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MAPK8 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAPK8
Entrez Gene ID 5599
Full Name mitogen-activated protein kinase 8
Synonyms JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c
General protein information
Preferred Names
mitogen-activated protein kinase 8
Names
mitogen-activated protein kinase 8
MAP kinase 8
JUN N-terminal kinase
c-Jun N-terminal kinase 1
stress-activated protein kinase 1
stress-activated protein kinase 1c
mitogen-activated protein kinase 8 isoform JNK1 beta2
mitogen-activated protein kinase 8 isoform JNK1 alpha1
NP_002741.1
NP_620634.1
NP_620635.1
NP_620637.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q11.22

Summary The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]. lac of sum
Disorder MIM:

601158


mRNA and Protein(s)

mRNA Protein Name
XM_011539953 XP_011538255 mitogen-activated protein kinase 8 isoform X4
XM_011539954 XP_011538256 mitogen-activated protein kinase 8 isoform X4
XM_011539955 XP_011538257 mitogen-activated protein kinase 8 isoform X4
XM_011539956 XP_011538258 mitogen-activated protein kinase 8 isoform X3
XM_011539957 XP_011538259 mitogen-activated protein kinase 8 isoform X2
XM_011539958 XP_011538260 mitogen-activated protein kinase 8 isoform X1
NM_002750 NP_002741 mitogen-activated protein kinase 8 isoform alpha1
NM_139046 NP_620634 mitogen-activated protein kinase 8 isoform beta1
NM_139049 NP_620637 mitogen-activated protein kinase 8 isoform alpha2
NM_001278547 NP_001265476 mitogen-activated protein kinase 8 JNK1 beta2
NM_001278548 NP_001265477 mitogen-activated protein kinase 8 isoform 5



Homo sapiens (human) MAPK8 NP_001265476.1
Pan troglodytes (chimpanzee) MAPK8 XP_001136927.1
Macaca mulatta (Rhesus monkey) MAPK8 XP_001108815.1
Canis lupus familiaris (dog) MAPK8 XP_005637524.1
Bos taurus (cattle) MAPK8 NP_001179903.1
Mus musculus (house mouse) Mapk8 NP_057909.1
Rattus norvegicus (Norway rat) Mapk8 NP_446281.1
Gallus gallus (chicken) MAPK8 XP_001233169.1
Danio rerio (zebrafish) mapk8a NP_001103859.1
Drosophila melanogaster (fruit fly) bsk NP_001162930.1
Caenorhabditis elegans jnk-1 NP_001021270.1
Xenopus (Silurana) tropicalis (western clawed frog) mapk8 NP_001123415.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAPK8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAPK8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22227
XM_011539953 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22227
XM_011539954 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22227
XM_011539955 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22182 XM_011539956 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu23194 XM_011539957 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu22909
XM_011539958 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22909
NM_002750 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu23194 NM_139046 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-b1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu22182 NM_139049 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu22227
NM_001278547 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-b2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22495 NM_001278548 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22227
Clone ID Related Accession (Same CDS sequence) NM_001278547 , XM_011539953 , XM_011539954 , XM_011539955
Accession Version XM_011539953.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538255.1
CDS458..1741
Misc Feature(1)530..1537(+)
Misc Feature(2)533..1420(+)
Misc Feature(3)551..1138(+)
Misc Feature(4)551..961(+)
Misc Feature(5)671..1138(+)
Misc Feature(6)791..1444(+)
Misc Feature(7)959..1027(+)
Translation

Target ORF information:

RefSeq Version XM_011539953
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539953

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22227
Clone ID Related Accession (Same CDS sequence) NM_001278547 , XM_011539953 , XM_011539954 , XM_011539955
Accession Version XM_011539954.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538256.1
CDS405..1688
Misc Feature(1)477..1484(+)
Misc Feature(2)480..1367(+)
Misc Feature(3)498..1085(+)
Misc Feature(4)498..908(+)
Misc Feature(5)618..1085(+)
Misc Feature(6)738..1391(+)
Misc Feature(7)906..974(+)
Translation

Target ORF information:

RefSeq Version XM_011539954
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539954

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22227
Clone ID Related Accession (Same CDS sequence) NM_001278547 , XM_011539953 , XM_011539954 , XM_011539955
Accession Version XM_011539955.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538257.1
CDS260..1543
Misc Feature(1)332..1339(+)
Misc Feature(2)335..1222(+)
Misc Feature(3)353..940(+)
Misc Feature(4)353..763(+)
Misc Feature(5)473..940(+)
Misc Feature(6)593..1246(+)
Misc Feature(7)761..829(+)
Translation

