RAPGEF6 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001164387.1	NP_001157859.1	rap guanine nucleotide exchange factor 6 isoform 3
NM_001164389.1	NP_001157861.1	rap guanine nucleotide exchange factor 6 isoform 5
NM_016340.5	NP_057424.3	rap guanine nucleotide exchange factor 6 isoform 2
NM_001164386.1	NP_001157858.1	rap guanine nucleotide exchange factor 6 isoform 1
NM_001164390.1	NP_001157862.1	rap guanine nucleotide exchange factor 6 isoform 6
NM_001164390.2	NP_001157862.1	rap guanine nucleotide exchange factor 6 isoform 6
NM_016340.6	NP_057424.3	rap guanine nucleotide exchange factor 6 isoform 2
NM_001164387.2	NP_001157859.1	rap guanine nucleotide exchange factor 6 isoform 3
NM_001164388.1	NP_001157860.1	rap guanine nucleotide exchange factor 6 isoform 4
NM_001164389.2	NP_001157861.1	rap guanine nucleotide exchange factor 6 isoform 5
NM_001164388.2	NP_001157860.1	rap guanine nucleotide exchange factor 6 isoform 4
NM_001164386.2	NP_001157858.1	rap guanine nucleotide exchange factor 6 isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03513	NM_001164386.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu12289	NM_001164390.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu02298	NM_016340.6 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu03516	NM_001164387.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu03517	NM_001164388.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu03520	NM_001164389.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
View Old Accession Versions >>
OHu12289	NM_001164390.1	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu02298	NM_016340.5	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu03516	NM_001164387.1	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu03520	NM_001164389.1	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu03517	NM_001164388.1	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu03513	NM_001164386.1	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
Hide Old Accession Versions >>

RAPGEF6 ( NM_001164386.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157858.1 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03513
Clone ID Related Accession (Same CDS sequence)	NM_001164386.1 , NM_001164386.2
Accession Version	NM_001164386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, BC140820.1, AV726501.2, AL832899.1 and DA817493.1. On Jun 2, 2019 this sequence version replaced NM_001164386.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.278521.1, SRR1660805.16921.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGA 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGC 
AGCTCTCTGC TTAATGCCAA GAAGCTATAT GAGGATGCCC AAATGGCAAG GAAGGTGAAG 
CAGTATCTTT CCAGTCTCGA TGTAGAGACA GATGAGGAGA AGTTCCAGAT GATGTCATTA 
CAGTGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA GTGAGAAAAG ATCAGCCAAA 
TCATCTGAAA TGTCTCCAGT GCCTATGAGG TCAGCTGGCC AAACAACTAA AGCCCACTTG 
CATCAACCCC ACAGAGTAAG CCAGGTGCTT CAGGTGCCAG CTGTTAATTT GCACCCCATC 
AGGAAGAAGG GACAAACAAA AGACCCTGCA CTGAATACAA GTTTACCTCA GAAAGTTTTA 
GGAACAACTG AAGAAATAAG TGGTAAGAAG CATACAGAAG ACACTATTTC TGTGGCGTCA 
TCTTTACATT CTAGTCCTCC TGCATCTCCT CAAGGCTCCC CTCACAAAGG TTACACACTT 
ATTCCATCAG CTAAATCTGA CAACTTGTCT GACTCCAGCC ATAGTGAGAT TTCTTCACGG 
TCCAGCATCG TGAGCAATTG TTCTGTTGAC TCCATGTCTG CAGCTCTACA GGATGAACGG 
TGTTCCTCTC AGGCCCTGGC AGTCCCTGAA TCCACTGGGG CATTGGAAAA GACAGAGCAC 
GCTTCAGGGA TAGGAGATCA TAGTCAACAT GGCCCTGGGT GGACACTCTT GAAGCCATCT 
CTAATCAAGT GTTTAGCTGT CTCATCGTCT GTGAGCAATG AAGAGATTTC TCAAGAGCAT 
ATCATTATAG AAGCAGCTGA CAGTGGTCGT GGAAGTTGGA CTTCGTGTTC AAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCCAAACCCA AAAAGCTGGG ATTTTTTGAA CTCTTACAGA 
CATACCCATT TGGATGACCC CATTGCTGAA GTTGAACCCA CTGACTCTGA GCCCTATTCC 
TGTTCTAAAA GCTGCTCTAG AACTTGTGGG CAGTGTAAAG GAAGCCTAGA GAGAAAGAGT 
TGGACCTCCT CCAGTTCTCT GTCTGACACG TATGAACCAA ACTATGGGAC AGTTAAACAG 
AGAGTATTGG AGAGCACCCC AGCTGAGTCA TCTGAAGGCT TGGACCCCAA GGATGCCACT 
GACCCAGTTT ATAAAACTGT CACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGACGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATATTTG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACACAC ACCTAAAACC TCCAGATTAT 
AGTGTGGCAG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAACCTCGT GGCCTGTGTT CCATCGAAGA TTGTAACTCA GCCTCAGAGG 
CATAATTTGC AGCCATTCCA TCCTAAACTA GGAGATGTGA CTGATGCAGA TAGCGAAGCA 
GATGAAAATG AACAAGTTTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157858.1
CDS	200..5029
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKDATDPVYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYLGISLADLKEGPHTHLKPPDYSVAVQRSKMMHNSLSRLPPASLSSNLVACVPSKIVTQPQRHNLQPFHPKLGDVTDADSEADENEQVSAV

Target ORF information:

RefSeq Version	NM_001164386.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164386.2

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGA 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGC 
AGCTCTCTGC TTAATGCCAA GAAGCTATAT GAGGATGCCC AAATGGCAAG GAAGGTGAAG 
CAGTATCTTT CCAGTCTCGA TGTAGAGACA GATGAGGAGA AGTTCCAGAT GATGTCATTA 
CAGTGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA GTGAGAAAAG ATCAGCCAAA 
TCATCTGAAA TGTCTCCAGT GCCTATGAGG TCAGCTGGCC AAACAACTAA AGCCCACTTG 
CATCAACCCC ACAGAGTAAG CCAGGTGCTT CAGGTGCCAG CTGTTAATTT GCACCCCATC 
AGGAAGAAGG GACAAACAAA AGACCCTGCA CTGAATACAA GTTTACCTCA GAAAGTTTTA 
GGAACAACTG AAGAAATAAG TGGTAAGAAG CATACAGAAG ACACTATTTC TGTGGCGTCA 
TCTTTACATT CTAGTCCTCC TGCATCTCCT CAAGGCTCCC CTCACAAAGG TTACACACTT 
ATTCCATCAG CTAAATCTGA CAACTTGTCT GACTCCAGCC ATAGTGAGAT TTCTTCACGG 
TCCAGCATCG TGAGCAATTG TTCTGTTGAC TCCATGTCTG CAGCTCTACA GGATGAACGG 
TGTTCCTCTC AGGCCCTGGC AGTCCCTGAA TCCACTGGGG CATTGGAAAA GACAGAGCAC 
GCTTCAGGGA TAGGAGATCA TAGTCAACAT GGCCCTGGGT GGACACTCTT GAAGCCATCT 
CTAATCAAGT GTTTAGCTGT CTCATCGTCT GTGAGCAATG AAGAGATTTC TCAAGAGCAT 
ATCATTATAG AAGCAGCTGA CAGTGGTCGT GGAAGTTGGA CTTCGTGTTC AAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCCAAACCCA AAAAGCTGGG ATTTTTTGAA CTCTTACAGA 
CATACCCATT TGGATGACCC CATTGCTGAA GTTGAACCCA CTGACTCTGA GCCCTATTCC 
TGTTCTAAAA GCTGCTCTAG AACTTGTGGG CAGTGTAAAG GAAGCCTAGA GAGAAAGAGT 
TGGACCTCCT CCAGTTCTCT GTCTGACACG TATGAACCAA ACTATGGGAC AGTTAAACAG 
AGAGTATTGG AGAGCACCCC AGCTGAGTCA TCTGAAGGCT TGGACCCCAA GGATGCCACT 
GACCCAGTTT ATAAAACTGT CACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGACGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATATTTG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACACAC ACCTAAAACC TCCAGATTAT 
AGTGTGGCAG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAACCTCGT GGCCTGTGTT CCATCGAAGA TTGTAACTCA GCCTCAGAGG 
CATAATTTGC AGCCATTCCA TCCTAAACTA GGAGATGTGA CTGATGCAGA TAGCGAAGCA 
GATGAAAATG AACAAGTTTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_001164390.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157862.1 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu12289
Clone ID Related Accession (Same CDS sequence)	NM_001164390.1 , NM_001164390.2
Accession Version	NM_001164390.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, BC142964.1 and AC004622.1. Transcript Variant: This variant (6) differs in the 3' UTR and lacks a significant portion of the 3' coding region, compared to variant 1. This results in a protein (isoform 6) that maintains the reading frame but is shorter at the C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC142964.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157862.1
CDS	207..2690
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGR

Target ORF information:

RefSeq Version	NM_001164390.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164390.1

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_001164390.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157862.1 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 6, mRNA.

