NCL cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | NCL |
| Entrez Gene ID | 4691 |
| Full Name | nucleolin |
| Synonyms | C23,Nsr1 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]. |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_005381.2 | NP_005372.2 | nucleolin |
| Rattus norvegicus (Norway rat) | Ncl | NP_036881.2 |
| Canis lupus familiaris (dog) | NCL | XP_850477.2 |
| Mus musculus (house mouse) | Ncl | NP_035010.3 |
| Bos taurus (cattle) | NCL | NP_001193589.1 |
| Homo sapiens (human) | NCL | NP_005372.2 |
| Pan troglodytes (chimpanzee) | NCL | XP_516145.3 |
| LOC720965 | XP_001116949.2 |
Related articles in PubMed
Identification of Microprotein-Protein Interactions via APEX Tagging.
Chu Q, Rathore A, Diedrich JK, Donaldson CJ, Yates JR 3rd, Saghatelian A
Biochemistry56(26)3299-3306(2017 07)
Phorbol-12-myristate-13-acetate-mediated stabilization of leukemia inhibitory factor (
Chakraborty A, Mukherjee S, Saha S, De S, Sengupta Bandyopadhyay S
The Biochemical journal474(14)2349-2363(2017 07)
Nucleolin is a nuclear target of heparan sulfate derived from glypican-1.
Cheng F, Belting M, Fransson L?, Mani K
Experimental cell research354(1)31-39(2017 05)
Engineered antibody CH2 domains binding to nucleolin: Isolation, characterization and improvement of aggregation.
Li D, Gong R, Zheng J, Chen X, Dimitrov DS, Zhao Q
Biochemical and biophysical research communications485(2)446-453(2017 04)
Whole-exome sequencing of individuals from an isolated population implicates rare risk variants in bipolar disorder.
Lescai F, Als TD, Li Q, Nyegaard M, Andorsdottir G, Biskopst? M, Hedemand A, Fiorentino A, O'Brien N, Jarram A, Liang J, Grove J, Pallesen J, Eickhardt E, Mattheisen M, Bolund L, Demontis D, Wang AG, McQuillin A, Mors O, Wang J, B?rglum AD
Translational psychiatry7(2)e1034(2017 02)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Data suggest that C11orf98 microprotein, NPM1, and nucleolin interact and colocalize in the cell nucleolus. (C11orf98 = chromosome 11 open reading frame 98 protein; NPM1 = nucleophosmin 1)
Title: Identification of Microprotein-Protein Interactions via APEX Tagging.
Data suggest a 216-nucleotide proximal cis-element in LIF mRNA exhibits mRNA destabilizing potential; on exposure to carcinogen PMA (phorbol-12-myristate-13-acetate), this cis-element exhibits mRNA stabilizing activity. PMA induces nucleo-cytoplasmic translocation of both nucleolin and PCBP1, 2 trans-acting factors that bind to and stabilize LIF mRNA. [LIF = leukemia inhibitory factor; PCBP1 = poly(rC) binding protein 1]
Title: Phorbol-12-myristate-13-acetate-mediated stabilization of leukemia inhibitory factor (
A portion of HS-anMan colocalized with nucleolin at small discrete sites, while most of the nucleolin and nascent RNA was dispersed. In U87 cells, HS-anMan, nucleolin and nascent RNA reassembled after prolonged hypoxia.
Title: Nucleolin is a nuclear target of heparan sulfate derived from glypican-1.
A yeast display library based on an engineered IgG1 CH2 scaffold with diversified loop regions was constructed, and CH2 binders were isolated by panning against nucleolin.
Title: Engineered antibody CH2 domains binding to nucleolin: Isolation, characterization and improvement of aggregation.
NCL was associated with bipolar disorder.
Title: Whole-exome sequencing of individuals from an isolated population implicates rare risk variants in bipolar disorder.
