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INPP5E cDNA ORF clone, Homo sapiens(Human)

Gene Symbol INPP5E
Entrez Gene ID 56623
Full Name inositol polyphosphate-5-phosphatase E
Synonyms CORS1,CPD4,JBTS1,MORMS,PPI5PIV,pharbin
General protein information
Preferred Names

inositol polyphosphate-5-phosphatase E

Names

72 kDa inositol polyphosphate 5-phosphatase
phosphatidylinositol polyphosphate 5-phosphatase type IV
phosphatidylinositol-4,5-bisphosphate 5-phosphatase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

9

9q34.3

Disorder MIM:

613037

Disorder Html: Mental retardation, truncal obesity, retinal dystrophy, and

mRNA and Protein(s)

mRNA Protein Name
NM_019892.5 NP_063945.2 72 kDa inositol polyphosphate 5-phosphatase isoform 1
XM_017014926.1 XP_016870415.1 72 kDa inositol polyphosphate 5-phosphatase isoform X1
NM_001318502.1 NP_001305431.1 72 kDa inositol polyphosphate 5-phosphatase isoform 2



Macaca mulatta (rhesus monkey) INPP5E XP_001095203.1
Mus musculus (house mouse) Inpp5e NP_149125.1
Xenopus tropicalis (tropical clawed frog) inpp5e XP_002935265.1
Pan troglodytes (chimpanzee) INPP5E NP_001070981.1
Rattus norvegicus (Norway rat) Inpp5e NP_446084.1
Homo sapiens (human) INPP5E NP_063945.2
Canis lupus familiaris (dog) INPP5E XP_005625166.1
Bos taurus (cattle) INPP5E NP_001179019.1
Gallus gallus (chicken) INPP5E XP_415418.2
Danio rerio (zebrafish) inpp5e NP_001096089.2


Related articles in PubMed

Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.
Xu Q, Zhang Y, Wei Q, Huang Y, Hu J, Ling K
Nature communications710777(2016 Feb)

INPP5E interacts with AURKA, linking phosphoinositide signaling to primary cilium stability.
Plotnikova OV, Seo S, Cottle DL, Conduit S, Hakim S, Dyson JM, Mitchell CA, Smyth IM
Journal of cell science128(2)364-72(2015 Jan)

A homozygous PDE6D mutation in Joubert syndrome impairs targeting of farnesylated INPP5E protein to the primary cilium.
Thomas S, Wright KJ, Le Corre S, Micalizzi A, Romani M, Abhyankar A, Saada J, Perrault I, Amiel J, Litzler J, Filhol E, Elkhartoufi N, Kwong M, Casanova JL, Boddaert N, Baehr W, Lyonnet S, Munnich A, Burglen L, Chassaing N, Encha-Ravazi F, Vekemans M, Gleeson JG, Valente EM, Jackson PK, Drummond IA, Saunier S, Attié-Bitach T
Human mutation35(1)137-46(2014 Jan)

Phenotypic spectrum and prevalence of INPP5E mutations in Joubert syndrome and related disorders.
Travaglini L, Brancati F, Silhavy J, Iannicelli M, Nickerson E, Elkhartoufi N, Scott E, Spencer E, Gabriel S, Thomas S, Ben-Zeev B, Bertini E, Boltshauser E, Chaouch M, Cilio MR, de Jong MM, Kayserili H, Ogur G, Poretti A, Signorini S, Uziel G, Zaki MS, , Johnson C, Attié-Bitach T, Gleeson JG, Valente EM
European journal of human genetics : EJHG21(10)1074-8(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

INPP5E localizes to centrosomes, chromosomes, and kinetochores in early mitosis and shuttles to the midzone spindle at mitotic exit.
Title: INPP5E Preserves Genomic Stability through Regulation of Mitosis.

ARL13B regulates IFT-A-mediated retrograde protein trafficking within cilia through its interaction with INPP5E.
Title: Regulation of ciliary retrograde protein trafficking by the Joubert syndrome proteins ARL13B and INPP5E.

INPP5E is an essential point of convergence between Hedgehog and phosphoinositide signaling at cilia that maintains transition zone function and Hedgehog-dependent embryonic development.
Title: INPP5E regulates phosphoinositide-dependent cilia transition zone function.

In neuronal cells, INPP5E knockdown strongly inhibited autophagy by impairing the autophagosome-lysosome fusion step.
Title: Autophagosome-lysosome fusion in neurons requires INPP5E, a protein associated with Joubert syndrome.

PIPKIgamma and INPP5E localize to the centrosome and coordinate the initiation of ciliogenesis.
Title: Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.