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CloneID	OHu12289
Clone ID Related Accession (Same CDS sequence)	NM_001164390.1 , NM_001164390.2
Accession Version	NM_001164390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, BC142964.1 and AC004622.1. On Jun 2, 2019 this sequence version replaced NM_001164390.1. Transcript Variant: This variant (6) differs in the 3' UTR and lacks a significant portion of the 3' coding region, compared to variant 1. This results in a protein (isoform 6) that maintains the reading frame but is shorter at the C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC142964.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157862.1
CDS	200..2683
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGR

Target ORF information:

RefSeq Version	NM_001164390.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164390.2

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_016340.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057424.3 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02298
Clone ID Related Accession (Same CDS sequence)	NM_016340.5 , NM_016340.6
Accession Version	NM_016340.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, BC144627.1, DA817493.1 and AL832899.1. On Aug 26, 2009 this sequence version replaced NM_016340.4. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF478567.1, AF478468.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGAAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGCAGCT CTCTGCTTAA TGCCAAGAAG 
CTATATGAGG ATGCCCAAAT GGCAAGGAAG GTGAAGCAGT ATCTTTCCAG TCTCGATGTA 
GAGACAGATG AGGAGAAGTT CCAGATGATG TCATTACAGT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CTGAAATGTC TCCAGTGCCT 
ATGAGGTCAG CTGGCCAAAC AACTAAAGCC CACTTGCATC AACCCCACAG AGTAAGCCAG 
GTGCTTCAGG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AACAAAAGAC 
CCTGCACTGA ATACAAGTTT ACCTCAGAAA GTTTTAGGAA CAACTGAAGA AATAAGTGGT 
AAGAAGCATA CAGAAGACAC TATTTCTGTG GCGTCATCTT TACATTCTAG TCCTCCTGCA 
TCTCCTCAAG GCTCCCCTCA CAAAGGTTAC ACACTTATTC CATCAGCTAA ATCTGACAAC 
TTGTCTGACT CCAGCCATAG TGAGATTTCT TCACGGTCCA GCATCGTGAG CAATTGTTCT 
GTTGACTCCA TGTCTGCAGC TCTACAGGAT GAACGGTGTT CCTCTCAGGC CCTGGCAGTC 
CCTGAATCCA CTGGGGCATT GGAAAAGACA GAGCACGCTT CAGGGATAGG AGATCATAGT 
CAACATGGCC CTGGGTGGAC ACTCTTGAAG CCATCTCTAA TCAAGTGTTT AGCTGTCTCA 
TCGTCTGTGA GCAATGAAGA GATTTCTCAA GAGCATATCA TTATAGAAGC AGCTGACAGT 
GGTCGTGGAA GTTGGACTTC GTGTTCAAGC AGCTCCCATG ACAACTTCCA AAGCCTTCCA 
AACCCAAAAA GCTGGGATTT TTTGAACTCT TACAGACATA CCCATTTGGA TGACCCCATT 
GCTGAAGTTG AACCCACTGA CTCTGAGCCC TATTCCTGTT CTAAAAGCTG CTCTAGAACT 
TGTGGGCAGT GTAAAGGAAG CCTAGAGAGA AAGAGTTGGA CCTCCTCCAG TTCTCTGTCT 
GACACGTATG AACCAAACTA TGGGACAGTT AAACAGAGAG TATTGGAGAG CACCCCAGCT 
GAGTCATCTG AAGGCTTGGA CCCCAAGGAT GCCACTGACC CAGTTTATAA AACTGTCACT 
TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGACGATAGG 
TATAGGGAGC CACCTCCCAC TCCTCCAGGA TATTTGGGGA TTTCTTTAGC GGACCTAAAG 
GAAGGACCCC ACACACACCT AAAACCTCCA GATTATAGTG TGGCAGTGCA GAGGTCAAAG 
ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAA CCTCGTGGCC 
TGTGTTCCAT CGAAGATTGT AACTCAGCCT CAGAGGCATA ATTTGCAGCC ATTCCATCCT 
AAACTAGGAG ATGTGACTGA TGCAGATAGC GAAGCAGATG AAAATGAACA AGTTTCAGCA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057424.3
CDS	207..5012
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKDATDPVYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYLGISLADLKEGPHTHLKPPDYSVAVQRSKMMHNSLSRLPPASLSSNLVACVPSKIVTQPQRHNLQPFHPKLGDVTDADSEADENEQVSAV

Target ORF information:

RefSeq Version	NM_016340.5
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016340.5

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGAAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGCAGCT CTCTGCTTAA TGCCAAGAAG 
CTATATGAGG ATGCCCAAAT GGCAAGGAAG GTGAAGCAGT ATCTTTCCAG TCTCGATGTA 
GAGACAGATG AGGAGAAGTT CCAGATGATG TCATTACAGT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CTGAAATGTC TCCAGTGCCT 
ATGAGGTCAG CTGGCCAAAC AACTAAAGCC CACTTGCATC AACCCCACAG AGTAAGCCAG 
GTGCTTCAGG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AACAAAAGAC 
CCTGCACTGA ATACAAGTTT ACCTCAGAAA GTTTTAGGAA CAACTGAAGA AATAAGTGGT 
AAGAAGCATA CAGAAGACAC TATTTCTGTG GCGTCATCTT TACATTCTAG TCCTCCTGCA 
TCTCCTCAAG GCTCCCCTCA CAAAGGTTAC ACACTTATTC CATCAGCTAA ATCTGACAAC 
TTGTCTGACT CCAGCCATAG TGAGATTTCT TCACGGTCCA GCATCGTGAG CAATTGTTCT 
GTTGACTCCA TGTCTGCAGC TCTACAGGAT GAACGGTGTT CCTCTCAGGC CCTGGCAGTC 
CCTGAATCCA CTGGGGCATT GGAAAAGACA GAGCACGCTT CAGGGATAGG AGATCATAGT 
CAACATGGCC CTGGGTGGAC ACTCTTGAAG CCATCTCTAA TCAAGTGTTT AGCTGTCTCA 
TCGTCTGTGA GCAATGAAGA GATTTCTCAA GAGCATATCA TTATAGAAGC AGCTGACAGT 
GGTCGTGGAA GTTGGACTTC GTGTTCAAGC AGCTCCCATG ACAACTTCCA AAGCCTTCCA 
AACCCAAAAA GCTGGGATTT TTTGAACTCT TACAGACATA CCCATTTGGA TGACCCCATT 
GCTGAAGTTG AACCCACTGA CTCTGAGCCC TATTCCTGTT CTAAAAGCTG CTCTAGAACT 
TGTGGGCAGT GTAAAGGAAG CCTAGAGAGA AAGAGTTGGA CCTCCTCCAG TTCTCTGTCT 
GACACGTATG AACCAAACTA TGGGACAGTT AAACAGAGAG TATTGGAGAG CACCCCAGCT 
GAGTCATCTG AAGGCTTGGA CCCCAAGGAT GCCACTGACC CAGTTTATAA AACTGTCACT 
TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGACGATAGG 
TATAGGGAGC CACCTCCCAC TCCTCCAGGA TATTTGGGGA TTTCTTTAGC GGACCTAAAG 
GAAGGACCCC ACACACACCT AAAACCTCCA GATTATAGTG TGGCAGTGCA GAGGTCAAAG 
ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAA CCTCGTGGCC 
TGTGTTCCAT CGAAGATTGT AACTCAGCCT CAGAGGCATA ATTTGCAGCC ATTCCATCCT 
AAACTAGGAG ATGTGACTGA TGCAGATAGC GAAGCAGATG AAAATGAACA AGTTTCAGCA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_016340.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_057424.3 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 2, mRNA.

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CloneID	OHu02298
Clone ID Related Accession (Same CDS sequence)	NM_016340.5 , NM_016340.6
Accession Version	NM_016340.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, BC144627.1, DA817493.1 and AL832899.1. On Jun 2, 2019 this sequence version replaced NM_016340.5. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF478567.1, AF478468.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGAAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGCAGCT CTCTGCTTAA TGCCAAGAAG 
CTATATGAGG ATGCCCAAAT GGCAAGGAAG GTGAAGCAGT ATCTTTCCAG TCTCGATGTA 
GAGACAGATG AGGAGAAGTT CCAGATGATG TCATTACAGT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CTGAAATGTC TCCAGTGCCT 
ATGAGGTCAG CTGGCCAAAC AACTAAAGCC CACTTGCATC AACCCCACAG AGTAAGCCAG 
GTGCTTCAGG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AACAAAAGAC 
CCTGCACTGA ATACAAGTTT ACCTCAGAAA GTTTTAGGAA CAACTGAAGA AATAAGTGGT 
AAGAAGCATA CAGAAGACAC TATTTCTGTG GCGTCATCTT TACATTCTAG TCCTCCTGCA 
TCTCCTCAAG GCTCCCCTCA CAAAGGTTAC ACACTTATTC CATCAGCTAA ATCTGACAAC 
TTGTCTGACT CCAGCCATAG TGAGATTTCT TCACGGTCCA GCATCGTGAG CAATTGTTCT 
GTTGACTCCA TGTCTGCAGC TCTACAGGAT GAACGGTGTT CCTCTCAGGC CCTGGCAGTC 
CCTGAATCCA CTGGGGCATT GGAAAAGACA GAGCACGCTT CAGGGATAGG AGATCATAGT 
CAACATGGCC CTGGGTGGAC ACTCTTGAAG CCATCTCTAA TCAAGTGTTT AGCTGTCTCA 
TCGTCTGTGA GCAATGAAGA GATTTCTCAA GAGCATATCA TTATAGAAGC AGCTGACAGT 
GGTCGTGGAA GTTGGACTTC GTGTTCAAGC AGCTCCCATG ACAACTTCCA AAGCCTTCCA 
AACCCAAAAA GCTGGGATTT TTTGAACTCT TACAGACATA CCCATTTGGA TGACCCCATT 
GCTGAAGTTG AACCCACTGA CTCTGAGCCC TATTCCTGTT CTAAAAGCTG CTCTAGAACT 
TGTGGGCAGT GTAAAGGAAG CCTAGAGAGA AAGAGTTGGA CCTCCTCCAG TTCTCTGTCT 
GACACGTATG AACCAAACTA TGGGACAGTT AAACAGAGAG TATTGGAGAG CACCCCAGCT 
GAGTCATCTG AAGGCTTGGA CCCCAAGGAT GCCACTGACC CAGTTTATAA AACTGTCACT 
TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGACGATAGG 
TATAGGGAGC CACCTCCCAC TCCTCCAGGA TATTTGGGGA TTTCTTTAGC GGACCTAAAG 
GAAGGACCCC ACACACACCT AAAACCTCCA GATTATAGTG TGGCAGTGCA GAGGTCAAAG 
ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAA CCTCGTGGCC 
TGTGTTCCAT CGAAGATTGT AACTCAGCCT CAGAGGCATA ATTTGCAGCC ATTCCATCCT 
AAACTAGGAG ATGTGACTGA TGCAGATAGC GAAGCAGATG AAAATGAACA AGTTTCAGCA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057424.3
CDS	200..5005
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKDATDPVYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYLGISLADLKEGPHTHLKPPDYSVAVQRSKMMHNSLSRLPPASLSSNLVACVPSKIVTQPQRHNLQPFHPKLGDVTDADSEADENEQVSAV

