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ACER3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ACER3
Entrez Gene ID 55331
Full Name alkaline ceramidase 3
Synonyms APHC, PHCA
General protein information
Preferred Names
alkaline ceramidase 3
Names
alkaline ceramidase 3
alkCDase 3
alkaline CDase 3
alkaline phytoceramidase
phytoceramidase, alkaline
alkaline dihydroceramidase SB89
NP_060837.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q13.5

Summary lac of sum

mRNA and Protein(s)

mRNA Protein Name
XM_011545151 XP_011543453 alkaline ceramidase 3 isoform X1
XM_005274087 XP_005274144 alkaline ceramidase 3 isoform X2
XM_011545152 XP_011543454 alkaline ceramidase 3 isoform X2
XM_005274092 XP_005274149 alkaline ceramidase 3 isoform X3
XM_011545153 XP_011543455 alkaline ceramidase 3 isoform X3
XM_011545154 XP_011543456 alkaline ceramidase 3 isoform X4
NM_018367 NP_060837 alkaline ceramidase 3 isoform a
NM_001300954 NP_001287883 alkaline ceramidase 3 isoform c
NM_001300955 NP_001287884 alkaline ceramidase 3 isoform c
NM_001300953 NP_001287882 alkaline ceramidase 3 isoform b



Homo sapiens (human) ACER3 NP_060837.3
Pan troglodytes (chimpanzee) ACER3 XP_001175032.1
Macaca mulatta (Rhesus monkey) ACER3 XP_001088478.1
Canis lupus familiaris (dog) ACER3 XP_849760.1
Bos taurus (cattle) ACER3 NP_001095755.1
Mus musculus (house mouse) Acer3 NP_079684.2
Rattus norvegicus (Norway rat) Acer3 XP_574497.2
Gallus gallus (chicken) ACER3 XP_417279.1
Danio rerio (zebrafish) LOC555411 XP_005169254.1
Arabidopsis thaliana (thale cress) ATCES1 NP_567660.1
Xenopus (Silurana) tropicalis (western clawed frog) acer3 NP_001106437.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACER3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACER3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu69360 XM_011545151 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu45421 XM_005274087 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu45421 XM_011545152 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu45423 XM_005274092 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu45423 XM_011545153 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu69361 XM_011545154 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu02784 NM_018367 Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu45422 NM_001300954 Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu45422 NM_001300955 Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu69362 NM_001300953 Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu69360
Clone ID Related Accession (Same CDS sequence) XM_011545151
Accession Version XM_011545151.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGGAA CTTTCGAAAG AAGGTACCAC CTATCATAGG TATTACCACA
CAATTTCATG CATGGTGGCA TATTTTAACT GGCCTTGGTT CCTATCTTCA CATCCTTTTC
AGTTTGTATA CAAGAACACT TTACCTGAGA TATAGGCCAA AAGTGAAGTT TCTCTTTGGA
ATCTGGCCAG TGATCCTGTT TGAGCCTCTC AGGAAGCATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543453.1
CDS71..772
Misc Feature(1)92..745(+)
Translation

Target ORF information:

RefSeq Version XM_011545151
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545151

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGGAA CTTTCGAAAG AAGGTACCAC CTATCATAGG TATTACCACA
CAATTTCATG CATGGTGGCA TATTTTAACT GGCCTTGGTT CCTATCTTCA CATCCTTTTC
AGTTTGTATA CAAGAACACT TTACCTGAGA TATAGGCCAA AAGTGAAGTT TCTCTTTGGA
ATCTGGCCAG TGATCCTGTT TGAGCCTCTC AGGAAGCATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45421
Clone ID Related Accession (Same CDS sequence) XM_005274087 , XM_011545152
Accession Version XM_005274087.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 678bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578821606. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGATTATAC CTCCAATGTT CGGTGCAGTT CAGAGTGTTA GAGACGGTCT GGAAAAGCGG 
TACATTGCTT CTTATTTAGC ACTCACAGTG GTAGGAATGG GATCCTGGTG CTTCCACATG
ACTCTGAAAT ATGAAATGCA GCTATTGGAT GAACTCCCAA TGATATACAG CTGTTGCATA
TTTGTGTACT GCATGTTTGA ATGTTTCAAG ATCAAGAACT CAGTAAACTA CCATCTGCTT
TTTACCTTAG TTCTATTCAG TTTAATAGTA ACCACAGTTT ACCTTAAGGT AAAAGAGCCG
ATATTCCATC AGGTCATGTA TGGAATGTTG GTCTTTACAT TAGTACTTCG ATCTATTTAT
ATTGTTACAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274144.1
CDS315..992
Misc Feature(1)315..965(+)
Translation