The following INPP5E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the INPP5E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu98757 XM_017014926.1
Latest version!
Homo sapiens inositol polyphosphate-5-phosphatase E (INPP5E), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu27383 NM_019892.5
Latest version!
Homo sapiens inositol polyphosphate-5-phosphatase E (INPP5E), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $265.30
$379.00
OHu43395 NM_001318502.1
Latest version!
Homo sapiens inositol polyphosphate-5-phosphatase E (INPP5E), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu98757
Clone ID Related Accession (Same CDS sequence) XM_017014926.1
Accession Version XM_017014926.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1809bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 72 kDa inositol polyphosphate 5-phosphatase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCCGTCCA AGGCGGAGAA TCTGCGGCCC TCCGAGCCGG CCCCGCAGCC GCCGGAAGGG 
AGGACGCTCC AAGGACAGCT TCCCGGCGCT CCGCCGGCCC AGCGCGCGGG GTCCCCACCC
GATGCTCCGG GCTCCGAGAG CCCCGCGCTT GCCTGCAGCA CTCCGGCCAC GCCCAGCGGC
GAGGACCCGC CAGCCCGAGC AGCACCCATC GCCCCGCGGC CCCCCGCCAG GCCTCGACTG
GAGCGAGCCC TGTCCCTGGA CGACAAGGGC TGGAGGAGGA GGCGTTTTCG AGGCAGCCAG
GAGGACCTGG AAGCCCGGAA TGGGACCAGT CCCTCCAGGG GCTCAGTGCA GAGCGAGGGC
CCCGGGGCCC CCGCCCACAG CTGCTCCCCG CCCTGCCTGA GCACCTCCTT GCAGGAAATC
CCCAAGTCCC GCGGGGTCCT GAGCAGTGAG AGAGGGAGCC CGTCCTCGGG GGGTAACCCT
CTCTCTGGGG TGGCCAGCAG CTCCCCGAAC CTCCCGCACA GAGACGCCGC CGTGGCGGGG
AGCTCGCCCA GGCTGCCCAG CCTGCTGCCC CCGCGCCCAC CGCCTGCCCT GAGCCTGGAC
ATCGCCTCCG ACTCCCTGAG GACAGCAAAC AAGGTCGACT CGGATCTTGC AGACTACAAG
CTCCGCGCGC AGCCGCTCCT GGTGCGGGCC CACAGCAGCC TGGGCCCCGG CCGGCCGCGG
AGCCCCCTGG CCTGCGACGA CTGTTCCCTT CGCTCGGCCA AATCCTCCTT CAGCCTCCTG
GCGCCCATCC GCAGCAAGGA CGTCCGCAGC AGGAGTTACC TGGAGGGCAG CCTCCTGGCC
AGCGGGGCCC TGTTGGGGGC GGATGAGCTG GCCCGCTACT TCCCAGACCG GAACGTGGCA
CTCTTCGTGG CCACCTGGAA CATGCAGGGC CAGAAGGAGC TCCCGCCCAG CCTGGACGAG
TTCCTGCTCC CAGCCGAGGC CGACTATGCC CAGGACCTGT ATGTCATCGG GGTCCAGGAG
GGCTGTTCTG ACAGGCGGGA GTGGGAGACT CGTCTGCAGG AGACGCTGGG CCCGCACTAT
GTGCTGCTGT CCTCGGCGGC CCACGGCGTG CTCTACATGT CGCTCTTCAT CCGCAGGGAC
CTCATCTGGT TCTGCTCAGA GGTGGAGTGC TCCACGGTGA CCACACGCAT CGTGTCTCAG
ATCAAGACCA AGGGGGCCTT GGGCATCAGC TTCACCTTTT TTGGCACTTC CTTCCTCTTC
ATCACGTCCC ACTTCACCTC AGGTGACGGG AAGGTGGCGG AGCGGCTGCT GGACTACACC
AGGACTGTAC AAGCCCTGGT CCTGCCCAGA AATGTGCCCG ACACCAACCC CTATCGCTCC
AGCGCAGCGG ACGTCACCAC CCGCTTCGAT GAGGTGTTCT GGTTTGGAGA CTTCAACTTC
CGCCTGAGTG GCGGGCGCAC AGTCGTGGAC GCCCTCCTGT GCCAGGGCCT GGTGGTGGAC
GTGCCGGCGC TGCTGCAGCA CGACCAGCTC ATCCGGGAGA TGCGGAAAGG GTCCATCTTC
AAGGGCTTCC AGGAGCCGGA CATCCACTTC CTCCCATCAT ACAAGTTTGA CATCGGGAAG
GACACGTACG ACAGCACCTC CAAGCAGAGG ACGCCCTCAT ACACGGACCG CGTCTTGTAC
AGAAGCCGCC ACAAGGGTGA CATCTGTCCT GTGAGCTACT CTTCCTGCCC CGGGATCAAG
ACGTCCGACC ACCGCCCTGT GTATGGCCTC TTCCGGGTGA AAGTGAGGCC GGGGCGAGAC
AAGAATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016870415.1
CDS443..2251
Translation