Target ORF information:

RefSeq Version	NM_016340.6
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016340.6

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGAAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGCAGCT CTCTGCTTAA TGCCAAGAAG 
CTATATGAGG ATGCCCAAAT GGCAAGGAAG GTGAAGCAGT ATCTTTCCAG TCTCGATGTA 
GAGACAGATG AGGAGAAGTT CCAGATGATG TCATTACAGT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CTGAAATGTC TCCAGTGCCT 
ATGAGGTCAG CTGGCCAAAC AACTAAAGCC CACTTGCATC AACCCCACAG AGTAAGCCAG 
GTGCTTCAGG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AACAAAAGAC 
CCTGCACTGA ATACAAGTTT ACCTCAGAAA GTTTTAGGAA CAACTGAAGA AATAAGTGGT 
AAGAAGCATA CAGAAGACAC TATTTCTGTG GCGTCATCTT TACATTCTAG TCCTCCTGCA 
TCTCCTCAAG GCTCCCCTCA CAAAGGTTAC ACACTTATTC CATCAGCTAA ATCTGACAAC 
TTGTCTGACT CCAGCCATAG TGAGATTTCT TCACGGTCCA GCATCGTGAG CAATTGTTCT 
GTTGACTCCA TGTCTGCAGC TCTACAGGAT GAACGGTGTT CCTCTCAGGC CCTGGCAGTC 
CCTGAATCCA CTGGGGCATT GGAAAAGACA GAGCACGCTT CAGGGATAGG AGATCATAGT 
CAACATGGCC CTGGGTGGAC ACTCTTGAAG CCATCTCTAA TCAAGTGTTT AGCTGTCTCA 
TCGTCTGTGA GCAATGAAGA GATTTCTCAA GAGCATATCA TTATAGAAGC AGCTGACAGT 
GGTCGTGGAA GTTGGACTTC GTGTTCAAGC AGCTCCCATG ACAACTTCCA AAGCCTTCCA 
AACCCAAAAA GCTGGGATTT TTTGAACTCT TACAGACATA CCCATTTGGA TGACCCCATT 
GCTGAAGTTG AACCCACTGA CTCTGAGCCC TATTCCTGTT CTAAAAGCTG CTCTAGAACT 
TGTGGGCAGT GTAAAGGAAG CCTAGAGAGA AAGAGTTGGA CCTCCTCCAG TTCTCTGTCT 
GACACGTATG AACCAAACTA TGGGACAGTT AAACAGAGAG TATTGGAGAG CACCCCAGCT 
GAGTCATCTG AAGGCTTGGA CCCCAAGGAT GCCACTGACC CAGTTTATAA AACTGTCACT 
TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGACGATAGG 
TATAGGGAGC CACCTCCCAC TCCTCCAGGA TATTTGGGGA TTTCTTTAGC GGACCTAAAG 
GAAGGACCCC ACACACACCT AAAACCTCCA GATTATAGTG TGGCAGTGCA GAGGTCAAAG 
ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAA CCTCGTGGCC 
TGTGTTCCAT CGAAGATTGT AACTCAGCCT CAGAGGCATA ATTTGCAGCC ATTCCATCCT 
AAACTAGGAG ATGTGACTGA TGCAGATAGC GAAGCAGATG AAAATGAACA AGTTTCAGCA 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_001164386.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157858.1 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 1, mRNA.

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CloneID	OHu03513
Clone ID Related Accession (Same CDS sequence)	NM_001164386.1 , NM_001164386.2
Accession Version	NM_001164386.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, BC140820.1, AV726501.2, AL832899.1 and DA817493.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.278521.1, SRR1660805.16921.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGA 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGC 
AGCTCTCTGC TTAATGCCAA GAAGCTATAT GAGGATGCCC AAATGGCAAG GAAGGTGAAG 
CAGTATCTTT CCAGTCTCGA TGTAGAGACA GATGAGGAGA AGTTCCAGAT GATGTCATTA 
CAGTGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA GTGAGAAAAG ATCAGCCAAA 
TCATCTGAAA TGTCTCCAGT GCCTATGAGG TCAGCTGGCC AAACAACTAA AGCCCACTTG 
CATCAACCCC ACAGAGTAAG CCAGGTGCTT CAGGTGCCAG CTGTTAATTT GCACCCCATC 
AGGAAGAAGG GACAAACAAA AGACCCTGCA CTGAATACAA GTTTACCTCA GAAAGTTTTA 
GGAACAACTG AAGAAATAAG TGGTAAGAAG CATACAGAAG ACACTATTTC TGTGGCGTCA 
TCTTTACATT CTAGTCCTCC TGCATCTCCT CAAGGCTCCC CTCACAAAGG TTACACACTT 
ATTCCATCAG CTAAATCTGA CAACTTGTCT GACTCCAGCC ATAGTGAGAT TTCTTCACGG 
TCCAGCATCG TGAGCAATTG TTCTGTTGAC TCCATGTCTG CAGCTCTACA GGATGAACGG 
TGTTCCTCTC AGGCCCTGGC AGTCCCTGAA TCCACTGGGG CATTGGAAAA GACAGAGCAC 
GCTTCAGGGA TAGGAGATCA TAGTCAACAT GGCCCTGGGT GGACACTCTT GAAGCCATCT 
CTAATCAAGT GTTTAGCTGT CTCATCGTCT GTGAGCAATG AAGAGATTTC TCAAGAGCAT 
ATCATTATAG AAGCAGCTGA CAGTGGTCGT GGAAGTTGGA CTTCGTGTTC AAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCCAAACCCA AAAAGCTGGG ATTTTTTGAA CTCTTACAGA 
CATACCCATT TGGATGACCC CATTGCTGAA GTTGAACCCA CTGACTCTGA GCCCTATTCC 
TGTTCTAAAA GCTGCTCTAG AACTTGTGGG CAGTGTAAAG GAAGCCTAGA GAGAAAGAGT 
TGGACCTCCT CCAGTTCTCT GTCTGACACG TATGAACCAA ACTATGGGAC AGTTAAACAG 
AGAGTATTGG AGAGCACCCC AGCTGAGTCA TCTGAAGGCT TGGACCCCAA GGATGCCACT 
GACCCAGTTT ATAAAACTGT CACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGACGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATATTTG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACACAC ACCTAAAACC TCCAGATTAT 
AGTGTGGCAG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAACCTCGT GGCCTGTGTT CCATCGAAGA TTGTAACTCA GCCTCAGAGG 
CATAATTTGC AGCCATTCCA TCCTAAACTA GGAGATGTGA CTGATGCAGA TAGCGAAGCA 
GATGAAAATG AACAAGTTTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157858.1
CDS	207..5036
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKDATDPVYKTVTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYLGISLADLKEGPHTHLKPPDYSVAVQRSKMMHNSLSRLPPASLSSNLVACVPSKIVTQPQRHNLQPFHPKLGDVTDADSEADENEQVSAV

Target ORF information:

RefSeq Version	NM_001164386.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164386.1

