NIP7 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	NIP7
Entrez Gene ID	51388
Full Name	NIP7, nucleolar pre-rRNA processing protein
Synonyms	CGI-37,HSPC031,KD93
General protein information	Preferred Names NIP7, nucleolar pre-rRNA processing protein Names 60S ribosome subunit biogenesis protein NIP7 homolog nuclear import 7 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_016101.4	NP_057185.1	60S ribosome subunit biogenesis protein NIP7 homolog isoform 1
NM_016101.5	NP_057185.1	60S ribosome subunit biogenesis protein NIP7 homolog isoform 1
NM_001199434.1	NP_001186363.1	60S ribosome subunit biogenesis protein NIP7 homolog isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NIP7	NP_001179168.1
Mus musculus (house mouse)	Nip7	NP_079667.2
Neurospora crassa	NCU00105	XP_957229.2
Oryza sativa (rice)	Os07g0670600	NP_001060594.1
Rattus norvegicus (Norway rat)	Nip7	NP_620202.1
Danio rerio (zebrafish)	nip7	NP_001018426.1
Drosophila melanogaster (fruit fly)	CG7006	NP_651296.1
Arabidopsis thaliana (thale cress)	AT4G15770	NP_193312.5
	NIP7	XP_001099770.1
Caenorhabditis elegans (roundworm)	C43E11.9	NP_491342.1
Saccharomyces cerevisiae (baker's yeast)	NIP7	NP_015113.1
Kluyveromyces lactis	KLLA0B02266g	XP_451632.1
Magnaporthe oryzae (rice blast fungus)	MGG_00482	XP_003718580.1
Pan troglodytes (chimpanzee)	NIP7	NP_001233354.1
Gallus gallus (chicken)	NIP7	NP_001264418.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002330	XP_312639.4
Eremothecium gossypii	AGOS_AFL104W	NP_985446.1
Homo sapiens (human)	NIP7	NP_057185.1
Canis lupus familiaris (dog)	NIP7	XP_536804.1
Schizosaccharomyces pombe (fission yeast)	SPCC320.11c	NP_587718.1
Xenopus tropicalis (tropical clawed frog)	nip7	NP_001096512.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing.
Morello LG, Coltri PP, Quaresma AJ, Simabuco FM, Silva TC, Singh G, Nickerson JA, Oliveira CC, Moore MJ, Zanchin NI
PloS one6(12)e29174(2011)

The NIP7 protein is required for accurate pre-rRNA processing in human cells.
Morello LG, Hesling C, Coltri PP, Castilho BA, Rimokh R, Zanchin NI
Nucleic acids research39(2)648-65(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results presented in this work indicate a close functional interaction between NIP7 and FTSJ3 during pre-rRNA processing and show that FTSJ3 participates in ribosome synthesis in human cells.
Title: The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing.

Downregulation of NIP7 affects pre-rRNA processing, causing an imbalance of the 40S/60S subunit ratio.
Title: The NIP7 protein is required for accurate pre-rRNA processing in human cells.

SBDS is found in complexes containing the human Nip7 ortholog.
Title: The Shwachman-Bodian-Diamond syndrome associated protein interacts with HsNip7 and its down-regulation affects gene expression at the transcriptional and translational levels.

KD93 is a novel protein expressed in human hematopoietic stem/progenitor cells
Title: Crystal structure of KD93, a novel protein expressed in human hematopoietic stem/progenitor cells.

The following NIP7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIP7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32245	NM_016101.5 Latest version!	Homo sapiens nucleolar pre-rRNA processing protein NIP7 (NIP7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu06015	NM_001199434.1 Latest version!	Homo sapiens nucleolar pre-rRNA processing protein NIP7 (NIP7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu32245	NM_016101.4	Homo sapiens NIP7, nucleolar pre-rRNA processing protein (NIP7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIP7 ( NM_016101.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057185.1 Homo sapiens NIP7, nucleolar pre-rRNA processing protein (NIP7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu32245
Clone ID Related Accession (Same CDS sequence)	NM_016101.4 , NM_016101.5
Accession Version	NM_016101.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	60S ribosome subunit biogenesis protein NIP7 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB453898.1, BC015941.1, DR423435.1 and AW001672.1. On Dec 7, 2010 this sequence version replaced NM_016101.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.203409.1, SRR1803617.179643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCTT TGACTGAAGA GGAGACCCGT GTCATGTTTG AGAAGATAGC GAAATACATT 
GGGGAGAATC TTCAACTGCT GGTGGACCGG CCCGATGGCA CCTACTGTTT CCGTCTGCAC 
AACGACCGGG TGTACTATGT GAGTGAGAAG ATTATGAAGC TGGCCGCCAA TATTTCCGGG 
GACAAGCTGG TGTCGCTGGG GACCTGCTTT GGAAAATTCA CTAAAACCCA CAAGTTTCGG 
TTGCACGTCA CAGCTCTGGA TTACCTTGCA CCTTATGCCA AGTATAAAGT TTGGATAAAG 
CCTGGTGCAG AGCAGTCCTT CCTGTATGGG AACCATGTGT TGAAATCTGG TCTGGGTCGA 
ATCACTGAAA ATACTTCTCA GTACCAGGGC GTGGTGGTGT ACTCCATGGC AGACATCCCT 
TTGGGTTTTG GGGTGGCAGC CAAATCTACA CAAGACTGCA GAAAAGTAGA CCCCATGGCG 
ATTGTGGTAT TTCATCAAGC AGACATTGGG GAATATGTGC GGCATGAAGA GACGTTGACT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057185.1
CDS	319..861
Translation	MRPLTEEETRVMFEKIAKYIGENLQLLVDRPDGTYCFRLHNDRVYYVSEKIMKLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKYKVWIKPGAEQSFLYGNHVLKSGLGRITENTSQYQGVVVYSMADIPLGFGVAAKSTQDCRKVDPMAIVVFHQADIGEYVRHEETLT

