGATGGA
TCATTAGAAG ACATCCGGAC AGAGTTTGAC AGGGAGATAG ACCGGGGATC CCTCAACCCT
GGAAAACAGC TGTTTGAGAA GATGGTCAGT GGCATGTACT TGGGAGAGCT GGTTCGACTG
ATCCTAGTCA AGATGGCCAA GGAGGGCCTC TTATTTGAAG GGCGGATCAC CCCGGAGCTG
CTCACCCGAG GGAAGTTTAA CACCAGTGAT GTGTCAGCCA TCGAAAAGAA TAAGGAAGGC
CTCCACAATG CCAAAGAAAT CCTGACCCGC CTGGGAGTGG AGCCGTCCGA TGATGACTGT
GTCTCAGTCC AGCACGTTTG CACCATTGTC TCATTTCGCT CAGCCAACTT GGTGGCTGCC
ACACTGGGCG CCATCTTGAA CCGCCTGCGT GATAACAAGG GCACACCCAG GCTGCGGACC
ACGGTTGGTG TCGACGGATC TCTTTACAAG ACGCACCCAC AGTATTCCCG GCGTTTCCAC
AAGACTCTAA GGCGCTTGGT GCCAGACTCC GATGTGCGCT TCCTCCTCTC GGAGAGTGGC
AGCGGCAAGG GGGCTGCCAT GGTGACGGCG GTGGCCTACC GCTTGGCCGA GCAGCACCGG
CAGATAGAGG AGACCCTGGC TCATTTCCAC CTCACCAAGG ACATGCTGCT GGAGGTGAAG
AAGAGGATGC GGGCCGAGAT GGAGCTGGGG CTGAGGAAGC AGACGCACAA CAATGCCGTG
GTTAAGATGC TGCCCTCCTT CGTCCGGAGA ACTCCCGACG GGACCGAGAA TGGTGACTTC
TTGGCCCTGG ATCTTGGAGG AACCAATTTC CGTGTGCTGC TGGTGAAAAT CCGTAGTGGG
AAAAAGAGAA CGGTGGAAAT GCACAACAAG ATCTACGCCA TTCCTATTGA AATCATGCAG
GGCACTGGGG AAGAGCTGTT TGATCACATT GTCTCCTGCA TCTCTGACTT CTTGGACTAC
ATGGGGATCA AAGGCCCCAG GATGCCTCTG GGCTTCACGT TCTCATTTCC CTGCCAGCAG
ACGAGTCTGG ACGCGGGAAT CTTGATCACG TGGACAAAGG GTTTTAAGGC AACAGACTGC
GTGGGCCACG ATGTAGTCAC CTTACTAAGG GATGCGATAA AAAGGAGAGA GGAATTTGAC
CTGGACGTGG TGGCTGTGGT CAACGACACA GTGGGCACCA TGATGACCTG TGCTTATGAG
GAGCCCACCT GTGAGGTTGG ACTCATTGTT GGGACCGGCA GCAATGCCTG CTACATGGAG
GAGATGAAGA ACGTGGAGAT GGTGGAGGGG GACCAGGGGC AGATGTGCAT CAACATGGAG
TGGGGGGCCT TTGGGGACAA CGGGTGTCTG GATGATATCA GGACACACTA CGACAGACTG
GTGGACGAAT ATTCCCTAAA TGCTGGGAAA CAAAGGTATG AGAAGATGAT CAGTGGTATG
TACCTGGGTG AAATCGTCCG CAACATCTTA ATCGACTTCA CCAAGAAGGG ATTCCTCTTC
CGAGGGCAGA TCTCTGAGAC GCTGAAGACC CGGGGCATCT TTGAGACCAA GTTTCTCTCT
CAGATCGAGA GTGACCGATT AGCACTGCTC CAGGTCCGGG CTATCCTCCA GCAGCTAGGT
CTGAATAGCA CCTGCGATGA CAGTATCCTC GTCAAGACAG TGTGCGGGGT GGTGTCCAGG
AGGGCCGCAC AGCTGTGTGG CGCAGGCATG GCTGCGGTTG TGGATAAGAT CCGCGAGAAC
AGAGGACTGG ACCGTCTGAA TGTGACTGTG GGAGTGGACG GGACACTCTA CAAGCTTCAT
CCACACTTCT CCAGAATCAT GCACCAGACG GTGAAGGAAC TGTCACCAAA ATGTAACGTG
TCCTTCCTCC TGTCTGAGGA TGGCAGCGGC AAGGGGGCCG CCCTCATCAC GGCCGTGGGC
GTGCGGTTAC GCACAGAGGC AAGCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09550
Clone ID Related Accession (Same CDS sequence) NM_033496
Accession Version NM_033496.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform HKI-R
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX406321.2, BC008730.2 and AK128226.1. On May 16, 2008 this sequence version replaced gi:15991826. Transcript Variant: Variant 2 (HKI-R) encodes the erythroid-specific isoform. Isoform HKI-R lacks the porin binding domain (PBD), which mediates association with the mitochondrial membrane, and thus localizes to the cytoplasm. Variant 2 includes an erythroid-specific exon in the 5' end which encodes a distinct N-terminus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACTGTG AGCACAGCCT GAGTTTGCCC TGTCGAGGTG CTGAGGCCTG GGAGATTGGG 
ATTGACAAGT ATCTCTATGC CATGCGGCTC TCCGATGAAA CTCTCATAGA TATCATGACT
CGCTTCAGGA AGGAGATGAA GAATGGCCTC TCCCGGGATT TTAATCCAAC AGCCACAGTC
AAGATGTTGC CAACATTCGT AAGGTCCATT CCTGATGGCT CTGAAAAGGG AGATTTCATT
GCCCTGGATC TTGGTGGGTC TTCCTTTCGA ATTCTGCGGG TGCAAGTGAA TCATGAGAAA
AACCAGAATG TTCACATGGA GTCCGAGGTT TATGACACCC CAGAGAACAT CGTGCACGGC
AGTGGAAGCC AGCTTTTTGA TCATGTTGCT GAGTGCCTGG GAGATTTCAT GGAGAAAAGG
AAGATCAAGG ACAAGAAGTT ACCTGTGGGA TTCACGTTTT CTTTTCCTTG CCAACAATCC
AAAATAGATG AGGCCATCCT GATCACCTGG ACAAAGCGAT TTAAAGCGAG CGGAGTGGAA
GGAGCAGATG TGGTCAAACT GCTTAACAAA GCCATCAAAA AGCGAGGGGA CTATGATGCC
AACATCGTAG CTGTGGTGAA TGACACAGTG GGCACCATGA TGACCTGTGG CTATGACGAC
CAGCACTGTG AAGTCGGCCT GATCATCGGC ACTGGCACCA ATGCTTGCTA CATGGAGGAA
CTGAGGCACA TTGATCTGGT GGAAGGAGAC GAGGGGAGGA TGTGTATCAA TACAGAATGG
GGAGCCTTTG GAGACGATGG ATCATTAGAA GACATCCGGA CAGAGTTTGA CAGGGAGATA
GACCGGGGAT CCCTCAACCC TGGAAAACAG CTGTTTGAGA AGATGGTCAG TGGCATGTAC
TTGGGAGAGC TGGTTCGACT GATCCTAGTC AAGATGGCCA AGGAGGGCCT CTTATTTGAA
GGGCGGATCA CCCCGGAGCT GCTCACCCGA GGGAAGTTTA ACACCAGTGA TGTGTCAGCC
ATCGAAAAGA ATAAGGAAGG CCTCCACAAT GCCAAAGAAA TCCTGACCCG CCTGGGAGTG
GAGCCGTCCG ATGATGACTG TGTCTCAGTC CAGCACGTTT GCACCATTGT CTCATTTCGC
TCAGCCAACT TGGTGGCTGC CACACTGGGC GCCATCTTGA ACCGCCTGCG TGATAACAAG
GGCACACCCA GGCTGCGGAC CACGGTTGGT GTCGACGGAT CTCTTTACAA GACGCACCCA
CAGTATTCCC GGCGTTTCCA CAAGACTCTA AGGCGCTTGG TGCCAGACTC CGATGTGCGC