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGA 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGC 
AGCTCTCTGC TTAATGCCAA GAAGCTATAT GAGGATGCCC AAATGGCAAG GAAGGTGAAG 
CAGTATCTTT CCAGTCTCGA TGTAGAGACA GATGAGGAGA AGTTCCAGAT GATGTCATTA 
CAGTGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA GTGAGAAAAG ATCAGCCAAA 
TCATCTGAAA TGTCTCCAGT GCCTATGAGG TCAGCTGGCC AAACAACTAA AGCCCACTTG 
CATCAACCCC ACAGAGTAAG CCAGGTGCTT CAGGTGCCAG CTGTTAATTT GCACCCCATC 
AGGAAGAAGG GACAAACAAA AGACCCTGCA CTGAATACAA GTTTACCTCA GAAAGTTTTA 
GGAACAACTG AAGAAATAAG TGGTAAGAAG CATACAGAAG ACACTATTTC TGTGGCGTCA 
TCTTTACATT CTAGTCCTCC TGCATCTCCT CAAGGCTCCC CTCACAAAGG TTACACACTT 
ATTCCATCAG CTAAATCTGA CAACTTGTCT GACTCCAGCC ATAGTGAGAT TTCTTCACGG 
TCCAGCATCG TGAGCAATTG TTCTGTTGAC TCCATGTCTG CAGCTCTACA GGATGAACGG 
TGTTCCTCTC AGGCCCTGGC AGTCCCTGAA TCCACTGGGG CATTGGAAAA GACAGAGCAC 
GCTTCAGGGA TAGGAGATCA TAGTCAACAT GGCCCTGGGT GGACACTCTT GAAGCCATCT 
CTAATCAAGT GTTTAGCTGT CTCATCGTCT GTGAGCAATG AAGAGATTTC TCAAGAGCAT 
ATCATTATAG AAGCAGCTGA CAGTGGTCGT GGAAGTTGGA CTTCGTGTTC AAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCCAAACCCA AAAAGCTGGG ATTTTTTGAA CTCTTACAGA 
CATACCCATT TGGATGACCC CATTGCTGAA GTTGAACCCA CTGACTCTGA GCCCTATTCC 
TGTTCTAAAA GCTGCTCTAG AACTTGTGGG CAGTGTAAAG GAAGCCTAGA GAGAAAGAGT 
TGGACCTCCT CCAGTTCTCT GTCTGACACG TATGAACCAA ACTATGGGAC AGTTAAACAG 
AGAGTATTGG AGAGCACCCC AGCTGAGTCA TCTGAAGGCT TGGACCCCAA GGATGCCACT 
GACCCAGTTT ATAAAACTGT CACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGACGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATATTTG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACACAC ACCTAAAACC TCCAGATTAT 
AGTGTGGCAG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAACCTCGT GGCCTGTGTT CCATCGAAGA TTGTAACTCA GCCTCAGAGG 
CATAATTTGC AGCCATTCCA TCCTAAACTA GGAGATGTGA CTGATGCAGA TAGCGAAGCA 
GATGAAAATG AACAAGTTTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_001164387.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157859.1 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 3, mRNA.

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CloneID	OHu03516
Clone ID Related Accession (Same CDS sequence)	NM_001164387.1 , NM_001164387.2
Accession Version	NM_001164387.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, AF394782.1, AV726501.2, BU568060.1, DA817493.1 and AL832899.1. Transcript Variant: This variant (3) includes an alternate in-frame exon in the central coding region but lacks another alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.52321.1, AF394782.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGC TGTTGGTTTT TATTATATCC CTGATCAAGT TATAAGAGTT 
TTCAAAGTGG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACCAC AGCTAAAGAA 
GTAGTTTTTC ATGCTGTTCA TGAATTTGGT TTGACCGGTG CATCCGACAC ATATTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTC ATAAAACAGA GAAGACTTCC AGATCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACTTAAAAAA TAACATGGAA 
ACAGAAACCT TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTATCC 
ATGCTGCAGC TCAGTACCAT TGAGGTGGCC ACCCAGCTGT CAATGAGGGA CTTTGATTTG 
TTTCGTAATA TTGAACCGAC TGAGTACATC GATGACCTTT TTAAGTTAAA TTCCAAAACA 
GGAAATACTC ATTTGAAGAG GTTTGAGGAC ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAAATTT TAACTGAAGC AAATCAGCTC AAACGAATGA AGATTATTAA GCATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCATCTGT AGCAAGACTC AGAGGAACTT GGGAAAAGTT ACCAAGCAAA 
TACGAGAAAC ATCTTCAAGA TCTACAAGAC ATTTTTGATC CATCTAGAAA CATGGCAAAG 
TATAGAAATA TTCTTAGTAG TCAAAGTATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGACT CCAAAGTAGA TGGTTTAGTA 
AACTTTGAGA AGTTAAGAAT GATTTCCAAG GAAATCCGCC AAGTTGTTCG AATGACTTCT 
GCTAACATGG ACCCAGCTAT GATGTTTCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCACTGAGTC AAGGAAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA 
AAAAGGGCAC GCCGCAGCTC TCTGCTTAAT GCCAAGAAGC TATATGAGGA TGCCCAAATG 
GCAAGGAAGG TGAAGCAGTA TCTTTCCAGT CTCGATGTAG AGACAGATGA GGAGAAGTTC 
CAGATGATGT CATTACAGTG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAGTGAG 
AAAAGATCAG CCAAATCATC TGAAATGTCT CCAGTGCCTA TGAGGTCAGC TGGCCAAACA 
ACTAAAGCCC ACTTGCATCA ACCCCACAGA GTAAGCCAGG TGCTTCAGGT GCCAGCTGTT 
AATTTGCACC CCATCAGGAA GAAGGGACAA ACAAAAGACC CTGCACTGAA TACAAGTTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA ATAAGTGGTA AGAAGCATAC AGAAGACACT 
ATTTCTGTGG CGTCATCTTT ACATTCTAGT CCTCCTGCAT CTCCTCAAGG CTCCCCTCAC 
AAAGGTTACA CACTTATTCC ATCAGCTAAA TCTGACAACT TGTCTGACTC CAGCCATAGT 
GAGATTTCTT CACGGTCCAG CATCGTGAGC AATTGTTCTG TTGACTCCAT GTCTGCAGCT 
CTACAGGATG AACGGTGTTC CTCTCAGGCC CTGGCAGTCC CTGAATCCAC TGGGGCATTG 
GAAAAGACAG AGCACGCTTC AGGGATAGGA GATCATAGTC AACATGGCCC TGGGTGGACA 
CTCTTGAAGC CATCTCTAAT CAAGTGTTTA GCTGTCTCAT CGTCTGTGAG CAATGAAGAG 
ATTTCTCAAG AGCATATCAT TATAGAAGCA GCTGACAGTG GTCGTGGAAG TTGGACTTCG 
TGTTCAAGCA GCTCCCATGA CAACTTCCAA AGCCTTCCAA ACCCAAAAAG CTGGGATTTT 
TTGAACTCTT ACAGACATAC CCATTTGGAT GACCCCATTG CTGAAGTTGA ACCCACTGAC 
TCTGAGCCCT ATTCCTGTTC TAAAAGCTGC TCTAGAACTT GTGGGCAGTG TAAAGGAAGC 
CTAGAGAGAA AGAGTTGGAC CTCCTCCAGT TCTCTGTCTG ACACGTATGA ACCAAACTAT 
GGGACAGTTA AACAGAGAGT ATTGGAGAGC ACCCCAGCTG AGTCATCTGA AGGCTTGGAC 
CCCAAGGATG CCACTGACCC AGTTTATAAA ACTGTCACTT CAAGTACAGA AAAGGGCTTG 
ATTGAAAATG AACAAGTTTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157859.1
CDS	207..4736
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKDATDPVYKTVTSSTEKGLIENEQVSAV

Target ORF information:

RefSeq Version	NM_001164387.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164387.1

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGC TGTTGGTTTT TATTATATCC CTGATCAAGT TATAAGAGTT 
TTCAAAGTGG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACCAC AGCTAAAGAA 
GTAGTTTTTC ATGCTGTTCA TGAATTTGGT TTGACCGGTG CATCCGACAC ATATTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTC ATAAAACAGA GAAGACTTCC AGATCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACTTAAAAAA TAACATGGAA 
ACAGAAACCT TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTATCC 
ATGCTGCAGC TCAGTACCAT TGAGGTGGCC ACCCAGCTGT CAATGAGGGA CTTTGATTTG 
TTTCGTAATA TTGAACCGAC TGAGTACATC GATGACCTTT TTAAGTTAAA TTCCAAAACA 
GGAAATACTC ATTTGAAGAG GTTTGAGGAC ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAAATTT TAACTGAAGC AAATCAGCTC AAACGAATGA AGATTATTAA GCATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCATCTGT AGCAAGACTC AGAGGAACTT GGGAAAAGTT ACCAAGCAAA 
TACGAGAAAC ATCTTCAAGA TCTACAAGAC ATTTTTGATC CATCTAGAAA CATGGCAAAG 
TATAGAAATA TTCTTAGTAG TCAAAGTATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGACT CCAAAGTAGA TGGTTTAGTA 
AACTTTGAGA AGTTAAGAAT GATTTCCAAG GAAATCCGCC AAGTTGTTCG AATGACTTCT 
GCTAACATGG ACCCAGCTAT GATGTTTCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCACTGAGTC AAGGAAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA 
AAAAGGGCAC GCCGCAGCTC TCTGCTTAAT GCCAAGAAGC TATATGAGGA TGCCCAAATG 
GCAAGGAAGG TGAAGCAGTA TCTTTCCAGT CTCGATGTAG AGACAGATGA GGAGAAGTTC 
CAGATGATGT CATTACAGTG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAGTGAG 
AAAAGATCAG CCAAATCATC TGAAATGTCT CCAGTGCCTA TGAGGTCAGC TGGCCAAACA 
ACTAAAGCCC ACTTGCATCA ACCCCACAGA GTAAGCCAGG TGCTTCAGGT GCCAGCTGTT 
AATTTGCACC CCATCAGGAA GAAGGGACAA ACAAAAGACC CTGCACTGAA TACAAGTTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA ATAAGTGGTA AGAAGCATAC AGAAGACACT 
ATTTCTGTGG CGTCATCTTT ACATTCTAGT CCTCCTGCAT CTCCTCAAGG CTCCCCTCAC 
AAAGGTTACA CACTTATTCC ATCAGCTAAA TCTGACAACT TGTCTGACTC CAGCCATAGT 
GAGATTTCTT CACGGTCCAG CATCGTGAGC AATTGTTCTG TTGACTCCAT GTCTGCAGCT 
CTACAGGATG AACGGTGTTC CTCTCAGGCC CTGGCAGTCC CTGAATCCAC TGGGGCATTG 
GAAAAGACAG AGCACGCTTC AGGGATAGGA GATCATAGTC AACATGGCCC TGGGTGGACA 
CTCTTGAAGC CATCTCTAAT CAAGTGTTTA GCTGTCTCAT CGTCTGTGAG CAATGAAGAG 
ATTTCTCAAG AGCATATCAT TATAGAAGCA GCTGACAGTG GTCGTGGAAG TTGGACTTCG 
TGTTCAAGCA GCTCCCATGA CAACTTCCAA AGCCTTCCAA ACCCAAAAAG CTGGGATTTT 
TTGAACTCTT ACAGACATAC CCATTTGGAT GACCCCATTG CTGAAGTTGA ACCCACTGAC 
TCTGAGCCCT ATTCCTGTTC TAAAAGCTGC TCTAGAACTT GTGGGCAGTG TAAAGGAAGC 
CTAGAGAGAA AGAGTTGGAC CTCCTCCAGT TCTCTGTCTG ACACGTATGA ACCAAACTAT 
GGGACAGTTA AACAGAGAGT ATTGGAGAGC ACCCCAGCTG AGTCATCTGA AGGCTTGGAC 
CCCAAGGATG CCACTGACCC AGTTTATAAA ACTGTCACTT CAAGTACAGA AAAGGGCTTG 
ATTGAAAATG AACAAGTTTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_001164387.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157859.1 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 3, mRNA.

