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PRPS1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PRPS1
Entrez Gene ID 5631
Full Name phosphoribosyl pyrophosphate synthetase 1
Synonyms ARTS,CMTX5,DFN2,DFNX1,PPRibP,PRS-I,PRSI
General protein information
Preferred Names

phosphoribosyl pyrophosphate synthetase 1

Names

ribose-phosphate pyrophosphokinase 1
dJ1070B1.2 (phosphoribosyl pyrophosphate synthetase 1)
deafness 2, perceptive, congenital
deafness, X-linked 2, perceptive, congenital
phosphoribosyl pyrophosphate synthase I
ribose-phosphate diphosphokinase 1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xq22.3

Summary This gene encodes an enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Defects in this gene are a cause of phosphoribosylpyrophosphate synthetase superactivity, Charcot-Marie-Tooth disease X-linked recessive type 5 and Arts Syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011].
Disorder MIM:

311850

Disorder Html: Gout, PRPS-related, 300661 (3); Phosphoribosylpyrophosphate

mRNA and Protein(s)

mRNA Protein Name
NM_001204402.1 NP_001191331.1 ribose-phosphate pyrophosphokinase 1 isoform 2
NM_002764.3 NP_002755.1 ribose-phosphate pyrophosphokinase 1 isoform 1



Macaca mulatta (rhesus monkey) PRPS1 XP_001095604.1
Pan troglodytes (chimpanzee) PRPS1 XP_001144504.1
Gallus gallus (chicken) PRPS1 XP_004940671.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004890 XP_001688552.1
Oryza sativa (rice) Os02g0127700 NP_001045763.1
Danio rerio (zebrafish) prps1b NP_001070036.1
Arabidopsis thaliana (thale cress) AT2G35390 NP_850244.1
Canis lupus familiaris (dog) PRPS1 XP_005641702.1
Bos taurus (cattle) PRPS1 NP_001039654.1
Rattus norvegicus (Norway rat) Prps1 NP_058939.1
Drosophila melanogaster (fruit fly) CG6767 NP_729528.2
Arabidopsis thaliana (thale cress) PRS2 NP_174516.1
Mus musculus (house mouse) Prps1 NP_067438.1
Arabidopsis thaliana (thale cress) AT2G44530 NP_181981.2
Homo sapiens (human) PRPS1 NP_002755.1


Related articles in PubMed

MicroRNA-124 reduces the pentose phosphate pathway and proliferation by targeting PRPS1 and RPIA mRNAs in human colorectal cancer cells.
Qiu Z, Guo W, Wang Q, Chen Z, Huang S, Zhao F, Yao M, Zhao Y, He X
Gastroenterology149(6)1587-1598.e11(2015 Nov)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High PRPS1 expression is associated with chemoresistance in breast cancer.
Title: PRPS1 silencing reverses cisplatin resistance in human breast cancer cells.

CRC cells that overexpressed miR124 or with knockdown of RPIA or PRPS1 had reduced DNA synthesis and proliferation, whereas cells incubated with an inhibitor of miR124 had significantly increased DNA synthesis and proliferation and formed more colonies.
Title: MicroRNA-124 reduces the pentose phosphate pathway and proliferation by targeting PRPS1 and RPIA mRNAs in human colorectal cancer cells.

the de novo purine synthesis inhibitor lometrexol effectively abrogated PRPS1 mutant-driven drug resistance.
Title: Negative feedback-defective PRPS1 mutants drive thiopurine resistance in relapsed childhood ALL.

evaluation of current literature on PRPS1-related syndromes and summaries of potential therapies [review]
Title: Association of PRPS1 Mutations with Disease Phenotypes.

The expression of different genes encoding subunits of PRPS enzyme is affected by hypoxia in tumor glioma cells, but the effect of hypoxia is modified by suppression of endoplasmic reticulum stress signaling enzyme ERN1.
Title: Expression of phosphoribosyl pyrophosphate synthetase genes in U87 glioma cells with ERN1 knockdown: effect of hypoxia and endoplasmic reticulum stress.