Target ORF information:

RefSeq Version XM_017014926.1
Organism Homo sapiens(Human)
Definition Homo sapiens inositol polyphosphate-5-phosphatase E (INPP5E), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017014926.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCCGTCCA AGGCGGAGAA TCTGCGGCCC TCCGAGCCGG CCCCGCAGCC GCCGGAAGGG 
AGGACGCTCC AAGGACAGCT TCCCGGCGCT CCGCCGGCCC AGCGCGCGGG GTCCCCACCC
GATGCTCCGG GCTCCGAGAG CCCCGCGCTT GCCTGCAGCA CTCCGGCCAC GCCCAGCGGC
GAGGACCCGC CAGCCCGAGC AGCACCCATC GCCCCGCGGC CCCCCGCCAG GCCTCGACTG
GAGCGAGCCC TGTCCCTGGA CGACAAGGGC TGGAGGAGGA GGCGTTTTCG AGGCAGCCAG
GAGGACCTGG AAGCCCGGAA TGGGACCAGT CCCTCCAGGG GCTCAGTGCA GAGCGAGGGC
CCCGGGGCCC CCGCCCACAG CTGCTCCCCG CCCTGCCTGA GCACCTCCTT GCAGGAAATC
CCCAAGTCCC GCGGGGTCCT GAGCAGTGAG AGAGGGAGCC CGTCCTCGGG GGGTAACCCT
CTCTCTGGGG TGGCCAGCAG CTCCCCGAAC CTCCCGCACA GAGACGCCGC CGTGGCGGGG
AGCTCGCCCA GGCTGCCCAG CCTGCTGCCC CCGCGCCCAC CGCCTGCCCT GAGCCTGGAC
ATCGCCTCCG ACTCCCTGAG GACAGCAAAC AAGGTCGACT CGGATCTTGC AGACTACAAG
CTCCGCGCGC AGCCGCTCCT GGTGCGGGCC CACAGCAGCC TGGGCCCCGG CCGGCCGCGG
AGCCCCCTGG CCTGCGACGA CTGTTCCCTT CGCTCGGCCA AATCCTCCTT CAGCCTCCTG
GCGCCCATCC GCAGCAAGGA CGTCCGCAGC AGGAGTTACC TGGAGGGCAG CCTCCTGGCC
AGCGGGGCCC TGTTGGGGGC GGATGAGCTG GCCCGCTACT TCCCAGACCG GAACGTGGCA
CTCTTCGTGG CCACCTGGAA CATGCAGGGC CAGAAGGAGC TCCCGCCCAG CCTGGACGAG
TTCCTGCTCC CAGCCGAGGC CGACTATGCC CAGGACCTGT ATGTCATCGG GGTCCAGGAG
GGCTGTTCTG ACAGGCGGGA GTGGGAGACT CGTCTGCAGG AGACGCTGGG CCCGCACTAT
GTGCTGCTGT CCTCGGCGGC CCACGGCGTG CTCTACATGT CGCTCTTCAT CCGCAGGGAC
CTCATCTGGT TCTGCTCAGA GGTGGAGTGC TCCACGGTGA CCACACGCAT CGTGTCTCAG
ATCAAGACCA AGGGGGCCTT GGGCATCAGC TTCACCTTTT TTGGCACTTC CTTCCTCTTC
ATCACGTCCC ACTTCACCTC AGGTGACGGG AAGGTGGCGG AGCGGCTGCT GGACTACACC
AGGACTGTAC AAGCCCTGGT CCTGCCCAGA AATGTGCCCG ACACCAACCC CTATCGCTCC
AGCGCAGCGG ACGTCACCAC CCGCTTCGAT GAGGTGTTCT GGTTTGGAGA CTTCAACTTC
CGCCTGAGTG GCGGGCGCAC AGTCGTGGAC GCCCTCCTGT GCCAGGGCCT GGTGGTGGAC
GTGCCGGCGC TGCTGCAGCA CGACCAGCTC ATCCGGGAGA TGCGGAAAGG GTCCATCTTC
AAGGGCTTCC AGGAGCCGGA CATCCACTTC CTCCCATCAT ACAAGTTTGA CATCGGGAAG
GACACGTACG ACAGCACCTC CAAGCAGAGG ACGCCCTCAT ACACGGACCG CGTCTTGTAC
AGAAGCCGCC ACAAGGGTGA CATCTGTCCT GTGAGCTACT CTTCCTGCCC CGGGATCAAG
ACGTCCGACC ACCGCCCTGT GTATGGCCTC TTCCGGGTGA AAGTGAGGCC GGGGCGAGAC
AAGAATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27383
Clone ID Related Accession (Same CDS sequence) NM_019892.5
Accession Version NM_019892.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-13
Organism Homo sapiens(Human)
Product 72 kDa inositol polyphosphate 5-phosphatase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832846.1, BC028032.1 and BE044535.1. This sequence is a reference standard in the RefSeqGene project. On Jan 8, 2016 this sequence version replaced NM_019892.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028032.1, AF187891.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCCGTCCA AGGCGGAGAA TCTGCGGCCC TCCGAGCCGG CCCCGCAGCC GCCGGAAGGG 
AGGACGCTCC AAGGACAGCT TCCCGGCGCT CCGCCGGCCC AGCGCGCGGG GTCCCCACCC