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CloneID	OHu03516
Clone ID Related Accession (Same CDS sequence)	NM_001164387.1 , NM_001164387.2
Accession Version	NM_001164387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, AF394782.1, AV726501.2, BU568060.1, DA817493.1 and AL832899.1. On Jun 2, 2019 this sequence version replaced NM_001164387.1. Transcript Variant: This variant (3) includes an alternate in-frame exon in the central coding region but lacks another alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF394782.1, SRR1803613.52321.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGC TGTTGGTTTT TATTATATCC CTGATCAAGT TATAAGAGTT 
TTCAAAGTGG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACCAC AGCTAAAGAA 
GTAGTTTTTC ATGCTGTTCA TGAATTTGGT TTGACCGGTG CATCCGACAC ATATTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTC ATAAAACAGA GAAGACTTCC AGATCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACTTAAAAAA TAACATGGAA 
ACAGAAACCT TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTATCC 
ATGCTGCAGC TCAGTACCAT TGAGGTGGCC ACCCAGCTGT CAATGAGGGA CTTTGATTTG 
TTTCGTAATA TTGAACCGAC TGAGTACATC GATGACCTTT TTAAGTTAAA TTCCAAAACA 
GGAAATACTC ATTTGAAGAG GTTTGAGGAC ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAAATTT TAACTGAAGC AAATCAGCTC AAACGAATGA AGATTATTAA GCATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCATCTGT AGCAAGACTC AGAGGAACTT GGGAAAAGTT ACCAAGCAAA 
TACGAGAAAC ATCTTCAAGA TCTACAAGAC ATTTTTGATC CATCTAGAAA CATGGCAAAG 
TATAGAAATA TTCTTAGTAG TCAAAGTATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGACT CCAAAGTAGA TGGTTTAGTA 
AACTTTGAGA AGTTAAGAAT GATTTCCAAG GAAATCCGCC AAGTTGTTCG AATGACTTCT 
GCTAACATGG ACCCAGCTAT GATGTTTCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCACTGAGTC AAGGAAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA 
AAAAGGGCAC GCCGCAGCTC TCTGCTTAAT GCCAAGAAGC TATATGAGGA TGCCCAAATG 
GCAAGGAAGG TGAAGCAGTA TCTTTCCAGT CTCGATGTAG AGACAGATGA GGAGAAGTTC 
CAGATGATGT CATTACAGTG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAGTGAG 
AAAAGATCAG CCAAATCATC TGAAATGTCT CCAGTGCCTA TGAGGTCAGC TGGCCAAACA 
ACTAAAGCCC ACTTGCATCA ACCCCACAGA GTAAGCCAGG TGCTTCAGGT GCCAGCTGTT 
AATTTGCACC CCATCAGGAA GAAGGGACAA ACAAAAGACC CTGCACTGAA TACAAGTTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA ATAAGTGGTA AGAAGCATAC AGAAGACACT 
ATTTCTGTGG CGTCATCTTT ACATTCTAGT CCTCCTGCAT CTCCTCAAGG CTCCCCTCAC 
AAAGGTTACA CACTTATTCC ATCAGCTAAA TCTGACAACT TGTCTGACTC CAGCCATAGT 
GAGATTTCTT CACGGTCCAG CATCGTGAGC AATTGTTCTG TTGACTCCAT GTCTGCAGCT 
CTACAGGATG AACGGTGTTC CTCTCAGGCC CTGGCAGTCC CTGAATCCAC TGGGGCATTG 
GAAAAGACAG AGCACGCTTC AGGGATAGGA GATCATAGTC AACATGGCCC TGGGTGGACA 
CTCTTGAAGC CATCTCTAAT CAAGTGTTTA GCTGTCTCAT CGTCTGTGAG CAATGAAGAG 
ATTTCTCAAG AGCATATCAT TATAGAAGCA GCTGACAGTG GTCGTGGAAG TTGGACTTCG 
TGTTCAAGCA GCTCCCATGA CAACTTCCAA AGCCTTCCAA ACCCAAAAAG CTGGGATTTT 
TTGAACTCTT ACAGACATAC CCATTTGGAT GACCCCATTG CTGAAGTTGA ACCCACTGAC 
TCTGAGCCCT ATTCCTGTTC TAAAAGCTGC TCTAGAACTT GTGGGCAGTG TAAAGGAAGC 
CTAGAGAGAA AGAGTTGGAC CTCCTCCAGT TCTCTGTCTG ACACGTATGA ACCAAACTAT 
GGGACAGTTA AACAGAGAGT ATTGGAGAGC ACCCCAGCTG AGTCATCTGA AGGCTTGGAC 
CCCAAGGATG CCACTGACCC AGTTTATAAA ACTGTCACTT CAAGTACAGA AAAGGGCTTG 
ATTGAAAATG AACAAGTTTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157859.1
CDS	200..4729
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVGFYYIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKDATDPVYKTVTSSTEKGLIENEQVSAV

Target ORF information:

RefSeq Version	NM_001164387.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164387.2