The following NCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu17088 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_005381.2 | |
| Accession Version | NM_005381.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | COA |
| ORF Insert Method | CloneEZ® Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-06 | |
| Organism | Homo sapiens(human) | |
| Product | nucleolin | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM761058.1, AK001314.1, AK000221.1 and BQ001029.1. On or before Oct 3, 2009 this sequence version replaced XM_002342275.1, NM_005381.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.58922.1, SRR1163657.289658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length. | |
1 | ATGGTGAAGC TCGCGAAGGC AGGTAAAAAT CAAGGTGACC CCAAGAAAAT GGCTCCTCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_005372.2 |
| CDS | 142..2274 |
| Misc Feature(1) | 166..168 |
| Misc Feature(2) | 184..186 |
| Misc Feature(3) | 187..189 |
| Misc Feature(4) | 223..225 |
| Misc Feature(5) | 241..243 |
| Misc Feature(6) | 262..264 |
| Misc Feature(7) | 265..267 |
| Misc Feature(8) | 313..546 |
| Misc Feature(9) | 340..342 |
| Misc Feature(10) | 346..348 |
| Misc Feature(11) | 367..369 |
| Misc Feature(12) | 391..393 |
| Misc Feature(13) | 415..417 |
| Misc Feature(14) | 427..429 |
| Misc Feature(15) | 436..438 |
| Misc Feature(16) | 445..447 |
| Misc Feature(17) | 457..459 |
| Misc Feature(18) | 466..468 |
| Misc Feature(19) | 478..480 |
| Misc Feature(20) | 487..489 |
| Misc Feature(21) | 502..504 |
| Misc Feature(22) | 511..513 |
| Misc Feature(23) | 574..576 |
| Misc Feature(24) | 598..600 |
| Misc Feature(25) | 691..693 |
| Misc Feature(26) | 757..759 |
| Misc Feature(27) | 781..783 |
| Misc Feature(28) | 1042..1044 |
| Misc Feature(29) | 1060..1284 |
| Misc Feature(30) | order(1063..1065,1069..1071,1096..1101,1111..1113,1165..1170,1174..1182,1186..1188,1192..1194,1270..1272,1276..1278,1282..1284) |
| Misc Feature(31) | 1093..1095 |
| Misc Feature(32) | 1183..1185 |
| Misc Feature(33) | 1207..1209 |
| Misc Feature(34) | 1240..1242 |
| Misc Feature(35) | 1270..1272 |
| Misc Feature(36) | 1276..2025 |
| Misc Feature(37) | 1309..1536 |
| Misc Feature(38) | order(1309..1314,1327..1329,1333..1335,1399..1410,1426..1428,1432..1440,1525..1527,1531..1533) |
| Misc Feature(39) | 1333..1335 |
| Misc Feature(40) | 1348..1350 |
| Misc Feature(41) | 1354..1356 |
| Misc Feature(42) | 1420..1422 |
| Misc Feature(43) | 1471..1473 |
| Misc Feature(44) | 1513..1515 |
| Misc Feature(45) | 1519..1521 |
| Misc Feature(46) | 1540..1542 |
| Misc Feature(47) | 1570..1572 |
| Misc Feature(48) | 1594..1812 |
| Misc Feature(49) | 1678..1680 |
| Misc Feature(50) | 1702..1704 |
| Misc Feature(51) | 1828..1830 |
| Misc Feature(52) | 1855..2088 |
| Misc Feature(53) | 1855..1857 |
| Misc Feature(54) | 1870..1872 |
| Misc Feature(55) | 1879..1881 |
| Misc Feature(56) | 1912..1914 |
| Misc Feature(57) | 1996..1998 |
| Misc Feature(58) | 2077..2079 |
| Misc Feature(59) | 2107..2109 |
| Misc Feature(60) | 2119..2121 |
| Misc Feature(61) | 2137..2139 |
| Misc Feature(62) | 2149..2151 |
| Misc Feature(63) | 2158..2160 |
| Misc Feature(64) | 2221..2223 |
| Exon (1) | 1..159 |
| Exon (2) | 160..276 |
| Exon (3) | 277..754 |
| Exon (4) | 755..952 |
| Exon (5) | 953..1039 |
| Exon (6) | 1040..1181 |
| Exon (7) | 1182..1306 |
| Exon (8) | 1307..1430 |
| Exon (9) | 1431..1588 |
| Exon (10) | 1589..1712 |
| Exon (11) | 1713..1846 |
| Exon (12) | 1847..1973 |
| Exon (13) | 1974..2197 |
| Exon (14) | 2198..2717 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_005381.2 |
ORF Insert Sequence:
1 | ATGGTGAAGC TCGCGAAGGC AGGTAAAAAT CAAGGTGACC CCAAGAAAAT GGCTCCTCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Identification of Microprotein-Protein Interactions via APEX Tagging. |
|
Phorbol-12-myristate-13-acetate-mediated stabilization of leukemia inhibitory factor ( |
|
Nucleolin is a nuclear target of heparan sulfate derived from glypican-1. |
|
Engineered antibody CH2 domains binding to nucleolin: Isolation, characterization and improvement of aggregation. |
|
Whole-exome sequencing of individuals from an isolated population implicates rare risk variants in bipolar disorder. |