Target ORF information:

RefSeq Version XM_011539955
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539955

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22182
Clone ID Related Accession (Same CDS sequence) NM_139049 , XM_011539956
Accession Version XM_011539956.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC
CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538258.1
CDS458..1741
Misc Feature(1)530..1537(+)
Misc Feature(2)533..1420(+)
Misc Feature(3)551..1138(+)
Misc Feature(4)551..961(+)
Misc Feature(5)671..1138(+)
Misc Feature(6)791..1444(+)
Misc Feature(7)959..1027(+)
Translation

Target ORF information:

RefSeq Version XM_011539956
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539956

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC
CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23194
Clone ID Related Accession (Same CDS sequence) NM_139046 , XM_011539957
Accession Version XM_011539957.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1155bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGCA
CAGGTGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538259.1
CDS458..1612
Misc Feature(1)530..1537(+)
Misc Feature(2)533..1420(+)
Misc Feature(3)551..1138(+)
Misc Feature(4)551..961(+)
Misc Feature(5)671..1138(+)
Misc Feature(6)791..1444(+)
Misc Feature(7)959..1027(+)
Translation

Target ORF information:

RefSeq Version XM_011539957
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539957

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGCA
CAGGTGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22909
Clone ID Related Accession (Same CDS sequence) NM_002750 , XM_011539958
Accession Version XM_011539958.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1155bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC
CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGCA
CAGGTGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538260.1
CDS458..1612
Misc Feature(1)530..1537(+)
Misc Feature(2)533..1420(+)
Misc Feature(3)551..1138(+)
Misc Feature(4)551..961(+)
Misc Feature(5)671..1138(+)
Misc Feature(6)791..1444(+)
Misc Feature(7)959..1027(+)
Translation

Target ORF information:

RefSeq Version XM_011539958
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539958

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC
CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGCA
CAGGTGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22909
Clone ID Related Accession (Same CDS sequence) NM_002750 , XM_011539958
Accession Version NM_002750.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1155bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform alpha1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292523.1, L26318.1, AC016397.6 and AA808177.1. This sequence is a reference standard in the RefSeqGene project. On Jun 20, 2013 this sequence version replaced gi:20986493. Transcript Variant: This variant (JNK1-a1) uses a different acceptor splice site in the last coding exon compared to transcript variant JNK1-a2, resulting in a frameshift and a shorter isoform (JNK1 alpha1) with a different C-terminus, compared to isoform JNK1 alpha2. The JNK1-a1 variant differs from the JNK1-b1 variant in the use of an alternate internal coding exon of the same length. Thus, JNK1 alpha1 isoform is the same length as JNK1 beta1 isoform, with a few aa difference in an internal protein segment. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L26318.1, AB451231.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC
CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGCA
CAGGTGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002741.1
CDS50..1204
Misc Feature(1)35..37(+)
Misc Feature(2)122..1129(+)
Misc Feature(3)125..1012(+)
Misc Feature(4)143..730(+)
Misc Feature(5)143..553(+)
Misc Feature(6)263..730(+)
Misc Feature(7)383..1036(+)
Misc Feature(8)551..619(+)
Misc Feature(9)596..604(+)
Misc Feature(10)596..598(+)
Misc Feature(11)596..598(+)
Misc Feature(12)602..604(+)
Misc Feature(13)602..604(+)
Misc Feature(14)602..604(+)
Exon (1)1..171
Gene:MAPK8
Gene Synonym:
Exon (2)172..301
Gene:MAPK8
Gene Synonym:
Exon (3)302..360
Gene:MAPK8
Gene Synonym:
Exon (4)361..499
Gene:MAPK8
Gene Synonym:
Exon (5)500..665
Gene:MAPK8
Gene Synonym:
Exon (6)666..737
Gene:MAPK8
Gene Synonym:
Exon (7)738..920
Gene:MAPK8
Gene Synonym:
Exon (8)921..1045
Gene:MAPK8
Gene Synonym:
Exon (9)1046..1109
Gene:MAPK8
Gene Synonym:
Exon (10)1110..1187
Gene:MAPK8
Gene Synonym:
Exon (11)1188..5669
Gene:MAPK8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002750
Organism Homo sapiens (human)
Definition Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002750