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGC TGTTGGTTTT TATTATATCC CTGATCAAGT TATAAGAGTT 
TTCAAAGTGG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACCAC AGCTAAAGAA 
GTAGTTTTTC ATGCTGTTCA TGAATTTGGT TTGACCGGTG CATCCGACAC ATATTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTC ATAAAACAGA GAAGACTTCC AGATCAGTTC 
TCCAAATTAG CTGATAGAAT TCAACTCAAT GGAAGGTATT ACTTAAAAAA TAACATGGAA 
ACAGAAACCT TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTATCC 
ATGCTGCAGC TCAGTACCAT TGAGGTGGCC ACCCAGCTGT CAATGAGGGA CTTTGATTTG 
TTTCGTAATA TTGAACCGAC TGAGTACATC GATGACCTTT TTAAGTTAAA TTCCAAAACA 
GGAAATACTC ATTTGAAGAG GTTTGAGGAC ATTGTAAACC AAGAGACATT CTGGGTTGCC 
TCAGAAATTT TAACTGAAGC AAATCAGCTC AAACGAATGA AGATTATTAA GCATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGCTTGAACC TGGCATCTGT AGCAAGACTC AGAGGAACTT GGGAAAAGTT ACCAAGCAAA 
TACGAGAAAC ATCTTCAAGA TCTACAAGAC ATTTTTGATC CATCTAGAAA CATGGCAAAG 
TATAGAAATA TTCTTAGTAG TCAAAGTATG CAGCCTCCAA TTATTCCACT CTTCCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGACT CCAAAGTAGA TGGTTTAGTA 
AACTTTGAGA AGTTAAGAAT GATTTCCAAG GAAATCCGCC AAGTTGTTCG AATGACTTCT 
GCTAACATGG ACCCAGCTAT GATGTTTCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCACTGAGTC AAGGAAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA 
AAAAGGGCAC GCCGCAGCTC TCTGCTTAAT GCCAAGAAGC TATATGAGGA TGCCCAAATG 
GCAAGGAAGG TGAAGCAGTA TCTTTCCAGT CTCGATGTAG AGACAGATGA GGAGAAGTTC 
CAGATGATGT CATTACAGTG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAGTGAG 
AAAAGATCAG CCAAATCATC TGAAATGTCT CCAGTGCCTA TGAGGTCAGC TGGCCAAACA 
ACTAAAGCCC ACTTGCATCA ACCCCACAGA GTAAGCCAGG TGCTTCAGGT GCCAGCTGTT 
AATTTGCACC CCATCAGGAA GAAGGGACAA ACAAAAGACC CTGCACTGAA TACAAGTTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA ATAAGTGGTA AGAAGCATAC AGAAGACACT 
ATTTCTGTGG CGTCATCTTT ACATTCTAGT CCTCCTGCAT CTCCTCAAGG CTCCCCTCAC 
AAAGGTTACA CACTTATTCC ATCAGCTAAA TCTGACAACT TGTCTGACTC CAGCCATAGT 
GAGATTTCTT CACGGTCCAG CATCGTGAGC AATTGTTCTG TTGACTCCAT GTCTGCAGCT 
CTACAGGATG AACGGTGTTC CTCTCAGGCC CTGGCAGTCC CTGAATCCAC TGGGGCATTG 
GAAAAGACAG AGCACGCTTC AGGGATAGGA GATCATAGTC AACATGGCCC TGGGTGGACA 
CTCTTGAAGC CATCTCTAAT CAAGTGTTTA GCTGTCTCAT CGTCTGTGAG CAATGAAGAG 
ATTTCTCAAG AGCATATCAT TATAGAAGCA GCTGACAGTG GTCGTGGAAG TTGGACTTCG 
TGTTCAAGCA GCTCCCATGA CAACTTCCAA AGCCTTCCAA ACCCAAAAAG CTGGGATTTT 
TTGAACTCTT ACAGACATAC CCATTTGGAT GACCCCATTG CTGAAGTTGA ACCCACTGAC 
TCTGAGCCCT ATTCCTGTTC TAAAAGCTGC TCTAGAACTT GTGGGCAGTG TAAAGGAAGC 
CTAGAGAGAA AGAGTTGGAC CTCCTCCAGT TCTCTGTCTG ACACGTATGA ACCAAACTAT 
GGGACAGTTA AACAGAGAGT ATTGGAGAGC ACCCCAGCTG AGTCATCTGA AGGCTTGGAC 
CCCAAGGATG CCACTGACCC AGTTTATAAA ACTGTCACTT CAAGTACAGA AAAGGGCTTG 
ATTGAAAATG AACAAGTTTC AGCAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_001164388.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157860.1 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu03517
Clone ID Related Accession (Same CDS sequence)	NM_001164388.1 , NM_001164388.2
Accession Version	NM_001164388.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, BC133703.1, AL832899.1 and DA817493.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The resulting protein (isoform 4) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.125598.1, BC133703.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGA 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGC 
AGCTCTCTGC TTAATGCCAA GAAGCTATAT GAGGATGCCC AAATGGCAAG GAAGGTGAAG 
CAGTATCTTT CCAGTCTCGA TGTAGAGACA GATGAGGAGA AGTTCCAGAT GATGTCATTA 
CAGTGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA GTGAGAAAAG ATCAGCCAAA 
TCATCTGAAA TGTCTCCAGT GCCTATGAGG TCAGCTGGCC AAACAACTAA AGCCCACTTG 
CATCAACCCC ACAGAGTAAG CCAGGTGCTT CAGGTGCCAG CTGTTAATTT GCACCCCATC 
AGGAAGAAGG GACAAACAAA AGACCCTGCA CTGAATACAA GTTTACCTCA GAAAGTTTTA 
GGAACAACTG AAGAAATAAG TGGTAAGAAG CATACAGAAG ACACTATTTC TGTGGCGTCA 
TCTTTACATT CTAGTCCTCC TGCATCTCCT CAAGGCTCCC CTCACAAAGG TTACACACTT 
ATTCCATCAG CTAAATCTGA CAACTTGTCT GACTCCAGCC ATAGTGAGAT TTCTTCACGG 
TCCAGCATCG TGAGCAATTG TTCTGTTGAC TCCATGTCTG CAGCTCTACA GGATGAACGG 
TGTTCCTCTC AGGCCCTGGC AGTCCCTGAA TCCACTGGGG CATTGGAAAA GACAGAGCAC 
GCTTCAGGGA TAGGAGATCA TAGTCAACAT GGCCCTGGGT GGACACTCTT GAAGCCATCT 
CTAATCAAGT GTTTAGCTGT CTCATCGTCT GTGAGCAATG AAGAGATTTC TCAAGAGCAT 
ATCATTATAG AAGCAGCTGA CAGTGGTCGT GGAAGTTGGA CTTCGTGTTC AAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCCAAACCCA AAAAGCTGGG ATTTTTTGAA CTCTTACAGA 
CATACCCATT TGGATGACCC CATTGCTGAA GTTGAACCCA CTGACTCTGA GCCCTATTCC 
TGTTCTAAAA GCTGCTCTAG AACTTGTGGG CAGTGTAAAG GAAGCCTAGA GAGAAAGAGT 
TGGACCTCCT CCAGTTCTCT GTCTGACACG TATGAACCAA ACTATGGGAC AGTTAAACAG 
AGAGTATTGG AGAGCACCCC AGCTGAGTCA TCTGAAGGCT TGGACCCCAA GGATGCCACT 
GACCCAGTTT ATAAAACTGT CACTTCAAGT ACAGAAAAGG GCTTGATTGA AAATGAACAA 
GTTTCAGCAG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157860.1
CDS	207..4721
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKDATDPVYKTVTSSTEKGLIENEQVSAV

Target ORF information:

RefSeq Version	NM_001164388.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164388.1

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGA 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGC 
AGCTCTCTGC TTAATGCCAA GAAGCTATAT GAGGATGCCC AAATGGCAAG GAAGGTGAAG 
CAGTATCTTT CCAGTCTCGA TGTAGAGACA GATGAGGAGA AGTTCCAGAT GATGTCATTA 
CAGTGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA GTGAGAAAAG ATCAGCCAAA 
TCATCTGAAA TGTCTCCAGT GCCTATGAGG TCAGCTGGCC AAACAACTAA AGCCCACTTG 
CATCAACCCC ACAGAGTAAG CCAGGTGCTT CAGGTGCCAG CTGTTAATTT GCACCCCATC 
AGGAAGAAGG GACAAACAAA AGACCCTGCA CTGAATACAA GTTTACCTCA GAAAGTTTTA 
GGAACAACTG AAGAAATAAG TGGTAAGAAG CATACAGAAG ACACTATTTC TGTGGCGTCA 
TCTTTACATT CTAGTCCTCC TGCATCTCCT CAAGGCTCCC CTCACAAAGG TTACACACTT 
ATTCCATCAG CTAAATCTGA CAACTTGTCT GACTCCAGCC ATAGTGAGAT TTCTTCACGG 
TCCAGCATCG TGAGCAATTG TTCTGTTGAC TCCATGTCTG CAGCTCTACA GGATGAACGG 
TGTTCCTCTC AGGCCCTGGC AGTCCCTGAA TCCACTGGGG CATTGGAAAA GACAGAGCAC 
GCTTCAGGGA TAGGAGATCA TAGTCAACAT GGCCCTGGGT GGACACTCTT GAAGCCATCT 
CTAATCAAGT GTTTAGCTGT CTCATCGTCT GTGAGCAATG AAGAGATTTC TCAAGAGCAT 
ATCATTATAG AAGCAGCTGA CAGTGGTCGT GGAAGTTGGA CTTCGTGTTC AAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCCAAACCCA AAAAGCTGGG ATTTTTTGAA CTCTTACAGA 
CATACCCATT TGGATGACCC CATTGCTGAA GTTGAACCCA CTGACTCTGA GCCCTATTCC 
TGTTCTAAAA GCTGCTCTAG AACTTGTGGG CAGTGTAAAG GAAGCCTAGA GAGAAAGAGT 
TGGACCTCCT CCAGTTCTCT GTCTGACACG TATGAACCAA ACTATGGGAC AGTTAAACAG 
AGAGTATTGG AGAGCACCCC AGCTGAGTCA TCTGAAGGCT TGGACCCCAA GGATGCCACT 
GACCCAGTTT ATAAAACTGT CACTTCAAGT ACAGAAAAGG GCTTGATTGA AAATGAACAA 
GTTTCAGCAG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_001164388.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157860.1 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 4, mRNA.

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CloneID	OHu03517
Clone ID Related Accession (Same CDS sequence)	NM_001164388.1 , NM_001164388.2
Accession Version	NM_001164388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, BC133703.1, AL832899.1 and DA817493.1. On Jun 2, 2019 this sequence version replaced NM_001164388.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The resulting protein (isoform 4) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.125598.1, BC133703.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGA 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGC 
AGCTCTCTGC TTAATGCCAA GAAGCTATAT GAGGATGCCC AAATGGCAAG GAAGGTGAAG 
CAGTATCTTT CCAGTCTCGA TGTAGAGACA GATGAGGAGA AGTTCCAGAT GATGTCATTA 
CAGTGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA GTGAGAAAAG ATCAGCCAAA 
TCATCTGAAA TGTCTCCAGT GCCTATGAGG TCAGCTGGCC AAACAACTAA AGCCCACTTG 
CATCAACCCC ACAGAGTAAG CCAGGTGCTT CAGGTGCCAG CTGTTAATTT GCACCCCATC 
AGGAAGAAGG GACAAACAAA AGACCCTGCA CTGAATACAA GTTTACCTCA GAAAGTTTTA 
GGAACAACTG AAGAAATAAG TGGTAAGAAG CATACAGAAG ACACTATTTC TGTGGCGTCA 
TCTTTACATT CTAGTCCTCC TGCATCTCCT CAAGGCTCCC CTCACAAAGG TTACACACTT 
ATTCCATCAG CTAAATCTGA CAACTTGTCT GACTCCAGCC ATAGTGAGAT TTCTTCACGG 
TCCAGCATCG TGAGCAATTG TTCTGTTGAC TCCATGTCTG CAGCTCTACA GGATGAACGG 
TGTTCCTCTC AGGCCCTGGC AGTCCCTGAA TCCACTGGGG CATTGGAAAA GACAGAGCAC 
GCTTCAGGGA TAGGAGATCA TAGTCAACAT GGCCCTGGGT GGACACTCTT GAAGCCATCT 
CTAATCAAGT GTTTAGCTGT CTCATCGTCT GTGAGCAATG AAGAGATTTC TCAAGAGCAT 
ATCATTATAG AAGCAGCTGA CAGTGGTCGT GGAAGTTGGA CTTCGTGTTC AAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCCAAACCCA AAAAGCTGGG ATTTTTTGAA CTCTTACAGA 
CATACCCATT TGGATGACCC CATTGCTGAA GTTGAACCCA CTGACTCTGA GCCCTATTCC 
TGTTCTAAAA GCTGCTCTAG AACTTGTGGG CAGTGTAAAG GAAGCCTAGA GAGAAAGAGT 
TGGACCTCCT CCAGTTCTCT GTCTGACACG TATGAACCAA ACTATGGGAC AGTTAAACAG 
AGAGTATTGG AGAGCACCCC AGCTGAGTCA TCTGAAGGCT TGGACCCCAA GGATGCCACT 
GACCCAGTTT ATAAAACTGT CACTTCAAGT ACAGAAAAGG GCTTGATTGA AAATGAACAA 
GTTTCAGCAG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157860.1
CDS	200..4714
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHSQHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKDATDPVYKTVTSSTEKGLIENEQVSAV

