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ARF5 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol ARF5
Entrez Gene ID 381
Full Name ADP ribosylation factor 5
General protein information
Preferred Names

ADP ribosylation factor 5

Names

ADP-ribosylation factor 5

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7q32.1

Summary This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010].
Disorder MIM:

103188


mRNA and Protein(s)

mRNA Protein Name
NM_001662.3 NP_001653.1 ADP-ribosylation factor 5



Pan troglodytes (chimpanzee) ARF5 XP_519351.3
Bos taurus (cattle) ARF5 NP_001077140.1
Danio rerio (zebrafish) arf5 NP_954969.1
Homo sapiens (human) ARF5 NP_001653.1
Rattus norvegicus (Norway rat) Arf5 NP_077063.1
Danio rerio (zebrafish) zgc:77650 NP_956170.1
Mus musculus (house mouse) Arf5 NP_031506.1
Caenorhabditis elegans (roundworm) arf-3 NP_501336.1
Canis lupus familiaris (dog) ARF5 XP_532438.3


Related articles in PubMed

GORAB Missense Mutations Disrupt RAB6 and ARF5 Binding and Golgi Targeting.
Egerer J, Emmerich D, Fischer-Zirnsak B, Chan WL, Meierhofer D, Tuysuz B, Marschner K, Sauer S, Barr FA, Mundlos S, Kornak U
The Journal of investigative dermatology135(10)2368-2376(2015 Oct)

WLS retrograde transport to the endoplasmic reticulum during Wnt secretion.
Yu J, Chia J, Canning CA, Jones CM, Bard FA, Virshup DM
Developmental cell29(3)277-91(2014 May)

Genome-wide association study in a Chinese population identifies a susceptibility locus for type 2 diabetes at 7q32 near PAX4.
Ma RC, Hu C, Tam CH, Zhang R, Kwan P, Leung TF, Thomas GN, Go MJ, Hara K, Sim X, Ho JS, Wang C, Li H, Lu L, Wang Y, Li JW, Wang Y, Lam VK, Wang J, Yu W, Kim YJ, Ng DP, Fujita H, Panoutsopoulou K, Day-Williams AG, Lee HM, Ng AC, Fang YJ, Kong AP, Jiang F, Ma X, Hou X, Tang S, Lu J, Yamauchi T, Tsui SK, Woo J, Leung PC, Zhang X, Tang NL, Sy HY, Liu J, Wong TY, Lee JY, Maeda S, Xu G, Cherny SS, Chan TF, Ng MC, Xiang K, Morris AP, , Keildson S, , Hu R, Ji L, Lin X, Cho YS, Kadowaki T, Tai ES, Zeggini E, McCarthy MI, Hon KL, Baum L, Tomlinson B, So WY, Bao Y, Chan JC, Jia W
Diabetologia56(6)1291-305(2013 Jun)

BRAG2/GEP100/IQSec1 interacts with clathrin and regulates α5β1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5).
Moravec R, Conger KK, D'Souza R, Allison AB, Casanova JE
The Journal of biological chemistry287(37)31138-47(2012 Sep)

ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5.
Hasegawa J, Tsujita K, Takenawa T, Itoh T
Molecular biology of the cell23(13)2481-9(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GORAB missense mutation disrupt ARF5 binding in Golgi apparatus in patients, with genetic skin diseases.
Title: GORAB Missense Mutations Disrupt RAB6 and ARF5 Binding and Golgi Targeting.

BRAG2 acts at clathrin-coated pits to promote integrin internalization by activating Arf5 and suggest a previously unrecognized role for Arf5 in clathrin-mediated endocytosis of specific cargoes.
Title: BRAG2/GEP100/IQSec1 interacts with clathrin and regulates α5β1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5).

a novel molecular mechanism of circular dorsal ruffles ring size control through the ARAP1-Arf1/5 pathway.
Title: ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5.

A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration.
Title: Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.

The lipid droplets deposition and the cellular triacylglycerol content are significantly increased by siRNA-mediated depletion of Arf5.
Title: GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis.