TTCCTCCTCT CGGAGAGTGG CAGCGGCAAG GGGGCTGCCA TGGTGACGGC GGTGGCCTAC
CGCTTGGCCG AGCAGCACCG GCAGATAGAG GAGACCCTGG CTCATTTCCA CCTCACCAAG
GACATGCTGC TGGAGGTGAA GAAGAGGATG CGGGCCGAGA TGGAGCTGGG GCTGAGGAAG
CAGACGCACA ACAATGCCGT GGTTAAGATG CTGCCCTCCT TCGTCCGGAG AACTCCCGAC
GGGACCGAGA ATGGTGACTT CTTGGCCCTG GATCTTGGAG GAACCAATTT CCGTGTGCTG
CTGGTGAAAA TCCGTAGTGG GAAAAAGAGA ACGGTGGAAA TGCACAACAA GATCTACGCC
ATTCCTATTG AAATCATGCA GGGCACTGGG GAAGAGCTGT TTGATCACAT TGTCTCCTGC
ATCTCTGACT TCTTGGACTA CATGGGGATC AAAGGCCCCA GGATGCCTCT GGGCTTCACG
TTCTCATTTC CCTGCCAGCA GACGAGTCTG GACGCGGGAA TCTTGATCAC GTGGACAAAG
GGTTTTAAGG CAACAGACTG CGTGGGCCAC GATGTAGTCA CCTTACTAAG GGATGCGATA
AAAAGGAGAG AGGAATTTGA CCTGGACGTG GTGGCTGTGG TCAACGACAC AGTGGGCACC
ATGATGACCT GTGCTTATGA GGAGCCCACC TGTGAGGTTG GACTCATTGT TGGGACCGGC
AGCAATGCCT GCTACATGGA GGAGATGAAG AACGTGGAGA TGGTGGAGGG GGACCAGGGG
CAGATGTGCA TCAACATGGA GTGGGGGGCC TTTGGGGACA ACGGGTGTCT GGATGATATC
AGGACACACT ACGACAGACT GGTGGACGAA TATTCCCTAA ATGCTGGGAA ACAAAGGTAT
GAGAAGATGA TCAGTGGTAT GTACCTGGGT GAAATCGTCC GCAACATCTT AATCGACTTC
ACCAAGAAGG GATTCCTCTT CCGAGGGCAG ATCTCTGAGA CGCTGAAGAC CCGGGGCATC
TTTGAGACCA AGTTTCTCTC TCAGATCGAG AGTGACCGAT TAGCACTGCT CCAGGTCCGG
GCTATCCTCC AGCAGCTAGG TCTGAATAGC ACCTGCGATG ACAGTATCCT CGTCAAGACA
GTGTGCGGGG TGGTGTCCAG GAGGGCCGCA CAGCTGTGTG GCGCAGGCAT GGCTGCGGTT
GTGGATAAGA TCCGCGAGAA CAGAGGACTG GACCGTCTGA ATGTGACTGT GGGAGTGGAC
GGGACACTCT ACAAGCTTCA TCCACACTTC TCCAGAATCA TGCACCAGAC GGTGAAGGAA
CTGTCACCAA AATGTAACGT GTCCTTCCTC CTGTCTGAGG ATGGCAGCGG CAAGGGGGCC
GCCCTCATCA CGGCCGTGGG CGTGCGGTTA CGCACAGAGG CAAGCAGCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_277031.1
CDS102..2852
Misc Feature(1)75..77(+)
Misc Feature(2)162..1478(+)
Misc Feature(3)339..884(+)
Misc Feature(4)348..794(+)
Misc Feature(5)765..1478(+)
Misc Feature(6)1482..2819(+)
Misc Feature(7)1683..>2174(+)
Misc Feature(8)1692..2138(+)
Misc Feature(9)2112..2819(+)
Exon (1)1..161
Gene:HK1
Gene Synonym:
Exon (2)162..324
Gene:HK1
Gene Synonym:
Exon (3)325..473
Gene:HK1
Gene Synonym:
Exon (4)474..593
Gene:HK1
Gene Synonym:
Exon (5)594..689
Gene:HK1
Gene Synonym:
Exon (6)690..789
Gene:HK1
Gene Synonym:
Exon (7)790..973
Gene:HK1
Gene Synonym:
Exon (8)974..1129
Gene:HK1
Gene Synonym:
Exon (9)1130..1363
Gene:HK1
Gene Synonym:
Exon (10)1364..1668
Gene:HK1
Gene Synonym:
Exon (11)1669..1817
Gene:HK1
Gene Synonym:
Exon (12)1818..1937
Gene:HK1
Gene Synonym:
Exon (13)1938..2033
Gene:HK1
Gene Synonym:
Exon (14)2034..2133
Gene:HK1
Gene Synonym:
Exon (15)2134..2317
Gene:HK1
Gene Synonym:
Exon (16)2318..2473
Gene:HK1
Gene Synonym:
Exon (17)2474..2707
Gene:HK1
Gene Synonym:
Exon (18)2708..3599
Gene:HK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_033496
Organism Homo sapiens (human)
Definition Homo sapiens hexokinase 1 (HK1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033496

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACTGTG AGCACAGCCT GAGTTTGCCC TGTCGAGGTG CTGAGGCCTG GGAGATTGGG 
ATTGACAAGT ATCTCTATGC CATGCGGCTC TCCGATGAAA CTCTCATAGA TATCATGACT
CGCTTCAGGA AGGAGATGAA GAATGGCCTC TCCCGGGATT TTAATCCAAC AGCCACAGTC
AAGATGTTGC CAACATTCGT AAGGTCCATT CCTGATGGCT CTGAAAAGGG AGATTTCATT
GCCCTGGATC TTGGTGGGTC TTCCTTTCGA ATTCTGCGGG TGCAAGTGAA TCATGAGAAA
AACCAGAATG TTCACATGGA GTCCGAGGTT TATGACACCC CAGAGAACAT CGTGCACGGC
AGTGGAAGCC AGCTTTTTGA TCATGTTGCT GAGTGCCTGG GAGATTTCAT GGAGAAAAGG
AAGATCAAGG ACAAGAAGTT ACCTGTGGGA TTCACGTTTT CTTTTCCTTG CCAACAATCC
AAAATAGATG AGGCCATCCT GATCACCTGG ACAAAGCGAT TTAAAGCGAG CGGAGTGGAA
GGAGCAGATG TGGTCAAACT GCTTAACAAA GCCATCAAAA AGCGAGGGGA CTATGATGCC
AACATCGTAG CTGTGGTGAA TGACACAGTG GGCACCATGA TGACCTGTGG CTATGACGAC
CAGCACTGTG AAGTCGGCCT GATCATCGGC ACTGGCACCA ATGCTTGCTA CATGGAGGAA
CTGAGGCACA TTGATCTGGT GGAAGGAGAC GAGGGGAGGA TGTGTATCAA TACAGAATGG
GGAGCCTTTG GAGACGATGG ATCATTAGAA GACATCCGGA CAGAGTTTGA CAGGGAGATA
GACCGGGGAT CCCTCAACCC TGGAAAACAG CTGTTTGAGA AGATGGTCAG TGGCATGTAC
TTGGGAGAGC TGGTTCGACT GATCCTAGTC AAGATGGCCA AGGAGGGCCT CTTATTTGAA
GGGCGGATCA CCCCGGAGCT GCTCACCCGA GGGAAGTTTA ACACCAGTGA TGTGTCAGCC
ATCGAAAAGA ATAAGGAAGG CCTCCACAAT GCCAAAGAAA TCCTGACCCG CCTGGGAGTG
GAGCCGTCCG ATGATGACTG TGTCTCAGTC CAGCACGTTT GCACCATTGT CTCATTTCGC
TCAGCCAACT TGGTGGCTGC CACACTGGGC GCCATCTTGA ACCGCCTGCG TGATAACAAG
GGCACACCCA GGCTGCGGAC CACGGTTGGT GTCGACGGAT CTCTTTACAA GACGCACCCA
CAGTATTCCC GGCGTTTCCA CAAGACTCTA AGGCGCTTGG TGCCAGACTC CGATGTGCGC