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC
CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGCA
CAGGTGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23194
Clone ID Related Accession (Same CDS sequence) NM_139046 , XM_011539957
Accession Version NM_139046.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1155bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform beta1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292523.1, U35004.1, AC016397.6 and AA808177.1. On Jun 20, 2013 this sequence version replaced gi:20986518. Transcript Variant: This variant (JNK1-b1) uses a different acceptor splice site in the last coding exon compared to transcript variant JNK1-b2, resulting in a frameshift and a shorter isoform (JNK1 beta1) with a different C-terminus, compared to isoform JNK1 beta2. The JNK1-b1 variant differs from the JNK1-a1 variant in the use of an alternate internal coding exon of the same length. Thus, JNK1 beta1 isoform is the same length as JNK1 alpha1 isoform, with a few aa difference in an internal protein segment. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144063.1, U35004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGCA
CAGGTGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_620634.1
CDS50..1204
Misc Feature(1)35..37(+)
Misc Feature(2)122..1129(+)
Misc Feature(3)125..1012(+)
Misc Feature(4)143..730(+)
Misc Feature(5)143..553(+)
Misc Feature(6)263..730(+)
Misc Feature(7)383..1036(+)
Misc Feature(8)551..619(+)
Misc Feature(9)596..598(+)
Misc Feature(10)602..604(+)
Misc Feature(11)602..604(+)
Exon (1)1..171
Gene:MAPK8
Gene Synonym:
Exon (2)172..301
Gene:MAPK8
Gene Synonym:
Exon (3)302..360
Gene:MAPK8
Gene Synonym:
Exon (4)361..499
Gene:MAPK8
Gene Synonym:
Exon (5)500..665
Gene:MAPK8
Gene Synonym:
Exon (6)666..737
Gene:MAPK8
Gene Synonym:
Exon (7)738..920
Gene:MAPK8
Gene Synonym:
Exon (8)921..1045
Gene:MAPK8
Gene Synonym:
Exon (9)1046..1109
Gene:MAPK8
Gene Synonym:
Exon (10)1110..1187
Gene:MAPK8
Gene Synonym:
Exon (11)1188..5669
Gene:MAPK8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_139046
Organism Homo sapiens (human)
Definition Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-b1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_139046

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGCA
CAGGTGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22182
Clone ID Related Accession (Same CDS sequence) NM_139049 , XM_011539956
Accession Version NM_139049.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform alpha2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292523.1, U34822.1, AC016397.6 and AA808177.1. On Jun 20, 2013 this sequence version replaced gi:20986522. Transcript Variant: This variant (JNK1-a2) encodes the longer of the two JNK1 alpha isoforms (JNK1 alpha2). The JNK1-a2 variant differs from the JNK1-b2 variant in the use of an alternate internal coding exon of the same length. Thus, JNK1 alpha2 isoform is the same length as JNK1 beta2 isoform, with a few aa difference in an internal protein segment. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U34822.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC
CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_620637.1
CDS50..1333
Misc Feature(1)35..37(+)
Misc Feature(2)122..1129(+)
Misc Feature(3)125..1012(+)
Misc Feature(4)143..730(+)
Misc Feature(5)143..553(+)
Misc Feature(6)263..730(+)
Misc Feature(7)383..1036(+)
Misc Feature(8)551..619(+)
Misc Feature(9)596..604(+)
Misc Feature(10)596..598(+)
Misc Feature(11)596..598(+)
Misc Feature(12)602..604(+)
Misc Feature(13)602..604(+)
Misc Feature(14)602..604(+)
Misc Feature(15)1178..1180(+)
Exon (1)1..171
Gene:MAPK8
Gene Synonym:
Exon (2)172..301
Gene:MAPK8
Gene Synonym:
Exon (3)302..360
Gene:MAPK8
Gene Synonym:
Exon (4)361..499
Gene:MAPK8
Gene Synonym:
Exon (5)500..665
Gene:MAPK8
Gene Synonym:
Exon (6)666..737
Gene:MAPK8
Gene Synonym:
Exon (7)738..920
Gene:MAPK8
Gene Synonym:
Exon (8)921..1045
Gene:MAPK8
Gene Synonym:
Exon (9)1046..1109
Gene:MAPK8
Gene Synonym:
Exon (10)1110..1187
Gene:MAPK8
Gene Synonym:
Exon (11)1188..5664
Gene:MAPK8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_139049
Organism Homo sapiens (human)
Definition Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_139049