The following ARF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu01577 NM_001662.3
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Homo sapiens ADP ribosylation factor 5 (ARF5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu01577
Clone ID Related Accession (Same CDS sequence) NM_001662.3
Accession Version NM_001662.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 543bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product ADP-ribosylation factor 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN259625.1 and AC000357.1. On Dec 9, 2010 this sequence version replaced NM_001662.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033104.1, AL545339.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
ATGGGCCTCA CCGTGTCCGC GCTCTTTTCG CGGATCTTCG GGAAGAAGCA GATGCGGATT 
CTCATGGTTG GCTTGGATGC GGCTGGCAAG ACCACAATCC TGTACAAACT GAAGTTGGGG
GAGATTGTCA CCACCATCCC AACCATAGGC TTCAATGTAG AAACAGTGGA ATATAAGAAC
ATCTGTTTCA CAGTCTGGGA CGTGGGAGGC CAGGACAAGA TTCGGCCTCT GTGGCGGCAC
TACTTCCAGA ACACTCAGGG CCTCATCTTT GTGGTGGACA GTAATGACCG GGAGCGGGTC
CAAGAATCTG CTGATGAACT CCAGAAGATG CTGCAGGAGG ACGAGCTGCG GGATGCAGTG
CTGCTGGTAT TTGCCAACAA GCAGGACATG CCCAACGCCA TGCCCGTGAG CGAGCTGACT
GACAAGCTGG GGCTACAGCA CTTACGCAGC CGCACGTGGT ATGTCCAGGC CACCTGTGCC
ACCCAAGGCA CAGGTCTGTA CGATGGTCTG GACTGGCTGT CCCACGAGCT GTCAAAGCGC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001653.1
CDS148..690
Misc Feature(1)148..687
Misc Feature(2)184..>618
Misc Feature(3)217..240
Misc Feature(4)order(223..243,523..528,532..534,622..630)
Misc Feature(5)order(223..228,238..240,250..252,286..309,373..375,388..390)
Misc Feature(6)253..300
Misc Feature(7)order(289..303,316..318,346..348,358..360,364..366,376..378,382..390)
Misc Feature(8)289..291
Misc Feature(9)order(292..300,364..366,385..387)
Misc Feature(10)298..345
Misc Feature(11)346..357
Misc Feature(12)352..405
Misc Feature(13)523..534
Misc Feature(14)622..630
Exon (1)1..214
Exon (2)215..295
Exon (3)296..405
Exon (4)406..477
Exon (5)478..603
Exon (6)604..1096
Translation

Target ORF information:

RefSeq Version NM_001662.3
Organism Homo sapiens(Human)
Definition Homo sapiens ADP ribosylation factor 5 (ARF5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001662.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCCTCA CCGTGTCCGC GCTCTTTTCG CGGATCTTCG GGAAGAAGCA GATGCGGATT 
CTCATGGTTG GCTTGGATGC GGCTGGCAAG ACCACAATCC TGTACAAACT GAAGTTGGGG
GAGATTGTCA CCACCATCCC AACCATAGGC TTCAATGTAG AAACAGTGGA ATATAAGAAC
ATCTGTTTCA CAGTCTGGGA CGTGGGAGGC CAGGACAAGA TTCGGCCTCT GTGGCGGCAC
TACTTCCAGA ACACTCAGGG CCTCATCTTT GTGGTGGACA GTAATGACCG GGAGCGGGTC
CAAGAATCTG CTGATGAACT CCAGAAGATG CTGCAGGAGG ACGAGCTGCG GGATGCAGTG
CTGCTGGTAT TTGCCAACAA GCAGGACATG CCCAACGCCA TGCCCGTGAG CGAGCTGACT
GACAAGCTGG GGCTACAGCA CTTACGCAGC CGCACGTGGT ATGTCCAGGC CACCTGTGCC
ACCCAAGGCA CAGGTCTGTA CGATGGTCTG GACTGGCTGT CCCACGAGCT GTCAAAGCGC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

GORAB Missense Mutations Disrupt RAB6 and ARF5 Binding and Golgi Targeting.
The Journal of investigative dermatology135(10)2368-2376(2015 Oct)
Egerer J,Emmerich D,Fischer-Zirnsak B,Chan WL,Meierhofer D,Tuysuz B,Marschner K,Sauer S,Barr FA,Mundlos S,Kornak U


book

WLS retrograde transport to the endoplasmic reticulum during Wnt secretion.
Developmental cell29(3)277-91(2014 May)
Yu J,Chia J,Canning CA,Jones CM,Bard FA,Virshup DM


book

Genome-wide association study in a Chinese population identifies a susceptibility locus for type 2 diabetes at 7q32 near PAX4.
Diabetologia56(6)1291-305(2013 Jun)
Ma RC,Hu C,Tam CH,Zhang R,Kwan P,Leung TF,Thomas GN,Go MJ,Hara K,Sim X,Ho JS,Wang C,Li H,Lu L,Wang Y,Li JW,Wang Y,Lam VK,Wang J,Yu W,Kim YJ,Ng DP,Fujita H,Panoutsopoulou K,Day-Williams AG,Lee HM,Ng AC,Fang YJ,Kong AP,Jiang F,Ma X,Hou X,Tang S,Lu J,Yamauchi T,Tsui SK,Woo J,Leung PC,Zhang X,Tang NL,Sy HY,Liu J,Wong TY,Lee JY,Maeda S,Xu G,Cherny SS,Chan TF,Ng MC,Xiang K,Morris AP,,Keildson S,,Hu R,Ji L,Lin X,Cho YS,Kadowaki T,Tai ES,Zeggini E,McCarthy MI,Hon KL,Baum L,Tomlinson B,So WY,Bao Y,Chan JC,Jia W


book

BRAG2/GEP100/IQSec1 interacts with clathrin and regulates α5β1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5).
The Journal of biological chemistry287(37)31138-47(2012 Sep)
Moravec R,Conger KK,D'Souza R,Allison AB,Casanova JE


book

ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5.
Molecular biology of the cell23(13)2481-9(2012 Jul)
Hasegawa J,Tsujita K,Takenawa T,Itoh T


 
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