TTCCTCCTCT CGGAGAGTGG CAGCGGCAAG GGGGCTGCCA TGGTGACGGC GGTGGCCTAC
CGCTTGGCCG AGCAGCACCG GCAGATAGAG GAGACCCTGG CTCATTTCCA CCTCACCAAG
GACATGCTGC TGGAGGTGAA GAAGAGGATG CGGGCCGAGA TGGAGCTGGG GCTGAGGAAG
CAGACGCACA ACAATGCCGT GGTTAAGATG CTGCCCTCCT TCGTCCGGAG AACTCCCGAC
GGGACCGAGA ATGGTGACTT CTTGGCCCTG GATCTTGGAG GAACCAATTT CCGTGTGCTG
CTGGTGAAAA TCCGTAGTGG GAAAAAGAGA ACGGTGGAAA TGCACAACAA GATCTACGCC
ATTCCTATTG AAATCATGCA GGGCACTGGG GAAGAGCTGT TTGATCACAT TGTCTCCTGC
ATCTCTGACT TCTTGGACTA CATGGGGATC AAAGGCCCCA GGATGCCTCT GGGCTTCACG
TTCTCATTTC CCTGCCAGCA GACGAGTCTG GACGCGGGAA TCTTGATCAC GTGGACAAAG
GGTTTTAAGG CAACAGACTG CGTGGGCCAC GATGTAGTCA CCTTACTAAG GGATGCGATA
AAAAGGAGAG AGGAATTTGA CCTGGACGTG GTGGCTGTGG TCAACGACAC AGTGGGCACC
ATGATGACCT GTGCTTATGA GGAGCCCACC TGTGAGGTTG GACTCATTGT TGGGACCGGC
AGCAATGCCT GCTACATGGA GGAGATGAAG AACGTGGAGA TGGTGGAGGG GGACCAGGGG
CAGATGTGCA TCAACATGGA GTGGGGGGCC TTTGGGGACA ACGGGTGTCT GGATGATATC
AGGACACACT ACGACAGACT GGTGGACGAA TATTCCCTAA ATGCTGGGAA ACAAAGGTAT
GAGAAGATGA TCAGTGGTAT GTACCTGGGT GAAATCGTCC GCAACATCTT AATCGACTTC
ACCAAGAAGG GATTCCTCTT CCGAGGGCAG ATCTCTGAGA CGCTGAAGAC CCGGGGCATC
TTTGAGACCA AGTTTCTCTC TCAGATCGAG AGTGACCGAT TAGCACTGCT CCAGGTCCGG
GCTATCCTCC AGCAGCTAGG TCTGAATAGC ACCTGCGATG ACAGTATCCT CGTCAAGACA
GTGTGCGGGG TGGTGTCCAG GAGGGCCGCA CAGCTGTGTG GCGCAGGCAT GGCTGCGGTT
GTGGATAAGA TCCGCGAGAA CAGAGGACTG GACCGTCTGA ATGTGACTGT GGGAGTGGAC
GGGACACTCT ACAAGCTTCA TCCACACTTC TCCAGAATCA TGCACCAGAC GGTGAAGGAA
CTGTCACCAA AATGTAACGT GTCCTTCCTC CTGTCTGAGG ATGGCAGCGG CAAGGGGGCC
GCCCTCATCA CGGCCGTGGG CGTGCGGTTA CGCACAGAGG CAAGCAGCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19393
Clone ID Related Accession (Same CDS sequence) NM_033500 , XM_011539732
Accession Version NM_033500.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform HKI-td
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U38227.1, AF073786.2, AK128226.1 and BC008730.2. This sequence is a reference standard in the RefSeqGene project. On May 16, 2008 this sequence version replaced gi:15991832. Transcript Variant: Variant 5 (HKI-td) has five testis-specific exons in the 5' end, one of which includes an additional 54 nt fragment unique to variants 4 and 5. Isoform HKI-td has a unique N-terminus and lacks the porin binding domain (PBD) required for association with the mitochondrial membrane. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCAAAAA GAGCCCTGCA TGATTTTATT GACAAGTATC TCTATGCCAT GCGGCTCTCC 
GATGAAACTC TCATAGATAT CATGACTCGC TTCAGGAAGG AGATGAAGAA TGGCCTCTCC
CGGGATTTTA ATCCAACAGC CACAGTCAAG ATGTTGCCAA CATTCGTAAG GTCCATTCCT
GATGGCTCTG AAAAGGGAGA TTTCATTGCC CTGGATCTTG GTGGGTCTTC CTTTCGAATT
CTGCGGGTGC AAGTGAATCA TGAGAAAAAC CAGAATGTTC ACATGGAGTC CGAGGTTTAT
GACACCCCAG AGAACATCGT GCACGGCAGT GGAAGCCAGC TTTTTGATCA TGTTGCTGAG
TGCCTGGGAG ATTTCATGGA GAAAAGGAAG ATCAAGGACA AGAAGTTACC TGTGGGATTC
ACGTTTTCTT TTCCTTGCCA ACAATCCAAA ATAGATGAGG CCATCCTGAT CACCTGGACA
AAGCGATTTA AAGCGAGCGG AGTGGAAGGA GCAGATGTGG TCAAACTGCT TAACAAAGCC
ATCAAAAAGC GAGGGGACTA TGATGCCAAC ATCGTAGCTG TGGTGAATGA CACAGTGGGC
ACCATGATGA CCTGTGGCTA TGACGACCAG CACTGTGAAG TCGGCCTGAT CATCGGCACT
GGCACCAATG CTTGCTACAT GGAGGAACTG AGGCACATTG ATCTGGTGGA AGGAGACGAG
GGGAGGATGT GTATCAATAC AGAATGGGGA GCCTTTGGAG ACGATGGATC ATTAGAAGAC
ATCCGGACAG AGTTTGACAG GGAGATAGAC CGGGGATCCC TCAACCCTGG AAAACAGCTG
TTTGAGAAGA TGGTCAGTGG CATGTACTTG GGAGAGCTGG TTCGACTGAT CCTAGTCAAG
ATGGCCAAGG AGGGCCTCTT ATTTGAAGGG CGGATCACCC CGGAGCTGCT CACCCGAGGG
AAGTTTAACA CCAGTGATGT GTCAGCCATC GAAAAGAATA AGGAAGGCCT CCACAATGCC
AAAGAAATCC TGACCCGCCT GGGAGTGGAG CCGTCCGATG ATGACTGTGT CTCAGTCCAG
CACGTTTGCA CCATTGTCTC ATTTCGCTCA GCCAACTTGG TGGCTGCCAC ACTGGGCGCC
ATCTTGAACC GCCTGCGTGA TAACAAGGGC ACACCCAGGC TGCGGACCAC GGTTGGTGTC
GACGGATCTC TTTACAAGAC GCACCCACAG TATTCCCGGC GTTTCCACAA GACTCTAAGG
CGCTTGGTGC CAGACTCCGA TGTGCGCTTC CTCCTCTCGG AGAGTGGCAG CGGCAAGGGG
GCTGCCATGG TGACGGCGGT GGCCTACCGC TTGGCCGAGC AGCACCGGCA GATAGAGGAG
ACCCTGGCTC ATTTCCACCT CACCAAGGAC ATGCTGCTGG AGGTGAAGAA GAGGATGCGG
GCCGAGATGG AGCTGGGGCT GAGGAAGCAG ACGCACAACA ATGCCGTGGT TAAGATGCTG
CCCTCCTTCG TCCGGAGAAC TCCCGACGGG ACCGAGAATG GTGACTTCTT GGCCCTGGAT
CTTGGAGGAA CCAATTTCCG TGTGCTGCTG GTGAAAATCC GTAGTGGGAA AAAGAGAACG
GTGGAAATGC ACAACAAGAT CTACGCCATT CCTATTGAAA TCATGCAGGG CACTGGGGAA
GAGCTGTTTG ATCACATTGT CTCCTGCATC TCTGACTTCT TGGACTACAT GGGGATCAAA