Target ORF information:

RefSeq Version XM_005274087
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274087

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGATTATAC CTCCAATGTT CGGTGCAGTT CAGAGTGTTA GAGACGGTCT GGAAAAGCGG 
TACATTGCTT CTTATTTAGC ACTCACAGTG GTAGGAATGG GATCCTGGTG CTTCCACATG
ACTCTGAAAT ATGAAATGCA GCTATTGGAT GAACTCCCAA TGATATACAG CTGTTGCATA
TTTGTGTACT GCATGTTTGA ATGTTTCAAG ATCAAGAACT CAGTAAACTA CCATCTGCTT
TTTACCTTAG TTCTATTCAG TTTAATAGTA ACCACAGTTT ACCTTAAGGT AAAAGAGCCG
ATATTCCATC AGGTCATGTA TGGAATGTTG GTCTTTACAT TAGTACTTCG ATCTATTTAT
ATTGTTACAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45421
Clone ID Related Accession (Same CDS sequence) XM_005274087 , XM_011545152
Accession Version XM_011545152.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 678bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGATTATAC CTCCAATGTT CGGTGCAGTT CAGAGTGTTA GAGACGGTCT GGAAAAGCGG 
TACATTGCTT CTTATTTAGC ACTCACAGTG GTAGGAATGG GATCCTGGTG CTTCCACATG
ACTCTGAAAT ATGAAATGCA GCTATTGGAT GAACTCCCAA TGATATACAG CTGTTGCATA
TTTGTGTACT GCATGTTTGA ATGTTTCAAG ATCAAGAACT CAGTAAACTA CCATCTGCTT
TTTACCTTAG TTCTATTCAG TTTAATAGTA ACCACAGTTT ACCTTAAGGT AAAAGAGCCG
ATATTCCATC AGGTCATGTA TGGAATGTTG GTCTTTACAT TAGTACTTCG ATCTATTTAT
ATTGTTACAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543454.1
CDS125..802
Misc Feature(1)125..775(+)
Translation

Target ORF information:

RefSeq Version XM_011545152
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545152

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGATTATAC CTCCAATGTT CGGTGCAGTT CAGAGTGTTA GAGACGGTCT GGAAAAGCGG 
TACATTGCTT CTTATTTAGC ACTCACAGTG GTAGGAATGG GATCCTGGTG CTTCCACATG
ACTCTGAAAT ATGAAATGCA GCTATTGGAT GAACTCCCAA TGATATACAG CTGTTGCATA
TTTGTGTACT GCATGTTTGA ATGTTTCAAG ATCAAGAACT CAGTAAACTA CCATCTGCTT
TTTACCTTAG TTCTATTCAG TTTAATAGTA ACCACAGTTT ACCTTAAGGT AAAAGAGCCG
ATATTCCATC AGGTCATGTA TGGAATGTTG GTCTTTACAT TAGTACTTCG ATCTATTTAT
ATTGTTACAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45423
Clone ID Related Accession (Same CDS sequence) XM_005274092 , XM_011545153
Accession Version XM_005274092.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 498bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530396977. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274149.1
CDS71..568
Misc Feature(1)92..>565(+)
Translation

Target ORF information:

RefSeq Version XM_005274092
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274092

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45423
Clone ID Related Accession (Same CDS sequence) XM_005274092 , XM_011545153
Accession Version XM_011545153.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 498bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543455.1
CDS71..568
Misc Feature(1)92..>565(+)
Translation

Target ORF information:

RefSeq Version XM_011545153
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545153

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69361
Clone ID Related Accession (Same CDS sequence) XM_011545154
Accession Version XM_011545154.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 438bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
ATGAGAGTAC TACAAAAAGG ACGAGTGCGT GGGATACAAG ACAGGAGATC CAATGAAAGA 
GAGAGACATT CATTTTACCC CACAGAAGAG TATTGCTTTG AACTGCCCCT GAGAGCTGAA
GATTGGCCAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543456.1
CDS653..1090
Misc Feature(1)779..1063(+)
Translation

Target ORF information:

RefSeq Version XM_011545154
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545154