Target ORF information:

RefSeq Version	NM_001164388.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164388.2

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGA 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGC 
AGCTCTCTGC TTAATGCCAA GAAGCTATAT GAGGATGCCC AAATGGCAAG GAAGGTGAAG 
CAGTATCTTT CCAGTCTCGA TGTAGAGACA GATGAGGAGA AGTTCCAGAT GATGTCATTA 
CAGTGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA GTGAGAAAAG ATCAGCCAAA 
TCATCTGAAA TGTCTCCAGT GCCTATGAGG TCAGCTGGCC AAACAACTAA AGCCCACTTG 
CATCAACCCC ACAGAGTAAG CCAGGTGCTT CAGGTGCCAG CTGTTAATTT GCACCCCATC 
AGGAAGAAGG GACAAACAAA AGACCCTGCA CTGAATACAA GTTTACCTCA GAAAGTTTTA 
GGAACAACTG AAGAAATAAG TGGTAAGAAG CATACAGAAG ACACTATTTC TGTGGCGTCA 
TCTTTACATT CTAGTCCTCC TGCATCTCCT CAAGGCTCCC CTCACAAAGG TTACACACTT 
ATTCCATCAG CTAAATCTGA CAACTTGTCT GACTCCAGCC ATAGTGAGAT TTCTTCACGG 
TCCAGCATCG TGAGCAATTG TTCTGTTGAC TCCATGTCTG CAGCTCTACA GGATGAACGG 
TGTTCCTCTC AGGCCCTGGC AGTCCCTGAA TCCACTGGGG CATTGGAAAA GACAGAGCAC 
GCTTCAGGGA TAGGAGATCA TAGTCAACAT GGCCCTGGGT GGACACTCTT GAAGCCATCT 
CTAATCAAGT GTTTAGCTGT CTCATCGTCT GTGAGCAATG AAGAGATTTC TCAAGAGCAT 
ATCATTATAG AAGCAGCTGA CAGTGGTCGT GGAAGTTGGA CTTCGTGTTC AAGCAGCTCC 
CATGACAACT TCCAAAGCCT TCCAAACCCA AAAAGCTGGG ATTTTTTGAA CTCTTACAGA 
CATACCCATT TGGATGACCC CATTGCTGAA GTTGAACCCA CTGACTCTGA GCCCTATTCC 
TGTTCTAAAA GCTGCTCTAG AACTTGTGGG CAGTGTAAAG GAAGCCTAGA GAGAAAGAGT 
TGGACCTCCT CCAGTTCTCT GTCTGACACG TATGAACCAA ACTATGGGAC AGTTAAACAG 
AGAGTATTGG AGAGCACCCC AGCTGAGTCA TCTGAAGGCT TGGACCCCAA GGATGCCACT 
GACCCAGTTT ATAAAACTGT CACTTCAAGT ACAGAAAAGG GCTTGATTGA AAATGAACAA 
GTTTCAGCAG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_001164389.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157861.1 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 5, mRNA.

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CloneID	OHu03520
Clone ID Related Accession (Same CDS sequence)	NM_001164389.1 , NM_001164389.2
Accession Version	NM_001164389.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, AF478469.1, BX117281.1 and AW204176.1. Transcript Variant: This variant (5) differs in the 3' UTR and 3' coding region, compared to variant 1. It encodes isoform 5 which has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF478469.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGAAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGCAGCT CTCTGCTTAA TGCCAAGAAG 
CTATATGAGG ATGCCCAAAT GGCAAGGAAG GTGAAGCAGT ATCTTTCCAG TCTCGATGTA 
GAGACAGATG AGGAGAAGTT CCAGATGATG TCATTACAGT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CTGAAATGTC TCCAGTGCCT 
ATGAGGTCAG CTGGCCAAAC AACTAAAGCC CACTTGCATC AACCCCACAG AGTAAGCCAG 
GTGCTTCAGG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AACAAAAGAC 
CCTGCACTGA ATACAAGTTT ACCTCAGAAA GTTTTAGGAA CAACTGAAGA AATAAGTGGT 
AAGAAGCATA CAGAAGACAC TATTTCTGTG GCGTCATCTT TACATTCTAG TCCTCCTGCA 
TCTCCTCAAG GCTCCCCTCA CAAAGTTGGC AGTATCATAT CTGATCACAG CAGCAAGATC 
AGTGGCCAGA GCTGCCCCGG AATAGGTGGG GCTTACTTGC AAAAGAAAAT CCTTCAGATT 
ACCAGGAGCA CAGCCAAAAG AACAGACAGT ACTGAGAAGG CCACTGAGGA GAATAGAGAC 
AGGACAAGTT GTGAGAACAC AACCAGAAAA AGAATGACAT CCCCGTTTAG AAGACTTAGG 
GAAAGAATGC TTTCAAGGGA GAGACTAGTC AACAGCCAAA AAGAGGACAC AGATCACAAT 
CAAGCTACAG AAAGCTGTGA GAAAGTGAAG GATGTTGGAA GCAACATCAA AGATGAGAAA 
GGGAGTGCCA TCTTCAATTC AAATAGCCAA GGCAACAGTA ACACTTTAAA CTGCTTCTAT 
ACGCGATTCA AGTCTAAAAG GAGGAAGACA CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157861.1
CDS	207..4382
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKVGSIISDHSSKISGQSCPGIGGAYLQKKILQITRSTAKRTDSTEKATEENRDRTSCENTTRKRMTSPFRRLRERMLSRERLVNSQKEDTDHNQATESCEKVKDVGSNIKDEKGSAIFNSNSQGNSNTLNCFYTRFKSKRRKTL

Target ORF information:

RefSeq Version	NM_001164389.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164389.1

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGAAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGCAGCT CTCTGCTTAA TGCCAAGAAG 
CTATATGAGG ATGCCCAAAT GGCAAGGAAG GTGAAGCAGT ATCTTTCCAG TCTCGATGTA 
GAGACAGATG AGGAGAAGTT CCAGATGATG TCATTACAGT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CTGAAATGTC TCCAGTGCCT 
ATGAGGTCAG CTGGCCAAAC AACTAAAGCC CACTTGCATC AACCCCACAG AGTAAGCCAG 
GTGCTTCAGG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AACAAAAGAC 
CCTGCACTGA ATACAAGTTT ACCTCAGAAA GTTTTAGGAA CAACTGAAGA AATAAGTGGT 
AAGAAGCATA CAGAAGACAC TATTTCTGTG GCGTCATCTT TACATTCTAG TCCTCCTGCA 
TCTCCTCAAG GCTCCCCTCA CAAAGTTGGC AGTATCATAT CTGATCACAG CAGCAAGATC 
AGTGGCCAGA GCTGCCCCGG AATAGGTGGG GCTTACTTGC AAAAGAAAAT CCTTCAGATT 
ACCAGGAGCA CAGCCAAAAG AACAGACAGT ACTGAGAAGG CCACTGAGGA GAATAGAGAC 
AGGACAAGTT GTGAGAACAC AACCAGAAAA AGAATGACAT CCCCGTTTAG AAGACTTAGG 
GAAAGAATGC TTTCAAGGGA GAGACTAGTC AACAGCCAAA AAGAGGACAC AGATCACAAT 
CAAGCTACAG AAAGCTGTGA GAAAGTGAAG GATGTTGGAA GCAACATCAA AGATGAGAAA 
GGGAGTGCCA TCTTCAATTC AAATAGCCAA GGCAACAGTA ACACTTTAAA CTGCTTCTAT 
ACGCGATTCA AGTCTAAAAG GAGGAAGACA CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( NM_001164389.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157861.1 Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 5, mRNA.