GGCCCCAGGA TGCCTCTGGG CTTCACGTTC TCATTTCCCT GCCAGCAGAC GAGTCTGGAC
GCGGGAATCT TGATCACGTG GACAAAGGGT TTTAAGGCAA CAGACTGCGT GGGCCACGAT
GTAGTCACCT TACTAAGGGA TGCGATAAAA AGGAGAGAGG AATTTGACCT GGACGTGGTG
GCTGTGGTCA ACGACACAGT GGGCACCATG ATGACCTGTG CTTATGAGGA GCCCACCTGT
GAGGTTGGAC TCATTGTTGG GACCGGCAGC AATGCCTGCT ACATGGAGGA GATGAAGAAC
GTGGAGATGG TGGAGGGGGA CCAGGGGCAG ATGTGCATCA ACATGGAGTG GGGGGCCTTT
GGGGACAACG GGTGTCTGGA TGATATCAGG ACACACTACG ACAGACTGGT GGACGAATAT
TCCCTAAATG CTGGGAAACA AAGGTATGAG AAGATGATCA GTGGTATGTA CCTGGGTGAA
ATCGTCCGCA ACATCTTAAT CGACTTCACC AAGAAGGGAT TCCTCTTCCG AGGGCAGATC
TCTGAGACGC TGAAGACCCG GGGCATCTTT GAGACCAAGT TTCTCTCTCA GATCGAGAGT
GACCGATTAG CACTGCTCCA GGTCCGGGCT ATCCTCCAGC AGCTAGGTCT GAATAGCACC
TGCGATGACA GTATCCTCGT CAAGACAGTG TGCGGGGTGG TGTCCAGGAG GGCCGCACAG
CTGTGTGGCG CAGGCATGGC TGCGGTTGTG GATAAGATCC GCGAGAACAG AGGACTGGAC
CGTCTGAATG TGACTGTGGG AGTGGACGGG ACACTCTACA AGCTTCATCC ACACTTCTCC
AGAATCATGC ACCAGACGGT GAAGGAACTG TCACCAAAAT GTAACGTGTC CTTCCTCCTG
TCTGAGGATG GCAGCGGCAA GGGGGCCGCC CTCATCACGG CCGTGGGCGT GCGGTTACGC
ACAGAGGCAA GCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_277035.2
CDS500..3217
Misc Feature(1)470..472(+)
Misc Feature(2)527..1843(+)
Misc Feature(3)704..1249(+)
Misc Feature(4)713..1159(+)
Misc Feature(5)1130..1843(+)
Misc Feature(6)1847..3184(+)
Misc Feature(7)2048..>2539(+)
Misc Feature(8)2057..2503(+)
Misc Feature(9)2477..3184(+)
Exon (1)1..109
Gene:HK1
Gene Synonym:
Exon (2)110..285
Gene:HK1
Gene Synonym:
Exon (3)286..385
Gene:HK1
Gene Synonym:
Exon (4)386..433
Gene:HK1
Gene Synonym:
Exon (5)434..526
Gene:HK1
Gene Synonym:
Exon (6)527..689
Gene:HK1
Gene Synonym:
Exon (7)690..838
Gene:HK1
Gene Synonym:
Exon (8)839..958
Gene:HK1
Gene Synonym:
Exon (9)959..1054
Gene:HK1
Gene Synonym:
Exon (10)1055..1154
Gene:HK1
Gene Synonym:
Exon (11)1155..1338
Gene:HK1
Gene Synonym:
Exon (12)1339..1494
Gene:HK1
Gene Synonym:
Exon (13)1495..1728
Gene:HK1
Gene Synonym:
Exon (14)1729..2033
Gene:HK1
Gene Synonym:
Exon (15)2034..2182
Gene:HK1
Gene Synonym:
Exon (16)2183..2302
Gene:HK1
Gene Synonym:
Exon (17)2303..2398
Gene:HK1
Gene Synonym:
Exon (18)2399..2498
Gene:HK1
Gene Synonym:
Exon (19)2499..2682
Gene:HK1
Gene Synonym:
Exon (20)2683..2838
Gene:HK1
Gene Synonym:
Exon (21)2839..3072
Gene:HK1
Gene Synonym:
Exon (22)3073..3964
Gene:HK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_033500
Organism Homo sapiens (human)
Definition Homo sapiens hexokinase 1 (HK1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033500

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCAAAAA GAGCCCTGCA TGATTTTATT GACAAGTATC TCTATGCCAT GCGGCTCTCC 
GATGAAACTC TCATAGATAT CATGACTCGC TTCAGGAAGG AGATGAAGAA TGGCCTCTCC
CGGGATTTTA ATCCAACAGC CACAGTCAAG ATGTTGCCAA CATTCGTAAG GTCCATTCCT
GATGGCTCTG AAAAGGGAGA TTTCATTGCC CTGGATCTTG GTGGGTCTTC CTTTCGAATT
CTGCGGGTGC AAGTGAATCA TGAGAAAAAC CAGAATGTTC ACATGGAGTC CGAGGTTTAT
GACACCCCAG AGAACATCGT GCACGGCAGT GGAAGCCAGC TTTTTGATCA TGTTGCTGAG
TGCCTGGGAG ATTTCATGGA GAAAAGGAAG ATCAAGGACA AGAAGTTACC TGTGGGATTC
ACGTTTTCTT TTCCTTGCCA ACAATCCAAA ATAGATGAGG CCATCCTGAT CACCTGGACA
AAGCGATTTA AAGCGAGCGG AGTGGAAGGA GCAGATGTGG TCAAACTGCT TAACAAAGCC
ATCAAAAAGC GAGGGGACTA TGATGCCAAC ATCGTAGCTG TGGTGAATGA CACAGTGGGC
ACCATGATGA CCTGTGGCTA TGACGACCAG CACTGTGAAG TCGGCCTGAT CATCGGCACT
GGCACCAATG CTTGCTACAT GGAGGAACTG AGGCACATTG ATCTGGTGGA AGGAGACGAG
GGGAGGATGT GTATCAATAC AGAATGGGGA GCCTTTGGAG ACGATGGATC ATTAGAAGAC
ATCCGGACAG AGTTTGACAG GGAGATAGAC CGGGGATCCC TCAACCCTGG AAAACAGCTG
TTTGAGAAGA TGGTCAGTGG CATGTACTTG GGAGAGCTGG TTCGACTGAT CCTAGTCAAG
ATGGCCAAGG AGGGCCTCTT ATTTGAAGGG CGGATCACCC CGGAGCTGCT CACCCGAGGG
AAGTTTAACA CCAGTGATGT GTCAGCCATC GAAAAGAATA AGGAAGGCCT CCACAATGCC
AAAGAAATCC TGACCCGCCT GGGAGTGGAG CCGTCCGATG ATGACTGTGT CTCAGTCCAG
CACGTTTGCA CCATTGTCTC ATTTCGCTCA GCCAACTTGG TGGCTGCCAC ACTGGGCGCC
ATCTTGAACC GCCTGCGTGA TAACAAGGGC ACACCCAGGC TGCGGACCAC GGTTGGTGTC
GACGGATCTC TTTACAAGAC GCACCCACAG TATTCCCGGC GTTTCCACAA GACTCTAAGG
CGCTTGGTGC CAGACTCCGA TGTGCGCTTC CTCCTCTCGG AGAGTGGCAG CGGCAAGGGG
GCTGCCATGG TGACGGCGGT GGCCTACCGC TTGGCCGAGC AGCACCGGCA GATAGAGGAG
ACCCTGGCTC ATTTCCACCT CACCAAGGAC ATGCTGCTGG AGGTGAAGAA GAGGATGCGG
GCCGAGATGG AGCTGGGGCT GAGGAAGCAG ACGCACAACA ATGCCGTGGT TAAGATGCTG
CCCTCCTTCG TCCGGAGAAC TCCCGACGGG ACCGAGAATG GTGACTTCTT GGCCCTGGAT
CTTGGAGGAA CCAATTTCCG TGTGCTGCTG GTGAAAATCC GTAGTGGGAA AAAGAGAACG