Target ORF information:

RefSeq Version	NM_016101.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NIP7, nucleolar pre-rRNA processing protein (NIP7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016101.4

ORF Insert Sequence:

ATGCGGCCTT TGACTGAAGA GGAGACCCGT GTCATGTTTG AGAAGATAGC GAAATACATT 
GGGGAGAATC TTCAACTGCT GGTGGACCGG CCCGATGGCA CCTACTGTTT CCGTCTGCAC 
AACGACCGGG TGTACTATGT GAGTGAGAAG ATTATGAAGC TGGCCGCCAA TATTTCCGGG 
GACAAGCTGG TGTCGCTGGG GACCTGCTTT GGAAAATTCA CTAAAACCCA CAAGTTTCGG 
TTGCACGTCA CAGCTCTGGA TTACCTTGCA CCTTATGCCA AGTATAAAGT TTGGATAAAG 
CCTGGTGCAG AGCAGTCCTT CCTGTATGGG AACCATGTGT TGAAATCTGG TCTGGGTCGA 
ATCACTGAAA ATACTTCTCA GTACCAGGGC GTGGTGGTGT ACTCCATGGC AGACATCCCT 
TTGGGTTTTG GGGTGGCAGC CAAATCTACA CAAGACTGCA GAAAAGTAGA CCCCATGGCG 
ATTGTGGTAT TTCATCAAGC AGACATTGGG GAATATGTGC GGCATGAAGA GACGTTGACT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIP7 ( NM_016101.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057185.1 Homo sapiens nucleolar pre-rRNA processing protein NIP7 (NIP7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu32245
Clone ID Related Accession (Same CDS sequence)	NM_016101.4 , NM_016101.5
Accession Version	NM_016101.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	60S ribosome subunit biogenesis protein NIP7 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB453898.1, BC015941.1, DR423435.1 and AW001672.1. On Nov 22, 2018 this sequence version replaced NM_016101.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.126114.1, SRR1660809.153249.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000254940.10/ ENSP00000254940.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGCCTT TGACTGAAGA GGAGACCCGT GTCATGTTTG AGAAGATAGC GAAATACATT 
GGGGAGAATC TTCAACTGCT GGTGGACCGG CCCGATGGCA CCTACTGTTT CCGTCTGCAC 
AACGACCGGG TGTACTATGT GAGTGAGAAG ATTATGAAGC TGGCCGCCAA TATTTCCGGG 
GACAAGCTGG TGTCGCTGGG GACCTGCTTT GGAAAATTCA CTAAAACCCA CAAGTTTCGG 
TTGCACGTCA CAGCTCTGGA TTACCTTGCA CCTTATGCCA AGTATAAAGT TTGGATAAAG 
CCTGGTGCAG AGCAGTCCTT CCTGTATGGG AACCATGTGT TGAAATCTGG TCTGGGTCGA 
ATCACTGAAA ATACTTCTCA GTACCAGGGC GTGGTGGTGT ACTCCATGGC AGACATCCCT 
TTGGGTTTTG GGGTGGCAGC CAAATCTACA CAAGACTGCA GAAAAGTAGA CCCCATGGCG 
ATTGTGGTAT TTCATCAAGC AGACATTGGG GAATATGTGC GGCATGAAGA GACGTTGACT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057185.1
CDS	59..601
Translation	MRPLTEEETRVMFEKIAKYIGENLQLLVDRPDGTYCFRLHNDRVYYVSEKIMKLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKYKVWIKPGAEQSFLYGNHVLKSGLGRITENTSQYQGVVVYSMADIPLGFGVAAKSTQDCRKVDPMAIVVFHQADIGEYVRHEETLT