GATGCTCCGG GCTCCGAGAG CCCCGCGCTT GCCTGCAGCA CTCCGGCCAC GCCCAGCGGC
GAGGACCCGC CAGCCCGAGC AGCACCCATC GCCCCGCGGC CCCCCGCCAG GCCTCGACTG
GAGCGAGCCC TGTCCCTGGA CGACAAGGGC TGGAGGAGGA GGCGTTTTCG AGGCAGCCAG
GAGGACCTGG AAGCCCGGAA TGGGACCAGT CCCTCCAGGG GCTCAGTGCA GAGCGAGGGC
CCCGGGGCCC CCGCCCACAG CTGCTCCCCG CCCTGCCTGA GCACCTCCTT GCAGGAAATC
CCCAAGTCCC GCGGGGTCCT GAGCAGTGAG AGAGGGAGCC CGTCCTCGGG GGGTAACCCT
CTCTCTGGGG TGGCCAGCAG CTCCCCGAAC CTCCCGCACA GAGACGCCGC CGTGGCGGGG
AGCTCGCCCA GGCTGCCCAG CCTGCTGCCC CCGCGCCCAC CGCCTGCCCT GAGCCTGGAC
ATCGCCTCCG ACTCCCTGAG GACAGCAAAC AAGGTCGACT CGGATCTTGC AGACTACAAG
CTCCGCGCGC AGCCGCTCCT GGTGCGGGCC CACAGCAGCC TGGGCCCCGG CCGGCCGCGG
AGCCCCCTGG CCTGCGACGA CTGTTCCCTT CGCTCGGCCA AATCCTCCTT CAGCCTCCTG
GCGCCCATCC GCAGCAAGGA CGTCCGCAGC AGGAGTTACC TGGAGGGCAG CCTCCTGGCC
AGCGGGGCCC TGTTGGGGGC GGATGAGCTG GCCCGCTACT TCCCAGACCG GAACGTGGCA
CTCTTCGTGG CCACCTGGAA CATGCAGGGC CAGAAGGAGC TCCCGCCCAG CCTGGACGAG
TTCCTGCTCC CAGCCGAGGC CGACTATGCC CAGGACCTGT ATGTCATCGG GGTCCAGGAG
GGCTGTTCTG ACAGGCGGGA GTGGGAGACT CGTCTGCAGG AGACGCTGGG CCCGCACTAT
GTGCTGCTGT CCTCGGCGGC CCACGGCGTG CTCTACATGT CGCTCTTCAT CCGCAGGGAC
CTCATCTGGT TCTGCTCAGA GGTGGAGTGC TCCACGGTGA CCACACGCAT CGTGTCTCAG
ATCAAGACCA AGGGGGCCTT GGGCATCAGC TTCACCTTTT TTGGCACTTC CTTCCTCTTC
ATCACGTCCC ACTTCACCTC AGGTGACGGG AAGGTGGCGG AGCGGCTGCT GGACTACACC
AGGACTGTAC AAGCCCTGGT CCTGCCCAGA AATGTGCCCG ACACCAACCC CTATCGCTCC
AGCGCAGCGG ACGTCACCAC CCGCTTCGAT GAGGTGTTCT GGTTTGGAGA CTTCAACTTC
CGCCTGAGTG GCGGGCGCAC AGTCGTGGAC GCCCTCCTGT GCCAGGGCCT GGTGGTGGAC
GTGCCGGCGC TGCTGCAGCA CGACCAGCTC ATCCGGGAGA TGCGGAAAGG GTCCATCTTC
AAGGGCTTCC AGGAGCCGGA CATCCACTTC CTCCCATCAT ACAAGTTTGA CATCGGGAAG
GACACGTACG ACAGCACCTC CAAGCAGAGG ACGCCCTCAT ACACGGACCG CGTCTTGTAC
AGAAGCCGCC ACAAGGGTGA CATCTGTCCT GTGAGCTACT CTTCCTGCCC CGGGATCAAG
ACGTCCGACC ACCGCCCTGT GTATGGCCTC TTCCGGGTGA AAGTGAGGCC GGGGCGAGAC
AACATTCCGT TGGCAGCTGG CAAATTTGAT AGAGAACTGT ACTTACTAGG AATTAAAAGA
CGGATTTCGA AGGAGATTCA GAGGCAGCAA GCACTACAGA GTCAGAACTC CAGCACCATC
TGCTCCGTTT CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_063945.2
CDS404..2338
Misc Feature(1)347..349
Misc Feature(2)431..1129
Misc Feature(3)698..700
Misc Feature(4)698..700
Misc Feature(5)1124..1126
Misc Feature(6)1169..1171
Misc Feature(7)1286..2182
Misc Feature(8)order(1322..1324,1328..1330,1421..1423,1610..1615,1673..1675,1682..1687,1832..1834,1838..1840,1844..1846,2003..2008,2051..2053,2069..2071,2150..2155)
Misc Feature(9)order(1322..1324,1421..1423,1673..1675,1832..1834,1838..1840,2069..2071,2150..2155)
Misc Feature(10)order(1421..1423,1610..1615,1673..1675,1682..1687,1844..1846,2003..2008,2051..2053,2150..2152)
Misc Feature(11)order(1421..1423,2150..2152)
Misc Feature(12)order(1673..1675,1838..1840,2153..2155)
Misc Feature(13)2324..2326
Exon (1)1..1215
Exon (2)1216..1339
Exon (3)1340..1437
Exon (4)1438..1562
Exon (5)1563..1682
Exon (6)1683..1790
Exon (7)1791..1952
Exon (8)1953..2068
Exon (9)2069..2205
Exon (10)2206..3398
Translation