GTGGAAATGC ACAACAAGAT CTACGCCATT CCTATTGAAA TCATGCAGGG CACTGGGGAA
GAGCTGTTTG ATCACATTGT CTCCTGCATC TCTGACTTCT TGGACTACAT GGGGATCAAA
GGCCCCAGGA TGCCTCTGGG CTTCACGTTC TCATTTCCCT GCCAGCAGAC GAGTCTGGAC
GCGGGAATCT TGATCACGTG GACAAAGGGT TTTAAGGCAA CAGACTGCGT GGGCCACGAT
GTAGTCACCT TACTAAGGGA TGCGATAAAA AGGAGAGAGG AATTTGACCT GGACGTGGTG
GCTGTGGTCA ACGACACAGT GGGCACCATG ATGACCTGTG CTTATGAGGA GCCCACCTGT
GAGGTTGGAC TCATTGTTGG GACCGGCAGC AATGCCTGCT ACATGGAGGA GATGAAGAAC
GTGGAGATGG TGGAGGGGGA CCAGGGGCAG ATGTGCATCA ACATGGAGTG GGGGGCCTTT
GGGGACAACG GGTGTCTGGA TGATATCAGG ACACACTACG ACAGACTGGT GGACGAATAT
TCCCTAAATG CTGGGAAACA AAGGTATGAG AAGATGATCA GTGGTATGTA CCTGGGTGAA
ATCGTCCGCA ACATCTTAAT CGACTTCACC AAGAAGGGAT TCCTCTTCCG AGGGCAGATC
TCTGAGACGC TGAAGACCCG GGGCATCTTT GAGACCAAGT TTCTCTCTCA GATCGAGAGT
GACCGATTAG CACTGCTCCA GGTCCGGGCT ATCCTCCAGC AGCTAGGTCT GAATAGCACC
TGCGATGACA GTATCCTCGT CAAGACAGTG TGCGGGGTGG TGTCCAGGAG GGCCGCACAG
CTGTGTGGCG CAGGCATGGC TGCGGTTGTG GATAAGATCC GCGAGAACAG AGGACTGGAC
CGTCTGAATG TGACTGTGGG AGTGGACGGG ACACTCTACA AGCTTCATCC ACACTTCTCC
AGAATCATGC ACCAGACGGT GAAGGAACTG TCACCAAAAT GTAACGTGTC CTTCCTCCTG
TCTGAGGATG GCAGCGGCAA GGGGGCCGCC CTCATCACGG CCGTGGGCGT GCGGTTACGC
ACAGAGGCAA GCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09664
Clone ID Related Accession (Same CDS sequence) NM_033497 , NM_033498 , XM_005269736
Accession Version NM_033498.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform HKI-ta/tb
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U38226.1, AF073786.2, AK128226.1 and BC008730.2. On May 16, 2008 this sequence version replaced gi:15991830. Transcript Variant: Variant 4 (HKI-tb) has four testis-specific exons in the 5' end, one of which includes an additional 54 nt fragment unique to variants 4 and 5. Both variants 3 and 4 encode isoform HKI-ta/tb, which has a unique N-terminus. Isoform HKI-ta/tb lacks the porin binding domain (PBD) required for association with the mitochondrial membrane. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
AGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_277033.1
CDS359..3124
Misc Feature(1)311..313(+)
Misc Feature(2)404..1750(+)
Misc Feature(3)428..601(+)
Misc Feature(4)611..1156(+)
Misc Feature(5)620..1066(+)
Misc Feature(6)1037..1750(+)
Misc Feature(7)1754..3091(+)
Misc Feature(8)1955..>2446(+)
Misc Feature(9)1964..2410(+)
Misc Feature(10)2384..3091(+)
Exon (1)1..109
Gene:HK1
Gene Synonym:
Exon (2)110..285
Gene:HK1
Gene Synonym:
Exon (3)286..385
Gene:HK1
Gene Synonym:
Exon (4)386..433
Gene:HK1
Gene Synonym:
Exon (5)434..596
Gene:HK1
Gene Synonym:
Exon (6)597..745
Gene:HK1
Gene Synonym:
Exon (7)746..865
Gene:HK1
Gene Synonym:
Exon (8)866..961
Gene:HK1
Gene Synonym:
Exon (9)962..1061
Gene:HK1
Gene Synonym:
Exon (10)1062..1245
Gene:HK1
Gene Synonym:
Exon (11)1246..1401
Gene:HK1
Gene Synonym:
Exon (12)1402..1635
Gene:HK1
Gene Synonym:
Exon (13)1636..1940
Gene:HK1
Gene Synonym:
Exon (14)1941..2089
Gene:HK1
Gene Synonym:
Exon (15)2090..2209
Gene:HK1
Gene Synonym:
Exon (16)2210..2305
Gene:HK1
Gene Synonym:
Exon (17)2306..2405
Gene:HK1
Gene Synonym:
Exon (18)2406..2589
Gene:HK1
Gene Synonym:
Exon (19)2590..2745
Gene:HK1
Gene Synonym:
Exon (20)2746..2979
Gene:HK1
Gene Synonym:
Exon (21)2980..3871
Gene:HK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_033498
Organism Homo sapiens (human)
Definition Homo sapiens hexokinase 1 (HK1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033498

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
AGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09664
Clone ID Related Accession (Same CDS sequence) NM_033497 , NM_033498 , XM_005269736
Accession Version NM_033497.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform HKI-ta/tb
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB088955.1, AF073786.2, AK128226.1 and BC008730.2. On May 16, 2008 this sequence version replaced gi:15991828. Transcript Variant: Variant 3 (HKI-ta) includes four testis-specific exons in the 5' end. Both variants 3 and 4 encode isoform HKI-ta/tb, which has a unique N-terminus. Isoform HKI-ta/tb lacks the porin binding domain (PBD) required for association with the mitochondrial membrane. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
AGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_277032.1
CDS305..3070
Misc Feature(1)257..259(+)