Target ORF information:

RefSeq Version	NM_016101.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar pre-rRNA processing protein NIP7 (NIP7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016101.5

ORF Insert Sequence:

ATGCGGCCTT TGACTGAAGA GGAGACCCGT GTCATGTTTG AGAAGATAGC GAAATACATT 
GGGGAGAATC TTCAACTGCT GGTGGACCGG CCCGATGGCA CCTACTGTTT CCGTCTGCAC 
AACGACCGGG TGTACTATGT GAGTGAGAAG ATTATGAAGC TGGCCGCCAA TATTTCCGGG 
GACAAGCTGG TGTCGCTGGG GACCTGCTTT GGAAAATTCA CTAAAACCCA CAAGTTTCGG 
TTGCACGTCA CAGCTCTGGA TTACCTTGCA CCTTATGCCA AGTATAAAGT TTGGATAAAG 
CCTGGTGCAG AGCAGTCCTT CCTGTATGGG AACCATGTGT TGAAATCTGG TCTGGGTCGA 
ATCACTGAAA ATACTTCTCA GTACCAGGGC GTGGTGGTGT ACTCCATGGC AGACATCCCT 
TTGGGTTTTG GGGTGGCAGC CAAATCTACA CAAGACTGCA GAAAAGTAGA CCCCATGGCG 
ATTGTGGTAT TTCATCAAGC AGACATTGGG GAATATGTGC GGCATGAAGA GACGTTGACT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIP7 ( NM_001199434.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186363.1 Homo sapiens nucleolar pre-rRNA processing protein NIP7 (NIP7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu06015
Clone ID Related Accession (Same CDS sequence)	NM_001199434.1
Accession Version	NM_001199434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Homo sapiens(human)
Product	60S ribosome subunit biogenesis protein NIP7 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB453898.1, AF161528.1, BC015941.1, DR423435.1 and AW001672.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF161528.1, SRR1163658.283.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCTT TGACTGAAGA GGAGACCCGT GTCATGTTTG AGAAGATAGC GAAATACATT 
GGGGAGAATC TTCAACTGCT GGTGGACCGG CCCGATGGCA CCTACTGTTT CCGTCTGCAC 
AACGACCGGG TGTACTATGT GAGTGAGAAG ATTATGAAGC TGGCCGCCAA TATTTCCGGG 
GACAAGCTGG TGTCGCTGGG GACCTGCTTT GGAAAATTCA CTAAAACCCA CAAGTTTCGG 
TTGCACGTCA CAGCTCTGGA TTACCTTGCA CCTTATGCCA AGGGTTTTGG GGTGGCAGCC 
AAATCTACAC AAGACTGCAG AAAAGTAGAC CCCATGGCGA TTGTGGTATT TCATCAAGCA 
GACATTGGGG AATATGTGCG GCATGAAGAG ACGTTGACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186363.1
CDS	319..720
Translation	MRPLTEEETRVMFEKIAKYIGENLQLLVDRPDGTYCFRLHNDRVYYVSEKIMKLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKGFGVAAKSTQDCRKVDPMAIVVFHQADIGEYVRHEETLT

Target ORF information:

RefSeq Version	NM_001199434.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar pre-rRNA processing protein NIP7 (NIP7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199434.1

ORF Insert Sequence:

ATGCGGCCTT TGACTGAAGA GGAGACCCGT GTCATGTTTG AGAAGATAGC GAAATACATT 
GGGGAGAATC TTCAACTGCT GGTGGACCGG CCCGATGGCA CCTACTGTTT CCGTCTGCAC 
AACGACCGGG TGTACTATGT GAGTGAGAAG ATTATGAAGC TGGCCGCCAA TATTTCCGGG 
GACAAGCTGG TGTCGCTGGG GACCTGCTTT GGAAAATTCA CTAAAACCCA CAAGTTTCGG 
TTGCACGTCA CAGCTCTGGA TTACCTTGCA CCTTATGCCA AGGGTTTTGG GGTGGCAGCC 
AAATCTACAC AAGACTGCAG AAAAGTAGAC CCCATGGCGA TTGTGGTATT TCATCAAGCA 
GACATTGGGG AATATGTGCG GCATGAAGAG ACGTTGACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing.
PloS one6(12)e29174(2011)
Morello LG,Coltri PP,Quaresma AJ,Simabuco FM,Silva TC,Singh G,Nickerson JA,Oliveira CC,Moore MJ,Zanchin NI

The NIP7 protein is required for accurate pre-rRNA processing in human cells.
Nucleic acids research39(2)648-65(2011 Jan)
Morello LG,Hesling C,Coltri PP,Castilho BA,Rimokh R,Zanchin NI

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NIP7 cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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