The following PRPS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRPS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu21900 NM_001204402.1
Latest version!
Homo sapiens phosphoribosyl pyrophosphate synthetase 1 (PRPS1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26049 NM_002764.3
Latest version!
Homo sapiens phosphoribosyl pyrophosphate synthetase 1 (PRPS1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

ORF Online Only Promotion

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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu21900
Clone ID Related Accession (Same CDS sequence) NM_001204402.1
Accession Version NM_001204402.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 345bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-09-16
Organism Homo sapiens(Human)
Product ribose-phosphate pyrophosphokinase 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137787.11, DC411129.1, AK316467.1 and D00860.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGTGCTTG TGGGAGATGT GAAGGATCGG GTGGCCATCC TTGTGGATGA CATGGCTGAC 
ACTTGTGGCA CAATCTGCCA TGCAGCTGAC AAACTTCTCT CAGCTGGCGC CACCAGAGTT
TATGCCATCT TGACTCATGG AATCTTCTCC GGTCCTGCTA TTTCTCGCAT CAACAACGCA
TGCTTTGAGG CAGTAGTAGT CACCAATACC ATACCTCAGG AGGACAAGAT GAAGCATTGC
TCCAAAATAC AGGTGATTGA CATCTCTATG ATCCTTGCAG AAGCCATCAG GAGAACTCAC
AATGGAGAAT CCGTTTCTTA CCTATTCAGC CATGTCCCTT TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191331.1
CDS410..754
Misc Feature(1)326..328
Misc Feature(2)<410..736
Misc Feature(3)order(455..463,467..481,551..553)
Exon (1)1..327
Exon (2)328..501
Exon (3)502..661
Exon (4)662..1748
Translation

Target ORF information:

RefSeq Version NM_001204402.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphoribosyl pyrophosphate synthetase 1 (PRPS1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204402.1

ORF Insert Sequence:

1
61
121
181
241
301
ATGGTGCTTG TGGGAGATGT GAAGGATCGG GTGGCCATCC TTGTGGATGA CATGGCTGAC 
ACTTGTGGCA CAATCTGCCA TGCAGCTGAC AAACTTCTCT CAGCTGGCGC CACCAGAGTT
TATGCCATCT TGACTCATGG AATCTTCTCC GGTCCTGCTA TTTCTCGCAT CAACAACGCA
TGCTTTGAGG CAGTAGTAGT CACCAATACC ATACCTCAGG AGGACAAGAT GAAGCATTGC
TCCAAAATAC AGGTGATTGA CATCTCTATG ATCCTTGCAG AAGCCATCAG GAGAACTCAC
AATGGAGAAT CCGTTTCTTA CCTATTCAGC CATGTCCCTT TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26049
Clone ID Related Accession (Same CDS sequence) NM_002764.3
Accession Version NM_002764.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 957bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-02
Organism Homo sapiens(Human)
Product ribose-phosphate pyrophosphokinase 1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137787.11 and BC001605.1. This sequence is a reference standard in the RefSeqGene project. On Jan 12, 2008 this sequence version replaced NM_002764.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.136208.1, SRR1163658.177459.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCGAATA TCAAAATCTT CAGCGGCAGC TCCCACCAGG ACTTATCTCA GAAAATTGCT 
GACCGCCTGG GCCTGGAGCT AGGCAAGGTG GTGACTAAGA AATTCAGCAA CCAGGAGACC
TGTGTGGAAA TTGGTGAAAG TGTACGTGGA GAGGATGTCT ACATTGTTCA GAGTGGTTGT
GGCGAAATCA ATGACAATTT AATGGAGCTT TTGATCATGA TTAATGCCTG CAAGATTGCT
TCAGCCAGCC GGGTTACTGC AGTCATCCCA TGCTTCCCTT ATGCCCGGCA GGATAAGAAA
GATAAGAGCC GGGCGCCAAT CTCAGCCAAG CTTGTTGCAA ATATGCTATC TGTAGCAGGT
GCAGATCATA TTATCACCAT GGACCTACAT GCTTCTCAAA TTCAGGGCTT TTTTGATATC
CCAGTAGACA ATTTGTATGC AGAGCCGGCT GTCCTAAAGT GGATAAGGGA GAATATCTCT
GAGTGGAGGA ACTGCACTAT TGTCTCACCT GATGCTGGTG GAGCTAAGAG AGTGACCTCC
ATTGCAGACA GGCTGAATGT GGACTTTGCC TTGATTCACA AAGAACGGAA GAAGGCCAAT
GAAGTGGACC GCATGGTGCT TGTGGGAGAT GTGAAGGATC GGGTGGCCAT CCTTGTGGAT
GACATGGCTG ACACTTGTGG CACAATCTGC CATGCAGCTG ACAAACTTCT CTCAGCTGGC
GCCACCAGAG TTTATGCCAT CTTGACTCAT GGAATCTTCT CCGGTCCTGC TATTTCTCGC
ATCAACAACG CATGCTTTGA GGCAGTAGTA GTCACCAATA CCATACCTCA GGAGGACAAG
ATGAAGCATT GCTCCAAAAT ACAGGTGATT GACATCTCTA TGATCCTTGC AGAAGCCATC
AGGAGAACTC ACAATGGAGA ATCCGTTTCT TACCTATTCA GCCATGTCCC TTTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002755.1
CDS206..1162
Misc Feature(1)194..196
Misc Feature(2)206..1144
Misc Feature(3)215..565
Misc Feature(4)686..1144
Misc Feature(5)order(716..718,722..724,863..871,875..889,959..961)
Misc Feature(6)839..886
Exon (1)1..327
Exon (2)328..511
Exon (3)512..610
Exon (4)611..735
Exon (5)736..909
Exon (6)910..1069
Exon (7)1070..2156
Translation