Misc Feature(2)350..1696(+)
Misc Feature(3)374..547(+)
Misc Feature(4)557..1102(+)
Misc Feature(5)566..1012(+)
Misc Feature(6)983..1696(+)
Misc Feature(7)1700..3037(+)
Misc Feature(8)1901..>2392(+)
Misc Feature(9)1910..2356(+)
Misc Feature(10)2330..3037(+)
Exon (1)1..109
Gene:HK1
Gene Synonym:
Exon (2)110..231
Gene:HK1
Gene Synonym:
Exon (3)232..331
Gene:HK1
Gene Synonym:
Exon (4)332..379
Gene:HK1
Gene Synonym:
Exon (5)380..542
Gene:HK1
Gene Synonym:
Exon (6)543..691
Gene:HK1
Gene Synonym:
Exon (7)692..811
Gene:HK1
Gene Synonym:
Exon (8)812..907
Gene:HK1
Gene Synonym:
Exon (9)908..1007
Gene:HK1
Gene Synonym:
Exon (10)1008..1191
Gene:HK1
Gene Synonym:
Exon (11)1192..1347
Gene:HK1
Gene Synonym:
Exon (12)1348..1581
Gene:HK1
Gene Synonym:
Exon (13)1582..1886
Gene:HK1
Gene Synonym:
Exon (14)1887..2035
Gene:HK1
Gene Synonym:
Exon (15)2036..2155
Gene:HK1
Gene Synonym:
Exon (16)2156..2251
Gene:HK1
Gene Synonym:
Exon (17)2252..2351
Gene:HK1
Gene Synonym:
Exon (18)2352..2535
Gene:HK1
Gene Synonym:
Exon (19)2536..2691
Gene:HK1
Gene Synonym:
Exon (20)2692..2925
Gene:HK1
Gene Synonym:
Exon (21)2926..3817
Gene:HK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_033497
Organism Homo sapiens (human)
Definition Homo sapiens hexokinase 1 (HK1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033497

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGGGCAGA TCTGCCAGCG AGAATCGGCT ACAGCAGCTG AAAAACCAAA ACTTCATCTA 
CTTGCTGAAA GTGAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCCGA TGAAACTCTC
ATAGATATCA TGACTCGCTT CAGGAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT
CCAACAGCCA CAGTCAAGAT GTTGCCAACA TTCGTAAGGT CCATTCCTGA TGGCTCTGAA
AAGGGAGATT TCATTGCCCT GGATCTTGGT GGGTCTTCCT TTCGAATTCT GCGGGTGCAA
GTGAATCATG AGAAAAACCA GAATGTTCAC ATGGAGTCCG AGGTTTATGA CACCCCAGAG
AACATCGTGC ACGGCAGTGG AAGCCAGCTT TTTGATCATG TTGCTGAGTG CCTGGGAGAT
TTCATGGAGA AAAGGAAGAT CAAGGACAAG AAGTTACCTG TGGGATTCAC GTTTTCTTTT
CCTTGCCAAC AATCCAAAAT AGATGAGGCC ATCCTGATCA CCTGGACAAA GCGATTTAAA
GCGAGCGGAG TGGAAGGAGC AGATGTGGTC AAACTGCTTA ACAAAGCCAT CAAAAAGCGA
GGGGACTATG ATGCCAACAT CGTAGCTGTG GTGAATGACA CAGTGGGCAC CATGATGACC
TGTGGCTATG ACGACCAGCA CTGTGAAGTC GGCCTGATCA TCGGCACTGG CACCAATGCT
TGCTACATGG AGGAACTGAG GCACATTGAT CTGGTGGAAG GAGACGAGGG GAGGATGTGT
ATCAATACAG AATGGGGAGC CTTTGGAGAC GATGGATCAT TAGAAGACAT CCGGACAGAG
TTTGACAGGG AGATAGACCG GGGATCCCTC AACCCTGGAA AACAGCTGTT TGAGAAGATG
GTCAGTGGCA TGTACTTGGG AGAGCTGGTT CGACTGATCC TAGTCAAGAT GGCCAAGGAG
GGCCTCTTAT TTGAAGGGCG GATCACCCCG GAGCTGCTCA CCCGAGGGAA GTTTAACACC
AGTGATGTGT CAGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG
ACCCGCCTGG GAGTGGAGCC GTCCGATGAT GACTGTGTCT CAGTCCAGCA CGTTTGCACC
ATTGTCTCAT TTCGCTCAGC CAACTTGGTG GCTGCCACAC TGGGCGCCAT CTTGAACCGC
CTGCGTGATA ACAAGGGCAC ACCCAGGCTG CGGACCACGG TTGGTGTCGA CGGATCTCTT
TACAAGACGC ACCCACAGTA TTCCCGGCGT TTCCACAAGA CTCTAAGGCG CTTGGTGCCA
GACTCCGATG TGCGCTTCCT CCTCTCGGAG AGTGGCAGCG GCAAGGGGGC TGCCATGGTG
ACGGCGGTGG CCTACCGCTT GGCCGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCTCAT
TTCCACCTCA CCAAGGACAT GCTGCTGGAG GTGAAGAAGA GGATGCGGGC CGAGATGGAG
CTGGGGCTGA GGAAGCAGAC GCACAACAAT GCCGTGGTTA AGATGCTGCC CTCCTTCGTC
CGGAGAACTC CCGACGGGAC CGAGAATGGT GACTTCTTGG CCCTGGATCT TGGAGGAACC
AATTTCCGTG TGCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGAACGGT GGAAATGCAC
AACAAGATCT ACGCCATTCC TATTGAAATC ATGCAGGGCA CTGGGGAAGA GCTGTTTGAT
CACATTGTCT CCTGCATCTC TGACTTCTTG GACTACATGG GGATCAAAGG CCCCAGGATG
CCTCTGGGCT TCACGTTCTC ATTTCCCTGC CAGCAGACGA GTCTGGACGC GGGAATCTTG
ATCACGTGGA CAAAGGGTTT TAAGGCAACA GACTGCGTGG GCCACGATGT AGTCACCTTA
CTAAGGGATG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ACGTGGTGGC TGTGGTCAAC
GACACAGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGTGA GGTTGGACTC
ATTGTTGGGA CCGGCAGCAA TGCCTGCTAC ATGGAGGAGA TGAAGAACGT GGAGATGGTG
GAGGGGGACC AGGGGCAGAT GTGCATCAAC ATGGAGTGGG GGGCCTTTGG GGACAACGGG
TGTCTGGATG ATATCAGGAC ACACTACGAC AGACTGGTGG ACGAATATTC CCTAAATGCT
GGGAAACAAA GGTATGAGAA GATGATCAGT GGTATGTACC TGGGTGAAAT CGTCCGCAAC
ATCTTAATCG ACTTCACCAA GAAGGGATTC CTCTTCCGAG GGCAGATCTC TGAGACGCTG
AAGACCCGGG GCATCTTTGA GACCAAGTTT CTCTCTCAGA TCGAGAGTGA CCGATTAGCA
CTGCTCCAGG TCCGGGCTAT CCTCCAGCAG CTAGGTCTGA ATAGCACCTG CGATGACAGT
ATCCTCGTCA AGACAGTGTG CGGGGTGGTG TCCAGGAGGG CCGCACAGCT GTGTGGCGCA
GGCATGGCTG CGGTTGTGGA TAAGATCCGC GAGAACAGAG GACTGGACCG TCTGAATGTG