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CloneID	OHu03520
Clone ID Related Accession (Same CDS sequence)	NM_001164389.1 , NM_001164389.2
Accession Version	NM_001164389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 6 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF478567.1, AF478469.1, BX117281.1, AW204176.1 and AC008497.7. On Jun 2, 2019 this sequence version replaced NM_001164389.1. Transcript Variant: This variant (5) differs in the 3' UTR and 3' coding region, compared to variant 1. It encodes isoform 5 which has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF478469.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGAAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGCAGCT CTCTGCTTAA TGCCAAGAAG 
CTATATGAGG ATGCCCAAAT GGCAAGGAAG GTGAAGCAGT ATCTTTCCAG TCTCGATGTA 
GAGACAGATG AGGAGAAGTT CCAGATGATG TCATTACAGT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CTGAAATGTC TCCAGTGCCT 
ATGAGGTCAG CTGGCCAAAC AACTAAAGCC CACTTGCATC AACCCCACAG AGTAAGCCAG 
GTGCTTCAGG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AACAAAAGAC 
CCTGCACTGA ATACAAGTTT ACCTCAGAAA GTTTTAGGAA CAACTGAAGA AATAAGTGGT 
AAGAAGCATA CAGAAGACAC TATTTCTGTG GCGTCATCTT TACATTCTAG TCCTCCTGCA 
TCTCCTCAAG GCTCCCCTCA CAAAGTTGGC AGTATCATAT CTGATCACAG CAGCAAGATC 
AGTGGCCAGA GCTGCCCCGG AATAGGTGGG GCTTACTTGC AAAAGAAAAT CCTTCAGATT 
ACCAGGAGCA CAGCCAAAAG AACAGACAGT ACTGAGAAGG CCACTGAGGA GAATAGAGAC 
AGGACAAGTT GTGAGAACAC AACCAGAAAA AGAATGACAT CCCCGTTTAG AAGACTTAGG 
GAAAGAATGC TTTCAAGGGA GAGACTAGTC AACAGCCAAA AAGAGGACAC AGATCACAAT 
CAAGCTACAG AAAGCTGTGA GAAAGTGAAG GATGTTGGAA GCAACATCAA AGATGAGAAA 
GGGAGTGCCA TCTTCAATTC AAATAGCCAA GGCAACAGTA ACACTTTAAA CTGCTTCTAT 
ACGCGATTCA AGTCTAAAAG GAGGAAGACA CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157861.1
CDS	200..4375
Translation	MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQEKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKVGSIISDHSSKISGQSCPGIGGAYLQKKILQITRSTAKRTDSTEKATEENRDRTSCENTTRKRMTSPFRRLRERMLSRERLVNSQKEDTDHNQATESCEKVKDVGSNIKDEKGSAIFNSNSQGNSNTLNCFYTRFKSKRRKTL

Target ORF information:

RefSeq Version	NM_001164389.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164389.2

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC TGGCGCTAGG CAGGCGTTGA GGAAGAAGCC ACCCGAGCGG 
ACTCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAGGTTC TCTTTTGTTC AGAAACGATT GCCAGATGTT GGTATATCCT ACTTTCTGGA 
TCTGTGCTTG TGAAAGGCTC CATGGTCTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
GCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA ATCCCGAAGA 
AGATTTCGGA AAATTAACTA TAAAGGAGAG CGCCAAACCA TTACTGATGA TGTGGAGGTT 
AACAGCTATC TTTCTCTTCC AGCTGATCTT ACCAAGATGC ATCTCACAGA AAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATTGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAGAGTG AGGTAGGAGA TGTAGATTTG 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAGGATGACG AAGAGGAAGA TGAAGAGATT 
GATCGAACAG ATCCATTGCA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA AAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTCCCTGCA 
TTTGCAAACA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGAAAA TTTGTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GATAAGCAGT ACATGCATGG AATTGTCAGG 
ACTAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCCCAGC AAGATTATTG GAGAATTTTA 
AACCATGTGG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGGG AACTAGACCG GAGTGGAACC AGGAAAGGAC ACATTGTGAT CAAGGCAACA 
CCTGAGCGTC TCATAATGCA TTTAATAGAA GAACATTCCA TCGTGGATCC AACTTATATA 
GAAGATTTTC TATTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAGAT CGACAGCTTA AGAGATAAGG TGACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGACCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAG ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AAAAGGCTTC CCGCGAGTCC 
CCTCTACAAT TCAGCCTTAA TGGAGGGAGT GAGAAGGGAT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTG GTAGCAAAGC TGCTGATTCA GGACTGAAAC GTGGTGATCA GATTATGGAA 
GTAAATGGAC AAAACTTTGA GAATATTACA TTTATGAAAG CCGTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA GTTACTTTTT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CTATCCAGCA TGTGCCAGGA GATATTGAAC AGACATCACA GGAGAAAGGA 
AGTAAGAAAG TTAAAGCAAA TACTGTTTCA GGTGGAAGAA ACAAAATCAG GAAGATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAGCTATTTA GTGATGGAGG CCTAAGCCAA 
TCACAAGATG ACAGCATTGT GGGAACAAGG CACTGTAGGC ATAGTCTGGC TATAATGCCC 
ATCCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCCTGC AGCCTACCAC CAGTATGTTG 
GATTTTTCCA ATCCTTCAGA TATCCCTGAT CAAGTTATAA GAGTTTTCAA AGTGGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACCACAGCTA AAGAAGTAGT TTTTCATGCT 
GTTCATGAAT TTGGTTTGAC CGGTGCATCC GACACATATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAGAGAAGA CTTCCAGATC AGTTCTCCAA ATTAGCTGAT 
AGAATTCAAC TCAATGGAAG GTATTACTTA AAAAATAACA TGGAAACAGA AACCTTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACCCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAA 
CCGACTGAGT ACATCGATGA CCTTTTTAAG TTAAATTCCA AAACAGGAAA TACTCATTTG 
AAGAGGTTTG AGGACATTGT AAACCAAGAG ACATTCTGGG TTGCCTCAGA AATTTTAACT 
GAAGCAAATC AGCTCAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAA GACTCAGAGG AACTTGGGAA AAGTTACCAA GCAAATACGA GAAACATCTT 
CAAGATCTAC AAGACATTTT TGATCCATCT AGAAACATGG CAAAGTATAG AAATATTCTT 
AGTAGTCAAA GTATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTAAACTT TGAGAAGTTA 
AGAATGATTT CCAAGGAAAT CCGCCAAGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGAAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGCAGCT CTCTGCTTAA TGCCAAGAAG 
CTATATGAGG ATGCCCAAAT GGCAAGGAAG GTGAAGCAGT ATCTTTCCAG TCTCGATGTA 
GAGACAGATG AGGAGAAGTT CCAGATGATG TCATTACAGT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CTGAAATGTC TCCAGTGCCT 
ATGAGGTCAG CTGGCCAAAC AACTAAAGCC CACTTGCATC AACCCCACAG AGTAAGCCAG 
GTGCTTCAGG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AACAAAAGAC 
CCTGCACTGA ATACAAGTTT ACCTCAGAAA GTTTTAGGAA CAACTGAAGA AATAAGTGGT 
AAGAAGCATA CAGAAGACAC TATTTCTGTG GCGTCATCTT TACATTCTAG TCCTCCTGCA 
TCTCCTCAAG GCTCCCCTCA CAAAGTTGGC AGTATCATAT CTGATCACAG CAGCAAGATC 
AGTGGCCAGA GCTGCCCCGG AATAGGTGGG GCTTACTTGC AAAAGAAAAT CCTTCAGATT 
ACCAGGAGCA CAGCCAAAAG AACAGACAGT ACTGAGAAGG CCACTGAGGA GAATAGAGAC 
AGGACAAGTT GTGAGAACAC AACCAGAAAA AGAATGACAT CCCCGTTTAG AAGACTTAGG 
GAAAGAATGC TTTCAAGGGA GAGACTAGTC AACAGCCAAA AAGAGGACAC AGATCACAAT 
CAAGCTACAG AAAGCTGTGA GAAAGTGAAG GATGTTGGAA GCAACATCAA AGATGAGAAA 
GGGAGTGCCA TCTTCAATTC AAATAGCCAA GGCAACAGTA ACACTTTAAA CTGCTTCTAT 
ACGCGATTCA AGTCTAAAAG GAGGAAGACA CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RAPGEF6
Entrez Gene ID	51735
Full Name	Rap guanine nucleotide exchange factor 6
Synonyms	KIA001LB,PDZ-GEF2,PDZGEF2,RA-GEF-2,RAGEF2
General protein information	Preferred Names Rap guanine nucleotide exchange factor 6 Names rap guanine nucleotide exchange factor 6 PDZ domain containing guanine nucleotide exchange factor (GEF) 2 PDZ domain-containing guanine nucleotide exchange factor 2 PDZ domain-containing guanine nucleotide exchange factor I Rap guanine nucleotide exchange factor (GEF) 6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.1
Disorder MIM:	610499

Bos taurus (cattle)	RAPGEF6	XP_003586323.1
Homo sapiens (human)	RAPGEF6	NP_001157858.1
Canis lupus familiaris (dog)	RAPGEF6	XP_003431670.1
Mus musculus (house mouse)	Rapgef6	NP_780467.2
Gallus gallus (chicken)	RAPGEF6	XP_004945062.1
Pan troglodytes (chimpanzee)	RAPGEF6	XP_003339150.1
	RAPGEF6	XP_001101236.2
Rattus norvegicus (Norway rat)	Rapgef6	NP_001100473.1
Danio rerio (zebrafish)	rapgef6	XP_002666140.3
Caenorhabditis elegans (roundworm)	pxf-1	NP_001023389.1

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RAPGEF6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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