Target ORF information:

RefSeq Version NM_019892.5
Organism Homo sapiens(Human)
Definition Homo sapiens inositol polyphosphate-5-phosphatase E (INPP5E), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_019892.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCCGTCCA AGGCGGAGAA TCTGCGGCCC TCCGAGCCGG CCCCGCAGCC GCCGGAAGGG 
AGGACGCTCC AAGGACAGCT TCCCGGCGCT CCGCCGGCCC AGCGCGCGGG GTCCCCACCC
GATGCTCCGG GCTCCGAGAG CCCCGCGCTT GCCTGCAGCA CTCCGGCCAC GCCCAGCGGC
GAGGACCCGC CAGCCCGAGC AGCACCCATC GCCCCGCGGC CCCCCGCCAG GCCTCGACTG
GAGCGAGCCC TGTCCCTGGA CGACAAGGGC TGGAGGAGGA GGCGTTTTCG AGGCAGCCAG
GAGGACCTGG AAGCCCGGAA TGGGACCAGT CCCTCCAGGG GCTCAGTGCA GAGCGAGGGC
CCCGGGGCCC CCGCCCACAG CTGCTCCCCG CCCTGCCTGA GCACCTCCTT GCAGGAAATC
CCCAAGTCCC GCGGGGTCCT GAGCAGTGAG AGAGGGAGCC CGTCCTCGGG GGGTAACCCT
CTCTCTGGGG TGGCCAGCAG CTCCCCGAAC CTCCCGCACA GAGACGCCGC CGTGGCGGGG
AGCTCGCCCA GGCTGCCCAG CCTGCTGCCC CCGCGCCCAC CGCCTGCCCT GAGCCTGGAC
ATCGCCTCCG ACTCCCTGAG GACAGCAAAC AAGGTCGACT CGGATCTTGC AGACTACAAG
CTCCGCGCGC AGCCGCTCCT GGTGCGGGCC CACAGCAGCC TGGGCCCCGG CCGGCCGCGG
AGCCCCCTGG CCTGCGACGA CTGTTCCCTT CGCTCGGCCA AATCCTCCTT CAGCCTCCTG
GCGCCCATCC GCAGCAAGGA CGTCCGCAGC AGGAGTTACC TGGAGGGCAG CCTCCTGGCC
AGCGGGGCCC TGTTGGGGGC GGATGAGCTG GCCCGCTACT TCCCAGACCG GAACGTGGCA
CTCTTCGTGG CCACCTGGAA CATGCAGGGC CAGAAGGAGC TCCCGCCCAG CCTGGACGAG
TTCCTGCTCC CAGCCGAGGC CGACTATGCC CAGGACCTGT ATGTCATCGG GGTCCAGGAG
GGCTGTTCTG ACAGGCGGGA GTGGGAGACT CGTCTGCAGG AGACGCTGGG CCCGCACTAT
GTGCTGCTGT CCTCGGCGGC CCACGGCGTG CTCTACATGT CGCTCTTCAT CCGCAGGGAC
CTCATCTGGT TCTGCTCAGA GGTGGAGTGC TCCACGGTGA CCACACGCAT CGTGTCTCAG
ATCAAGACCA AGGGGGCCTT GGGCATCAGC TTCACCTTTT TTGGCACTTC CTTCCTCTTC
ATCACGTCCC ACTTCACCTC AGGTGACGGG AAGGTGGCGG AGCGGCTGCT GGACTACACC
AGGACTGTAC AAGCCCTGGT CCTGCCCAGA AATGTGCCCG ACACCAACCC CTATCGCTCC
AGCGCAGCGG ACGTCACCAC CCGCTTCGAT GAGGTGTTCT GGTTTGGAGA CTTCAACTTC
CGCCTGAGTG GCGGGCGCAC AGTCGTGGAC GCCCTCCTGT GCCAGGGCCT GGTGGTGGAC
GTGCCGGCGC TGCTGCAGCA CGACCAGCTC ATCCGGGAGA TGCGGAAAGG GTCCATCTTC
AAGGGCTTCC AGGAGCCGGA CATCCACTTC CTCCCATCAT ACAAGTTTGA CATCGGGAAG
GACACGTACG ACAGCACCTC CAAGCAGAGG ACGCCCTCAT ACACGGACCG CGTCTTGTAC
AGAAGCCGCC ACAAGGGTGA CATCTGTCCT GTGAGCTACT CTTCCTGCCC CGGGATCAAG
ACGTCCGACC ACCGCCCTGT GTATGGCCTC TTCCGGGTGA AAGTGAGGCC GGGGCGAGAC
AACATTCCGT TGGCAGCTGG CAAATTTGAT AGAGAACTGT ACTTACTAGG AATTAAAAGA
CGGATTTCGA AGGAGATTCA GAGGCAGCAA GCACTACAGA GTCAGAACTC CAGCACCATC
TGCTCCGTTT CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43395
Clone ID Related Accession (Same CDS sequence) NM_001318502.1
Accession Version NM_001318502.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-13
Organism Homo sapiens(Human)
Product 72 kDa inositol polyphosphate 5-phosphatase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832846.1, BC028032.1, AL592301.14 and CA436481.1. On Jan 8, 2016 this sequence version replaced XM_005266094.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in an internal exon, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832846.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCCGTCCA AGGCGGAGAA TCTGCGGCCC TCCGAGCCGG CCCCGCAGCC GCCGGAAGGG 