ACTGTGGGAG TGGACGGGAC ACTCTACAAG CTTCATCCAC ACTTCTCCAG AATCATGCAC
CAGACGGTGA AGGAACTGTC ACCAAAATGT AACGTGTCCT TCCTCCTGTC TGAGGATGGC
AGCGGCAAGG GGGCCGCCCT CATCACGGCC GTGGGCGTGC GGTTACGCAC AGAGGCAAGC
AGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10118
Clone ID Related Accession (Same CDS sequence) NM_000188
Accession Version NM_000188.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hexokinase-1 isoform HKI
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292012.1, AB209526.1, AK128226.1 and BC008730.2. On May 16, 2008 this sequence version replaced gi:4504390. Transcript Variant: Variant 1 (HKI) encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC008730.2, AB209526.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGATCGCCG CGCAGCTCCT GGCCTATTAC TTCACGGAGC TGAAGGATGA CCAGGTCAAA 
AAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCCGATG AAACTCTCAT AGATATCATG
ACTCGCTTCA GGAAGGAGAT GAAGAATGGC CTCTCCCGGG ATTTTAATCC AACAGCCACA
GTCAAGATGT TGCCAACATT CGTAAGGTCC ATTCCTGATG GCTCTGAAAA GGGAGATTTC
ATTGCCCTGG ATCTTGGTGG GTCTTCCTTT CGAATTCTGC GGGTGCAAGT GAATCATGAG
AAAAACCAGA ATGTTCACAT GGAGTCCGAG GTTTATGACA CCCCAGAGAA CATCGTGCAC
GGCAGTGGAA GCCAGCTTTT TGATCATGTT GCTGAGTGCC TGGGAGATTT CATGGAGAAA
AGGAAGATCA AGGACAAGAA GTTACCTGTG GGATTCACGT TTTCTTTTCC TTGCCAACAA
TCCAAAATAG ATGAGGCCAT CCTGATCACC TGGACAAAGC GATTTAAAGC GAGCGGAGTG
GAAGGAGCAG ATGTGGTCAA ACTGCTTAAC AAAGCCATCA AAAAGCGAGG GGACTATGAT
GCCAACATCG TAGCTGTGGT GAATGACACA GTGGGCACCA TGATGACCTG TGGCTATGAC
GACCAGCACT GTGAAGTCGG CCTGATCATC GGCACTGGCA CCAATGCTTG CTACATGGAG
GAACTGAGGC ACATTGATCT GGTGGAAGGA GACGAGGGGA GGATGTGTAT CAATACAGAA
TGGGGAGCCT TTGGAGACGA TGGATCATTA GAAGACATCC GGACAGAGTT TGACAGGGAG
ATAGACCGGG GATCCCTCAA CCCTGGAAAA CAGCTGTTTG AGAAGATGGT CAGTGGCATG
TACTTGGGAG AGCTGGTTCG ACTGATCCTA GTCAAGATGG CCAAGGAGGG CCTCTTATTT
GAAGGGCGGA TCACCCCGGA GCTGCTCACC CGAGGGAAGT TTAACACCAG TGATGTGTCA
GCCATCGAAA AGAATAAGGA AGGCCTCCAC AATGCCAAAG AAATCCTGAC CCGCCTGGGA
GTGGAGCCGT CCGATGATGA CTGTGTCTCA GTCCAGCACG TTTGCACCAT TGTCTCATTT
CGCTCAGCCA ACTTGGTGGC TGCCACACTG GGCGCCATCT TGAACCGCCT GCGTGATAAC
AAGGGCACAC CCAGGCTGCG GACCACGGTT GGTGTCGACG GATCTCTTTA CAAGACGCAC
CCACAGTATT CCCGGCGTTT CCACAAGACT CTAAGGCGCT TGGTGCCAGA CTCCGATGTG
CGCTTCCTCC TCTCGGAGAG TGGCAGCGGC AAGGGGGCTG CCATGGTGAC GGCGGTGGCC
TACCGCTTGG CCGAGCAGCA CCGGCAGATA GAGGAGACCC TGGCTCATTT CCACCTCACC
AAGGACATGC TGCTGGAGGT GAAGAAGAGG ATGCGGGCCG AGATGGAGCT GGGGCTGAGG
AAGCAGACGC ACAACAATGC CGTGGTTAAG ATGCTGCCCT CCTTCGTCCG GAGAACTCCC
GACGGGACCG AGAATGGTGA CTTCTTGGCC CTGGATCTTG GAGGAACCAA TTTCCGTGTG
CTGCTGGTGA AAATCCGTAG TGGGAAAAAG AGAACGGTGG AAATGCACAA CAAGATCTAC
GCCATTCCTA TTGAAATCAT GCAGGGCACT GGGGAAGAGC TGTTTGATCA CATTGTCTCC
TGCATCTCTG ACTTCTTGGA CTACATGGGG ATCAAAGGCC CCAGGATGCC TCTGGGCTTC
ACGTTCTCAT TTCCCTGCCA GCAGACGAGT CTGGACGCGG GAATCTTGAT CACGTGGACA
AAGGGTTTTA AGGCAACAGA CTGCGTGGGC CACGATGTAG TCACCTTACT AAGGGATGCG
ATAAAAAGGA GAGAGGAATT TGACCTGGAC GTGGTGGCTG TGGTCAACGA CACAGTGGGC
ACCATGATGA CCTGTGCTTA TGAGGAGCCC ACCTGTGAGG TTGGACTCAT TGTTGGGACC
GGCAGCAATG CCTGCTACAT GGAGGAGATG AAGAACGTGG AGATGGTGGA GGGGGACCAG
GGGCAGATGT GCATCAACAT GGAGTGGGGG GCCTTTGGGG ACAACGGGTG TCTGGATGAT
ATCAGGACAC ACTACGACAG ACTGGTGGAC GAATATTCCC TAAATGCTGG GAAACAAAGG
TATGAGAAGA TGATCAGTGG TATGTACCTG GGTGAAATCG TCCGCAACAT CTTAATCGAC
TTCACCAAGA AGGGATTCCT CTTCCGAGGG CAGATCTCTG AGACGCTGAA GACCCGGGGC
ATCTTTGAGA CCAAGTTTCT CTCTCAGATC GAGAGTGACC GATTAGCACT GCTCCAGGTC
CGGGCTATCC TCCAGCAGCT AGGTCTGAAT AGCACCTGCG ATGACAGTAT CCTCGTCAAG
ACAGTGTGCG GGGTGGTGTC CAGGAGGGCC GCACAGCTGT GTGGCGCAGG CATGGCTGCG
GTTGTGGATA AGATCCGCGA GAACAGAGGA CTGGACCGTC TGAATGTGAC TGTGGGAGTG
GACGGGACAC TCTACAAGCT TCATCCACAC TTCTCCAGAA TCATGCACCA GACGGTGAAG
GAACTGTCAC CAAAATGTAA CGTGTCCTTC CTCCTGTCTG AGGATGGCAG CGGCAAGGGG
GCCGCCCTCA TCACGGCCGT GGGCGTGCGG TTACGCACAG AGGCAAGCAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000179.2
CDS102..2855
Misc Feature(1)102..137(+)
Misc Feature(2)102..104(+)
Misc Feature(3)135..1481(+)
Misc Feature(4)138..1526(+)
Misc Feature(5)342..887(+)
Misc Feature(6)351..797(+)
Misc Feature(7)351..365(+)
Misc Feature(8)615..620(+)
Misc Feature(9)723..728(+)
Misc Feature(10)768..1481(+)
Misc Feature(11)972..983(+)
Misc Feature(12)1338..1346(+)
Misc Feature(13)1485..2822(+)
Misc Feature(14)1527..2852(+)
Misc Feature(15)1686..>2177(+)
Misc Feature(16)1695..2141(+)