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAGAGTAC TACAAAAAGG ACGAGTGCGT GGGATACAAG ACAGGAGATC CAATGAAAGA 
GAGAGACATT CATTTTACCC CACAGAAGAG TATTGCTTTG AACTGCCCCT GAGAGCTGAA
GATTGGCCAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02784
Clone ID Related Accession (Same CDS sequence) NM_018367
Accession Version NM_018367.6 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform a
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG702017.1, AK315000.1, DB461413.1, AP002498.3, AP000752.4 and AI885537.1. On Jul 17, 2014 this sequence version replaced gi:170932466. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF327353.1, AF214454.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGGGT TTATCCATGG CTTAGAGGAC TGGGTTATAC ATCATTGGGT
ATATTTTTAT TGGGATTTTT ATTTTGGAAT ATAGATAACA TATTTTGTGA GTCACTGAGG
AACTTTCGAA AGAAGGTACC ACCTATCATA GGTATTACCA CACAATTTCA TGCATGGTGG
CATATTTTAA CTGGCCTTGG TTCCTATCTT CACATCCTTT TCAGTTTGTA TACAAGAACA
CTTTACCTGA GATATAGGCC AAAAGTGAAG TTTCTCTTTG GAATCTGGCC AGTGATCCTG
TTTGAGCCTC TCAGGAAGCA TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060837.3
CDS105..908
Misc Feature(1)69..71(+)
Misc Feature(2)126..881(+)
Misc Feature(3)189..257(+)
Misc Feature(4)291..356(+)
Misc Feature(5)384..437(+)
Misc Feature(6)456..515(+)
Misc Feature(7)543..611(+)
Misc Feature(8)624..692(+)
Misc Feature(9)750..818(+)
Exon (1)1..207
Gene:ACER3
Gene Synonym:
Exon (2)208..318
Gene:ACER3
Gene Synonym:
Exon (3)319..371
Gene:ACER3
Gene Synonym:
Exon (4)372..424
Gene:ACER3
Gene Synonym:
Exon (5)425..506
Gene:ACER3
Gene Synonym:
Exon (6)507..542
Gene:ACER3
Gene Synonym:
Exon (7)543..601
Gene:ACER3
Gene Synonym:
Exon (8)602..703
Gene:ACER3
Gene Synonym:
Exon (9)704..808
Gene:ACER3
Gene Synonym:
Exon (10)809..854
Gene:ACER3
Gene Synonym:
Exon (11)855..7378
Gene:ACER3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_018367
Organism Homo sapiens (human)
Definition Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018367

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGGGT TTATCCATGG CTTAGAGGAC TGGGTTATAC ATCATTGGGT
ATATTTTTAT TGGGATTTTT ATTTTGGAAT ATAGATAACA TATTTTGTGA GTCACTGAGG
AACTTTCGAA AGAAGGTACC ACCTATCATA GGTATTACCA CACAATTTCA TGCATGGTGG
CATATTTTAA CTGGCCTTGG TTCCTATCTT CACATCCTTT TCAGTTTGTA TACAAGAACA
CTTTACCTGA GATATAGGCC AAAAGTGAAG TTTCTCTTTG GAATCTGGCC AGTGATCCTG
TTTGAGCCTC TCAGGAAGCA TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45422
Clone ID Related Accession (Same CDS sequence) NM_001300954 , NM_001300955
Accession Version NM_001300954.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform c
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG702017.1, BC049837.1, AP000752.4 and AI885537.1. On Jul 17, 2014 this sequence version replaced gi:578821607. Transcript Variant: This variant (3) lacks an internal exon and has another exon in its place compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Variants 3 and 4 both encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC049837.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGATATACA GCTGTTGCAT ATTTGTGTAC TGCATGTTTG AATGTTTCAA GATCAAGAAC 
TCAGTAAACT ACCATCTGCT TTTTACCTTA GTTCTATTCA GTTTAATAGT AACCACAGTT
TACCTTAAGG TAAAAGAGCC GATATTCCAT CAGGTCATGT ATGGAATGTT GGTCTTTACA
TTAGTACTTC GATCTATTTA TATTGTTACA TGGGTTTATC CATGGCTTAG AGGACTGGGT
TATACATCAT TGGGTATATT TTTATTGGGA TTTTTATTTT GGAATATAGA TAACATATTT
TGTGAGTCAC TGAGGAACTT TCGAAAGAAG GTACCACCTA TCATAGGTAT TACCACACAA
TTTCATGCAT GGTGGCATAT TTTAACTGGC CTTGGTTCCT ATCTTCACAT CCTTTTCAGT
TTGTATACAA GAACACTTTA CCTGAGATAT AGGCCAAAAG TGAAGTTTCT CTTTGGAATC
TGGCCAGTGA TCCTGTTTGA GCCTCTCAGG AAGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001287883.1
CDS461..979
Misc Feature(1)428..430(+)
Misc Feature(2)461..952(+)
Exon (1)1..207
Gene:ACER3
Gene Synonym:
Exon (2)208..318
Gene:ACER3
Gene Synonym:
Exon (3)319..442
Gene:ACER3
Gene Synonym:
Exon (4)443..495
Gene:ACER3
Gene Synonym:
Exon (5)496..577
Gene:ACER3
Gene Synonym:
Exon (6)578..613
Gene:ACER3
Gene Synonym:
Exon (7)614..672
Gene:ACER3
Gene Synonym:
Exon (8)673..774
Gene:ACER3
Gene Synonym:
Exon (9)775..879
Gene:ACER3
Gene Synonym:
Exon (10)880..925
Gene:ACER3
Gene Synonym:
Exon (11)926..7449
Gene:ACER3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001300954
Organism Homo sapiens (human)
Definition Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001300954