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC
CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22227
Clone ID Related Accession (Same CDS sequence) NM_001278547 , XM_011539953 , XM_011539954 , XM_011539955
Accession Version NM_001278547.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 JNK1 beta2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA159335.1, AK292523.1, AC016397.6 and AA808177.1. On Jun 20, 2013 this sequence version replaced gi:20986520. Transcript Variant: This variant (JNK1-b2) encodes the longer of the two JNK1 beta isoforms (JNK1 beta2). The JNK1-b2 variant differs from the JNK1-a2 variant in the use of an alternate internal coding exon of the same length. Thus, JNK1 beta2 isoform is the same length as JNK1 alpha2 isoform, with a few aa difference in an internal protein segment. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292523.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001265476.1
CDS233..1516
Misc Feature(1)218..220(+)
Misc Feature(2)305..1312(+)
Misc Feature(3)308..1195(+)
Misc Feature(4)326..913(+)
Misc Feature(5)326..736(+)
Misc Feature(6)446..913(+)
Misc Feature(7)566..1219(+)
Misc Feature(8)734..802(+)
Misc Feature(9)779..787(+)
Misc Feature(10)779..781(+)
Misc Feature(11)785..787(+)
Exon (1)1..183
Gene:MAPK8
Gene Synonym:
Exon (2)184..354
Gene:MAPK8
Gene Synonym:
Exon (3)355..484
Gene:MAPK8
Gene Synonym:
Exon (4)485..543
Gene:MAPK8
Gene Synonym:
Exon (5)544..682
Gene:MAPK8
Gene Synonym:
Exon (6)683..848
Gene:MAPK8
Gene Synonym:
Exon (7)849..920
Gene:MAPK8
Gene Synonym:
Exon (8)921..1103
Gene:MAPK8
Gene Synonym:
Exon (9)1104..1228
Gene:MAPK8
Gene Synonym:
Exon (10)1229..1292
Gene:MAPK8
Gene Synonym:
Exon (11)1293..1370
Gene:MAPK8
Gene Synonym:
Exon (12)1371..5847
Gene:MAPK8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001278547
Organism Homo sapiens (human)
Definition Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-b2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001278547

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22495
Clone ID Related Accession (Same CDS sequence) NM_001278548
Accession Version NM_001278548.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 927bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292523.1, DQ234352.1, AC016397.6 and AA808177.1. Transcript Variant: This variant (5) lacks an alternate in-frame segment and uses a different acceptor splice site in the last coding exon compared to transcript variant JNK1-b2, resulting in a frameshift and a shorter isoform (5) with a different C-terminus compared to isoform JNK1 beta2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ234352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGCTGA CTCAGAACAC AACAAACTTA AAGCCAGTCA GGCAAGGGAT
TTGTTATCCA AAATGCTGGT AATAGATGCA TCTAAAAGGA TCTCTGTAGA TGAAGCTCTC
CAACACCCGT ACATCAATGT CTGGTATGAT CCTTCTGAAG CAGAAGCTCC ACCACCAAAG
ATCCCTGACA AGCAGTTAGA TGAAAGGGAA CACACAATAG AAGAGTGGAA AGAATTGATA
TATAAGGAAG TTATGGACTT GGAGGAGAGA ACCAAGAATG GAGTTATACG GGGGCAGCCC
TCTCCTTTAG CACAGGTGCA GCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001265477.1
CDS50..976
Misc Feature(1)35..37(+)
Misc Feature(2)122..901(+)
Misc Feature(3)125..784(+)
Misc Feature(4)143..556(+)
Misc Feature(5)596..604(+)
Misc Feature(6)596..598(+)
Misc Feature(7)602..604(+)
Exon (1)1..171
Gene:MAPK8
Gene Synonym:
Exon (2)172..301
Gene:MAPK8
Gene Synonym:
Exon (3)302..360
Gene:MAPK8
Gene Synonym:
Exon (4)361..499
Gene:MAPK8
Gene Synonym:
Exon (5)500..665
Gene:MAPK8
Gene Synonym:
Exon (6)666..692
Gene:MAPK8
Gene Synonym:
Exon (7)693..817
Gene:MAPK8
Gene Synonym:
Exon (8)818..881
Gene:MAPK8
Gene Synonym:
Exon (9)882..959
Gene:MAPK8
Gene Synonym:
Exon (10)960..5441
Gene:MAPK8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001278548
Organism Homo sapiens (human)
Definition Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001278548

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGCTGA CTCAGAACAC AACAAACTTA AAGCCAGTCA GGCAAGGGAT
TTGTTATCCA AAATGCTGGT AATAGATGCA TCTAAAAGGA TCTCTGTAGA TGAAGCTCTC
CAACACCCGT ACATCAATGT CTGGTATGAT CCTTCTGAAG CAGAAGCTCC ACCACCAAAG
ATCCCTGACA AGCAGTTAGA TGAAAGGGAA CACACAATAG AAGAGTGGAA AGAATTGATA
TATAAGGAAG TTATGGACTT GGAGGAGAGA ACCAAGAATG GAGTTATACG GGGGCAGCCC
TCTCCTTTAG CACAGGTGCA GCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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