Misc Feature(17)1695..1709(+)
Misc Feature(18)1908..1913(+)
Misc Feature(19)1959..1964(+)
Misc Feature(20)2067..2072(+)
Misc Feature(21)2115..2822(+)
Misc Feature(22)2145..2150(+)
Misc Feature(23)2682..2690(+)
Exon (1)1..164
Gene:HK1
Gene Synonym:
Exon (2)165..327
Gene:HK1
Gene Synonym:
Exon (3)328..476
Gene:HK1
Gene Synonym:
Exon (4)477..596
Gene:HK1
Gene Synonym:
Exon (5)597..692
Gene:HK1
Gene Synonym:
Exon (6)693..792
Gene:HK1
Gene Synonym:
Exon (7)793..976
Gene:HK1
Gene Synonym:
Exon (8)977..1132
Gene:HK1
Gene Synonym:
Exon (9)1133..1366
Gene:HK1
Gene Synonym:
Exon (10)1367..1671
Gene:HK1
Gene Synonym:
Exon (11)1672..1820
Gene:HK1
Gene Synonym:
Exon (12)1821..1940
Gene:HK1
Gene Synonym:
Exon (13)1941..2036
Gene:HK1
Gene Synonym:
Exon (14)2037..2136
Gene:HK1
Gene Synonym:
Exon (15)2137..2320
Gene:HK1
Gene Synonym:
Exon (16)2321..2476
Gene:HK1
Gene Synonym:
Exon (17)2477..2710
Gene:HK1
Gene Synonym:
Exon (18)2711..3602
Gene:HK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000188
Organism Homo sapiens (human)
Definition Homo sapiens hexokinase 1 (HK1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000188

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGATCGCCG CGCAGCTCCT GGCCTATTAC TTCACGGAGC TGAAGGATGA CCAGGTCAAA 
AAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCCGATG AAACTCTCAT AGATATCATG
ACTCGCTTCA GGAAGGAGAT GAAGAATGGC CTCTCCCGGG ATTTTAATCC AACAGCCACA
GTCAAGATGT TGCCAACATT CGTAAGGTCC ATTCCTGATG GCTCTGAAAA GGGAGATTTC
ATTGCCCTGG ATCTTGGTGG GTCTTCCTTT CGAATTCTGC GGGTGCAAGT GAATCATGAG
AAAAACCAGA ATGTTCACAT GGAGTCCGAG GTTTATGACA CCCCAGAGAA CATCGTGCAC
GGCAGTGGAA GCCAGCTTTT TGATCATGTT GCTGAGTGCC TGGGAGATTT CATGGAGAAA
AGGAAGATCA AGGACAAGAA GTTACCTGTG GGATTCACGT TTTCTTTTCC TTGCCAACAA
TCCAAAATAG ATGAGGCCAT CCTGATCACC TGGACAAAGC GATTTAAAGC GAGCGGAGTG
GAAGGAGCAG ATGTGGTCAA ACTGCTTAAC AAAGCCATCA AAAAGCGAGG GGACTATGAT
GCCAACATCG TAGCTGTGGT GAATGACACA GTGGGCACCA TGATGACCTG TGGCTATGAC
GACCAGCACT GTGAAGTCGG CCTGATCATC GGCACTGGCA CCAATGCTTG CTACATGGAG
GAACTGAGGC ACATTGATCT GGTGGAAGGA GACGAGGGGA GGATGTGTAT CAATACAGAA
TGGGGAGCCT TTGGAGACGA TGGATCATTA GAAGACATCC GGACAGAGTT TGACAGGGAG
ATAGACCGGG GATCCCTCAA CCCTGGAAAA CAGCTGTTTG AGAAGATGGT CAGTGGCATG
TACTTGGGAG AGCTGGTTCG ACTGATCCTA GTCAAGATGG CCAAGGAGGG CCTCTTATTT
GAAGGGCGGA TCACCCCGGA GCTGCTCACC CGAGGGAAGT TTAACACCAG TGATGTGTCA
GCCATCGAAA AGAATAAGGA AGGCCTCCAC AATGCCAAAG AAATCCTGAC CCGCCTGGGA
GTGGAGCCGT CCGATGATGA CTGTGTCTCA GTCCAGCACG TTTGCACCAT TGTCTCATTT
CGCTCAGCCA ACTTGGTGGC TGCCACACTG GGCGCCATCT TGAACCGCCT GCGTGATAAC
AAGGGCACAC CCAGGCTGCG GACCACGGTT GGTGTCGACG GATCTCTTTA CAAGACGCAC
CCACAGTATT CCCGGCGTTT CCACAAGACT CTAAGGCGCT TGGTGCCAGA CTCCGATGTG
CGCTTCCTCC TCTCGGAGAG TGGCAGCGGC AAGGGGGCTG CCATGGTGAC GGCGGTGGCC
TACCGCTTGG CCGAGCAGCA CCGGCAGATA GAGGAGACCC TGGCTCATTT CCACCTCACC
AAGGACATGC TGCTGGAGGT GAAGAAGAGG ATGCGGGCCG AGATGGAGCT GGGGCTGAGG
AAGCAGACGC ACAACAATGC CGTGGTTAAG ATGCTGCCCT CCTTCGTCCG GAGAACTCCC
GACGGGACCG AGAATGGTGA CTTCTTGGCC CTGGATCTTG GAGGAACCAA TTTCCGTGTG
CTGCTGGTGA AAATCCGTAG TGGGAAAAAG AGAACGGTGG AAATGCACAA CAAGATCTAC
GCCATTCCTA TTGAAATCAT GCAGGGCACT GGGGAAGAGC TGTTTGATCA CATTGTCTCC
TGCATCTCTG ACTTCTTGGA CTACATGGGG ATCAAAGGCC CCAGGATGCC TCTGGGCTTC
ACGTTCTCAT TTCCCTGCCA GCAGACGAGT CTGGACGCGG GAATCTTGAT CACGTGGACA
AAGGGTTTTA AGGCAACAGA CTGCGTGGGC CACGATGTAG TCACCTTACT AAGGGATGCG
ATAAAAAGGA GAGAGGAATT TGACCTGGAC GTGGTGGCTG TGGTCAACGA CACAGTGGGC
ACCATGATGA CCTGTGCTTA TGAGGAGCCC ACCTGTGAGG TTGGACTCAT TGTTGGGACC
GGCAGCAATG CCTGCTACAT GGAGGAGATG AAGAACGTGG AGATGGTGGA GGGGGACCAG
GGGCAGATGT GCATCAACAT GGAGTGGGGG GCCTTTGGGG ACAACGGGTG TCTGGATGAT
ATCAGGACAC ACTACGACAG ACTGGTGGAC GAATATTCCC TAAATGCTGG GAAACAAAGG
TATGAGAAGA TGATCAGTGG TATGTACCTG GGTGAAATCG TCCGCAACAT CTTAATCGAC
TTCACCAAGA AGGGATTCCT CTTCCGAGGG CAGATCTCTG AGACGCTGAA GACCCGGGGC
ATCTTTGAGA CCAAGTTTCT CTCTCAGATC GAGAGTGACC GATTAGCACT GCTCCAGGTC
CGGGCTATCC TCCAGCAGCT AGGTCTGAAT AGCACCTGCG ATGACAGTAT CCTCGTCAAG
ACAGTGTGCG GGGTGGTGTC CAGGAGGGCC GCACAGCTGT GTGGCGCAGG CATGGCTGCG
GTTGTGGATA AGATCCGCGA GAACAGAGGA CTGGACCGTC TGAATGTGAC TGTGGGAGTG
GACGGGACAC TCTACAAGCT TCATCCACAC TTCTCCAGAA TCATGCACCA GACGGTGAAG
GAACTGTCAC CAAAATGTAA CGTGTCCTTC CTCCTGTCTG AGGATGGCAG CGGCAAGGGG
GCCGCCCTCA TCACGGCCGT GGGCGTGCGG TTACGCACAG AGGCAAGCAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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