Target ORF information:

RefSeq Version NM_002764.3
Organism Homo sapiens(Human)
Definition Homo sapiens phosphoribosyl pyrophosphate synthetase 1 (PRPS1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002764.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCGAATA TCAAAATCTT CAGCGGCAGC TCCCACCAGG ACTTATCTCA GAAAATTGCT 
GACCGCCTGG GCCTGGAGCT AGGCAAGGTG GTGACTAAGA AATTCAGCAA CCAGGAGACC
TGTGTGGAAA TTGGTGAAAG TGTACGTGGA GAGGATGTCT ACATTGTTCA GAGTGGTTGT
GGCGAAATCA ATGACAATTT AATGGAGCTT TTGATCATGA TTAATGCCTG CAAGATTGCT
TCAGCCAGCC GGGTTACTGC AGTCATCCCA TGCTTCCCTT ATGCCCGGCA GGATAAGAAA
GATAAGAGCC GGGCGCCAAT CTCAGCCAAG CTTGTTGCAA ATATGCTATC TGTAGCAGGT
GCAGATCATA TTATCACCAT GGACCTACAT GCTTCTCAAA TTCAGGGCTT TTTTGATATC
CCAGTAGACA ATTTGTATGC AGAGCCGGCT GTCCTAAAGT GGATAAGGGA GAATATCTCT
GAGTGGAGGA ACTGCACTAT TGTCTCACCT GATGCTGGTG GAGCTAAGAG AGTGACCTCC
ATTGCAGACA GGCTGAATGT GGACTTTGCC TTGATTCACA AAGAACGGAA GAAGGCCAAT
GAAGTGGACC GCATGGTGCT TGTGGGAGAT GTGAAGGATC GGGTGGCCAT CCTTGTGGAT
GACATGGCTG ACACTTGTGG CACAATCTGC CATGCAGCTG ACAAACTTCT CTCAGCTGGC
GCCACCAGAG TTTATGCCAT CTTGACTCAT GGAATCTTCT CCGGTCCTGC TATTTCTCGC
ATCAACAACG CATGCTTTGA GGCAGTAGTA GTCACCAATA CCATACCTCA GGAGGACAAG
ATGAAGCATT GCTCCAAAAT ACAGGTGATT GACATCTCTA TGATCCTTGC AGAAGCCATC
AGGAGAACTC ACAATGGAGA ATCCGTTTCT TACCTATTCA GCCATGTCCC TTTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MicroRNA-124 reduces the pentose phosphate pathway and proliferation by targeting PRPS1 and RPIA mRNAs in human colorectal cancer cells.
Gastroenterology149(6)1587-1598.e11(2015 Nov)
Qiu Z,Guo W,Wang Q,Chen Z,Huang S,Zhao F,Yao M,Zhao Y,He X


book

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M


 
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