AGGACGCTCC AAGGACAGCT TCCCGGCGCT CCGCCGGCCC AGCGCGCGGG GTCCCCACCC
GATGCTCCGG GCTCCGAGAG CCCCGCGCTT GCCTGCAGCA CTCCGGCCAC GCCCAGCGGC
GAGGACCCGC CAGCCCGAGC AGCACCCATC GCCCCGCGGC CCCCCGCCAG GCCTCGACTG
GAGCGAGCCC TGTCCCTGGA CGACAAGGGC TGGAGGAGGA GGCGTTTTCG AGGCAGCCAG
GAGGACCTGG AAGCCCGGAA TGGGACCAGT CCCTCCAGGG GCTCAGTGCA GAGCGAGGGC
CCCGGGGCCC CCGCCCACAG CTGCTCCCCG CCCTGCCTGA GCACCTCCTT GCAGGAAATC
CCCAAGTCCC GCGGGGTCCT GAGCAGTGAG AGAGGGAGCC CGTCCTCGGG GGGTAACCCT
CTCTCTGGGG TGGCCAGCAG CTCCCCGAAC CTCCCGCACA GAGACGCCGC CGTGGCGGGG
AGCTCGCCCA GGCTGCCCAG CCTGCTGCCC CCGCGCCCAC CGCCTGCCCT GAGCCTGGAC
ATCGCCTCCG ACTCCCTGAG GACAGCAAAC AAGGTCGACT CGGATCTTGC AGACTACAAG
CTCCGCGCGC AGCCGCTCCT GGTGCGGGCC CACAGCAGCC TGGGCCCCGG CCGGCCGCGG
AGCCCCCTGG CCTGCGACGA CTGTTCCCTT CGCTCGGCCA AATCCTCCTT CAGCCTCCTG
GCGCCCATCC GCAGCAAGGA CGTCCGCAGC AGGAGTTACC TGGAGGGCAG CCTCCTGGCC
AGCGGGGCCC TGTTGGGGGC GGATGAGCTG GCCCGCTACT TCCCAGACCG GAACGTGGCA
CTCTTCGTGG CCACCTGGAA CATGCAGGGC CAGAAGGAGC TCCCGCCCAG CCTGGACGAG
TTCCTGCTCC CAGCCGAGGC CGACTATGCC CAGGACCTGT ATGTCATCGG GGTCCAGGAG
GGCTGTTCTG ACAGGCGGGA GTGGGAGACT CGTCTGCAGG AGACGCTGGG CCCGCACTAT
GTGCTGCTGT CCTCGGCGGC CCACGGCGTG CTCTACATGT CGCTCTTCAT CCGCAGGGAC
CTCATCTGGT TCTGCTCAGA GGTGGAGTGC TCCACGGTGA CCACACGCAT CGTGTCTCAG
ATCAAGACCA AGGGGGCCTT GGGCATCAGC TTCACCTTTT TTGGCACTTC CTTCCTCTTC
ATCACGTCCC ACTTCACCTG TGACGGGAAG GTGGCGGAGC GGCTGCTGGA CTACACCAGG
ACTGTACAAG CCCTGGTCCT GCCCAGAAAT GTGCCCGACA CCAACCCCTA TCGCTCCAGC
GCAGCGGACG TCACCACCCG CTTCGATGAG GTGTTCTGGT TTGGAGACTT CAACTTCCGC
CTGAGTGGCG GGCGCACAGT CGTGGACGCC CTCCTGTGCC AGGGCCTGGT GGTGGACGTG
CCGGCGCTGC TGCAGCACGA CCAGCTCATC CGGGAGATGC GGAAAGGGTC CATCTTCAAG
GGCTTCCAGG AGCCGGACAT CCACTTCCTC CCATCATACA AGTTTGACAT CGGGAAGGAC
ACGTACGACA GCACCTCCAA GCAGAGGACG CCCTCATACA CGGACCGCGT CTTGTACAGA
AGCCGCCACA AGGGTGACAT CTGTCCTGTG AGCTACTCTT CCTGCCCCGG GATCAAGACG
TCCGACCACC GCCCTGTGTA TGGCCTCTTC CGGGTGAAAG TGAGGCCGGG GCGAGACAAC
ATTCCGTTGG CAGCTGGCAA ATTTGATAGA GAACTGTACT TACTAGGAAT TAAAAGACGG
ATTTCGAAGG AGATTCAGAG GCAGCAAGCA CTACAGAGTC AGAACTCCAG CACCATCTGC
TCCGTTTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001305431.1
CDS404..2335
Misc Feature(1)347..349
Misc Feature(2)431..1129
Misc Feature(3)698..700
Misc Feature(4)1124..1126
Misc Feature(5)1169..1171
Misc Feature(6)1286..2179
Misc Feature(7)order(1322..1324,1328..1330,1421..1423,1610..1615,1673..1675,1682..1684,1829..1831,1835..1837,1841..1843,2000..2005,2048..2050,2066..2068,2147..2152)
Misc Feature(8)order(1322..1324,1421..1423,1673..1675,1829..1831,1835..1837,2066..2068,2147..2152)
Misc Feature(9)order(1421..1423,1610..1615,1673..1675,1682..1684,1841..1843,2000..2005,2048..2050,2147..2149)
Misc Feature(10)order(1421..1423,2147..2149)
Misc Feature(11)order(1673..1675,1835..1837,2150..2152)
Misc Feature(12)2321..2323
Exon (1)1..1215
Exon (2)1216..1339
Exon (3)1340..1437
Exon (4)1438..1562
Exon (5)1563..1682
Exon (6)1683..1787
Exon (7)1788..1949
Exon (8)1950..2065
Exon (9)2066..2202
Exon (10)2203..3395
Translation