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGATATACA GCTGTTGCAT ATTTGTGTAC TGCATGTTTG AATGTTTCAA GATCAAGAAC 
TCAGTAAACT ACCATCTGCT TTTTACCTTA GTTCTATTCA GTTTAATAGT AACCACAGTT
TACCTTAAGG TAAAAGAGCC GATATTCCAT CAGGTCATGT ATGGAATGTT GGTCTTTACA
TTAGTACTTC GATCTATTTA TATTGTTACA TGGGTTTATC CATGGCTTAG AGGACTGGGT
TATACATCAT TGGGTATATT TTTATTGGGA TTTTTATTTT GGAATATAGA TAACATATTT
TGTGAGTCAC TGAGGAACTT TCGAAAGAAG GTACCACCTA TCATAGGTAT TACCACACAA
TTTCATGCAT GGTGGCATAT TTTAACTGGC CTTGGTTCCT ATCTTCACAT CCTTTTCAGT
TTGTATACAA GAACACTTTA CCTGAGATAT AGGCCAAAAG TGAAGTTTCT CTTTGGAATC
TGGCCAGTGA TCCTGTTTGA GCCTCTCAGG AAGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45422
Clone ID Related Accession (Same CDS sequence) NM_001300954 , NM_001300955
Accession Version NM_001300955.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform c
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG702017.1, AK316120.1, AP000752.4 and AI885537.1. On Jul 17, 2014 this sequence version replaced gi:530396973. Transcript Variant: This variant (4) lacks an alternate exon and uses a downstream translation start codon compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Variants 3 and 4 both encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK316120.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGATATACA GCTGTTGCAT ATTTGTGTAC TGCATGTTTG AATGTTTCAA GATCAAGAAC 
TCAGTAAACT ACCATCTGCT TTTTACCTTA GTTCTATTCA GTTTAATAGT AACCACAGTT
TACCTTAAGG TAAAAGAGCC GATATTCCAT CAGGTCATGT ATGGAATGTT GGTCTTTACA
TTAGTACTTC GATCTATTTA TATTGTTACA TGGGTTTATC CATGGCTTAG AGGACTGGGT
TATACATCAT TGGGTATATT TTTATTGGGA TTTTTATTTT GGAATATAGA TAACATATTT
TGTGAGTCAC TGAGGAACTT TCGAAAGAAG GTACCACCTA TCATAGGTAT TACCACACAA
TTTCATGCAT GGTGGCATAT TTTAACTGGC CTTGGTTCCT ATCTTCACAT CCTTTTCAGT
TTGTATACAA GAACACTTTA CCTGAGATAT AGGCCAAAAG TGAAGTTTCT CTTTGGAATC
TGGCCAGTGA TCCTGTTTGA GCCTCTCAGG AAGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001287884.1
CDS337..855
Misc Feature(1)307..309(+)
Misc Feature(2)337..828(+)
Exon (1)1..207
Gene:ACER3
Gene Synonym:
Exon (2)208..318
Gene:ACER3
Gene Synonym:
Exon (3)319..371
Gene:ACER3
Gene Synonym:
Exon (4)372..453
Gene:ACER3
Gene Synonym:
Exon (5)454..489
Gene:ACER3
Gene Synonym:
Exon (6)490..548
Gene:ACER3
Gene Synonym:
Exon (7)549..650
Gene:ACER3
Gene Synonym:
Exon (8)651..755
Gene:ACER3
Gene Synonym:
Exon (9)756..801
Gene:ACER3
Gene Synonym:
Exon (10)802..7325
Gene:ACER3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001300955
Organism Homo sapiens (human)
Definition Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001300955