Target ORF information:

RefSeq Version NM_001318502.1
Organism Homo sapiens(Human)
Definition Homo sapiens inositol polyphosphate-5-phosphatase E (INPP5E), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001318502.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCCGTCCA AGGCGGAGAA TCTGCGGCCC TCCGAGCCGG CCCCGCAGCC GCCGGAAGGG 
AGGACGCTCC AAGGACAGCT TCCCGGCGCT CCGCCGGCCC AGCGCGCGGG GTCCCCACCC
GATGCTCCGG GCTCCGAGAG CCCCGCGCTT GCCTGCAGCA CTCCGGCCAC GCCCAGCGGC
GAGGACCCGC CAGCCCGAGC AGCACCCATC GCCCCGCGGC CCCCCGCCAG GCCTCGACTG
GAGCGAGCCC TGTCCCTGGA CGACAAGGGC TGGAGGAGGA GGCGTTTTCG AGGCAGCCAG
GAGGACCTGG AAGCCCGGAA TGGGACCAGT CCCTCCAGGG GCTCAGTGCA GAGCGAGGGC
CCCGGGGCCC CCGCCCACAG CTGCTCCCCG CCCTGCCTGA GCACCTCCTT GCAGGAAATC
CCCAAGTCCC GCGGGGTCCT GAGCAGTGAG AGAGGGAGCC CGTCCTCGGG GGGTAACCCT
CTCTCTGGGG TGGCCAGCAG CTCCCCGAAC CTCCCGCACA GAGACGCCGC CGTGGCGGGG
AGCTCGCCCA GGCTGCCCAG CCTGCTGCCC CCGCGCCCAC CGCCTGCCCT GAGCCTGGAC
ATCGCCTCCG ACTCCCTGAG GACAGCAAAC AAGGTCGACT CGGATCTTGC AGACTACAAG
CTCCGCGCGC AGCCGCTCCT GGTGCGGGCC CACAGCAGCC TGGGCCCCGG CCGGCCGCGG
AGCCCCCTGG CCTGCGACGA CTGTTCCCTT CGCTCGGCCA AATCCTCCTT CAGCCTCCTG
GCGCCCATCC GCAGCAAGGA CGTCCGCAGC AGGAGTTACC TGGAGGGCAG CCTCCTGGCC
AGCGGGGCCC TGTTGGGGGC GGATGAGCTG GCCCGCTACT TCCCAGACCG GAACGTGGCA
CTCTTCGTGG CCACCTGGAA CATGCAGGGC CAGAAGGAGC TCCCGCCCAG CCTGGACGAG
TTCCTGCTCC CAGCCGAGGC CGACTATGCC CAGGACCTGT ATGTCATCGG GGTCCAGGAG
GGCTGTTCTG ACAGGCGGGA GTGGGAGACT CGTCTGCAGG AGACGCTGGG CCCGCACTAT
GTGCTGCTGT CCTCGGCGGC CCACGGCGTG CTCTACATGT CGCTCTTCAT CCGCAGGGAC
CTCATCTGGT TCTGCTCAGA GGTGGAGTGC TCCACGGTGA CCACACGCAT CGTGTCTCAG
ATCAAGACCA AGGGGGCCTT GGGCATCAGC TTCACCTTTT TTGGCACTTC CTTCCTCTTC
ATCACGTCCC ACTTCACCTG TGACGGGAAG GTGGCGGAGC GGCTGCTGGA CTACACCAGG
ACTGTACAAG CCCTGGTCCT GCCCAGAAAT GTGCCCGACA CCAACCCCTA TCGCTCCAGC
GCAGCGGACG TCACCACCCG CTTCGATGAG GTGTTCTGGT TTGGAGACTT CAACTTCCGC