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGATATACA GCTGTTGCAT ATTTGTGTAC TGCATGTTTG AATGTTTCAA GATCAAGAAC 
TCAGTAAACT ACCATCTGCT TTTTACCTTA GTTCTATTCA GTTTAATAGT AACCACAGTT
TACCTTAAGG TAAAAGAGCC GATATTCCAT CAGGTCATGT ATGGAATGTT GGTCTTTACA
TTAGTACTTC GATCTATTTA TATTGTTACA TGGGTTTATC CATGGCTTAG AGGACTGGGT
TATACATCAT TGGGTATATT TTTATTGGGA TTTTTATTTT GGAATATAGA TAACATATTT
TGTGAGTCAC TGAGGAACTT TCGAAAGAAG GTACCACCTA TCATAGGTAT TACCACACAA
TTTCATGCAT GGTGGCATAT TTTAACTGGC CTTGGTTCCT ATCTTCACAT CCTTTTCAGT
TTGTATACAA GAACACTTTA CCTGAGATAT AGGCCAAAAG TGAAGTTTCT CTTTGGAATC
TGGCCAGTGA TCCTGTTTGA GCCTCTCAGG AAGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69362
Clone ID Related Accession (Same CDS sequence) NM_001300953
Accession Version NM_001300953.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform b
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG702017.1, AK294978.1, AP002498.3, AP000752.4 and AI885537.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK294978.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTTGGTAGG AATGGGATCC
TGGTGCTTCC ACATGACTCT GAAATATGAA ATGCAGCTAT TGGATGAACT CCCAATGATA
TACAGCTGTT GCATATTTGT GTACTGCATG TTTGAATGTT TCAAGATCAA GAACTCAGTA
AACTACCATC TGCTTTTTAC CTTAGTTCTA TTCAGTTTAA TAGTAACCAC AGTTTACCTT
AAGGTAAAAG AGCCGATATT CCATCAGGTC ATGTATGGAA TGTTGGTCTT TACATTAGTA
CTTCGATCTA TTTATATTGT TACATGGGTT TATCCATGGC TTAGAGGACT GGGTTATACA
TCATTGGGTA TATTTTTATT GGGATTTTTA TTTTGGAATA TAGATAACAT ATTTTGTGAG
TCACTGAGGA ACTTTCGAAA GAAGGTACCA CCTATCATAG GTATTACCAC ACAATTTCAT
GCATGGTGGC ATATTTTAAC TGGCCTTGGT TCCTATCTTC ACATCCTTTT CAGTTTGTAT
ACAAGAACAC TTTACCTGAG ATATAGGCCA AAAGTGAAGT TTCTCTTTGG AATCTGGCCA
GTGATCCTGT TTGAGCCTCT CAGGAAGCAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001287882.1
CDS105..797
Misc Feature(1)69..71(+)
Misc Feature(2)126..770(+)
Exon (1)1..207
Gene:ACER3
Gene Synonym:
Exon (2)208..260
Gene:ACER3
Gene Synonym:
Exon (3)261..313
Gene:ACER3
Gene Synonym:
Exon (4)314..395
Gene:ACER3
Gene Synonym:
Exon (5)396..431
Gene:ACER3
Gene Synonym:
Exon (6)432..490
Gene:ACER3
Gene Synonym:
Exon (7)491..592
Gene:ACER3
Gene Synonym:
Exon (8)593..697
Gene:ACER3
Gene Synonym:
Exon (9)698..743
Gene:ACER3
Gene Synonym:
Exon (10)744..7267
Gene:ACER3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001300953
Organism Homo sapiens (human)
Definition Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001300953

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTTGGTAGG AATGGGATCC
TGGTGCTTCC ACATGACTCT GAAATATGAA ATGCAGCTAT TGGATGAACT CCCAATGATA
TACAGCTGTT GCATATTTGT GTACTGCATG TTTGAATGTT TCAAGATCAA GAACTCAGTA
AACTACCATC TGCTTTTTAC CTTAGTTCTA TTCAGTTTAA TAGTAACCAC AGTTTACCTT
AAGGTAAAAG AGCCGATATT CCATCAGGTC ATGTATGGAA TGTTGGTCTT TACATTAGTA
CTTCGATCTA TTTATATTGT TACATGGGTT TATCCATGGC TTAGAGGACT GGGTTATACA
TCATTGGGTA TATTTTTATT GGGATTTTTA TTTTGGAATA TAGATAACAT ATTTTGTGAG
TCACTGAGGA ACTTTCGAAA GAAGGTACCA CCTATCATAG GTATTACCAC ACAATTTCAT
GCATGGTGGC ATATTTTAAC TGGCCTTGGT TCCTATCTTC ACATCCTTTT CAGTTTGTAT
ACAAGAACAC TTTACCTGAG ATATAGGCCA AAAGTGAAGT TTCTCTTTGG AATCTGGCCA
GTGATCCTGT TTGAGCCTCT CAGGAAGCAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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