CTGAGTGGCG GGCGCACAGT CGTGGACGCC CTCCTGTGCC AGGGCCTGGT GGTGGACGTG
CCGGCGCTGC TGCAGCACGA CCAGCTCATC CGGGAGATGC GGAAAGGGTC CATCTTCAAG
GGCTTCCAGG AGCCGGACAT CCACTTCCTC CCATCATACA AGTTTGACAT CGGGAAGGAC
ACGTACGACA GCACCTCCAA GCAGAGGACG CCCTCATACA CGGACCGCGT CTTGTACAGA
AGCCGCCACA AGGGTGACAT CTGTCCTGTG AGCTACTCTT CCTGCCCCGG GATCAAGACG
TCCGACCACC GCCCTGTGTA TGGCCTCTTC CGGGTGAAAG TGAGGCCGGG GCGAGACAAC
ATTCCGTTGG CAGCTGGCAA ATTTGATAGA GAACTGTACT TACTAGGAAT TAAAAGACGG
ATTTCGAAGG AGATTCAGAG GCAGCAAGCA CTACAGAGTC AGAACTCCAG CACCATCTGC
TCCGTTTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.
Nature communications710777(2016 Feb)
Xu Q,Zhang Y,Wei Q,Huang Y,Hu J,Ling K


book

INPP5E interacts with AURKA, linking phosphoinositide signaling to primary cilium stability.
Journal of cell science128(2)364-72(2015 Jan)
Plotnikova OV,Seo S,Cottle DL,Conduit S,Hakim S,Dyson JM,Mitchell CA,Smyth IM


book

A homozygous PDE6D mutation in Joubert syndrome impairs targeting of farnesylated INPP5E protein to the primary cilium.
Human mutation35(1)137-46(2014 Jan)
Thomas S,Wright KJ,Le Corre S,Micalizzi A,Romani M,Abhyankar A,Saada J,Perrault I,Amiel J,Litzler J,Filhol E,Elkhartoufi N,Kwong M,Casanova JL,Boddaert N,Baehr W,Lyonnet S,Munnich A,Burglen L,Chassaing N,Encha-Ravazi F,Vekemans M,Gleeson JG,Valente EM,Jackson PK,Drummond IA,Saunier S,Attié-Bitach T


book

Phenotypic spectrum and prevalence of INPP5E mutations in Joubert syndrome and related disorders.
European journal of human genetics : EJHG21(10)1074-8(2013 Oct)
Travaglini L,Brancati F,Silhavy J,Iannicelli M,Nickerson E,Elkhartoufi N,Scott E,Spencer E,Gabriel S,Thomas S,Ben-Zeev B,Bertini E,Boltshauser E,Chaouch M,Cilio MR,de Jong MM,Kayserili H,Ogur G,Poretti A,Signorini S,Uziel G,Zaki MS,,Johnson C,Attié-Bitach T,Gleeson JG,Valente EM


 
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